CN118703328A - Multifunctional biochemical analyzer - Google Patents

Abstract

The present disclosure provides a multifunctional biochemical analyzer, including: a sample loading module, a temperature control module, an oscillation module, a fluorescence detection module, an integrated control module and a housing, wherein the sample loading module completes sample loading, the temperature control module makes the ambient temperature of the sample reach a preset temperature range, the oscillation module oscillates and mixes the sample with a preset amplitude and a preset frequency, the fluorescence detection module performs real-time fluorescence detection on the sample, and the integrated control module is used to coordinate and control each module. The present disclosure can realize automatic sample mixing, precise temperature control and real-time fluorescence detection, and has the advantages of high integration and diverse functions.

Description

Multifunctional biochemical analyzer

Technical Field

The present disclosure relates to the technical field of biochemical detection, and in particular to a multifunctional biochemical analyzer.

Background Art

In the field of biochemical detection technology, existing analytical instruments face multiple challenges, including high operational complexity, strong dependence on environmental conditions, and insufficient sensitivity in low light or specific chemical detection. These limitations affect the efficiency and accuracy of experiments, especially when performing precise fluorescence detection.

Existing devices often lack effective temperature control and sample shaking functions, which are essential to ensure the uniformity and reproducibility of chemical reactions during experiments. Existing devices also lack integrated advanced functions such as automatic fluorescence detection, which also limits their application potential in the rapidly developing fields of biochemical research and clinical diagnostics.

Therefore, how to realize the multifunctional detection function of analytical instruments and improve their application scope and ease of use is a challenging task.

Summary of the invention

In view of the above problems, the embodiments of the present disclosure provide a multifunctional biochemical analyzer to solve the problems of difficulty in realizing multifunctional detection and small application scope.

The present invention provides a multifunctional biochemical analyzer, comprising: a sample loading module, used for loading samples; a temperature control module, arranged below the sample loading module, used for making the ambient temperature of the sample reach a preset temperature range; an oscillation module, arranged between the sample loading module and the temperature control module, connected to the sample loading module, used for oscillating and mixing the sample with a preset amplitude and a preset frequency; a fluorescence detection module, arranged above the sample loading module, used for performing real-time fluorescence detection on the sample; an integrated control module, arranged above the fluorescence detection module, electrically connected to the sample loading module, the temperature control module (2), the oscillation module and the fluorescence detection module, used for confirming that the sample loading module has completed sample loading, controlling the temperature control module to make the ambient temperature reach a preset temperature range, starting the oscillation module to oscillate and mix the sample, and controlling the fluorescence detection module to perform fluorescence detection on the sample; and a housing, wherein the sample loading module, the temperature control module, the oscillation module, the fluorescence detection module and the integrated control module are arranged inside the housing.

Optionally, the sample loading module includes: a pull-out slot, disposed in a preset hole on one side of the shell; and a handle, disposed at one end of the pull-out slot, for pulling the pull-out slot out of the shell to load the sample.

Optionally, the sample loading module further includes: a sample tray, which is clamped in the middle of the pull-out slot and is used for loading the sample.

Optionally, the sample loading module further includes: a buffer ring, disposed between the sample tray and the pull-out slot, for fixing the sample tray.

Optionally, the sample loading module further comprises: at least one sample slot, which is spaced apart in the sample tray and is used to place a reactor, and the reactor is loaded with the sample for sample reaction.

Optionally, the temperature control module includes: a temperature control element, arranged below the sample tray, for making the ambient temperature of the sample reach a preset temperature range; a heat dissipation rack, arranged at the bottom of the temperature control element, including a heat dissipation platform and a plurality of supporting legs, the heat dissipation platform being vertically connected to the plurality of supporting legs, and the temperature control element being arranged on the heat dissipation platform; a fan, arranged at the bottom of the supporting legs, for dissipating heat from the sample.

Optionally, the temperature control module includes: a temperature measuring element, which is arranged on the sample loading module, and is used to monitor the temperature of the sample and feed back the temperature of the sample to the integrated control module in real time.

