CN1188406C - Cattail pollen extract and preparation process and use thereof - Google Patents

Abstract

The present invention relates to the technical field of medicine, which relates to a cattail pollen extract, a preparation method thereof and a new purpose of using the cattail pollen extract for preventing and treating myocardial ischemic cardiovascular diseases. Cattail pollen is thermally extracted by aqueous ethanol, the cattail pollen extract is obtained by a macroporous resin adsorption chromatography, and the total flavonoid content of the cattail pollen extract is 50%. Through animal experiments, the obtained cattail pollen extract can obviously lower the degree of myocardial ischemia after the ligating coronary artery, and the range of myocardial ischemia can be reduced; the obtained cattail pollen extract can obviously reduce the infarction range, and can obviously lower the increase degree of serum lactic acid and creatine kinase after the ligating coronary artery; the obtained cattail pollen extract can obviously reduce the myocardial oxygen consumption, can obviously lower the myocardial oxygen uptake rate, and can increase the coronary blood flow. Therefore, the cattail pollen extract can be used for preparing medicines and food used for preventing and treating myocardial ischemic cardiovascular diseases.

Description

Puhuang extract and its preparation method and use

Technical field:

The invention relates to the technical field of medicine, and relates to an extract of Puhuang and a preparation method thereof and the use of the extract of Puhuang in preparing medicine or food for preventing and treating myocardial ischemic cardiovascular disease.

Background technique:

Cattail is a commonly used medicinal material, derived from the pollen of various plants such as Typha L. Typha angustifolia L., Typha angustata Bory et Chaub, Typha angustata Bory et Chaub and T. latifolia L. of the family Typhaceae. Alias cattail pollen, cattail yellow and so on. Its medicinal use has a long history, and was listed as top grade in "Shen Nong's Materia Medica" in the Han Dynasty. It has the effects of stopping bleeding, removing blood stasis and treating stranguria. For vomiting blood, epistaxis, hemoptysis, uterine bleeding, traumatic hemorrhage, amenorrhea and dredging menstruation, epigastric pain, tumbling and swelling pain, bloody stranguria and astringent pain. "Compendium of Materia Medica" records: "Puhuang's hands and feet Jueyin blood is also divided into medicines, so it can cure blood and relieve pain. It can work when it is raw, and it can stop when it is cooked. When used together with Wulingzhi, it can cure all kinds of pain in the heart." The syndromes of "chest and spleen" and "true pain" described in traditional Chinese medicine are similar to coronary heart disease angina pectoris. According to clinical reports, the flavored "Shixiaosan" mainly composed of Puhuang and Wulingzhi has a certain curative effect on coronary heart disease. In addition, in recent years, studies have found that single herb Puhuang can improve microcirculation, prevent experimental atherosclerosis (AS) in rabbits, reduce blood lipids and reduce AS plaques, and can reduce serum cholesterol in patients with hyperlipidemia. Level, reduce the platelet adhesion rate and aggregation rate and improve the clinical symptoms of coronary heart disease, and less toxic side effects, safe to use. Puhuang mainly contains steroids, flavonoids, long-chain aliphatic hydrocarbons, amino acids, inorganic components, etc. : 587; 3. Thrombosis Research, 1990, 57(6): 957; 4. Chinese herbal medicine, 1998, 27(11): 693). So far, there is no report about the prevention and treatment of myocardial ischemic cardiovascular disease with Puhuang extract.

Invention content:

The invention provides an extract of Pollen Pollen and a preparation method thereof.

The preparation method of the present invention is as follows:

After heat-extracting Puhuang with water-containing ethanol, concentrate the extract, centrifuge or filter the concentrate, and take the centrifuged supernatant or filtrate; absorb the centrifuged supernatant or filtrate with macroporous resin, rinse with water to remove impurities, and then use Elute with water-containing lower alcohol; concentrate the eluent of lower alcohol to a certain volume, precipitate with ethanol, first remove the precipitate, then concentrate to dryness or remove ethanol and then spray dry to obtain the extract of Puhuang.

