CN118750413B

CN118750413B - Fermented oil with repairing, moisturizing and antioxidant effects, preparation method and application thereof, and daily chemical product

Info

Publication number: CN118750413B
Application number: CN202411240017.4A
Authority: CN
Inventors: 谢坤; 刘浩
Original assignee: Baitide Pharmaceutical Biotechnology Guangdong Co ltd
Current assignee: Guangdong Youxue Pharmaceutical Technology Co ltd
Priority date: 2024-09-05
Filing date: 2024-09-05
Publication date: 2024-11-22
Anticipated expiration: 2044-09-05
Also published as: CN118750413A

Abstract

本申请属于化妆品生产技术领域，公开了一种具有修护保湿抗氧化功效的发酵油，具有修护保湿抗氧化功效的发酵油通过含有活菌的发酵液对复合油发酵得到；含有活菌的发酵液为含有毕赤酵母活菌的发酵液；含有毕赤酵母活菌的发酵液与复合油的质量比为1：1～3；复合油按质量分数计，包括20～50%的向日葵籽油，1～3%的水飞蓟籽油，20～50%的油橄榄果油，5～10%的乳木果油，10～20%的稻糠油；通过含有毕赤酵母对复合油进行发酵，使发酵油中具有自由基清除能力以及促进细胞迁移能力的活性物质的含量得到进一步的提升，此外，本申请还公开了上述具有修护保湿抗氧化功效的发酵油的制备方法、用途和一种化妆品。The present application belongs to the technical field of cosmetic production, and discloses a fermented oil with repairing, moisturizing and antioxidant effects. The fermented oil with repairing, moisturizing and antioxidant effects is obtained by fermenting a composite oil with a fermentation broth containing live bacteria; the fermentation broth containing live bacteria is a fermentation broth containing live Pichia pastoris; the mass ratio of the fermentation broth containing live Pichia pastoris to the composite oil is 1:1-3; the composite oil comprises, by mass fraction, 20-50% of sunflower seed oil, 1-3% of milk thistle seed oil, 20-50% of olive oil, 5-10% of shea butter, and 10-20% of rice bran oil; by fermenting the composite oil with Pichia pastoris, the content of active substances with free radical scavenging ability and cell migration promoting ability in the fermented oil is further improved. In addition, the present application also discloses a preparation method, use and a cosmetic of the above-mentioned fermented oil with repairing, moisturizing and antioxidant effects.

Description

Fermented oil with repairing, moisturizing and antioxidant effects, preparation method and application thereof, and daily chemical product

Technical Field

The invention relates to the technical field of cosmetic production, in particular to a fermented oil with repairing, moisturizing and antioxidation effects, a preparation method and application thereof, and a cosmetic.

Background

Cell migration is closely related to skin repair, particularly during wound healing and tissue regeneration. This process typically involves the following key stages: stage of inflammation: in the early stages of skin damage, immune cells (such as neutrophils and macrophages) migrate first to the damaged site. These cells help to clear bacteria and tissue debris while releasing various cytokines and growth factors, facilitating the subsequent repair process; fibroblast migration and proliferation phase: as the inflammatory response subsides, fibroblasts and other cell types (e.g., fibroblasts) begin migrating toward the damaged area. These cells not only proliferate, but also synthesize and secrete collagen and other extracellular matrix components to repair damaged tissue structures; stage of neovascularization: to support the growth of new tissue, endothelial cells migrate and form new blood vessels (angiogenesis). This process ensures that the damaged area is able to receive sufficient oxygen and nutrition to support cell proliferation and tissue regeneration; epithelial cell migration and re-epithelialization phase: epidermal cells (keratinocytes) migrate from the edge of the wound toward the center, covering the damaged area, forming a new layer of skin. This process is particularly important for restoring skin barrier function; remodeling stage: after the initial repair of the tissue is completed, the cells and matrix of the damaged site are gradually remodelled to enhance the strength and function of the new tissue. This stage may last from weeks to months.

