CN118883978B - Full-automatic detection device of rice fermentation fungus acid - Google Patents
Full-automatic detection device of rice fermentation fungus acid Download PDFInfo
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- CN118883978B CN118883978B CN202411391806.8A CN202411391806A CN118883978B CN 118883978 B CN118883978 B CN 118883978B CN 202411391806 A CN202411391806 A CN 202411391806A CN 118883978 B CN118883978 B CN 118883978B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
- G01N35/1083—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with one horizontal degree of freedom
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4022—Concentrating samples by thermal techniques; Phase changes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
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Abstract
本发明公开了一种米酵菌酸的全自动检测装置，包括底座，所述底座的顶端两侧均通过螺栓固定连接有支撑板，左侧所述支撑板的内侧顶部焊接有连接板，所述连接板的内部放置有若干组样本储存罐，所述样本储存罐的出料端设置有第一计量阀，所述底座的顶端左侧安装有试管架，所述试管架内均匀放置有是若干组离心管。本发明通过转移机构、加料机构、吸取机构、浓缩机构和检测机构的配合使用，实现全自动对不同的样本进行检测，无需工作人员操作，方便快捷，能保证检测的连续性，每一个样本的检测不共用离心管以及移液枪，保证了检测数据的准确性，且通过试剂检测的方式，检测成本较低，进一步满足了工作人员的需求，有利于推广使用。
The present invention discloses a fully automatic detection device of fumonisin, including a base, both sides of the top of the base are fixedly connected with a support plate by bolts, a connecting plate is welded on the inner top of the left support plate, several groups of sample storage tanks are placed inside the connecting plate, and the discharge end of the sample storage tank is provided with a first metering valve, and a test tube rack is installed on the left side of the top of the base, and several groups of centrifuge tubes are evenly placed in the test tube rack. The present invention realizes fully automatic detection of different samples through the coordinated use of a transfer mechanism, a feeding mechanism, an absorption mechanism, a concentration mechanism and a detection mechanism, and does not require staff operation, which is convenient and fast, can ensure the continuity of detection, and the detection of each sample does not share a centrifuge tube and a liquid transfer gun, which ensures the accuracy of the detection data, and through the reagent detection method, the detection cost is low, further meets the needs of the staff, and is conducive to promotion and use.
Description
Technical Field
The invention relates to the technical field of rice fermentation acid detection, in particular to a full-automatic detection device for rice fermentation acid.
Background
The rice fermentation mycolic acid is a fat-soluble acidic compound, the molecular formula is C 28H38O7, the relative molecular weight is 486.6, the rice fermentation mycolic acid is one of the coconut toxin pseudomonas rice subspecies metabolites, the rice fermentation mycolic acid exists in rice and flour products, edible fungi, tremella and other foods which are deteriorated or foamed for a long time, the toxicity is strong, the poisoning symptoms such as nausea, vomiting, convulsion and shock can occur for eaters, liver and brain nerve cells and kidney tissues are damaged, the mortality rate is up to 40% -100%, and a plurality of food poisoning events are caused in China, and strict precaution is needed.
Most of the existing methods for analyzing the milbemycetin adopt liquid chromatography or liquid chromatography-tandem mass spectrometry, but the detection mode needs to purchase expensive equipment, and has high cost. The rapid detection methods such as the colloidal gold immunoassay method and the time-resolved immunofluorescence analysis method are flexible, convenient and rapid, but the detection process has more steps, more reagents are needed to be added, the addition of the reagents often needs manual operation by staff, if the operation is improper, the detection card is easy to not develop color, and errors exist in the detection result, so that the detection requirement is difficult to meet.
Disclosure of Invention
In order to solve the technical problems, the full-automatic detection device for the milbemycetin is provided, and the technical scheme solves the problems that the existing milbemycetin detection in the background technology is more in operation steps in experiments, more reagents are needed to be added, the reagents are added to be manually operated by workers, the manual operation cost is higher, more uncontrollable elements exist, the detection card is not developed, and the detection result has poor precision.
