CN118858270B - Test paper for detecting concentration of phthalic dicarboxaldehyde disinfectant - Google Patents
Test paper for detecting concentration of phthalic dicarboxaldehyde disinfectant Download PDFInfo
- Publication number
- CN118858270B CN118858270B CN202411283006.4A CN202411283006A CN118858270B CN 118858270 B CN118858270 B CN 118858270B CN 202411283006 A CN202411283006 A CN 202411283006A CN 118858270 B CN118858270 B CN 118858270B
- Authority
- CN
- China
- Prior art keywords
- layer
- test paper
- concentration
- detection
- self
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 77
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 title claims abstract description 73
- 239000000645 desinfectant Substances 0.000 title claims abstract description 24
- 238000001514 detection method Methods 0.000 claims abstract description 98
- 238000006243 chemical reaction Methods 0.000 claims abstract description 47
- 239000004005 microsphere Substances 0.000 claims abstract description 38
- 239000000017 hydrogel Substances 0.000 claims abstract description 33
- 230000004044 response Effects 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000003623 enhancer Substances 0.000 claims abstract description 17
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 17
- 229940054441 o-phthalaldehyde Drugs 0.000 claims abstract description 12
- 238000005516 engineering process Methods 0.000 claims abstract description 10
- 238000010521 absorption reaction Methods 0.000 claims abstract description 9
- 239000010410 layer Substances 0.000 claims description 83
- 238000000034 method Methods 0.000 claims description 27
- 239000002245 particle Substances 0.000 claims description 21
- -1 polyethylene terephthalate Polymers 0.000 claims description 20
- 239000012528 membrane Substances 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 17
- 239000011241 protective layer Substances 0.000 claims description 16
- 239000004698 Polyethylene Substances 0.000 claims description 15
- 239000010931 gold Substances 0.000 claims description 15
- 229910052737 gold Inorganic materials 0.000 claims description 15
- 229920000573 polyethylene Polymers 0.000 claims description 15
- 230000008859 change Effects 0.000 claims description 14
- 229940125532 enzyme inhibitor Drugs 0.000 claims description 14
- 239000002532 enzyme inhibitor Substances 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 13
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 claims description 12
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 claims description 12
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 12
- QNILTEGFHQSKFF-UHFFFAOYSA-N n-propan-2-ylprop-2-enamide Chemical compound CC(C)NC(=O)C=C QNILTEGFHQSKFF-UHFFFAOYSA-N 0.000 claims description 12
- 238000007639 printing Methods 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 239000002033 PVDF binder Substances 0.000 claims description 11
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 11
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 11
- 239000012086 standard solution Substances 0.000 claims description 11
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical group NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 claims description 10
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 10
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical class C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 9
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 9
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 239000004743 Polypropylene Substances 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 8
- 229920001155 polypropylene Polymers 0.000 claims description 8
- 239000002096 quantum dot Substances 0.000 claims description 8
- DMLAVOWQYNRWNQ-UHFFFAOYSA-N azobenzene Chemical class C1=CC=CC=C1N=NC1=CC=CC=C1 DMLAVOWQYNRWNQ-UHFFFAOYSA-N 0.000 claims description 7
- 239000011248 coating agent Substances 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 6
- 239000005515 coenzyme Substances 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 6
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 6
- 229920002301 cellulose acetate Polymers 0.000 claims description 5
- 239000003431 cross linking reagent Substances 0.000 claims description 5
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 claims description 5
- 238000006116 polymerization reaction Methods 0.000 claims description 5
- 239000004094 surface-active agent Substances 0.000 claims description 5
- FBWADIKARMIWNM-UHFFFAOYSA-N N-3,5-dichloro-4-hydroxyphenyl-1,4-benzoquinone imine Chemical compound C1=C(Cl)C(O)=C(Cl)C=C1N=C1C=CC(=O)C=C1 FBWADIKARMIWNM-UHFFFAOYSA-N 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
- 239000003999 initiator Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000178 monomer Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 239000000020 Nitrocellulose Substances 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 229920001220 nitrocellulos Polymers 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- MUUHXGOJWVMBDY-UHFFFAOYSA-L tetrazolium blue Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC=CC=2)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 MUUHXGOJWVMBDY-UHFFFAOYSA-L 0.000 claims description 3
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 2
- BOPGDPNILDQYTO-NNYOXOHSSA-L NADH(2-) Chemical group C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-L 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 150000001988 diarylethenes Chemical class 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims description 2
- 230000002538 fungal effect Effects 0.000 claims description 2
- 239000003365 glass fiber Substances 0.000 claims description 2
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 2
- AYGYHGXUJBFUJU-UHFFFAOYSA-N n-[2-(prop-2-enoylamino)ethyl]prop-2-enamide Chemical compound C=CC(=O)NCCNC(=O)C=C AYGYHGXUJBFUJU-UHFFFAOYSA-N 0.000 claims description 2
- 229920000728 polyester Polymers 0.000 claims description 2
- 239000004065 semiconductor Chemical class 0.000 claims description 2
- 125000003396 thiol group Chemical group [H]S* 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 11
- 230000007613 environmental effect Effects 0.000 abstract description 9
- 238000012544 monitoring process Methods 0.000 abstract description 4
- 230000000007 visual effect Effects 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 10
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical group [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 8
- 238000013461 design Methods 0.000 description 8
- 230000006872 improvement Effects 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 238000004445 quantitative analysis Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 238000013523 data management Methods 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 229910021389 graphene Inorganic materials 0.000 description 5
- 230000002829 reductive effect Effects 0.000 description 5
- 239000001993 wax Substances 0.000 description 5
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 239000012188 paraffin wax Substances 0.000 description 4
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 125000004203 4-hydroxyphenyl group Chemical group [H]OC1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 3
- 239000005711 Benzoic acid Substances 0.000 description 3
- 229920002799 BoPET Polymers 0.000 description 3
- 235000010233 benzoic acid Nutrition 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000007405 data analysis Methods 0.000 description 3
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000012764 semi-quantitative analysis Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- DKIDEFUBRARXTE-UHFFFAOYSA-N 3-mercaptopropanoic acid Chemical compound OC(=O)CCS DKIDEFUBRARXTE-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical group [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 244000172533 Viola sororia Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000005562 fading Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 2
- 229950006238 nadide Drugs 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 2
- 238000007650 screen-printing Methods 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- VCESGVLABVSDRO-UHFFFAOYSA-L 2-[4-[4-[3,5-bis(4-nitrophenyl)tetrazol-2-ium-2-yl]-3-methoxyphenyl]-2-methoxyphenyl]-3,5-bis(4-nitrophenyl)tetrazol-2-ium;dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC(=CC=2)[N+]([O-])=O)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC(=CC=2)[N+]([O-])=O)=NN1C1=CC=C([N+]([O-])=O)C=C1 VCESGVLABVSDRO-UHFFFAOYSA-L 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 1
