CN118620727B - Preimplantation genetic diagnosis embryo biopsy device - Google Patents
Preimplantation genetic diagnosis embryo biopsy device Download PDFInfo
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Abstract
The invention discloses a genetic diagnosis embryo biopsy device before embryo implantation, which relates to the technical field of medical equipment and has the technical scheme that the embryo biopsy device comprises a base, an embryo placement table and a sample placement table, wherein a first support column and a second support column are fixedly arranged on the top surface of the base, the embryo placement table comprises a base plate, a heating plate and a heat conducting plate, the base plate is of a cylindrical groove structure with an opening at the top, the heating plate and the heat conducting plate are sequentially embedded into the base plate, the base plate is rotatably connected with the first support column, a plurality of first placement grooves are formed in the top surface of the heat conducting plate, the sample placement table comprises a placement table which is rotatably connected with the second support column, a plurality of second placement grooves are formed in the top surface of the placement table, and the base plate is movably connected with the placement table. The invention can accurately correlate and sample, can directly detect PGS after sampling, accurately marks the healthy and normal embryo culture dish clearly after the detection is finished through the obvious marking structure, thereby rapidly identifying normal embryo and improving embryo transfer success rate.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a genetic diagnosis embryo biopsy device before embryo implantation.
Background
The genetic screening before embryo implantation (Preimplantation GENETIC SCREENING, PGS 1) refers to the detection of chromosome number and structural abnormality of early embryo before embryo implantation, and the early prenatal screening method for analyzing whether embryo has genetic material abnormality by detecting chromosome structure and number of embryo 23 at one time so as to select normal embryo to implant into uterus, so as to obtain normal pregnancy, improve clinical pregnancy rate of patients and reduce multiple-embryo well-being.
Patent CN113322185A discloses a genetic diagnosis embryo biopsy device before embryo implantation, comprising an incubation table, a transparent cover and a rotary seat, wherein a plurality of incubation stations are arranged on the incubation table, each incubation station is provided with an incubation groove suitable for placing a culture dish and a sampling groove suitable for inserting a sampling tube, the transparent cover is positioned above the incubation table, an opening suitable for exposing one incubation station is arranged on the transparent cover, and the rotary seat is arranged at the bottom of the incubation table and is used for driving the incubation table to rotate relative to the transparent cover so as to selectively switch one incubation station into the opening. The embryo biopsy device for genetic diagnosis before embryo implantation provided by the embodiment of the invention can be used for biopsy sampling, ensures the association between a sample and an embryo in a culture dish, and reduces the possible risk caused by human misoperation.
The existing device is through setting up the cultivation station of correlation to set up the opening that only exposes a cultivation station, in order to ensure the relevance of embryo in sample and the culture dish, and such device is guaranteed the relevance of embryo in sample and the culture dish when taking out, but under the general circumstances, the sample is taken out the back and is directly just detected PGS, and when this device need carry out PGS to the sample and detect, still need take out the sampling tube, not only convenient inadequately, after detecting moreover, still there is the risk of matching error.
Disclosure of Invention
The invention aims to solve the problems, and provides a genetic diagnosis embryo biopsy device before embryo implantation, which is characterized in that an incubation table and a sampling table which are mutually related but independent are designed, PGS detection can be directly carried out after sampling, a remarkable labeling structure is added, a healthy and normal embryo culture dish can be clearly labeled after PGS detection is finished, medical staff can rapidly identify normal embryos, and the embryo transfer success rate is greatly improved.
The technical aim of the embryo biopsy device for genetic diagnosis before embryo implantation is achieved through the following technical scheme that the embryo biopsy device comprises a base, an embryo placement table and a sample placement table, wherein a first support column and a second support column are fixedly arranged on the top surface of the base, the embryo placement table comprises a base plate, a heating plate and a heat conducting plate, the base plate is of a cylindrical groove structure with an opening at the top, the heating plate and the heat conducting plate are sequentially embedded into the base plate, the base plate is rotatably connected with the first support column, a plurality of first placement grooves are formed in the top surface of the heat conducting plate, the sample placement table comprises a placement table, the placement table is rotatably connected with the second support column, a plurality of second placement grooves are formed in the top surface of the placement table, and the base plate is movably connected with the placement table.
