CN118655198B - Method for improving sensitivity of electrochemical system in detecting heavy metal pollutants and application thereof - Google Patents
Method for improving sensitivity of electrochemical system in detecting heavy metal pollutants and application thereof Download PDFInfo
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Abstract
本申请涉及生物传感器领域，具体公开了提高电化学系统检测重金属污染物灵敏度的方法和用途，该方法包括：（1）构建三电极电化学系统，电化学系统包括对电极、参比电极和工作电极，工作电极表面附着有贝氏不动杆菌BD4；（2）将电解液加入到电化学系统中，将电化学系统置于预设温度下，向电化学系统施加预设的恒电势，记录电化学系统的输出电流；（3）将待测重金属污染物加入到电化学系统中，记录电化学系统的输出电流；（4）计算水质的毒性系数。本申请首次采用贝氏不动杆菌BD4检测重金属污染物，提高了电化学系统检测重金属污染物的灵敏度，实现了微量重金属污染物的检测，使检测下限达到0.05mg/L。
The present application relates to the field of biosensors, and specifically discloses a method and use for improving the sensitivity of an electrochemical system in detecting heavy metal pollutants. The method comprises: (1) constructing a three-electrode electrochemical system, wherein the electrochemical system comprises a counter electrode, a reference electrode and a working electrode, and the surface of the working electrode is attached with Acinetobacter bernini BD4; (2) adding an electrolyte to the electrochemical system, placing the electrochemical system at a preset temperature, applying a preset constant potential to the electrochemical system, and recording the output current of the electrochemical system; (3) adding a heavy metal pollutant to be detected to the electrochemical system, and recording the output current of the electrochemical system; (4) calculating the toxicity coefficient of water quality. The present application uses Acinetobacter bernini BD4 for the first time to detect heavy metal pollutants, thereby improving the sensitivity of the electrochemical system in detecting heavy metal pollutants, realizing the detection of trace heavy metal pollutants, and making the detection limit reach 0.05 mg/L.
Description
Technical Field
The application belongs to the technical field of biosensors, and particularly relates to a method for improving sensitivity of an electrochemical system for detecting heavy metal pollutants and application thereof.
Background
At present, the heavy metal detection in the water environment mainly adopts a physical and chemical analysis method, such as an atomic absorption spectrometry, an X-ray fluorescence spectrometry and the like, and has the characteristics of strong specificity and high sensitivity. However, physicochemical analytical methods generally require expensive instrumentation, complex pretreatment processes, and long detection cycles, and are not suitable for rapid detection in the field. Therefore, the biological method is hopeful to make up for the limitation of physicochemical analysis method in heavy metal detection. Based on the principle that organisms are adapted to the environment, the metabolic activity of the organisms is inhibited when heavy metal pollutants appear in the water body, and the heavy metal pollutant level of the water body is estimated through the change of the metabolism level of the organisms. The biological method for detecting the heavy metal pollutants in the water has the advantages of simple operation, rapid response, low cost and the like. The core of the biological detection method is the detected organism, and common fish, algae, fleas, bacteria and the like, wherein the detection method using the electrochemical active microorganism as the detected organism has remarkable advantages.
However, the technology still has some defects, and mainly has low detection sensitivity, so that trace heavy metal pollutants in water bodies are difficult to detect, and the current water environment quality standard in China cannot be met. In the prior art, the prepared biosensor is subjected to pre-stimulation of toxic substances before on-machine testing, so that the sensor is in a state of being sensitive to heavy metal pollutants before heavy metal testing, and the sensitivity to the heavy metal pollutants is improved. However, the method for pre-stimulating the toxic substances is very complicated in operation, needs to additionally increase the treatment of pre-stimulating the toxic substances, needs to fumbly key parameters such as the concentration of the toxic substances, the stimulation time, the stimulation times and the like used in the pre-stimulation process, needs to monitor the electric signals of sensors, calculate the change condition of the electric signals and the like in the stimulation process in real time, and more importantly, the method for pre-stimulating the toxic substances has limited improvement on the sensitivity of heavy metal pollutants, and can not enable the detection limit of the heavy metal to reach 0.05mg/L through the pre-stimulation treatment.