[Analysis of Chemical Components of Puhuang Extract]

Five compounds were obtained from the extract of Puhuang through silica gel, sephadexLH-20, ODS and other reversed-phase chromatography supports: 1. Quercetin-3,3′-dimethyl ether; 2. Quercetin-3,3′- Dimethyl ether-4'-O-β-D-glucopyranoside; 3. Isorhamnetin-3-O-β-D-galactopyranoside; 4. Isorhamnetin-3-O-new orange glucoside; 5, Typha new glycosides. Determined by chemical and spectral methods, the general chemical structure formulas of the first three compounds are as follows:

in:

When R ₁ =CH ₃ , R ₂ =H, R ₃ =CH ₃ , it is quercetin-3,3'-dimethyl ether;

When R ₁ =CH ₃ , R ₂ =Glu (glucose), R ₃ =CH ₃ , it is quercetin-3,3'-dimethyl ether-4'-O-β--D-glucopyranose Glycoside

When R ₁ =Gala (galactose), R ₂ =H, R ₃ =CH ₃ , it is isorhamnetin-3-O-β-D-galactoside

The chemical structural formulas of the remaining 2 compounds are:

Isorhamnetin-3-O-neohesperidoside Typhaloside

It can be seen that the extract of Puhuang is a mixture of flavonoids. Quantitative analysis by HPLC shows that it mainly contains isorhamnetin-3-O-β-D-galactoside, isorhamnetin-3-O-neohesperidoside, and cattail glycoside, and the content of the three accounts for the total More than 40% of the extract. Determined by ultraviolet spectrophotometry, the total flavonoid content accounts for more than 50% of the total extract. According to animal experiments, Puhuang extract can effectively prevent and treat myocardial ischemic cardiovascular disease.

Description of drawings:

Figure 1 is a graph showing the effect of Puhuang extract on the sum of the ST segments (∑ST) of the epicardial electrogram in dogs with coronary artery ligation

Figure 2 is a graph showing the effect of Puhuang extract on the range of myocardial infarction (N-ST) in dogs with coronary artery ligation

Figure 3 is a graph showing the effect of Puhuang extract on the oxygen consumption (ml/min.100g ^-1 ) of canine myocardium

Fig. 4 is the curve graph of the effect of Puhuang extract on the oxygen uptake rate (%) of canine myocardium

Figure 5 is a graph showing the effect of Puhuang extract on canine coronary flow (ml/min)

Detailed ways:

Preparation of Puhuang extract: Thermally extract Puhuang with 40-95% ethanol twice, and the amount of solvent used each time is about 4-12 times the amount of crude drug; the combined extracts are concentrated, and the concentrated solution is centrifuged or filtered; the supernatant The liquid or filtrate is adsorbed by the macroporous resin, which is a polystyrene-type porous adsorption resin with styrene as a bridging material, such as D101, D201, ZTC-1, AB-8, 1300-1 macroporous Resins, etc., first wash with water to remove impurities; then elute with water-containing lower alcohols, the lower alcohols are C ₁ ~ C ₅ alcohols such as methanol, ethanol or propanol, the concentration is 40 ~ 95%, and the dosage is 5% of the crude drug amount. ～15 times; collect the lower alcohol eluate, concentrate and precipitate with ethanol, so that the final concentration of ethanol is 70-95%; the supernatant after alcohol precipitation is concentrated to dryness, and the Pollen extract of the present invention is obtained, which is dried and pulverized It is used for later use, and the yield of the finished product is more than 1.0% of the crude drug amount.

Example 1

Put 100 kg of Puhuang medicinal materials into the extraction tank, soak in 100 liters of 70% ethanol at room temperature for 24 hours, boil and extract 3 times with 800 liters of 70% ethanol at 90-100 ° C, each extraction time is 2 hours, and combine the 3 extractions liquid, concentrated to 50 liters, filtered, and the filtrate was adjusted to 100 liters with distilled water. Slowly pass through the adsorption column that 200 liters of ZTC-1 type macroporous resin (produced by Tianjin Zhengtiancheng Clarification Technology Co., Ltd.) is housed again, remove impurity with 1000 liters of distilled water rinses; Remove liquid, concentrate to 10 liters, slowly add 100 liters of 95% ethanol under stirring, after adding, let it stand for 12 hours, centrifuge to discard the precipitate, concentrate the supernatant to dryness, further dry and pulverize to obtain yellow powder The yellow extract is 1.8 kg, and the yield is 1.8% based on crude drug amount.

Bioactivity assay

An anesthetized canine myocardial infarction model was used to observe the therapeutic effect of the Puhuang extract of the present invention on the acute myocardial infarction model and the influence of myocardial oxygen consumption. The specific experimental method is as follows:

1. Therapeutic effect of Puhuang extract on the model of acute myocardial infarction caused by coronary artery ligation in anesthetized dogs

1 Materials and methods

1.1 Animals

Healthy mongrel dogs were provided by the Animal Center of the Second Military Medical University. Both male and female. Weight 10-14kg. 18 dogs were randomly divided into 3 groups, 6 dogs in each group.