Through the technical scheme, the embryo culture device comprises a fixing device, a plurality of sample culture dishes, a rotating chassis, a rotating base plate and a detecting instrument, wherein the fixing device is used for fixing the object placing table near a detecting instrument, for example, at a position which is convenient for a microscope to observe, then embryo culture dishes which need to be detected by a PGS (pulse-width modulation) are respectively placed in a plurality of first object placing grooves, a heating plate can ensure proper temperature required by embryo culture, then the plurality of sample culture dishes are respectively placed in a plurality of second object placing grooves, the rotating chassis is used for driving the object placing table to rotate, when one of the first object placing grooves and the second object placing groove are close, the rotating is stopped, embryo sampling in the embryo culture dishes is placed in the sample culture dishes, after all embryos are sampled, the detecting instrument is used for sequentially and directly detecting each sample culture dish, after detection, embryos corresponding to normal samples are detected are transplanted, the whole process is unnecessary to transfer the sample culture dishes, the operation is convenient, and the risk of embryo matching error after detection can be effectively reduced.
The heat conducting plate is further provided with a plurality of first storage grooves and a plurality of second storage grooves, the first storage grooves and the second storage grooves are equal in number and are respectively arranged at equal intervals along the circumferential directions of the heat conducting plate and the storage table, and the first storage grooves and the second storage grooves are respectively provided with mutually symmetrical notches.
By adopting the technical scheme, the first storage grooves and the second storage grooves with the same quantity are arranged, so that one-to-one correspondence between the embryo culture dish and the sample culture dish can be ensured when sampling is performed, and the first storage grooves and the second storage grooves are provided with mutually symmetrical gaps, so that the culture dish can be conveniently placed and taken out.
The heat conducting plate is further provided with a plurality of groups of signal lamp groups corresponding to the first storage grooves respectively, each group of signal lamp groups comprises two signal lamps with different colors of light, the top surface of the storage table is provided with a plurality of groups of switch groups corresponding to the second storage grooves respectively, each switch group comprises two switches, the switch groups are connected with the signal lamp groups in a one-to-one correspondence manner through electric signals, and two switches are connected with the two signal lamps in the correspondingly connected switch groups in a one-to-one correspondence manner.
By adopting the technical scheme, the normal embryo and the abnormal embryo are marked by the signal lamp group, so that the identification of doctors is facilitated, and the error recording is avoided; the method comprises the steps of after detecting samples in a sample culture dish, if the samples are normal, pressing a normal detection switch for representing the samples in a switch group according to detection results, wherein a signal lamp for representing the normal embryo in a corresponding signal lamp group is lightened, the embryo culture dish is marked as normal, otherwise, pressing a normal detection switch for representing the samples in the switch group, the signal lamp for representing the abnormal embryo is lightened, the embryo culture dish is marked as abnormal, after all the samples are detected, the embryo in the embryo culture dish marked as normal is selected for transplantation, and the remaining normal embryo can be kept in refrigeration.
The invention is further characterized in that a limit column is arranged in the chassis, a bulge is arranged on the side face of the limit column, and limit holes which follow the limit column are formed in the bottoms of the heat conducting fin and the heat conducting plate.
Through adopting above-mentioned technical scheme, the dismantlement and the installation of each part of the device of being convenient for play the location effect to first thing groove of putting.
The invention is further characterized in that a first sleeve is fixedly arranged on the bottom surface of the chassis, the first sleeve is sleeved on the top of the first support column, and the first sleeve is fixedly connected with a first toothed ring.
The invention is further characterized in that the first support column is fixedly connected with a first support plate close to the first sleeve, the first support plate is rotatably connected with a first gear, the first gear is meshed with the first toothed ring, the first gear shaft is connected with a motor, and the motor is fixed on the top surface of the base.
The invention is further characterized in that the bottom of the object placing table is fixedly connected with a second sleeve, the second sleeve is rotatably sleeved on the top of the second support column, and the second sleeve is fixedly connected with a second toothed ring.
The invention is further characterized in that the second support column is fixedly connected with a second support plate, the second support plate is rotatably connected with a second gear, and the second gear is meshed with the first gear and the second toothed ring.
The invention further provides that the first toothed ring and the second toothed ring are identical in structure and size, and the first gear and the second gear are identical in structure and size.