- 235000005811 Viola adunca Nutrition 0.000 description 1
- 240000009038 Viola odorata Species 0.000 description 1
- 235000013487 Viola odorata Nutrition 0.000 description 1
- 235000002254 Viola papilionacea Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000007697 cis-trans-isomerization reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006011 modification reaction Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 125000000962 organic group Chemical group 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000007699 photoisomerization reaction Methods 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000012744 reinforcing agent Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
- 239000004289 sodium hydrogen sulphite Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- KUCOHFSKRZZVRO-UHFFFAOYSA-N terephthalaldehyde Chemical compound O=CC1=CC=C(C=O)C=C1 KUCOHFSKRZZVRO-UHFFFAOYSA-N 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Plasma & Fusion (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
本发明涉及检测技术领域的一种邻苯二甲醛消毒液浓度检测试纸，包括疏水材料制成的底层；设置于底层上的微流控层，由疏水边界划分出多个功能区域；设置于微流控层上的智能响应检测层，包含温度响应型水凝胶微球和信号增强剂；设置于微流控层上的自校准层；位于试纸一端的样品输入区，设有吸水层；以及设置于智能响应检测层、自校准层和集成比色卡上方的可变色指示元件，使用时，将样品添加至样品输入区，用紫外光激活，待反应完成后，可通过目视比对或智能设备扫描获取结果，本发明具有高灵敏度、特异性强、操作简便、检测快速、结果准确等优点，适用于医疗卫生、食品安全、环境监测等多个领域的邻苯二甲醛浓度快速检测。
The invention relates to a test paper for detecting concentration of o-phthalaldehyde disinfectant in the field of detection technology. The test paper comprises a bottom layer made of a hydrophobic material; a microfluidic layer arranged on the bottom layer, and a plurality of functional areas are divided by hydrophobic boundaries; an intelligent response detection layer arranged on the microfluidic layer, and comprising temperature-responsive hydrogel microspheres and a signal enhancer; a self-calibration layer arranged on the microfluidic layer; a sample input area at one end of the test paper, and provided with a water absorption layer; and a color-variable indicating element arranged above the intelligent response detection layer, the self-calibration layer and an integrated colorimetric card. When in use, a sample is added to the sample input area and activated with ultraviolet light. After the reaction is completed, a result can be obtained by visual comparison or scanning with an intelligent device. The invention has the advantages of high sensitivity, strong specificity, simple operation, rapid detection, accurate results, and the like, and is suitable for rapid detection of o-phthalaldehyde concentration in multiple fields such as medical care, food safety, and environmental monitoring.
Description
Technical Field
The invention relates to a test paper for detecting the concentration of a phthalaldehyde disinfectant, in particular to a test paper for detecting the concentration of a phthalaldehyde disinfectant, which is applied to the technical field of detection.
Background
The phthalic dicarboxaldehyde is a widely used high-efficiency disinfectant and is widely applied to the fields of medical instrument disinfection and the like. Accurate and rapid detection of its concentration is critical to ensure disinfection efficacy and safety. There are some methods in the prior art for detecting the concentration of phthalic aldehyde, but there are some limitations.
Chinese patent No. 107024473B discloses a rapid detection test paper for the concentration of phthalic dicarboxaldehyde disinfectant, and a preparation method and application thereof. The test paper is tested by immersing a solution of a specific component in a filter paper. The method is simple to operate, can rapidly detect the content of the phthalic dicarboxaldehyde, and is beneficial to medical staff to monitor the concentration of the disinfectant at any time. However, the detection sensitivity and specificity of this method still have room for improvement, and it is difficult to achieve accurate quantitative analysis.
Chinese patent No. CN106970075B discloses another test paper for detecting the concentration of phthalic aldehyde and a preparation method thereof. The test paper judges whether the concentration reaches a specified threshold value or not through color change by utilizing the reaction principle of sodium bisulphite and phthalic aldehyde. The method is simple and convenient to use, and the result is accurate and reliable. But it is mainly limited to the judgment of a specific threshold (0.35%), and it is difficult to meet the detection requirements of different concentration ranges.
The rapid detection of the concentration of the phthalic aldehyde is realized by the chemical reaction principle, but the detection method has certain limitations, such as insufficient detection sensitivity, limited specificity, difficulty in realizing accurate quantification, narrow detection range and the like. In addition, the prior art lacks intelligent and data management functions, and is difficult to meet the requirements of the modern medical and industrial fields on data tracking and analysis.
In practical applications, different detection sensitivity and range may be required for different scenarios. For example, during sterilization of medical instruments, an accurate determination of whether the phthalaldehyde concentration has reached an effective sterilization level is required, while in environmental monitoring, it may be desirable to detect a lower concentration of phthalaldehyde residues. Therefore, it is of great practical significance to develop a detection method which has high sensitivity, wide detection range, quantitative analysis and simple operation.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to solve the technical problem of developing the o-phthalaldehyde disinfectant concentration detection test paper which has high sensitivity, high specificity, wide detection range, quantitative analysis and simple operation.
In order to solve the problems, the invention provides a phthalic aldehyde disinfectant concentration detection test paper, which comprises a bottom layer made of a hydrophobic material;
The microfluidic layer is arranged on the bottom layer, and a plurality of functional areas are divided by the hydrophobic boundary;
The intelligent response detection layer is arranged on the microfluidic layer and comprises temperature response hydrogel microspheres and a signal enhancer;
the self-alignment layer is arranged on the microfluidic layer;
the sample input area is positioned at one end of the test paper and is provided with a water absorption layer;
the result display area is positioned at the other end of the test paper and is provided with an integrated color chart;
And the variable color indicating element is arranged above the intelligent response detection layer, the self-calibration layer and the integrated color chart.
In the phthalic aldehyde disinfectant concentration detection test paper, the detection sensitivity and specificity are improved, the detection range is enlarged, the requirements of different application scenes are met, the self-calibration function is integrated, the reliability and accuracy of the detection result are improved, the visual and intelligent detection method is combined, the visual and intelligent detection method can be used for fast judgment by naked eyes, accurate and quantitative analysis can be realized by intelligent equipment, the data management function is provided, and the detection result can be recorded, stored and analyzed conveniently.
As a further improvement of the present application, the plurality of functional areas includes a sample input area, a reaction area, a self-calibration area, and a result display area.
As a still further improvement of the present application, the bottom layer is made of at least one material of polyethylene terephthalate (PET), polypropylene (PP) or Polyethylene (PE);
the microfluidic layer is made of at least one hydrophilic porous membrane material selected from polyvinylidene fluoride (PVDF) membrane, nitrocellulose membrane or cellulose acetate membrane;
The hydrophobic boundary is formed of at least one material of wax, polydimethylsiloxane (PDMS), or a photo-curable resin.
As a further improvement of the application, the temperature-responsive hydrogel microsphere is prepared by copolymerizing N-isopropyl acrylamide (NIPAM) and at least one crosslinking agent of N, N '-methylene bisacrylamide or N, N' -ethylene bisacrylamide.
