CN118956538B - Contactless sperm intelligent screening instrument - Google Patents
Contactless sperm intelligent screening instrument Download PDFInfo
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- CN118956538B CN118956538B CN202411040747.XA CN202411040747A CN118956538B CN 118956538 B CN118956538 B CN 118956538B CN 202411040747 A CN202411040747 A CN 202411040747A CN 118956538 B CN118956538 B CN 118956538B
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Abstract
本发明提供了一种无接触式精子智能筛选仪，涉及精子筛选技术领域，其包括筛选箱体、破碎组件、分流组件、盛液器皿以及显微镜；筛选箱体包括下箱体、盖体以及锥座；锥座的中部开设有处理腔，破碎组件设置于锥座内，用于破碎分解睾丸组织；盛液器皿设置于下箱体上，分流组件位于盛液器皿与破碎组件之间，分流组件用于将经剪切破碎处理后的含有精子的溶液分流至盛液器皿内；显微镜安装于盖体上，且位于盛液器皿的顶部；盖体内壁安装有紫外线除菌灯，下箱体内设置有空气循环净化扇。本发明将传统的精子筛选的多个步骤整合在一个仪器中，方便操作和观测，全程无菌操作，减少了精子被污染的可能性，保障精子活性。
The present invention provides a non-contact sperm intelligent screening instrument, which relates to the technical field of sperm screening, and includes a screening box, a crushing component, a diversion component, a liquid container and a microscope; the screening box includes a lower box, a cover and a cone seat; a processing chamber is provided in the middle of the cone seat, and the crushing component is arranged in the cone seat for crushing and decomposing testicular tissue; the liquid container is arranged on the lower box, and the diversion component is located between the liquid container and the crushing component, and the diversion component is used to divert the solution containing sperm after shearing and crushing treatment to the liquid container; the microscope is installed on the cover and is located on the top of the liquid container; an ultraviolet sterilization lamp is installed on the inner wall of the cover, and an air circulation purification fan is arranged in the lower box. The present invention integrates multiple steps of traditional sperm screening into one instrument, which is convenient for operation and observation, and sterile operation is performed throughout the process, which reduces the possibility of sperm contamination and ensures sperm activity.
Description
Technical Field
The invention relates to the technical field of sperm screening, in particular to a non-contact intelligent sperm screening instrument.
Background
Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technology that is part of the in vitro fertilization cycle. In vitro screening of ICSI sperm is very important and directly affects the transmission of information from the male parent to the pre-implantation embryo, the most common method for ICSI sperm selection being microscopic assessment of sperm motility and morphology.
The existing sperm screening mostly adopts a mode of directly observing under a microscope, utilizes a convex lens principle, amplifies a sperm structure and judges sperm activity according to a sperm movement distance by planning a vessel line, has a simple structure, is only used for seeing the sperm structure and the movement process thereof, is easy to pollute sperm in the screening operation process, ensures that the observed sperm cannot be used for fertilization any more, and lacks a non-contact sterile sperm detection screening instrument.
Disclosure of Invention
In order to solve the technical problems, the invention provides the non-contact intelligent sperm screening instrument, which integrates a plurality of steps of shearing and crushing, filtering, liquid separation, observation screening and the like in the traditional sperm screening operation into one instrument, and finally, the target sperm can be taken out, so that the operation and observation are convenient, the whole-process sterile operation is realized, the possibility of sperm pollution is reduced, and the sperm activity is ensured.
In order to achieve the aim, the invention provides a non-contact intelligent sperm screening instrument which comprises a screening box body, a crushing assembly, a shunting assembly, a liquid container and a microscope;
The screening box body comprises a lower box body, a cover body and a conical seat arranged on the top wall of the cover body, wherein a processing cavity is formed in the middle of the conical seat, and the upper end and the lower end of the processing cavity respectively penetrate through the conical seat and the cover body;
the crushing assembly is arranged in the conical seat and is used for crushing and decomposing testis tissues;
The liquid container is arranged on the lower box body, the flow distribution assembly is positioned between the liquid container and the crushing assembly, and the flow distribution assembly is used for distributing the solution containing sperms after shearing and crushing treatment into the liquid container;
the microscope is arranged on the cover body and is positioned at the top of the liquid container;
The ultraviolet sterilizing lamp is arranged on the inner wall of the cover body, and the air circulation purifying fan is arranged in the lower box body.