Through adopting above-mentioned technical scheme, the starter motor drives first gear rotation to first ring gear of transmission, second gear, second ring gear are rotatory, then drive chassis and put the thing platform rotatory, under the effect of spacing post, and the heat conduction board is rotatory, because first ring gear and second ring gear structure and size are identical, first gear and second gear structure and size are identical, therefore a plurality of first thing grooves of putting and a plurality of second put thing groove homoenergetic rotation at the same speed and in proper order one-to-one.
The invention further provides that a plurality of L-shaped optical coupling baffle plates are arranged on the bottom surface of the chassis at equal intervals along the circumferential direction, the number of the optical coupling baffle plates is the same as that of the first storage grooves, the positions of the optical coupling baffle plates are in one-to-one correspondence, the first support columns are fixedly connected with third support plates, the third support plates are fixedly connected with photoelectric switches, the optical coupling baffle plates sequentially circulate through the photoelectric switches, and the photoelectric switches are in telecommunication connection with the motor.
Through adopting above-mentioned technical scheme, when a plurality of opto-coupler separation blades pass through photoelectric switch in proper order, can promote motor stop operation, because first ring gear, first gear, second gear and second ring gear mesh in proper order, consequently the heat-conducting plate can synchronous rotation and stop with putting the thing platform, and at every turn stops, has embryo culture dish and sample culture dish that correlates each other to be close to each other for the sample, guarantee the relevance of sample after the sample, avoid the matching error of testing result.
In summary, the invention has the following beneficial effects:
The invention drives the first gear to rotate and drive the first toothed ring, the second gear and the second toothed ring to rotate, then drives the heat-conducting plate and the object placing table to rotate, and because the structures and the sizes of the first toothed ring and the second toothed ring are completely the same, the structures and the sizes of the first gear and the second gear are completely the same, the first object placing grooves and the second object placing grooves can rotate at the same speed and sequentially correspond to each other one by one, and by arranging the photoelectric switch and the optical coupling baffle corresponding to the first object placing grooves, when the optical coupling baffle sequentially passes through the photoelectric switch, the motor can be driven to stop running, and because the first toothed ring, the first gear, the second gear and the second toothed ring are sequentially meshed, the heat-conducting plate and the object placing table can synchronously rotate and stop, and each stop, the embryo culture dish and the sample culture dish which are mutually related are mutually close to each other for sampling, the relevance of the samples after sampling is ensured, and the matching error of detection results is avoided.
According to the invention, the object placing table is close to the side of the detecting instrument, for example, the position convenient for microscope observation, after all embryos are sampled, each sample culture dish is directly detected by the detecting instrument in sequence, after the detection is finished, the embryo corresponding to the normal sample is transplanted, the sample culture dishes are not required to be transferred for many times in the whole process, the operation is convenient, and the risk of embryo matching errors after the detection can be effectively reduced.
The invention uses the signal lamp group to distinguish and mark normal embryo and abnormal embryo, which is convenient for doctor to identify, to avoid error, and the specific operation is that after detecting the sample in one sample culture dish, according to the detection result, if it is normal, the switch group is pressed to detect normal switch, the signal lamp in the corresponding signal lamp group is lightened, the embryo culture dish is marked as normal, otherwise, the switch group is pressed to detect abnormal signal lamp, the embryo culture dish is lightened, the embryo culture dish is marked as abnormal, when all samples are detected, the embryo in the embryo culture dish marked as normal is selected for transplantation, the rest normal embryo can be kept in refrigeration, the marking method is simple and quick, and is very convenient for doctor to identify, greatly improves embryo transplantation success rate.
Drawings
FIG. 1 is a block diagram (top view) of an apparatus in an embodiment of the invention;
FIG. 2 is a block diagram (bottom view) of an apparatus in an embodiment of the invention;
FIG. 3 is an exploded view of an apparatus in an embodiment of the invention;
FIG. 4 is a bottom view of a chassis in an embodiment of the present invention;
FIG. 5 is a diagram of the bottom of the table in an embodiment of the invention;
Fig. 6 is a bottom structural view of a heat conductive plate in an embodiment of the present invention.