As a further improvement of the application, the temperature-responsive hydrogel microsphere is encapsulated with functionalized nano gold particles with the particle size of 5-100nm, the surfaces of the functionalized nano gold particles are modified with sulfhydryl compounds, an enzyme system comprising aldehyde dehydrogenase, coenzyme and an electron acceptor, and a photosensitive enzyme inhibitor.
As a complement to a further improvement of the application, the aldehyde dehydrogenase is selected from aldehyde dehydrogenases of bacterial or fungal origin;
The coenzyme is nicotinamide adenine dinucleotide (NAD+) or reduced form (NADH) thereof;
the electron acceptor is selected from at least one of tetrazolium blue (NBT), dichloroindophenol (DCIP) or methylene blue;
The photosensitive enzyme inhibitor is selected from at least one of azobenzene derivative, spiropyran derivative or diarylethene derivative.
As a further improvement of the present application, the signal enhancer is selected from at least one of graphene quantum dots, carbon quantum dots, or semiconductor quantum dots;
when the signal enhancer is graphene quantum dots, the particle size of the signal enhancer is 1-20nm, and the emission wavelength is 400-700nm;
The self-calibration layer comprises a preset phthalic aldehyde solution with known concentration, and the concentration range of the phthalic aldehyde solution is 0.1% -1.0%;
the water absorption layer is made of at least one material selected from modified cellulose, glass fiber or polyester fiber.
As a further improvement of the present application, the integrated color chart comprises a plurality of color areas, each color area corresponding to a predetermined phthalic aldehyde concentration range;
The color-changeable indicating element is a two-dimensional code or a bar code printed by using ink which can be changed along with the concentration of the phthalic aldehyde;
The test paper also comprises a strippable transparent protective layer which is covered on the surface of the test paper, wherein the protective layer is made of at least one material selected from polyethylene, polypropylene or polyethylene terephthalate.
A preparation method of a phthalic dicarboxaldehyde disinfectant concentration detection test paper comprises the following steps of;
s1, creating a microfluidic channel on a hydrophilic porous membrane by using a hydrophobic material printing technology;
S2, preparing temperature-responsive hydrogel microspheres containing functionalized nano-gold particles, an enzyme system and a photosensitive enzyme inhibitor;
S3, coating the hydrogel microsphere suspension liquid on a reaction area;
s4, applying a signal enhancer on the hydrogel microsphere layer;
s5, adding a phthalic aldehyde standard solution with known concentration in a self-calibration area;
S6, setting an integrated color chart and a variable color indicating element;
S7, assembling a bottom layer, the treated hydrophilic porous membrane and a water absorption layer;
S8, covering a protective layer;
s9, treating the coated test paper by using a freeze-drying technology, wherein the freeze-drying is carried out at a temperature of between 40 ℃ below zero and 60 ℃ below zero and at a pressure of between 0.01 and 0.1mbar for 6 to 24 hours;
The method for preparing the hydrogel microsphere in the step S2 comprises the following steps:
M1, dispersing N-isopropyl acrylamide monomer, a cross-linking agent, functionalized nano gold particles, an enzyme system and a photosensitive enzyme inhibitor in an oil phase in the presence of a water-oil surfactant;
M2, under the protection of nitrogen, adding an initiator to perform polymerization reaction;
and M3, centrifugally separating to obtain hydrogel microspheres, and washing with deionized water.
The application method of the phthalic dicarboxaldehyde disinfectant concentration detection test paper comprises the following steps of;
b1, adding a phthalic aldehyde sample to be detected into a sample input area;
B2, irradiating the test paper for 5-15 seconds by using light with the wavelength of 350-400nm so as to activate detection reaction;
b3, waiting for 30 seconds to 5 minutes at 15-30 ℃ to complete the reaction;
B4, observing the color changes of the reaction area and the self-calibration area, and comparing the color with the integrated color chart;
b5, using intelligent equipment to scan the variable color indicating element to obtain quantitative result
And B6, analyzing the color change of the variable color indicating element by using a special application program on the intelligent equipment, calculating the accurate concentration of the phthalic aldehyde, and recording, storing and transmitting the test result.
In summary, the application has the following beneficial effects:
1. The temperature response type hydrogel microsphere is combined with a specific enzyme system, so that the high-selectivity detection of the p-phthalaldehyde is realized, the theoretical detection limit is high, the strict detection requirement is met, the linear detection range is wide, and the common concentration range in practical application is covered.
2. The method has the advantages of high speed, simple operation, short detection process time, high detection efficiency, simple operation steps, no need of complex sample pretreatment and simple illumination, suitability for non-professional personnel, and contribution to popularization and application.
3. The integrated color chart supports quick visual semi-quantitative analysis, meets the requirement of on-site quick detection, realizes accurate quantitative analysis by matching the color-changeable two-dimensional code with intelligent equipment, provides professional-level detection results, flexibly meets the requirements of different use scenes and precision, and enhances the applicability of products.
4. The self-calibration and anti-interference, the built-in self-calibration area remarkably improves the reliability of detection results, the design of the temperature response hydrogel microsphere and the functional nano gold particles enhances the anti-interference capability, effectively reduces the influence of environmental factors (such as temperature and humidity) and improves the detection accuracy.
5. The freeze drying treatment and the strippable protective layer improve the stability and the shelf life of the test paper, are small and light, are easy to carry and store, are suitable for on-site rapid detection, and expand the application scene.
6. The detection result can be conveniently recorded, stored and transmitted through intelligent equipment scanning, and the special application program supports long-term data management and analysis, so that the detection database can be built, the technical platform has good expansibility, and the detection system can be used for detecting other types of aldehyde substances through replacing a specific enzyme system.
Drawings
FIG. 1 is an overall exploded view of the present application;
FIG. 2 is a top view of the present application;
FIG. 3 is a cross-sectional view A-A of FIG. 2 in accordance with the present application;
FIG. 4 is a cross-sectional view B-B of FIG. 3 in accordance with the present application;
fig. 5 is an external view of the present application.
The reference numerals in the figures illustrate:
1. a bottom layer; 2, a micro-fluidic layer, 3, an intelligent response detection layer, 4, a self-calibration layer, 5, a water absorption layer and 6, an integrated colorimetric card.
Detailed Description
Three embodiments of the present application will be described in detail with reference to the accompanying drawings.
Example 1:
Fig. 1-5 show that the embodiment provides a test paper for detecting the concentration of the phthalic aldehyde disinfectant, and the structure of the test paper sequentially comprises a bottom layer 1, a microfluidic layer 2, an intelligent response detection layer 3, a self-calibration layer 4 and a variable color indicating element from bottom to top. One end of the test paper is provided with a sample input area, and the other end is provided with a result display area. The following is detailed below:
The bottom layer 1 is made of a hydrophobic material and mainly plays roles in supporting and waterproof. In this embodiment, the bottom layer 1 is made of polyethylene terephthalate (PET) material, and has a thickness of 0.1mm. PET has excellent mechanical strength and chemical stability, and can provide good support for the whole test paper system. In addition, polypropylene (PP) or Polyethylene (PE) can be selected as alternative materials, which have good hydrophobicity and chemical resistance.