Further, broken subassembly is including handling the stick and handling the mortar of stick adaptation with handling, the preceding terminal surface that handles the stick and be close to handling the mortar outwards protrudes and is formed with hemispherical and cuts the sphere, it cuts the sphere to handle mortar up end indent formation, it has two-way barb thorns to go up to cut the sphere and cut the equal dense distribution on the sphere down.
Further, a plurality of two-way barb thorns of upper shearing sphere are the spiral arrangement in circumference, a plurality of two-way barb thorns of lower shearing sphere are even densely arranged.
Further, the processing rod is sleeved with a check ring, the processing rod is abutted with the top wall of the conical seat through the check ring, and the position of the check ring in the axial direction of the processing rod is adjustable.
Further, handle the mortar and include sleeve pipe and indent hemisphere body, hemisphere body is located the intraductal one end that just is close to the processing stick of sleeve pipe, hemisphere body's outer border and sleeve pipe outer border fixed connection and be formed with the water conservancy diversion inclined plane, a plurality of feed liquor holes have been seted up on the water conservancy diversion inclined plane.
Further, be provided with the filter screen in the conical seat, the filter screen is located the bottom of handling the mortar, the filter screen is used for filtering testicle tissue broken bits.
Further, a rotary platform is arranged in the lower box body, the bottom of the rotary platform is connected with a driving motor, the motor is fixed in the lower box body, a plurality of limiting holes for placing liquid containing vessels are formed in the rotary platform, and a plurality of limiting holes are uniformly distributed along the circumferential direction of the rotary platform;
The shunt assembly comprises a receiving disc and a plurality of liquid dividing pipes, the receiving disc is positioned at the bottom of the crushing assembly, the liquid dividing pipes are in one-to-one correspondence with the liquid containing vessels on the limiting holes, one ends of the liquid dividing pipes are communicated with the inner cavities of the receiving disc, the other ends of the liquid dividing pipes are positioned at the tops of the liquid containing vessels, and each liquid dividing pipe is provided with an electromagnetic control valve for controlling the opening and closing of the liquid dividing pipe.
Further, the microscope is connected with a computer, and can transmit the observation picture to the computer, and analysis and judgment are carried out by using sperm state analysis software on the computer.
Further, a picking and placing port is formed in the top wall of the cover body, the picking and placing port is located at the top of one of the limiting holes on the rotary platform, a sliding cover capable of sealing the picking and placing port is arranged at the picking and placing port, and a separation groove for placing the sliding cover is formed in the inner wall of the caliber of the picking and placing port and is communicated with the picking and placing port;
The lifting cylinder is arranged in the lower box body and is positioned right below the taking and placing opening, a piston rod of the lifting cylinder is connected with a sucker, and the piston rod of the lifting cylinder can drive the liquid container at the corresponding position to move in the vertical direction through the sucker.
Further, one side of the screening box body is provided with a central control device, and the lifting cylinder and the driving motor are connected with the central control device.
The invention has the beneficial effects that:
The non-contact intelligent sperm screening instrument can provide a sterile environment, integrates multiple steps of shearing and crushing, filtering, liquid separation, observation screening and the like in the traditional sperm screening operation into one instrument, and finally can take out target sperms, is convenient to operate and observe, performs aseptic operation in the whole process, reduces the possibility of sperm pollution and ensures sperm activity.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a partial cross-sectional view showing the internal structure of the screening box according to the present invention.
Fig. 3 is a cross-sectional view showing the internal structure of the screening box according to the present invention.
Fig. 4 is a schematic view of a part of the structure of the crushing assembly according to the invention.
Fig. 5 is a schematic view of the structure of the bi-directional barb of the present invention.