In these processes, cell migration is indispensable. For example, intercellular communication can regulate migration, proliferation and differentiation of cells through the action of various signaling molecules (e.g., cytokines, chemokines and growth factors), ensuring that each type of cell functions at the correct time and place to achieve efficient tissue repair, abnormalities in cell migration regulation, and the like, may lead to poor healing or chronic wounds such as diabetic ulcers or decubitus ulcers, and the like. Therefore, the deep understanding of the mechanism and regulation means of cell migration is of great importance for developing new therapies that promote wound healing.

The antioxidant capacity of skin refers to the capacity of the skin itself and antioxidant substances obtained by external skin care products to resist oxidative stress caused by free radicals. Free radicals are one of the main causes of skin aging, pigmentation, inflammation and other skin problems. The antioxidant defense system of skin comprises endogenous antioxidant enzymes (such as superoxide dismutase and catalase) and exogenous antioxidant substances (such as vitamin C, vitamin E and polyphenol compounds), and DPPH free radical scavenging rate experiment is a simple and effective method for evaluating the antioxidant capacity of substances. Substances with high removal rate can generally effectively neutralize free radicals, and are beneficial to maintaining skin health and improving the antioxidant capacity of the skin. The method provides important scientific basis for the research and development of the antioxidant skin care product, and indirectly helps to improve the skin health state.

Chinese patent application 202311730896.4 discloses a preparation of natural plant fermented oil and its application in cosmetics, the preparation of natural plant fermented oil includes the following steps: a. sieving Oryza Glutinosa powder, adding soybean oil and sterile water, stirring to paste, inoculating yeast, fermenting, and subculturing to obtain distiller's yeast; b. grinding distiller's yeast, adding sterile water, stirring, and extracting to obtain yeast fermentation broth; c. using vegetable oil as an oil phase, using a fermentation culture medium as a water phase, and emulsifying by using a micro-jet homogenizer to form an emulsion; d. inoculating yeast fermentation broth into the emulsion for fermentation, then sterilizing, adding hydroxyethyl cellulose for dewatering, and filtering to obtain fermented vegetable oil;

The natural plant fermentation oil prepared by the scheme adopts saccharomycetes in natural fermentation distiller's yeast, contains lipase with stronger grease decomposition capability, and can decompose macromolecular groups in grease into micromolecular groups to the greatest extent. The plant fermentation oil has various effects of antioxidation, crease resistance, moisture preservation and the like; meanwhile, the scheme adopts a micro-jet homogenizing method to homogenously emulsify an oil phase and a water phase into oil-in-water type tiny emulsified particles, so that the reaction area between bacterial liquid and vegetable oil is enlarged, the fermentation time is shortened, and the fermentation efficiency is improved; however, as can be seen from section 21 of the description of the present embodiment, the vegetable oil selected for the present embodiment is at least one of rhodiola rosea oil, arnica oil and bilberry seed oil.

The Chinese patent application 202211414904.X discloses a preparation method and application of a long-acting low-irritation multipurpose anti-wrinkle supermolecule composition, which comprises raw and auxiliary materials of grease, active ingredients, degradable high polymer materials, phospholipids, solid lipids, emulsifying agents, antioxidants, preservatives, moisturizing and soothing agents, PH regulators and deionized water, has long-acting slow release function, further remarkably reduces irritation of anti-wrinkle active substances, can remarkably improve permeation efficiency of multiple anti-wrinkle active substances, realizes targeted delivery of dermis layers, enriches anti-aging active substances in the dermis layers after penetrating through the stratum corneum layers, permanently exerts anti-wrinkle effects, realizes external care and internal skin nourishing and refreshing effects, and is accurate and efficient in anti-wrinkle; meanwhile, the macromolecules of the cream can be thinned into micro molecules, so that the fluidity is enhanced, and one bottle of cream can be used as essence water cream and the like; the product can also be used as a raw material to be applied to a formula product; has the characteristics of multiple functions of one bottle;

