CN118207066B - Culture unit, culture device and culture method for spirulina - Google Patents

Abstract

The invention discloses a culture unit, a culture device and a culture method of spirulina with salted soil, wherein the culture unit comprises a reactor main body, a reactor top cover and a culture frame, wherein the reactor main body is an incubator with an open top; the reactor top cover is detachably covered on the top of the reactor main body, is made of transparent materials and is provided with a plurality of through holes; the light source is arranged on the culture rack and is positioned above the top cover of the reactor. The culture method comprises the following steps: adding monosodium glutamate wastewater with the volume fraction of 1-2%o into seawater to prepare a culture medium solution; the culture medium solution is added into the reactor main body to make the depth of the culture medium solution be 4-5cm, and the spirulina with salt is added into the culture medium solution for culturing, so as to solve the problem of difficult scale expansion of the spirulina with salt in the prior art.

Description

A culturing unit, culturing device and culturing method for Spirulina salina

Technical Field

The invention belongs to the technical field of spirulina cultivation, and in particular relates to a salt-water spirulina cultivation unit, a cultivation device and a cultivation method.

Background Art

The statements herein merely provide background information related to the present invention and do not necessarily constitute prior art.

Spirulina is a filamentous prokaryotic organism composed of single cells or multiple cells. It is one of the microalgae that has been successfully mass-produced. The main species of Spirulina currently used commercially are Spirulina platensis and Spirulina maxima. However, Spirulina salina has a fast growth rate, high photosynthetic efficiency, and high protein content, and has the potential for large-scale production. It is crucial to find a suitable cultivation model for Spirulina salina and expand the cultivation scale.

Large-scale production of spirulina mostly uses open culture ponds, which occupy a large area, are difficult to operate manually, and are prone to biological contamination. The culture devices mostly use aeration, stirring and other methods for disturbance, which have problems such as high energy consumption, complex equipment, and difficult maintenance, which is not conducive to large-scale production and cost control.

Spirulina grows well under low light intensity, but the light will decay rapidly with the increase of water depth. It is difficult for the Spirulina at the bottom to obtain sufficient light to ensure its photosynthesis. The commonly used dispersion methods to promote the uniform distribution of microalgae in the culture container, such as aeration and stirring, will cause mechanical damage to Spirulina and are not suitable for large-scale production of Spirulina.

Summary of the invention

In view of the shortcomings of the prior art, the purpose of the present invention is to provide a culture unit, a culture device and a culture method for Spirulina salina to solve the problem of difficulty in scaling up Spirulina salina in the prior art.

In order to achieve the above object, the present invention is implemented through the following technical solutions:

In a first aspect, the present invention provides a culture unit for Spirulina salizawa, comprising a reactor body, a reactor top cover and a culture rack, wherein:

The reactor body is an incubator with an open top;

The reactor top cover is detachably covered on the top of the reactor body, the reactor top cover is made of a transparent material, and a plurality of through holes are arranged on the reactor top cover;

The light source is installed on the culture rack and is located above the top cover of the reactor.

In some embodiments, two groups of through holes are disposed on the reactor top cover, and the two groups of through holes are disposed oppositely on two sides of the reactor top cover.

Preferably, the number of through holes in each group is 2-5, and the diameter of each through hole is 2-4 cm.

In some embodiments, the reactor body has a length of 35-95 cm, a width of 25-45 cm, and a depth of 2-10 cm.

Preferably, the depth of the culture solution in the reactor is 4-5 cm.

Preferably, the cross-sectional area of the reactor body is 900-2000 cm ² .

In some embodiments, the light sources are evenly distributed above the reactor body.

Preferably, the brightness of the light source is 2500-3500 lux.

In a second aspect, the present invention provides a salt-water Spirulina cultivation device, wherein the cultivation rack is a multi-layer cultivation rack, and at least one cultivation unit is placed on each layer of the cultivation rack; the number of reactor bodies can be adjusted as needed, and the reactor body, reactor top cover, light source, and salt-water Spirulina cultivation rack can be assembled to achieve large-scale cultivation of salt-water Spirulina.