Optionally, the fluorescence detection module includes: an excitation light source for outputting excitation light; a first support component, which is a triangular prism structure, including first, second and third quadrilateral planes adjacent to each other, the first quadrilateral plane of the first support component and the second quadrilateral plane are perpendicular to each other, the first quadrilateral plane of the first support component is arranged in parallel on the top of the sample loading module, and the second quadrilateral plane of the first support component is connected to the excitation light source; a second support component, which is a triangular prism structure, has the same size as the first support component, includes first, second and third quadrilateral planes adjacent to each other, the first quadrilateral plane of the second support component and the second quadrilateral plane are perpendicular to each other, and the first quadrilateral plane of the second support component is parallel to the first quadrilateral plane of the first support component. The third quadrilateral plane of the second supporting component overlaps with the third quadrilateral plane of the first supporting component to form a cubic structure; an excitation light filter is arranged at the excitation light output port of the excitation light source and fixed on the second quadrilateral plane of the first supporting component, and is used to filter out the excitation light in the first preset wavelength range; a dichroic mirror is arranged at the third quadrilateral plane of the first supporting component, and is used to reflect the light in the first wavelength range to the sample, excite the fluorescence of the sample, and transmit the fluorescence in the second preset wavelength range to the first quadrilateral plane of the second supporting component; an emission light filter is arranged on the first quadrilateral plane of the second supporting component, and is used to filter out the fluorescence in the third preset wavelength range; a photodetector is arranged on the top of the emission light filter, and is used to detect the fluorescence in the third preset wavelength range to image the sample.

Optionally, the photodetector is a complementary metal oxide semiconductor camera.

Optionally, it also includes a supporting connection module, including: a square supporting frame, arranged at the bottom of the shell, for supporting the temperature control module; a supporting platform, including a table top and a plurality of supporting legs, the integrated control module is arranged on the table top, and the oscillation module, sample loading module and fluorescence detection module are arranged in sequence in a preset space surrounded by the plurality of supporting legs.

At least one of the above technical solutions adopted in the embodiments of the present disclosure can achieve the following beneficial effects:

1. The multifunctional biochemical analyzer provided by the present disclosure integrates a sample loading module, a temperature control module, an oscillation module, a fluorescence detection module and an integrated control module in a housing, which can realize automatic sample mixing, precise temperature control and real-time fluorescence detection, and has a compact design, saves space, can significantly reduce costs, improve reaction efficiency, and has the advantages of high integration and diverse functions;

2. The present invention has strong compatibility, and the sample tray in the multifunctional biochemical analyzer is replaceable and adaptable to a variety of reactors to meet the requirements of temperature conditions and detection flux of a variety of biochemical reactions;

3. The integrated control module provided by the present disclosure is simple and easy to use, capable of flexible data processing, and can intuitively and conveniently control multiple functional modules through the touch screen. The operation process is simple, accurate and efficient, and supports local storage and remote transmission of data, which significantly improves the flexibility of data processing and sharing;

4. The photodetector provided in the present invention adopts a complementary metal oxide semiconductor (CMOS) camera. This imaging technology has low power consumption, high processing speed, high integration, low cost-effectiveness and high flexibility. The power consumption of CMOS sensors is much lower than that of CCD sensors, and they can provide faster image processing speeds. More functions can be integrated on the same chip, and they can provide faster image processing speeds. Each pixel can be read independently, which enhances the flexibility of the sensor and supports more reading modes.

BRIEF DESCRIPTION OF THE DRAWINGS

For a more complete understanding of the present disclosure and its advantages, reference will now be made to the following description taken in conjunction with the accompanying drawings, in which:

FIG1 schematically shows a three-dimensional structural diagram of a multifunctional biochemical analyzer provided in an embodiment of the present disclosure;

FIG2 schematically shows a schematic diagram of the line structure of a multifunctional biochemical analyzer provided in an embodiment of the present disclosure;

FIG3 schematically shows a schematic structural diagram of a sample loading module provided in an embodiment of the present disclosure;

FIG4 schematically shows a schematic structural diagram of a temperature control module provided in an embodiment of the present disclosure;

FIG5 schematically shows a three-dimensional structural diagram of a fluorescence detection module provided in an embodiment of the present disclosure;

FIG6 schematically shows a line structure diagram of a fluorescence detection module provided in an embodiment of the present disclosure;

FIG7 schematically shows a schematic diagram of the structure of an integrated control module provided in an embodiment of the present disclosure;

FIG8 schematically shows a schematic diagram of the line structure of the support connection module provided in an embodiment of the present disclosure.

