CN119015182B - A moisturizing composition and its preparation method and application - Google Patents
A moisturizing composition and its preparation method and application Download PDFInfo
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- CN119015182B CN119015182B CN202411513975.4A CN202411513975A CN119015182B CN 119015182 B CN119015182 B CN 119015182B CN 202411513975 A CN202411513975 A CN 202411513975A CN 119015182 B CN119015182 B CN 119015182B
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- extract
- inonotus obliquus
- moisturizing composition
- moisturizing
- inonotus
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- 239000000203 mixture Substances 0.000 title claims abstract description 54
- 230000003020 moisturizing effect Effects 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 239000000284 extract Substances 0.000 claims abstract description 69
- YRCWQPVGYLYSOX-UHFFFAOYSA-N synephrine Chemical compound CNCC(O)C1=CC=C(O)C=C1 YRCWQPVGYLYSOX-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000001963 growth medium Substances 0.000 claims abstract description 27
- 238000000855 fermentation Methods 0.000 claims abstract description 25
- 230000004151 fermentation Effects 0.000 claims abstract description 25
- 239000002609 medium Substances 0.000 claims abstract description 24
- 229960003684 oxedrine Drugs 0.000 claims abstract description 24
- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 claims abstract description 24
- 238000011218 seed culture Methods 0.000 claims abstract description 22
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 18
- 102000011632 Caseins Human genes 0.000 claims abstract description 16
- 108010076119 Caseins Proteins 0.000 claims abstract description 16
- 229940080237 sodium caseinate Drugs 0.000 claims abstract description 16
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000014104 aloe vera supplement Nutrition 0.000 claims abstract description 10
- 229940119217 chamomile extract Drugs 0.000 claims abstract description 9
- 235000020221 chamomile extract Nutrition 0.000 claims abstract description 9
- 229940064064 purslane extract Drugs 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 229940015975 1,2-hexanediol Drugs 0.000 claims abstract description 6
- 240000008632 Cota tinctoria Species 0.000 claims abstract description 6
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims abstract description 6
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 claims abstract description 6
- 240000007707 Mentha arvensis Species 0.000 claims abstract description 3
- 235000018978 Mentha arvensis Nutrition 0.000 claims abstract description 3
- 241000414067 Inonotus obliquus Species 0.000 claims description 71
- 239000000047 product Substances 0.000 claims description 31
- HWDGVJUIHRPKFR-UHFFFAOYSA-I copper;trisodium;18-(2-carboxylatoethyl)-20-(carboxylatomethyl)-12-ethenyl-7-ethyl-3,8,13,17-tetramethyl-17,18-dihydroporphyrin-21,23-diide-2-carboxylate Chemical compound [Na+].[Na+].[Na+].[Cu+2].N1=C(C(CC([O-])=O)=C2C(C(C)C(C=C3C(=C(C=C)C(=C4)[N-]3)C)=N2)CCC([O-])=O)C(=C([O-])[O-])C(C)=C1C=C1C(CC)=C(C)C4=N1 HWDGVJUIHRPKFR-UHFFFAOYSA-I 0.000 claims description 15
- 229940079841 sodium copper chlorophyllin Drugs 0.000 claims description 15
- 235000013758 sodium copper chlorophyllin Nutrition 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 235000016709 nutrition Nutrition 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 9
- 230000035764 nutrition Effects 0.000 claims description 9
- 238000010992 reflux Methods 0.000 claims description 9
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- 238000000605 extraction Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
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- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 7
- NOPFSRXAKWQILS-UHFFFAOYSA-N docosan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCCCCCO NOPFSRXAKWQILS-UHFFFAOYSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 235000015278 beef Nutrition 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 229920002125 Sokalan® Polymers 0.000 claims description 3
- 229960001631 carbomer Drugs 0.000 claims description 3
- 239000004359 castor oil Substances 0.000 claims description 3
- 235000019438 castor oil Nutrition 0.000 claims description 3
- 229960000735 docosanol Drugs 0.000 claims description 3
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 abstract description 15
- 229920001282 polysaccharide Polymers 0.000 abstract description 15
- 239000005017 polysaccharide Substances 0.000 abstract description 15
- 238000012360 testing method Methods 0.000 abstract description 13
- 235000018185 Betula X alpestris Nutrition 0.000 abstract description 4
- 235000018212 Betula X uliginosa Nutrition 0.000 abstract description 4
- 241000123247 Inonotus Species 0.000 abstract 3
- ZIALXKMBHWELGF-UHFFFAOYSA-N [Na].[Cu] Chemical compound [Na].[Cu] ZIALXKMBHWELGF-UHFFFAOYSA-N 0.000 abstract 1
