CN118684333B

CN118684333B - Water purification treatment methods

Info

Publication number: CN118684333B
Application number: CN202411171418.9A
Authority: CN
Inventors: 杨杨阳; 陈清; 陈忱
Original assignee: Suzhou Litree Purifying Technology Co Ltd
Current assignee: Suzhou Litree Purifying Technology Co Ltd
Priority date: 2024-08-26
Filing date: 2024-08-26
Publication date: 2024-12-10
Anticipated expiration: 2044-08-26
Also published as: CN118684333A

Abstract

The application relates to the technical field of water body treatment, in particular to a water body purification treatment method. The ecological restoration method comprises the steps of fixing a biological film in a water body, purifying the water body, preparing a modified biological film carrier by carrying out plasma modification on the surface of the biological film carrier by adopting plasma treatment, fixing active enzymes on the surface of the modified biological film carrier, which is modified by the plasma, so as to realize enzyme modification, preparing an enzyme modified biological film carrier, coating microbial suspension on the surface of the enzyme modified biological film carrier, and adhering and inoculating microorganisms on the surface of the enzyme modified biological film carrier, which is modified by the enzyme, by utilizing the affinity between the active enzymes and the microorganisms, so as to form a microorganism loading layer, thereby preparing the biological film, wherein the microorganisms are microorganisms capable of biodegrading organic pollutants in the water body.

Description

Purifying treatment method for water body

Technical Field

The application relates to the technical field of water body treatment, in particular to a water body purification treatment method.

Background

Industrial wastewater discharge, agricultural non-point source pollution, domestic wastewater discharge, landfill leachate, water hypoxia and organic matter putrefaction, exogenous pollution input and point source pollution, surface pollution source and underground pipe network problems, unreasonable water system structure, endogenous pollution and other problems can cause river channels to produce black and odorous water bodies, so that the enhancement of water body treatment and ecological restoration and the restoration of the health condition of a water ecological system are important.

In water, microorganisms are key participants in organic matter degradation, and can decompose complex organic matters into simpler inorganic matters, thereby playing roles in purifying and restoring water ecology. Therefore, a good growth space is provided for microorganisms, the metabolic mobility among the microorganisms is improved, the stable attachment and growth of the microorganisms are ensured, and the ecological restoration efficiency of the water body is improved.

Disclosure of Invention

Based on the method, the application provides a water purification treatment method. The water body restoration method provided by the application can provide good growth space for microorganisms and effectively improve the activity of the microorganisms by selecting the specific biological film so as to further improve the purification effect and the ecological restoration rate of the water body.

The application provides a purifying and treating method of a water body, which comprises the steps of ecological restoration, namely a biological film purifying step, wherein the biological film purifying step comprises the steps of fixing a biological film in the water body and purifying the water body;

the preparation steps of the biological film comprise:

Carrying out plasma modification on the surface of a biological film carrier by adopting plasma treatment to prepare a modified biological film carrier, wherein the temperature of the plasma treatment is 80-170 ℃, the plasma modification comprises etching and activating, and the plasma modification at least comprises the steps of applying plasma on the surface of the biological film carrier to etch and activate the surface of the biological film carrier so as to form a roughened surface and an active site;

Fixing active enzyme on the surface of the modified biological film carrier modified by plasma to realize enzyme modification and prepare the enzyme modified biological film carrier, wherein the fixing mode of the active enzyme comprises at least one of physical attachment, coupling connection and intermolecular interaction between the active enzyme and the active site;

And coating a microbial suspension on the enzyme modified surface of the enzyme modified biological film carrier, and adhering and inoculating the microorganisms to the enzyme modified surface of the enzyme modified biological film carrier by utilizing the affinity between the active enzyme and the microorganisms to form a microorganism loading layer, so as to prepare the biological film, wherein the microorganisms are microorganisms capable of biodegrading organic pollutants in the water body.

In one embodiment, the biofilm preparation step has one or more of the following features:

(1) In the step of performing plasma modification on the surface of the biological film carrier by adopting plasma treatment, the plasma treatment comprises oxygen plasma treatment, and the formed active sites comprise hydroxyl groups;

(2) In the step of carrying out plasma modification on the surface of the biological film carrier by adopting plasma treatment, the plasma treatment time is 8-15 s;

(3) The material of the biological film carrier comprises one or more of degradable polymers and natural fibers;

(4) The active enzyme comprises one or more of protease, amylase, laccase, cellulase, hemicellulase, peroxidase, lipoxygenase, phosphatase, urease and glucose oxidase;

(5) The microbial suspension includes a microbial strain including one or more of genus Dechloromonas, genus Agrimonia, genus steroidogacterium, genus Humicola and genus Comamonas.

In one embodiment, the preparation step of the biofilm comprises:

Washing and drying the biomembrane carrier, wherein the drying temperature is 30-60 ℃;

The preparation method comprises the steps of carrying out plasma modification on the surface of a biological film carrier by adopting plasma treatment at the temperature of 80-170 ℃ to prepare a modified biological film carrier, wherein the plasma treatment comprises oxygen plasma treatment, and the time of the plasma treatment is 8-15 s;

In one embodiment, the step of ecologically restoring further comprises one or more of a bank zone restoration, an ecological decontamination, an oxygenation treatment, and an aquatic plant planting step, the step of ecologically restoring the body of water having one or more of the following characteristics:

(1) The land restoration method comprises the steps of planting land plants;

(2) The step of oxygenation treatment comprises the steps of increasing dissolved oxygen in a water body in an aeration mode;

(3) The aquatic plant planting includes one or more of planting lotus roots and planting typha aquatic plants.

In one embodiment, the step of ecologically purifying comprises delivering a biological enzyme preparation to the body of water;

wherein the step of delivering a biological enzyme preparation into the body of water has one or more of the following characteristics:

(1) The adding amount of the biological enzyme preparation is 5g/m ³~8g/m³ according to the adding amount of each cubic meter of water body;

(2) The biological enzyme preparation comprises, by weight, 100 parts of complex enzyme, 40-60 parts of sodium polyacrylate, 10-18 parts of activated carbon, 5-10 parts of zeolite powder and 5-10 parts of bentonite.

In one embodiment, the method for purifying and treating the water body further comprises the steps of source control, sewage interception, endogenous treatment and repair and monitoring management which are sequentially carried out, wherein the step of ecological restoration is carried out after the step of endogenous treatment and before the step of repair and monitoring management.

In one embodiment, the endogenous remediation comprises one or more of garbage cleaning, flocculation sedimentation, and dredging, and the endogenous remediation has one or more of the following characteristics:

(1) The garbage cleaning comprises one or more of cleaning coastal garbage, cleaning seasonal fallen leaves, cleaning biological residues and cleaning floaters;

(2) The dredging comprises the step of cleaning the sediment.

In one embodiment, the step of flocculating settling includes adding a flocculant to the body of water;

Wherein the step of delivering a flocculant into the body of water has one or more of the following characteristics:

(1) The dosage of the flocculant is 95g/m ³~110g/m³ according to the dosage of each cubic meter of water body;

(2) The flocculant comprises, by weight, 100 parts of aluminum sulfate, 40-60 parts of polymeric ferric sulfate, 20-30 parts of ferric chloride, 20-30 parts of polyvinyl alcohol, 15-30 parts of amylase and 90-100 parts of activated carbon.

In one embodiment, the decontamination remediation process has one or more of the following features:

(1) The pollution control source interception step comprises the steps of inspecting and intercepting a sewage outlet of a water body so as to control the discharge of sewage from a source to the water body and reduce the pollution load entering the water body;

(2) The repair monitoring management step comprises the steps of periodically detecting the water bodies in a plurality of treatment and repair areas and the surrounding environment of the treatment and repair areas, and periodically inspecting and maintaining.