Figure markings: 1-sample loading module; 101-pull-out slot; 102-sample; 103-buffer ring; 112-sample slot; 113-reactor; 2-temperature control module; 201-temperature control element; 202-heat sink; 203-fan; 204-temperature measuring element; 3-oscillation module; 4-fluorescence detection module; 401 excitation light source; 402-first support assembly; 403 second support assembly; 404-excitation light filter; 405 dichroic mirror; 406-emission light filter; 407-photodetector; 5-integrated control module; 501-single board computer; 502-touch screen; 503-power supply module; 504-communication interface; 6-housing; 7-support connection module; 701-square support frame; 702 table; 703-support leg; 704-support column.

DETAILED DESCRIPTION

Hereinafter, embodiments of the present disclosure will be described with reference to the accompanying drawings. However, it should be understood that these descriptions are exemplary only and are not intended to limit the scope of the present disclosure. In the following detailed description, for ease of explanation, many specific details are set forth to provide a comprehensive understanding of the embodiments of the present disclosure. However, it is apparent that one or more embodiments may also be implemented without these specific details. In addition, in the following description, descriptions of known structures and technologies are omitted to avoid unnecessary confusion of the concepts of the present disclosure.

The terms used herein are only for describing specific embodiments and are not intended to limit the present disclosure. The terms "include", "comprising", etc. used herein indicate the existence of the features, steps, operations and/or components, but do not exclude the existence or addition of one or more other features, steps, operations or components.

All terms (including technical and scientific terms) used herein have the meanings commonly understood by those skilled in the art, unless otherwise defined. It should be noted that the terms used herein should be interpreted as having a meaning consistent with the context of this specification, and should not be interpreted in an idealized or overly rigid manner.

FIG1 schematically shows a three-dimensional structural diagram of a multifunctional biochemical analyzer provided in an embodiment of the present disclosure;

In order to more clearly show the multifunctional biochemical analyzer of the embodiment of the present disclosure, FIG2 schematically shows a line structure diagram of a multifunctional biochemical analyzer provided by the embodiment of the present disclosure;

1 and 2 , the embodiment of the present disclosure provides a multifunctional biochemical analyzer, including a sample loading module 1, a temperature control module 2, an oscillation module 3, a fluorescence detection module 4, an integrated control module 5 and a housing 6. The sample loading module 1, the temperature control module 2, the oscillation module 3, the fluorescence detection module 4 and the integrated control module 5 are arranged inside the housing 6.

FIG3 schematically shows a schematic structural diagram of a sample loading module provided in an embodiment of the present disclosure.

As shown in Figure 3, the sample loading module 1 is used to load samples. As an optional embodiment, the sample loading module 1 includes a pull-out slot 101 and a handle. The pull-out slot 101 is arranged in a preset hole on one side of the shell 6; the handle is arranged at one end of the pull-out slot 101, which is used to pull the pull-out slot 101 out of the shell 6 to load the sample.

The design of the drawer slot 101 and the handle in the disclosed embodiment facilitates loading and unloading of samples, has a simple structure, and can improve detection efficiency.

As an optional embodiment, the sample loading module 1 further includes a sample tray 102, which is clamped in the middle of the pull-out slot 101 for loading samples. Specifically, the sample tray 102 of the embodiment of the present disclosure is detachable and replaceable, and is clamped in a preset hole of the pull-out slot to meet the requirements of the biochemical reaction on temperature conditions and detection flux.

In an illustrative embodiment, the material of the pull-out slot 101 is a non-spontaneous fluorescent material. The present disclosure does not specifically limit the material of the pull-out slot 101. For example, it can be polycarbonate (PC) or polylactic acid (PLA).

In an illustrative embodiment, the material of the sample tray 102 is a metal with high thermal conductivity. The present disclosure does not specifically limit the material of the sample tray 102. For example, the sample tray 102 may be at least one of metals such as copper and aluminum.

As an optional embodiment, the sample loading module 1 further includes a buffer ring 103 , which is disposed between the sample tray 102 and the draw-out slot 101 and is used to fix the sample tray 102 .

As an optional embodiment, the sample loading module 1 further includes at least one sample slot 112, which is arranged at intervals in the sample tray 102 and is used to place a reactor 113, and the reactor 113 is loaded with samples for sample reaction. The sample tray 102 of the disclosed embodiment is adapted to a variety of reactors 113 and can be applied to a variety of different types of biochemical experiments.

Specifically, the reactor 113 includes at least one of a PCR tube, a centrifuge tube, a multi-well plate, a culture dish, and a culture bottle.

FIG4 schematically shows a schematic structural diagram of a temperature control module provided in an embodiment of the present disclosure.