- 229930002875 chlorophyll Natural products 0.000 abstract 1
- 235000019804 chlorophyll Nutrition 0.000 abstract 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 abstract 1
- 229940084750 sophora flavescens root extract Drugs 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 31
- 230000000052 comparative effect Effects 0.000 description 20
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- 238000011081 inoculation Methods 0.000 description 7
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- 239000004744 fabric Substances 0.000 description 5
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- 239000004480 active ingredient Substances 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 239000001771 mentha piperita Substances 0.000 description 4
- 239000002390 adhesive tape Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 3
- 235000019797 dipotassium phosphate Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
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- 206010040880 Skin irritation Diseases 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 2
- 210000000245 forearm Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000036556 skin irritation Effects 0.000 description 2
- 231100000475 skin irritation Toxicity 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 241000222382 Agaricomycotina Species 0.000 description 1
- 240000000643 Alnus japonica Species 0.000 description 1
- 244000144927 Aloe barbadensis Species 0.000 description 1
- 235000002961 Aloe barbadensis Nutrition 0.000 description 1
- 235000009109 Betula pendula Nutrition 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000222383 Polyporales Species 0.000 description 1
- 206010040914 Skin reaction Diseases 0.000 description 1
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- 238000002835 absorbance Methods 0.000 description 1
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- 235000011399 aloe vera Nutrition 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000001153 anti-wrinkle effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
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- 238000006703 hydration reaction Methods 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
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- 231100000344 non-irritating Toxicity 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
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- 150000003431 steroids Chemical class 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 240000004494 yellow birch Species 0.000 description 1
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- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K8/35—Ketones, e.g. benzophenone
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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Abstract
本发明属于护肤品技术领域，具体涉及一种保湿组合物及其制备方法和应用。该组合物由以下质量百分含量的原料组成：桦褐孔菌提取物5‑10%、马齿苋提取物6‑15%、库拉索芦荟提取物3‑5%、苦参根提取物1‑3%、田野薄荷叶提取物7‑9%、金黄洋甘菊提取物10‑12%、丁二醇15‑20%、甘油8‑12%、1,2‑己二醇0.5‑1%、对羟基苯乙酮0.3‑0.5%、余量为水。本发明在桦褐孔菌提取物的制备过程中，创新性地在基础种子培养基中添加了辛弗林、酪朊酸钠，在发酵培养基中添加了莽草酸、叶绿素铜钠盐。试验结果表明，辛弗林、莽草酸的加入有效提升了桦褐孔菌提取物中多糖含量，进而提高了护肤品的保湿效果。The present invention belongs to the technical field of skin care products, and specifically relates to a moisturizing composition and its preparation method and application. The composition is composed of the following raw materials in percentage by mass: 5-10% of birch inonotus extract, 6-15% of purslane extract, 3-5% of aloe vera extract, 1-3% of sophora flavescens root extract, 7-9% of field mint leaf extract, 10-12% of golden chamomile extract, 15-20% of butanediol, 8-12% of glycerol, 0.5-1% of 1,2-hexanediol, 0.3-0.5% of p-hydroxyacetophenone, and the balance is water. In the preparation process of birch inonotus extract, the present invention innovatively adds synephrine and sodium caseinate to the basic seed culture medium, and adds shikimic acid and sodium copper chlorophyll to the fermentation medium. The test results show that the addition of synephrine and shikimic acid effectively increases the polysaccharide content in birch inonotus extract, thereby improving the moisturizing effect of skin care products.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to a moisturizing composition, a preparation method and application thereof.
Background
The whole body moisture of a person is mainly stored in skin, and various skin problems such as dryness, dullness, roughness and the like can be caused when the skin is in a water shortage condition. And with the improvement of living conditions and working demands, people are in a closed air-conditioning room for a long time, which can lead to the acceleration of the evaporation of skin moisture and the physiological decrease of skin moisture with the increase of age. In addition, external environmental factors such as season exchanges have a great influence on skin moisture. Based on this, there is a great demand for skin moisturizing products.