Disclosure of Invention
The present application aims to solve at least one of the technical problems in the related art to some extent. Therefore, the application aims to provide a method for improving the sensitivity of an electrochemical system for detecting heavy metal pollutants and application thereof. According to the application, the heavy metal sensitive bacteria-acinetobacter bailii BD4 is adopted for detecting the heavy metal pollutants for the first time, so that the sensitivity of an electrochemical system for detecting the heavy metal pollutants is improved, the detection of trace heavy metal pollutants is realized, the lower detection limit reaches 0.05mg/L, and the heavy metal detection has a good linear dose-effect curve relationship in the range of 0.05mg/L to 0.5 mg/L.
In one aspect of the application, a method for increasing the sensitivity of an electrochemical system for detecting heavy metal contaminants is provided. According to an embodiment of the application, the method comprises:
(1) Constructing a three-electrode electrochemical system, wherein the electrochemical system comprises a counter electrode, a reference electrode and a working electrode, and the surface of the working electrode is attached with acinetobacter bailii BD4;
(2) Adding electrolyte into the electrochemical system, placing the electrochemical system at a preset temperature, applying a preset constant potential to the electrochemical system, and recording the output current I 0 of the electrochemical system;
(3) Adding heavy metal pollutants to be detected into the electrochemical system, and recording the output current of the electrochemical system, wherein the output current of the electrochemical system at the t-th preset time is I t;
(4) According to the formula ) The toxicity coefficient IR of the water quality is calculated,
。
According to the method for improving the sensitivity of the electrochemical system for detecting the heavy metal pollutants, disclosed by the embodiment of the application, by constructing the microbial electrochemical system with the heavy metal sensitive bacteria-acinetobacter bailii BD4 as a tested organism, the detection sensitivity of various heavy metals is obviously improved, the detection of trace heavy metal pollutants is realized, the lower detection limit reaches 0.05mg/L, and the method has a good linear dose-effect curve relationship for heavy metal detection within the range of 0.05mg/L to 0.5 mg/L. The application discloses a method for improving the sensitivity of detecting heavy metal pollutants by using heavy metal sensitive bacteria-acinetobacter bailii BD 4. Compared with the electrochemical active microorganism mixed bacteria, the response sensitivity of the acinetobacter bailii BD4 to various heavy metal pollutants is improved by 7.6-12.8 times. Meanwhile, the lower limit of detection of trace heavy metal pollutants by using the acinetobacter bailii BD4 reaches 0.05mg/L, which is improved by about 10 times compared with the prior art.
In addition, the method according to the above embodiment of the present application may further have the following additional technical features:
in some embodiments of the present application, in step (1), sterile carbon is placed in a culture medium, acinetobacter bailii BD4 is inoculated, and the culture is performed for 12 hours to 18 hours, so that the acinetobacter bailii BD4 is attached to the surface of carbon cloth and forms a biological film, and the carbon cloth attached with the biological film is used as a working electrode of the electrochemical system.
In some embodiments of the application, in step (2), the electrolyte comprises a carbon source of 5-20 mmol/L, naCl of 5.80-5.85 g/L, KCl of 0.12-0.13 g/L, NH 4 Cl of 0.30-0.31 g/L, naH 2PO4•2H2 O of 6.05-6.10 g/L, na 2HPO4•12H2 O of 21.80-21.85 g/L, wherein the carbon source comprises at least one of acetate, propionate, butyrate, glucose, sucrose and soluble starch.
In some embodiments of the present application, in step (2), the preset temperature is set to be T, and the electrochemical system is within t±1 ℃.
In some embodiments of the present application, the preset temperature T is 25 ℃ to 30 ℃.