In one embodiment, the method further comprises the step of environmental investigation before the step of source control and pollution interception, wherein the method for purifying and treating the water body comprises the following steps of:

Dividing the water body into a plurality of treatment and restoration areas, detecting the water body in each treatment and restoration area, and investigating the surrounding environment and pollution source state of the treatment and restoration area to obtain environmental condition data;

Controlling source to intercept sewage, namely, inspecting and intercepting a sewage outlet of the water body so as to control the sewage to be discharged to the water body from the source and reduce pollution load entering the water body;

The method comprises the steps of garbage cleaning, flocculation sedimentation and dredging, wherein the garbage cleaning comprises one or more of coastal garbage cleaning, seasonal fallen leaves cleaning, biological residue cleaning and floating object cleaning, the flocculation sedimentation comprises the steps of adding a flocculating agent into a water body, the adding amount of the flocculating agent is 95g/m ³~110g/m³ in terms of adding amount per cubic meter of the water body, the flocculating agent comprises 100 parts of aluminum sulfate, 40-60 parts of polymeric ferric sulfate, 20-30 parts of ferric chloride, 20-30 parts of polyvinyl alcohol, 15-30 parts of amylase and 90-100 parts of activated carbon in parts by weight, and the dredging comprises the step of cleaning bottom mud;

The ecological restoration comprises the steps of carrying out a shore restoration, ecological purification, oxygenation treatment, aquatic plant planting and biological film purification in sequence, wherein the shore restoration comprises land plant planting, the ecological purification comprises the steps of adding a biological enzyme preparation into a water body, the adding amount of the biological enzyme preparation is 5g/m ³~8g/m³ based on the adding amount of each cubic meter of the water body, the biological enzyme preparation comprises 100 parts of complex enzyme, 40-60 parts of sodium polyacrylate, 10-18 parts of activated carbon, 5-10 parts of zeolite powder and 5-10 parts of bentonite in parts by weight, the oxygenation treatment comprises the steps of increasing dissolved oxygen in the water body in an aeration planting mode, and the aquatic plants comprise one or more of lotus roots and typha aquatic plant planting;

And (3) repairing and monitoring management, namely periodically detecting the water bodies in a plurality of treatment and repair areas and the surrounding environment of the treatment and repair areas, and periodically inspecting and maintaining.

The water purification treatment method provided by the application has the beneficial effects that at least:

Specific biofilms are used for water purification, wherein the biofilm preparation steps include plasma treatment, immobilization of active enzymes, and microorganism loading steps.

Plasma treatment is adopted to carry out plasma modification on the surface of the biological film carrier at a specific reaction temperature, and at the moment, the plasma acts on the surface of the biological film carrier to enable the surface of the biological film carrier to be subjected to physical etching and chemical activation so as to form a roughened surface and an active site, and the surface area and the biological attachment point of the biological film carrier are increased. On the one hand, the formation of the roughened surface can adjust the pore structure and permeability of the surface of the biological film carrier, which provides a richer space for free circulation of substances such as water, nutrient substances, oxygen and the like which promote the metabolic activity of microorganisms, and on the other hand, the increased surface area of the roughened surface and active sites formed by chemical activation can provide more and more stable attachment sites for stable attachment and growth of microorganisms and active enzymes on the carrier.

The active enzyme is further contacted with the modified biofilm carrier, and the active enzyme is immobilized on the surface of the modified biofilm carrier by utilizing the attachment of the active enzyme and biochemical reaction (such as coupling connection between the active enzyme and an active site and intermolecular interaction, wherein the intermolecular interaction is exemplified by induction force or hydrogen bond) in the preparation process. In the process, the introduction of the active enzyme molecules is beneficial to improving the biological activity of the carrier, at the moment, the affinity between the active enzyme molecules and microorganisms can promote the adhesion and growth of the microorganisms on the surface of the carrier, improve the inoculation speed of the microorganisms and the formation speed of the biological film, and simultaneously, the enzyme modified biological film carrier has better stability and adaptability when facing water quality fluctuation or environmental change so as to enhance the stability and environmental friendliness of the biological film. The steps of plasma treatment and active enzyme immobilization are sequentially carried out on the biological film carrier, so that the biological film carrier has higher surface area, more microorganism attachment points and higher biological activity, microorganisms can be efficiently attached and inoculated on the surface of the biological film carrier to form a high-biological-activity microorganism loading layer, and the formation process of the biological film can be accelerated.

Furthermore, the biological film is used for purifying water, microorganisms with higher biological activity are attached to the biological film carrier for propagation, substances such as water, nutrient substances, oxygen and the like which promote the metabolic activity of the microorganisms can freely circulate on the biological film carrier, and the biological film and the nutrient substances cooperate with each other, so that the biological film can quickly decompose complex organic substances into simpler inorganic substances through biological action, better purifying and ecological restoration effects of the water are achieved, and the ecological restoration efficiency of the water is further effectively improved.

Drawings

FIG. 1 is a process flow diagram of a method for preparing a biofilm provided in an embodiment of the application;

FIG. 2 is a process flow diagram of a method for purifying a body of water according to one embodiment of the present application;

FIG. 3 is an SEM image of the polylactic acid fiber biofilm carrier selected in the step (5-1) of the embodiment 1 of the present application;

FIG. 4 is an SEM image of an enzyme-modified biofilm carrier prepared in step (5-3) of example 1 of the present application;

FIG. 5 is an SEM image of a biofilm prepared in step (5-4) of example 1 of the present application;

Fig. 6 is an infrared spectrum of the polylactic acid fiber biofilm carrier before and after the plasma modification in example 1.

Detailed Description

The water body purifying and treating method of the present application is further fully and clearly described below with reference to the specific examples. The present application may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

In the water purification process, the microorganisms can effectively decompose organic pollutants so as to improve the water purification effect and the water purification capacity. The method mainly comprises the steps of (1) uniformly mixing a composite bacterial liquid with a microbial nitrogen source, adding a biological carrier, uniformly stirring, drying to prepare a microbial sewage treatment agent, and using the microbial sewage treatment agent for ecological restoration, wherein the method cannot provide a good growth space for microorganisms, the metabolic mobility among the microorganisms is poor, the stable attachment and growth of the microorganisms cannot be ensured, the purification treatment efficiency of a water body is low, and (2) the nylon elastic suspension filler is grafted with p-azidobenzoic acid on the surface for ecological restoration.

Based on the above, the first aspect of the application provides a purifying and treating method of a water body, the purifying and treating method of the water body comprises the steps of ecological restoration, wherein the ecological restoration comprises the steps of S405 and biological film purification, and the biological film purification comprises the steps of fixing biological films in the water body and purifying the water body;

the preparation steps of the biological film comprise:

b, carrying out plasma modification on the surface of the biological film carrier by adopting plasma treatment to prepare a modified biological film carrier, wherein the temperature of the plasma treatment is 80-170 ℃, the plasma modification comprises etching and activating, and the plasma modification at least comprises the steps of applying plasma on the surface of the biological film carrier to etch and activate the surface of the biological film carrier so as to form a roughened surface and an active site;

step c, fixing active enzyme on the surface of the modified biological film carrier subjected to plasma modification to realize enzyme modification, so as to prepare the enzyme modified biological film carrier, wherein the fixing mode of the active enzyme at least comprises at least one of physical attachment, coupling connection and intermolecular interaction between the active enzyme and the active site;

And d, coating a microorganism suspension on the enzyme modified surface of the enzyme modified biological film carrier, and adhering and inoculating the microorganism to the enzyme modified surface of the enzyme modified biological film carrier by utilizing the affinity effect between the active enzyme and the microorganism to form a microorganism loading layer, so as to prepare the biological film, wherein the microorganism is a microorganism capable of biodegrading organic pollutants in the water body.