As shown in FIG. 4 , the temperature control module 2 is disposed below the sample loading module 1 and is used to make the ambient temperature of the sample reach a preset temperature range.

As an optional embodiment, the temperature control module 2 includes a temperature control element 201, a heat sink 202 and a fan 203. The temperature control element 201 is arranged below the sample tray 102, and is used to make the ambient temperature of the sample reach a preset temperature range; the heat sink 202 is arranged at the bottom of the temperature control element 201, and includes a heat dissipation platform and a plurality of supporting legs. The heat dissipation platform is vertically connected to the plurality of supporting legs, and the temperature control element 201 is arranged on the heat dissipation platform; the fan 203 is arranged at the bottom of the supporting legs, and is used to dissipate heat from the sample.

In an illustrative embodiment, the temperature control element 201 is at least one of a semiconductor refrigerator (Thermal Electronic Cooler, TEC), a printed circuit board (Printed circuit board, PCB) resistive heater, and the like.

As an optional embodiment, the temperature control module 2 includes a temperature measuring element 204 , which is disposed on the sample loading module 1 and is used to monitor the temperature of the sample and provide real-time feedback of the sample temperature to the integrated control module 5 .

Specifically, the temperature control module 2 adopts a closed-loop control system, and automatically controls the temperature control element 201 and the fan 203 through a feedback mechanism to achieve precise temperature control, wherein the temperature range is 4°C to 95°C, and the accuracy is ±0.1°C.

The oscillation module 3 is disposed between the sample loading module 1 and the temperature control module 2 , and is connected to the sample loading module 1 , and is used for oscillating and mixing the sample at a preset amplitude and a preset frequency.

In an illustrative embodiment, the oscillation module 3 adjusts the oscillation frequency and amplitude through programming, and the oscillation frequency ranges from 300 Hz to 1500 Hz, and the amplitude ranges from 0.5 mm to 5 mm.

FIG5 schematically shows a three-dimensional structural diagram of a fluorescence detection module provided in an embodiment of the present disclosure.

In order to more clearly show the structure of the fluorescence detection module provided by the embodiment of the present disclosure, FIG6 schematically shows a line structure diagram of the fluorescence detection module provided by the embodiment of the present disclosure.

5 and 6 , the fluorescence detection module 4 is disposed above the sample loading module 1 and is used for performing real-time fluorescence detection on the sample.

As an optional embodiment, the fluorescence detection module 4 includes an excitation light source 401 , a first support assembly 402 , a second support assembly 403 , an excitation light filter 404 , a dichroic mirror 405 , an emission light filter 406 and a photodetector 407 .

The excitation light source 401 is used to output excitation light.

In an illustrative embodiment, the excitation light source 401 is at least one of a laser, a halogen lamp, a mercury lamp, a xenon lamp, and a light emitting diode.

The first support component 402 is a triangular prism structure, including a first quadrilateral plane, a second quadrilateral plane and a third quadrilateral plane that are adjacent to each other. The first quadrilateral plane and the second quadrilateral plane of the first support component 402 are perpendicular to each other. The first quadrilateral plane of the first support component 402 is arranged parallel to the top of the sample loading module 1, and the second quadrilateral plane of the first support component 402 is connected to the excitation light source 401.

The second support component 403 is a triangular prism structure, which has the same size as the first support component 402, and includes first, second and third quadrilateral planes that are adjacent to each other. The first quadrilateral plane of the second support component 403 is perpendicular to the second quadrilateral plane, the first quadrilateral plane of the second support component 403 is parallel to the first quadrilateral plane of the first support component 402, and the third quadrilateral plane of the second support component 403 overlaps with the third quadrilateral plane of the first support component 402 to form a cubic structure.

The first support assembly 402 and the second support assembly 403 in the present disclosure are both triangular prism structures, which can be spliced to form a cubic structure. This structure has strong stability and is easy to be stably connected with various devices in the fluorescence detection module 4, thereby achieving the supporting function.

The excitation light filter 404 is disposed at the excitation light output port of the excitation light source 401 and fixed on the second quadrilateral plane of the first supporting component 402 , and is used for filtering out the excitation light within the first preset wavelength range.

The dichroic mirror 405 is disposed at the third quadrilateral plane of the first support assembly 402 , and is used to reflect light in the first wavelength range to the sample, stimulate fluorescence of the sample, and transmit fluorescence in the second preset wavelength range to the first quadrilateral plane of the second support assembly 403 .