1.2 Dose setting Grouping and dosage are shown in Table 1.

                Table 1 Experimental grouping and dosing regimen

Groups Route of Administration Dose Number of Animals (Pieces)

Normal saline control group Orally, single administration 1ml/kg 6

Danshen tablet group Oral administration, single administration 1 tablet/kg 6

Puhuang extract group Oral administration, single administration 8mg/kg 6

1.3 Method

Canine acute myocardial ischemia model replication and epicardial electrogram measurement refer to the methodology of pharmacological experiments [Edited by Xu Shuyun et al. Pharmacological Experimental Methodology (Third Edition). Eighth: Cardiovascular System Drug Experimental Method. Beijing: People's Medical Publishing House, 2002: 1054]. "Multi-point fixed" epicardial electrodes were placed on the surface of the dog model heart and fixed, and the epicardial electrodes at 30 points were mapped. The signal was connected to the real-time recording computer through the PowerLab data recording and analysis system (ML785/8S, AD Instruments, Australia), and the total number of leads and the ST segment elevation value of the epicardial electrogram at different times were calculated. , in order to understand the degree and scope of myocardial ischemia.

Three hours after ligation, the heart was weighed and stained. The infarcted area was not colored, and the non-infarcted area was colored black. The colored part was cut off, and the uncolored infarcted area was weighed. Before coronary artery ligation and 3 hours after administration, 3ml of blood was collected from the right ventricle, and serum was taken to measure creatine, creatinase, CK and lactate LDH.

The test results are based on x ± S means, using SPSS statistical software for analysis of variance, comparing the significance of the difference between the groups.

2 results

2.1 The results of the effect on the degree of myocardial ischemia (epicardial electrogram ∑ST) are shown in Table 2 and Figure 1

                                                                     

(mV, x±s, n=6/group)

After administration

group administration

Dosage Index

Before 15min 30min 60min 90min 120min 180min

Birth 1 x±s 220.3 238.3± 269.0± 289.3± 301.2± 274.7± 259.0±

Rationale ml/kg ±65.7 54.2 44.1 49.4 49.5 84.2 67.5

Salt

water

% Change 10.5±10.1 27.4±24.4 36.0±19.5 43.7±31.5 26.2±21.3 19.9±19.1

Dan 1 tablet x±s 221.8 176.8± 146.0± 222.7±90.2 242.8± 242.7± 227.8±

Reference /kg ±61.1 64.5 39.5 ^*** 110.1 122.4 106.0

piece

% Change -22.2± -33.5± -1.9± 7.6±30.0 5.7±30.6 0.2±26.5

9.2 ^*** 8.6 ^*** 20.2 ^***

Po 8mg/k x±s 207.8 137.2± 140.4± 148.8± 149.8± 143.0±39.8 144.4±36.7

Yellow g ±29.5 66.2 46.4 ^*** 37.9 ^** 28.9 ^**

% Change -35.4± -33.7± -29.1± -27.6± -30.4± 8.9

24.5 ^*** 13.8 ^*** 11.1 ^*** 12.4 ^*** 19.6 ^*** 20.7 ^***

^** P＜0.05, ^*** P＜0.01 compared with normal saline group

It can be seen from Table 2 and Figure 1 that 15 minutes after coronary artery ligation, the sum of the ST segments (∑ST) of the electrocardiogram in the normal saline control group increased significantly, and the ∑ST increased to 301.2±49.5 at 90 minutes (min), and thereafter slow down; Puhuang extract group can reduce the degree of myocardial ischemia after 8mg/kg intragastric administration, ΣST decreased significantly, and the effect lasted from 15 minutes to 180 minutes after administration, and there were significant differences at each time point compared with the normal saline group Difference (P<0.01). In the positive control Danshen Tablets group, ΣST decreased from 15 minutes to 60 minutes after administration, which was significantly different from that of the normal saline group at the same time point (P<0.01), but there was no significant difference from the normal saline group after 60 minutes to 180 minutes .