In the figure, 1, a base; 2, embryo placement table, 21, chassis, 22, heating plate, 23, heat conducting plate, 24, first placing groove, 211, limit column, 212, first sleeve, 213, first toothed ring, 25, limit hole, 3, sample placement table, 31, placing table, 311, second sleeve, 312, second toothed ring, 32, second placing groove, 4, first support column, 41, first support plate, 42, first gear, 43, motor, 5, second support column, 51, second support plate, 52, second gear, 6, notch, 7, signal lamp group, 8, switch group, 9, optical coupling baffle, 10, third support plate, 11, photoelectric switch, 12, embryo culture dish, 13, sample culture dish.
Detailed Description
In order that those skilled in the art will better understand the present invention, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings, wherein it is to be understood that the illustrated embodiments are merely exemplary of some, but not all, of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other. The present invention will be described in detail with reference to examples.
Examples:
As shown in fig. 1 to 6, the embryo biopsy device for genetic diagnosis before embryo implantation comprises a base 1, an embryo placing table 2 and a sample placing table 3, wherein a first support column 4 and a second support column 5 are arranged on the top surface of the base 1, the embryo placing table 2 comprises a chassis 21, a heating plate 22 and a heat conducting plate 23, the chassis 21 is of a cylindrical groove structure with an opening at the top, a limit column 211 is arranged in the chassis 21, protrusions are arranged on the side surfaces of the limit column 211, limit holes 25 which are formed along with the limit column 211 are arranged at the bottoms of the heat conducting plate and the heat conducting plate 23, and the heating plate 22 and the heat conducting plate 23 are sequentially embedded into the chassis 21 through the limit column 211;
The first sleeve 212 is installed on the bottom surface of the chassis 21, the first sleeve 212 is sleeved on the top of the first support column 4, and the first sleeve 212 is fixedly connected with the first toothed ring 213. The first support column 4 is fixedly connected with a first support plate 41 close to the first sleeve 212, the first support plate 41 is rotatably connected with a first gear 42, the first gear 42 is meshed with the first gear ring 213, the first gear 42 is connected with a motor 43 in an axial mode, and the motor 43 is fixed on the top surface of the base 1.
The sample placing table 3 comprises a placing table 31, a second sleeve 311 is fixedly connected to the bottom of the placing table 31, the second sleeve 311 is rotatably sleeved on the top of the second support column 5, and a second toothed ring 312 is fixedly connected to the second sleeve 311. The second support column 5 is fixedly connected with a second support plate 51, the second support plate 51 is rotatably connected with a second gear 52, and the second gear 52 is meshed with the first gear 42 and the second toothed ring 312. The first gear ring 213 and the second gear ring 312 are identical in structure and size, and the first gear 42 and the second gear 52 are identical in structure and size.
The motor 43 is started to drive the first gear 42 to rotate and drive the first toothed ring 213, the second gear 52 and the second toothed ring 312 to rotate, then drive the chassis 21 and the object placing table 31 to rotate, and under the action of the limiting column 211, the heat conducting plate 23 rotates, and because the structures and the sizes of the first toothed ring 213 and the second toothed ring 312 are identical, the structures and the sizes of the first gear 42 and the second gear 52 are identical, and therefore the first object placing grooves 24 and the second object placing grooves 32 can rotate at the same speed and correspond to each other in sequence.
The bottom surface of the chassis 21 is provided with a plurality of L-shaped optocoupler baffles 9 at equal intervals along the circumferential direction, the number of the optocoupler baffles 9 is the same as that of the first storage grooves 24, the positions of the optocoupler baffles are in one-to-one correspondence, the first support columns 4 are fixedly connected with third support plates 10, the third support plates 10 are fixedly connected with photoelectric switches 11, the optocoupler baffles 9 sequentially circulate through the photoelectric switches 11, and the photoelectric switches 11 are in telecommunication connection with a motor 43. When the plurality of optocoupler baffles 9 sequentially pass through the optoelectronic switch 11, the motor 43 is caused to stop running, and the first toothed ring 213, the first gear 42, the second gear 52 and the second toothed ring 312 are sequentially meshed, so that the heat-conducting plate 23 and the object placing table 31 synchronously rotate and stop, and each time of stopping, the embryo culture dish 12 and the sample culture dish 13 which are associated with each other are close to each other for sampling, so that the relevance of samples after sampling is ensured, and the matching error of the detection result is avoided.