The application uses a specific biological film for water purification, wherein the preparation steps of the biological film comprise the steps of plasma treatment, active enzyme immobilization and microorganism loading.

Plasma treatment is adopted to carry out plasma modification on the surface of the biological film carrier at a specific reaction temperature, and at the moment, the plasma acts on the surface of the biological film carrier to enable the surface of the biological film carrier to be subjected to physical etching and chemical activation so as to form a roughened surface and an active site, and the surface area and the biological attachment point of the biological film carrier are increased. On the one hand, the formation of the roughened surface can adjust the pore structure and permeability of the surface of the biological film carrier, which provides more plentiful space for free circulation of substances such as water, nutrient substances and oxygen which promote the metabolic activity of microorganisms in the pores, and on the other hand, the increased surface area of the roughened surface and active sites formed by chemical activation can provide more and more stable attachment sites for stable attachment and growth of microorganisms and active enzyme organisms on the carrier.

The active enzyme is further contacted with the modified biological film carrier, and the active enzyme is immobilized on the surface of the modified biological film carrier by utilizing the adhesion of the active enzyme and biochemical reaction (such as coupling connection between the active enzyme and an active site and intermolecular interaction, such as induction force or hydrogen bond) in the preparation process. In the process, the introduction of the active enzyme molecules is beneficial to improving the biological activity of the carrier, at the moment, the affinity between the active enzyme molecules and microorganisms can promote the adhesion and growth of the microorganisms on the surface of the carrier, improve the inoculation speed of the microorganisms and the formation speed of the biological film, and simultaneously, the enzyme modified biological film carrier has better stability and adaptability when facing water quality fluctuation or environmental change so as to enhance the stability and environmental friendliness of the biological film. The steps of plasma treatment and active enzyme immobilization are sequentially carried out on the biological film carrier, so that the surface area is relatively high, the microorganism attachment points are relatively large, and the biological film carrier has relatively high biological activity, so that microorganisms can be efficiently attached to the surface of the biological film carrier to form a microorganism loading layer with high biological activity, and the formation process of the biological film can be accelerated.

It will be appreciated that in step b, the temperature of the plasma treatment may be selected from any value between 80 ℃ and 170 ℃. Further, the temperature of the plasma treatment includes, but is not limited to, 80 ℃, 90 ℃, 95 ℃, 100 ℃, 110 ℃, 120 ℃, 130 ℃, 140 ℃, 150 ℃, 160 ℃, or 170 ℃.

It can be understood that when the water body is a black and odorous water body of a river channel, the step of purifying the biological film comprises fixing the biological film at the bottom or the side wall of the river channel to purify the water body.

In one example, the step of securing the biofilm to the underside or side of the body of water includes attaching an anchor to the bottom or side wall of the channel and installing the biofilm on the anchor.

In one example, the material of the biofilm carrier includes one or more of a degradable polymer and natural fibers.

The biological film carrier adopts one or more of degradable polymers and natural fibers, has larger specific surface area and good biocompatibility, and meanwhile, the material also has proper porosity and permeability, which can ensure the fluidity of water and the effective diffusion of pollutants, and can allow free circulation of water, nutrient substances, oxygen and the like so as to promote the metabolic activity of microorganisms, so as to optimize the growth environment of the microorganisms, enhance the biological affinity, ensure the stable attachment and growth of the microorganisms on the carrier, and further improve the treatment efficiency of the biological film. In addition, the biological film carrier of the degradable polymer and the natural fiber has good mechanical strength, chemical stability and biological inertia, and can resist chemical corrosion and biological erosion in water, so that microorganisms can be better attached to the carrier to reproduce and degrade organic pollutants through biological action to purify the water.

Illustratively, among the materials of the biofilm carrier, degradable polymers may be exemplified as polylactic acid materials, polyglycolic acid, polycaprolactone, polyhydroxybutyrate, and polybutylene succinate. By way of example, natural fibers may be exemplified by cotton fibers, hemp fibers, wool fibers, silk fibers, kapok fibers, coconut fibers.

It will be appreciated that in the present application, the surface of the biofilm carrier is etched and activated to form a roughened surface and an active site plasma. In addition, the frequency, power, gas flow rate and other processing parameters in the plasma processing process of the present application can be selected in a manner conventional to those skilled in the art. Illustratively, in plasma processing, the working gas selected includes one or more of oxygen, nitrogen, argon, hydrogen, and helium. In one example, in the step of plasma modifying the surface of the biofilm carrier with a plasma treatment, the plasma treatment comprises an oxygen plasma treatment and the active sites formed comprise hydroxyl groups.

In one example, the plasma processing apparatus is a plasma jet apparatus.

The coating mode in the step d can be selected from coating modes commonly used by those skilled in the art, and further, the coating modes include, but are not limited to, brushing, spraying and dipping.

Illustratively, the active enzyme may be exemplified by proteases, amylases, laccases, cellulases, hemicellulases, peroxidases, lipoxygenases, phosphatases, ureases, and glucose oxidases, for example.

Illustratively, the microbial suspension includes a microbial organism including one or more of Declomonas (Dechloromonas), agrimonia (Gemma-timonadaceae), steroid bacillus (Steroidobacteraceae), pythium (Saprospiraceae), and Comamonas.

The organic pollutants in the water body mainly comprise polycarboxylic acid compounds of humic acid and fulvic acid, chlorinated organic compounds, steroid compounds and petroleum hydrocarbon organic compounds. The microorganism can be biologically degraded with at least one of organic pollutants in the water body so as to ensure the purification effect of the water body.

Further, the plasma treatment time is 8 s-15 s.

The high-energy particles in the plasma treatment process bombard the surface of the biological film carrier, so that the surface of the biological film carrier is subjected to physical etching and chemical activation, and the characteristics of the surface of the biological film carrier are improved. Further limiting the working gas and the technological parameters of plasma treatment, the coarsening degree and the activation level of the surface of the biological film carrier can be controlled to increase the surface area and the biological attachment point of the biological film carrier, and the surface damage or the performance reduction caused by excessive treatment can be effectively avoided.

In one example, before the step of plasma modifying the surface of the biological film carrier by adopting plasma treatment, the method further comprises the step of cleaning and drying the biological film carrier.

In one example, the step a comprises the steps of cleaning and drying the biological film carrier, wherein the drying temperature is 30-60 ℃.

The cleaning and drying treatment of the biomembrane carrier can effectively remove dust, grease, microorganism residues and other impurities on the surface of the biomembrane carrier, so that high-energy particles in subsequent plasma treatment can directly act on the surface of the carrier to improve the surface roughness and active sites and increase the attachment points of the biomembrane.