Further observing paragraphs 12-13 of the specification, wherein the active ingredients are retinol and derivatives, lactose yeast, bifenthrin, ergothioneine, vitronectin, SOD, VE and derivatives, plant flavonoids (radix Puerariae or semen Ginkgo), silymarin, tea polyphenols, fructus Gardeniae extract, kava root extract, radix Notoginseng extract, mi Luo Mu extract, grape seed extract, etc. anti-wrinkle plant extracts, ferulic acid, hydrolyzed collagen, ubiquinone, carnosine, resveratrol, ferulic acid, ceramide, glutathione, palmitoyl polypeptide, acetyl polypeptide, nutmeg polypeptide, and/or peptide-like antiwrinkle peptides;

the grease is any one of glabrous greenbrier rhizome oil, ginseng seed oil, octyldodecanol, polydimethylsiloxane, squalane, caprylic/capric triglyceride, isononyl isononanoate, isopropyl myristate, shea butter, white pond flower seed oil, olive oil, camellia oil, jojoba seed oil, grape seed oil, white pond flower seed oil, dioctyl carbonate, hydrogenated polyisobutene, camellia seed oil and/or GTCC and a combination thereof;

However, further observations were made regarding the preparation of the design compositions of paragraphs 72 to 75 of this protocol: "(1) oil phase preparation: dissolving 0.2 part of hydroxy pinacolone retinoic acid ester, 0.05 part of palmitoyl tripeptide-5, 0.05 part of palmitoyl pentapeptide-4, 0.5 part of 4-tertiary butyl cyclohexanol, 1 part of phospholipid and a proper amount of solid lipid emulsifier in 10 parts of grease containing white pond flower seed oil, squalane, GTCC and the like, and fully stirring under the condition of inert gas atmosphere and set temperature to ensure that the dissolution and dispersion are uniform;

(2) Preparing an aqueous phase: weighing 0.10 part of acetyl hexapeptide-8, a certain amount of gardenia extract, kava root extract, pseudo-ginseng root extract, pachyman Luo Mu extract and other plant extracts, a proper amount of preservative, stabilizer, glycerol and the like, and adding the plant extracts into the rest water to serve as water phase for standby;

(3) Maintaining inert gas atmosphere and temperature, slowly adding the oil phase in the step (1) into the water phase under the shearing condition, and performing ultrasonic treatment by a probe, high-pressure homogenization or microjet treatment to obtain the long-acting low-stimulation multipurpose anti-wrinkle supermolecule composition;

(4) Texture transition test: the prepared anti-wrinkle supermolecule composition is subjected to a smearing test by taking the same volume, and the effect of converting the cream from a liquid state into the cream texture in the smearing process is recorded;

it can be seen that this protocol does not ferment the composition.

The problem that this scheme needs to solve: how to develop a fermented oil with the functions of repairing, preserving moisture and resisting oxidation.

Disclosure of Invention

The application aims to provide a fermentation oil with repairing, moisturizing and antioxidant capabilities, which is prepared by fermenting compound oil prepared from sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice chaff oil through fermentation liquor containing pichia pastoris active bacteria, so that the content of active substances with free radical scavenging capability and cell migration promoting capability in the fermentation oil is improved;

the application also aims to provide a preparation method of the fermented oil with the effects of repairing, preserving moisture and resisting oxidation;

In addition, the application also discloses application of the fermented oil with the repairing, moisturizing and antioxidant effects and a cosmetic.

In order to achieve the above purpose, the application discloses a fermentation oil with repairing, moisturizing and antioxidation effects, which is obtained by fermenting compound oil by fermentation liquor containing living bacteria;

The fermentation liquor containing the viable bacteria is fermentation liquor containing pichia pastoris viable bacteria;

the mass ratio of the fermentation liquor containing the pichia pastoris viable bacteria to the compound oil is 1:1 to 3;

the compound oil comprises the following components in percentage by mass:

20-50% of sunflower seed oil;

1-3% of silybum marianum seed oil;

20-50% of olive fruit oil;

5-10% of shea butter;

10 to 20 percent of rice bran oil.

