CN118773025B

CN118773025B - Talaromyces pineophilus and its application in suppressing the source of wheat fusarium rust

Info

Publication number: CN118773025B
Application number: CN202411259059.2A
Authority: CN
Inventors: 王秦虎; 田凯; 许云; 姜佳琦; 刘慧泉
Original assignee: Shenzhen Research Institute Of Northwest University Of Agriculture And Forestry Science And Technology
Current assignee: Shenzhen Research Institute Of Northwest University Of Agriculture And Forestry Science And Technology
Priority date: 2024-09-10
Filing date: 2024-09-10
Publication date: 2024-11-12
Anticipated expiration: 2044-09-10
Also published as: CN118773025A

Abstract

The invention belongs to the technical field of microorganisms, and discloses panus pinophilum and application thereof in inhibiting wheat gibberella bacteria sources. The panus pineri BJ10-11 provided by the invention has broad-spectrum inhibition effect on plant pathogenic fungi, particularly has remarkable inhibition effect on wheat scab and can effectively inhibit the formation of wheat gibberella. Therefore, the panus pinophilum BJ10-11 has wide application prospect as a biological pesticide, and provides a new solution for green prevention and control of plant diseases.

Description

Monilinia lactiflora and application thereof in inhibition of wheat gibberella pathogen source

Technical Field

The invention belongs to the technical field of microorganisms, and discloses panus pinophilum and application thereof in plant fungal disease control, in particular to application thereof in inhibition of wheat gibberella pathogen.

Background

Plant fungal diseases are a common problem in agricultural production, often resulting in a substantial decrease in crop yield and affecting its quality. Wherein, wheat scab is taken as a fungal disease, and the pathogen comprises fusarium graminearum Fusarium graminearum, fusarium asiaticum Fusarium asiaticum, fusarium yellow Fusarium culmorum, fusarium pseudograminearum Fusarium pseudograminearum and other fusarium species, and the pathogen not only directly damages the wheat ear, but also generates toxins harmful to human bodies, thereby seriously affecting the production of wheat.

At present, the prevention and treatment of most plant fungal diseases mainly depend on chemical pesticides. However, long-term use of chemical pesticides not only easily causes drug resistance of pathogenic bacteria, but also causes pollution to the environment, damages ecological balance, and affects quality and safety of agricultural products. Therefore, it is important to search for a more environmentally friendly and sustainable disease control method. The microbial control is taken as a novel biological control means, and is favored by agricultural scientific researchers due to the advantages of safety, high efficiency, no pollution and the like. The application of the microbial strain with broad-spectrum antibacterial activity in the prevention and control of plant diseases is researched by screening the microbial strain with broad-spectrum antibacterial activity, and the microbial strain becomes a research hotspot in the current prevention and control field of agricultural diseases.

The wheat head is directly harmed by the scab, and the disease process is complex and is easily influenced by various factors such as climate, soil conditions and the like, so that the research and development of the microbial control technology are difficult. The ascus shell remained in the wheat habitat is the main bacterial source for the occurrence of the scab, and the occurrence and the epidemic degree of the wheat scab are directly related to the quantity of the bacterial source in the field. Therefore, the control and cleaning of the field bacterial sources is a fundamental way to prevent the occurrence of scab from the source. Under the background, screening of microorganism strains with high-efficiency inhibition effect on gibberella is a technical problem to be solved urgently.

The genus Talaromyces (Talaromyces) belongs to the fungus kingdom (Fungi), the phylum ascomycota (Ascomycota), the class Ascomycetes (Eurotiomycetes), the order Ascomycetes (Eurotiales), the family Rhizoctonia (Trichocomaceae). The basket bacteria mainly originate from plants, soil and sea, are applied to various fields including food, environment, agricultural production and the like, have the functions of resisting bacteria, producing enzyme active substances, biologically preventing and treating and the like, and are fungi with development potential. Meanwhile, some basket bacteria have great biological control potential in the control of partial vegetable diseases.