Referring to fig. 1, in one example, the preparation steps of the biofilm include:

step a, cleaning and drying the biomembrane carrier, wherein the drying temperature is 30-60 ℃;

The method comprises the steps of b, carrying out plasma modification on the surface of a biological film carrier by adopting plasma treatment at the temperature of 80-170 ℃ to prepare a modified biological film carrier, wherein the plasma treatment body comprises oxygen plasma treatment, and the time of the plasma treatment is 8-15 s;

step c, fixing active enzyme on the surface of the modified biological film carrier subjected to plasma modification to realize enzyme modification, and preparing the enzyme modified biological film carrier, wherein the fixing mode of the active enzyme comprises at least one of physical attachment, coupling connection between the active enzyme and an active site and intermolecular interaction;

and d, coating a microorganism suspension on the surface of the enzyme modified biological film carrier modified by the enzyme, and inoculating the microorganism on the surface of the enzyme modified biological film carrier by utilizing the affinity between the active enzyme and the microorganism to form a microorganism loading layer so as to prepare the biological film, wherein the microorganism is a microorganism capable of biodegrading organic pollutants in the water body.

In one example, the step of biofilm decontamination comprises:

The preparation method comprises the steps of b, carrying out plasma modification on the surface of the biological film carrier by adopting plasma treatment at the temperature of 80-170 ℃ to prepare a modified biological film carrier, wherein the plasma treatment comprises oxygen plasma treatment, and the time of the plasma treatment is 8-15 s;

Coating a microbial suspension on the surface of the enzyme modified biological film carrier modified by enzyme, and inoculating the microorganism on the surface of the enzyme modified biological film carrier modified by enzyme by utilizing the affinity between the active enzyme and the microorganism to form a microorganism loading layer, wherein the microorganism is a microorganism capable of biodegrading organic pollutants in the water body;

and e, fixing the biological film in a water body, and purifying the water body.

In one example, the ecological restoration method further comprises:

Step S401, shore zone restoration, step S402, ecological purification, step S403, oxygenation treatment, step S404 and one or more of aquatic plant planting.

In one example, in step S401, the manner of shore repair includes planting land plants. Furthermore, the land band repairing mode comprises the step of recovering the natural purifying function of the water body through grass planting ditches and an ecological bank protection mode.

In one example, in step S402, the step of ecologically purifying includes delivering a biological enzyme formulation into the body of water. The addition of the bio-enzyme preparation can accelerate the biodegradation process of the organic pollutants to catalyze the conversion of the pollutants into harmless or more biologically treatable substances, thereby reducing harmful chemical substances in the water body.

In order to fully exert the biodegradation and purification effects of the biological enzyme preparation, the biological enzyme preparation can effectively decompose organic pollutants in the water body, and meanwhile, the resource waste caused by the too high concentration can be avoided. In one example, the biological enzyme preparation is added in an amount of 5g/m ³~8g/m³ per cubic meter of water. Further, the dosage of the biological enzyme preparation includes, but is not limited to 5 g/m³、5.5 g/m³、6 g/m³、6.5 g/m³、7 g/m³、7.5 g/m³、8 g/m³.

In one example, the biological enzyme preparation comprises, by weight, 100 parts of complex enzyme, 40-60 parts of sodium polyacrylate, 10-18 parts of activated carbon, 5-10 parts of zeolite powder, and 5-10 parts of bentonite.

In the present application, the complex enzyme means a preparation comprising a plurality of enzymes, and illustratively, the complex enzyme includes at least two of protease, lipase, amylase, cellulase, lignin enzyme and pectase. The application can effectively catalyze the biodegradation of pollutants by optimizing the components of the biological enzyme preparation, wherein the composite enzyme can provide adsorption sites for the composite enzyme, improve the stability and bioavailability of the composite enzyme, and further cooperate with the sodium polyacrylate, so that the viscosity of the biological enzyme preparation can be effectively enhanced, the biological enzyme preparation can be uniformly distributed and can be attached to the surfaces of the pollutants. In addition, zeolite powder and bentonite are selected as fillers, so that on one hand, heavy metal ions and ammonia nitrogen can be fixed and adsorbed, and on the other hand, the suspension property and durability of the biological enzyme preparation can be enhanced. Therefore, the biological enzyme preparation can effectively decompose pollutants, and the components are synergistic, so that the performance and the water purification effect of the complex enzyme can be optimized. Further, the parts by weight of sodium polyacrylate include, but are not limited to, 40 parts, 41 parts, 45 parts, 46 parts, 48 parts, 50 parts, 55 parts, or 60 parts. The parts by weight of activated carbon include, but are not limited to, 10 parts, 11 parts, 12 parts, 15 parts, or 18 parts. The weight parts of the zeolite powder include, but are not limited to, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts. The parts by weight of bentonite include, but are not limited to, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, or 10 parts.

In one example, in step S403, the step of oxygenation treatment comprises increasing dissolved oxygen in the body of water by means of aeration. The increase of dissolved oxygen can accelerate the degradation speed of pollutants and improve the self-cleaning capability of the water body.

In one example, step S404, the step of growing the aquatic plants includes growing lotus root, typha aquatic plants in a body of water. The aquatic plants can be planted to purify the water body and improve the water quality through the absorption, adsorption and decomposition of the plants.

In one example, the ecological restoration includes the steps of:

And S401, restoring the bank zone, namely restoring the natural purification function of the water body in a grass planting ditch and ecological bank protection mode.

And S402, ecological purification, namely adding a biological enzyme preparation into the water body, wherein the adding amount of the biological enzyme preparation is 5g/m ³~8g/m³ based on the adding amount of each cubic meter of the water body, and the biological enzyme preparation comprises, by weight, 100 parts of complex enzyme, 40-60 parts of sodium polyacrylate, 10-18 parts of activated carbon, 5-10 parts of zeolite powder and 5-10 parts of bentonite.

And step S403, oxygenation treatment, namely increasing the dissolved oxygen in the water body in an aeration mode.

And S404, planting aquatic plants, namely planting lotus roots and typha aquatic plants in the water body.

The method comprises the steps of S405, fixing a biological film below or at the side of a water body, purifying the water body, wherein the preparation step of the biological film comprises the steps of a, cleaning and drying the biological film carrier, wherein the drying temperature is 30-60 ℃, b, performing plasma modification on the surface of the biological film carrier by adopting plasma treatment at 80-170 ℃ to prepare a modified biological film carrier, wherein the plasma treatment comprises oxygen plasma treatment for 8-15 seconds, the plasma modification comprises etching and activating, the plasma modification at least comprises the steps of enabling plasma to act on the surface of the biological film carrier, enabling the surface of the biological film carrier to be etched and activated to form a roughened surface and an active site, the active site comprises a hydroxyl group, C, fixing an active enzyme on the surface of the modified biological film carrier by using the plasma to realize enzyme modification, preparing the enzyme modified biological film carrier, the fixing mode of the active enzyme comprises the physical attachment of the enzyme, the active enzyme and the active enzyme, the biological film carrier and the biological film carrier are connected with one another by using a coupling mode of the enzyme, and the microbial carrier can be coated with a microbial carrier, and the microbial carrier can be subjected to at least one of the microbial carriers, and the microbial carriers can be subjected to the coupling, and the microbial carriers can be prepared.

In one example, the method for purifying and treating the water body further comprises a step S20 and a source control and sewage interception step S30, an endogenous treatment step S50 and a repairing and monitoring management step which are sequentially carried out, wherein the ecological repairing step is carried out after the endogenous treatment step and before the repairing and monitoring management step.

In one example, before the step of controlling the source to intercept the pollution, the method further comprises the step of environmental investigation:

And S10, dividing the water body into a plurality of treatment and restoration areas, detecting the water body in each treatment and restoration area, and investigating the surrounding environment and pollution source states of the treatment and restoration areas to acquire environmental condition data.

In one example, in step S20, the step of controlling the source to intercept the wastewater includes performing an interrogation interception of a wastewater discharge outlet of the body of water to control discharge of the wastewater from the source to the body of water.