The emission light filter 406 is disposed on the first quadrilateral plane of the second supporting assembly 403 and is used to filter out fluorescence within a third preset wavelength range.

The photodetector 407 is disposed on top of the emission filter 406 and is used to detect fluorescence in a third preset wavelength range to image the sample.

As an optional embodiment, the photodetector 407 is a complementary metal oxide semiconductor camera. Complementary metal oxide semiconductor cameras have low power consumption and are easy to carry; they can provide faster image processing speed, which is convenient for high-speed imaging (such as video shooting or continuous photography); they have high integration and can integrate more functions on the same chip, such as image processing and signal conversion, which simplifies the design of the camera system and helps to reduce costs; they have low production costs, and their production process is more similar to the standard semiconductor manufacturing process, and can be mass-produced at a lower cost; they are highly flexible, allowing each pixel to be read independently, enhancing the flexibility of the sensor and supporting more reading modes, such as window reading or multi-area reading.

In an illustrative embodiment, the photodetector 407 may also be at least one of a charge-coupled device (CCD) camera and an avalanche photo-diode (APD).

Specifically, the fluorescence detection module 4 in the embodiment of the present disclosure can detect at least one fluorescent dye among 4′,6-diamidino-2-phenylindole (DAPI), 5-carboxyfluorescein (FAM), Sulfo-Cyanine3 (CY3) and Sulfo-Cyanine3 (CY5).

FIG. 7 schematically shows a schematic diagram of the structure of an integrated control module provided in an embodiment of the present disclosure.

The integrated control module 5 is arranged above the fluorescence detection module 4, and is electrically connected to the sample loading module 1, the temperature control module 2, the oscillation module 3 and the fluorescence detection module 4, and is used to confirm that the sample loading module 1 has completed the sample loading, control the temperature control module 2 to make the ambient temperature reach a preset temperature range, start the oscillation module 3 to oscillate and mix the sample, and then control the fluorescence detection module 4 to perform fluorescence detection on the sample.

As shown in Figure 7, in an illustrative embodiment, the integrated control module 5 includes a single-board computer 501, a touch screen 502, a power supply module 503 and a communication interface 504. The touch screen 502 is arranged on the top of the shell 6 to facilitate touch interaction operations. The power supply module 503 is connected to the single-board computer 501, the touch screen 502 and the communication interface 504 for power supply.

In an illustrative embodiment, the integrated control module 5 supports remote operation and monitoring as well as cloud data processing and storage, and the integrated control module 5 is compatible with at least one of operating systems such as Windows, Linux, iOS, and Android.

The integrated control module 5 of the disclosed embodiment can intuitively and conveniently control multiple functional modules through the touch screen 502. The operation process is simple, accurate and efficient, which improves the flexibility of data processing and sharing.

FIG8 schematically shows a schematic diagram of the line structure of the support connection module provided in an embodiment of the present disclosure.

1 and 8 , as an optional embodiment, the multifunctional biochemical analyzer provided by the present disclosure further includes a supporting connection module 7, including: a square supporting frame 701, arranged at the bottom of the shell 6, for supporting the temperature control module 2; a supporting platform, including a table top 702 and a plurality of supporting legs 703, the integrated control module 5 is arranged on the table top 702, and the oscillation module 3, the sample loading module 1 and the fluorescence detection module 4 are arranged in sequence from bottom to top in a preset space surrounded by the plurality of supporting legs 703.

In an illustrative embodiment, four support columns 704 of the same size are provided at the four corners of the table 702 for stably supporting the integrated control module 5. The multifunctional biochemical analyzer disclosed in the present invention has a compact design, saves space, has a high degree of integration, can significantly reduce costs, and improve reaction efficiency.

The multifunctional biochemical analyzer provided by the present disclosure can be used for nucleic acid amplification, enzyme kinetics detection, cell and bacterial culture, etc. The following are specific embodiments of the present disclosure:

Embodiment 1

Real-time fluorescence quantitative polymerase chain reaction (qPCR), including steps S101 to S104.

Step S101, sample preparation and loading: prepare 170 μL PCR reaction mixture, including 1×Mix, 300 nM forward primer, 300 nM reverse primer, 200 nM probe, 1 μL DNA template. One end of the probe is modified with a BHQ group, and the other end is modified with a FAM group. Add the PCR reaction mixture to 8 rows of tubes, 20 μL per tube, pull out the pull-out slot 101, and place the 8 rows of tubes in the sample tray 102.