Disclosure of Invention

In order to overcome the problems in the prior art, the invention provides the panus pinophilum and the application thereof in the inhibition of the wheat gibberella. Compared with the prior art, the invention has the advantages of providing the panus pineri which has broad-spectrum inhibition effect on pathogenic bacteria of plant fungal diseases, and particularly, the panus pineri can effectively inhibit the formation of wheat gibberella pathogen sources, thereby providing a new way for preventing and controlling plant diseases.

In one aspect, the invention relates to a panus pinophilum, the preservation information is as follows:

Strain name: BJ10-11;

classification naming: panus pinophilum Talaromyces pinophilus;

the time of collection of the preservation center: 2024, 06, 24;

preservation certificate issuance time: 2024, 07, 22;

Preservation unit: china general microbiological culture Collection center (CGMCC);

preservation number: CGMCC No.41380.

Address: the institute of microbiology, national academy of sciences, china, the area North Star, west way 1, 3, beijing, chaoyang.

Further, in the panus pinophilum provided by the invention, the ITS sequence of the panus pinophilum consists of the sequence shown in SEQ ID NO:1 and SEQ ID NO:2, and the BenA sequence is amplified by a primer pair shown in SEQ ID NO:3 and SEQ ID NO:4, and the CaM sequence is obtained by amplifying the primer pair shown in SEQ ID NO:5 and SEQ ID NO:6, amplifying the primer pair shown in the specification.

In another aspect, the invention also relates to a biocontrol agent comprising the supernatant, fermentation product, filtrate, extract or effective inhibitory fraction of the panus pineri and/or its culture.

Spores and/or mycelia of the basket-like bacteria BJ10-11, or any active variant thereof, provided herein, can be processed into a variety of forms of formulations including, but not limited to, mycelia pastes, mycelia pellets, spore suspensions, wettable particles, powders, dust, slurries, dry powders, aqueous or oily-based liquid products, and the like. These formulations comprise not only the panus pinophilum BJ10-11 or active variants thereof and/or compositions derived therefrom, but also possibly carriers and other conventional auxiliary agents.

As used herein, "supernatant" refers to the portion of liquid that remains when the panophila pinolens BJ10-11 grows in a liquid medium or is transferred from a solid medium to another liquid and the cells are removed by centrifugation, filtration, sedimentation, or other means known in the art. In some embodiments, the supernatant may be diluted with another substance, such as water, buffer, fresh medium, and/or other suitable formulation ingredients. The diluted supernatant is still considered to be the supernatant of the present invention.

As used herein, "filtrate" refers to a liquid obtained by filtering the resulting liquid through a filter membrane after fermentation culture of the panus pineri BJ10-11 in a liquid medium. The filtrate contains concentrated active bacteriostatic components compared to the fermentation culture or supernatant.

As used herein, "extract" refers to a liquid substance that is extracted from the fermentation culture of panus pinophilum BJ10-11 by a solvent (e.g., water, detergent, buffer, and/or organic solvent), and separated from the panus pinophilum BJ10-11 by centrifugation, filtration, or other solid-liquid separation means known in the art. The extract may comprise a higher concentration of said effective bacteriostatic component than the fermentation culture of the pre-extraction panaxoplia pinolens BJ 10-11. Alternatively, the filtrate or extract may be further diluted with another composition, such as water, buffer, fresh medium, and/or formulation. Such diluted filtrates or extracts are still considered to be the filtrates and extracts of the invention.

As used herein, the effective bacteriostatic component refers to enzymes, macromolecular compounds, micromolecular compounds, or byproducts produced by fermentation of the panus pinophilum BJ 10-11. The effective bacteriostatic component is a fermentation culture or the thallus itself present in the supernatant/filtrate, also contained in the panus pinophilum BJ10-11, which component, when applied in an effective amount to a target plant or its habitat, can improve disease resistance of the plant or control pathogenic bacteria causing fungal diseases of the plant.

In another aspect, the invention relates to the use of the panus pinophilum or the biocontrol agent in the prevention and treatment of plant fungal diseases, wherein the pathogenic bacteria of the fungal diseases are at least one of fusarium graminearum Fusarium graminearum, fusarium asiaticum Fusarium asiaticum, fusarium yellow Fusarium culmorum, fusarium pseudograminearum Fusarium pseudograminearum, fusarium oxysporum Fusarium oxysporum, fusarium apple tree rot germ VALSA MALI and fusarium pomace Botryospuaeria berengeriana.

