CN118050209A - Portable suction filtration kit for water quality microorganism detection - Google Patents

Abstract

The application discloses a portable suction filtration kit for water quality microorganism detection, which comprises the following components: the sampling bottle is provided with a sampling port communicated with the internal cavity, and the sampling port is provided with a sampling connecting part; the suction device comprises a suction pipe with a suction effect, and the suction pipe is vertically arranged; the device comprises a detection tube, a filter membrane and a filter membrane, wherein one end surface of the detection tube is provided with a first inlet, the other end surface of the detection tube is provided with a second outlet, and the filter membrane is arranged between the first inlet and the second outlet; the pipe wall of the first inlet end of the detection pipe is detachably connected and matched with the sampling connecting part, and the pipe wall of the second outlet end of the detection pipe is detachably connected and matched with the suction pipe; after the sampling bottle collects water samples, the first inlet is connected with the sampling port, the combination body formed by the sampling bottle and the detection tube is vertically arranged, the second outlet is connected with the suction tube, the water samples in the sampling bottle enter the suction device through the detection tube under the action of gravity and suction of the suction device, and microorganisms in the water samples are reserved in the detection tube under the action of filtration of the filter membrane.

Description

A portable filtration kit for water quality microbial detection

Technical Field

The present application belongs to the technical field of water quality detection, and specifically relates to a portable filtration kit for water quality microorganism detection.

Background technique

The WHO drinking water guidelines have confirmed that most of the health problems related to drinking water come from microorganisms. However, there are many types of pathogenic microorganisms in water, and it is impossible to test them one by one. Generally, a representative type or class of microorganisms is selected as indicator bacteria, that is, conventional microbial indicators of water quality are used to determine whether the water body is contaminated by microorganisms, especially intestinal pathogenic microorganisms. Water quality microbial indicators are important indicators for evaluating the biological safety of drinking water. The safety of water quality can be determined by testing conventional microbial indicators of water quality.

Routine microbial indicators of water quality include total colony count, total coliform group and Escherichia coli. The total colony count is an indicator for evaluating the cleanliness of water quality and assessing the purification effect. It indicates the degree of bacterial contamination of water quality. Generally speaking, the more bacteria there are, the higher the degree of contamination. Total coliform group is not a certain type or genus of bacteria, but a group of bacteria with common characteristics. They are indicator bacteria of fecal contamination. The detection of total coliform group often indicates that the water quality is contaminated by feces and there is a possibility of transmission of intestinal infectious diseases. Escherichia coli is the most meaningful indicator bacteria of fecal contamination. If Escherichia coli is detected in the water sample, it means that the water body may have been contaminated by feces and there is a possibility of intestinal infectious diseases. Corresponding measures must be taken.

The "Sanitary Standard for Drinking Water" stipulates the sanitary requirements for drinking water quality, the sanitary requirements for drinking water sources, the sanitary requirements for centralized water supply units, the sanitary requirements for secondary water supply, the sanitary requirements for drinking water sanitation and safety products, and the water quality monitoring and water quality inspection methods. This standard applies to all types of centralized drinking water supply in urban and rural areas, and also to decentralized drinking water supply.

Since the source of drinking water is relatively vast, water samples need to be collected from multiple locations in the water source. The water sources are far apart and are scattered. After collecting the water samples, the water samples are taken back to the laboratory for testing. This method cannot effectively utilize the time spent on the journey, and the microbial culture involved in water quality testing takes a long time. Therefore, the whole process takes a long time to obtain the results. In addition, more water samples will be collected during water quality sampling, and the heavy weight of the water samples is a burden for the mobile staff.

Most water sources are located in the wild, and most of them do not have hardened roads leading directly to the water sources. At this time, staff are required to carry equipment and walk to the water source. Therefore, the portability of the equipment directly affects the difficulty of sampling for staff.

When using a container to collect water samples, you should first use the water sample to wash the container, and avoid getting your fingers on the container mouth to avoid contaminating the container.

When sampling drinking water, the water content in the water sample can be reduced to increase the proportion of microorganisms in the water sample. By concentrating the collected water samples, it is easier to culture the microorganisms in the water samples at a later time, shorten the culture time, and facilitate the determination of the total colony count, total coliform group and Escherichia coli in the water samples to obtain more accurate results.

Therefore, a portable suction device that is easy to operate is needed to increase the proportion of microorganisms in water samples while preventing external pollutants from contaminating water samples due to operations such as pipeline connections. In addition, the suction filtration kit should have a certain degree of portability to meet the needs of staff.