The microfluidic layer 2 is arranged on the bottom layer 1 and is made of a hydrophilic porous membrane material. In this example, a polyvinylidene fluoride (PVDF) membrane was used, having a pore size of 0.45 μm and a thickness of 100. Mu.m. The PVDF film has good chemical stability and mechanical strength, and is suitable for use as a base material for the microfluidic layer 2. In addition, nitrocellulose or cellulose acetate membranes may be used as alternatives.
The microfluidic layer 2 is divided into a plurality of functional areas by hydrophobic boundaries, including a sample input area, a reaction area, a self-calibration area and a result display area. The hydrophobic boundary was made using a molten wax printing technique using paraffin wax as the starting material. The wax has a melting point of about 60 ℃ and is sprayed to a predetermined location by a precision ink jet printer during printing and cooled to form a hydrophobic boundary. The width of these boundaries is 0.5mm and the height is about 1.5 times the PVDF film thickness, i.e., 150 μm. In addition to wax, polydimethylsiloxane (PDMS) or a photocurable resin may be selected as the hydrophobic boundary material.
The intelligent response detection layer 3 is arranged above the reaction area of the microfluidic layer 2 and mainly comprises temperature response hydrogel microspheres and a signal enhancer. The detailed composition and preparation method of the hydrogel microspheres will be described in detail in the following examples. The signal enhancer is graphene quantum dot, the particle size is 5nm, and the emission wavelength is 520nm.
The self-calibration layer 4 is arranged above the self-calibration area of the microfluidic layer 2 and contains a preset phthalic aldehyde solution with known concentration. In this example, a standard solution of 0.5% strength phthalic aldehyde was used, and the volume was 2. Mu.L.
The sample input area is located the one end of test paper, is equipped with the layer 5 that absorbs water. The water absorption layer 5 is made of modified cellulose material, has the thickness of 0.5mm, and can effectively absorb and uniformly distribute a sample to be detected.
The result display area is positioned at the other end of the test paper and is provided with an integrated color chart 6. The color card contains 6 color zones corresponding to 0%, 0.2%, 0.4%, 0.6%, 0.8% and 1.0% o-phthalaldehyde concentrations, respectively. The size of each color area was 3mm by 3mm, and was printed with a fade-resistant ink.
The variable color indicating element is arranged above the intelligent response detection layer 3, the self calibration layer 4 and the integrated color chart 6. In the embodiment, the two-dimensional code is printed by using the photochromic ink which can be changed along with the concentration of the phthalic aldehyde. Located beside the results display area.
In order to prevent the test paper from being wetted or polluted, a strippable transparent protective layer is covered on the whole surface of the test paper. The protective layer is made of Polyethylene (PE) film with the thickness of 0.05mm, and can be easily torn off when in use.
According to the phthalic dicarboxaldehyde disinfectant concentration detection test paper, through reasonable design and material selection, automatic flow, accurate reaction and convenient reading of samples are realized. The design of the microfluidic layer 2 ensures orderly flow of samples among the functional areas, the intelligent response detection layer 3 provides a high-sensitivity detection mechanism, and the self-calibration layer 4 and the integrated color chart 6 further improve detection accuracy. The introduction of the color-changeable indicating element enables the detection result to be rapidly read and analyzed through the intelligent equipment, and the use convenience is greatly improved.
The following is a detailed procedure of the test paper for detecting the concentration of the o-phthalaldehyde sterilizing liquid described in example 1, in which a user first tears the PE protective layer on the surface of the test paper and then drops about 50. Mu.L of the o-phthalaldehyde sterilizing liquid sample to be detected into the sample input area of the test paper.
Sample flow, sample is rapidly absorbed and begins to flow to the right along the microfluidic layer 2 due to capillary action of the water-absorbing layer 5. The hydrophobic boundary effectively controls the flow direction and velocity of the sample, preventing the sample from diffusing into unintended areas.
And (3) the reaction process, namely, when the sample flows to the reaction zone, the temperature-responsive hydrogel microspheres in the intelligent response detection layer3 start to change. The aldehyde dehydrogenase encapsulated in the microsphere reacts specifically with the phthalaldehyde while the coenzyme NAD+ is reduced to NADH. NADH then reacts with an electron acceptor (e.g., NBT) to produce a visible bluish violet precipitate.
The optical signal generated in the reaction process is enhanced by the graphene quantum dots, so that the detection sensitivity is improved. The strong fluorescence emission of graphene quantum dots at 520nm wavelength further enhances the visibility of color changes.
Self-calibration, wherein the sample simultaneously flows through the self-calibration area and reacts with the preset 0.5% o-phthalaldehyde standard solution in the same way. This process provides an internal reference for each test that helps to eliminate the effects of environmental factors (e.g., temperature, humidity).
The self-calibration process can correct the effect of environmental factors (e.g., temperature, humidity) on the test results by comparison with standard solutions of known concentrations. Specifically, by comparing the color change of the self-calibration region with the color change of the reaction region, a correction factor can be calculated. By applying this correction factor to the reaction zone results, a more accurate value of the phthalaldehyde concentration can be obtained.
The results showed that after about 2-3 minutes, the sample reached the results display area. The user can directly compare the color change of the reaction area and the self-calibration area with the integrated color chart 6, and the concentration of the phthalic aldehyde is estimated by naked eyes.
And in order to obtain a more accurate quantitative result, a user can scan the variable color indicating element (two-dimensional code) by using the smart phone. The color change of the two-dimensional code is in direct proportion to the concentration of the phthalic aldehyde, and the concentration of the phthalic aldehyde in the sample can be accurately calculated through a special mobile phone application program.
The design of the enzymatic reaction and temperature responsive hydrogel microspheres in the smart responsive detection layer 3 ensures high sensitivity and specificity of detection. The theoretical detection limit can reach 0.01% of the concentration of the phthalic dicarboxaldehyde.
The whole detection process only needs 2-3 minutes, so that the time required by the traditional detection method is shortened.
When in use, only the sample is needed to be added, other complex operations are not needed, and the use threshold is reduced.
Through naked eye comparison and intelligent equipment scanning, quick qualitative judgment and accurate quantitative analysis are realized simultaneously.
The built-in self-calibration area improves the reliability of the detection result and reduces the interference of environmental factors.
The color-changeable indicating element (two-dimensional code) is not only used for reading results, but also can be used as an anti-counterfeiting mark to prevent counterfeit products.
Through intelligent device scanning, detection results can be recorded, stored and transmitted conveniently, and long-term data management and analysis are facilitated.