In order to achieve the above purpose, the invention adopts the following technical scheme:
The utility model provides a full-automatic detection device of rice ferment fungus acid, includes the base, the top both sides of base all are through bolt fixedly connected with backup pad, the left side the inboard top welding of backup pad has the connecting plate, a plurality of groups sample holding vessel have been placed to the inside of connecting plate, the discharge end of sample holding vessel is provided with first metering valve, the test-tube rack is installed in the top left side of base, evenly placed in the test-tube rack and be a plurality of groups centrifuging tube, the centrifuging tube top is provided with the end cap, charging mechanism is installed on the test-tube rack right side, just base top middle part is provided with centrifuge and receipts useless case, the inside a plurality of groups of holes of placing that are provided with of centrifuge, base top right side is provided with concentration mechanism, mount, vortex concussion appearance top is provided with flat gasket, be provided with the storage cylinder on the mount, be provided with the second metering valve on the storage cylinder discharge end, the inside stores rice ferment fungus acid complex solution, the right side be provided with transfer mechanism between the inboard of backup pad and the connecting plate, the base top front side is installed and is received the inboard and is still provided with detection mechanism.
Preferably, the feeding mechanism comprises a fourth screw rod and an L-shaped plate, a mounting frame is mounted on the left side of the top end of the base, the fourth screw rod is rotationally connected to the inside of the mounting frame, the L-shaped plate is in threaded connection with the outer surface of the fourth screw rod, a fourth guide rod is fixedly connected to the inside of the mounting frame, the L-shaped plate is slidably connected to the fourth guide rod, an electric push rod is fixedly mounted on the top of the L-shaped plate, the output end of the electric push rod is fixedly connected with the mounting plate, two groups of storage barrels are mounted on the outer side of the mounting plate, a third metering valve is arranged on the discharge end of the storage barrels, one end of the fourth screw rod is fixedly connected to the output end of a fourth stepping motor, and the fourth stepping motor is mounted on the outer side of the mounting frame.
Preferably, the transfer mechanism comprises a first guide rod, the right side rotate between backup pad and the connecting plate and be connected with first lead screw, the both ends of first guide rod respectively with the inboard welding of right side backup pad and the inboard welding of connecting plate, first guide rod surface sliding connection has the movable plate, movable plate and first lead screw threaded connection, the right side the outside of backup pad is provided with first step motor, the output of first step motor and the right-hand member fixed connection of first lead screw, just the top of movable plate is provided with two sets of first cylinders, be provided with the connecting frame under the movable plate, connecting frame top fixed connection is at the output of first cylinder.
Preferably, the inside rotation of connecting frame is connected with the second lead screw, second lead screw surface threaded connection has the movable block, movable block sliding connection is on the second guide arm, just second guide arm fixed connection is in the inside of connecting frame, the connecting frame outside is provided with the second step motor, the second step motor output extends to the connecting frame inside to with the one end fixed connection of second lead screw, the inside rotation of movable block is connected with the rotary drum, driven gear is installed at rotary drum surface top, just movable block bottom fixedly connected with driving motor, driving motor's output fixed mounting has the driving gear with driven gear meshing.
Preferably, the bottom welding of a rotating cylinder has the underframe, the inside rotation of underframe is connected with first screw rod, the inside first connecting rod that still fixedly connected with of underframe, the first connecting rod surface sliding connection has two sets of first holders, the screw thread that the first screw rod both ends were seted up revolves to opposite, and two sets of first holders respectively threaded connection in the both ends of first screw rod surface, the one end fixed connection of first screw rod is at the output of first servo motor, just first servo motor installs in the outside of underframe.
Preferably, the inside top of a rotating cylinder is provided with a third stepping motor, the inside of the rotating cylinder is rotationally connected with a third screw rod, the top of the third screw rod is fixedly connected with the output end of the third stepping motor, the outer surface of the third screw rod is in threaded connection with a lifting plate, the lifting plate is in sliding connection with the outer surface of a third guide rod, the third guide rod is fixedly arranged in the rotating cylinder, two groups of electric telescopic rods are arranged on the outer side of the lifting plate, and fixing blocks are fixedly arranged at the output ends of the electric telescopic rods.
Preferably, the two groups of fixing blocks are rotationally connected with a second screw rod through bearings, threads formed at two ends of the second screw rod are opposite in rotation direction, two groups of second clamping pieces are connected with two ends of the outer surface of the second screw rod in a threaded mode and are slidably connected to a second connecting rod, two ends of the second connecting rod are welded with two groups of inner sides of the fixing blocks respectively, a second servo motor is arranged on the outer side of each fixing block, and the output end of the second servo motor is fixedly connected with one end of each second screw rod.