Vitamin E and other antioxidant components in sunflower seed oil can neutralize free radicals and reduce the damage of oxidative stress to skin, thereby delaying skin aging and reducing the formation of fine lines and wrinkles; meanwhile, fatty acid and vitamin E in the sunflower seed oil can promote regeneration and repair of skin cells, and is beneficial to improving the overall health of skin;

The silymarin seed oil contains high-concentration silymarin, which is a powerful antioxidant, can resist the invasion of free radicals, reduce the damage of oxidative stress to skin cells, and simultaneously, the silymarin seed oil can delay the skin aging process by neutralizing the free radicals, reduce the formation of fine lines and wrinkles, and improve the skin elasticity and compactness;

the olive oil contains abundant vitamin E, is a powerful antioxidant, can neutralize free radicals, reduces the damage of oxidative stress to skin, can delay skin aging, reduce the generation of fine lines and wrinkles, and maintains the elasticity and compactness of the skin through the antioxidation;

The shea butter contains natural plant polyphenol, has antioxidation property, can reduce the damage of oxidative stress to skin, delay skin aging, and can slow down the skin aging process, reduce the formation of fine lines and wrinkles and promote the elasticity and compactness of skin through antioxidation;

Rice bran oil is a natural vegetable oil extracted from the husk and germ of rice, which is rich in squalene and oryzanol, and has strong antioxidant effect, and can reduce the damage of free radical to skin.

Preferably, the compound oil comprises the following components in percentage by mass:

30-40% of sunflower seed oil;

1-3% of silybum marianum seed oil;

30-40% of olive fruit oil;

5-10% of shea butter;

12-18% of rice bran oil.

Preferably, the fermentation broth containing the pichia pastoris is prepared by fermenting grains by pichia pastoris;

The cereal is at least one selected from oat, corn, buckwheat and soybean;

the mass ratio of the pichia pastoris to the cereal is 1:3 to 8.

Preferably, the grains are a mixture of oat, corn and buckwheat, and the mass ratio of oat, corn and buckwheat in the mixture of oat, corn and buckwheat is 1: 2-3: 0.8 to 1.5.

In addition, the application also discloses a preparation method for preparing the fermented oil with the repairing, moisturizing and antioxidant effects, which comprises the following steps of:

Step 1: mixing sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice chaff oil according to a mass ratio to obtain compound oil;

step 2: mixing the fermentation liquor containing living bacteria with the compound oil prepared in the step1, fermenting, centrifuging and collecting an oil phase to obtain the fermentation oil with the effects of repairing, preserving moisture and resisting oxidation.

Further preferably, the step 2 further comprises the substeps of:

step A1: drying and crushing grains to obtain powder, and then mixing the powder with peptone, yeast extract, disodium hydrogen phosphate and pure water to obtain an activated culture medium;

Step A2: inoculating pichia pastoris into an activation culture medium for culture to obtain fermentation liquor containing living bacteria;

Step A3: mixing the fermentation liquor containing living bacteria with the compound oil prepared in the step 1, and fermenting to obtain the fermentation oil with the effects of repairing, preserving moisture and resisting oxidation.

In addition, the application also discloses application of the fermented oil with the repairing, moisturizing and antioxidant effects in cosmetics.

In addition, the application also discloses a cosmetic which contains 1-20wt% of the fermented oil with the repairing, moisturizing and antioxidation effects.

Preferably, the cosmetic is in the form of a patch, a paste, a liquid, a spray, a gel, an emulsion or a cream.