The top surface of the heat conducting plate 23 is provided with a plurality of first storage grooves 24, the top surface of the storage table 31 is provided with a plurality of second storage grooves 32, the first storage grooves 24 and the second storage grooves 32 are the same in number and are respectively arranged at equal intervals along the circumferential directions of the heat conducting plate 23 and the storage table 31, embryo culture dishes 12 needing PGS detection are respectively placed in the first storage grooves 24, the heating plate 22 can ensure proper temperature required by embryo culture, the sample culture dishes 13 are respectively placed in the second storage grooves 32, and the first storage grooves 24 and the second storage grooves 32 are provided with mutually symmetrical notches 6. The first storage grooves 24 and the second storage grooves 32 with the same quantity can ensure one-to-one correspondence between the embryo culture dish 12 and the sample culture dish 13 when sampling is carried out, and the gaps 6 which are symmetrical to each other are arranged, so that the culture dishes can be conveniently placed and taken out.
The top surface of the heat-conducting plate 23 is provided with a plurality of groups of signal lamp groups 7 corresponding to the first storage grooves 24 respectively, each group of signal lamp groups 7 comprises two signal lamps with different colors of light, the signal lamps respectively represent embryo normal and embryo abnormal, the top surface of the storage table 31 is provided with a plurality of groups of switch groups 8 corresponding to the second storage grooves 32 respectively, each switch group 8 comprises two switches, the switch groups 8 and the signal lamp groups 7 are connected in a one-to-one correspondence manner through electric signals, and in the correspondingly connected switch groups 8 and signal lamp groups 7, the two switches are connected in a one-to-one correspondence manner with the two signal lamps. The signal lamp set 7 is used for distinguishing and marking normal embryos and abnormal embryos so as to be convenient for doctors to identify and avoid error recording, and the specific operation is that after a sample in a sample culture dish 13 is detected, a switch representing the normal detection of the sample in the switch set 8 is pressed down according to the detection result if the sample is normal, a signal lamp representing the normal embryo in the corresponding signal lamp set 7 is lightened at the moment, the embryo culture dish 12 is marked as normal, a switch representing the abnormal detection of the sample in the switch set 8 is pressed down on the contrary, the signal lamp representing the abnormal embryo is lightened, the embryo culture dish 12 is marked as abnormal, after all the samples are detected, the embryos marked as normal in the embryo culture dish 12 are selected for transplantation, and the rest normal embryos can be continuously refrigerated and stored.
The working principle is that the device is firstly fixed, the object placing table 31 is close to a position which is convenient for a microscope to observe, for example, the starting motor 43 drives the heat conducting plate 23 and the object placing table 31 to rotate, under the control of the optocoupler baffle and the photoelectric switch 11, when one of the first object placing groove 24 and the second object placing groove 32 is close, rotation is stopped, embryo sampling in the embryo culture dish 12 is placed in the corresponding sample culture dish 13, after all embryos are sampled, each sample culture dish 13 is sequentially and directly detected by the detecting instrument, each sample is detected to perform normal or abnormal marking on the corresponding embryo condition, after detection, normal embryo transplantation is performed on the selected marking, and the rest normal embryos are refrigerated and stored.
The present embodiment is only for explanation of the present invention and is not to be construed as limiting the present invention, and modifications to the present embodiment, which may not creatively contribute to the present invention as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present invention.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202410996307.5A CN118620727B (en) | 2024-07-24 | 2024-07-24 | Preimplantation genetic diagnosis embryo biopsy device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202410996307.5A CN118620727B (en) | 2024-07-24 | 2024-07-24 | Preimplantation genetic diagnosis embryo biopsy device |
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| CN118620727A CN118620727A (en) | 2024-09-10 |
| CN118620727B true CN118620727B (en) | 2025-01-24 |
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| CN113322185A (en) * | 2021-07-16 | 2021-08-31 | 北京大学深圳医院 | Embryo biopsy device for genetic diagnosis before embryo implantation |
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| US7915034B2 (en) * | 2006-06-29 | 2011-03-29 | Genx International | Apparatus and method for culturing oocytes, embryos, stem cells and cells |
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| CN210176874U (en) * | 2019-06-18 | 2020-03-24 | 漳州卫生职业学院 | Novel embryo culture shifts ware |
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| CN209560376U (en) * | 2018-11-28 | 2019-10-29 | 深圳市帝迈生物技术有限公司 | Incubate device and the sample analyser comprising it |
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