2.2 The effect on the extent of myocardial ischemia (N-ST epicardial electrogram), the results are shown in Table 3 and Figure 2

                  Table 3 The effect of Puhuang extract on the size of myocardial infarction (N-ST) in dogs with coronary artery ligation

(Points, x±s, n=6/group)

                                 

15min before dose index 30min 60min 90min 120min 180min 1 x±s 27.3± 28.0±1.8 29.2±1.0 29.7±0.5 29.5±0.8 27.8±2.4 26.8±2.7 theoretical ml/kg 1.8 Salt change% 2.5±3.0 7.0±6.2 8.9 ±7.0 8.2±5.3 1.8±4.4 -1.9±6.0 Shuidan 1 x±s 28.0± 24.7±4.5 22.8± 23.8±4.7 ^** 25.8±4.7 25.3±4.1 24.5±3.8 Tablets/kg 1.7 2.9 ^** Table change% -12.0±13.7 -18.5± -15.0± -7.5±17.6 -9.3±15.3 -12.5±12.4

8.6 ^*** 15.8 ^*** Po 8mg/ x±s 28.0± 24.0±4.9 22.8± 22.7± 23.5±3.2 ^** 22.5±4.7 22.8±5.0 yellow kg 1.4 4.1 ^*** 3.6 ^***

% Change -14.7±14.4 -18.7± -19.2± -16.1± -12.4±18.

12.1 ^*** 10.6 ^*** 10.2 ^*** 17.3 ^**

^** P＜0.05, ^*** P＜0.01 Compared with the change value of the normal saline group at the same time point

It can be seen from Table 3 and Figure 2 that 8 mg/kg of Puhuang extract can significantly reduce the extent of myocardial ischemia (P<0.01), lower the N-ST value, and the effect can last for 120 minutes. The range of myocardial ischemia in the positive control drug Danshen Tablets was significantly decreased compared with the normal saline group at the same time point from 30 minutes to 60 minutes after administration (P<0.01), but there was no significant difference from the normal saline group from 90 minutes onwards. Fast and short duration of action.

2.3 Quantitative histological detection of the influence on the range of myocardial infarction, the results are shown in Table 4

      Table 4 Effect of Puhuang Extract on N-BT Staining Determination of Myocardial Infarction Range

( x±s, n=6/group)

Group Dose Infarct area/whole heart (%) Infarct area/left ventricle (%)

Normal saline 1ml/kg 12.10±2.28 23.24±1.97

Danshen tablets 1 piece/kg 9.29±0.96 ^** 17.16±3.24 ^***

Pollen Extract 8mg/kg 9.71±1.02 ^** 18.69±1.59 ^**

^** P＜0.05, ^*** P＜0.01 compared with normal saline group

It can be seen from Table 4 that oral administration of Puhuang extract at 8 mg/kg can significantly reduce the extent of myocardial infarction. The ratio of infarct area/whole heart and infarct area/left ventricle of Puhuang extract decreased significantly compared with the normal saline group (P<0.01). Both the positive control group and the Puhuang extract group could significantly reduce the size of myocardial infarction (P<0.01).

2.4 The effect of Puhuang extract on serum biochemical indicators after coronary artery ligation, the results are shown in Table 5

     Table 5 Effect of Puhuang Extract on Serum Biochemical Indexes of Right Ventricle in Dogs with Coronary Artery Ligation

( x±s, n=6/group)

Item Time Physiological Saline 1ml/kg Danshen Tablet 1 Tablet/kg Puhuang Extract 8mg/kg

CK(U/I) Before administration 149.76±15.14 149.76±20.95 125.47±11.16

After administration 615.22±45.51 ^ΔΔΔ 392.5 1±57.73 ^***ΔΔΔ 442.53±50.85 ^***ΔΔΔ

LDH before administration 367.98±68.45 404.39±47.48 436.85±98.37

(IU/L) After administration 959.21±109.21 ^ΔΔΔ 776.67±68.13 ^***ΔΔΔ 807.46±132.87 ^**ΔΔΔ

^** P<0.05, ^*** P<0.01 compared with normal saline group; ^ΔΔ P<0.05, ^ΔΔΔ P<0.01 compared with before administration

It can be seen from Table 5 that serum creatine creatinase CK and lactate LDH in each group increased after coronary artery ligation. Puhuang extract 8mg/kg and the positive control drug Danshen Tablets can both significantly reduce the elevation of serum creatine kinase (P<0.01); Puhuang extract and the positive control drug can also significantly reduce the serum level of right ventricular arterial blood Increased degree of lactic acid (P<0.01).