Preferably, the suction mechanism comprises a placement seat, a fixing seat and a second clamping plate, wherein the placement seat and the fixing seat are arranged on the front side of the top end of the base, a plurality of groups of pipetting guns are uniformly placed in the placement seat, the tops of the pipetting guns are provided with piston heads, the two sides of the top end of the fixing seat are respectively provided with a second cylinder, the output ends of the second cylinders are fixedly connected with a first clamping plate, the front side and the rear side of the fixing seat are respectively provided with a third cylinder, the output ends of the third cylinders are fixedly connected with the bottom of the frame, the inside of the frame is rotationally connected with a third screw rod, threads formed at the two ends of the third screw rod are opposite in rotation direction, the second clamping plate is provided with two groups of threads and is respectively connected with the two ends of the outer surface of the third screw rod, a third connecting rod is fixedly arranged in the frame, the second clamping plate is in sliding connection with the third connecting rod, and the outer side of the frame is provided with a third servo motor, and one end of the third screw rod is fixedly connected with the output end of the third servo motor.
Preferably, the concentration mechanism comprises a sample concentration instrument, the sample concentration instrument is arranged on the right side of the top end of the base, a transmission motor is fixedly arranged at the back of the sample concentration instrument, a rotating piece is rotatably connected inside the sample concentration instrument, the bottom of the rotating piece is fixedly connected with the output end of the transmission motor, a fifth screw rod and a fifth guide rod are arranged inside the rotating piece, the fifth screw rod is rotatably connected with the rotating piece, the fifth guide rod is fixedly connected with the rotating piece, a lifting piece is in threaded connection with the outer surface of the fifth screw rod, the lifting piece is in sliding connection with the fifth guide rod, a heating device is arranged at the top of the lifting piece, the output end of the heating device is communicated with a plurality of groups of connecting pipes, a fifth stepping motor is arranged at the top of the rotating piece, and the top of the fifth screw rod is fixedly connected with the output end of the fifth stepping motor.
Preferably, the detection mechanism comprises a placing plate, the placing plate is fixedly installed on the right side at the bottom end of the inner side of the supporting plate, a plurality of groups of detection cards are placed on the top of the placing plate at equal intervals, two groups of installation blocks are welded at the middle part of the inner side of the supporting plate, a sixth screw rod is connected between the installation blocks in a rotating mode, a movable plate is connected with the outer surface of the sixth screw rod in a threaded mode, a camera is installed at the bottom of the movable plate, a sixth guide rod is installed between the installation blocks in two groups, the movable plate is connected with the outer surface of the sixth guide rod in a sliding mode, a sixth stepping motor is arranged on the outer side of the installation blocks, and one end of the sixth screw rod is fixedly connected with the output end of the sixth stepping motor.
Compared with the prior art, the invention provides a full-automatic detecting device for the rice fermentation acid, which has the following beneficial effects:
According to the invention, through the cooperation of the transfer mechanism, the feeding mechanism, the suction mechanism, the concentration mechanism and the detection mechanism, the detection of different samples is realized, manual operation of a worker is not needed, convenience and rapidness are ensured, the detection of each sample does not share a centrifuge tube and a pipette gun, the samples are not polluted with each other, the accuracy of detection data is ensured, the labor force of the worker is liberated by the way of automatically adding reagents, the detection efficiency is improved, the same set of transfer mechanism is used, the detection cost is reduced, and the device can realize the extraction and blocking of test tube plugs, facilitates the suction and the discharge of the pipette gun and is favorable for popularization and use.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic view of another view of the present invention;
FIG. 3 is a top view of the present invention;
FIG. 4 is a schematic diagram of a transfer mechanism according to the present invention;
FIG. 5 is a schematic view of a moving plate according to the present invention;
FIG. 6 is a schematic view showing the internal structure of the connecting frame according to the present invention;
FIG. 7 is a schematic view showing the internal structure of the bottom frame of the present invention;
FIG. 8 is a schematic view of a second clamping member according to the present invention;
FIG. 9 is a schematic view of the structure of the test tube rack of the present invention;
FIG. 10 is a schematic view of a charging mechanism according to the present invention;
FIG. 11 is a schematic structural view of a fixing base according to the present invention;
FIG. 12 is an enlarged schematic view of the structure of the portion B shown in FIG. 2 according to the present invention;
FIG. 13 is a schematic view of a concentrating mechanism according to the present invention;
FIG. 14 is a schematic view of the A-amp configuration of FIG. 1 according to the present invention;
FIG. 15 is an enlarged view of the structure of FIG. 2 according to the present invention;
FIG. 16 is a schematic diagram of the detection card according to the present invention.