The test paper is not only suitable for the field of medical and health, but also can be applied to the rapid detection of the concentration of the phthalic aldehyde in a plurality of fields such as food safety, environmental monitoring and the like.
Example 2:
Fig. 1 to 5 show that this embodiment mainly describes the detailed composition of the intelligent response detection layer 3 in the test paper for detecting the concentration of phthalic aldehyde disinfectant and the preparation method thereof.
The temperature response type hydrogel microsphere comprises the following raw materials of N-isopropyl acrylamide (NIPAM), the mass fraction of the main monomer is 95%, the mass fraction of the cross-linking agent is 5%, the initiator is Ammonium Persulfate (APS), the concentration of the initiator is 0.1mol/L, the surfactant is Sodium Dodecyl Sulfate (SDS), and the concentration of the surfactant is 0.2mmol/L.
The preparation method comprises the steps of dissolving 0.95gNIPAM g and 0.05g of N, N' -methylene bisacrylamide in 50mL of deionized water, adding 10: 10mgSDS, stirring uniformly, introducing nitrogen into the solution for 20 minutes to remove oxygen, heating the reaction mixture in a 70 ℃ water bath, rapidly adding 1mL of 0.1mol/LAPS solution to start polymerization, reacting for 4 hours at 70 ℃, cooling to room temperature, dialyzing for 48 hours to remove unreacted monomers and impurities, and freeze-drying to obtain the temperature-responsive hydrogel microsphere.
Preparing functionalized nano gold particles, raw materials, chloroauric acid (HAuCl 4) with the concentration of 1mmol/L, sodium citrate with the concentration of 38.8mmol/L and 3-mercaptopropionic acid with the concentration of 1mmol/L;
The preparation method comprises the steps of heating 50mLHAuCl mL of sodium citrate solution to boil, continuously stirring, continuously boiling for 15 minutes, changing the solution from pale yellow to reddish wine, cooling to room temperature, adding 1mL of 3-mercaptopropionic acid solution, stirring at room temperature for 2 hours, centrifugally purifying, and re-suspending in deionized water, wherein the average particle size of the finally obtained functionalized nano gold particles is 15nm.
The composition of an enzyme system, the activity of aldehyde dehydrogenase is more than or equal to 5U/mg protein, the purity of coenzyme beta-nicotinamide adenine dinucleotide (NAD+), the purity of the coenzyme beta-nicotinamide adenine dinucleotide is more than or equal to 98%, and the purity of an electron acceptor tetrazolium blue (NBT) is more than or equal to 98%;
the photosensitive enzyme inhibitor is azobenzene derivative 4- ((4-hydroxyphenyl) azo) benzoic acid with purity more than or equal to 97 percent.
Preparation of the Intelligent response detection layer 3, dispersing 1mg of temperature response hydrogel microspheres in 1mLPBS buffer (pH 7.4), adding 100 μl of functionalized nano-gold particle solution, incubating at room temperature for 2 hours, adding aldehyde dehydrogenase (final concentration 0.5U/mL), NAD+ (final concentration 1 mmol/L) and NBT (final concentration 0.5 mmol/L), finally adding photosensitive enzyme inhibitor (final concentration 0.1 mmol/L), lightly coating the mixture on a reaction area of test paper, and drying at room temperature.
The signal enhancer is prepared by uniformly spraying graphene quantum dot solution with the concentration of 1mg/mL above the intelligent response detection layer 3 in the dosage of 5 mu L/cm < 2 >, wherein the graphene quantum dot is selected as the signal enhancer and has the characteristics of average particle diameter of 5nm, emission wavelength of 520nm and quantum yield of 15%.
Self-alignment layer 4, carrier, cellulose acetate film with pore diameter of 0.22 μm, and standard solution, 0.5% o-phthalaldehyde water solution.
The preparation method comprises the steps of dripping 2 mu L of 0.5% o-phthalaldehyde standard solution on a cellulose acetate membrane with the size of 1cm < 2 >, drying in vacuum at 37 ℃ for 1 hour, and fixing the dried membrane in a self-alignment area of test paper.
The number of the integrated color cards 6 is 6, and the corresponding concentration ranges are 0%, 0.2%, 0.4%, 0.6%, 0.8% and 1.0%.
The manufacturing method comprises the steps of designing standard color blocks corresponding to each concentration by using color management software, printing the color blocks on a PET film by using anti-fading ink and a high-precision screen printing technology, and cutting and fixing the printed color chart in a result display area of test paper.
The method for manufacturing the color-changeable indicating element comprises the steps of designing a two-dimensional code containing product information and a serial number, preparing photosensitive color-changeable ink, mixing photosensitive dye (such as spiropyran) with polymer matrix (such as polymethyl methacrylate), spraying the photosensitive color-changeable ink on test paper according to a two-dimensional code pattern by using a micro-spray printing technology, and curing for 10 seconds under 365nm ultraviolet light.
The manufacturing method of the protective layer comprises the steps of selecting a food-grade PE film, cutting the film into the same size as the test paper, coating low-viscosity peelable glue on one side of the film, covering the glue-coated surface downwards on the surface of the test paper, lightly pressing to ensure the lamination, and designing an easily-torn pull piece on the exposed part of the protective layer, so that the protective layer is convenient to peel during use.
The method comprises the steps of integrally assembling, assembling a bottom layer 1, a microfluidic layer 2 and an intelligent response detection layer 3 according to the basic structure described in the embodiment 1, fixing a self-calibration layer 4 at a specified position, pasting an integrated color chart 6 on a result display area, printing a two-dimensional code by using photosensitive color-changing ink, and finally covering a protective layer.
After the o-phthalaldehyde disinfectant sample is added to the input end of the test paper, the sample flows along the microfluidic channel to the detection area due to capillary action.
And after the sample reaches the intelligent response detection layer 3, the temperature response hydrogel microsphere starts to change. At room temperature (about 25 ℃), the microspheres are in an expanded state, allowing the phthalaldehyde molecules in the sample to enter the interior of the microspheres.
The aldehyde dehydrogenase in the microsphere reacts with the phthalic aldehyde in a specific way. In the presence of NAD+, phthalic aldehyde is oxidized to phthalic acid while NAD+ is reduced to NADH.
The generated NADH reacts with the electron acceptor NBT, and NBT is reduced to form blue-purple formazan. This process produces a visible color change.
The graphene quantum dots are used as signal reinforcing agents, and the optical signals of reaction products are enhanced through Fluorescence Resonance Energy Transfer (FRET) effect, so that the detection sensitivity is improved.