Summary of the invention

In order to facilitate the concentration of water samples, reduce the labor intensity of staff responsible for water sample collection, improve the portability of filtration kits for routine microbial testing of water quality, and reduce the possibility of external pollutants contaminating water samples during the water concentration process, the present application provides a portable filtration kit for water quality microbial testing.

A portable filtration kit for water quality microorganism detection, comprising: a sampling container, the sampling container is provided with a sampling port connected to an internal cavity, the sampling port is provided with a sampling connection part; a suction device, the suction device comprises a suction tube with a suction function, the suction tube is vertically arranged; a detection tube, one end face of the detection tube is provided with a first inlet, the other end face is provided with a second outlet, and a filter membrane is provided in the detection tube between the first inlet and the second outlet; the tube wall of the first inlet end of the detection tube is detachably connected and matched with the sampling connection part, and the tube wall of the second outlet end is detachably connected and matched with the suction tube; after the sampling container collects the water sample, the first inlet is connected to the sampling port, the combination of the sampling container and the detection tube is vertically arranged, and the second outlet is connected to the suction tube, under the suction action of gravity and the suction device, the water sample in the sampling container enters the suction device through the detection tube, and the microorganisms in the water sample are retained in the detection tube under the filtering action of the filter membrane.

The inspectors can carry three pieces of equipment, namely, a sampling container, a detection tube and a suction device, to go to the water source to collect water samples. There is no need to carry connecting pipes or adapters. Water samples can be collected using a sampling container, and then the sampling port is set upward, and the first inlet of the detection tube is connected to the sampling port, and then the combination of the sampling container and the detection tube formed by a detachable connection is flipped so that the second outlet is set downward, and then the second outlet is connected to the vertically arranged suction tube, so that the combination of the sampling container and the detection tube is vertically arranged and connected to the suction device, so that the suction device sucks the water sample in the sampling container through the detection tube. Under the influence of gravity and the suction action of the suction device, the water sample in the sampling container enters the detection tube, and the filter membrane in the detection tube retains the microorganisms in the water sample in the detection tube. As the water in the sampling container enters the suction tube, the amount of water in the sampling container and the detection tube is continuously reduced. Finally, the water sample is concentrated in the detection tube, which is convenient for subsequent operations. The present application is easy to carry and install, and is suitable for inspectors to work in the field.

Since the filter membrane has a certain barrier effect, during the installation of the sampling container and the detection tube, the filter membrane can prevent external pollutants from entering the sampling container.

In one embodiment of the present application, the diameter of the second outlet is smaller than the inner diameter of the detection tube.

In order to make the suction device portable, the power of the suction pump of the suction device is limited, so that the suction force that the suction pump of the suction device can generate is limited. By making the diameter of the second outlet smaller than the inner diameter of the detection tube, the suction effect of the suction device on the detection tube is ensured. At the same time, the possibility of external pollutants entering the detection tube through the second outlet is reduced.

In one embodiment of the present application, the filter membrane and the second outlet are spaced apart along the axial direction of the detection tube, and the filter membrane and the inner wall of the detection tube form a filtration cavity, which is located between the filter membrane and the second outlet and is connected to the second outlet; the liquid entering the detection tube through the first inlet, after being filtered by the filter membrane, leaves the detection tube through the filtration cavity and the second outlet in turn.

In order to ensure the filtering effect of the filter membrane on the water sample and improve the utilization rate of the filter membrane, when the filter membrane covers the flow cross-section of the detection tube, since the diameter of the second outlet is smaller than the inner diameter of the detection tube, the projection of the second outlet on the filter membrane cannot completely cover the filter membrane. The second outlet is connected to the filtration cavity. When the suction tube sucks the filtration cavity through the second outlet, there is a negative pressure in the entire filtration cavity, so that the suction tube sucks the entire filter membrane through the second outlet and the filtration cavity, thereby improving the suction effect.

In one embodiment of the present application, a support plate is provided in the detection tube; the support plate is provided between the filter membrane and the second outlet, and the filter membrane is provided on the end face of the support plate close to the first inlet; the support plate is connected to the tube wall of the detection tube, and a support through hole is provided on the end face of the support plate; the liquid entering the detection tube through the first inlet, after being filtered by the filter membrane, leaves the detection tube through the support through hole and the second outlet in turn.

During the suction operation, the filter membrane is subjected to a force in the direction close to the second outlet under the action of the suction device, and the support plate can provide support for the filter membrane to reduce the deformation of the filter membrane and prevent the filter membrane from rupturing. The support through-holes that penetrate the two end surfaces of the support plate are arranged on the end surface of the support plate. The water sample that enters the detection tube through the first inlet passes through the filter membrane, the support through-holes, and the second outlet in turn, leaving the detection tube and entering the suction device.