In some embodiments of the present application, in the step (2), the preset constant potential is +0.2v to +0.5v.
In some embodiments of the application, in step (3), the heavy metal contaminant to be tested comprises at least one of Zn 2+、Tl+、Ni2+、Cu2+、Cd2+、Hg2+、As3+、Pb2+.
In some embodiments of the present application, in the step (3), the concentration of the heavy metal contaminant to be detected in the electrochemical system is 0.05mg/L to 0.5mg/L.
In some embodiments of the application, in step (4), the water quality is judged to be toxic when IR is greater than or equal to 5%, and is judged to be non-toxic when IR is greater than or equal to 0 and less than or equal to 5%.
In a second aspect of the present application, the present application provides a use of the method for improving sensitivity of an electrochemical system for detecting heavy metal pollutants in water toxicity detection according to the above embodiment. Therefore, high-sensitivity, rapid and low-cost monitoring of low-concentration heavy metals in the water body can be realized.
Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.
Drawings
The foregoing and/or additional aspects and advantages of the application will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic flow chart of a method for improving the sensitivity of an electrochemical system for detecting heavy metal pollutants according to an embodiment of the application.
FIG. 2 is a graph showing comparison of inhibition ratios of 8 heavy metal pollutants detected by using Acinetobacter belleville BD4 in example 1, wherein a-b in FIG. 2 are schematic diagrams of I t -t for detecting 8 heavy metal pollutants of 0.3mg/L, and c-d in FIG. 2 are graphs showing comparison of inhibition ratios of 8 heavy metal pollutants detected by mixing Acinetobacter belleville BD4 with electrochemically active microorganisms of 0.3 mg/L.
FIG. 3 is a graph showing the results of detecting 4 trace heavy metal pollutants by using Acinetobacter bellianum BD4 in example 1 of the present application, wherein FIG. 3a is a schematic diagram of I t -t of detecting 4 heavy metal pollutants of 0.05mg/L by using Acinetobacter bellianum BD4, and FIG. 3b is a bar chart of detecting 4 heavy metal pollutants of 0.05mg/L by using Acinetobacter bellianum BD 4.
Fig. 4 is a schematic diagram of a result of linear fitting of the detection of Cd 2+、Pb2+ heavy metal pollutants by using acinetobacter bailii BD4 and the detection of Cd 2+、Pb2+ heavy metal pollutants according to example 1 of the present application, in which fig. 4a is a schematic diagram of I t -t of the detection of Cd 2+ at different concentrations by using acinetobacter bailii BD4, fig. 4b is a schematic diagram of I t -t of the detection of Pb 2+ at different concentrations by using acinetobacter bailii BD4, fig. 4c is a schematic diagram of a result of linear fitting of the detection of Cd 2+ by using acinetobacter bailii BD4, and fig. 4d is a schematic diagram of a result of linear fitting of the detection of Pb 2+ by using acinetobacter bailii BD 4.
Detailed Description
Embodiments of the present application are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present application and should not be construed as limiting the application.
The present application has been made based on the following problems:
in the related technology, natural electrochemical active microorganism mixed bacteria are adopted to detect heavy metal pollutants in water, but the detection sensitivity of the mixed bacteria is low, so that trace heavy metal pollutants in water are difficult to detect, and the existing water environment quality standard cannot be met.
The inventors have found that the use of electrochemically active microorganisms that are sensitive to heavy metal contaminants is expected to increase the sensitivity of detecting heavy metal contaminants. This is because the detection of heavy metals by electrochemically active microorganisms is based on the stress of heavy metal contaminants on bacteria, whereas different kinds of microorganisms have a difference in resistance to heavy metal contaminants. Therefore, the detection sensitivity can be improved by utilizing the electrochemical active microorganisms sensitive to the heavy metal pollutants, so that the trace heavy metal pollutants in the water body can be detected more effectively.