Light-operated regulation, and photosensitive enzyme inhibitor (azobenzene derivative) plays a role in regulating in the detection process. In the detection, the user needs to irradiate the test paper with 365nm ultraviolet light for about 10 seconds, which causes the configuration of the inhibitor to change, releases the inhibition of the enzyme, and starts the detection reaction, and the photosensitive enzyme inhibitor (such as 4- ((4-hydroxyphenyl) azo) benzoic acid is in a trans configuration under dark conditions, so that the activity of aldehyde dehydrogenase can be inhibited. When exposed to 365nm ultraviolet light, cis-trans isomerization occurs, the cis-configuration is converted, and inhibition effect on enzyme is lost, so that detection reaction is started. The design can accurately control the starting time of the reaction, and improve the accuracy and reproducibility of detection.
And the sample simultaneously flows through the self-calibration area and reacts with the preset 0.5% o-phthalaldehyde standard solution in the same way. This procedure provides an internal reference standard for each test.
And after about 2-3 minutes, a user can read the result by directly comparing the color change of the reaction area and the self-calibration area with the integrated color comparison card 6, performing semi-quantitative analysis, scanning the variable color two-dimensional code by using a smart phone, and performing quantitative analysis by using a special application program.
The design of the temperature responsive hydrogel microsphere allows for detection with a high degree of specificity. The microsphere expands at room temperature, allows target molecules to enter, and contracts after the reaction is completed, so that interfering substances are effectively blocked. The theoretical detection limit can reach 0.01% of the concentration of the phthalic dicarboxaldehyde.
By optimizing the composition and the reaction condition of the enzyme system, the detection range can cover the concentration of the phthalic dicarboxaldehyde of 0.1-1.0%, thereby meeting most of practical application requirements.
The surface modification of the functionalized nano gold particles improves the stability of the system, reduces non-specific adsorption and enhances the anti-interference capability.
The graphene quantum dot is used as a signal enhancer, and the detection sensitivity is remarkably improved through the FRET effect, so that a low-concentration sample can be accurately detected.
The introduction of the photosensitive enzyme inhibitor realizes the accurate control of the detection process, avoids the early reaction of the test paper in the storage process, and prolongs the effective period of the test paper.
The built-in self-calibration area improves the reliability of the detection result, effectively reduces the interference of environmental factors (such as temperature and humidity) and improves the detection accuracy.
The integrated color chart 6 allows rapid semi-quantitative analysis, and the color-changeable two-dimensional code supports accurate quantitative analysis through intelligent equipment, so that the requirements of different use scenes are met.
Example 3:
Fig. 1 to 5 show that the preparation method and the use method of the test paper for detecting the concentration of the phthalic aldehyde disinfectant are described in detail in the embodiment.
S1, creating a microfluidic channel, designing a microfluidic channel pattern by using CAD software, inputting the design pattern into a wax printer, fixing a PVDF film on a printing platform, heating paraffin to 65 ℃ to melt the paraffin, printing molten paraffin on the PVDF film by using a precise ink-jet technology, and naturally cooling to room temperature to form a hydrophobic boundary.
S2, preparing temperature response hydrogel microspheres
Step M1. A reaction mixture was prepared, 50mL of cyclohexane, 10mL of deionized water, 50mg of N-isopropylacrylamide (NIPAM), 50mg of N, N' -methylenebisacrylamide, 1mL of functionalized nano gold particles (concentration 1 mg/mL), 10mg of aldehyde dehydrogenase, 20mg of NAD+ (NAD+), 10mg of NBT, 5mg of photosensitive enzyme inhibitor (4- ((4-hydroxyphenyl) azo) benzoic acid, 80 mg of Span, 1mL of surfactant, and the mixture was mixed and dispersed by ultrasonic waves for 5 minutes.
Step M2. Polymerization reaction the mixture in M1 was put into a three-necked flask containing an oil phase, purged with nitrogen for 15 minutes, stirred in a 70 ℃ water bath at 500rpm, and rapidly polymerized by adding 0.1mL of 0.1mol/L Ammonium Persulfate (APS) solution for 4 hours.
Step M3, after the reaction, the mixture is cooled to room temperature, centrifuged at 6000rpm for 10 minutes, the precipitate is collected, washed with deionized water and ethanol alternately for 3 times, and finally dispersed in 10mLPBS buffer (pH 7.4).
S3, coating hydrogel microspheres, uniformly coating 100 mu L of hydrogel microsphere suspension on a reaction area by using a microsampler, and naturally drying at room temperature for 2 hours.
S4, applying a signal enhancer, preparing graphene quantum dot solution with the concentration of 1mg/mL, uniformly spraying quantum dot solution with the concentration of 5 mu L/cm <2 > above the hydrogel microsphere layer by using a sprayer, and drying for 30 minutes at room temperature.
S5, adding a standard solution, preparing a 0.5% phthalic aldehyde standard solution, dropwise adding 2 mu L of the standard solution into a self-calibration area by using a microsampler, and drying in a 37 ℃ oven for 1 hour.
S6, setting a color comparison card and an indicating element, printing an integrated color comparison card 6 on a PET film by using a screen printing technology by using anti-fading ink, printing a two-dimensional code beside a result display area by using a micro-jet printing technology by using a photochromic ink, and curing for 10 seconds under 365nm ultraviolet light.
S7, assembling each layer, placing the bottom layer 1 (PET film with the thickness of 0.1 mm) on a clean workbench, accurately pasting the treated PVDF film to the bottom layer 1, and pasting a water-absorbing layer 5 to the sample input area to modify cellulose with the thickness of 0.5mm.
S8, covering a protective layer, cutting a PE protective film, wherein the size of the PE protective film is slightly larger than that of the test paper, covering the PE film on the surface of the test paper, and lightly pressing to ensure the fitting, and making an easy-to-tear pull piece design at one end of the protective layer.
And S9, freeze drying, namely placing the assembled test paper on a tray of a freeze dryer, setting parameters of-50 ℃ and 0.05mbar of pressure, freeze drying for 12 hours, and sealing and packaging the test paper and storing the test paper in a dark place after the test paper is finished.
The use process;
and B1, adding a sample, tearing off a protective film on the surface of the test paper, sucking 50 mu L of a phthalic dicarboxaldehyde sample to be tested by using a pipette, and dripping the sample into an input area of the test paper.
Step B2, light activation, using an ultraviolet torch with a wavelength of 365nm, irradiating for 10 seconds at a distance of 5cm from the test paper.
And step B3, waiting for the reaction, placing the test paper in the room temperature (about 25 ℃) environment, standing for 3 minutes, and waiting for the completion of the reaction.
And step B4., visually comparing, namely, observing the color change of the reaction area and the self-calibration area, comparing the color with the integrated color chart 6, and estimating the approximate concentration range of the phthalic aldehyde.
And B5., intelligently scanning, opening a special mobile phone application program, aligning the mobile phone camera to the variable color two-dimensional code, keeping the mobile phone stable, and waiting for automatic scanning and identification of the application program.
And step B6., data analysis and management, wherein an application program automatically analyzes the color change of the two-dimensional code, calculates the accurate phthalic dicarboxaldehyde concentration according to a preset algorithm, displays a concentration value and a judging result on an application program interface, and can select and store the detection result, including date, time, concentration value and other information, and the application program provides a data export function and can share or transmit the result in a CSV or PDF format.