Illustratively, in the applications provided herein, the fungal disease is wheat scab, wheat stem basal rot, fusarium wilt, apple rot, apple ring rot, and the like.

In another aspect, the present invention relates to a method for controlling plant fungal diseases, which is characterized in that a biocontrol agent prepared from panus pineri or a panus pineri is directly applied to pathogenic bacteria causing fungal diseases and habitat thereof;

The pathogenic bacteria of the fungal diseases are at least one of Fusarium graminearum Fusarium graminearum, fusarium asiaticum Fusarium asiaticum, fusarium yellow Fusarium culmorum, fusarium pseudograminearum Fusarium pseudograminearum, fusarium oxysporum Fusarium oxysporum, fusarium apple tree rot germ VALSA MALI and Fusarium pominum Botryospuaeria berengeriana.

Illustratively, in the methods for controlling fungal plant diseases provided herein, the panus pinpointing or the biocontrol agent is applied to plants having a gibberellic source or a potential gibberellic risk.

Illustratively, in the method for controlling plant fungal diseases provided by the invention, the pathogen of the gibberellic disease is at least one of fusarium graminearum Fusarium graminearum, fusarium asiaticum Fusarium asiaticum, fusarium yellow Fusarium culmorum, fusarium pseudograminearum Fusarium pseudograminearum and other fusarium graminearum.

Further, in the method for inhibiting bacterial sources provided by the invention, the inhibition process comprises, but is not limited to, inhibition of at least one link of pathogenic fungi bacterial source formation, hypha growth, conidium production, conidium germination and sexual reproduction.

Compared with the prior art, the technical scheme provided by the invention has at least the following beneficial effects or advantages.

The invention successfully separates and provides the panus pinophilum BJ10-11 with the capability of effectively inhibiting pathogenic fungi, and creatively provides a specific application method of the strain. The strain, or genetically modified variants, active variants thereof, and compositions derived from these strains, can be applied to habitat of plants and pathogenic fungi to control plant fungal diseases from a source, particularly wheat scab. In particular, the invention has wide and remarkable application efficacy in the prevention and control of plant fungal diseases, including but not limited to: effectively preventing the plants from being infected by pathogenic fungi for the first time, successfully treating the plants infected by the pathogenic fungi, and actively controlling the formation and the spread of pathogenic fungi sources in plant habitats.

The panus pinophilum BJ10-11, the biocontrol agent or the method disclosed by the invention have remarkable effects on controlling pathogenic bacteria of one or more plant fungal diseases. These pathogenic fungi may include, but are not limited to, the following species: fusarium graminearum Fusarium graminearum, fusarium asiaticum Fusarium asiaticum, fusarium yellow Fusarium culmorum, fusarium pseudograminearum Fusarium pseudograminearum, and the like; fusarium oxysporum Fusarium oxysporum, apple tree rot germ VALSA MALI, apple ring rot germ Botryospuaeria berengeriana and other important pathogenic fungi.

In the practice of the present invention, a key step involves the precise application of an effective amount of the panophila pinolensii BJ10-11 or an active variant thereof and/or a composition derived therefrom to a plant body, a plant habitat or a potential locus of phytopathogenic fungi. Wherein the strain BJ10-11 of Monilinia and/or the composition derived therefrom is specifically designed for controlling pathogenic fungi that can cause fungal diseases in plants. Of particular note, in particular embodiments, fungal diseases to which the present invention is directed may include, but are not limited to, the following: wheat scab, wheat stem rot, fusarium wilt, apple tree rot, apple ring rot, and the like. Likewise, pathogenic bacteria of these diseases also encompass the aforementioned listed species.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is to be understood that the drawings in the following description are only exemplary of the invention and that other similar or analogous results may be obtained from these drawings by those of ordinary skill in the art without undue burden.

FIG. 1 is a phylogenetic tree of Monilinia lactiflora BJ 10-11.

