CN1187618C - Reagent box for fast detecting methyl amphetamine and its preparation process - Google Patents

Abstract

The present invention relates to a kit for fast detecting methyl amphetamine by technologies of preventing methyl amphetamine monoclonal antibodies and technologies of immunity chromatography for colloidal gold. The kit is characterized in that a detection testing paper strip is orderly composed of sample filtrating paper, methyl amphetamine antibody glass fibers, a cellulose nitrate film and absorbent paper, wherein the methyl amphetamine antibody glass fibers are marked by colloidal gold; the T-line area (detection area) of the cellulose nitrate film is coated with methyl amphetamine-BSA, and the C-line area (quality control area) of the cellulose nitrate film is coated with goat anti mice antibodies; the prescription of the treatment solution of the sample filtrating paper comprises 0.05 to 3% of Brij, 0.01 to 2% of Triton x to 100, 0.5 to 3% of bovine serum albumin (BSA), 3 to 9% of sodium chloride (NaCl), 90 to 97% of purified water, and 0.01 to 0.03 mmol of trisodium phosphate (PB) buffer solutions. The present invention has the advantages of high sensitivity, strong specificity, favorable repetitiveness, high accuracy, simple operation process, no need for complicated devices, high detection speed and accurate detection.

Description

A kind of kit and preparation technology thereof who detects meth

One, technical field:

The present invention relates to utilize a kind of detection kit of anti-meth monoclonal antibody and colloidal gold immunochromatographimethod fabrication techniques, and the method for coming the meth in the human body urine with this kit.Belong to medical test articles for use field.

Two, background technology:

Meth (Methamphetamine claims crystal methamphetamine again, and deoxyephedrine is commonly called as ice) is a kind of medicine of sympathomimetic nerve excitation.Belonging to the central nervous excitation agent, also is the psychotropic substances of international and the strict control of China, is α and beta receptor excitant.Can cause the excitement of central nervous system to strengthen after acute higher concentration is taken, produce unusual floaty euphoria, susceptibility increases, heartbeat and accelerated breathing, and conscious energetic.Long-term or a large amount of users can produce tolerance and physical dependence, and cause abuse.Further toxic reaction can produce anxiety, bigoted vain hope, mental aberration and core function abnormality.The typical mental symptom that occurs during poisoning may not be easily distinguishable with schizophrenia.

Meth is discharged in body with forms such as amphetamine derivants usually, but still has excreting from urine with former medicine form about 40%.Therefore, occurring these relevant compounds in the urine shows and once used meth.

At the beginning of the eighties, international narcotics tide further spreads unchecked, and the China of " poison " evil of once thoroughly becoming extinct many decades also is subjected to the invasion and attack of drugs again.Show that according to relevant data the drug abuse person that China registers on the books has reached nearly 1,000,000 people at the bottom of calendar year 2001.And the upward actual drug abuse person of society also will be far away more than this numeral.In the last few years, because meth (be crystal methamphetamine, be commonly called as ice) raw material is synthetic more convenient, number every year of sucking meth was in quick increase.The eighties of last century later stage nineties particularly, a large amount of drug addicts from traditional smoke cannabis, heroin drugs such as (morphine classes) then suck that toxicity is more fierce, the efficacy of a drug longer meth action time.A large amount of dancing outreach that use also usually contained the meth composition in public places of entertainment such as the more metropolitan discotheques of China in recent years.Can make spirit excited during initial the abuse, amount of exercise obviously increases, and can bring out act of violence and criminal offense.Long-term abuse can cause insane, irritability, behavior out of control even the generation suicide and the behavior of hurting sb.'s feelings.

Serious day by day along with meth abuse, a kind of accurately, poison is looked in relevant department's prohibition of drug is very necessary to detection method easily and fast.The detection method of traditional meth has chromatography (as the GC/MS method) and immunization (Che Zongling, mass spectrometric determination methamphetamine, analytical test journal, 1997. (16): 45; Rasmussen S, et al.Methamphetamine in antomortem blood and urine by radioimmunoassay and GC/MS.J AnalToxi, 1989,13:263-267) chromatography is general accurate quantification, way of qualitative analysis, but apparatus expensive, operation length loaded down with trivial details, consuming time, is not suitable for handling sample in batches.Immunization mainly contains radioimmunology, euzymelinked immunosorbent assay (ELISA), fluorescent immune method etc., but similar defective is also arranged.Cost height, inconvenience, the time of detecting drugs with chromatography and immunization are long, particularly the first line public security officer are looked into that poison is banned taking addictive drugs and the monitoring of the prohibition of drug anti-drug institution of small city brings very big difficulty.