The experimental results show that oral administration of Puhuang extract can significantly reduce the degree of myocardial ischemia in dogs with coronary artery ligation, and narrow the scope of myocardial ischemia. Compared with the control group, the infarction area was significantly reduced, which proves that oral administration of Puhuang extract has a significant therapeutic effect on acute myocardial infarction caused by canine coronary artery ligation. The experimental results also show that Puhuang extract can significantly reduce the incidence of coronary artery ligation The elevated levels of serum lactic acid and creatine kinase suggest that it can reduce the overflow of CK and lactic acid in acute ischemic cardiomyocytes, reduce cell damage during myocardial ischemia, and have a protective effect on cardiomyocytes.

2. Effect of Puhuang Extract on Myocardial Oxygen Consumption in Anesthetized Dogs

1 Materials and methods

1.1 Animals

Healthy mongrel dogs were provided by the Animal Center of the Second Military Medical University. Both male and female. 11~13kg. 15 pieces were randomly divided into 3 groups, 5 pieces in each group.

1.2 See Table 6 for dose settings

                                                      

Groups Route of Administration Dose Number of Animals (Pieces)

Normal saline control group Orally, single administration 1ml/kg 5

Seabuckthorn total flavonoids group Oral, single administration 24mg/kg 5

Puhuang extract group Oral administration, single dose 8mg/kg 5

1.3 Test method

Refer to Xu Shuyun's method (Xu Shuyun et al., editor-in-chief. Pharmacological Experimental Methodology (Third Edition). Chapter 8: Cardiovascular System Drug Experimental Method. Beijing: People's Medical Publishing House, 2002: 1054). The dog was anesthetized, the right femoral artery was isolated, and arterial blood gas analysis was prepared. Expose the heart, make a pericardial bed, free the root of the ascending aorta and the upper part of the left anterior descending coronary artery, and place electromagnetic flowmeter probes (MFV-1100/1200, Nihon Kohden, Japan) with appropriate inner diameters to measure coronary flow. The right jugular vein was separated, and the cardiac catheter was inserted into the coronary sinus, cardiac catheterization. Simultaneously extract coronary sinus and femoral artery blood samples (0.5% heparin anticoagulant), measure pO ₂ and pH with a blood gas analyzer (DH-1830 blood gas acid-base analyzer, Nanjing Analytical Instrument Factory), and convert them into arterial and venous blood oxygen content. At the end of the experiment, the heart was weighed, and 10-15ml of 10% Shanghai advanced carbon ink was perfused countercurrently in the area below the measurement site of the electromagnetic flowmeter, and the black stained area was cut off and weighed to calculate the myocardial oxygen consumption. The formula is: myocardial oxygen consumption (ml/min/100g)=coronary blood flow ml/min/100g×(arterial blood oxygen ml%-coronary sinus blood oxygen ml%).

Experimental data with x ± s means, using SPSS 10.0 software for analysis of variance, for significance testing.

2 results

See Table 7 and Figure 3 for the effect of Puhuang extract on the oxygen consumption of canine myocardium

Table 7 Effect of Puhuang extract on canine myocardial oxygen consumption (mL/min.100g ^-1 ) (x±s, n=5)

After giving medicine

Group Index Index Before administration

|

Normal saline x±S 204.6±9.3 210.1±9.4 205.2±5.4 205.2±5.5 212.8±4.8

1ml/kg Change % 2.8±5.5 0.4±4.2 0.4±3.1 4.2±4.9

Sea buckthorn total flavonoids x±S 213.7±5.8 191.5±10.7 ^ΔΔΔ 186.4±9.4 ^ΔΔΔ 188.2±9.4 ^ΔΔΔ 189.9±8.5 ^ΔΔ

24mg/kg Change % -10.4±4.4 ^*** -12.7±3.8 ^*** -12.0±2.2 ^*** -11.1±3.6 ^***

Pollen extract x±S 214.0±10.0 183.7±12.2 ^ΔΔ 179.3±9.1 ^ΔΔΔ 182.7±12.3 ^ΔΔ 191.5±14.0

8mg/kg Change % -14.2±3.1 ^*** -16.1±4.9 ^*** -14.4±8.3 ^*** -10.3±7.7 ^***

^ΔΔ P<0.05, ^ΔΔΔ P<0.01 compared with before administration; ^** P<0.05, ^*** P<0.01 compared with normal saline group

From Table 7 and Figure 3, it can be seen that the myocardial oxygen consumption decreased compared with the normal saline group after oral administration of Puhuang extract at 8 mg/kg, and the change rate of myocardial oxygen consumption at each time point from 30 minutes to 120 minutes Compared with normal saline group, the difference was significant (P<0.01). The total flavonoids of seabuckthorn can significantly reduce myocardial oxygen consumption, and the reduction rate is significantly different from that of the normal saline group at the same time point at 30-120 minutes (P<0.01).