FIG. 2 is a graph showing morphological characteristics of colonies of Monilinia alternata BJ 10-11. Wherein A is the front surface of a panophila pinolephis BJ10-11 colony; b is the back of the colony of the panophilum pinophilum BJ 10-11.

FIG. 3 is a diagram showing microscopic morphological characteristics of the basket-like organism BJ 10-11. Wherein A is conidiophore; b is conidium.

FIG. 4 is a graph showing the result of culturing the basket-like bacterium BJ10-11 against Fusarium graminearum. Wherein PH-1 represents culture of Fusarium graminearum PH-1 alone, and PH-1+BJ10-11 represents culture of Fusarium graminearum PH-1 in opposition to BJ10-11 of Monascus pinophilum.

FIG. 5 is a graph showing the results of inhibiting the production of Fusarium graminearum conidium by Monophilum BJ 10-11. Wherein A is a phenotype chart of fusarium graminearum PH-1 when being singly cultured; b is a phenotype diagram of the co-culture of fusarium graminearum PH-1 and panophilum pinophilum BJ 10-11.

FIG. 6 is a graph showing the results of inhibiting germination of Fusarium graminearum conidia by Monilinia mobilis BJ 10-11. Wherein PH-1 represents the single culture of fusarium graminearum, PH-1+BJ10-11 represents the co-culture of fusarium graminearum PH-1 and panophilum BJ 10-11; 2h and 4h represent germination of Fusarium graminearum conidia after 2h and 4h of cultivation, respectively.

FIG. 7 is a graph showing the results of inhibiting fusarium graminearum exing capsule formation on straw after fusarium graminearum BJ10-11 inoculation prior to fusarium graminearum PH-1. Wherein A is PH-1, indicating that only PH-1 was inoculated; b is BJ10-11+PH-1, which means that BJ10-11 is inoculated first and PH-1 is then inoculated.

FIG. 8 is a graph showing the results of inhibiting fusarium graminearum exing capsule formation on straw after simultaneous inoculation of Monilinia graminearum BJ10-11 with fusarium graminearum PH-1. Wherein A is PH-1, indicating that only PH-1 was inoculated; b is PH-1+BJ10-11, indicating that PH-1 and BJ10-11 are inoculated simultaneously.

FIG. 9 is a graph showing the results of a field simulation test of the inhibition of fusarium graminearum cyst formation after the inoculation of the basket-like bacteria BJ10-11 prior to fusarium graminearum PH-1. Wherein A is PH-1, indicating that only PH-1 was inoculated; b is BJ10-11+PH-1, which means that BJ10-11 is inoculated first and PH-1 is then inoculated.

FIG. 10 is a graph showing the results of a field simulation test for inhibiting fusarium graminearum cyst formation after simultaneous inoculation of Monophilum pinophilum BJ10-11 with fusarium graminearum PH-1. Wherein A is PH-1, indicating that only PH-1 was inoculated; b is PH-1+BJ10-11, indicating that PH-1 and BJ10-11 are inoculated simultaneously.

Detailed Description

The following describes the technical aspects of the present invention with reference to examples, but the present invention is not limited to the following examples. The experimental methods and the detection methods in each embodiment are conventional methods unless otherwise specified; the reagents and materials are commercially available unless otherwise specified, and the percentages in the examples below are by mass unless otherwise specified.

Example 1

The embodiment provides the separation and identification result of the panus pinophilum BJ10-11, and proves the inhibition effect of the panus pinophilum on wheat scab representative pathogenic bacteria Fusarium graminearum.

Isolation of strains: and 2023, collecting soil samples from different wheat Tian Shengjing in Shandong province, hebei province, shanxi province, gansu province, yunnan province and the like, and separating microorganisms in the wheat field soil by a dilution coating flat plate method and a selective culture medium. After 5-8 d, colonies with different colors and morphologies are picked up and purified on PDA culture medium, and strains are preserved.