All have both at home and abroad relevant similar detection method bibliographical information (United States Patent (USP) 5,026,827. use N-(4-amine butyl) amphetamines and combination of proteins deposits yields can with tie the antibody that crystal methamphetamine closes; Kuroiwa, Forensic ScienceInternational 44:245-455,1990; Chinese patent 96120324.2, p-Methylamphetamine has specific monoclonal antibody, produce the hybridoma of this antibody, contain kit of this antibody and uses thereof), but because immune response, has determined that specificity, tire (specific activity) of the antibody produced are all variant in the factor of the aspects such as diversity of the diversity of the individual difference of immune animal, antigen site (antigen decision family) and immune response.The invention provides a kind of kit that detects meth from urine and preparation method thereof, make the present invention all have greatly improved at aspects such as stability and accuracies, kit of the present invention has tangible advance than existing correlation technique.

Three, summary of the invention:

The objective of the invention is to overcome existing method in technical defectives such as accuracy, practicality, utilize anti-basic amphetamine monoclonal antibody and a kind of detection kit of colloidal gold immunochromatographimethod fabrication techniques.

Principle of the present invention is:

Adopt immunity competition inhibition method to detect the existence of methyl amphetamine in the urine, promptly the meth (meth carrier) of protein labeling (bovine serum albumin(BSA), bovine thyroglobulin, ovalbumin) is with the limited antibody combining site of meth competition that exists in the urine.In kit, place a test strip, test strips is made up of four parts, is successively: the methyl amphetamine antibody glass fibre of filter sample paper, colloid gold label, T line zone (detection zone) bag by meth-BSA and at C line zone (Quality Control district) bag by the nitrocellulose filter of sheep anti-mouse antibody, thieving paper.After patient's urine sample is added drop-wise on the filter sample paper, urine will because of capillarity to thieving paper end chromatography, if there is not meth in the urine, mark the colloid gold particle of anti-methyl amphetamine antibody will move on the film behind the T line position in company with urine sample solution, by good methyl amphetamine carrier conjugates immune association reaction is taken place with T line bag, colloid gold particle makes in the accumulation of T line position and presents macroscopic pink lines, this negative result at the T line.If contain meth in the urine, they will compete limited antibody combining site with the meth-BSA of T line position bag quilt, when the meth concentration in patient's urine reaches a certain amount of, it will occupy all antibody combining sites of mark on the colloid gold particle, thereby stoped combining of meth carrier and its antibody colloidal gold on the T line position, like this, T line district does not have lines appearance, the ecbatic positive.

The present invention is achieved in that and comprises reagent strip and external packing box, reagent strip begins successively to be made up of filter sample paper, the collaurum scraps of paper, the nitrocellulose filter, the thieving paper that contain detection zone and Quality Control district from the application of sample end, containing the formulation by weight ratio in the described filter sample paper Treatment Solution is: surfactant upright logical X-100 0.01～2% in the wrong, bovine serum albumin(BSA) 0.5～3%, sodium chloride 3～9%, pH value are sodium phosphate buffer concentration 0.01～0.03mmol/l of 7.2, it is characterized in that: also contain detergent Bu Lijie 0.05～3% in the prescription, all the other are pure water.A kind of preparation technology who detects the meth kit, comprise reagent strip and external packing box, reagent strip begins successively to be made up of filter sample paper, the collaurum scraps of paper, the nitrocellulose filter, the thieving paper that contain detection zone and Quality Control district from the application of sample end, it is characterized in that: described filter sample paper carries out dip treating with filter sample paper Treatment Solution earlier, then reagent strip is adopted vacuum drying process, temperature is at 15 ℃～25 ℃; 2～5 hours vacuum drying time, the atmospheric pressure when dry in the vacuum tank should be less than 0.1mba.

The present invention only needs 5-8 minute to the detection of meth, and detection sensitivity can reach 800ng/ml.The present invention is being provided with extremely important to thresholding (CUTOFF value), the too high also test positive of the normal rational use of medicines that causes easily of sensitivity, too omission then can be caused in the end in sensitivity, and the present invention by monoclonal antibody invention and the innovation of technology, invented preparation method of the kit of the sensitivity (thresholding) of suitable clinical needs.Through the commonly used drug abuse of kind more than 40 and the cross reaction experimental study of similar medicine of molecular structure and common drug commonly used are shown that the present invention is to the equal no cross reaction of these materials, high specificity.