The reference numerals in the figures are:
1. The device comprises a base, 101, a supporting plate, 102, a connecting plate, 103, a sample storage tank, 104, a first metering valve, 105, a test tube rack, 106, a centrifuge tube, 107, a plug, 108, a centrifuge, 109, a placing hole, 110, a waste collection box, 111, a fixing frame, 112, a storage cylinder, 113, a second metering valve, 114, a vortex oscillator, 115 and a water cooling box;
2. The device comprises a transfer mechanism, 201, a first screw rod, 202, a first guide rod, 203, a first stepping motor, 204, a moving plate, 205, a first cylinder, 206, a connecting frame, 207, a second screw rod, 208, a second guide rod, 209, a second stepping motor, 210, a moving block, 211, a rotating cylinder, 212, a driving motor, 213, a driving gear, 214, a driven gear, 215, a bottom frame, 216, a first screw rod, 217, a first connecting rod, 218, a first servo motor, 219, a first clamping piece, 220, a third screw rod, 221, a third guide rod, 222, a third stepping motor, 223, a lifting plate, 224, an electric telescopic rod, 225, a fixed block, 226, a second connecting rod, 227, a second screw rod, 228, a second servo motor, 229 and a second clamping piece;
3. The device comprises a feeding mechanism, 301, a mounting frame, 302, a fourth screw rod, 303, a fourth guide rod, 304, a fourth stepping motor, 305, an L-shaped plate, 306, an electric push rod, 307, a mounting plate, 308, a storage cylinder and 309, and a third metering valve;
4. Suction mechanism 401, placement seat 402, pipette, 403, piston head 404, fixing seat 405, second cylinder 406, first clamping plate 407, third cylinder 408, frame 409, third screw 410, third connecting rod 411, third servo motor 412, second clamping plate;
5. the device comprises a concentration mechanism, a sample concentration instrument, 502, a rotating part, 503, a transmission motor, 504, a fifth screw rod, 505, a fifth guide rod, 506, a fifth stepping motor, 507, a lifting part, 508, a heating device, 509 and a connecting pipe;
6. The device comprises a detection mechanism, a placing plate, 602, a detection card, 603, a mounting block, 604, a sixth screw rod, 605, a sixth guide rod, 606, a sixth stepping motor, 607, a movable plate, 608 and a camera.
Detailed Description
The following description is presented to enable one of ordinary skill in the art to make and use the invention. The preferred embodiments in the following description are by way of example only and other obvious variations will occur to those skilled in the art.
Examples
Referring to fig. 1-16, a full-automatic detecting device for milbemycetin comprises a base 1, the top both sides of the base 1 are fixedly connected with a supporting plate 101 through bolts, the inside top of a left supporting plate 101 is welded with a connecting plate 102, a plurality of groups of sample storage tanks 103 are placed inside the connecting plate 102, the discharge end of each sample storage tank 103 is provided with a first metering valve 104, a test tube rack 105 is installed on the left side of the top of the base 1, a plurality of groups of centrifugal tubes 106 are uniformly placed inside the test tube rack 105, a plug 107 is arranged at the top of each centrifugal tube 106, a feeding mechanism 3 is installed on the right side of the test tube rack 105, a centrifugal machine 108 and a waste collecting box 110 are arranged in the middle of the top of the base 1, a plurality of groups of placing holes 109 are formed in the centrifugal machine 108, a concentrating mechanism 5, a fixing frame 111, a vortex vibration instrument 114 and a water cooling box 115 are arranged on the right side of the top of the base 1, a storage cylinder 112 is arranged on the discharge end of each fixing frame 111, a second metering valve 113 is arranged inside the storage cylinder 112, milbemycetin complex solution is stored inside the storage cylinder, a transfer mechanism 2 is arranged between the inside the right side of the supporting plate 101 and the connecting plate 102, and the front side of the base 1 is provided with a detection mechanism 6, and a detection mechanism is arranged on the front side of the base 1 is arranged on the right side, and the detecting mechanism is arranged on the front side of the base 101.