The beneficial effects of the application are as follows:

Vitamin E and other antioxidant components in sunflower seed oil which are one of important raw materials of the fermentation oil with the effects of repairing, preserving moisture and resisting oxidation can neutralize free radicals, reduce the damage of oxidative stress to skin, delay skin aging and reduce the formation of fine lines and wrinkles; meanwhile, fatty acid and vitamin E in the sunflower seed oil can promote regeneration and repair of skin cells, and is beneficial to improving the overall health of skin;

In addition, the compound oil prepared from sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice chaff oil is fermented through the fermentation liquid containing pichia pastoris active bacteria, so that the content of active substances with free radical scavenging capability and cell migration promoting capability in the fermentation oil is further improved.

Detailed Description

The present invention will be described more fully hereinafter with reference to the accompanying drawings, in which specific conditions, either conventional or manufacturer-suggested, are not explicitly shown. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

Before the examples are shown, the raw materials involved in each example and the method for preparing the fermented oil in each example are described as follows except for the specific description:

the raw materials involved in the examples are shown in table 1:

TABLE 1

Raw materials	Suppliers (suppliers)	Raw materials	Suppliers (suppliers)
				Sunflower seed oil	Jiangxi Yisen Source plant fragrances Co.Ltd	Shea butter	Shanxi Living Biotech Co., ltd
Silybum marianum seed oil	Shanxi Laisent Biotechnology Co.Ltd	Rice bran oil	Wuhan Lanabai pharmaceutical chemical Co., ltd
				Olive fruit oil	Jiangxi Yisen Source plant fragrances Co.Ltd	Pichia pastoris	Shanghai Xuan Ke Biotechnology Co.Ltd
Peptone	Jinan Qingyuan New Material Co.Ltd	Yeast extract	Siam Ice cereal Biotechnology Limited liability company

Examples 1 to 5

A fermented oil with repairing, moisturizing and antioxidation effects is prepared by the following steps:

Step 2:

step A2: inoculating pichia pastoris into an activation culture medium, and culturing for 24 hours at a constant temperature of 28 ℃ to obtain a fermentation broth containing living bacteria, wherein the mass ratio of the pichia pastoris to the grains is 1:5, a step of;

step A3: mixing the fermentation liquor containing living bacteria with the compound oil prepared in the step 1 according to the following ratio of 1:2, mixing and fermenting at the constant temperature of 28 ℃ for 24 hours, and then centrifuging and collecting an oil phase to obtain the fermented oil with the effects of repairing, preserving moisture and resisting oxidation.

Wherein the grains are specifically oat, the mass fraction of powder in an activation culture medium is 29%, the mass fraction of protein is 2%, the mass fraction of yeast extract is 2%, the mass fraction of disodium hydrogen phosphate is 1%, and the balance is pure water;

the formulations of the compound oils in examples 1-5 are shown in Table 2:

TABLE 2

Example 6

Substantially the same as in example 1, except that the cereal is a mixture of oat, corn and buckwheat, and the mass ratio of oat, corn and buckwheat is 1:2.5:1.2.

Example 7

Substantially the same as in example 1, except that the cereal is a mixture of corn and buckwheat, and the mass ratio of corn and buckwheat is 2.5:1.2.

Example 8

Substantially the same as in example 1, except that the mass ratio of pichia pastoris to cereal is 1:3.

Example 9

Substantially the same as in example 1, except that the mass ratio of pichia pastoris to cereal is 1:8.

Example 10

Substantially the same as in example 1, except that the mass ratio of the fermentation broth containing viable bacteria to the complex oil was 1:1.

Example 11

Substantially the same as in example 1, except that the mass ratio of the fermentation broth containing viable bacteria to the complex oil was 1:3.

Comparative examples 1 to 5

Substantially the same as in example 1, except that the formulation of the compound oil is as shown in Table 3:

TABLE 3 Table 3

In the above comparative examples 1 to 5, each component was added in parts by mass, but the total mass of the compound oil of each comparative example was the same as that of the compound oil of example 1.