The performance index situation of product of the present invention is:

1) highly sensitive

The present invention detects the existence of methyl amphetamine in the urine, and detecting threshold value is 800ng/ml.The present invention and GC/MS experiment are compared discovery:

A. detect 120 routine healthy volunteers urine samples, both all negative (100% meets).

B.55 part is taken from the urine sample of clinical labororatory, confirms through GC/MS, and the concentration of methyl amphetamine is from 1250 ~ 100 in its urine, and 000ng/ml does not wait, and the present invention detects and all gives positive findings.

2) good reproducibility

The present invention is through detecting three different regions, and wherein 60 duplicate samples are 500ng/ml, all detects negative; 60 duplicate samples methyl amphetamine concentration are 2, and 000ng/ml all detects positive.

3) degree of accuracy height

It is as follows to carry out the precision detection with the standard items of preparing, and 500ng/ml all gives negative findings; 1500ng/ml all gives positive findings.

Methyl amphetamine concentration	Amount detection	Positive	Negative	Critical	Coincidence rate
						500ng/ml	40	0	0	0	100％
700ng/ml	40	0	39	1	97.5％
						1000ng/ml	40	39	0	1	97.5％
1500ng/ml	40	40	0	0	100％

4) high specificity

Detection kit of the present invention is tested different medicines, drug metabolite and other compounds that may occur in urine, and the result is as follows:

A. the compound of following similar is equal negative findings less than following concentration the time.

Compound concentration (ng/ml)
	D-amphetamine D-Amphetamine 50,000
Chloroquine Chloroguine 50,000
	+/-ephedrine element (+/-)-Ephedrine 50,000
(+)-meth, (+)-dechlorination ephedrine (+)-Methamphetamine 1,000
	(-)-Methamphetamine ((-)-meth, (-)-dechlorination ephedrine) 25,000
(+/-)-3,4-methylene-dioxy amphetamine 2,000 (+/-)-3,4-methylenedioxyamphetamine
	Procaine Procaine 10,000
β-phenyl ethylamine β-Phenylethylamine 50,000
	Ranitidine Ranitidine 50,000

B. the compound of following similar may produce positive findings more than or equal to following concentration the time.The result was negative when following compounds was equal to or less than 100 μ g/ml in concentration.

Compound concentration (μ g/ml)
	Paracetamol Acetami nophen 100
Hydrocodone, dihydrocodeinone Hydrocodone 100
	Albumin, albumin Albumin 100
Amitriptyline Amitriptyline 100
	L-amphetamine L-amphetamine 100
Ampicillin Ampicillin 100
	Aspartame, aspartame Aspartame 100
Aspartoyl, phenyalanine methyl ester Asparton 100
	Aspirin Aspirin 100
Atropine Atropine 100
	Benzocaine Benzocaine 100
Cholerythrin Bilirubin 100
	Benzoyl ecgonine, benzoyl ecgonine Bengoylecgonine 100
Brompheniramine, brompheniramine (+) Brompheniramine 100

Caffeine Caffeine 100
	Chlortrimeton, chlorpheniramine Chlorphenamine maleate 100
Chlorphenamine, chlorpheniramine Chlorpheniramine 100
	Creatine, creatine Creatine 100
Dexbrompheniramine Dexbrompheniramine 100
	(dextrorotation) dextromethorphan Dextromethorphan 100
Diazepam, stable Diazepam 100
	4-dimethylamidoantipyrine (phenopyrine) 100 4-Dimethylaminoantipyrine
Dopamine D opamine 100
	Doxylamine Doxylamine 100
Serratd edge alkali Ecgonine 100
	Ecgonine methyl Ecgonine Methylester 100
The plain Ephedrine 100 of Chinese ephedra (alkali)
	Adrenaline Epinephrine 100
Erythromycin E rythromycin 100
	Ethanol Ethanol 100
Glucose Glucose 100
	Guaiacol glycerol ether Guaiacol Glyceryl Ether 100
Hb H emoglobin 100
	Acetone Acetone 100
Imipramine Imipramine 100
	Isoprel Isoproterenol 100
Lidocaine Lidocaine 100
	Oxycodone; 14-hydroxy dioxetane codeinone Oxycodone 100
Methylphenidate, ritalin Methylphenidate 100
	N-methyl ephedrine N-Methyl-Ephedrine 100
Pethidine, Sauteralgyl Meperidine 100
	Phenylephrine L-Phenylephrine 100
Norephedrine, norephedrine Norephedrine 100