Examples
Referring to fig. 10, the feeding mechanism 3 includes a fourth screw rod 302 and an L-shaped plate 305, a mounting frame 301 is mounted on the left side of the top end of the base 1, the fourth screw rod 302 is rotatably connected to the inside of the mounting frame 301, the L-shaped plate 305 is in threaded connection with the outer surface of the fourth screw rod 302, a fourth guide rod 303 is fixedly connected to the inside of the mounting frame 301, the L-shaped plate 305 is slidably connected to the fourth guide rod 303, an electric push rod 306 is fixedly mounted on the top of the L-shaped plate 305, an output end of the electric push rod 306 is fixedly connected to the mounting plate 307, two groups of storage barrels 308 are mounted on the outer side of the mounting plate 307, a third metering valve 309 is disposed on the discharge end of the storage barrels 308, one end of the fourth screw rod 302 is fixedly connected to the output end of the fourth stepping motor 304, and the fourth stepping motor 304 is mounted on the outer side of the mounting frame 301.
As will be appreciated by those skilled in the art, the two storage barrels 308 are provided with two groups, respectively storing the rice-fermenting acid reagent a and the rice-fermenting acid reagent B, and the output end of the fourth stepping motor 304 drives the fourth screw rod 302 to rotate, so that the L-shaped plate 305 slides reciprocally along the outer surface of the fourth guide rod 303, and the output end of the electric push rod 306 extends or contracts to drive the two groups of storage barrels 308 to move left and right, and as the two groups of storage barrels 308 are provided with the third metering valve 309, quantitative addition of the rice-fermenting acid reagent a and the rice-fermenting acid reagent B in the interior of each centrifuge tube 106 is realized.
Examples
Referring to fig. 4 and 5, the transfer mechanism 2 includes a first guide rod 202, a first screw rod 201 is rotatably connected between the right supporting plate 101 and the connecting plate 102, two ends of the first guide rod 202 are welded with an inner side of the right supporting plate 101 and an inner side of the connecting plate 102 respectively, an outer surface of the first guide rod 202 is slidably connected with a moving plate 204, the moving plate 204 is in threaded connection with the first screw rod 201, a first stepping motor 203 is disposed on an outer side of the right supporting plate 101, an output end of the first stepping motor 203 is fixedly connected with a right end of the first screw rod 201, two groups of first cylinders 205 are disposed at a top of the moving plate 204, a connecting frame 206 is disposed under the moving plate 204, and a top of the connecting frame 206 is fixedly connected with an output end of the first cylinders 205.
Referring to fig. 6 and 7, a second screw rod 207 is rotatably connected to the inside of the connection frame 206, a moving block 210 is screwed to the outer surface of the second screw rod 207, the moving block 210 is slidably connected to a second guide rod 208, the second guide rod 208 is fixedly connected to the inside of the connection frame 206, a second stepping motor 209 is disposed on the outside of the connection frame 206, an output end of the second stepping motor 209 extends into the inside of the connection frame 206 and is fixedly connected to one end of the second screw rod 207, a rotating cylinder 211 is rotatably connected to the inside of the moving block 210, a driven gear 214 is mounted on the top of the outer surface of the rotating cylinder 211, a driving motor 212 is fixedly connected to the bottom of the moving block 210, and a driving gear 213 meshed with the driven gear 214 is fixedly mounted at an output end of the driving motor 212.
Referring to fig. 7, a bottom frame 215 is welded at the bottom of the rotating cylinder 211, a first screw rod 216 is rotatably connected to the inside of the bottom frame 215, a first connecting rod 217 is fixedly connected to the inside of the bottom frame 215, two groups of first clamping members 219 are slidably connected to the outer surface of the first connecting rod 217, threads formed at two ends of the first screw rod 216 are opposite in rotation direction, the two groups of first clamping members 219 are respectively and threadedly connected to two ends of the outer surface of the first screw rod 216, one end of the first screw rod 216 is fixedly connected to the output end of the first servo motor 218, and the first servo motor 218 is mounted on the outer side of the bottom frame 215.
Referring to fig. 8, a third stepper motor 222 is installed at the top end of the inside of the rotating cylinder 211, a third screw rod 220 is rotatably connected to the inside of the rotating cylinder 211, the top of the third screw rod 220 is fixedly connected to the output end of the third stepper motor 222, a lifting plate 223 is screwed to the outer surface of the third screw rod 220, the lifting plate 223 is slidably connected to the outer surface of a third guide rod 221, the third guide rod 221 is fixedly installed inside the rotating cylinder 211, two groups of electric telescopic rods 224 are arranged on the outer side of the lifting plate 223, and fixing blocks 225 are fixedly installed at the output ends of the two groups of electric telescopic rods 224.