Screening of strains: the inhibition of the wheat scab representative pathogen Fusarium graminearum colonies by the different isolated strains was determined by plate-facing method. 3 biological replicates were set for each group, incubated at 25℃in opposition for 3-5 d, and the bacterial inhibition of the different isolates against Fusarium graminearum was calculated. And selecting a strain with higher bacteriostasis rate for subsequent experiments.

Identification of strains: in order to molecular identify these strains, DNA of candidate strains was extracted by CTAB method, and their ITS, benA, caM sequences were amplified by PCR using ITS4/ITS5, BT2a/BT2b, CMD5/CMD6 as primers (Table 1). After sequencing of the PCR products, candidate 25 strains of Brucella was initially confirmed by comparison with NCBI nucleic acid database (Table 3).

Phylogenetic analysis of BJ10-11 (FIG. 1) was performed using ITS ITS, benA and CaM sequences, and it was further confirmed that it was a Monilinia lactis.

The morphological identification result of the panophila pinnata BJ10-11 (figure 2) shows that the front center of the bacterial colony is dark green, the edge is white and the bacterial colony is wavy; the center of the back of the colony is reddish brown, and the edge is white. The aerial hyphae of the colony are short and loose and flocculent, and a large number of light green conidia are attached. Microscopic observation (FIG. 3) shows that the conidiophore is broom-shaped, and the conidiophore is round or oval, and the size is about 3.26+/-0.23 multiplied by 3.95+/-0.51 mu m.

Table 1: primers for identifying basket-like bacteria molecules

Table 2: PCR amplification procedure

Table 3: antibacterial rate of basket bacteria on growth of Fusarium graminearum

Example 2

This example demonstrates that the Monascus pinophilum BJ10-11 has broad-spectrum antibacterial effect against multiple pathogens and other important pathogenic fungi of wheat scab.

To confirm the inhibition of Fusarium graminearum colony growth by Monophilum BJ10-11, a punch with a diameter of 7 mm was used to punch out the cakes at the edges of the colonies of BJ10-11 and PH-1, and the cakes were inoculated on PDA medium for counter culture, and after culturing at a distance of 4 cm and at 25℃in the dark for 5 d, the cakes were observed. As shown in FIG. 4, the colony of the Monascus pinophilum BJ10-11 can be rapidly transmitted to different positions on the dish, and has a remarkable inhibition effect on the growth of the colony of Fusarium graminearum PH-1.

To determine whether panophila pinophilum BJ10-11 could inhibit other pathogens of wheat scab, we tested their inhibition of Fusarium Asian and Fusarium yellow using similar methods. The statistics of the antibacterial rate show that the panophila pinolepis BJ10-11 has a remarkable inhibition effect on Asian Fusarium and yellow Fusarium as well (Table 4). Thus, the Monascus pinophilum BJ10-11 can inhibit a variety of wheat scab.

To further investigate whether panophila BJ10-11 could inhibit other Fusarium species, we tested their inhibition of wheat stalk rot/scab pathogen Fusarium pseudograminearum, and Fusarium oxysporum, fusarium oxysporum. Experimental results indicate that Fusarium pinophilum BJ10-11 inhibits Fusarium species in a broad spectrum (Table 4). Similarly, we have found that Monilinia lactiflora BJ10-11 inhibits other important pathogenic fungi, such as apple tree rot and apple ring rot (Table 4). The results are consistent to show that the panus pineri BJ10-11 has broad-spectrum inhibition effect on a plurality of important plant pathogenic fungi, and can be used for preventing and treating common plant fungal diseases such as wheat scab, wheat stem basal rot, fusarium wilt, apple tree rot, apple ring rot and the like.

Table 4: bacteriostasis rate of basket-like bacteria BJ10-11 on pathogenic bacteria of common plant fungal diseases

Example 3

This example demonstrates the inhibition of F.graminearum PH-1 production by P.pinophilum BJ 10-11.