Step S102, temperature control setting: make the ambient temperature of the sample reach 95°C, keep it at 95°C for 5 minutes; continue to keep it at 95°C for 15 seconds; make the ambient temperature of the sample reach 55°C, keep it at 55°C for 30 seconds; make the ambient temperature of the sample reach 2°C, keep it at 2°C for 0 seconds, and repeat the above operation 40 times.

Step S103, fluorescence detection: the wavelength of the excitation light filter 404 is 485nm±10nm, the wavelength of the dichroic mirror 405 is 500nm, and the wavelength of the emission light filter 406 is 520nm±10nm. The fluorescence detection module 5 monitors the changes of the fluorescence signal in the PCR process in real time, which is recorded by the photodetector 407 and converted into digital data.

Step S104, data analysis: the analysis software built into the integrated control module 5 processes the collected data, automatically draws the amplification curve and calculates the threshold cycle (Ct) value, and the data results are intuitively displayed through the touch screen 502, providing clear and easy-to-interpret experimental data.

Embodiment 2

Single cell enzyme kinetics determination, including steps S201 to S205.

Step S201, sample preparation and loading: dilute the K562 cell sample and transfer it to a 384-well plate so that each well contains 0 or 1 cell; prepare a reaction solution, including a cell lysis agent, fluorescein di-β-D-galactoside (FDG) and a buffer solution; add the reaction liquid to the 384-well plate, pull out the pull-out slot 101, and place the 384-well plate in the sample tray 102.

Step S202, temperature control setting: make the ambient temperature of the sample reach 37°C and maintain it at this temperature for 2 hours.

Step S204, oscillation setting: oscillate the sample at a vibration frequency of 300 Hz and an amplitude of 1 mm to ensure that the K562 cells are fully lysed, and the β-galactosidase in the cells is released to mix with FDG and catalyze FDG to generate a fluorescent signal.

Step S205, fluorescence detection: the wavelength of the excitation light filter 404 is 485nm±10nm, the wavelength of the dichroic mirror 405 is 500nm, and the wavelength of the emission light filter 406 is 520nm±10nm. The fluorescence detection system 5 monitors the changes of the fluorescence signal in the enzyme kinetics process in real time, and the photodetector 407 records the fluorescence signal every 10 minutes and converts it into digital data.

Step S206, data analysis: the analysis software built into the integrated control module 5 processes the collected data, and the data results are intuitively displayed through the touch screen 502, providing clear and easy-to-interpret experimental data.

It will be appreciated by those skilled in the art that the features described in the various embodiments and/or claims of the present disclosure may be combined and/or combined in various ways, even if such combinations and/or combinations are not explicitly described in the present disclosure. In particular, the features described in the various embodiments and/or claims of the present disclosure may be combined and/or combined in various ways without departing from the spirit and teachings of the present disclosure. All of these combinations and/or combinations fall within the scope of the present disclosure.

Although the present disclosure has been shown and described with reference to specific exemplary embodiments of the present disclosure, it should be understood by those skilled in the art that various changes in form and details may be made to the present disclosure without departing from the spirit and scope of the present disclosure as defined by the appended claims and their equivalents. Therefore, the scope of the present disclosure should not be limited to the above-mentioned embodiments, but should be determined not only by the appended claims, but also by the equivalents of the appended claims.

Claims (10)