Referring to fig. 8, the two sets of fixing blocks 225 are rotatably connected with a second screw 227 through bearings, threads formed at two ends of the second screw 227 are opposite in rotation direction, two ends of an outer surface of the second screw 227 are respectively and slidably connected with a second clamping member 229, the two sets of second clamping members 229 are respectively and slidably connected to a second connecting rod 226, two ends of the second connecting rod 226 are respectively welded to inner sides of the two sets of fixing blocks 225, a second servo motor 228 is arranged on the outer side of one set of fixing blocks 225, and an output end of the second servo motor 228 is fixedly connected with one end of the second screw 227.
As will be appreciated by those skilled in the art, the output end of the first stepper motor 203 drives the first screw 201 to rotate, so that the moving plate 204 can horizontally reciprocate, thereby realizing the horizontal reciprocation of the two groups of the first clamping members 219 and the two groups of the second clamping members 229; the connecting frame 206 is driven to move up and down through the output end of the first air cylinder 205, so that two groups of first clamping pieces 219 and two groups of second clamping pieces 229 move up and down, the second screw rod 207 is driven to rotate through the output end of the second stepping motor 209, the moving block 210 can longitudinally reciprocate, two groups of first clamping pieces 219 and two groups of second clamping pieces 229 longitudinally reciprocate, the driven gear 213 is driven to rotate through the output end of the driving motor 212, the driven gear 214 and the rotating cylinder 211 integrally rotate, the two groups of first clamping pieces 219 and the two groups of second clamping pieces 229 rotate, the directions of the two groups of first clamping pieces 219 and the two groups of second clamping pieces 229 are changed, the first screw rod 216 is driven to rotate through the output end of the first servo motor 218, the two groups of first clamping pieces 219 are close to or far away from each other, the third screw rod 220 is driven to rotate through the output end of the third stepping motor 222, the two groups of second clamping pieces 229 are driven to vertically reciprocate, the distance between the second clamping pieces 229 and the first clamping pieces 219 is changed, and the first clamping pieces 219 and the second clamping pieces 229 are formed by extending or shrinking the output ends 224 between the two groups of second clamping pieces.
Examples
Referring to fig. 11 and 12, the suction mechanism 4 includes a placement seat 401, a fixing seat 404, and a second clamping plate 412, where the placement seat 401 and the fixing seat 404 are disposed on a front side of a top end of the base 1, a plurality of groups of pipette guns 402 are uniformly disposed in the placement seat 401, a piston head 403 is disposed on a top of the pipette gun 402, two sides of a top end of the fixing seat 404 are respectively provided with a second cylinder 405, an output end of the second cylinder 405 is fixedly connected with the first clamping plate 406, front and rear sides of the fixing seat 404 are respectively provided with a third cylinder 407, an output end of the third cylinder 407 is fixedly connected with a bottom of the frame 408, a third screw 409 is rotatably connected inside the frame 408, threads formed at two ends of the third screw 409 are opposite in rotation direction, the second clamping plate 412 is disposed with two groups of threads and is respectively connected with two ends of an outer surface of the third screw 409, a third connecting rod 410 is fixedly mounted inside the frame 408, the second clamping plate 412 is slidably connected with the third connecting rod 410, and one end of the third screw 409 is fixedly connected with an output end of the third servo motor 411.
It will be understood by those skilled in the art that the two groups of the first clamping plates 406 are moved closer to or away from each other by extending or contracting the output ends of the two groups of the second cylinders 405, the frame 408 is driven to move up and down by the output ends of the third cylinders 407, the two groups of the second clamping plates 412 are moved up and down, and the third screw 409 is driven to rotate by the output ends of the third servo motor 411, so that the two groups of the second clamping plates 412 are moved closer to or away from each other.
Examples
Referring to fig. 13, the concentrating mechanism 5 includes a sample concentrating device 501, the sample concentrating device 501 is disposed on the right side of the top end of the base 1, a transmission motor 503 is fixedly mounted at the back of the sample concentrating device 501, a rotating member 502 is rotatably connected inside the sample concentrating device 501, the bottom of the rotating member 502 is fixedly connected to the output end of the transmission motor 503, a fifth screw rod 504 and a fifth guide rod 505 are disposed inside the rotating member 502, the fifth screw rod 504 is rotatably connected to the rotating member 502, the fifth guide rod 505 is fixedly connected to the rotating member 502, a lifting member 507 is screwed to the outer surface of the fifth screw rod 504, the lifting member 507 is slidably connected to the fifth guide rod 505, a heating device 508 is disposed at the top of the lifting member 507, the output end of the heating device 508 is communicated with a plurality of groups of connecting pipes 509, a fifth stepping motor 506 is disposed at the top of the rotating member 502, and the top of the fifth screw rod 504 is fixedly connected to the output end of the fifth stepping motor 506.