In one example, in step S30, the endogenous remediation includes one or more of step S301, garbage cleaning, step S302, flocculation sedimentation, step S303, dredging.

In one example, in step S301, the trash cleaning includes one or more of cleaning coastal trash, cleaning seasonal fallen leaves, cleaning biological residues, and cleaning floats. The method can clean the coastal garbage and the biological residues and floaters such as seasonal fallen leaves, aquatic plants and the like contained in the water body, and can effectively avoid secondary pollution caused by decay of the garbage.

In one example, in step S302, the step of flocculating settling includes throwing a flocculant into the body of water.

In one example, the flocculant is delivered in an amount of 95g/m ³~110g/m³ per cubic meter of water. The dosage of the flocculant is limited, so that flocculation effect in the water treatment process can be ensured, and fine particles suspended in water are promoted to be aggregated into larger flocculation, so that suspended solids, colloid substances and certain soluble substances in the water are removed. Further, the dosage of the flocculant includes, but is not limited to, 95g/m ³、100 g/m³、105 g/m³、108 g/m³ or 110g/m ³.

In one example, the flocculant comprises, in parts by weight, 100 parts of aluminum sulfate, 40-60 parts of polymeric ferric sulfate, 20-30 parts of ferric chloride, 20-30 parts of polyvinyl alcohol, 15-30 parts of amylase, and 90-100 parts of activated carbon. Among the above components, aluminum sulfate, polymeric ferric sulfate and ferric chloride are used as inorganic flocculant, which can provide a great amount of positive charges to neutralize the negative charges suspended in the water body so as to promote aggregation and sedimentation of particles, and in addition, the addition of polyvinyl alcohol can form larger flocs through bridging action so as to improve the structure and stability of the flocs and make the flocs easier to precipitate and separate. In addition, the amylase and the activated carbon can be added to reduce the chemical oxygen demand and the biological oxygen demand of the water body and improve the biodegradability of the water body, and can adsorb organic matters, pigments and peculiar smell in the water.

Further, the parts by weight of polymeric ferric sulfate include, but are not limited to, 40 parts, 41 parts, 45 parts, 50 parts, 55 parts, or 60 parts. The parts by weight of ferric chloride include, but are not limited to, 20 parts, 21 parts, 22 parts, 25 parts, 28 parts, or 30 parts. The parts by weight of polyvinyl alcohol include, but are not limited to, 20 parts, 21 parts, 22 parts, 25 parts, 28 parts, or 30 parts. The parts by weight of amylase include, but are not limited to, 15 parts, 16 parts, 18 parts, 19 parts, or 20 parts. The parts by weight of activated carbon include, but are not limited to, 90 parts, 91 parts, 92 parts, 95 parts, 98 parts, or 100 parts.

In one example, in step S303, the dredging comprises cleaning sediment. The dredging method is adopted to clean the sediment, so that the endogenous pollution load can be rapidly reduced.

In one example, in step S50, the step of repairing, monitoring and managing includes periodically detecting and periodically inspecting and maintaining the water body of the plurality of treatment and repair areas and the surrounding environment of the treatment and repair areas.

Referring to fig. 2, in one example, a method of purifying a body of water includes the steps of:

and S10, environmental investigation, namely dividing the water body into a plurality of treatment and restoration areas, detecting the water body in each treatment and restoration area, and investigating the surrounding environment and pollution source states of the treatment and restoration areas to acquire environmental condition data.

And S20, controlling the source to intercept the sewage, namely, inspecting and intercepting the sewage outlet of the water body so as to control the discharge of the sewage from the source to the water body.

Step S30, endogenous governance, comprising the following steps:

Step S301, cleaning garbage, namely cleaning coastal garbage, cleaning seasonal fallen leaves, cleaning biological residues and cleaning floaters;

Step S302, flocculating settling, namely adding a flocculating agent into the water body, wherein the adding amount of the flocculating agent is 95g/m ³~110g/m³ based on the adding amount of each cubic meter of the water body, and the flocculating agent comprises, by weight, 100 parts of aluminum sulfate, 40-60 parts of polymeric ferric sulfate, 20-30 parts of ferric chloride, 20-30 parts of polyvinyl alcohol, 15-30 parts of amylase and 90-100 parts of activated carbon;

And S303, dredging, namely cleaning the bottom mud.

Step S40, ecological restoration, comprising the following steps:

The method comprises the steps of S405, purifying a biological film, namely fixing the biological film below or on the side of the water body, and purifying the water body, wherein the preparation step of the biological film comprises the steps of a, cleaning and drying the biological film carrier, wherein the drying temperature is 30-60 ℃, b, performing plasma modification on the surface of the biological film carrier by adopting plasma treatment at 80-170 ℃ to prepare a modified biological film carrier, wherein the plasma treatment comprises oxygen plasma treatment for 8-15 seconds, the plasma modification comprises etching and activating, the plasma modification at least comprises the steps of enabling plasma to act on the surface of the biological film carrier, enabling the surface of the biological film carrier to be etched and activated to form a roughened surface and an active site, the active site comprises hydroxyl groups, C, fixing an active enzyme on the surface of the modified biological film carrier by using plasma modification to prepare the enzyme modified biological film carrier, the active enzyme is fixed on the surface of the biological film carrier by using a coupling mode between the active enzyme and the surface of the biological film carrier, and the biological film is modified by using an interaction mode of at least one of the carrier, and a microbial enzyme is coated by using a coupling mode, and the biological enzyme is coated on the surface of the carrier.

And S50, repairing and monitoring management, namely periodically detecting the water bodies in a plurality of treatment and repair areas and the surrounding environment of the treatment and repair areas, and periodically inspecting and maintaining.

The application improves the self-cleaning capability of the water body by recovering the natural form and the biological diversity near the water body, increases the dissolved oxygen in the water body by aeration treatment to promote the decomposition of organic matters in the water and reduce the generation of black and odorous substances, and additionally carries out the steps of removing sediment and sundries in river channels and lakes, thereby improving the transparency of the water body and reducing the odor influence caused by the release of pollutants in bottom mud on the water body. The specific biological enzyme preparation is selected to carry out oxidative decomposition and chemical reaction on organic matters in the water body so as to further reduce the pollutant content of the water body, accelerate the decomposition and degradation of the organic matters and improve the odor treatment effect on the black and odorous water body, and meanwhile, the specific biological membrane is selected to adsorb, decompose and convert the organic matters so as to degrade the organic matters, thereby improving the quality of the water body, and maintaining the improvement of the water quality for a long time so as to realize ecological restoration. The application provides a water body system purifying method which can comprehensively treat and control the water body so as to maintain the excellent level of water quality.

In one example, the body of water is a body of river black and odorous water.

The following examples are further specific to illustrate the application in detail. It is also to be understood that the following examples are given solely for the purpose of illustration and are not to be construed as limitations upon the scope of the application, as many insubstantial modifications and variations are within the scope of the application as would be apparent to those skilled in the art in light of the foregoing disclosure. The specific process parameters and the like of the following examples are also merely examples of suitable ranges, i.e., those skilled in the art can make a suitable selection from the description herein without necessarily limiting the specific values of the examples.

Example 1

The embodiment 1 of the application provides a purification treatment method of a river black and odorous water body, which comprises the following steps of river environment investigation, source control and pollution interception, endogenous treatment, ecological restoration and restoration monitoring management.

Dividing the black and odorous water body river channel into a plurality of treatment and restoration areas, detecting the water body in each treatment and restoration area, and investigating the surrounding environment and pollution source states of the treatment and restoration areas to acquire environmental condition data.