Comparative examples 6 to 10

Substantially the same as in example 1, except that the formulation of the compound oil is as shown in Table 4:

TABLE 4 Table 4

Comparative example 11

Substantially the same as in example 1, except that galactose yeast was used instead of pichia pastoris.

Comparative example 12

Substantially the same as in example 1, except that sake yeast was used instead of pichia pastoris.

Performance testing

Test example 1

DPPH radical scavenging Activity assay

The fermented oils prepared in examples and comparative examples were sampled, diluted to a concentration of 20% with pure water, and the DPPH radical scavenging activity in the samples was measured as follows:

Accurately sucking 1.0mL of sample solution, fully mixing the sample solution with the 2.0mLDPPH working solution prepared at present, carrying out light-proof reaction at room temperature for 30min, measuring the absorbance B at 517nm, and simultaneously measuring the absorbance C of 2mL of 95% ethanol solution and 1mL of sample solution and the absorbance A of 2mLDPPH working solution and 1mL of sample solvent. Vitamin C is used as positive control, water is used as negative control, each concentration of each sample is repeated for 3 times, average value is taken, DPPH free radical clearance rate is calculated,

The DPPH radical scavenging activity is shown in table 5:

TABLE 5

Analysis of results:

1. it can be seen from examples 1 to 5 that the DPPH radical scavenging rates of examples 1 to 5 show a certain fluctuation trend, but the overall fluctuation range is relatively small when the components and the addition amounts of the compound oil are changed;

2. It can be seen from examples 1 and examples 6 to 7 that when example 1 was used with oat as cereal or example 7 was used with a mixture of corn and buckwheat as cereal, the DPPH radical scavenging rates of examples 1 and 7 were 77.84% and 78.19%, respectively, but it can be seen from observation that example 6, when example 6 was prepared with oat, corn and buckwheat at the same time, the DPPH radical scavenging rate of example 6 reached 81.98%, and it can be seen that the effect obtained from example 6 was far more than the simple superposition of examples 1 and 7;

3. It can be seen from examples 1 and examples 8 to 9 that when the mass ratio of pichia pastoris to cereal is adjusted in examples 8 and 9, the DPPH radical scavenging rate of both products tends to decrease to some extent, and that the mass ratio of pichia pastoris to cereal in example 1 is the best mass ratio;

Further observing the examples 1 and 10-11, it can be seen that when the mass ratio of the fermentation liquor containing living bacteria to the compound oil is adjusted in the examples 10-11, the DPPH free radical scavenging rate of the examples 10-11 all generates a certain decreasing trend, and the mass ratio of the fermentation liquor containing living bacteria to the compound oil in the example 1 is the optimal mass ratio;

4. As can be seen from examples 1 and comparative examples 1 to 5, when any one of sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice bran oil is absent from the compound oil, the DPPH radical scavenging rate of comparative examples 1 to 5 all has a significant tendency to decrease, and any one of sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice bran oil is indispensable;

Further observing example 1 and comparative examples 6-10, it can be seen that, when any one of the sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice bran oil in the compound oil is replaced by other vegetable oil, the DPPH radical scavenging rate of comparative examples 6-10 all has a significant decreasing trend, and any one of the sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice bran oil is not replaceable;

5. As can be seen from the observation of example 1 and comparative examples 11 to 12, when pichia pastoris is replaced with other yeasts in comparative examples 11 to 12, the DPPH radical scavenging rate of comparative examples 11 to 12 is significantly decreased, and it is presumed that this phenomenon is caused by relatively less substances having radical scavenging ability which are metabolized after fermenting the complex oil by galactose yeast and sake yeast, thereby resulting in a decrease in DPPH radical scavenging rate of the fermented oil.