Naproxen Naproxen 100
	Morphine Morphine 100
Oxalic acid Oxalic acid 100
	Oxazepam, Sebril Oxazepam 100
Methadone, methadone Methadone 100
	Penicillin-G Penicillin-G 100
Vitamin B2 Riboflavin 100
	Phenothiazine Phenothiazine 100
(+/-)-3,4 methylene-dioxy amphetamines 100 (+/-)-3,4-melhylenedioxyamphetamine
	Phenyl ethylamine-Phenylepylamine 100
Procaine Procaine 100
	Quinoline Buddhist nun fourth Quinidine 100
(benzene Lamine) pheniramine, pheniramine Pheniramine 100
	The big Sulindac 100 of Su Ling
Tyrasamine Tyramine 100
	Vitamin C Vitamin C 100

One aspect of the present invention provides a kind of preparation method of anti-meth cell strain of monoclonal antibody, and the preparation of anti-meth monoclonal antibody purifying, and content comprises:

1) preparation of meth-BSA (bovine serum albumin(BSA)) and the crosslinked body of meth-BTG (bovine thyroglobulin);

After in external antigen enters body, the human immune system will produce immune response to the antigen that enters such as bacterium, virus, dissident's large protein etc., and produces specific antibody, and is not infected with the protection body.When antigen molecule is very little, or even during compound, then itself can not produce antibody by stimulating immune system, also just can not form antigen-antibody reaction.The drugs that we need detect are exactly multi-form compound.Set up the immunological detection method of drugs, at first will obtain specific drugs antibody, and can set up the reactive system of Ag-Ab.Because drugs itself are the very little compounds of molecular weight, itself can not produce antibody by stimulating immune system, therefore, drugs and larger protein molecule need be linked up, form the protein-crosslinking body, keep the characteristic of drugs structure simultaneously, can form complete antigenic structure like this, finish immune response.

The most frequently used cross-linking agent is a macro-molecular protein.Comprising bovine serum albumin(BSA) (BSA), bovine thyroglobulin (BTG), ovalbumin (OB) etc.We use the crosslinked body of BSA as immunogene, and the preparation monoclonal antibody is carried out the monoclonal antibody screening with BTG simultaneously, and the assembling reagent strip.

A. main material:

Meth M-AMP;

BSA and BTG;

MES；

NaCl；

Na ₂HPO ₄And NaH ₂PO ₄

EDC；

More than be the commercially available prod.

B. method:

1. dispose the MES-NaCl damping fluid, BSA or BTG are dissolved in this damping fluid, concentration is 10-20mg/ml.

2. use above-mentioned buffer solution: preparation 5-8mg/ml meth solution.

3. use the crosslinked BSA of EDC or BTG and meth,, remove free meth with the PB dialysis.

2) with purifying meth-BSA immune balb/c mice;

3) splenocyte and the murine myeloma cell with immune mouse merges, and carries out the monoclonal antibody screening with the meth-BTG behind the purifying, by obtaining hybridoma after the cell cloneization;

4) with the method for mouse ascites culturing in vivo hybridoma, purifying prepares anti-meth monoclonal antibody.

Another aspect of the present invention provides the collaurum single stage method of utilizing above-mentioned anti-meth monoclonal antibody to detect the kit and the method for meth.

Another aspect of the present invention provides a kind of production technology of new collaurum single stage method detection kit.

Production technology has very big influence to the stability of kit, behind the production technology nitrocellulose filter envelope antigen of bibliographical information or the antibody and the colloid gold label all-glass paper all use constant temperature and humidity drying, the vacuum drying of this process using, 1) temperature is at 15-25 ℃; 2) the 2-5 hour vacuum drying time; Atmospheric pressure when 3) dry in the vacuum tank should be less than 0.1 millibar (mba).This technology has been guaranteed the homogeneity of kit and better stable, and kit can be stablized preservation more than 2 years at normal temperatures.

Also aspect of the present invention, in order to improve the performance characteristics of kit, the progressive of guarantee reagent box, the present invention also innovates on prescription.

The kit preparation: preparation the present invention detects the kit of the existence of methyl amphetamine in the urine, and main antibody antigen material is the BSA cross-linking agent (MAMP-BSA) of homemade basic amphetamine monoclonal antibody of the inventor and meth.The process of preparation is as follows:

1. the preparation of collaurum: prepare collaurum with trisodium citrate reduction method.