In a third aspect, the present invention provides a method for cultivating Spirulina salizae using the Spirulina salizae cultivation unit or the Spirulina salizae cultivation device, comprising the following steps:

Adding monosodium glutamate wastewater with a volume fraction of 1‰-2‰ to seawater to prepare a culture medium solution; wherein the monosodium glutamate wastewater and seawater are filtered by 6 layers of gauze and 0.45 micron filter membrane respectively before use, the total nitrogen, total phosphorus and ammonia nitrogen of the monosodium glutamate wastewater are 55.26, 3.53 and 47.51 g/L, and the salinity of the seawater is 2.36%;

Add the culture medium solution into the reactor body to a depth of 4-5 cm, and add Spirulina salizawa thereto to an initial concentration of 0.05-0.15 g/L;

Turn on the light source for cultivation, with the initial illumination set at 2500-3500 lux. The carbon dioxide required and the released oxygen during the cultivation process are exchanged through the through holes on the top cover of the reactor;

Harvest after a set period of culture.

The beneficial effects achieved by one or more embodiments of the present invention are as follows:

(1) Through in-depth research on the effects of reactor culture medium depth and area on the growth of Spirulina, we optimized the design of a culture device suitable for its growth, making the culture conditions more in line with the growth requirements of Spirulina and significantly improving the culture efficiency.

(2) The culture device does not require traditional aeration, stirring and other power conditions and complex equipment operation. While maintaining the environment required for the growth of Spirulina saltwater, it reduces unnecessary energy consumption and reduces the operating cost of the culture;

(3) The structures of the reactor body, reactor cover, light source, and Spirulina culture rack are clear and concise, which reduces the difficulty of operation during the culture process; the reactor body is a rectangular box structure with a size that is easy to operate;

(4) The number of layers of the culture rack can be adjusted according to actual needs to meet the production needs of different scales, and it is also convenient for transportation and installation in different occasions.

(5) The multi-layer structure design of the culture device fully utilizes the space in the vertical direction, effectively reducing the floor space, and can be used for large-scale production within a limited space.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

FIG1 is a schematic structural diagram of a reactor body and a top cover according to an embodiment of the present invention;

FIG2 is a schematic structural diagram of a lighting unit above a reactor body according to an embodiment of the present invention;

FIG3 is a schematic diagram of the overall structure of the Spirulina culturing device of the present invention;

FIG4 is a comparison diagram of depth optimization of the culture solution of Spirulina salizawa according to an embodiment of the present invention;

FIG. 5 is a comparison diagram of cross-sectional area optimization of the Spirulina salizawa cultivation device according to an embodiment of the present invention.

In the figure: 1. Reactor body; 2. Reactor top cover; 3. Through hole; 4. Light source; 5. Fixer; 6. Connecting wire; 7. Culture rack; 8. Light controller.

DETAILED DESCRIPTION

It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used in the present invention have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

Example

A culture device for Spirulina salina, specifically as follows:

As shown in FIG1-3 , it includes a reactor body 1, a reactor top cover 2, and a salt-salt Spirulina culture rack 7;

The reactor body 1 adopts a box-type structure, and the reactor top cover 2 covers the reactor body 1 to avoid environmental pollution;

The reactor top cover 2 is provided with a through hole 3, and the same opening is used for feeding and gas exchange;

The spirulina culture rack 7 comprises a multi-layer structure, a light source 4 is embedded in the top of the space, and a reactor body 1 is placed at the bottom;

The light source 4 is fixed above each space of the spirulina culture rack 7 by a fixture 5, and different light sources are connected in series by connecting wires 6, and a light controller 8 controls the light source 4;

The reactor body 1 is made of plastic, and the top cover is a transparent film with 6 through holes; the reactor body 1 is 35-95 cm long, 25-45 cm wide, and 2-10 cm high; the fixture 5 for fixing the top light source is made of stainless steel; the spirulina culture rack is made of carbon steel, and each layer of the salt spirulina culture rack can be assembled with the light source, the reactor body, and the reactor top cover as needed.

When the reactor is used, the reactor body 1 is placed on the spirulina culture rack 7, microalgae inoculum and culture solution are added, and the reactor cover 2 is covered on the reactor body 1. The light source 4 is adjusted by the light controller 8 to set a suitable light scheme and parameters to start the microalgae culture process.