The following main chemical reactions involved in the phthalic dicarboxaldehyde disinfectant concentration test paper and chemical formulas thereof are as follows:
reaction 1 oxidation of phthalic aldehyde (catalyzed by aldehyde dehydrogenase):
Wherein:
Represents phthalic dicarboxaldehyde
Represents phthalic acid
Is nicotinamide adenine dinucleotide (oxidized form)
NADH is nicotinamide adenine dinucleotide (reduced)
Reaction 2 reduction of NADH with NBT (tetranitrotetrazolium blue):
NADH+NBT (oxidized, yellow) - +NBT (reduction type, blue-violet)
Here the reduction of NBT forms a bluish violet formazan.
Reaction 3 photoisomerization of a photosensitive enzyme inhibitor (exemplified by azobenzene derivatives):
azobenzene (trans, inhibitory Activity) + with reduced toxicity Azobenzene (cis, no inhibitory activity)
Wherein the method comprises the steps ofRepresenting photon energy.
Reaction 4 polymerization of hydrogel microspheres:
Wherein:
Is N-isopropyl acrylamide (NIPAM)
N, N' -methylenebisacrylamide (crosslinker)
Reaction 5 surface modification reaction of the gold nanoparticles:
Wherein HS-R represents a mercapto compound and R is an organic group.
The chemical reactions together form the working principle of the phthalic aldehyde disinfectant concentration detection test paper, and high-sensitivity and specificity detection is realized. Reactions 1 and 2 are core detection reactions, reaction 3 controls the initiation of detection, and reactions 4 and 5 occur during the preparation process to construct the intelligent response material required for detection.
This example details the overall process from preparation to use of the test strip for detecting the concentration of phthalic aldehyde disinfectant. By precisely controlling each step, high quality and stability of the test paper is ensured. Meanwhile, the simplified use flow and intelligent result analysis enable the detection method to be suitable for professionals and convenient for common users to use. The test paper has wide application prospect in the fields of medical health, food safety, environmental monitoring and the like.
The present application is not limited to the above-described embodiments, which are adopted in connection with the actual demands, and various changes made by the person skilled in the art without departing from the spirit of the present application are still within the scope of the present application.
Claims (3)
1. The test paper for detecting the concentration of the o-phthalaldehyde disinfectant is characterized by comprising a bottom layer (1) made of a hydrophobic material;
The microfluidic layer (2) is arranged on the bottom layer (1), and the microfluidic layer (2) is divided into a plurality of functional areas by a hydrophobic boundary, wherein the functional areas comprise a sample input area, a reaction area, a self-calibration area and a result display area;
The intelligent response detection layer (3) is arranged on the microfluidic layer (2), and the intelligent response detection layer (3) comprises temperature response hydrogel microspheres and a signal enhancer, wherein the intelligent response detection layer (3) is arranged above a reaction area of the microfluidic layer (2);
a self-calibration layer (4) arranged on the micro-fluidic layer (2), wherein the self-calibration layer (4) is arranged above the self-calibration region of the micro-fluidic layer (2)
The sample input area is positioned at one end of the test paper and is provided with a water absorption layer (5);
the result display area is positioned at the other end of the test paper and is provided with an integrated color card (6);
The variable color indicating element is arranged above the intelligent response detection layer (3), the self-calibration layer (4) and the integrated color chart (6);
The bottom layer (1) is made of at least one material selected from polyethylene terephthalate (PET), polypropylene (PP) or Polyethylene (PE);
the microfluidic layer (2) is made of at least one hydrophilic porous membrane material selected from polyvinylidene fluoride (PVDF) membrane, nitrocellulose membrane or cellulose acetate membrane;
the hydrophobic boundary is formed of at least one material of wax, polydimethylsiloxane (PDMS), or a photo-curable resin;
the temperature response hydrogel microsphere is prepared by copolymerizing N-isopropyl acrylamide (NIPAM) and at least one cross-linking agent of N, N '-methylene bisacrylamide or N, N' -ethylene bisacrylamide;
The temperature-responsive hydrogel microsphere is encapsulated with functionalized nano gold particles with the particle size of 5-100nm, and the surfaces of the functionalized nano gold particles are modified with sulfhydryl compounds;
the aldehyde dehydrogenase is selected from aldehyde dehydrogenases of bacterial or fungal origin;
The coenzyme is nicotinamide adenine dinucleotide (NAD+) or reduced form (NADH) thereof;
the electron acceptor is selected from at least one of tetrazolium blue (NBT), dichloroindophenol (DCIP) or methylene blue;
the photosensitive enzyme inhibitor is selected from at least one of azobenzene derivative, spiropyran derivative or diarylethene derivative;
the signal enhancer is at least one of graphene quantum dots, carbon quantum dots or semiconductor quantum dots;
when the signal enhancer is graphene quantum dots, the particle size of the signal enhancer is 1-20nm, and the emission wavelength is 400-700nm;
The self-calibration layer (4) comprises a preset phthalic aldehyde solution with known concentration, and the concentration range of the phthalic aldehyde solution is 0.1% -1.0%;
the water absorption layer (5) is made of at least one material selected from modified cellulose, glass fiber or polyester fiber;
the integrated color chart (6) comprises a plurality of color areas, and each color area corresponds to a preset phthalic aldehyde concentration range;
The color-changeable indicating element is a two-dimensional code or a bar code printed by using ink which can be changed along with the concentration of the phthalic aldehyde;
The test paper also comprises a strippable transparent protective layer which is covered on the surface of the test paper, wherein the protective layer is made of at least one material selected from polyethylene, polypropylene or polyethylene terephthalate.
2. A preparation method of the phthalic aldehyde disinfectant concentration detection test paper applied to the method in claim 1 is characterized by comprising the following steps of;
s1, creating a microfluidic channel on a hydrophilic porous membrane by using a hydrophobic material printing technology;
S2, preparing temperature-responsive hydrogel microspheres containing functionalized nano-gold particles, an enzyme system and a photosensitive enzyme inhibitor;
S3, coating the hydrogel microsphere suspension liquid on a reaction area;
s4, applying a signal enhancer on the hydrogel microsphere layer;
s5, adding a phthalic aldehyde standard solution with known concentration in a self-calibration area;
S6, setting an integrated color chart (6) and a variable color indicating element;
s7, assembling the bottom layer (1), the treated hydrophilic porous membrane and the water absorption layer (5);
S8, covering a protective layer;
s9, treating the coated test paper by using a freeze-drying technology, wherein the freeze-drying is carried out at a temperature of between 40 ℃ below zero and 60 ℃ below zero and at a pressure of between 0.01 and 0.1mbar for 6 to 24 hours;
The method for preparing the hydrogel microsphere in the step S2 comprises the following steps:
M1, dispersing N-isopropyl acrylamide monomer, a cross-linking agent, functionalized nano gold particles, an enzyme system and a photosensitive enzyme inhibitor in an oil phase in the presence of a water-oil surfactant;
M2, under the protection of nitrogen, adding an initiator to perform polymerization reaction;
and M3, centrifugally separating to obtain hydrogel microspheres, and washing with deionized water.