The device comprises a lower box body, a rotary platform, a limiting hole, a driving motor, a driving fan, a circulating air purifying fan, a cover body, a collecting opening, a suction cup, a observing position, a sliding cover, a separating groove, a lifting cylinder, a suction cup, a conical seat, a ultraviolet degerming lamp, a 4-breaking assembly, a 41-processing rod, a 411-upper shearing spherical surface, a 412-two-way barb hook, a 42-retaining ring, a 43-processing mortar, a 431-casing, a 432-hemispherical body, a 433-lower shearing spherical surface, a 434-guiding inclined surface, a 435-liquid inlet, a 44-filtering net, a 5-splitting assembly, a 51-receiving tray, a 52-liquid separating dropper, a 6-liquid containing vessel, a 7-microscope and a 8-central control device.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings. The drawings are simplified schematic representations which merely illustrate the basic structure of the invention and therefore show only the structures which are relevant to the invention.
The invention discloses a contactless intelligent sperm screening instrument.
Referring to fig. 1 and 2, a non-contact intelligent sperm screening instrument comprises a screening box body, a crushing assembly 4, a flow distribution assembly 5, a liquid container 6 and a microscope 7, wherein the screening box body comprises a lower box body 1, a cover body 2 and a conical seat 3 arranged on the top wall of the cover body 2, a processing cavity is formed in the middle of the conical seat 3, and the upper end and the lower end of the processing cavity respectively penetrate through the conical seat 3 and the cover body 2. The cover body 2 is provided with an observation position 22, the microscope 7 is arranged at the observation position 22, and the lower box body 1 is provided with an observation light source corresponding to the observation position for observation imaging of the microscope 7.
Referring to fig. 2 and 3, a crushing assembly 4 is arranged in a cone seat 3 and is used for crushing and decomposing testis tissues, a liquid containing vessel 6 is arranged on a lower box body 1, a flow dividing assembly 5 is arranged between the liquid containing vessel 6 and the crushing assembly 4 and is used for dividing a solution containing sperms after shearing and crushing treatment into the liquid containing vessel 6, an ultraviolet degerming lamp 31 is arranged on the inner wall of a cover body 2, an air circulation purifying fan 13 is arranged in the lower shell, and multi-layer filter cotton is arranged on the inner side of the air circulation purifying fan, so that sterilization and dust removal of air in an instrument are realized, and sperm pollution is prevented.
The microscope 7 is arranged on the cover body 2 and positioned at the top of the liquid container 6 and used for observing the sperm motility state and the like, in addition, the microscope 7 is connected with a computer, and can transmit the observation picture to the computer, and the sperm motility is judged by utilizing the sperm motility analysis software in the computer in an auxiliary way. It should be noted that, the related art has been disclosed in the present patent publication CN117911916A, CN117409406A, CN117409406a, which is related to the sperm status analysis software of the present application, and is not the focus of protection of the present application, so that no detailed description is given.
In application, the extracted testis tissue is placed in an instrument, ground, decomposed and filtered by a breaking component 4, and by adding a reagent for detection (such as 2ml, G-MOPS containing 5% HAS), sperm flows into a liquid container 6 through a splitting component 5, is observed by a microscope 7, and the observation picture or video is transmitted to a computer. The instrument integrates a plurality of steps of the traditional sperm screening operation into one instrument, is convenient to operate and observe, is in whole-process sterile operation, and reduces the possibility of pollution.
Referring to fig. 3 to 4, in this embodiment, the crushing assembly 4 includes a treatment rod 41 and a treatment socket 43 adapted to the treatment rod 41, wherein a hemispherical upper shearing spherical surface 411 is formed by the treatment rod 41 protruding outwards near the front end surface of the treatment socket 43, a lower shearing spherical surface 433 is formed by the upper end surface of the treatment socket 43 concave inwards, and two-way barbed needles are densely distributed on the upper shearing spherical surface 411 and the lower shearing spherical surface 433, wherein a plurality of two-way barbed needles of the upper shearing spherical surface 411 are spirally arranged in circumferential direction, and a plurality of two-way barbed needles of the lower shearing spherical surface 433 are uniformly densely distributed. The surface to be treated adopts a spherical surface to effectively increase the area of the surface to be treated and ensure the shearing and crushing effect, and the upper shearing spherical surface 411 is spirally arranged with two-way barb thorns, and the testicle tissue can be torn to the edge or the center of the cambered surface by rotating the treating rod 41 in different directions, so that the testicle tissue can be conveniently torn.