It can be understood by those skilled in the art that the output end of the transmission motor 503 drives the rotating member 502 to rotate, so that the lifting member 507 and the connecting pipe 509 integrally rotate, the orientation of the lifting member 507 and the connecting pipe 509 is changed, and the output end of the fifth stepping motor 506 drives the fifth screw rod 504 to rotate, so that the lifting member 507 moves up and down, and the connecting pipe 509 moves up and down.
Examples
Referring to fig. 15, the detecting mechanism 6 includes a placing plate 601, the placing plate 601 is fixedly mounted at the bottom end of the inner side of the right supporting plate 101, a plurality of groups of detecting cards 602 are equidistantly placed at the top of the placing plate 601, two groups of mounting blocks 603 are welded at the middle of the inner side of the right supporting plate 101, a sixth screw rod 604 is rotatably connected between the two groups of mounting blocks 603, a movable plate 607 is screwed on the outer surface of the sixth screw rod 604, a camera 608 is mounted at the bottom of the movable plate 607, a sixth guide rod 605 is mounted between the two groups of mounting blocks 603, the movable plate 607 is slidably connected to the outer surface of the sixth guide rod 605, a sixth stepping motor 606 is arranged at the outer side of one group of mounting blocks 603, and one end of the sixth screw rod 604 is fixedly connected to the output end of the sixth stepping motor 606.
As will be appreciated by those skilled in the art, the output end of the sixth stepper motor 606 drives the sixth screw rod 604 to rotate, so that the movable plate 607 slides reciprocally along the outer surface of the sixth guide rod 605, so that the camera 608 can move above each detection card 602, and the color development situation is photographed for the measurement personnel to understand.
Firstly, a plurality of groups of samples to be detected are placed in a sample storage tank 103 by a worker, two groups of first clamping pieces 219 and two groups of second clamping pieces 229 move to the position of a centrifuge tube 106 through the cooperation of a first stepping motor 203 and a first air cylinder 205, secondly, the two groups of first clamping pieces 219 mutually approach each other to clamp the centrifuge tube 106, then the output end of a third stepping motor 222 drives a third screw 220 to rotate, the heights of the two groups of second clamping pieces 229 are matched with the heights of plugs 107 at the top of the centrifuge tube 106, the two groups of second clamping pieces 229 mutually approach each other to clamp the plugs 107, then the output end of the third stepping motor 222 reversely rotates, the two groups of second clamping pieces 229 upwards move to pull out the plugs 107, the output end of an electric telescopic rod 224 is retracted to expose the top of the centrifuge tube 106, and the cooperation of a fourth stepping motor 304 and an electric push rod 306 is used to quantitatively add a mildly zymotic acid reagent A and a mildly zymotic acid reagent B into the centrifuge tube 106;
The output end of the electric telescopic rod 224 stretches and resets, the output end of the third stepping motor 222 drives two groups of second clamping pieces 229 and the clamped plugs 107 to move downwards, the plugs 107 are covered on the top of the centrifuge tube 106, the clamped centrifuge tube 106 is driven to move to the position of the sample storage tank 103 through the cooperation of the first stepping motor 203 and the first air cylinder 205, the top of the centrifuge tube 106 is exposed through the cooperation of the third stepping motor 222 and the electric telescopic rod 224, a first metering valve 104 is opened, samples are quantitatively added into the centrifuge tube 106, the rear plugs 107 are covered on the top of the centrifuge tube 106, the centrifuge tube 106 is driven to move up and down through the output end of the first air cylinder 205, and the shaking operation of a person is simulated, so that the samples are in a uniform dispersion state;
Then, through the matched use of the first stepping motor 203 and the first cylinder 205, the centrifuge tube 106 is placed in the placement hole 109, a cover on the centrifuge 108 is automatically closed, the working parameter of the centrifuge 108 is 4000r/min, the centrifugation time is 3min, in the centrifugation process, one centrifuge tube 106 is clamped again through the transferring mechanism 2 and is transferred to the inside of the fixing seat 404, the output ends of the two groups of second cylinders 405 extend and stretch, the two groups of first clamping plates 406 clamp the centrifuge tube 106, under the action of the third servo motor 411, the two groups of second clamping plates 412 clamp the plug 107 on the top of the centrifuge tube 106, the output end of the third cylinder 407 moves upwards