2. immune colloid gold preparation: the PH that regulates colloidal gold solution with sal tartari or the 0.1M hydrochloric acid of 0.2M is to set point value, with the anti-M-AMP monoclonal antibody and the colloidal gold solution rapid mixing mark of the suitable concn of an amount of volume.By centrifugal purification immuno-gold labeling thing.

3. meth (M-AMP) detection kit preparation

1) main material

1. meth-BSA compound (M-AMP-BSA)

2. anti-M-AMP monoclonal antibody

3. sheep anti-mouse igg antibody

4. nitrocellulose membrane

5. immune colloid gold solution

6. plastic casing (forming) by upper and lower cover

7. thieving paper

8. offset plate

2) preparation

With anti-M-AMP monoclonal antibody configuration T line solution, sheep anti-mouse igg antibody configuration C line solution, then they are put T line, C line zone on the nitrocellulose filter respectively; The immune colloid gold solution of deployed debita spissitudo is put on the glass fibre; Paste with thieving paper combination again and become test strips on the offset plate, test strips is assembled in the plastic casing, put in the vacuum pump that sealing is kit after the vacuum drying.

1. the composition of kit:

The present invention mainly is made up of the two large divisions: reagent strip and external packing box

The detectable bar is the core of whole invention, begins from the application of sample end to be successively: filter sample paper, the collaurum scraps of paper, T line zone (detection zone) bag by meth-BSA and at C line zone (Quality Control district) bag by the nitrocellulose filter of sheep anti-mouse antibody, four parts of thieving paper.These four parts all paste on the offset plate of an adhesive sticker successively, cut into wide 4.5mm then, and the strip of long 80mm is in the plastic casing that designs of packing into.The feature of this assembly is: filter sample paper has used special closed reagent prescription of the present invention; Wrap respectively by meth-BSA compound (M-AMP-BSA), sheep anti-mouse igg antibody in T on the nitrocellulose filter, C line district.

Operating process of the present invention is simple, need not complex apparatus, and detection speed is very fast, accuracy is high.The result judges very clear.

2. filter sample paper Treatment Solution recipe ratio:

The nitrocellulose filter self character is hydrophobic, and it is because the cause that matter moisture exists between in the film that moisture why can chromatography enters nitrocellulose filter.Film is hydrophobic extremely important, is hydrophobic such as detected material, and the non-thing opposite sex effect of it and film causes the tangible non-specific mark of film; Losing of matter moisture can cause long term storage and difficulty between in the film; Heavy moistening speed and chromatography speed may be too low and influence the effect of express-analysis.Therefore the hydrophobicity of film is that the kit preparation needs one of gordian technique difficult point that solves, in order to reduce or eliminate these problems, the present invention selects to use closed reagent to handle filter sample paper and solves, and it can guarantee the stability and the repeatable characteristics that allow system guarantee product of helping an of the best when experiment.It can reduce the level of non-specific background spot significantly.And when carrying out analytical test, can make film keep the heavy wet characteristic of average homogeneity with sample.But the hydrophobic non-specific adsorption and the specific reaction of antigen-antibody repel often mutually.For example: if closed reagent reduces nonspecific interaction, it also will reduce the amount of specificity combination usually.The present invention uses following prescription just well to solve this technological difficulties, compares with existing technical method, has obviously overcome because nonspecific reaction causes the phenomenon of false positive results, and therefore, the present invention has obviously improved the accuracy that detects.

The recipe ratio of filter sample paper Treatment Solution

No.	Title	Content
			No.1	Brij	0.05-3％
No.2	Triton X-100	0.01-2％
			No.3	Bovine serum albumin(BSA) (BSA)	0.5-3％
No.4	Sodium chloride nacl	3-9％
			No.5	Pure water	90-97％
No.6	Sodium phosphate (PB) damping fluid	0.01-0.03mmol/l

3. the drying process of detectable bar

Production technology has very big influence to the stability of kit, the stability of kit depends primarily on the stability of antigen-antibody, and key factor just is the degree of drying and the dry homogeneity of nitrocellulose filter and colloid gold label all-glass paper, behind the production technology nitrocellulose filter envelope antigen of bibliographical information or the antibody and the colloid gold label all-glass paper all use constant temperature and humidity drying, the vacuum drying process feature of this process using:

I. temperature is at 15-25 ℃;

Ii. 2-5 hour vacuum drying time;

Atmospheric pressure when iii. dry in the vacuum tank should be less than 0.1 millibar (mba).