Referring to fig. 4, a retainer ring 42 is sleeved on the processing rod 41, the processing rod 41 is abutted with the top wall of the conical seat 3 through the retainer ring 42, the position of the retainer ring 42 in the axial direction of the processing rod 41 can be adjusted, and then the gap between the upper shearing spherical surface 411 and the lower shearing spherical surface 433 can be changed, so that the effect of adjusting the size of the grinding tissue is achieved. In this embodiment, positioning rings may be disposed on two sides of the retainer ring 42, and the positioning rings are disposed on the treatment rod 41 and are in threaded connection with the treatment rod 41, so that the adjustment of the position of the retainer ring 42 is achieved by rotating the positioning rings on two sides of the retainer ring 42.
The treatment mortar 43 comprises a sleeve 431 and a concave hemispherical body 432, the hemispherical body 432 is positioned in the sleeve 431 and is close to one end of the treatment rod 41, the outer edge of the hemispherical body 432 is fixedly connected with the outer edge of the sleeve 431, a diversion inclined plane 434 is formed, and a plurality of liquid inlets 435 are formed in the diversion inclined plane 434. The diversion ramp 434 is configured so that sperm can slowly flow down the diversion ramp 434 and into the vessel 6 through the inlet 435 when rinse liquid is added.
In this embodiment, a filter screen 44 is further installed in the cone seat 3, the filter screen 44 is located at the bottom of the treatment mortar 43, and the filter screen 44 is used for filtering testicle tissue residues.
Referring to fig. 3, a rotary platform 11 is disposed in the lower case 1, a driving motor 12 is connected to the bottom of the rotary platform 11, the motor is fixed in the lower case 1, a plurality of limiting holes 111 for placing the liquid container 6 are formed in the rotary platform 11, 12 limiting holes 111 in this embodiment are disposed, and the limiting holes 111 are uniformly distributed along the circumferential direction of the rotary platform 11. The flow dividing assembly 5 comprises a receiving disc 51 and a plurality of liquid dividing pipes 52, the receiving disc 51 is positioned at the bottom of the crushing assembly 4, the liquid dividing pipes 52 are in one-to-one correspondence with the liquid containing vessels 6 on the limiting holes 111, one ends of the liquid dividing pipes 52 are communicated with the inner cavities of the receiving disc 51, the other ends of the liquid dividing pipes 52 are positioned at the top of the liquid containing vessels 6, and electromagnetic control valves are arranged on the liquid dividing pipes 52 to control the opening and closing of the liquid dividing pipes 52. Through this reposition of redundant personnel subassembly 5 can be to each flourishing liquid household utensils 6 add the appearance simultaneously or to the sample in the flourishing liquid household utensils 6 of certain alone, satisfy different screening operation demands.
Referring to fig. 1 to 3, a picking and placing opening 21 is formed in the top wall of the cover body 2, the picking and placing opening 21 is located at the top of one of the limiting holes 111 on the rotary platform 11, a sliding cover 23 capable of sealing the picking and placing opening is arranged at the picking and placing opening 21, a separation groove 24 for placing the sliding cover 23 is formed in the caliber inner wall of the picking and placing opening 21, the separation groove 24 is communicated with the picking and placing opening 21, and the picking and placing opening 21 is opened and closed by sliding the sliding cover 23.
The lifting cylinder 25 is arranged in the lower box body 1, the lifting cylinder 25 is positioned under the taking and placing port 21, a piston rod of the lifting cylinder 25 is connected with a sucker 26, and the piston rod of the lifting cylinder 25 can drive the liquid container 6 at the corresponding position to move in the vertical direction through the sucker 26.
