CN118501432B - Brucellosis CF-ELISA Antibody Detection Kit - Google Patents

Abstract

The invention discloses a brucellosis CF-ELISA antibody detection kit, which relates to the technical field of biological detection, wherein a rotating shaft is arranged at the center in a circular box body, a radially telescopic mounting plate capable of turning over by 180 degrees is arranged at the top of the rotating shaft, a supporting plate capable of shaking up and down is elastically arranged on the side wall of the mounting plate, an ELISA plate is magnetically attracted on the supporting plate, the front side of the circular box body is provided with an up-down material level, the left side is provided with a liquid moving level, the rear side is provided with a washing position, the right side is provided with an incubation position, the circular box body is provided with a material opening, a hanging plate, a washing groove and an incubation chamber corresponding to the up-down material level, the liquid moving level, the washing position and the incubation position respectively, a plurality of rows of fixing columns used for liquid moving are fixedly arranged on the hanging plate, a plurality of sliding columns capable of longitudinally moving are also arranged on the hanging plate, and lifting pipettes are respectively arranged at the bottoms of the fixing columns and the sliding columns. The kit can integrate pipetting, washing and incubation equipment, automatically complete pipetting, washing and incubation processes, does not need to frequently replace pipetting heads, and does not need to manually and frequently operate.

Description

Brucellosis CF-ELISA antibody detection kit

Technical Field

The invention relates to the technical field of biological detection, in particular to a brucellosis CF-ELISA antibody detection kit.

Background

Brucellosis is an infectious disease of people and livestock caused by brucellosis, the first symptoms are usually acute fever, little or no local symptoms such as headache, arthralgia and the like, the early detection cure rate is higher, once the early detection is not timely progressed to a chronic stage, the abortion, the infertility and the disability of people for life are caused, and the harm is extremely great. Early diagnosis of the disease is a key of prevention and control, and a diagnosis method with high sensitivity and high specificity is particularly important.

CF-ELISA is a complement binding test which shows the antigen-antibody specific reaction result by enzyme development technique in ELISA plate holes, and has the advantages of CFT high specificity and ELISA high sensitivity, and compared with the traditional complement binding test, the CF-ELISA has the characteristics of high sensitivity, convenient operation and high flux detection, and is currently used for detecting brucellosis.

However, the steps of adopting the CF-ELISA mode are complicated, different pipetting guns are needed to transfer sample diluent, serum samples, competing antibodies, enzyme-labeled antibodies, substrate solution and stop solution onto the ELISA plate, the pipetting head needs to be replaced every time different liquids are transferred, the steps of washing and incubation are complicated, a great deal of time and effort are needed for manual operation, and false positive is easy to occur due to errors.

Disclosure of Invention

In order to solve the problems, the invention provides the brucellosis CF-ELISA antibody detection kit which can integrate pipetting, washing and incubation equipment, automatically complete pipetting, washing and incubation processes, does not need to frequently replace pipetting heads and does not need to be operated manually frequently.

In order to solve the problems, the invention adopts the following technical scheme:

The utility model provides a brucellosis CF-ELISA antibody detection kit, includes the circular box body, the central department installs the rotation axis in the circular box body, but radial flexible and 180 mounting panel that overturns are installed at the top of rotation axis, but the backup pad of upper and lower shake is installed to the lateral wall elastic mounting of mounting panel, the ELISA plate is inhaled to magnetism on the backup pad, the front side of circular box body is upper and lower material level, left side is the liquid level that moves, the rear side is the washing position, the right side is the incubation position, and the circular box body corresponds upper and lower material level, moves liquid level, washing position and incubation position punishment do not are equipped with feed inlet, hanger plate, washing tank and incubation room, but fixedly mounted with multirow is used for moving the fixed column of liquid on the hanger plate, but still install a plurality of sliding columns of longitudinal movement, but the pump liquid mechanism of pump to the pipette is installed to in fixed column and the sliding column, the top of circular box body corresponds the position fixed mounting has a plurality of liquid tanks, a plurality of liquid level tanks, liquid level that the liquid tank that moves into, the sample liquid tank that the sample is connected with the washing pipe, the liquid tank that moves up through the liquid is connected to the liquid tank, the liquid tank that moves up to the liquid is installed to the liquid tank, the liquid tank that the sample, the liquid that is connected to the liquid tank.

Preferably, the top fixed mounting of rotation axis has the rotating electrical machines, the one end fixed mounting of rotating electrical machines has electric putter, electric putter's flexible end elastic mounting has the connector, the terminal and the mounting panel fixed connection of connector.

Preferably, the telescopic end of the electric push rod is provided with a groove, the connector extends into the groove and is elastically connected with a first spring, the side wall of the mounting plate is provided with a strip-shaped groove, and one end of the supporting plate extends into the strip-shaped groove and is elastically connected with a second spring.

Preferably, the supporting plate is provided with a slot in the radial direction, the bottom of the ELISA plate is fixed with a bottom cutting adapting to the slot, an iron core is embedded in the bottom cutting, and a first electromagnetic suction plate for magnetically sucking the bottom cutting is arranged at the inner bottom of the slot.

Preferably, a plurality of longitudinal sliding ways are arranged on the hanging plate, the sliding columns are slidably arranged in the corresponding sliding ways, and electric sliding rails capable of driving the sliding columns to slide are arranged on the longitudinal inner walls of the sliding ways.

Preferably, the bottoms of the fixed column and the sliding column are respectively provided with a round cavity, the upper end of the pipette extends into the round cavity and is elastically connected with a third spring, and the round box body is provided with a controller electrically connected with a plurality of third springs.

Preferably, the liquid pumping mechanism comprises a liquid pumping cavity arranged in the top of the fixed column and the sliding column, the upper end of the pipette extends into the liquid pumping cavity and is fixedly provided with a piston, the upper end of the pipette is provided with a liquid inlet, the lower end of the pipette is connected with the corresponding liquid pumping cavity, and the pipette are internally provided with one-way valves.

Preferably, the shaking mechanism is arranged at the inner top of the circular box body corresponding to the washing position and is used for driving the ELISA plate to shake up and down or left and right.

Preferably, the shake mechanism includes the electric telescopic handle of fixing at circular box body top, electric telescopic handle's expansion end fixedly connected with is kept out the board, the lateral wall fixed mounting who keeps out the board has the motor, the output of motor is fixed with the cam, the cam cooperates with fagging and mounting panel.

Preferably, an incubation cavity is arranged in the incubation chamber, a socket for inserting an ELISA plate is arranged on the left side of the incubation cavity, a temperature control tube is arranged at the top of the incubation cavity, second electromagnetic suction plates are embedded in the inner walls of the two sides of the incubation cavity, magnetic suction plates matched with the second electromagnetic suction plates are embedded in the two sides of the ELISA plate, and an iron core is embedded in the magnetic suction plates.

The beneficial effects of the invention are as follows:

(1) Through setting up material level, shifting liquid level, washing position, incubation position and rotation axis about, only need place the ELISA plate from last material level down on the fagging, through rotation axis according to detecting order automatic transfer to material level, shifting liquid level, washing position, incubation position about, automatic completion shifting, washing and incubation process, take out the ELISA plate at last and can send into in the ELISA instrument and detect, realize automatic testing process.

(2) Through installing multirow fixed column, a plurality of sliding columns, the pipette, pump liquid mechanism, a plurality of dress cistern and electric putter, sample diluent that will detect needs, the serum sample, competing antibody, the enzyme-labeled antibody, substrate solution and termination solution pack into a plurality of dress cistern respectively, after the enzyme-labeled plate changes to the liquid level, multirow enzyme-labeled hole on the enzyme-labeled plate passes through the pipette of one row by row through electric putter, third spring circular telegram through controller control one row, make its shrink drive pipette move down insert in the enzyme-labeled hole of corresponding one row, drive the piston move down when descending, can be with the sample diluent that corresponds in the pump liquid chamber pour into multirow enzyme-labeled hole respectively, after the liquid is accomplished to one row, the pipette drives the piston and resets, can inhale the sample diluent in the pump liquid intracavity that corresponds through the pipette again, remaining multirow pipette can accomplish the liquid process of pouring of corresponding liquid respectively, a plurality of sliding columns can be under the drive of electric slide rail longitudinal movement, and then can pour into the negative control, serum positive control serum and serum sample into the corresponding enzyme-labeled hole of corresponding hole alone respectively, can replace the cross-over pipette to take place frequently and replace the pipette to take place frequently.

(3) Through installation wash tank, rotating electrical machines and a row of wash pipe, accomplish the injection of competing antibody and enzyme-linked immunosorbent assay on the ELISA plate and accomplish the incubation process after, all need carry out a lot of wash process, only need rotate the ELISA plate to the washing position this moment, start the rotating electrical machines, drive the fagging through electric putter and mounting panel and rotate 180, make multirow enzyme-linked pore down, pump the washing liquid through the feed liquor pipe pump, the rethread wash pipe upwards spouts, the drive next row of enzyme-linked pore row by row through a row of wash pipe at electric putter, can wash downthehole liquid, accomplish the wash process, need not artificial washing.

(4) Through installation incubation room, first electromagnetism is inhaled board, bottom cutting, a pair of magnetism is inhaled board and second magnetism and is inhaled the board, shake in-process about radial shake and, the equal magnetism of first magnetism is inhaled the board and is inhaled the bottom cutting, realize the magnetism of ELISA plate and inhale fixedly, when carrying out the incubation, the ELISA plate changes to the incubation position, start electric putter and insert the ELISA plate Wen Yoqiang through the socket in, inhale the board through the magnetism of second electromagnetism and inhale the ELISA plate both sides, first electromagnetism is inhaled board release bottom cutting, can send into the incubation intracavity with the ELISA plate voluntarily, realize constant temperature cultivation process through the control by temperature change pipe.

(5) After the competitive antibody and the enzyme-labeled antibody are injected on the enzyme-labeled plate, before incubation, the enzyme-labeled plate is turned to a washing position, an electric telescopic rod is started to drive a bearing plate to descend, and then an electric push rod is started to drive the enzyme-labeled plate to move radially, so that a cam abuts against a mounting plate, the mounting plate always abuts against the cam under the action of a first spring, a motor is started to drive the cam to rotate, the mounting plate can be driven to shake radially and reciprocally, and further the enzyme-labeled plate is driven to shake radially and reciprocally through a supporting plate, so that a shaking process is realized without shaking by hands; after washing, the electric telescopic rod can be started to drive the supporting plate to descend, so that the cam is abutted against the supporting plate, and under the action of the second spring, the cam can rotate to drive the ELISA plate to shake up and down, and liquid in the hole can be further thrown out.

Drawings

FIG. 1 is a perspective view of a brucellosis CF-ELISA antibody detection kit of the invention;

FIG. 2 is a cross-sectional view of FIG. 1;

FIG. 3 is a schematic view of the structure of the connector, mounting plate and brace plate according to the present invention;

FIG. 4 is a left-right cross-sectional view of FIG. 1;

FIG. 5 is a cross-sectional view of a hanger plate in accordance with the present invention;

FIG. 6 is a top view of a hanger plate according to the present invention;

FIG. 7 is a front-rear cross-sectional view of the present invention;

FIG. 8 is a schematic diagram of a dithering mechanism according to the present invention;

fig. 9 is a schematic diagram of an up-down jitter state according to the present invention;

fig. 10 is a schematic diagram of a shaking-up state according to the present invention.

In the figure: 1. a circular box body; 2. a material port; 3. shifting the liquid level; 4. a washing station; 5. incubating the site; 6. discharging the material; 7. a rotation shaft; 8. a rotating electric machine; 9. an electric push rod; 10. a mounting plate; 11. an ELISA plate; 12. a magnetic suction plate; 13. a hanger plate; 14. a washing tank; 15. a washing tube; 16. a liquid inlet pipe; 17. an incubation chamber; 18. wen Yoqiang; 19. a first spring; 20. a connector; 21. a second spring; 22. a supporting plate; 23. bottom cutting; 24. a first electromagnetic suction plate; 25. a slot; 26. fixing the column; 27. a liquid loading groove; 28. a pipette; 29. a socket; 30. a second electromagnetic suction plate; 31. a temperature control tube; 32. a sliding column; 33. an electric slide rail; 34. a slideway; 35. a pipette; 36. a circular cavity; 37. a third spring; 38. a pump liquid cavity; 39. a piston; 40. an electric telescopic rod; 41. a retaining plate; 42. a cam; 43. and a motor.

Detailed Description

Example 1

In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit or scope of the invention, which is therefore not limited to the specific embodiments disclosed below.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.

Referring to fig. 1-10, the brucellosis CF-ELISA antibody detection kit comprises a circular box body 1, a rotary shaft 7 is installed at the center in the circular box body 1, a radially telescopic mounting plate 10 capable of being turned over by 180 degrees is installed at the top of the rotary shaft 7, a supporting plate 22 capable of shaking up and down is elastically installed on the side wall of the mounting plate 10, a rotary motor 8 is fixedly installed at the top of the rotary shaft 7, the front side of the circular box body 1 is an up-down material level 6, the left side is a liquid moving level 3, the rear side is a washing level 4, the right side is an incubation level 5, the circular box body 1 is provided with a material opening 2, a hanging plate 13, a washing tank 14 and an incubation chamber 17 corresponding to the up-down material level 6, the liquid moving level 3, the washing level 4 and the incubation level 5 respectively, the enzyme label 11 is placed on the supporting plate 22 from the up-down material level 6, the liquid moving level 3, the washing level 4 and the incubation level 5 according to detection sequence, the automatic completion of the liquid moving, washing and incubation processes are carried out, and finally the enzyme label 11 is taken out, and the enzyme label 11 can be automatically detected in an enzyme label instrument to carry out detection process.

The ELISA plate 11 is magnetically attracted to the support plate 22, the slot 25 is radially formed in the support plate 22, the bottom of the ELISA plate 11 is fixedly provided with a bottom cutting 23 which is matched with the slot 25, an iron core is embedded in the bottom cutting 23, a first electromagnetic suction plate 24 for magnetically attracting the bottom cutting 23 is arranged at the inner bottom of the slot 25, the ELISA plate 11 is arranged on the support plate 22 from the upper material level 6 and the lower material level 6, the bottom cutting 23 is inserted into the slot 25, and the first electromagnetic suction plate 24 magnetically attracts the bottom cutting 23 to be fixed.

The lifting plate 13 is fixedly provided with a plurality of rows of fixed columns 26 for pipetting, a plurality of sliding columns 32 capable of longitudinally moving are also arranged on the lifting plate 13, a plurality of longitudinal slide ways 34 are arranged on the lifting plate 13, the sliding columns 32 are slidably arranged in the corresponding slide ways 34, electric slide rails 33 capable of driving the sliding columns 32 to slide are arranged on the longitudinal inner walls of the slide ways 34, the lifting pipettes 28 are arranged at the bottoms of the fixed columns 26 and the sliding columns 32, a liquid pumping mechanism capable of pumping liquid to the pipettes 28 is arranged in the fixed columns 26 and the sliding columns 32, a plurality of liquid containing tanks 27 are fixedly arranged at the top of the round box body 1 corresponding to the liquid level 3, sample diluent, serum sample, competitive antibody, enzyme-labeled antibody, substrate solution and stop solution are respectively filled in the plurality of liquid containing tanks 27, and the liquid pumping mechanism is connected with the corresponding liquid containing tanks 27 through liquid suction pipes 35, so that cross contamination can be replaced by single-hole pipetting guns and hole pipetting guns without frequently replacing pipetting heads.

Further, the bottoms of the fixed column 26 and the sliding column 32 are respectively provided with a circular cavity 36, the upper end of the pipette 28 extends into the circular cavity 36 and is elastically connected with a third spring 37, a controller electrically connected with the third springs 37 is arranged on the circular box body 1, the controller controls the third springs 37 of one row to be electrified, so that the controller contracts to drive the pipette 28 to move downwards to insert into the enzyme-labeled hole of the corresponding row for liquid injection, and a single-hole liquid injection or hole discharge liquid injection process can be performed.

Specifically, the pump liquid mechanism includes the pump liquid chamber 38 that sets up in fixed column 26 and slide column 32 top, the upper end of pipette 28 extends to pump liquid chamber 38 and is fixed with piston 39, the feed liquor hole has been seted up to the upper end of pipette 28, the lower extreme of pipette 35 is connected with corresponding pump liquid chamber 38, the check valve is all installed in pipette 35 and the pipette 28, drive piston 39 and move down when pipette 28 descends, can pour into the sample diluent that corresponds in pump liquid chamber 38 respectively in the multirow enzyme-labeled hole, after the liquid is annotated to one row, pipette 28 drives piston 39 and resets, can be through pipette 35 again with the sample diluent that corresponds in the dress cistern 27 in pump liquid chamber 38, the liquid process of annotating of corresponding liquid can be accomplished respectively to remaining multirow pipette 28, a plurality of slide columns 32 can be under the drive of electronic slide rail 33 longitudinal movement, and then can pour into negative control serum, positive control serum and the serum sample that awaits measuring respectively in the corresponding enzyme-labeled hole alone.

A row of washing pipes 15 for pumping washing liquid upwards is arranged at the notch of the washing tank 14, a liquid inlet pipe 16 connected with the row of washing pipes 15 is arranged at the rear side of the circular box body 1, a shaking mechanism is arranged at the inner top of the circular box body 1 corresponding to the washing position 4 and used for driving the ELISA plate 11 to shake up and down or left and right, the shaking mechanism comprises an electric telescopic rod 40 fixed at the inner top of the circular box body 1, the telescopic end of the electric telescopic rod 40 is fixedly connected with a retaining plate 41, a motor 43 is fixedly arranged on the side wall of the retaining plate 41, a cam 42 is fixed at the output end of the motor 43, the cam 42 is matched with a supporting plate 22 and a mounting plate 10, an electric push rod 9 is fixedly arranged at one end of the rotary motor 8, a connector 20 is elastically arranged at the telescopic end of the electric push rod 9, the tail end of the connector 20 is fixedly connected with the mounting plate 10, the telescopic end of the electric push rod 9 is provided with a groove, the connector 20 extends into the groove and is elastically connected with a first spring 19, the side wall of the mounting plate 10 is provided with a strip-shaped groove, one end of the supporting plate 22 extends into the strip-shaped groove and is elastically connected with a second spring 21, after the injection of competing antibody and the enzyme-labeled antibody is completed on the enzyme-labeled plate 11, before incubation, the enzyme-labeled plate 11 is rotated to the washing position 4, the electric telescopic rod 40 is started to drive the resisting plate 41 to descend, then the electric push rod 9 is started to drive the enzyme-labeled plate 11 to move radially, so that the cam 42 is abutted against the mounting plate 10, under the action of the first spring 19, the mounting plate 10 is always abutted against the cam 42, the starting motor 43 drives the cam 42 to rotate, so that the radial reciprocating shaking of the mounting plate 10 can be driven, and the radial reciprocating shaking of the enzyme-labeled plate 11 is driven by the supporting plate 22, so that the shaking process is realized without manual shaking; after washing, the electric telescopic rod 40 can be started to drive the abutting plate 41 to descend, so that the cam 42 abuts against the supporting plate 22, and under the action of the second spring 21, the cam 42 can rotate to drive the ELISA plate 11 to shake up and down, and liquid in the hole can be further thrown out.

An incubation cavity 18 is arranged in the incubation chamber 17, a socket 29 for inserting the ELISA plate 11 is arranged on the left side of the incubation cavity 18, a temperature control tube 31 is arranged at the top of the incubation cavity 18, second electromagnetic suction plates 30 are embedded in the inner walls of the two sides of the incubation cavity 18, magnetic suction plates 12 matched with the second electromagnetic suction plates 30 are embedded in the two sides of the ELISA plate 11, iron cores are embedded in the magnetic suction plates 12, when incubation is carried out, the ELISA plate 11 is transferred to an incubation position 5, an electric push rod 9 is started to insert the ELISA plate 11 into the incubation cavity 18 through the socket 29, the magnetic suction plates 12 on the two sides of the ELISA plate 11 are magnetically sucked through the second electromagnetic suction plates 30, the first electromagnetic suction plates 24 release bottom cuttings 23, and the ELISA plate 11 can be automatically fed into the incubation cavity 18, and a constant temperature incubation process is realized through the temperature control tube 31.

The ELISA plate 11 is arranged on the supporting plate 22 from the upper material level 6 to the lower material level 6, the bottom cutting 23 is inserted into the slot 25, the first electromagnetic suction plate 24 magnetically sucks the bottom cutting 23 for fixing, the rotation shaft 7 automatically rotates to the upper material level 6 to the lower material level 3, the washing position 4 and the incubation position 5 according to the detection sequence, the pipetting, washing and incubation processes are automatically completed, and finally the ELISA plate 11 is taken out to be sent into the ELISA instrument for detection, so that the automatic detection process is realized.

Sample diluent, serum sample, competing antibody, enzyme-labeled antibody, substrate solution and stopping solution which are needed by detection are respectively filled into the plurality of liquid filling grooves 27, after the enzyme-labeled plate 11 is transferred to the liquid filling level 3, a plurality of rows of enzyme-labeled holes on the enzyme-labeled plate 11 pass through a row of pipettes 28 one by one through the electric push rod 9, the third springs 37 in a row are controlled by the controller to be electrified, the third springs shrink to drive the pipettes 28 to move downwards and insert the corresponding rows of enzyme-labeled holes, the piston 39 is driven to move downwards while descending, the corresponding sample diluent in the liquid filling grooves 38 can be respectively filled into the plurality of rows of enzyme-labeled holes, after the liquid filling is completed in a row, the pipettes 28 drive the piston 39 to reset, the sample diluent in the corresponding liquid filling grooves 27 can be sucked into the liquid filling grooves 38 through the pipettes 35 again, the rest of the plurality of rows of pipettes 28 can be respectively completed, and the slide columns 32 can be driven by the electric slide rails 33 to move longitudinally, so that negative control serum, positive control serum and a sample to be respectively filled into the corresponding enzyme-labeled holes independently, and the single-labeled holes can replace the single-row pipette and the cross pipette can not be frequently replaced, and the pipette head is not required to be frequently replaced.

After the competitive antibody and the enzyme-labeled antibody are injected into the ELISA plate 11 and the incubation process is completed, a plurality of washing processes are required, at the moment, the ELISA plate 11 is only required to be rotated to the washing position 4, the rotating motor 8 is started, the supporting plate 22 is driven to rotate 180 degrees through the electric push rod 9 and the mounting plate 10, a plurality of rows of enzyme-labeled holes are downward, washing liquid is pumped through the liquid inlet pipe 16, the washing liquid is sprayed upwards through the row of washing pipes 15, and the liquid in the holes can be washed away by the row of washing pipes 15 under the driving of the electric push rod 9, so that the washing process is completed.

The ELISA plate 11 is turned to the incubation position 5, the electric push rod 9 is started to insert the ELISA plate 11 into the incubation cavity 18 through the socket 29, the magnetic plates 12 on two sides of the ELISA plate 11 are magnetically attracted through the second electromagnetic plate 30, the bottom cutting 23 is released by the first electromagnetic plate 24, the ELISA plate 11 can be automatically sent into the incubation cavity 18, the constant temperature incubation process is realized through the temperature control tube 31, and meanwhile, the ELISA plate is easy to take out.

After the competitive antibody and the enzyme-labeled antibody are injected on the enzyme-labeled plate 11, before incubation, the enzyme-labeled plate 11 is turned to the washing position 4, the electric telescopic rod 40 is started to drive the bearing plate 41 to descend, the electric push rod 9 is started to drive the enzyme-labeled plate 11 to move radially, the cam 42 is enabled to abut against the mounting plate 10, the mounting plate 10 always abuts against the cam 42 under the action of the first spring 19, the starting motor 43 drives the cam 42 to rotate, the mounting plate 10 can be driven to shake radially and reciprocally, and then the enzyme-labeled plate 11 is driven to shake radially and reciprocally through the supporting plate 22, so that a shaking process is realized, and even shaking is not needed by hands; after washing, the electric telescopic rod 40 can be started to drive the abutting plate 41 to descend, so that the cam 42 abuts against the supporting plate 22, and under the action of the second spring 21, the cam 42 can rotate to drive the ELISA plate 11 to shake up and down, and liquid in the hole can be further thrown out.

The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims (8)

1. The utility model provides a brucellosis CF-ELISA antibody detection kit, includes circular box body (1), its characterized in that, central department installs rotation axis (7) in circular box body (1), the top fixed mounting of rotation axis (7) has rotating electrical machines (8), the one end fixed mounting of rotating electrical machines (8) has electric putter (9), the flexible end elastic mounting of electric putter (9) has connector (20), the end and the mounting panel (10) fixed connection of connector (20), mounting panel (10) can radially stretch out and draw back and can 180 upset, but bracing plate (22) that the lateral wall elastic mounting of mounting panel (10) can shake from top to bottom, magnetically inhale on bracing plate (22) have ELISA plate (11), the front side of circular box body (1) is upper and lower material level (6), and the left side is for moving (3), and the rear side is washing position (4), and the right side is incubation position (5), and circular box body (1) corresponds upper and lower material level (6), moves (3), washing position (4) and incubation position (5) and is equipped with movable column (13) and still has a plurality of slide bearings (13) to move on the fixed column (13) in order to move the fixed column (13), the automatic cleaning device is characterized in that a plurality of slide ways (34) are arranged on the hanging plate (13), the sliding columns (32) are slidably arranged in the corresponding slide ways (34), electric slide rails (33) capable of driving the sliding columns (32) to slide are arranged on the inner walls of the slide ways (34), lifting pipettes (28) are arranged at the bottoms of the fixing columns (26) and the sliding columns (32), liquid pumping mechanisms capable of pumping liquid to the pipettes (28) are arranged in the fixing columns (26) and the sliding columns (32), a plurality of liquid loading grooves (27) are fixedly arranged at positions, corresponding to the liquid moving level (3), of the top of the round box body (1), sample diluent, serum samples, competing antibodies, enzyme-labeled antibodies, substrate solutions and terminating solutions are respectively arranged in the liquid loading grooves (27), the liquid pumping mechanisms are connected with the corresponding liquid loading grooves (27) through liquid sucking pipes (35), a row of washing pipes (15) capable of pumping washing liquid upwards are arranged at the notch positions of the washing grooves (14), and liquid inlets (16) are connected with one row of washing pipes (15) are arranged at the rear sides of the round box body (1).

2. Brucellosis CF-ELISA antibody detection kit according to claim 1, characterized in that the telescopic end of the electric push rod (9) is provided with a groove, the connector (20) extends into the groove and is elastically connected with a first spring (19), the side wall of the mounting plate (10) is provided with a strip-shaped groove, and one end of the supporting plate (22) extends into the strip-shaped groove and is elastically connected with a second spring (21).

3. Brucellosis CF-ELISA antibody detection kit according to claim 2, characterized in that the supporting plate (22) is provided with a slot (25) in the radial direction, the bottom of the ELISA plate (11) is fixedly provided with a bottom cutting (23) which is matched with the slot (25), the bottom cutting (23) is embedded with an iron core, and the inner bottom of the slot (25) is provided with a first electromagnetic suction plate (24) which is used for magnetically sucking the bottom cutting (23).

4. A brucellosis CF-ELISA antibody detection kit according to claim 3, characterized in that the bottoms of the fixed column (26) and the sliding column (32) are respectively provided with a round cavity (36), the upper end of the pipette (28) extends into the round cavity (36) and is elastically connected with a third spring (37), and the round box body (1) is provided with a controller electrically connected with a plurality of third springs (37).

5. The brucellosis CF-ELISA antibody detection kit according to claim 4, characterized in that the liquid pumping mechanism comprises a liquid pumping cavity (38) arranged in the tops of the fixed column (26) and the sliding column (32), the upper end of the pipette (28) extends into the liquid pumping cavity (38) and is fixedly provided with a piston (39), the upper end of the pipette (28) is provided with a liquid inlet hole, the lower end of the pipette (35) is connected with the corresponding liquid pumping cavity (38), and the pipette (35) and the pipette (28) are internally provided with one-way valves.

6. The brucellosis CF-ELISA antibody detection kit according to claim 5, wherein the circular box body (1) is provided with a shaking mechanism corresponding to the inner top of the washing position (4), and the shaking mechanism is used for driving the ELISA plate (11) to shake up and down or left and right.

7. The brucellosis CF-ELISA antibody detection kit according to claim 6, characterized in that the shaking mechanism comprises an electric telescopic rod (40) fixed at the inner top of the circular box body (1), a retaining plate (41) is fixedly connected with the telescopic end of the electric telescopic rod (40), a motor (43) is fixedly arranged on the side wall of the retaining plate (41), a cam (42) is fixed at the output end of the motor (43), and the cam (42) is matched with the supporting plate (22) and the mounting plate (10).

8. The brucellosis CF-ELISA antibody detection kit according to claim 7, wherein an incubation cavity (18) is arranged in the incubation chamber (17), a socket (29) for inserting an ELISA plate (11) is arranged on the left side of the Wen Yoqiang (18), a temperature control tube (31) is arranged at the top of the incubation cavity (18), second electromagnetic suction plates (30) are embedded in the inner walls of the two sides of the incubation cavity (18), magnetic suction plates (12) matched with the second electromagnetic suction plates (30) are embedded in the two sides of the ELISA plate (11), and an iron core is embedded in the magnetic suction plates (12).