Test example two

Efficacy assay for promoting cell migration

The fermented oil prepared in the examples and comparative examples was sampled, diluted with pure water to a concentration of 5%, and the cell migration promoting effect of the samples was measured by the following method:

(1) Taking 3T3 cells in the logarithmic growth phase and with good growth condition, and preparing single cell suspension by digestion with pancreatin. Cells were seeded in 6-well plates, cell suspensions were adjusted to a cell concentration of 2E 5/mL, 2mL of complete medium was added per well, cells were incubated at 37 ℃ and 5% co ₂, after cells were nearly full, scratches were prepared, 200uL of gun tips were streaked evenly, then 2-3 times were washed with 2 mL/well PBS, suspended cells were washed off as much as possible, after washing, the blank set was replaced with complete medium containing 1% serum, the sample set contained test substance (1%) and complete medium containing 1% serum, cell migration was observed at 0-20h, and photographic recordings were made and mobility was calculated using ImageJ software.

The results of the cell migration promoting efficacy test for each example are shown in table 6:

TABLE 6

Sample of	Cell mobility (%)	Sample of	Cell mobility (%)
				Example 1	48.94	Comparative example 2	38.59
Example 2	48.37	Comparative example 3	39.35
				Example 3	49.78	Comparative example 4	38.43
Example 4	48.89	Comparative example 5	39.01
				Example 5	49.81	Comparative example 6	39.15
Example 6	52.10	Comparative example 7	39.27
				Example 7	48.39	Comparative example 8	39.38
Example 8	47.59	Comparative example 9	39.14
				Example 9	47.66	Comparative example 10	39.20
Example 10	47.91	Comparative example 11	38.23
				Example 11	47.38	Comparative example 12	37.44
Comparative example 1	38.72	Blank group	36.33

Analysis of results:

1. it can be seen from examples 1 to 5 that the cell mobilities of examples 1 to 5 exhibited a certain tendency to fluctuate, but the overall fluctuation range was relatively small when the components of the compound oil and the addition amounts thereof were changed;

2. It can be seen from examples 1 and examples 6 to 7 that when example 1 was used with oat as cereal or example 7 was used with a mixture of corn and buckwheat as cereal, the cell mobilities of examples 1 and 7 were 48.94% and 48.39%, respectively, but it can be seen from observation that example 6, when example 6 was used with oat, corn and buckwheat for preparation of fermentation broth, the cell mobility of example 6 reached 52.10%, and it can be seen that the effect achieved by example 6 was far superior to the simple superposition of examples 1 and 7;

3. It can be seen from examples 1 and examples 8 to 9 that when the mass ratio of pichia pastoris to cereal is adjusted in examples 8 and 9, the cell mobility of both cells tends to decrease to some extent, and that the mass ratio of pichia pastoris to cereal in example 1 is the best mass ratio;

Further examining examples 1 and 10-11, it can be seen that when the mass ratio of the fermentation broth containing living bacteria to the compound oil is adjusted in examples 10-11, the cell mobility of examples 10-11 all tends to decrease to some extent, and it can be seen that the mass ratio of the fermentation broth containing living bacteria to the compound oil in example 1 is the optimal mass ratio;

4. As can be seen from examples 1 and comparative examples 1 to 5, when any one of sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice bran oil is absent from the compound oil, the cell mobility of comparative examples 1 to 5 is significantly decreased, and any one of sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice bran oil is indispensable;

Further observing example 1 and comparative examples 6-10, it can be seen that, when any one of the sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice bran oil in the compound oil is replaced by other vegetable oil, the cell mobility of comparative examples 6-10 all has a significant decrease tendency, and any one of the sunflower seed oil, silybum marianum seed oil, olive fruit oil, shea butter and rice bran oil is not replaced;

5. As can be seen from the observation of example 1 and comparative examples 11 to 12, when comparative examples 11 to 12 were replaced with other yeasts, the cell mobility of comparative examples 11 to 12 was significantly decreased, and it is presumed that this phenomenon was caused by relatively less substances having an ability to promote cell migration, which were metabolized by galactose yeast and sake yeast after fermenting the complex oil, and thus resulted in a decrease in the cell mobility of the fermented oil.