5 Bacterial cakes are respectively picked up at the edges of the bacterial colonies of the panus pinophilum BJ10-11 and the fusarium graminearum PH-1 by using a puncher with the diameter of 7mm, and are placed in a 50 mL CMC culture medium （15 g carboxymethylcellulose,1.0 g NH₄NO₃,1.0 g KH₂PO₄,0.5 g MgSO₄·7H₂O,1.0 g Yeast Extract, to be supplemented with water to 1L, sterilized at the temperature of 121 ℃ for 20 min for use), and 3 repeats are set. The control experiment is that 5 bacterial cakes of fusarium graminearum PH-1 and a blank PDA culture medium are respectively placed in a 50 mL CMC culture medium for co-culture, and 3 replicates are arranged. After shaking culture at 25℃and 180 rpm for 5d, the molecular spores were collected by centrifugation at 3500 rpm, 7, min and resuspended in 30mL of sterile water. The number of conidia was counted under a microscope using a hemocytometer, and the conidia inhibition was calculated. As shown in FIG. 5, when Fusarium graminearum PH-1 was co-cultured with Monilinia Pini BJ10-11, the spore yield of Fusarium graminearum PH-1 was significantly reduced.

Example 4

This example illustrates the inhibition of germination of fusarium graminearum PH-1 conidia by panophilum BJ 10-11.

5 Cakes were obtained from the edge of the colony of Fusarium graminearum PH-1 using a punch with a diameter of 7 mm, placed in 50mL CMC medium, collected conidia after shaking culture at 25℃and 180 rpm for 5d, resuspended in sterile water and adjusted to a concentration of 10 ⁶ spores/mL. Spore suspensions of BJ10-11 were prepared using 0.1% Tween 20 and adjusted to a concentration of 10 ⁷ spores/mL. Spore suspensions of PH-1 and BJ10-11 were aspirated into 50mL centrifuge tubes, respectively, and YEPD medium (3.0 g Yeast Extract,10.0 g peptone,20.0 g Dextrose, used after 1L of water was supplemented and sterilized at 121℃for 20 min) was added to the centrifuge tubes to 15 mL. The control group was Fusarium graminearum PH-1 spore suspension and 0.1% Tween 20. The treatment group and the control group were each provided with 3 biological replicates. After shaking 2h and 4h at 25℃and 180 rpm, germination of Fusarium graminearum conidia was observed under a microscope.

As shown in FIG. 6, when the bastard fungus BJ10-11 and Fusarium graminearum PH-1 conidia were co-cultured for 2h, none of the conidia of the treatment and control groups germinated; when 4 h is co-cultured, the germination rate of the conidia of the treatment group is extremely remarkably reduced compared with that of the control group. Experimental results show that the panus pinophilum BJ10-11 can inhibit the germination of fusarium graminearum conidium.

Example 5

This example illustrates the inhibition of the source of Alternaria wheat by Monilinia Pini BJ 10-11.

To determine whether panophila pinophilum BJ10-11 can inhibit the formation of the wheat scab source, we tested its effect on the formation of fusarium graminearum on wheat straw.

Firstly, preparing test straws: cutting mature wheat straw sections into 5 cm long sections, and autoclaving at 121 ℃ for 20: 20 min; 3 sterilized straws are placed on a 2% agar culture medium, and the sterilized straws are used after the culture medium is solidified.

Second, the effect of the application of P.pastoris BJ10-11 on the formation of fusarium graminearum on straw was evaluated by counting the formation of fusarium graminearum on straw after application. The brief method is as follows: the spore suspension concentrations of the basket-shaped bacteria BJ10-11 and the fusarium graminearum PH-1 are adjusted to 10 ⁷ and 10 ⁶ spores/mL, and 500 mu L of the spore suspension is respectively coated on the prepared straw culture medium; after 7-10 d of dark culture in a 25 ℃ incubator, adding 0.1% Tween, scraping all hyphae on the surface of the culture medium, and placing the culture medium in the 25 ℃ black light incubator for continuous culture for 1 week so as to induce the fusarium graminearum to generate ascus shells on wheat straw.

The experimental results show that a large amount of fusarium graminearum shells are attached to wheat straws in a control vessel which is not subjected to biocontrol bacteria treatment, and no ascofoles are formed on the straws in an experimental group which is subjected to pretreatment by the panophila BJ10-11 (figure 7); similarly, in the experimental group in which the panophila pinolephis BJ10-11 was simultaneously applied, a small amount of ascus shell was formed on the straw (FIG. 8).