The method comprises the steps of controlling source and intercepting sewage, namely, performing investigation and interception on drain outlets on two sides of a river channel, controlling the discharge of sewage from the source to the water body of the river channel, and reducing the pollution load entering the river channel.

The method comprises the steps of (1) cleaning coastal garbage, reducing sources of pollutants, (2) cleaning biological residues and floaters such as seasonal fallen leaves and aquatic plants, avoiding secondary pollution caused by decay of the biological residues, and (3) adding a flocculating agent into a water body in a restoration area, wherein the adding amount of the flocculating agent is 95g/m ³ in terms of adding amount of each cubic meter of the water body, and the flocculating agent comprises, by weight, 100 parts of aluminum sulfate, 40 parts of polymeric ferric sulfate, 20 parts of ferric chloride, 20 parts of polyvinyl alcohol, 15 parts of amylase and 90 parts of active carbon, and (4) dredging, and cleaning river sediment by adopting a dredging method, so that the endogenous pollution load is quickly reduced.

Restoring the natural purification function of the water body by a grass planting ditch and an ecological bank protection mode; (2) ecological purification, namely, putting a biological enzyme preparation into a river water body, wherein the putting amount of the biological enzyme preparation is 5g/m ³ according to the putting amount of each cubic meter of water body; the biological enzyme preparation comprises 100 parts of compound enzyme (comprising lipase, amylase and cellulase in a mass ratio of 1:1:1), 40 parts of sodium polyacrylate, 10 parts of active carbon, 5 parts of zeolite powder and 5 parts of bentonite in parts by weight, (3) oxygenation treatment, which is to increase dissolved oxygen in the water body in an aeration mode, accelerate degradation speed of pollutants and improve self-cleaning capability of the water body, (4) aquatic plant planting, which is to plant lotus root and typha aquatic plants, purify the water body and improve water quality through absorption, adsorption and decomposition of plants, (5) biological membrane purification, which is to firmly attach to the bottom or side wall of a river channel by using an anchor device, install the biological membrane on the anchor device, reproduce microorganisms on a carrier and degrade organic pollutants through biological action, and purify the water body, wherein the biological membrane preparation step comprises the steps of (5-1) cleaning the biological membrane carrier made of polylactic acid materials, drying the cleaned biological membrane carrier at 60 ℃, drying time of 8 ℃ and plasma treatment in a carrier treatment area by using plasma treatment device, wherein the biological membrane is subjected to plasma treatment in a carrier treatment area by using a plasma treatment device, the method comprises the steps of (1) preparing a modified biological film carrier by spraying active enzyme (the active enzyme comprises cellulase, amylase and protease with the mass ratio of 1:1:1) on the surface of the modified biological film carrier, utilizing the physical attachment of the active enzyme, coupling connection or intermolecular interaction between the active enzyme and the active site to fix the active enzyme on the surface of the modified biological film carrier, further enhancing the biological affinity, preparing the enzyme modified biological film carrier, and (5-4) inoculating cultured microorganisms on the enzyme modified biological film carrier in a microorganism incubator, wherein the temperature of the plasma treatment is 80 ℃, the plasma treatment time is 15s, and the step (5-3) comprises spraying active enzyme (the active enzyme comprises the cellulase, the amylase and the protease with the mass ratio of 1:1:1) on the surface of the enzyme modified biological film carrier, utilizing the coupling connection or intermolecular interaction between the active enzyme and the active site to fix the active enzyme on the surface of the modified biological film carrier, and utilizing the affinity between the active enzyme and the active enzyme to prepare the microbial film carrier.

The repair monitoring management step comprises the steps of periodically detecting the water bodies in a plurality of treatment and repair areas and the surrounding environment of the treatment and repair areas, and manually and periodically inspecting and maintaining.

Comparative example 1

The application provides a purification treatment method of a river black and odorous water body, which comprises the following steps of river channel environment investigation, source control and pollution interception, endogenous treatment, ecological restoration and restoration monitoring management.

Restoring the natural purification function of the water body by a grass planting ditch and an ecological bank protection mode; (2) ecological purification, namely, putting a biological enzyme preparation into a river water body, wherein the putting amount of the biological enzyme preparation is 5g/m ³ according to the putting amount of each cubic meter of water body; the biological enzyme preparation comprises 100 parts of compound enzyme (comprising lipase, amylase and cellulase in a mass ratio of 1:1:1), 40 parts of sodium polyacrylate, 10 parts of active carbon, 5 parts of zeolite powder and 5 parts of bentonite in parts by weight, (3) oxygenation treatment, which is to increase dissolved oxygen in a water body in an aeration mode, accelerate degradation speed of pollutants and improve self-cleaning capability of the water body, (4) aquatic plant planting, which is to plant lotus root and typha aquatic plants, purify the water body and improve water quality through absorption, adsorption and decomposition of plants, (5) biological membrane purification, which is to firmly attach to the bottom or side wall of a river channel by using an anchor device, install the biological membrane on the anchor device, reproduce microorganisms on a carrier and degrade organic pollutants through biological action, and purify the water body, wherein the biological membrane preparation step comprises the steps of (5-1) cleaning the biological membrane carrier made of polylactic acid materials, drying the cleaned biological membrane carrier at 60 ℃, drying time of 8 ℃ and plasma treatment in a carrier treatment area by using plasma treatment equipment, wherein the plasma treatment equipment is used for plasma treatment of the biological membrane, the method comprises the steps of (1) bombarding the surface of a biological film carrier by high-energy particles in oxygen plasma in the treatment process to enable the surface of the biological film carrier to be subjected to plasma modification, wherein the plasma acts on the surface of the biological film carrier to enable the surface of the biological film carrier to be etched and activated to form a roughened surface and an active site, the surface area and the biological attachment point of the biological film carrier are increased, the temperature of plasma treatment is 80 ℃, the plasma treatment time is 15s, a modified biological film carrier is prepared, and the step (5-3) is carried out in a microorganism incubator to inoculate cultured microorganisms onto the modified biological film carrier.

Comparative example 2

The method for purifying and treating the black and odorous water body of the river channel in comparative example 2 comprises the following steps of river channel environment investigation, source control and pollution interception, endogenous treatment and repair and monitoring management.

Comparative example 3

The purification treatment method of the river black and odorous water body in the comparative example 3 comprises the following steps of river channel environment investigation, source control and pollution interception, endogenous treatment, ecological restoration and restoration monitoring management.

The ecological restoration method comprises the steps of (1) restoring the natural purification function of a water body through grass planting ditches and an ecological bank protection mode, (2) carrying out ecological purification, namely adding a biological enzyme preparation into a river water body, wherein the adding amount of the biological enzyme preparation is 5g/m ³ based on the adding amount of each cubic meter of the water body, the biological enzyme preparation comprises 100 parts of compound enzyme (comprising lipase, amylase and cellulase in a mass ratio of 1:1:1), 40 parts of sodium polyacrylate, 10 parts of activated carbon, 5 parts of zeolite powder and 5 parts of bentonite in parts by weight, 3) carrying out oxygenation treatment, namely increasing the dissolved oxygen in the water body in an aeration mode, accelerating the degradation speed of pollutants, improving the self-purification capability of the water body, and 4) planting aquatic plants, namely planting lotus roots and typha aquatic plant in the water body, purifying the water body and improving the water quality through the absorption, adsorption and decomposition effects of the plant.

Comparative example 4

The purification treatment method of the river black and odorous water body in the comparative example 4 comprises the following steps of river channel environment investigation, source control and pollution interception, endogenous treatment, ecological restoration and restoration monitoring management.

