CN118058281B

CN118058281B - Application of DMNT in preventing and controlling liriomyza sativae

Info

Publication number: CN118058281B
Application number: CN202410458515.XA
Authority: CN
Inventors: 周瀛; 陈秋霖; 祝增荣
Original assignee: Hainan Research Institute Of Zhejiang University
Current assignee: Hainan Research Institute Of Zhejiang University
Priority date: 2024-04-17
Filing date: 2024-04-17
Publication date: 2024-07-12
Anticipated expiration: 2044-04-17
Also published as: CN118058281A

Abstract

The invention provides an application of DMNT in preventing and controlling liriomyza sativae, belonging to the technical field of plant protection. The invention provides an application of DMNT in preventing and controlling liriomyza sativae, and the research shows that: DMNT with the concentration of 0.01-1 mug/mu L is sprayed on cowpea plants, so that the cowpea plants can simultaneously play roles in repelling liriomyza sativae and attracting natural enemy parasitic wasps, and can be used as a product for preventing and treating liriomyza sativae. Wherein, the composition can play a role in preventing and curing at the concentration of 0.01 mug/mu L, and can also reduce the cost of developing commodity.

Description

Application of DMNT in preventing and controlling liriomyza sativae

Technical Field

The invention relates to the technical field of plant protection, in particular to an application of DMNT in preventing and controlling liriomyza sativae.

Background

The leaf insect of the genus leaf insect of the subfamily liriomyza of the family diptera, the family liriomyza, is the leaf insect of cowpea, submerge the leaf insect of cowpea with the larva and take food harm, form obvious diving channel. The pest has strong fertility and serious generation overlapping, can reproduce in tropical regions all year round, can spread and spread rapidly, and the control method mainly comprises the steps of agricultural control of ploughing the land, killing overwintering larvae in surface soil or dead leaves and killing chemical pesticides. However, because the larvae have leaf-diving property, the pesticide is difficult to achieve a better control effect, and is extremely easy to explode and attack during the growth process of cowpea, the method becomes one of the important reasons for restricting the development of cowpea planting industry.

At present, the control of cowpea pests is seriously dependent on chemical pesticides, and excessive use of chemical pesticides is easy to induce high drug resistance of pests on one hand, so that the cowpea pests are rampant; on the other hand, the residual problem caused by pesticide use brings about hidden danger of food safety. Therefore, the development of new green ecological management technology for cowpea pests is urgent, and the development of an integrated control measure for the liriomyza sativae is urgently needed.

DMNT, (E) -4, 8-dimethyl-1, 3, 7-nonyltriene is an organic compound and has wide application in the fields of chemical industry, medicine, pesticide and the like. DMNTs have many good properties. Firstly, it has better solubility, and can be dissolved in solvents such as water, alcohols, ethers and the like. Secondly, DMNT has a low vapor pressure and is not volatile, so that the safety is high. In addition, the DMNT also has good thermal stability and chemical stability, is not easy to decompose and deteriorate, can be used as a solvent or an additive, and improves the solubility and stability of the product.

Disclosure of Invention

The invention aims to develop a green prevention and treatment measure for liriomyza sativae.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides an application of DMNT in preventing and controlling liriomyza sativae.

Preferably, the control is achieved by repelling the liriomyza sativae and/or attracting natural enemies of the liriomyza sativae.

Preferably, the natural enemy of the liriomyza sativae is a hornet.

Preferably, the concentration of the DMNT is 0.01-100 mug/mu L.

Preferably, the concentration of the DMNT is 0.01-1 mug/mu L.

Preferably, the spraying amount of the DMNT is 5-10 mL/plant cowpea.

Advantageous effects

The invention is found through experiments: the cowpea plant volatile DMNT with the concentration of 0.01-1 mug/mu L is sprayed on cowpea plants, so that the cowpea plant volatile DMNT can simultaneously play roles in repelling liriomyza sativae and attracting natural enemy parasitic bees, and can be used as a product for preventing and treating liriomyza sativae. Wherein, the composition can not only play a role in prevention and treatment but also reduce the cost of developing commodity at the concentration of 0.01 mug/mug.

Drawings

FIG. 1 is a schematic diagram of a Y-tube test.

FIG. 2 shows the selectivity of the Spot fly of example 1 for DMNT solutions of different concentrations.

FIG. 3 shows the selectivity of parasitic wasps of example 1 to 0.01. Mu.g/. Mu.L DMNT solution.