Inonotus obliquus (Inonotus obliquus) belongs to Basidiomycotina, hymenomycetes, aphyllophorales, phanerochaeteceae, and is mainly parasitic under living standing tree bark of birch, silver birch, elm, alnus japonica, etc. or on stem of cut tree. It is mainly distributed in the northern hemisphere in the region of 45-50 degrees. Inonotus obliquus is mainly distributed in Changbai mountain area of Heilongjiang province and Jilin province in China. The research shows that the inonotus obliquus extract contains a plurality of active ingredients such as polysaccharide, triterpene, steroid, polyphenol and the like. In addition, the inonotus obliquus extract has remarkable antioxidant and anti-inflammatory effects, can improve the skin texture and reduce fine wrinkles, and has certain anti-aging and anti-wrinkle effects. Chinese patent CN117815096A discloses a moisturizing and repairing composition and application thereof, and discloses an inonotus obliquus extract with moisturizing effect. Chinese patent CN117398326A discloses a skin moisturizing and repairing composition and application thereof, and discloses that inonotus obliquus extract can effectively strengthen the cohesive force of keratinocytes and strengthen the skin barrier function.
Although various preparation methods of inonotus obliquus extracts have been disclosed in the prior art, there is still room for improvement in the content of active ingredients and skin care effect. Chinese patent CN117398326A discloses a skin moisturizing and repairing composition and application thereof, and also discloses an inonotus obliquus extract obtained by adopting a subcritical water extraction process, wherein the method has better moisturizing and repairing effects compared with an organic supercritical extraction process and a traditional water-alcohol extraction process. The extraction process can extract the active ingredients with moisturizing effect, but has the problems of low extraction efficiency, insufficient content of the active ingredients and the like, and particularly, the polysaccharide content is difficult to meet the skin care requirement.
Based on the above, improvement is needed for the culture process and extraction process of inonotus obliquus, and the content of polysaccharide in inonotus obliquus is remarkably enhanced, so that the application of inonotus obliquus in the field of skin care products is accelerated.
Disclosure of Invention
A first object of the present invention is to provide a moisturizing composition having excellent moisturizing effect.
A second object of the present invention is to provide a method for preparing a moisturizing composition which is simple and easy to handle.
The third object of the invention is to provide an application of the moisturizing composition, which has wide application prospect.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
A moisturizing composition comprises, by mass, 5-10% of Inonotus obliquus extract, 6-15% of purslane extract, 3-5% of aloe vera extract, 1-3% of radix sophorae flavescentis extract, 7-9% of herba menthae in field, 10-12% of chamomile extract, 15-20% of butanediol, 8-12% of glycerol, 0.5-1% of 1, 2-hexanediol, 0.3-0.5% of p-hydroxyacetophenone, and the balance of water;
the preparation process of the inonotus obliquus extract comprises the following steps:
(1) Inoculating the inonotus obliquus strain into a basic seed culture medium added with sodium caseinate and synephrine for culturing to obtain inonotus obliquus mycelium;
(2) Inoculating the inonotus obliquus mycelium obtained in the step (1) to a fermentation medium added with shikimic acid and sodium copper chlorophyllin for continuous culture to obtain a product;
(3) Crushing the product obtained in the step (2), adding water for reflux extraction to obtain an extracting solution, filtering and concentrating the extracting solution, adding ethanol for precipitation, collecting the precipitate, and drying the precipitate to obtain the inonotus obliquus extract.
Further, the concentration of the sodium caseinate and the synephrine in the basic seed culture medium in the step (1) is 0.08-0.15g/L of sodium caseinate and 1-5mg/L of synephrine, and the nutrition composition of the basic seed culture medium is 15-20g/L of glucose, 1-5g/L of peptone, 0.5.5-1.5 g/L of potassium dihydrogen phosphate and 0.5-1.5g/L of anhydrous magnesium sulfate.
Further, the inoculum size of the inonotus obliquus strain in the step (1) is 8-10% of the total mass of the basic seed culture medium, the culture temperature is 20-30 ℃, and the culture time is 7-15d.