In view of this, in one aspect of the present application, a method for improving the sensitivity of an electrochemical system for detecting heavy metal contaminants is provided. According to the embodiment of the application, the method comprises (1) constructing a three-electrode electrochemical system, wherein the electrochemical system comprises a counter electrode, a reference electrode and a working electrode, the surface of the working electrode is attached with the acinetobacter bailii BD4, (2) adding electrolyte into the electrochemical system, placing the electrochemical system at a preset temperature, applying a preset constant potential to the electrochemical system, recording the output current I 0 of the electrochemical system, (3) adding heavy metal pollutants to be detected into the electrochemical system, recording the output current of the electrochemical system, wherein the output current of the electrochemical system at the preset time t is I t, and (4) according to the formula [%) The toxicity coefficient IR of the water quality is calculated,。
According to the method for improving the sensitivity of the electrochemical system for detecting the heavy metal pollutants, disclosed by the embodiment of the application, the detection sensitivity of various heavy metals can be obviously improved by constructing the microbial electrochemical system with the heavy metal sensitive bacteria-acinetobacter bailii BD4 as a tested organism, so that the detection of trace heavy metal pollutants is realized, the lower limit of detection reaches 0.05mg/L, and the method has a good linear dose-effect curve relationship for heavy metal detection within the range of 0.05mg/L to 0.5 mg/L.
The application discloses a method for improving the sensitivity of detecting heavy metal pollutants by using heavy metal sensitive bacteria-acinetobacter bailii BD 4. Compared with the electrochemical active microorganism mixed bacteria, the response sensitivity of the acinetobacter bailii BD4 to various heavy metal pollutants is improved by 7.6-12.8 times. Meanwhile, the lower limit of detection of trace heavy metal pollutants by using the acinetobacter bailii BD4 reaches 0.05 mg/L, which is improved by about 10 times compared with the prior art.
The method for improving the sensitivity of the electrochemical system for detecting the heavy metal pollutants is described in detail below:
Specifically, referring to fig. 1, the method for improving the sensitivity of the electrochemical system for detecting heavy metal pollutants includes the following steps:
s100, constructing a three-electrode electrochemical system;
In this step, a three-electrode electrochemical system is constructed, which includes a counter electrode, a reference electrode, and a working electrode to which acinetobacter bailii BD4 is attached. The above acinetobacter bailii BD4 was purchased from the american standard organism collection under accession number ATCC 33304.
According to some embodiments of the present application, a three-electrode electrochemical system is constructed, the working volume of which may be 50mL, the counter electrode may be a 1cm by 1cm platinum sheet electrode, and the reference electrode may be an Ag/AgCl electrode (0.205V vs. standard hydrogen electrode).
According to still other embodiments of the present application, in step S100, a sterile carbon cloth (e.g., 2cm×2cm sterile carbon cloth) is placed in a medium (e.g., luria-Bertani medium), acinetobacter behenryi BD4 is inoculated, and shaking culture is performed for 12 hours to 18 hours, so that acinetobacter behenryi BD4 adheres to the surface of the carbon cloth and forms a biofilm, and the carbon cloth adhered with the biofilm is used as a working electrode of an electrochemical system.
S200, adding electrolyte into an electrochemical system, placing the electrochemical system at a preset temperature, applying a preset constant potential to the electrochemical system, and recording the output current I 0 of the electrochemical system;
in this step, a certain amount of electrolyte (for example, 50mL of electrolyte) is added to the electrochemical system, the electrochemical system is placed at a preset temperature, a preset constant potential is applied to the electrochemical system by using a potentiostat to provide a voltage environment, an electrical signal is output, and a stable current I 0 output by the electrochemical system is recorded.