FIG. 4 shows the selectivity of the leaf miner of example 2 for plants sprayed with 1. Mu.g/. Mu.L of DMNT.

FIG. 5 shows the selectivity of the leaf miner of example 2 for plants sprayed with 0.1. Mu.g/. Mu.L DMNT.

FIG. 6 shows the selectivity of the leaf miner of example 2 for plants sprayed with 0.01. Mu.g/. Mu.L DMNT.

FIG. 7 shows the selectivity of parasitic wasps of example 2 for plants sprayed with DMNT at 0.01. Mu.g/. Mu.L.

Detailed Description

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

Example 1

DMNT were prepared in 100. Mu.g/. Mu.L, 10. Mu.g/. Mu.L, 1. Mu.g/. Mu.L, 0.1. Mu.g/. Mu.L, 0.01. Mu.g/. Mu.L solutions with 5% ethanol, respectively, and placed in sealable glass jars with controls of 5% ethanol.

A first group: different concentrations of DMNT solution and 5% ethanol were separately attached to two arms of a Y-tube (as shown in fig. 1), and adult leaf spot flies were placed at the bottom of the Y-tube, and the selectivity of leaf spot flies for different concentrations of DMNT was evaluated using the Y-tube.

Second group: the DMNT solution with different concentrations and 5% ethanol are respectively connected to two arms of a Y-shaped tube, the natural enemy of the liriomyza sativae, the chrysomyia pupae and the chrysomyia colleague are placed at the bottom of the Y-shaped tube, and the selectivity of the natural enemy of the liriomyza sativae, the chrysomyia pupae and the chrysomyia colleague, to the DMNT is evaluated by utilizing the Y-shaped tube.

The results show that the liriomyza sativae has the repellency to DMNT with different concentrations (figure 2), and the DMNT with the concentration of 0.01 mug/mug has better attraction effect to the fly pupae gilt bee (figure 3). This shows that DMNT can be applied to the prevention and treatment of the liriomyza sativae, can not only play a role in repelling the liriomyza sativae, but also can attract natural enemy parasitic wasps.

Example 2

DMNT is prepared into solutions with the concentration of 1 mug/mu L, 0.1 mug/mu L and 0.01 mug/mu L respectively by using 5% ethanol, cowpea plants are sprayed respectively, 5 mL per cowpea plants are sprayed, cowpea plants sprayed with different concentrations of DMNT solution and 5% ethanol are respectively connected to two arms of a Y-shaped tube, adult liriomyza sativae is placed at the bottom of the Y-shaped tube, and the selectivity of the liriomyza sativae on the sprayed DMNT plants and control plants is compared through the Y-shaped tube. It was found that DMNT concentrations of 1. Mu.g/. Mu.L (FIG. 4), 0.1. Mu.g/. Mu.L (FIG. 5), 0.01. Mu.g/. Mu.L (FIG. 6) were all able to significantly reduce the selectivity of Spot-fly to cowpea plants.

The cowpea plants sprayed with 0.01 mug/mug of DMNT solution and 5% ethanol are respectively connected to two arms of a Y-shaped pipe, the natural enemy of the liriomyza sativae, the chrysomyia maculata, is placed at the bottom of the Y-shaped pipe, and the selectivity of the natural enemy of the liriomyza sativae, the chrysomyia maculata, to the cowpea plants sprayed with the DMNT is evaluated by utilizing the Y-shaped pipe. DMNT at a concentration of 0.01 μg/μl was found to be significantly attractive to natural enemy parasitic bees (fig. 7).

In summary, when the cowpea plant volatile DMNT is sprayed on cowpea plants at a low concentration of 0.01-1 mug/mug, the cowpea plant volatile DMNT can repel spot liriomyza and attract natural enemy parasitic wasps. Wherein, the composition can not only play a role in prevention and treatment but also reduce the cost of developing commodity under the concentration of 0.01 mug/mug.

The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims

1. (E) -4, 8-dimethyl-1, 3, 7-nonyltriene for combating liriomyza sativae;

The prevention and control are realized by repelling the liriomyza sativae;

the concentration of the (E) -4, 8-dimethyl-1, 3, 7-nonyltriene is 0.01-1 mug/mu L.

2. The use according to claim 1, wherein the spray amount of (E) -4, 8-dimethyl-1, 3, 7-nonyltriene is 5 to 10 mL/plant cowpea.