Further, the concentration of shikimic acid and sodium copper chlorophyllin in the step (2) in the fermentation culture medium is 9-15mg/L of shikimic acid and 0.4-1mg/L of sodium copper chlorophyllin, and the nutrition composition of the fermentation culture medium is 15-20g/L of glucose, 2-6g/L of beef extract, -1.5g/L of dipotassium hydrogen phosphate and 0.5-1.5g/L of anhydrous magnesium sulfate and 0.05-0.15g/L of L, mnSO 4·H2 O.
Further, the inoculation amount of the inonotus obliquus mycelium in the step (2) is 8-12% of the total mass of the fermentation medium, the temperature of continuous culture is 20-28 ℃, and the time is 10-14d.
Further, the reflux extraction time in the step (3) is 2-3h.
The preparation method of the moisturizing composition comprises the following steps:
and uniformly mixing the raw materials according to the mass percentage.
The application of the moisturizing composition in preparing skin care products.
Further, the moisture-keeping composition is 1-5% by mass based on the total mass of the skin care product.
Further, the skin care product comprises the following raw materials, by mass, 0.1-0.3% of carbomer, 4-6% of PEG-40 hydrogenated castor oil, 0.5-1% of behenyl alcohol and the balance of water.
Compared with the prior art, the invention has the following main beneficial effects:
1. In the preparation process of the inonotus obliquus extract, synephrine and sodium caseinate are innovatively added into a basic seed culture medium, and shikimic acid and sodium copper chlorophyllin are added into a fermentation culture medium. The polysaccharide content test result shows that the addition of synephrine and shikimic acid can effectively improve the polysaccharide content in the inonotus obliquus extract, and the polysaccharide content in the inonotus obliquus extract reaches more than 35.56 weight percent.
2. The moisturizing composition provided by the invention is mild and has no stimulation to skin, is safe to use, and has an excellent moisturizing effect. The skin moisture test results show that the skin hydration content change rate reaches more than 68.47% after the skin care product 28d added with the moisturizing composition of the present invention is used.
Detailed Description
The technical scheme of the invention is further described below with reference to the specific embodiments. It will be understood by those skilled in the art that the following examples are illustrative of the present invention and are not to be construed as limiting the invention. The specific conditions not specified in the examples were carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used, unless otherwise specified, are all conventional products obtained from commercial sources.
Examples
Example 1
A moisturizing composition comprises, by mass, 8% of inonotus obliquus extract, 12% of purslane extract, 4% of aloe vera extract, 2% of radix sophorae flavescentis extract, 8% of peppermint leaf extract, 11% of chamomile extract, 16% of butanediol, 9% of glycerol, 0.8% of 1, 2-hexanediol, 0.4% of p-hydroxyacetophenone and the balance of water.
The preparation method of the inonotus obliquus extract comprises the following steps:
(1) According to the inoculation amount of the inonotus obliquus strain as 9% of the total mass of a basic seed culture medium, inoculating the inonotus obliquus strain into the basic seed culture medium added with sodium caseinate and synephrine, wherein the concentration of the sodium caseinate and the synephrine in the basic seed culture medium is 0.1g/L of sodium caseinate and 4mg/L of synephrine, and culturing for 12d at 28 ℃ to obtain inonotus obliquus mycelium, and the nutrition of the basic seed culture medium comprises 18g/L of glucose, 4g/L of peptone, g/L of monopotassium phosphate and 1g/L of anhydrous magnesium sulfate.
(2) Inoculating Inonotus obliquus mycelium obtained in the step (1) into a fermentation medium added with shikimic acid and sodium copper chlorophyllin according to the inoculation amount of the Inonotus obliquus mycelium accounting for 10% of the total mass of the fermentation medium, wherein the concentration of the shikimic acid and the sodium copper chlorophyllin in the fermentation medium is 12mg/L shikimic acid and 0.5mg/L sodium copper chlorophyllin, and continuously culturing for 12d at 25 ℃ to obtain a product, and the nutrition composition of the fermentation medium is 18g/L glucose, 5g/L beef extract, g/L dipotassium phosphate and 1g/L, mnSO 4·H2 O0.1 g/L anhydrous magnesium sulfate.