According to further embodiments of the present application, in step S200, the electrolyte comprises a carbon source of 5mmol/L to 20mmol/L, naCl of 5.80g/L to 5.85g/L, KCl of 0.12g/L to 0.13g/L, NH 4 Cl of 0.30g/L to 0.31g/L, naH 2PO4•2H2 O of 6.05g/L to 6.10g/L, na 2HPO4•12H2 O of 21.80g/L to 21.85g/L, and the carbon source comprises at least one of acetate (e.g., sodium acetate), propionate (e.g., sodium propionate), butyrate (e.g., sodium butyrate), glucose, sucrose, and soluble starch. The electrolyte is prepared by using water as a solvent.
According to still other embodiments of the present application, in step S200, the preset temperature is set to be T, and the electrochemical system is within t±1 ℃, i.e., the electrochemical system needs to be in a constant temperature environment.
According to further embodiments of the present application, the preset temperature T is 25 ℃ to 30 ℃, e.g. the preset temperature T may be 25 ℃, 26 ℃, 27 ℃, 28 ℃, 29 ℃, 30 ℃ or the like. When the preset temperature T is 25 ℃, the electrochemical system is within 25±1 ℃.
According to still other embodiments of the present application, in step S200, the preset constant potential is +0.2v to +0.5v, for example +0.2v, +0.3v, +0.4v, +0.5v, or the like.
S300, adding heavy metal pollutants to be detected into an electrochemical system, and recording output current of the electrochemical system;
In the step, heavy metal pollutants to be detected are added into an electrochemical system, the output current of the electrochemical system is recorded, and the output current of the electrochemical system at the t-th preset time is I t.
According to still other embodiments of the present application, the heavy metal contaminant to be detected includes at least one of Zn2+、Tl+、Ni2+、Cu2+、Cd2 +、Hg2+、As3+、Pb2+, the sensitivity of the response of the acinetobacter besseyi BD4 to the heavy metal contaminant is higher, and compared with the electrochemical active microorganism mixed bacteria, the sensitivity of the response of the acinetobacter besseyi BD4 to the heavy metal contaminant is improved by 7.6 to 12.8 times.
According to still other embodiments of the present application, in step S300, the concentration of the heavy metal contaminant to be detected in the electrochemical system is 0.05mg/L to 0.5mg/L, for example, may be 0.05mg/L, 0.1mg/L, 0.2mg/L, 0.3mg/L, 0.4mg/L, 0.5mg/L, etc. According to the application, by constructing a microbial electrochemical system with heavy metal sensitive bacteria-acinetobacter bailii BD4 as a tested organism, the detection sensitivity of various heavy metals is obviously improved, the detection of trace heavy metal pollutants is realized, the detection lower limit reaches 0.05mg/L, and the detection of heavy metals has a good linear dose-effect curve relationship within the range of 0.05 mg/L-0.5 mg/L.
S400, calculating the toxicity coefficient IR of the water quality according to a formula.
In this step, according to the formula [ ]) The toxicity coefficient IR of the water quality is calculated,
。
In the embodiment of the application, when IR is more than or equal to 5%, the water quality is judged to be toxic, and when IR is more than or equal to 0 and less than 5%, the water quality is judged to be non-toxic.
In a second aspect of the present application, the present application provides a use of the method for improving sensitivity of an electrochemical system for detecting heavy metal pollutants in water toxicity detection according to the above embodiment. Therefore, high-sensitivity, rapid and low-cost monitoring of low-concentration heavy metals in the water body can be realized.
The following detailed description of embodiments of the application is provided for the purpose of illustration only and is not to be construed as limiting the application. In addition, all reagents employed in the examples below are commercially available or may be synthesized according to methods herein or known, and are readily available to those skilled in the art for reaction conditions not listed, if not explicitly stated.