This technology has been guaranteed the homogeneity of kit and better stability, and the normal temperature term of validity of the similar inventions of foreign literature report all at 1 year below half, is preserved more than 2 years and the present invention is stable at normal temperatures.

Four, embodiment:

Embodiment 1:

From urine, detect the kit and the making thereof of meth:

1, the making of detectable bar

2, the prescription of the filter sample paper Treatment Solution of Shi Yonging

No.	Title	Content
			No.1	Brij	0.05％
No.2	Triton X-100	0.01％
			No.3	Bovine serum albumin(BSA) (BSA)	0.5％
No.4	Sodium chloride nacl	3％
			No.5	Pure water	90％
No.6	Sodium phosphate (PB) damping fluid	0.01mmol/l

3, the interpolation of the making of reagent strip and all ingredients

4, the drying process of detectable bar

A) temperature is at 15 ℃;

B) the 5 hours vacuum drying time;

0.05 millibar in atmospheric pressure (mba) when c) dry in the vacuum tank.

5, the encapsulation of detection kit and use thereof

The use of plastic casing of the present invention makes that the operating process that detects is convenient:

I. get the about 0.2ml of urine sample with suction pipe, all splash into well then.

Ii. after dripping sample, read the result in 5 minutes.

Iii. two colour bands appear in watch window, and there is negative result in No Poison in the expression sample; Watch window only colour band occurs in C line district, and there is positive result in Poison in the expression sample.

Embodiment 2:

From urine, detect the kit and the making thereof of meth:

1, the making of detectable bar

2, the prescription of the filter sample paper Treatment Solution of Shi Yonging

No.	Title	Content
			No.1	Brij	3％
No.2	Triton X-100	2％
			No.3	Bovine serum albumin(BSA) (BSA)	3％
No.4	Sodium chloride nacl	9％
			No.5	Pure water	97％
No.6	Sodium phosphate (PB) damping fluid	0.01mmol/l

3, the interpolation of the making of reagent strip and all ingredients

4, the drying process of test agent bar

A) temperature is at 25 ℃;

B) the 2 hours vacuum drying time;

0.01 millibar in atmospheric pressure (mba) when c) dry in the vacuum tank.

5, the encapsulation of test agent box and use thereof

The use of plastic casing of the present invention makes that the operating process that detects is convenient:

I. get the about 0.2ml of urine sample with suction pipe, all splash into well then.

Ii. after dripping sample, read the result in 5 minutes.

Iii. two colour bands appear in watch window, and there is negative result in No Poison in the expression sample; Watch window only colour band occurs in C line district, and there is positive result in Poison in the expression sample.

Claims (4)

1, a kind of detection meth kit, comprise reagent strip and external packing box, reagent strip begins successively to be made up of filter sample paper, the collaurum scraps of paper, the nitrocellulose filter, the thieving paper that contain detection zone and Quality Control district from the application of sample end, containing the formulation by weight ratio in the described filter sample paper Treatment Solution is: upright logical X-100 0.01～2% in the wrong, bovine serum albumin(BSA) 0.5～3%, sodium chloride 3～9%, pH value are sodium phosphate buffer concentration 0.01～0.03mmol/l of 7.2, it is characterized in that: also contain Bu Lijie 0.05～3% in the prescription.

2, require 1 described detection meth kit according to profit, it is characterized in that: all the other are pure water in the recipe ratio of filter sample paper Treatment Solution.

3, a kind of preparation technology who detects the meth kit by claim 1 or 2, comprise reagent strip and external packing box, reagent strip begins successively to be made up of filter sample paper, the collaurum scraps of paper, the nitrocellulose filter, the thieving paper that contain detection zone and Quality Control district from the application of sample end, it is characterized in that: described filter sample paper carries out dip treating with filter sample paper Treatment Solution earlier, then reagent strip is adopted vacuum drying process, temperature is at 15 ℃～25 ℃; 2～5 hours vacuum drying time, the atmospheric pressure when dry in the vacuum tank should be less than 0.1mba.

4, the preparation technology of detection meth kit according to claim 3, it is characterized in that: described filter sample paper Treatment Solution formulation by weight ratio is: upright logical X-100 0.01～2% in the wrong, bovine serum albumin(BSA) 0.5～3%, sodium chloride 3～9%, Bu Lijie 0.05～3%, pH value are sodium phosphate buffer concentration 0.01～0.03mmol/l of 7.2, and all the other are pure water.