(3) Pulverizing the product obtained in the step (2) to a granularity of 5mm, adding water, extracting under reflux for 2.5h to obtain an extracting solution, filtering and concentrating the extracting solution, adding ethanol to precipitate, collecting the precipitate, and drying the precipitate to obtain the inonotus obliquus extract.
The preparation method of the purslane extract comprises the following steps:
Squeezing fresh herba Portulacae at 45deg.C for 10min to obtain juice and herba Portulacae residue, adding 60% (v/v) ethanol water solution 8 times the weight of herba Portulacae residue to the residue, performing solid-liquid separation to obtain extractive solution A and residue, adding 8 times the weight of water to residue, performing solid-liquid separation to obtain extractive solution B, mixing the juice, extractive solution A and extractive solution B, filtering with 0.45 μm filter membrane to obtain filtrate, concentrating under reduced pressure to remove ethanol and water, and spray drying to obtain herba Portulacae extract.
The aloe vera extract is prepared by the following steps:
Taking mature aloe vera leaf with length not less than 40cm and widest part not less than 6cm, cleaning, removing leaf skin to obtain internal gelatinous mesophyll, stirring, crushing, squeezing juice, centrifuging at 5000rpm for 60min to obtain supernatant, and obtaining aloe vera extract.
The preparation method of the kuh-seng root extract comprises the following steps:
Pulverizing radix Sophorae Flavescentis into radix Sophorae Flavescentis powder, adding 5 times of water into radix Sophorae Flavescentis powder, extracting at 40deg.C for 15 hr, and filtering with 200 mesh filter cloth to obtain first residue and filtrate. Adding 8 times of water into the first filter residue, reflux extracting for 5h, filtering with 200 mesh filter cloth to obtain second filtrate. Mixing the first filtrate and the second filtrate, concentrating, and drying to obtain radix Sophorae Flavescentis extract.
The preparation method of the field mint leaf extract comprises the following steps:
Washing Tian Yebao folium Nelumbinis, pulverizing, adding 2 times of water into herba Menthae powder, reflux extracting for 3 hr, cooling, filtering, concentrating, and drying to obtain herba Menthae extract.
The preparation method of the golden chamomile extract comprises the following steps:
soaking flos Matricariae Chamomillae in 14 times of ethanol water solution for 1 hr, heating and keeping micro-boiling for 1 hr, filtering the extractive solution to obtain filtrate, concentrating the filtrate to relative density of 1.10, and spray drying to obtain flos Matricariae Chamomillae extract.
The preparation method of the moisturizing composition comprises the following specific steps:
and uniformly mixing the raw materials according to the mass percentage.
Example 2
A moisturizing composition comprises, by mass, 10% of inonotus obliquus extract, 15% of purslane extract, 5% of aloe vera extract, 3% of radix sophorae flavescentis extract, 9% of peppermint leaf extract, 12% of chamomile extract, 20% of butanediol, 12% of glycerol, 1, 2-hexanediol, 0.5% of p-hydroxyacetophenone and the balance of water.
The preparation method of the inonotus obliquus extract comprises the following steps:
(1) According to the inoculation amount of the inonotus obliquus strain as 10% of the total mass of a basic seed culture medium, inoculating the inonotus obliquus strain into the basic seed culture medium added with sodium caseinate and synephrine, wherein the concentration of the sodium caseinate and the synephrine in the basic seed culture medium is 0.15g/L of sodium caseinate and 5mg/L of synephrine, and culturing for 7d at 30 ℃ to obtain inonotus obliquus mycelium, and the nutrition composition of the basic seed culture medium is 20g/L of glucose, 5g/L of peptone, g/L of monopotassium phosphate and 1.5g/L of anhydrous magnesium sulfate.
(2) Inoculating Inonotus obliquus mycelium obtained in the step (1) into a fermentation medium added with shikimic acid and sodium copper chlorophyllin according to the inoculation amount of the Inonotus obliquus mycelium being 12% of the total mass of the fermentation medium, wherein the concentration of the shikimic acid and the sodium copper chlorophyllin in the fermentation medium is 15mg/L shikimic acid and 1mg/L sodium copper chlorophyllin, and continuously culturing for 10d at 28 ℃ to obtain a product, and the nutrition composition of the fermentation medium is 20g/L glucose, 6g/L beef extract, 1.5.5 g/L dipotassium phosphate and 1.5g/L, mnSO 4·H2 O0.15 g/L anhydrous magnesium sulfate.
(3) Crushing the product obtained in the step (2) to a granularity of 5mm, adding water, extracting under reflux for 3h to obtain an extracting solution, filtering and concentrating the extracting solution, adding ethanol to precipitate, collecting the precipitate, and drying the precipitate to obtain the inonotus obliquus extract.
The preparation process of purslane extract, aloe vera extract, kuh-seng root extract, peppermint leaf extract and golden chamomile extract is the same as in example 1.
The preparation method of the moisturizing composition comprises the following specific steps:
and uniformly mixing the raw materials according to the mass percentage.
Example 3
A moisturizing composition comprises, by mass, 5% of inonotus obliquus extract, 6% of purslane extract, 3% of aloe vera extract, 1% of kuh-seng extract, 7% of Tian Yebao lotus leaf extract, 10% of golden chamomile extract, 15% of butanediol, 8% of glycerol, 0.5% of 1, 2-hexanediol, 0.3% of p-hydroxyacetophenone and the balance of water.
The preparation method of the inonotus obliquus extract comprises the following steps:
(1) According to the inoculation amount of the inonotus obliquus strain as 8% of the total mass of a basic seed culture medium, inoculating the inonotus obliquus strain into the basic seed culture medium added with sodium caseinate and synephrine, wherein the concentration of the sodium caseinate and the synephrine in the basic seed culture medium is 0.08g/L of sodium caseinate and 1mg/L of synephrine, and culturing for 15d at 20 ℃ to obtain inonotus obliquus mycelium, and the nutrition composition of the basic seed culture medium is 15g/L of glucose, 1g/L of peptone, g/L of monopotassium phosphate and 0.5g/L of anhydrous magnesium sulfate.
(2) Inoculating Inonotus obliquus mycelium obtained in the step (1) into a fermentation medium added with shikimic acid and sodium copper chlorophyllin according to the inoculation amount of the Inonotus obliquus mycelium being 8% of the total mass of the fermentation medium, wherein the concentration of the shikimic acid and the sodium copper chlorophyllin in the fermentation medium is 9mg/L shikimic acid and 0.4mg/L sodium copper chlorophyllin, and continuously culturing for 14d at 20 ℃ to obtain a product, and the nutrition composition of the fermentation medium is 15g/L glucose, 2g/L beef extract, g/L dipotassium phosphate, 0.5g/L, mnSO 4·H2 O0.05 g/L anhydrous magnesium sulfate.
(3) Crushing the product obtained in the step (2) to a granularity of 5mm, adding water, extracting for 2h under reflux to obtain an extracting solution, filtering and concentrating the extracting solution, adding ethanol to precipitate, collecting the precipitate, and drying the precipitate to obtain the inonotus obliquus extract.
The preparation process of purslane extract, aloe vera extract, kuh-seng root extract, peppermint leaf extract and golden chamomile extract is the same as in example 1.
The preparation method of the moisturizing composition comprises the following specific steps:
and uniformly mixing the raw materials according to the mass percentage.
Comparative example
Comparative example 1
Comparative example 1 differs from example 1 in that:
In step (1) of the preparation process of the inonotus obliquus extract, synephrine was not added to the basal seed medium, and the rest was the same as in example 1.
Comparative example 2
Comparative example 2 differs from example 1 in that:
in step (2) of the preparation process of the inonotus obliquus extract, shikimic acid is not added into the fermentation medium, and the rest is the same as in example 1.
Comparative example 3
Comparative example 3 differs from example 1 in that:
in the step (1) of the preparation process of the inonotus obliquus extract, shikimic acid is used in the basal seed medium to replace synephrine;
In step (2) of the Inonotus obliquus extract preparation process, synephrine was used in place of shikimic acid in the fermentation medium, the remainder being identical to example 1.
Test examples
Test example 1
Test example 1 polysaccharide content of Inonotus obliquus extracts prepared in examples 1-3 and comparative examples 1-3 was detected by phenol-sulfuric acid method, and the specific procedure is as follows:
10mg of the Inonotus obliquus extract prepared in examples 1-3 and comparative examples 1-3 was weighed and placed in a 50mL volumetric flask, and then dissolved, diluted and fixed to volume with ultrapure water until the volume was calibrated, and shaken uniformly to obtain an Inonotus obliquus extract sample solution.
2ML of each of the above sample solutions was taken, 2mL of a 5% phenol solution and 10mL of concentrated sulfuric acid were immediately added to each of the sample solutions, and mixed uniformly, and the mixture was subjected to constant temperature water bath at 80℃for 30 minutes, cooled to room temperature, and then absorbance of the samples was measured at 486nm wavelength, and each of the samples was measured three times, and the average was taken, and the polysaccharide content results in the Inonotus obliquus extract were shown in Table 1.
TABLE 1
As can be seen from the observation of Table 1, the polysaccharide content in the Inonotus obliquus extract prepared in examples 1-3 was higher, reaching more than 35.56 wt%.
The polysaccharide content of the Inonotus obliquus extract prepared in comparative examples 1-3 was reduced to a different extent than that of the Inonotus obliquus extract prepared in examples 1-3, wherein the polysaccharide content of the Inonotus obliquus extract prepared in comparative example 2 was the lowest, and was only 18.27wt%. In the process of the inonotus obliquus extract, synephrine is not added in the basic seed culture medium of the comparative example 1, shikimic acid is not added in the fermentation culture medium of the comparative example 2, and the culture medium of the comparative example 3 changes the adding time of the synephrine and the shikimic acid. From the above, in the process of the inonotus obliquus extract, the content of polysaccharide in the inonotus obliquus extract can be influenced by changing the usage amount and the adding time of synephrine and shikimic acid.
The effects of adding the moisturizing compositions of examples 1 to 3 and comparative examples 1 to 3 of the present invention will be described below.
The skin care product consists of the following raw materials in percentage by mass:
Carbomer 0.2%, PEG-40 hydrogenated castor oil 5%, behenyl alcohol 0.7%, each of the moisturizing compositions described above 3%, the balance being water.
Test example 2
Skin irritation of skin care products obtained by adding the moisturizing compositions of examples 1 to 3 and comparative examples 1 to 3 was detected by using 2015 "cosmetic safety technical Specification" as a reference standard, and the specific procedure is as follows:
Subjects were selected from 120 subjects aged 18-45 years, and randomized into 6 groups with a 1:1 ratio between men and women.
The detection method comprises the steps of putting 0.02g of skin care products obtained by adding the moisturizing compositions of examples 1-3 and comparative examples 1-3 into spot testers respectively, covering the spot testers added with the test substances on the curved sides of forearms of the testers by using a non-stimulated cloth-based adhesive tape, pressing the non-stimulated cloth-based adhesive tape by using palms to uniformly adhere the non-stimulated cloth-based adhesive tape to the surfaces of the forearms of the testers, continuing for 24 hours, removing the spot testers for 30 minutes, and observing skin reactions after the indentations disappear. If the result is negative, the observation is carried out again 48 hours after the patch test, and the skin irritation detection result after 48 hours is shown in Table 2.
TABLE 2
From an examination of Table 2, it is clear that the moisturizing composition provided by the present invention is mild and non-irritating to the skin and safe to use.
Test example 3
The moisturizing effect of the skin care products obtained by adding the moisturizing compositions of examples 1 to 3 and comparative examples 1 to 3 was examined with reference to QB/T4256-2011, evaluation guidelines for moisturizing efficacy of cosmetics, and the specific procedure is as follows:
Subjects were selected from 60 healthy female subjects aged 18-45 years, randomized into 6 groups.
The temperature is 22 ℃ and the humidity is 50% in the tested environment, so that direct sunlight is avoided.
Test method all subjects began the test 30 minutes after entering the test environment. The subjects applied the moisturizing compositions of examples 1-3 and comparative examples 1-3 on their faces once a day, each morning and evening, and the skin moisture content was measured before use and after use of the skin care product 28d, respectively, and each data set is an average of the test results of the panel.
Wherein, skin hydration content change rate (%) = (skin hydration content after use of skin care product 28 d-skin hydration content before use)/skin hydration content before use×100%, and the results are shown in table 3.