One side of the screening box body is provided with a central control device 8, and a lifting cylinder 25 and a driving motor 12 are connected with the central control device 8. The central control device 8 is provided with corresponding keys, the driving motor 12 rotates the corresponding liquid containing vessel 6 to the lower part of the take-out port 21 by pressing the keys, and the lifting cylinder 25 operates to lift the liquid containing vessel 6 to the outside of the take-out port 21, so that the liquid containing vessel 6 can be taken out, and the operation is simple and convenient.
The working principle of the non-contact intelligent sperm screening instrument is that when the intelligent sperm screening instrument is used, extracted testis tissues are placed into the instrument, after the position of a retainer ring 42 is adjusted, a processing rod 41 is inserted into a conical seat 3, the processing rod 41 is rotated to cut, crush and decompose the testis tissues, then a reagent for inspection is added, sperms flow into a liquid containing vessel 6 through a flow dividing component 5, a rotating platform 11 is rotated to rotate sperms in different liquid containing vessels 6 to an observation position 22, then a microscope 7 is used for observation, observation pictures or videos and the like are transmitted to a computer, the activity and the like of the sperms are analyzed by software in the computer, and according to analysis results, an operator lifts the sperms in the needed liquid containing vessel 6 through keys on a central control device 8. The whole instrument integrates a plurality of steps of the traditional sperm screening operation into one instrument, is convenient to operate and observe, is in whole sterile operation, and reduces the possibility of pollution.
With the above-described preferred embodiments according to the present invention as an illustration, the above-described descriptions can be used by persons skilled in the relevant art to make various changes and modifications without departing from the scope of the technical idea of the present invention. The technical scope of the present invention is not limited to the description, but must be determined according to the scope of claims.
Claims (6)
1. The non-contact intelligent sperm screening instrument is characterized by comprising a screening box body, a crushing assembly (4), a flow dividing assembly (5), a liquid container (6) and a microscope (7);
The screening box body comprises a lower box body (1), a cover body (2) and a conical seat (3) arranged on the top wall of the cover body (2), wherein a processing cavity is formed in the middle of the conical seat (3), and the upper end and the lower end of the processing cavity respectively penetrate through the conical seat (3) and the cover body (2);
the crushing assembly (4) is arranged in the conical seat (3) and is used for crushing and decomposing testis tissues;
the liquid container (6) is arranged on the lower box body (1), the flow dividing assembly (5) is positioned between the liquid container (6) and the crushing assembly (4), and the flow dividing assembly (5) is used for dividing the solution containing sperms after shearing and crushing treatment into the liquid container (6);
the microscope (7) is arranged on the cover body (2) and is positioned at the top of the liquid container (6);
An ultraviolet sterilizing lamp (31) is arranged on the inner wall of the cover body (2), and an air circulation purifying fan (13) is arranged in the lower box body (1);
The crushing assembly (4) comprises a treatment rod (41) and a treatment mortar (43) matched with the treatment rod (41), wherein the front end surface of the treatment rod (41) close to the treatment mortar (43) protrudes outwards to form a hemispherical upper shearing spherical surface (411), the upper end surface of the treatment mortar (43) is concavely provided with a lower shearing spherical surface (433), and two-way barb thorns are densely distributed on the upper shearing spherical surface (411) and the lower shearing spherical surface (433);
the two-way barbed needles of the upper shearing spherical surface (411) are spirally arranged in the circumferential direction, and the two-way barbed needles of the lower shearing spherical surface (433) are uniformly and densely arranged;
A check ring (42) is sleeved on the treatment rod (41), the treatment rod (41) is abutted with the top wall of the conical seat (3) through the check ring (42), and the position of the check ring (42) in the axial direction of the treatment rod (41) can be adjusted;
A rotary platform (11) is arranged in the lower box body (1), a driving motor (12) is connected to the bottom of the rotary platform (11), the motor is fixed in the lower box body (1), a plurality of limiting holes (111) for placing the liquid containing vessel (6) are formed in the rotary platform (11), and the limiting holes (111) are uniformly distributed along the circumferential direction of the rotary platform (11);
The shunt assembly (5) comprises a receiving disc (51) and a plurality of liquid dividing pipes (52), the receiving disc (51) is located at the bottom of the crushing assembly (4), the liquid dividing pipes (52) are in one-to-one correspondence with the liquid containing vessels (6) on the limiting holes (111), one ends of the liquid dividing pipes (52) are communicated with the inner cavities of the receiving disc (51), the other ends of the liquid dividing pipes are located at the tops of the liquid containing vessels (6), and electromagnetic control valves are arranged on the liquid dividing pipes (52) and used for controlling the opening and closing of the liquid dividing pipes (52).