3. The application method of the phthalic aldehyde disinfectant concentration detection test paper is characterized by comprising the following steps of;
b1, adding a phthalic aldehyde sample to be detected into a sample input area;
B2, irradiating the test paper for 5-15 seconds by using light with the wavelength of 350-400nm so as to activate detection reaction;
b3, waiting for 30 seconds to 5 minutes at 15-30 ℃ to complete the reaction;
B4, observing the color changes of the reaction area and the self-calibration area, and comparing the color with an integrated color chart (6);
b5, using intelligent equipment to scan the variable color indicating element to obtain quantitative result
And B6, analyzing the color change of the variable color indicating element by using a special application program on the intelligent equipment, calculating the accurate concentration of the phthalic aldehyde, and recording, storing and transmitting the test result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202411283006.4A CN118858270B (en) | 2024-09-13 | 2024-09-13 | Test paper for detecting concentration of phthalic dicarboxaldehyde disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202411283006.4A CN118858270B (en) | 2024-09-13 | 2024-09-13 | Test paper for detecting concentration of phthalic dicarboxaldehyde disinfectant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN118858270A CN118858270A (en) | 2024-10-29 |
| CN118858270B true CN118858270B (en) | 2024-12-20 |
Family
ID=93165773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202411283006.4A Active CN118858270B (en) | 2024-09-13 | 2024-09-13 | Test paper for detecting concentration of phthalic dicarboxaldehyde disinfectant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118858270B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104677904A (en) * | 2015-02-04 | 2015-06-03 | 北京红辉力上科技有限公司 | Test paper for testing concentration of ortho-phthalaldehyde disinfectant |
| CN105548166A (en) * | 2015-12-30 | 2016-05-04 | 广州市医德生物科技有限责任公司 | OPA (o-phthalaldehyde) disinfector concentration range detection test paper |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4273556A (en) * | 1979-12-19 | 1981-06-16 | Sherwood Medical Industries Inc. | Determination of urea |
| DE69635717T2 (en) * | 1995-10-30 | 2006-08-31 | Arkray, Inc. | Method for determining an analyte and device therefor |
| CA2998587C (en) * | 2015-09-14 | 2023-01-10 | Essenlix Corp. | Device and system for analyzing a sample, particularly blood, as well as methods of using the same |
| CN106970075B (en) * | 2017-03-20 | 2019-05-10 | 广西庆荣卫生消毒制品有限公司 | A kind of o-phthalaldehyde Concentration Testing test paper and preparation method thereof |
| CN106932391A (en) * | 2017-03-30 | 2017-07-07 | 中南林业科技大学 | A kind of Test paper based on photosensitive mesoporous silicon substrate molecular engram microsphere and preparation method thereof |
| CN107024473B (en) * | 2017-05-15 | 2019-06-25 | 北京长江脉医药科技有限责任公司 | A kind of O-phthalaldehyde disinfectant concentration quick detection test paper and the preparation method and application thereof |
| CN106970078B (en) * | 2017-05-15 | 2019-10-08 | 北京长江脉医药科技有限责任公司 | A kind of test paper detecting o-phthalaldehyde and its preparation and detection method |
| CN111504995B (en) * | 2020-05-13 | 2021-10-12 | 暨南大学 | A method for detecting phospholipase A2 based on colorimetric principle and its application |
| CN115656161A (en) * | 2022-10-25 | 2023-01-31 | 利康医药科技江苏有限公司 | Phthalic dicarboxaldehyde disinfectant concentration detection indicator card and preparation method thereof |
| CN118489681B (en) * | 2024-04-25 | 2025-02-21 | 浙江医之友环境科技有限公司 | A preparation method of o-phthalaldehyde disinfectant with concentration indication function |
-
2024
- 2024-09-13 CN CN202411283006.4A patent/CN118858270B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104677904A (en) * | 2015-02-04 | 2015-06-03 | 北京红辉力上科技有限公司 | Test paper for testing concentration of ortho-phthalaldehyde disinfectant |
| CN105548166A (en) * | 2015-12-30 | 2016-05-04 | 广州市医德生物科技有限责任公司 | OPA (o-phthalaldehyde) disinfector concentration range detection test paper |
Also Published As
| Publication number | Publication date |
|---|---|
| CN118858270A (en) | 2024-10-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gruber et al. | Integration and application of optical chemical sensors in microbioreactors | |
| JP5118288B2 (en) | Method for producing polymer layer | |
| US6984307B2 (en) | Dual glucose-hydroxybutyrate analytical sensors | |
| AU659525B2 (en) | Improved oxidative coupling dye for spectrophotometric quantitative analysis of analytes | |
| Kim et al. | Radical polymerization reactions for amplified biodetection signals | |
| US20230265482A1 (en) | Trypsin detection film, preparation method therefor, application thereof, and trypsin detection kit | |
| JP2005528896A5 (en) | ||
| US20090264308A1 (en) | biosensor solid substrate with integrated temperature control and a method to make the same | |
| CN104624259A (en) | Preparation and application of simple high-throughput microfluidic chemiluminescence paper chip | |
| EP1445329A2 (en) | Method and test strip for determining glucose in blood | |
| Zhang et al. | Based microfluidic sensors for onsite environmental detection: A critical review | |
| CN105344391B (en) | A kind of cloth chip gravity/capillary flow chemiluminescence method | |
| TWI627403B (en) | Biochemical test chip | |
| CN118858270B (en) | Test paper for detecting concentration of phthalic dicarboxaldehyde disinfectant | |
| Sobhanie et al. | Microfluidic systems with amperometric and voltammetric detection and paper-based sensors and biosensors | |
| US20070166721A1 (en) | Fluidic circuits, methods and apparatus for use of whole blood samples in colorimetric assays | |
| Benhabib et al. | Low-cost assays in paper-based microfluidic biomedical devices | |
| Witkowska Nery | Analysis of Glucose, Cholesterol and uric acid | |
| Resmi et al. | Paper based micro/nanofluidics devices for biomedical applications | |
| Gao et al. | Sensor application of poly (ethylene glycol) diacrylate hydrogels chemically-anchored on polymer surface | |
| Yan | Design of Paper-Based Analytical Devices | |
| Hasanzadeh et al. | Microfluidic paper-based devices | |
| Handa et al. | Microfluidic Paper-Based Analytical Devices for Glucose Detection | |
| Hasanzadeha et al. | Microfluidic paper-based | |
| Ruecha et al. | 3.1 What Is a Paper-Based (Bio) Chemical Sensor? |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |