CN118581106A - Plant insect resistance related protein PeJAZ6 and its application - Google Patents

Abstract

The invention discloses a plant insect-resistant related protein PeJAZ, the nucleic acid sequence of which is shown as SEQ ID No.1, and the protein sequence of which is shown as SEQ ID No. 2. The invention also discloses application of the PeJAZ gene-containing over-expression vector in improving insect-resistant function. The invention relates to the technical field of biology, and particularly provides a plant insect-resistant related protein PeJAZ gene, which takes a poplar endogenous insect-resistant related PeJAZ gene as an entry point, explores an insect-resistant function of PeJAZ, and can provide a new gene resource for poplar insect-resistant breeding.

Description

Plant insect resistance related protein PeJAZ6 and its application

Technical Field

The present invention relates to the field of biotechnology, and in particular to a plant insect resistance-related protein PeJAZ6 and an application thereof.

Background Art

Poplar (Populus L.) is one of the main tree species in forest regeneration and succession. It is widely distributed in the northern hemisphere due to its wide variety, fast growth, high yield, wide adaptability, and rich genetic diversity. It plays an indelible role in the ecosystem of the entire earth. Poplars can not only be used as fuel and wood producers, but also as an important part of the natural ecosystem. The poplar industry develops rapidly and grows rapidly, and it is easily eroded by pests during its growth and development. In North America, there are at least 300 species of pests and mites that harm poplars, while in Europe, there are about 250 species of pests that harm poplars. With the continuous expansion of the planting area of poplars, due to the single tree species and simple forest appearance, pests such as poplar boat moths, poplar tooth moths, and poplar longhorn beetles often occur on a large scale, causing large-scale death of poplar forests, which is becoming increasingly serious, and often breaks out on a large scale, which to a certain extent hinders the development of the poplar industry. Moreover, with the frequent occurrence of catastrophic weather in my country, it has provided a basis for the outbreak of large-scale diseases and insect pests of poplars, thus causing immeasurable losses to the growth and development of trees and the forest ecological environment. At present, the scale of poplar diseases and insect pests in my country is still expanding rapidly, and the pests are of various types and difficult to eradicate, which seriously affects the green development of poplar forest resources. Therefore, research on the insect-resistant function of poplar transgenics is imminent.

Jasmonic acid substances are divided into jasmonic acid (Jasmonate, JA) and methyl jasmonate (Methyle Jasmonate, MeJA). Among them, JA genes can regulate the expression of many genes: one is a gene related to the defense system, which is characterized by responding to drought osmotic stress and other abiotic stresses, fungal infection, pests and diseases and other biotic stresses, and the other gene is involved in plant development, such as storage protein accumulation, pollen fertility, development of different organs, seed germination, etc. In addition to affecting plant growth and development, jasmonic acid substances are also closely related to plant stress resistance. They can regulate the expression of genes related to plant stress resistance, thereby freeing plants from biotic or abiotic stresses. Jasmonic acid compounds can also induce plants to secrete defense proteins and play a regulatory role in the growth and development of insects. When JA protease is sprayed on plants, chemicals that inhibit the activity of these enzymes are produced in the plant body, which restricts the growth and development of insects and can even lead to the death of some herbivorous insects. The literature "Effects of Methyl Jasmonate on Terpene Volatiles in Masson Pine Needles and Growth and Development of Masson Pine Dendrolimus" showed that three concentrations of MeJA were used to spray Masson Pine needles and feed Masson Pine Dendrolimus punctatus Walker. The results showed that with the increase of MeJA protein concentration, the food intake, feces output, food consumption and utilization index of Masson Pine Dendrolimus punctatus Walker larvae decreased, but the larval mortality rate was the opposite; these results show that jasmonic acid compounds in plants can improve the insect resistance of plants.

JAZ (jasmonate ZIM-domian) protein is a member of the TIFY transcription factor superfamily and plays a role as a negative regulator in the jasmonic acid signal transduction pathway. It contains two conserved domains, namely the N-terminal ZIM domain (also known as TIFY) and the C-terminal Jaz domain (also known as CCT_2). Studies have shown that the OsJAZ9 gene can regulate rice's tolerance to potassium deficiency and salt stress. The study "Analysis of the Response of the Tomato JAZ Family to Pathogen Induction and Interactions between Members" showed that JAZs transcription factors only appear in plants. This subfamily is playing a vital role in regulating plant growth and development and coping with external stress, especially in the jasmonic acid signaling pathway. The study "Identification and Analysis of the JAZ Gene Family in the Sweet Potato Genome" showed that the JAZ family has a high expression level under sweet potato vine cutting disease and low temperature stress. The study "Effects of Methyl Jasmonate on Physiology of Grapes in Facility Delayed Cultivation in Alpine Regions" showed that the use of exogenous JA protein can increase the activity of antioxidant enzymes, while increasing the synthesis of antioxidants and defense compounds to induce and improve the cold resistance of watermelon, tomato and grapes. Therefore, the JAZ family can effectively improve the resistance of plants to their own and external environmental stresses, but there are few reports on the function of poplar JAZ genes in regulating insect resistance.

Summary of the invention

In view of the above situation, in order to make up for the above existing defects, the present invention provides a plant insect resistance related protein PeJAZ6, which is used to solve the problem that traditional breeding methods are difficult to cultivate new insect-resistant poplar varieties. The use of genetic engineering to cultivate transgenic insect-resistant new varieties has become a research hotspot. However, the insect-resistant genes used in the currently cultivated transgenic insect-resistant poplars are mostly derived from other species, and there are factors such as unsatisfactory insect-resistant effects or uncertain safety, which limits their application.

The present invention provides the following technical invention: a plant insect resistance-related protein PeJAZ6 proposed by the present invention, the nucleic acid sequence of the PeJAZ6 is shown in SEQ ID No. 1, and is specifically as follows:

ATGTCTGGCTCGACGGAATTCGTTGAAAGATGGGAAAGATGTGCGAGAAGCCGAGCTTCTCGCAAACTTGTAGTCTGTTGAGTCAATACTTGAAGGAGAGAGGTAGTTTCGGATCTTAATCTTGGCATGGCATCCAACAGCGAATCAACCCCGAGTAAAAACGGTCCGTCTGAGATGCTGCGTCGCTCCCCGTCTACCATGAATCTGTTTCCAGTGAGCGAGAAACCAGGTCACATTTCGTGCCAAAACAT GGGGGCTCCTCGGAATTTTACATCAATGGATTTGTTCCCTCAACAAGCTGGATTTGCTCCCAAGGAAGATGTCCCAAAGAAACTTGACTCAAGCAAGTCTGCCACCGCAGAACCCCAAACTGCGCAAATGACTATATTCTATGCCGGAAGAGTTATTGT CTTCAACGATTTCCCGGCTGACAAGGCTAAGGAAGTGATGCTCTTAGCCAGCAAGGGAAGCTCCCAGATCCAGAATGCCTTTCCTTCTATTCCAGCCAACAGTCACCCTGCCCTCGTCCCTAATATATCAAAAACTCCAATTGAGTCCACCATTTCAATTCCGTCTAGCTCAAATGCTCTTCCAAATTTTGGCAATAACTTGATTCAAGAGTGCATGCAACCATCCCTCAACCTATAGCTAATGATCTACCAATTGCAAATT GGAGAGCTTCCCTCCACCGGTTTTTGGAGAAGAGAAAAGACAGGATCATCGCAAAGGCTCCATACCAAATAAATCCTGCAGCAACTACGTCTAAGCCAGCTGAAAGCGAGTTCTCGTGGCTCGGCTTGGCTGCTCCATCTACAACACACTAG;

The protein sequence of PeJAZ6 is shown in SEQ ID No. 2, and is as follows:

MSGSTEFVEKMGKMCEKPSFSQTCSLLSQYLKERGSFGDLNLGMASNSESTPSKNGPSEMLRRSPSTMNLFPVSEKPGHISCQNMGAPRNFTSMDLFPQQAGFAPKEDVPKKLDSSKSATAEPQTAQMTIFYAGRVIVFNDFPADKAKEVMLLASKGSSQIQNAFPSIPANSHPALAPNISKTPIESTISIPSSSNALPNFGNNLIQECMQPSPQPI ANDLPIARRASLHRFLEKRKDRIIAKAPYQI NPAATTSKPAESEFSWLGLAAPSTTH.

At the same time, the present invention also proposes the application of the plant insect resistance related protein PeJAZ6 gene, specifically, the overexpression transgenic plants of the PeJAZ6 gene can improve the resistance to cotton bollworm.

The specific technical solution is described as follows:

(1) Cloning of PdJAZ6 gene

The PtJAZ6 gene (Potri.006G139400) in the genome of Populus trichocarpa was used as the reference sequence, and primers were designed using Oligo 7 software (PeJAZ6-F: ATGTCTGGCTCGACGGAATTC; PeJAZ6-R: CTAGTGTGTTGTAGATGGAGCAGCC). Total RNA was extracted from the leaves of Populus 107, reverse transcribed into cDNA, and the target gene was amplified. PCR products were detected by 1% agarose gel electrophoresis, and the purified products were recovered by gel recovery kit. The purified products were connected to the T vector, transformed into Escherichia coli DH5α competent cells, and coated on LB culture plates containing ampicillin antibiotics. Positive clones were identified by bacterial liquid PCR and sequenced.

(2) Construction of PdJAZ6 gene overexpression vector

Extract the E. coli plasmid containing the PeJAZ6 gene. Design primers for constructing the overexpression vector (upstream primer: gagaacacgggggactctagaATGTCTGGCTCGACGGAATTC; downstream primer: cgatcggggaaattcgagctcCTAGTGTGTAGATGGAGCAGCCAA), and amplify the target gene from the above plasmid using a high-fidelity enzyme. Use XbaI and SacI restriction endonucleases to double-digest the overexpression vector pROKII, and cut the gel to recover the vector fragment. Connect the above PCR fragment and the vector fragment, and transform the recombinant product into E. coli DH5α competent cells by heat shock method, and use a sterile coating stick to apply the bacterial solution on an LB culture plate containing kanamycin resistance. The positive clones screened out were identified by PCR and sequencing to ensure that the PeJAZ6 gene had been correctly inserted into the expression vector. A plasmid extraction kit was used to extract high-quality expression vector plasmids containing the target gene. The plasmids were transferred into Agrobacterium (GV3101) competent cells, resistant colonies were screened, and it was verified whether the Agrobacterium successfully carried the expression vector. The positive clones were stored at -80°C.

(3) Leaf disc tobacco genetic transformation and seedling transplanting

Take out the Agrobacterium vector stored in the -80℃ ultra-low temperature refrigerator, take out 100μL of bacterial solution, shake the bacteria overnight on a shaker at 28℃ and 150r/min until the OD value is 0.6, transfer the bacterial solution to a 50mL centrifuge tube and centrifuge. Discard the bacterial solution and transfer the Agrobacterium block to the resuspension solution for tobacco transformation. Select tobacco seedlings with good growth status and bright green leaves, take their leaves and remove the main veins, cut them into a size of about 1cm×1cm, place the cut leaves in the infection solution for infection for 10 minutes, and gently shake the liquid during the period to make the leaves fully contact with the bacterial solution; take out the infected leaves, use absorbent paper (sterilized by high pressure) to absorb the excess bacterial solution on the surface, spread them on a tobacco differentiation medium without antibiotics, and co-cultivate them under dark conditions for 36 to 48 hours; after the co-cultivation is completed, transfer the tobacco leaves to a screening differentiation medium for continued cultivation, cultivate them under the conditions of 25°C, 2000lx, and a light/dark cycle of 16h/8h, and replace the culture medium once every 2 weeks; when tender shoots grow on the tobacco leaves to about 1cm, transfer the bright green resistant shoots to a screening rooting medium for continued screening until adventitious roots grow. When the adventitious roots of tobacco seedlings grow to about 1 cm, select transgenic tobacco seedlings with good growth status, open the sealing film in a ventilated place to harden the seedlings for 3 days, take the seedlings out of the tissue culture bottle, wash the culture medium of the roots with clean water to keep the integrity of the root system as much as possible, plant them in a flowerpot with garden soil: nutrient soil: vermiculite = 1:1:1, and cultivate them in a greenhouse. In the early stage, cover the seedlings with plastic wrap to maintain high humidity to prevent the seedlings from wilting and dying due to water loss. When the seedlings have new leaves, gradually remove the film to allow the seedlings to gradually adapt to the greenhouse environment.

(4) Identification of transgenic plants

Select the leaves growing robustly in the same part of the transgenic tobacco, and extract the DNA of the transgenic tobacco by the CTAB method. Use a pair of specific primers to perform PCR detection on the transgenic strains to determine whether the target gene PeJAZ6 is inserted into the tobacco genome. In addition, extract the total RNA of the control tobacco and transgenic tobacco, then reverse transcribe it into cDNA, design fluorescence quantitative primers, configure fluorescence quantitative reaction solution, and use real-time fluorescence quantitative detector for fluorescence quantitative detection. Use the housekeeping gene Actin gene in tobacco as the internal reference to standardize the data.

DNA detection primers: pROKII-JAZ6-F: gagaacacgggggactctagaATGTCTGGCTCGACGGAATTC; pROKII-JAZ6-R: cgatcggggaaattcgagctcCTAGTGTGTTGTAGATGGAGCAGCC

Fluorescence quantitative primers: PeJAZ6-qF1: ACAGCGAATCAACCCCGAAT; PeJAZ6-qR1: TGACCTGGTTTTCTCGCTCAC

Internal reference gene primers: Actin-F: TGGACTCTGGTGATGGTGTC; Actin-R: CCTCCAATCCAAACACTGTA.

(5) Growth index determination and insect feeding experiment

For growth index determination, after 5 weeks of transplanting tobacco seedlings in the greenhouse, the plant height of each strain was measured with a ruler and the ground diameter was measured with a vernier caliper. The measured data were sorted and analyzed, and each measurement was repeated three times. For the feeding experiment, cotton bollworm (Helicoverpa armigera) was purchased from Keyun Biology (Henan) Co., Ltd. After the purchased cotton bollworm was fed in an incubator for 24 hours, tobacco leaves with strong growth were selected for feeding experiments, and photos were taken after 0 hours, 4 hours, 6 hours and 8 hours, and each treatment was repeated three times.

(6) Determination of antioxidant enzymes SOD and POD content

SOD activity assay: Weigh 0.1g tissue, add extract, centrifuge and take supernatant for subsequent testing. Add reagents according to the instructions of the SOD activity assay kit and mix well. Measure the absorbance of each tube at 450nm. SOD activity (U/g mass) = 10×inhibition percentage ÷ (1-inhibition percentage) ÷ W×D. In the formula, W represents the total sample volume, and D represents the sample dilution multiple.

POD activity assay: Weigh 0.1g tissue, add extract solution, centrifuge and take supernatant for subsequent tests; add reagents according to the instructions of the POD activity assay kit and mix well, immediately take 200μL and read the absorbance value A1 at 470nm and the absorbance value A2 after 1min. POD activity (ΔOD470/min/g) = 100×ΔA÷W. In the formula, ΔA represents the absorbance change value, and W represents the sample mass.

The content was determined using SOD and POD assay kits produced by Suzhou Grace Biotechnology Co., Ltd. The specific steps refer to the kit instructions.

The present invention proposes a plant insect resistance-related protein PeJAZ6 and its application. The beneficial effects achieved by the present invention using the above scheme are as follows:

Based on a pest-induced high-profile expressed PeJAZ6 gene screened from the transcription of gypsy moth larvae feeding on Populus occidentalis leaves, this study constructed an overexpression vector to genetically transform tobacco and obtained overexpression transgenic tobacco.

The growth and physiological indices of transgenic tobacco were measured and insect feeding experiments were conducted using transgenic tobacco to verify whether the PeJAZ6 gene has insect resistance.

This study revealed the insect resistance function of the poplar PeJAZ6 gene, providing new genes and theoretical guidance for genetically improving poplar insect resistance through transgenic technology in the future. Taking the poplar endogenous insect resistance-related PeJAZ6 gene as the starting point, exploring the insect resistance function of PeJAZ6 can provide new gene resources for poplar insect resistance breeding.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 is a diagram of gene cloning and bacterial solution PCR detection;

Fig. 2 is a PCR detection diagram of the target gene;

FIG3 is a graph showing the relative expression levels of control tobacco and different strains;

FIG4 is an analysis diagram of cotton bollworm's compulsory feeding on transgenic PeJAZ6 tobacco;

FIG5 is a diagram showing the effect of insect feeding stress on PeJAZ6 transgenic tobacco POD;

FIG. 6 is a graph showing the effect of insect feeding stress on SOD in transgenic PeJAZ6 tobacco.

The meanings of the attached drawings are as follows:

In Figure 1, Figure (A) is the electrophoresis of the PdJAZ6 gene cloned from Populus occidentalis 107; Figure (B) is the detection of the cloned vector transformed into Escherichia coli; Figure (C) is the detection of the overexpression vector transformed into Agrobacterium. The first lanes from the left of Figure 1A-C are all 2000bp DNA markers (from top to bottom 2000, 1000, 750, 500, 250, 100bp), and the remaining lanes are all PCR amplified target gene bands.

In Figure 2, M is a 2000 bp DNA marker (2000, 1000, 750, 500, 250, 100 bp from top to bottom), CK+ is a positive control (plasmid), and CK- is a negative control (wild-type tobacco WT). J1, J2, J3, J4, J5, and J6 are six transgenic tobacco lines.

In Figure 3, different lowercase letters in multiple comparisons indicate significant differences among different strains.

DETAILED DESCRIPTION

The following will clearly and completely describe the technology in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

Example

In this example, the plant insect resistance-related protein PeJAZ6 gene provided in this scheme was subjected to expression analysis, detection and related insect resistance tests.

(1) Cloning of PeJAZ6 gene and vector construction

The CDS sequence of the PeJAZ6 gene was cloned from "European and American Poplar 107", and its complete ORF region was 825bp, encoding 274 amino acids. Figure 1A is an electrophoresis diagram of the cloned product, and the size is consistent with expectations. The amplified product was constructed into a cloning vector, transformed into Escherichia coli, and the single clone was tested by bacterial liquid PCR. The electrophoresis results are shown in Figure 1B. The target band is clear and the position is accurate. The Escherichia coli with the correct band was sent to the company for sequencing. The plasmid of the bacterial liquid with correct sequencing was extracted, and the plasmid was used as a template to PCR amplify the PeJAZ6 gene fragment, which was connected to the overexpression vector pROKII after double digestion with XbaI and SacI, transformed into Escherichia coli, extracted the positive clone plasmid and transformed into Agrobacterium, and a single Agrobacterium colony was picked for target gene PCR verification. The results showed that the position of the target band was accurate, proving that the recombinant plasmid pROKII-PeJAZ6 was successfully transferred into Agrobacterium (Figure 1C).

(2) Identification and detection of transgenic tobacco overexpressing PeJAZ6

The tobacco genetic transformation was carried out by Agrobacterium-mediated leaf disc method, and a total of 6 overexpression transgenic tobacco lines were obtained. The DNA of transgenic tobacco was extracted by CTAB method, and PCR detection was performed on the 6 tobacco lines using specific primers pROKII-JAZ6-F and pROKII-JAZ6-R to identify whether the overexpression vector containing the target gene PeJAZ6 was inserted into the tobacco genome. During the experiment, the plasmid containing the target gene was used as a positive control (CK+), and the control wild-type tobacco was used as a negative control (CK-).

As shown in Figure 2, after the target gene is inserted into the genome, the target fragment can be amplified in the transgenic tobacco strain and the positive control, while the negative control cannot amplify the target fragment. After testing, the target gene has been confirmed to be transferred into 6 tobacco strains and can be used for subsequent experiments.

As shown in the qRT-RCR experiment in Figure 3, the relative expression levels of the transgenic PeJAZ6 gene in the overexpression tobacco lines J1, J2, and J6 were 38, 51, and 17 times higher than those in the control tobacco, respectively. The overexpression tobacco lines J1 and J2 were significantly higher than the control tobacco, and the overexpression tobacco line J2 had the highest relative expression level of the transgenic PeJAZ6 gene.

(3) Determination of plant height and ground diameter of transgenic tobacco

Five weeks after the transgenic tobacco was transplanted into the greenhouse, the data on plant height and ground diameter were recorded and counted. As shown in Table 1, the plant height of the control tobacco was the highest and significantly different from that of the transgenic strains. There was a significant difference between the transgenic strains J1, J2 and J6, and there was no significant difference in plant height between the transgenic strains J1 and J2. The ground diameter of the control tobacco was the thickest, which was significantly thicker than that of the transgenic tobacco strains. There was a significant difference between the ground diameter of the control tobacco and the transgenic strains, and there was no significant difference between the transgenic strains. The plant height and ground diameter of the tobacco transgenic with PeJAZ6 gene were lower than those of the control tobacco, and the growth of plant height and ground diameter of the tobacco strains was significantly inhibited.

Table 1 Plant height and ground diameter of PeJAZ6 transgenic strains

(4) Transgenic tobacco insect feeding experiment

Leaves of control tobacco and transgenic tobacco lines (J1, J2, and J6) with the same leaf area were placed in culture dishes, and the leaf area defects of the tobacco in the culture dishes were observed at different times.

As shown in Figure 4, in the same period of time, the leaf area of the control tobacco was most seriously damaged compared with the transgenic tobacco strains, and the leaf area of the transgenic tobacco strain J6 was less damaged compared with other transgenic tobacco strains, indicating that the insect resistance of the J6 strain was more significant.

(5) Effects of insect feeding stress on transgenic PeJAZ6 tobacco PODs:

POD activity is one of the key enzymes in the plant enzymatic defense system, improving the plant's ability to defend against adverse environmental factors.

As shown in Figure 5, the POD activity of transgenic tobacco and control tobacco was low before insect feeding stress, and increased significantly after the insect feeding test; there was a significant difference in POD activity between transgenic tobacco and control tobacco.

(6) Effects of insect feeding stress on SOD in transgenic PeJAZ6 tobacco:

SOD activity is an antioxidant enzyme, but it contains metal coenzymes that have the ability to eliminate superoxide anion free radicals, thereby reducing the damage of free radicals to plants.

As shown in Figure 6, the SOD activity of transgenic tobacco lines J1 and J6 increased after insect feeding stress; after insect feeding stress, the SOD activity of the control tobacco and transgenic line J2 decreased; the SOD activity of the J6 plant was the strongest.

It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, material or apparatus including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, material or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

1. Plant insect resistance related protein PeJAZ6, characterized in that the nucleic acid sequence of PeJAZ6 is shown in SEQ ID No.1, specifically as follows: ATGTCTGGCTCGACGGAATTCGTTGAAAGATGGGAAAGATGTGCGAGAAGCCGAGCTTCTCGCAAACTTGTAGTCTGTTGAGTCAATACTTGAAGGAGAGAGGTAGTTTCGGATCTTAATCTTGGCATGGCATCCAACAGCGAATCAACCCCGAGTAAAAACGGTCCGTCTGAGATGCTGCGTCGCTCCCCGTCTACCATGAATCTGTTTCCAGTGAGCGAGAAACCAGGTCACATTTCGTGCCAAAACAT GGGGGCTCCTCGGAATTTTACATCAATGGATTTGTTCCCTCAACAAGCTGGATTTGCTCCCAAGGAAGATGTCCCAAAGAAACTTGACTCAAGCAAGTCTGCCACCGCAGAACCCCAAACTGCGCAAATGACTATATTCTATGCCGGAAGAGTTATTGT CTTCAACGATTTCCCGGCTGACAAGGCTAAGGAAGTGATGCTCTTAGCCAGCAAGGGAAGCTCCCAGATCCAGAATGCCTTTCCTTCTATTCCAGCCAACAGTCACCCTGCCCTCGTCCCTAATATATCAAAAACTCCAATTGAGTCCACCATTTCAATTCCGTCTAGCTCAAATGCTCTTCCAAATTTTGGCAATAACTTGATTCAAGAGTGCATGCAACCATCCCTCAACCTATAGCTAATGATCTACCAATTGCAAATT GGAGAGCTTCCCTCCACCGGTTTTTGGAGAAGAGAAAAGACAGGATCATCGCAAAGGCTCCATACCAAATAAATCCTGCAGCAACTACGTCTAAGCCAGCTGAAAGCGAGTTCTCGTGGCTCGGCTTGGCTGCTCCATCTACAACACACTAG; The protein sequence of PeJAZ6 is shown in SEQ ID No. 2, and is as follows: MSGSTEFVEKMGKMCEKPSFSQTCSLLSQYLKERGSFGDLNLGMASNSESTPSKNGPSEMLRRSPSTMNLFPVSEKPGHISCQNMGAPRNFTSMDLFPQQAGFAPKEDVPKKLDSSKSATAEPQTAQMTIFYAGRVIVFNDFPADKAKEVMLLASKGSSQIQNAFPSIPANSHPALAPNISKTPIESTISIPSSSNALPNFGNNLIQECMQPSPQPI ANDLPIARRASLHRFLEKRKDRIIAKAPYQINPAATTSKPAESEFSWLGLAAPSTTH. 2. The plant insect resistance-related protein PeJAZ6 according to claim 1, characterized in that the PCR specific primer pair used to clone PeJAZ6 is: The nucleic acid sequence of the upstream primer is shown in SEQ ID No. 3: ATGTCTGGCTCGACGGAATTC; The nucleic acid sequence of the downstream primer is shown in SEQ ID No. 4: CTAGTGTGTTGTAGATGGAGCAGCC. 3. The plant insect resistance-related protein PeJAZ6 according to claim 1, characterized in that the primers used for amplification and construction of the PeJAZ6 overexpression vector are: The nucleic acid sequence of the upstream primer is shown in SEQ ID No.5: gagaacacgggggactctagaATGTCTGGCTCGACGGAATTC; The nucleic acid sequence of the downstream primer is shown in SEQ ID No.6: cgatcggggaaattcgagctcCTAGTGTGTAGATGGAGCAGCCAA. 4. An overexpression vector containing the plant insect resistance-related protein PeJAZ6 according to any one of claims 1 to 3. 5. The use of the plant insect resistance-related protein PeJAZ6 according to claim 4, characterized in that an overexpression vector containing the PeJAZ6 gene is used to improve the insect resistance function. 6. The use of the plant insect resistance-related protein PeJAZ6 according to claim 5, characterized in that the transgenic plants overexpressing the PeJAZ6 gene can improve resistance to cotton bollworm.