Example 1
An electrochemical system consisting of a working electrode, a counter electrode and a reference electrode was constructed. The working electrode, the counter electrode and the reference electrode were respectively selected from a 2cm×2cm carbon cloth (WOS 1009, carbon technologies Co., ltd.), a 1cm×1cm platinum sheet (Pt 210, tianjin ida Heng Cheng technologies Co., ltd.) and a standard Ag/AgCl electrode (RO 303, tianjin ida Heng technologies Co., ltd.; 0.205V vs. standard hydrogen electrode), and all the parts except the reference electrode were sterilized by high temperature and high pressure, and the reference electrode was immersed in 75% alcohol overnight. The sterilized carbon cloth electrode was placed in Luria-Bertani (A507002-0250) medium, and inoculated with Acinetobacter bailii BD4 (purchased from the American standard organism collection center, accession No. ATCC 33304) and cultured overnight with shaking at 25℃for 16 hours, so that microorganisms were attached to the surface of the carbon cloth and formed a biofilm, and the biofilm-attached carbon cloth was used as an electrochemical system working electrode. After each fitting is ready to be assembled, it is completed in a clean bench (SW-CJ-1F, sujingtai).
50ML of sterile electrolyte was added to the electrochemical system, including 5.85g/L NaCl, 0.13g/L KCl, 0.31g/L NH 4 Cl, 6.08g/L NaH 2PO4•2H2 O, 21.83g/L Na 2HPO4•12H2 O, 10mmol/L glucose. The electrochemical system was placed in a 25 ℃ constant temperature incubator (HPS-500, halbine eastern electronics development limited) and a multi-channel potentiostat (CHI 1030C, shanghai cinnabar instruments limited) was used to apply a +0.5v potential (vs. reference electrode), record the output electrical signal of the electrochemical system 20min before toxicity test, and set the stable current parameter output by the electrochemical system to I 0. Then respectively adding 8 heavy metal pollutants to be detected (Zn 2+、Tl+、Ni2+、Cu2+、Cd2+、Hg2+、As3+、Pb2+ respectively) into each parallel electrochemical system independently, respectively adding the same heavy metal pollutants with different concentrations into each parallel electrochemical system independently, continuously recording the output current of the electrochemical system for 20min, and recording the current of the electrochemical system at the 20 th min as I t. Through the formula [ (]) IR was calculated and the degree of inhibition of heavy metal contaminants on the output current of Acinetobacter belli BD4 was evaluated.
Comparative example 1
This comparative example is essentially identical to the process of example 1, except that:
The electrochemical active microorganism mixed bacteria are adopted to replace the acinetobacter besii BD4.
FIG. 2 is a graph showing comparison of inhibition ratios of 8 heavy metal pollutants detected by using Acinetobacter belleville BD4 in example 1, wherein a-b in FIG. 2 are schematic diagrams of I t -t for detecting 8 heavy metal pollutants of 0.3mg/L, and c-d in FIG. 2 are graphs showing comparison of inhibition ratios of 8 heavy metal pollutants detected by mixing Acinetobacter belleville BD4 with electrochemically active microorganisms of 0.3 mg/L.
As can be seen from a-b in fig. 2, the output current of the electrochemical system is basically stable within 20min before the toxicity test is prepared, specifically, the fluctuation of the output current of the electrochemical system does not exceed 2% within the first 20min, which indicates that the acinetobacter belli BD4 can stably output current under the normal condition of the water quality of the water body. At 20min, after 0.3mg/L of each heavy metal pollutant is independently added into each parallel electrochemical system, the output current of the acinetobacter belleville BD4 is obviously reduced, which indicates that heavy metal pollutant detection can be effectively realized by using the acinetobacter belleville BD 4.
As can be seen from c-d in FIG. 2, when the acinetobacter besseyi BD4 is exposed to the environment of 0.3mg/L Zn 2+、Tl+、Ni2+、Cu2 + for 20min (namely, 40min in FIG. 2 a), the current inhibition rates are 18.1%, 47.4%, 21.2% and 24.3%, respectively, and the mixed bacteria after the same heavy metal pollutants are exposed for 20min have the electrical signal inhibition rates of only 1.8%, 3.8%, 2.8% and 3.1% (as shown in FIG. 2 c), and similarly, when the acinetobacter besseyi BD4 is exposed to the environment of 0.3mg/L Cd 2+、Hg2+、As3+、Pb2+ for 20min (namely, 40min in FIG. 2 b), the current inhibition rates are 36.7%, 53.1%, 41.5% and 30.1%, respectively, and the mixed bacteria after the same heavy metal pollutants are exposed for 20min have the electrical signal inhibition rates of 3.6%, 4.7%, 3.7% and 3.1% (as shown in FIG. 2 d).