The effect of Puhuang extract on the oxygen uptake rate of canine myocardium, see Table 8 and Figure 4

     Table 8 The effect of Puhuang extract on the oxygen uptake rate (%) of canine myocardium ( x±s, n=5)

After administration

Group Index Index Before administration

|

Normal saline x±S 84.6±3.7 86.9±2.6 85.6±1.6 86.8±2.2 89.4±2.5

1ml/kg % change 2.9±4.6 1.3±2.9 2.7±2.6 5.7±2.3

Seabuckthorn total flavonoids x±S 79.8±5.3 66.2±5.6 65.1±6.1 65.7±2.9 68.5±5.6

24mg/kg Change% -17.1±4.2 ^*** -18.5±4.2 ^*** -17.5±2.0 ^*** -14.2±3.1 ^***

Pollen extract x±S 79.6±7.6 62.9±7.2 ^ΔΔ 63.5±7.1 ^ΔΔ 66.2±6.8 70.7±6.9

8mg/kg Change % -21.1±3.0 ^*** -20.3±3.7 ^*** -16.6±7.5 ^*** -10.9±7.1 ^***

^ΔΔ P<0.05, ^ΔΔΔ P<0.01 compared with before administration; ^** P<0.05, ^*** P<0.01 compared with normal saline group

It can be seen from Table 8 and Figure 4 that Puhuang extract can significantly reduce the myocardial oxygen uptake rate after intragastric administration; compared with the same time point in the normal saline group, the reduction rate is very significant at each time point from 30 minutes to 120 minutes (P<0.01), the positive control drug seabuckthorn total flavonoids can significantly reduce the myocardial oxygen uptake rate.

The effect of Puhuang extract on canine coronary flow, see Table 9 and Figure 5

               Table 9 Effects of Pollen Extract on Canine Coronary Flow (ml/min) ( x±s, n=5)

After administration

Group Index Index Before administration

|

Normal saline x±S 41.4±8.6 41.4±8.3 41.2±8.4 40.8±8.7 41.0±8.2

1ml/kg Change % 0.2±2.5 -2.4±0.8 -1.6±1.4 -0.7±2.3

Seabuckthorn total flavonoids x±S 44.2±10.2 47.6±10.4 47.2±10.4 47.0±10.6 45.6±10.2

24mg/kg Change % 8.0±2.2 ^*** 7.0±2.3 ^*** 6.5±0.8 ^*** 3.3±1.4

Pollen Extract x±S 42.4±10.4 45.8±10.1 44.4±10.7 43.4±10.5 42.6±10.8

8mg/kg Change % 8.6±3.3 ^*** 4.8±2.3 ^** 2.5±1.5 0.3±2.1

^ΔΔ P<0.05 compared with before administration; ^** P<0.05, ^*** P<0.01 compared with normal saline group

It can be seen from Table 9 and Figure 5 that the Puhuang extract can increase the coronary flow rate; compared with the same time point of the normal saline group, the increase rate is significantly different at 30 and 60 minutes (P<0.05), and the positive control drug seabuckthorn total Flavonoids can also significantly increase coronary flow, and the action time is from 30-90 minutes.

It can be seen from the above that the Puhuang extract can significantly increase the coronary flow of anesthetized dogs, significantly reduce the myocardial oxygen uptake rate, and significantly reduce the myocardial oxygen consumption, so it can be used to prepare drugs or food for the prevention and treatment of myocardial ischemic cardiovascular diseases.

Claims (1)

1. the purposes of a kind of Puhuang extract in the medicine or the food of preparation prevention and treatment myocardial ischemic cardiovascular disease, described Puhuang extract is prepared by following method, comprises the steps: (1) extraction After heat-extracting Puhuang with aqueous ethanol, the extract is concentrated, the concentrate is centrifuged or filtered, and the supernatant or filtrate is taken; (2) Purification Adsorb the centrifuged supernatant or filtrate with macroporous resin, rinse with water to remove impurities, and then elute with water-containing lower alcohol; the eluate is concentrated to a certain volume, precipitated with ethanol, and then concentrated to dryness or removed Spray-dry after ethanol, get the total flavonoid content more than 50% of the Puhuang extract, said macroporous resin is polystyrene type porous adsorption resin, said water-containing lower alcohol concentration is 40%～95%, so Said lower alcohol is the alcohol of C1～C5, and said ethanol final concentration with ethanol precipitation is 70%～95%.

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