2. The intelligent contact-free sperm screening instrument of claim 1, wherein the treatment mortar (43) comprises a sleeve (431) and a concave hemispherical body (432), the hemispherical body (432) is positioned in the sleeve (431) and is close to one end of the treatment rod (41), the outer edge of the hemispherical body (432) is fixedly connected with the outer edge of the sleeve (431) and is provided with a diversion inclined plane (434), and the diversion inclined plane (434) is provided with a plurality of liquid inlets (435).
3. The intelligent contactless sperm screening instrument of claim 2, wherein a filter screen (44) is arranged in the conical seat (3), the filter screen (44) is positioned at the bottom of the treatment mortar (43), and the filter screen (44) is used for filtering testis tissue slag.
4. The intelligent contactless sperm screening instrument of claim 3, wherein the microscope (7) is connected with a computer, and the microscope (7) can transmit the observation picture to the computer, and the analysis and judgment are performed by using sperm state analysis software on the computer.
5. The intelligent contactless sperm screening instrument of claim 4, wherein the top wall of the cover body (2) is provided with a picking and placing port (21), the picking and placing port (21) is positioned at the top of one of limit holes (111) on the rotary platform (11), the picking and placing port (21) is provided with a sliding cover (23) capable of sealing the picking and placing port, the caliber inner wall of the picking and placing port (21) is provided with a separation groove (24) for placing the sliding cover (23), and the separation groove (24) is communicated with the picking and placing port (21);
The lifting air cylinder (25) is arranged in the lower box body (1), the lifting air cylinder (25) is located under the taking and placing opening (21), a piston rod of the lifting air cylinder (25) is connected with a sucker (26), and the piston rod of the lifting air cylinder (25) can drive the liquid container (6) at the corresponding position to move in the vertical direction through the sucker (26).
6. The intelligent contactless sperm screening instrument of claim 5, wherein a central control device (8) is arranged on one side of the screening box body, and the lifting cylinder (25) and the driving motor (12) are connected with the central control device (8).
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112997061A (en) * | 2018-06-01 | 2021-06-18 | 艾斯图基因组学公司 | Method and apparatus for processing tissue samples |
| CN117917470A (en) * | 2023-04-19 | 2024-04-23 | 昆山嘉华自动化设备有限公司 | Tissue dissociation device |
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| KR101359693B1 (en) * | 2012-01-09 | 2014-02-10 | (주) 메디컬그룹베스티안 | Cell harvesting apparatus for cell processing and delivery system |
| CN114752498A (en) * | 2022-03-29 | 2022-07-15 | 北京寻因生物科技有限公司 | Dissociation module and dissociation device |
| CN115948229B (en) * | 2023-03-15 | 2023-05-30 | 中国医学科学院基础医学研究所 | A tissue dissociation device for obtaining living cells |
| CN116731786A (en) * | 2023-07-05 | 2023-09-12 | 海口卜咎五金机电有限公司 | Plant essence extraction element |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112997061A (en) * | 2018-06-01 | 2021-06-18 | 艾斯图基因组学公司 | Method and apparatus for processing tissue samples |
| CN117917470A (en) * | 2023-04-19 | 2024-04-23 | 昆山嘉华自动化设备有限公司 | Tissue dissociation device |
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