1. A multifunctional biochemical analyzer, characterized in that it comprises: A sample loading module (1), used for loading samples; A temperature control module (2), arranged below the sample loading module (1), and used to make the ambient temperature of the sample reach a preset temperature range; an oscillation module (3), disposed between the sample loading module (1) and the temperature control module (2), connected to the sample loading module (1), and used for oscillating and mixing the sample at a preset amplitude and a preset frequency; A fluorescence detection module (4), arranged above the sample loading module (1), and used for performing real-time fluorescence detection on the sample; An integrated control module (5) is arranged above the fluorescence detection module (4) and is electrically connected to the sample loading module (1), the temperature control module (2), the oscillation module (3) and the fluorescence detection module (4), and is used to confirm that the sample loading module (1) has completed sample loading, control the temperature control module (2) to make the ambient temperature reach a preset temperature range, start the oscillation module (3) to oscillate and mix the sample, and then control the fluorescence detection module (4) to perform fluorescence detection on the sample; A shell (6), wherein the sample loading module (1), the temperature control module (2), the oscillation module (3), the fluorescence detection module (4) and the integrated control module (5) are arranged inside the shell (6). 2. The multifunctional biochemical analyzer according to claim 1, characterized in that the sample loading module (1) comprises: A drawing slot (101) is provided in a preset hole on one side surface of the housing (6); A handle is provided at one end of the draw-out groove (101) and is used for pulling the draw-out groove (101) out of the housing (6) to load the sample. 3. The multifunctional biochemical analyzer according to claim 2, characterized in that the sample loading module (1) further comprises: The sample tray (102) is clamped in the middle of the drawer slot (101) and is used to load the sample. 4. The multifunctional biochemical analyzer according to claim 3, characterized in that the sample loading module (1) further comprises: A buffer ring (103) is provided between the sample tray (102) and the drawing groove (101) and is used to fix the sample tray (102). 5. The multifunctional biochemical analyzer according to claim 3, characterized in that the sample loading module (1) further comprises: At least one sample slot (112) is spaced apart in the sample tray (102) and is used to place a reactor (113). The reactor (113) is loaded with the sample for sample reaction. 6. The multifunctional biochemical analyzer according to claim 1, characterized in that the temperature control module (2) comprises: A temperature control element (201), disposed below the sample tray (102), and used to make the ambient temperature of the sample reach a preset temperature range; A heat dissipation frame (202), arranged at the bottom of the temperature control element (201), comprising a heat dissipation platform and a plurality of supporting legs, the heat dissipation platform being vertically connected to the plurality of supporting legs, and the temperature control element (201) being arranged on the heat dissipation platform; A fan (203) is provided at the bottom of the supporting legs and is used to dissipate heat from the sample. 7. The multifunctional biochemical analyzer according to claim 1, characterized in that the temperature control module (2) comprises: The temperature measuring element (204) is arranged on the sample loading module (1) and is used to monitor the temperature of the sample and to provide real-time feedback of the temperature of the sample to the integrated control module (5). 8. The multifunctional biochemical analyzer according to claim 1, characterized in that the fluorescence detection module (4) comprises: An excitation light source (401), used for outputting excitation light; The first support component (402) is a triangular prism structure, comprising a first quadrilateral plane, a second quadrilateral plane and a third quadrilateral plane adjacent to each other, the first quadrilateral plane and the second quadrilateral plane of the first support component (402) are perpendicular to each other, the first quadrilateral plane of the first support component (402) is arranged parallel to the top of the sample loading module (1), and the second quadrilateral plane of the first support component (402) is connected to the excitation light source (401); The second support component (403) is a triangular prism structure, having the same size as the first support component (402), and comprising first quadrilateral planes, second quadrilateral planes and third quadrilateral planes adjacent to each other, the first quadrilateral plane of the second support component (403) and the second quadrilateral plane are perpendicular to each other, the first quadrilateral plane of the second support component (403) and the first quadrilateral plane of the first support component (402) are parallel, and the third quadrilateral plane of the second support component (403) and the third quadrilateral plane of the first support component (402) overlap to form a cubic structure; an excitation light filter (404), disposed at an excitation light output port of the excitation light source (401), fixed on the second quadrilateral plane of the first supporting component (402), and used for filtering out excitation light within a first preset wavelength range; a dichroic mirror (405), disposed at the third quadrilateral plane of the first support component (402), for reflecting light in the first wavelength range to the sample, stimulating fluorescence of the sample, and transmitting fluorescence in a second preset wavelength range to the first quadrilateral plane of the second support component (403); An emission light filter (406), disposed on the first quadrilateral plane of the second support component (403), and used for filtering out fluorescence within a third preset wavelength range; The photodetector (407) is disposed on top of the emission light filter (406) and is used to detect the fluorescence in the third preset wavelength range to image the sample. 9. The multifunctional biochemical analyzer according to claim 1, characterized in that the photodetector (407) is a complementary metal oxide semiconductor camera. 10. The multifunctional biochemical analyzer according to claim 1, further comprising a supporting connection module (7), comprising: A square support frame (701), arranged at the bottom of the housing (6) and used to support the temperature control module (2); The support platform comprises a table top (702) and a plurality of support legs (703), wherein the integrated control module (5) is arranged on the table top, and the oscillation module (3), the sample loading module (1) and the fluorescence detection module (4) are arranged in sequence from bottom to top in a preset space surrounded by the plurality of support legs.