Optimize the culture parameters of the reactor:

The initial biomass concentration of Spirulina subsalsa was maintained at about 0.1g/L, the light intensity was 3000lux, the culture medium was seawater with 1‰ of MSG wastewater, the first harvest was carried out on the third day, and the second harvest was carried out on the sixth day after adding 2‰ of MSG wastewater. Among them, the MSG wastewater and seawater were filtered with 6 layers of gauze and 0.45 micron filter membrane respectively before use. The total nitrogen, total phosphorus and ammonia nitrogen of the MSG wastewater were 55.26, 3.53 and 47.51g/L, and the salinity of the seawater was 2.36%;

Three different depths of Spirulina culture medium: 2.25cm, 4.5cm, and 9cm;

Three different cross sections: length×width is 38×26=988 cm ² ; length×width is 71×28=1988 cm ² ; length×width is 93×43=3999 cm ² .

After selecting the most suitable cross-sectional area, the appropriate reactor body size is obtained. The biomass concentration is calculated according to the formula:

Wherein: DM ₃ is the weight of algal powder harvested on the third day; DM ₆ is the weight of algal powder harvested on the third day; V is the culture volume.

When the optimal depth of the culture solution in the reactor is 4.5 cm, the growth of Spirulina is the best, and the biomass concentration can reach 0.84 g/L; when the depth of the culture solution is 2.25 cm, the concentration of Spirulina can reach 0.75 g/L; when the depth of the culture solution is 9 cm, the concentration of Spirulina can reach 0.52 g/L, as shown in Figure 4. When the cross-sectional area of the reactor body is 988 ^cm2 and 1988 ^cm2 , the biomass concentration of Spirulina can reach 0.83 g/L and 0.82 g/L; when the cross-sectional area of the reactor body is 3999 ^cm2 , the biomass concentration of Spirulina can reach 0.72 g/L, as shown in Figure 5. Therefore, the appropriate depth and area of the culture solution in the reactor for cultivating Spirulina are 4.5 cm and 1988 ^cm2 , respectively.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims (6)

1. A spirulina culture unit, characterized in that: comprises a reactor main body, a reactor top cover and a culture rack, wherein,

The reactor main body is an incubator with an open top;

The reactor top cover is detachably covered on the top of the reactor main body, the reactor top cover is made of transparent materials, a plurality of through holes are formed in the reactor top cover, and carbon dioxide and released oxygen required in the culture process are exchanged through the through holes in the reactor top cover;

The light source is arranged on the culture rack and is positioned above the top cover of the reactor;

The length of the reactor main body is 35-95 cm, the width is 25-45 cm, and the depth is 2-10 cm;

The cross section area of the reactor main body is 900-2000 cm ², and the depth of the culture solution in the reactor is 4-5 cm;

the light sources are uniformly distributed above the reactor main body; the brightness of the light source is 3000 lux.

2. The spirulina culture unit of claim 1, wherein: two groups of through holes are formed in the reactor top cover and are oppositely arranged on two sides of the reactor top cover.

3. The spirulina culture unit according to claim 2, wherein: the number of the through holes in each group is 2-5, and the diameter of each through hole is 2-4 cm.

4. A device for culturing spirulina on salted soil is characterized in that: the culture rack is a multi-layer culture rack, and at least one culture unit according to any one of claims 1-3 is placed on each layer of culture rack.

5. A method for cultivating spirulina by using the spirulina cultivating unit of any one of claims 1 to 3 or the spirulina cultivating device of claim 4, characterized in that: the method comprises the following steps:

Adding monosodium glutamate wastewater with the volume fraction of 1-2%o into seawater to prepare a culture medium solution;

Adding the culture medium solution into the reactor main body to make the depth of the culture medium solution be 4-5 cm, and adding the spirulina with salt to make the initial concentration be 0.05-0.15 g/L;

turning on a light source to culture, setting initial illumination at 3000 lux, and exchanging carbon dioxide and released oxygen required in the culture process through a through hole on a top cover of the reactor;

harvesting after culturing for a set time.

6. The method for culturing spirulina platensis according to claim 5, wherein: before using, the monosodium glutamate wastewater and seawater are respectively filtered by adopting 4-7 layers of gauze and 0.45 micrometer filter membranes; the total nitrogen of the monosodium glutamate wastewater is 50-60 g/L, the total phosphorus is 3-4 g/L, and the ammonia nitrogen is 40-50 g/L.