Therefore, the bastard fungus BJ10-11 can effectively inhibit the formation of fusarium graminearum cyst on wheat straw, namely, the bastard fungus can effectively inhibit the formation of a wheat gibberella, and the bastard fungus can better inhibit the fungus source by being applied in advance.

Example 6

The embodiment is a simulation field experiment, and illustrates the inhibition effect of the panophila pinolens BJ10-11 on the gibberella wheat germ source in a simulation field environment.

To further determine whether panophila pinophilum BJ10-11 can inhibit the formation of a wheat scab source in soil, we tested its effect on the formation of fusarium graminearum sporangium on wheat straw in a simulated field environment.

Firstly, preparing test straws: cutting mature wheat straw sections into 5cm long sections, and autoclaving at 121 ℃ for 20: 20 min; 3 sterilized straws are placed in a soil matrix for standby.

Secondly, the influence of the bassinet on the formation of the wheat gibberella is evaluated and simulated in the field environment by counting the formation of fusarium graminearum on the straw after the bassinet BJ10-11 is applied. The brief method is as follows: the spore suspension concentrations of the basket-shaped bacteria BJ10-11 and the fusarium graminearum PH-1 are adjusted to 10 ⁷ and 10 ⁶ spores/mL, and 500 mu L of the spore suspension are respectively sprayed on the straws; after culturing 10 d under outdoor natural environment, adding 0.1% Tween 20, scraping all hyphae on the surface of the matrix, and culturing in a black light lamp incubator at 25deg.C for 1 week to induce Fusarium graminearum to produce ascus shells on wheat straw.

The experimental results show that a large amount of fusarium graminearum shells are attached to wheat straws in a control vessel which is not subjected to biocontrol bacteria treatment, and only a small amount of ascoforms are formed on the straws in an experimental group which is subjected to pretreatment by the panus pinophilum BJ10-11 (figure 9); similarly, in the experimental group with the simultaneous application of the panophila pinolephis BJ10-11, the ascal shells on the straw were significantly reduced (fig. 10).

The analysis of the results shows that the panus pineri BJ10-11 can still effectively inhibit the formation of fusarium graminearum on wheat straws in a simulated field test, the action effect of the panus pineri BJ10-11 is basically consistent with the result obtained in an indoor test, and meanwhile, the panus pineri can effectively inhibit the formation of a wheat gibberella source, and can be used as biocontrol bacteria for preventing and controlling wheat gibberella diseases.

As described above, the basic principles, main features and advantages of the present invention are better described. The above examples and descriptions are merely illustrative of preferred embodiments of the present invention, and the present invention is not limited to the above examples, and various changes and modifications to the technical solution of the present invention by those skilled in the art should fall within the scope of protection defined by the present invention without departing from the spirit and scope of the present invention.

Claims

1. A Talaromyces pinophilus , characterized in that the Talaromyces pinophilus is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with the deposit number being CGMCC No.41380.

2. A biocontrol agent, characterized in that it comprises the Talaromyces pineophilus according to claim 1.

3. Use of the pine-loving Talaromyces according to claim 1 or the biocontrol agent according to claim 2 in inhibiting pathogens of plant diseases, characterized in that the pathogens of plant diseases are at least one of Fusarium graminearum , Fusarium asiaticum , Fusarium culmorum , Fusarium pseudograminearum , Fusarium oxysporum , Valsa mali, and Botryospuaeria berengeriana .

4. A method for preventing and controlling wheat fusarium rust, characterized in that the method comprises applying the pine-loving Talaromyces pine-loving described in claim 1 or the biocontrol agent described in claim 2 to plants having a source of wheat fusarium rust;

The pathogen of wheat fusarium rust is Fusarium graminearum .

5. The method according to claim 4 is characterized in that the method is used to inhibit at least one link in the process of fungus source formation, hyphae growth, conidia production, conidia germination and sexual reproduction of the wheat fusarium Fusarium graminearum , the pathogen of wheat fusarium graminearum.