Restoring the natural purification function of the water body by a grass planting ditch and an ecological bank protection mode; (2) ecological purification, namely, putting a biological enzyme preparation into a river water body, wherein the putting amount of the biological enzyme preparation is 5g/m ³ according to the putting amount of each cubic meter of water body; the biological enzyme preparation comprises, by weight, 100 parts of a complex enzyme (comprising lipase, amylase and cellulase in a mass ratio of 1:1:1), 40 parts of sodium polyacrylate, 10 parts of activated carbon, 5 parts of zeolite powder and 5 parts of bentonite, (3) oxygenation treatment, which is to increase dissolved oxygen in the water body in an aeration manner, accelerate degradation speed of pollutants and improve self-cleaning capability of the water body, (4) aquatic plant planting, which is to plant lotus roots and typha aquatic plants, purify the water body by absorption, adsorption and decomposition of plants and improve water quality, (5) biological membrane purification, which is to firmly attach to the bottom or side wall of a river channel by using an anchor device, install the biological membrane on the anchor device, and reproduce microorganisms on carriers and degrade organic pollutants by biological action, and purify the water body, wherein the biological membrane preparation step comprises the steps of (5-1) cleaning the biological membrane carriers made of polylactic acid materials, drying the cleaned biological membrane carriers at 60 ℃, the drying temperature of 60 ℃, the biological membrane purification step (5-2) and the biological membrane cultivation step comprises the steps of culturing microorganisms in a biological membrane suspension culture tank, the microorganism is dechlorinated monad) is sprayed on the surface of the biological film carrier, so that the microorganism is attached and inoculated on the surface of the biological film carrier to form a microorganism loading layer, and the biological film is prepared.

Performance test:

1. The organic matter and nutritive salt removing effect is to detect the chemical oxygen demand (COD concentration), nitrogen and phosphorus content and biochemical oxygen demand (BOD concentration) of the water body, measure the pH value change condition, and evaluate the purifying degree of the purifying treatment and restoration methods of the examples and the comparative examples on the black and odorous water body of the river channel. The higher the values of the chemical oxygen demand, the nitrogen and phosphorus content and the biochemical oxygen demand of the water body are, the lower the purification degree of the water body is, and the pH value is in the range of 6.5-7.5, so that the method is more suitable for the growth of aquatic organisms and aquatic plants.

2. The odor, chromaticity and turbidity change of the water body, namely the OU value of the odor concentration of the water body sample is detected, and the specific test result is shown as the odor concentration in table 1. Wherein, the higher the odor concentration value is, the lower the purification degree of the water body is.

3. And (3) Scanning Electron Microscope (SEM) testing, namely adhering a sample on a sample table by adopting conductive adhesive, carrying out surface metal spraying treatment, observing the surface morphology of the sample by adopting a scanning electron microscope (model: merlin of Zeiss, germany), and testing the polylactic acid fiber biomembrane carrier selected in the step (5-1), the enzyme modified biomembrane carrier prepared in the step (5-3) and the Scanning Electron Microscope (SEM) image of the biomembrane prepared in the step (5-4) in the embodiment 1. The corresponding test results are shown in fig. 3-5.

4. IR Spectroscopy test the sample powder was mixed with potassium bromide powder and pressed into tablets, and the IR spectrum information in the 500 cm ^-1～4000 cm^-1 wave number range was examined using a Fourier IR spectrum analyzer (model number TENSOR, bruker, germany). The polylactic acid fiber biomembrane carrier (corresponding to the curve before modification) selected in the step (5-1) and the modified biomembrane carrier (corresponding to the curve after modification) prepared in the step (5-2) of the embodiment 1 of the present application have infrared spectrograms.

TABLE 1

Comparing fig. 3,4 and 5, it can be seen that the surface roughness of the plasma-modified and enzyme-modified surface (fig. 4) is high, and the specific surface area is large, so that more attachment sites can be provided for microorganisms, compared to the surface of the biofilm carrier (fig. 3) which is not treated by plasma. In addition, in the SEM image shown in FIG. 4, white dots represent aggregates of active enzyme molecules, and at this time, a large number of active enzyme molecules are supported on the biofilm carrier, indicating roughened surfaces and active sites formed by plasma modification, which is more favorable for the supporting of active enzyme molecules. Further, the white marked area of fig. 5 corresponds to a large microbial load, and the main reason for this phenomenon is that the introduction of active enzymes increases the affinity between the biofilm carrier and the microorganisms. In FIG. 6, "before modification" corresponds to the polylactic acid fiber biofilm carrier before plasma modification, and "after modification" corresponds to the modified biofilm carrier prepared after plasma modification, and as can be seen from FIG. 6, after plasma treatment of the biofilm carrier, the number of hydroxyl groups on the surface of the modified biofilm carrier is increased, and at this time, more attachment sites capable of fixing active enzymes are formed.

As can be seen from Table 1, the biological film in example 1 was subjected to plasma treatment and enzyme immobilization treatment, so that the biochemical oxygen demand, ammonia nitrogen content, chemical oxygen demand and pH value were all reduced, which indicates that the purification treatment method of example 1 has excellent organic matter and nutrient salt removal effects, and at the same time, the odor concentration of example 1 is lower, which indicates that the purification treatment method of example 1 of the present application is most excellent. The specific biological membrane can effectively adhere microorganisms to the carrier for reproduction, and degrade organic pollutants through biological action, so that the biological activity of the microorganisms is effectively improved, and the treatment effect and the water purification capability of black and odorous water are further improved. The method shows that the modification treatment of the surface of the biological film carrier by combining physical, chemical and biological methods can promote the adhesion and growth of microorganisms on the surface of the carrier, improve the forming speed and stability of the biological film, ensure the forming quality of the biological film and accelerate the forming process of the biological film.

The biofilm in comparative example 1 was not enzyme-loaded and had a poorer degree of purification than that of example 1. In comparative example 2, the ecological restoration step was not performed, and the purification degree was limited. Comparative example 3 does not contain a biofilm purification step, and the purification effect is slightly better than that of comparative example 2, but the purification degree is still limited. The biofilm in comparative example 4 was not plasma treated and enzyme was not immobilized, resulting in limited ecological restoration of water body and inferior purification effect to example 1.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples merely represent a few embodiments of the present application, which facilitate a specific and detailed understanding of the technical solutions of the present application, but are not to be construed as limiting the scope of the application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application. It should be understood that, based on the technical solutions provided by the present application, those skilled in the art may obtain technical solutions through logical analysis, reasoning or limited experiments, which are all within the scope of protection of the appended claims. The scope of the patent of the application should therefore be determined with reference to the appended claims, which are to be construed as in accordance with the doctrines of claim interpretation.