Test example three

Stratum corneum moisture content test

4.1 Test methods

30 Healthy male or female subjects with ages of 18-40 years old are selected, and the experiment is carried out under the environment with constant temperature and humidity and the temperature (20-22 ℃ and the humidity of 40-60%). Before the experiment, the subject needs to sit still for 20min under the environment, after the environment reaches balance, a random, single-blind and control test is adopted, a blank control (pure water) and a test sample (for sampling the fermented oil prepared in the examples 1-5, using the pure water to dilute to a concentration of 5%) are respectively used on the left and right forearms of the subject according to a random principle, and the test part is smeared with a dose of (2.0+/-0.1) mg/cm ². The skin moisture content of the experimental part was measured 3 times by a tester using a Corneometer CM825 skin moisture content tester before and after 4 hours of sample application, and the average value was obtained for each subject, and the results are shown in Table 7:

TABLE 7

Sample of	Rate of change of moisture content of human skin/%	Sample of	Rate of change of moisture content of human skin/%
				Example 1	＋35.84	Example 4	＋36.41
Example 2	＋36.02	Example 5	＋35.90
				Example 3	＋36.12	Blank control group	＋4.52

Compared with the blank, the skin moisture content is obviously improved after the products obtained in the examples 1-5 are smeared on the skin, and the skin moisture content change rates of the other examples and the comparative examples are improved to a certain extent compared with the blank.

Claims

1. The fermented oil with the repairing, moisturizing and antioxidation effects is characterized in that the fermented oil with the repairing, moisturizing and antioxidation effects is obtained by fermenting compound oil by a fermentation broth containing living bacteria;

the fermentation liquor containing the pichia pastoris viable bacteria is prepared by fermenting grains by pichia pastoris;

the cereal is at least one selected from oat, corn and buckwheat;

the mass ratio of the pichia pastoris to the cereal is 1:3 to 8;

The compound oil consists of the following components in percentage by mass:

20-50% of sunflower seed oil;

1-3% of silybum marianum seed oil;

20-50% of olive fruit oil;

5-10% of shea butter;

10 to 20 percent of rice bran oil.

2. The fermented oil with repairing, moisturizing and antioxidant effects according to claim 1, wherein the compound oil consists of the following components in percentage by mass:

30-40% of sunflower seed oil;

1-3% of silybum marianum seed oil;

30-40% of olive fruit oil;

5-10% of shea butter;

12-18% of rice bran oil.

3. The fermented oil with repairing, moisturizing and antioxidant effects according to claim 1, wherein the grains are a mixture of oat, corn and buckwheat, and the mass ratio of oat, corn and buckwheat in the mixture of oat, corn and buckwheat is 1: 2-3: 0.8 to 1.5.

4. The fermented oil with repairing, moisturizing and antioxidant effects according to claim 1, wherein the grains are a mixture of oat, corn and buckwheat, and the mass ratio of oat, corn and buckwheat in the mixture of oat, corn and buckwheat is 1:2.5:1.2.

5. A method for producing the fermented oil having the effects of repairing, moisturizing and antioxidation according to any one of claims 1 to 3, characterized by comprising the steps of:

6. The method for preparing fermented oil with repairing, moisturizing and antioxidant effects according to claim 5, wherein the step 2 further comprises the following substeps:

Step A3: mixing the fermentation liquor containing living bacteria with the compound oil prepared in the step1, fermenting, centrifuging and collecting an oil phase to obtain the fermentation oil with the effects of repairing, preserving moisture and resisting oxidation.

7. Use of the fermented oil with moisturizing and antioxidant effects as set forth in any one of claims 1 to 4 in the preparation of cosmetics.

8. A cosmetic characterized by comprising 1 to 20% by weight of the fermented oil having the effects of repairing, moisturizing and antioxidation as defined in any one of claims 1 to 4.

9. The cosmetic product according to claim 8, wherein the cosmetic product is in the form of a patch, a paste, a liquid, a spray, a gel, an emulsion or a cream.