In one embodiment of the present application, a first thread is provided on the tube wall of the first inlet end of the detection tube; the sampling connection portion is a second thread, and the second thread is threadably connected and matched with the first thread.

The detection tube and the sampling container are connected by threads, which can effectively improve the stability of the connection between the sampling container and the detection tube, avoid separation of the sampling container and the detection tube during the installation process, cause water sample leakage, and avoid unnecessary repetitive work for the staff.

In one embodiment of the present application, the end face of the second outlet end of the detection tube is connected to a connecting straight tube, the axial direction of the connecting straight tube is the same as the axial direction of the detection tube, and the connecting straight tube is connected to the second outlet; the connecting straight tube is plugged into the suction tube, and the suction tube can be connected to the detection tube through the connecting straight tube.

The speed of installing the combination of the sampling container and the detection tube to the suction device can be greatly improved by plugging and matching the detection tube with the suction tube.

In one embodiment of the present application, the end face of the second outlet end of the detection tube is also connected to a fixed tube, the inner diameter of the fixed tube is larger than the outer diameter of the connecting straight tube, the connecting straight tube is arranged in the fixed tube, and the second outlet, the connecting straight tube and the fixed tube are coaxially arranged; the outer diameter of the suction tube is not larger than the inner diameter of the fixed tube, the inner diameter of the suction tube is not smaller than the outer diameter of the connecting straight tube, and the connecting straight tube can be inserted into the suction tube and plug-fitted with the suction tube.

In one embodiment of the present application, the suction device also includes a mounting tube, which forms a vertically arranged accommodating hole, and the diameter of the accommodating hole is not less than the diameter of the detection tube; a suction tube is arranged in the accommodating hole, and the accommodating hole is coaxially arranged with the suction tube; the detection tube can be inserted into the accommodating hole, and the inner wall of the accommodating hole limits the radial movement of the detection tube.

In one embodiment of the present application, the filtering device also includes a main body shell and a support frame detachably connected to the main body shell; the combination composed of the sampling container and the detection tube is vertically arranged, and when connected to the suction tube through the second outlet, the support frame limits the movement of the sampling container and the detection tube.

A method for using a portable suction filtration kit for water quality microorganism detection, the portable suction filtration kit for water quality conventional microorganism detection comprises a sampling container provided with a sampling port; and a detection tube provided with a first inlet at one end and a second outlet at the other end, the detection tube is provided with a filter membrane between the first inlet and the second outlet, and a suction device provided with a suction tube, the suction tube is vertically arranged;

Usage methods include:

S1: Collect water samples using sampling containers;

S2: The sampling port of the sampling container is set upward;

S3: providing a force so that the first inlet of the detection tube is disposed downward;

S4: The first inlet arranged downward is detachably connected to the sampling port arranged upward;

S5: providing a force to rotate the assembly consisting of the sampling container and the detection tube so that the second outlet of the detection tube faces downward;

S6: The second outlet facing downward is detachably connected to the suction tube, and the suction device sucks the combination of the sampling container and the detection tube through the suction tube, and the microorganisms in the water sample are retained on the filter membrane of the detection tube.

The technical solution recorded in this application has at least the following beneficial effects:

1. The inspector can carry three pieces of equipment, namely, a sampling container, a detection tube and a suction device, to go to the water source to collect water samples. There is no need to carry connecting pipes or adapters. Water samples can be collected using a sampling container, and then the sampling port is set upward, and the first inlet of the detection tube is connected to the sampling port, and then the combination of the sampling container and the detection tube formed by a detachable connection is turned over, so that the second outlet is set downward, and then the second outlet is connected to the vertically arranged suction tube, so that the combination of the sampling container and the detection tube is vertically arranged and connected to the suction device, so that the suction device sucks the water sample in the sampling container through the detection tube. Under the influence of gravity and the suction action of the suction device, the water sample in the sampling container enters the detection tube, and the filter membrane in the detection tube retains the microorganisms in the water sample in the detection tube. As the water in the sampling container enters the suction tube, the amount of water in the sampling container and the detection tube is continuously reduced. Finally, the water sample is concentrated in the detection tube, which is convenient for subsequent operations. The present application is easy to carry and install, and is suitable for inspectors to work in the field.

2. Since the filter membrane has a certain barrier effect, during the installation of the sampling container and the detection tube, the filter membrane can prevent external pollutants from entering the sampling container.