Claims

1. A method for purifying and treating a water body, characterized in that it comprises an ecological restoration step, wherein the ecological restoration comprises a biofilm purification step, wherein the biofilm purification step comprises: fixing the biofilm on the bottom or side wall of a river channel to purify the water body;

The steps of preparing the biofilm include:

The surface of the biofilm carrier is plasma modified by plasma treatment to prepare a modified biofilm carrier; wherein the temperature of the plasma treatment is 80°C to 170°C, the plasma modification includes etching and activation, and the process of the plasma modification at least includes: applying plasma to the surface of the biofilm carrier to etch and activate the surface of the biofilm carrier to form a roughened surface and active sites;

The active enzyme is fixed on the plasma-modified surface of the modified biofilm carrier to achieve enzyme modification, thereby preparing an enzyme-modified biofilm carrier; wherein the active enzyme is fixed in a manner comprising: at least one of physical attachment, coupling connection between the active enzyme and the active site, and intermolecular interaction;

The microorganism suspension is applied to the enzyme-modified surface of the enzyme-modified biofilm carrier, and the affinity between the active enzyme and the microorganism is used to make the microorganism adhere to and inoculate the enzyme-modified surface of the enzyme-modified biofilm carrier to form a microorganism load layer to prepare the biofilm; wherein the microorganism is a microorganism capable of biodegrading organic pollutants in the water body;

Wherein, the material of the biofilm carrier includes one or more of degradable polymers and natural fibers;

The active enzymes include one or more of protease, amylase, laccase, cellulase, hemicellulase, peroxidase, lipoxygenase, phosphatase, urease and glucose oxidase;

The microbial suspension includes microorganisms, and the microorganisms include one or more of the genera Dechloromonas, Gemmatimonas, Steroidobacterium, Saprospira and Comamonas.

2. The method for purifying water according to claim 1, wherein the biofilm preparation step has one or more of the following characteristics:

(1) In the step of plasma-modifying the surface of the biofilm carrier by plasma treatment, the plasma treatment includes oxygen plasma treatment, and the active sites formed include hydroxyl groups;

(2) In the step of plasma modification of the surface of the biofilm carrier using plasma treatment, the plasma treatment time is 8s~15s.

3. The water purification method according to claim 2, characterized in that the biofilm preparation step comprises:

The biofilm carrier is cleaned and dried; wherein the drying temperature is 30°C to 60°C;

The modified biofilm carrier is prepared by plasma modification of the surface of the biofilm carrier by plasma treatment at 80°C to 170°C; wherein the plasma treatment includes oxygen plasma treatment, and the time of the plasma treatment is 8s to 15s; the plasma modification includes etching and activation, and the process of the plasma modification at least includes: applying plasma to the surface of the biofilm carrier to etch and activate the surface of the biofilm carrier to form a roughened surface and active sites, wherein the active sites include hydroxyl groups;

A microbial suspension is coated on the enzyme-modified surface of the enzyme-modified biofilm carrier, and the affinity between the active enzyme and the microorganism is used to make the microorganism adhere to and inoculate on the enzyme-modified surface of the enzyme-modified biofilm carrier to form a microbial load layer to prepare the biofilm; wherein the microorganism is a microorganism capable of biodegrading organic pollutants in the water body.

4. The method for purifying and treating a water body according to any one of claims 1 to 3, characterized in that the step of ecological restoration further comprises one or more of shore restoration, ecological purification, oxygenation treatment and aquatic plant planting steps, and the step of ecological restoration of the water body has one or more of the following characteristics:

(1) The shore restoration method includes planting terrestrial plants;

(2) The oxygenation treatment step includes: increasing dissolved oxygen in the water body by aeration;

(3) The aquatic plant planting includes one or more of lotus root planting and cattail planting.

5. The method for purifying and treating a water body according to claim 4, characterized in that the step of ecological purification comprises: adding a biological enzyme preparation into the water body;

Wherein, the step of adding the biological enzyme preparation to the water body has one or more of the following characteristics:

(1) The amount of the bio-enzyme preparation added is 5 g/m ³ to 8 g/m ³ per cubic meter of water;

(2) In parts by weight, the biological enzyme preparation includes 100 parts of complex enzyme, 40 parts to 60 parts of sodium polyacrylate, 10 parts to 18 parts of activated carbon, 5 parts to 10 parts of zeolite powder, and 5 parts to 10 parts of bentonite.

6. The water purification method according to any one of claims 1 to 3 is characterized in that the water purification method also includes the steps of source control and pollution interception, internal source treatment and restoration monitoring management performed in sequence; wherein the ecological restoration step is performed after the internal source treatment step and before the restoration monitoring management step.

7. The water purification method according to claim 6, characterized in that the endogenous treatment includes one or more of garbage cleaning, flocculation sedimentation and dredging; the endogenous treatment has one or more of the following characteristics:

(1) The garbage cleaning includes one or more of cleaning coastal garbage, cleaning seasonal fallen leaves, cleaning biological remains and cleaning floating objects;

(2) The dredging includes clearing the bottom mud.

8. The method for purifying and treating a water body according to claim 7, characterized in that the step of flocculation and sedimentation comprises: adding a flocculant into the water body;

Wherein, the step of adding a flocculant to the water body has one or more of the following characteristics:

(1) The dosage of the flocculant is 95 g/m ³ to 110 g/m ³ per cubic meter of water;

(2) In parts by weight, the flocculant includes 100 parts of aluminum sulfate, 40 parts to 60 parts of polyferric sulfate, 20 parts to 30 parts of ferric chloride, 20 parts to 30 parts of polyvinyl alcohol, 15 parts to 30 parts of amylase, and 90 parts to 100 parts of activated carbon.

9. The water purification method according to claim 6, characterized in that the water purification method has one or more of the following characteristics:

(1) The steps of source control and pollution interception include: investigating and intercepting the sewage outlets of the water body to control the discharge of sewage into the water body from the source and reduce the pollution load entering the water body;

(2) The steps of restoration monitoring and management include: regular testing and regular inspection and maintenance of the water bodies in several restoration areas and the surrounding environment of the restoration areas.

10. The water purification method according to claim 9, characterized in that before the step of controlling the source of pollution, it also includes the step of environmental investigation;

The water purification method comprises the following steps:

Environmental survey: Divide the water body into several treatment and restoration areas and test the water in each treatment and restoration area, investigate the surrounding environment and pollution source status of the treatment and restoration area, and obtain environmental status data;

Source control and pollution interception: Investigate and intercept the sewage outlets of water bodies to control the discharge of sewage into water bodies from the source and reduce the pollution load entering the water bodies;

Endogenous governance: The endogenous governance includes garbage cleaning, flocculation sedimentation and dredging steps performed in sequence; wherein the garbage cleaning includes one or more of cleaning coastal garbage, cleaning seasonal fallen leaves, cleaning biological remains and cleaning floating objects; the flocculation sedimentation includes: adding flocculants to the water body, and the amount of the flocculants added per cubic meter of water body is 95g/ ^m3 ~110g/ ^m3 ; in terms of weight, the flocculants include 100 parts of aluminum sulfate, 40 parts to 60 parts of polyferric sulfate, 20 parts to 30 parts of ferric chloride, 20 parts to 30 parts of polyvinyl alcohol, 15 parts to 30 parts of amylase, and 90 parts to 100 parts of activated carbon; the dredging includes cleaning the bottom mud;

Ecological restoration: The ecological restoration includes the steps of shore restoration, ecological purification, oxygenation treatment, aquatic plant planting and biofilm purification in sequence; wherein, the shore restoration method includes planting terrestrial plants; the ecological purification includes: adding biological enzyme preparations to the water body, and the amount of the biological enzyme preparations added per cubic meter of water body is 5g/ ^m3 ~8g/ ^m3 ; by weight, the biological enzyme preparations include 100 parts of complex enzymes, 40 parts to 60 parts of sodium polyacrylate, 10 parts to 18 parts of activated carbon, 5 parts to 10 parts of zeolite powder, and 5 parts to 10 parts of bentonite; the oxygenation treatment includes: increasing the dissolved oxygen in the water body by aeration; the aquatic plant planting includes planting one or more of lotus roots and cattail aquatic plants;

Restoration monitoring and management: Regular testing and regular inspection and maintenance of water bodies in several restoration areas and the surrounding environment of the restoration areas.