FIG. 3 is a graph showing the results of detecting 4 trace heavy metal pollutants by using Acinetobacter bellianum BD4 in example 1 of the present application, wherein FIG. 3a is a schematic diagram of I t -t of detecting 4 heavy metal pollutants of 0.05mg/L by using Acinetobacter bellianum BD4, and FIG. 3b is a bar chart of detecting 4 heavy metal pollutants of 0.05mg/L by using Acinetobacter bellianum BD 4.
As can be seen from FIG. 3b, the current inhibition rates were 7.6%, 6.4%, 10.1% and 8.6%, respectively, when the A.belleville BD4 was exposed to the 0.05mg/L Cd 2+、Pb2+、Hg2+、Tl+ environment for 20min (i.e., at 40min in FIG. 3 a).
Fig. 4 is a schematic diagram of a result of linear fitting of the detection of Cd 2+、Pb2+ heavy metal pollutants by using acinetobacter bailii BD4 in embodiment 1 of the present application, wherein fig. 4a is a schematic diagram of I t -t of the detection of Cd 2+ at different concentrations by using acinetobacter bailii BD4, fig. 4b is a schematic diagram of I t -t of the detection of Pb 2+ at different concentrations by using acinetobacter bailii BD4, fig. 4c is a schematic diagram of a result of linear fitting of the detection of Cd 2+ by using acinetobacter bailii BD4, and fig. 4d is a schematic diagram of a result of linear fitting of the detection of Pb 2+ by using acinetobacter bailii BD 4.
As can be seen from FIG. 4a, the current inhibition rate reached 7.6% after the B.belleville BD4 was exposed to the 0.05mg/L Cd 2+ environment for 20min (i.e., at 40min of FIG. 4 a), and the current inhibition rates were 15.7%, 36.7%, 55.3% when the Cd 2+ concentration was increased to 0.1 mg/L, 0.3 mg/L, and 0.5 mg/L, respectively, and the sensitivity for detecting Cd 2+ contaminant by using the B.belleville BD4 was calculated to be 104.2% (mg/L) -1 (as shown in FIG. 4 c). Similarly, it can be seen from FIG. 4b that the current inhibition rate reached 6.4% after the B.belleville BD4 was exposed to the Pb 2+ environment at 0.05mg/L for 20min (i.e., at 40min in FIG. 4 b), and that the current inhibition rates were 15.5%, 30.1% and 44.2% when the Pb 2+ concentration was increased to 0.1 mg/L, 0.3 mg/L and 0.5 mg/L, respectively. The sensitivity of detecting Pb 2+ contaminants using A.belleville BD4 was calculated to be 80.0% (mg/L) -1 (as shown in FIG. 4 d).
The results fully show that the detection sensitivity of various heavy metals can be remarkably improved by utilizing the acinetobacter bailii BD4, compared with the mixed bacteria of electrochemically active microorganisms, the inhibition rate is respectively improved by 7.6-12.8 times, meanwhile, the detection of trace heavy metal pollutants can be realized by utilizing the acinetobacter bailii, the detection lower limit reaches 0.05mg/L, and the method has a good linear dose-effect curve relationship for heavy metal detection within the range of 0.05 mg/L-0.5 mg/L. Therefore, the method can be suitable for detecting heavy metal pollutants in water, and overcomes the defects of the existing electrochemical active microorganism detection technology in heavy metal detection application.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
While embodiments of the present application have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the application, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the application.
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