TABLE 3 Table 3
The higher the value of the change rate of the skin hydration content, the better the moisturizing effect of the skin care product is indicated. From an examination of Table 3, it is apparent that the composition of the present invention has an excellent moisturizing effect. Specifically, after the skin care product 28d added with the moisturizing composition of the present invention is used, the skin hydration content variation rate reaches more than 68.47%.
The moisturizing effect of the skin care products added with the moisturizing compositions of comparative examples 1-3 was reduced to a different extent than those of examples 1-3. The improvement of the polysaccharide content can promote the moisturizing effect of the skin care product by combining the result of the polysaccharide content in the inonotus obliquus extract.
Finally, the above embodiments are only for illustrating the technical solution of the present invention, and are not limited thereto. While the basic principles and main features of the present invention have been described above with specific embodiments, modifications or substitutions may be made thereto without departing from the spirit of the invention as claimed.
Claims (10)
1. A moisturizing composition is characterized by comprising, by mass, 5-10% of inonotus obliquus extract, 6-15% of purslane extract, 3-5% of aloe vera extract, 1-3% of radix sophorae flavescentis extract, 7-9% of field mint leaf extract, 10-12% of golden chamomile extract, 15-20% of butanediol, 8-12% of glycerol, 0.5-1% of 1, 2-hexanediol, 0.3-0.5% of p-hydroxyacetophenone and the balance of water;
the preparation process of the inonotus obliquus extract comprises the following steps:
(1) Inoculating the inonotus obliquus strain into a basic seed culture medium added with sodium caseinate and synephrine for culturing to obtain inonotus obliquus mycelium;
(2) Inoculating the inonotus obliquus mycelium obtained in the step (1) to a fermentation medium added with shikimic acid and sodium copper chlorophyllin for continuous culture to obtain a product;
(3) Crushing the product obtained in the step (2), adding water for reflux extraction to obtain an extracting solution, filtering and concentrating the extracting solution, adding ethanol for precipitation, collecting the precipitate, and drying the precipitate to obtain the inonotus obliquus extract.
2. The moisturizing composition according to claim 1, wherein the concentration of the sodium caseinate and the synephrine in the basic seed culture medium in the step (1) is 0.08-0.15g/L, 1-5mg/L, and the nutritional composition of the basic seed culture medium is 15-20g/L glucose, 1-5g/L peptone, 0.5-1.5g/L monopotassium phosphate and 0.5-1.5g/L anhydrous magnesium sulfate.
3. The moisturizing composition of claim 1, wherein the inoculated amount of the Inonotus obliquus strain in the step (1) is 8-10% of the total mass of the basic seed medium, the culture temperature is 20-30 ℃, and the culture time is 7-15d.
4. The moisturizing composition according to claim 1, wherein the concentration of shikimic acid and sodium copper chlorophyllin in the fermentation medium in the step (2) is 9-15mg/L, 0.4-1mg/L, and the nutrition composition of the fermentation medium is 15-20g/L glucose, 2-6g/L beef extract, 0.5-1.5g/L dipotassium hydrogen phosphate, 0.5-1.5g/L, mnSO 4·H2 O and 0.05-0.15g/L.
5. The moisturizing composition according to claim 1, wherein the inoculated amount of the inonotus obliquus mycelium in the step (2) is 8-12% of the total mass of the fermentation medium, and the continuous culture is carried out at a temperature of 20-28 ℃ for 10-14d.
6. The moisturizing composition of claim 1, wherein the time of the reflux extraction in step (3) is 2 to 3 hours.
7. A method of preparing a moisturizing composition according to any one of claims 1 to 6, comprising the steps of:
and uniformly mixing the raw materials according to the mass percentage.
8. Use of a moisturizing composition according to any one of claims 1 to 6 for the preparation of a skin care product.
9. Use of a moisturizing composition according to claim 8, wherein the moisturizing composition comprises 1-5% by mass of the total mass of the skin care product.
10. The use of the moisturizing composition according to claim 8, wherein the skin care product further comprises the following raw materials, by mass, 0.1-0.3% of carbomer, 4-6% of PEG-40 hydrogenated castor oil, 0.5-1% of behenyl alcohol and the balance of water.
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