to pull out the plug 107, after the centrifugation time is over, the plug 107 on the top of the centrifuge tube 108 is taken out through the transferring mechanism 2 and is placed in the waste collection box 110, the centrifuge tube 106 is still left in the centrifuge 108, the pipette gun 402 is clamped through the two groups of first clamping members 219, the two groups of second clamping members 229 clamp the top of the pipette 402, the two groups of second clamping members 403 clamp the centrifuge tube 106, the first clamping motors 205 clamp the centrifuge tube 106, the centrifuge tube 403 is moved up to the position of the second cylinder 403, and the centrifuge tube 106 is further moved up through the first clamping members 203, the piston heads 203 move upwards, the centrifuge tube 106 is moved up, and the supernatant is moved up to the position of the centrifuge tube 106, and the supernatant is moved up by the piston head 106, and the piston heads of the centrifuge tube 106 is moved up by the piston heads are moved up by the piston heads of the second clamping members, which are moved up to the piston heads, and the piston heads of the centrifuge heads are moved up;
transferring the centrifuge tube 106 filled with the supernatant into the sample concentrator 501, still clamping the plug 107 of the centrifuge tube 106 in two groups of second clamping plates 412, using the transmission motor 503 and the fifth stepping motor 506 in a matched manner to enable the connecting tube 509 to be inserted into the centrifuge tube 106, starting the heating device 508, controlling the temperature to be 60-65 ℃, sucking the liquid, transferring the centrifuge tube 106 to the bottom of the storage cylinder 112, adding the complex solution of the milbehenic acid into the centrifuge tube 106, transferring the centrifuge tube 106 to the position of the fixed seat 404, covering the plug 107, transferring the plug 107 to a flat gasket at the top of the vortex oscillator 114, performing vortex oscillation for 1min, transferring the liquid into the water cooling box 115, recovering the temperature to room temperature, preventing the subsequent scalding detection card 602, transferring the centrifuge tube 106 into the fixed seat 404, the plug 107 is removed, the transfer mechanism 2 clamps a new pipette 402 again and absorbs the new pipette, then the absorbed object to be detected is added into a sample adding hole of the detection card 602, the color development condition is observed after 8min, the operation can be performed on samples in other sample storage tanks 103, the detection continuity is ensured, manual operation by workers is not needed in the whole process, the detection of each sample does not share the centrifuge tube 106 and the pipette 402, the accuracy of detection data is ensured, the detection cost is lower in a reagent detection mode, the requirements of workers are further met, the color development condition of the detection card 602 is shown in fig. 16 finally, if a T line is deeper than a C line or the color development condition is as deep as possible, the result is negative, and the samples do not contain the milbemycetin or the concentration of the milbemycetous acid is lower than the detection limit; if the T line is shallower than the C line or the T line is not developed, the result is positive, which indicates that the concentration of the milbemycetin in the sample is higher than the detection limit, and the shallower the color development of the T line is than the C line, the higher the concentration of the milbemycetin in the sample is, and the centrifuge tube 106, the plug 107 and the pipette gun 402 used in the detection process are all collected in the waste collection box 110.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made therein without departing from the spirit and scope of the invention, which is defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103946712A (en) * | 2011-09-30 | 2014-07-23 | 不列颠哥伦比亚大学 | Methods and apparatus for flow-controlled wetting |
| CN117983120A (en) * | 2024-01-08 | 2024-05-07 | 武汉美丽有约生物科技有限公司 | Cosmetic emulsion raw material proportioning and blending device |
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| JP6180753B2 (en) * | 2013-02-15 | 2017-08-16 | 株式会社江東微生物研究所 | Microbiological testing system for food |
| CN112198324B (en) * | 2020-12-07 | 2021-06-15 | 北京慧荣和科技有限公司 | Full-automatic QuECHERS experimental device and experimental method |
| CN118683812A (en) * | 2024-07-29 | 2024-09-24 | 上海韩科科技有限公司 | Liquid chemical injection device with anti-leakage function |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103946712A (en) * | 2011-09-30 | 2014-07-23 | 不列颠哥伦比亚大学 | Methods and apparatus for flow-controlled wetting |
| CN117983120A (en) * | 2024-01-08 | 2024-05-07 | 武汉美丽有约生物科技有限公司 | Cosmetic emulsion raw material proportioning and blending device |
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