3. The installation tube and support frame described in this application ensure the stability of the connection between the combination of the sampling container and the detection tube and the filtration device, avoiding the shaking of the combination of the sampling container and the detection tube and the occurrence of air leakage during the suction operation. The installation tube and support frame improve the reliability of the device when used outdoors.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic structural diagram of an exemplary embodiment of the present application;

FIG2 is a schematic structural diagram of a schematic implementation of the suction tube in the present application;

FIG3 is a schematic structural diagram of an exemplary implementation of the second outlet in the present application;

FIG4 is a schematic structural diagram of a schematic implementation of a connecting straight pipe in the present application;

FIG5 is a schematic structural diagram of a schematic implementation of a support plate in the present application;

FIG6 is a schematic structural diagram of a schematic implementation of a mounting tube in the present application;

FIG7 is a schematic structural diagram of an exemplary embodiment of the size matching between the suction pipe and the mounting pipe in the present application;

FIG8 is a schematic structural diagram of an exemplary embodiment of a threaded cap in the present application;

FIG. 9 is a schematic structural diagram of a schematic implementation of a support frame in the present application.

In the figure:

101. sampling bottle; 102. sampling port;

201, suction device; 202, suction pipe; 203, drainage pipe; 204, installation pipe; 205, main machine housing; 206, threaded connection hole;

301, detection tube; 302, first inlet; 303, second outlet; 304, filter membrane; 305, connecting straight tube; 306, fixed tube; 307, filtration cavity; 308, support plate; 309, support through hole; 310, support ring; 311, threaded cap; 312, plug; 401, support frame; 402, telescopic rod; 403, support plate.

Detailed ways

In order to have a clearer understanding of the technical features, purposes and effects of the present application, the specific implementation methods of the present application are now described with reference to the accompanying drawings. The same reference numerals in the drawings represent components with the same structure or similar structures but the same functions.

In this document, “exemplary” means “serving as an example, instance or illustration”, and any diagram or implementation described in this document as “exemplary” should not be interpreted as a more preferred or more advantageous technical solution.

In order to simplify the drawings, only the parts related to the present application are schematically shown in each figure, and they do not represent the actual structure of the product. In addition, in order to simplify the drawings and facilitate understanding, in some figures, only one of the parts with the same structure or function is schematically drawn or marked.

Please refer to Figures 1 to 9 for an understanding of this application.

Referring to Figures 1 and 2, the portable filtration kit for routine microbial testing of water quality includes a suction device 201, which includes a suction tube 202 with a suction function, and the suction tube 202 is vertically arranged; in one embodiment of the present application, the suction device 201 also includes a suction pump and a drain pipe 203, and the suction pump is connected to the suction tube 202. When the suction pump is working, a negative pressure can be generated in the suction tube 202, thereby generating a suction effect, and the water sample entering the suction pump can enter the outside through the drain pipe 203 under the action of the suction pump.

Referring to Figures 1 and 2, the portable filtration kit for routine microbial detection of water quality also includes a sampling bottle 101 and a detection tube 301. The sampling bottle 101 is provided with a sampling port 102 connected to the internal cavity, and the sampling port 102 is provided with a sampling connection portion; referring to Figure 3, the detection tube 301, one end face of the detection tube 301 is provided with a first inlet 302, and the other end face is provided with a second outlet 303, and a filter membrane 304 is provided in the detection tube 301 between the first inlet 302 and the second outlet 303; the end of the detection tube 301 having the first inlet 302 is the first inlet 302 end, and the end of the detection tube 301 having the second outlet 303 is the second outlet 303 end, the tube wall of the first inlet 302 end of the detection tube 301 is detachably connected and matched with the sampling connection portion, and the tube wall of the second outlet 303 end is detachably connected and matched with the suction tube 202, such as the tube wall of the second outlet 303 end is plugged and matched with the suction tube 202.

In one embodiment of the present application, a sampling connection portion is provided on the inner wall of the sampling port 102 of the sampling bottle 101, and the sampling connection portion is a second thread. A first thread that can be threadedly connected to the second thread is provided on the outer tube wall of the first inlet 302 end of the detection tube 301, so that the detection tube 301 is connected to the sampling bottle 101 by a threaded connection.

After the sampling bottle 101 collects the water sample, the sampling port 102 is set upward to prevent the water sample in the sampling bottle 101 from flowing out of the sampling port 102 under the action of gravity, and then the detection tube 301 and the sampling bottle 101 are threadedly connected so that the first inlet 302 is connected to the sampling port 102, and then the combination of the sampling bottle 101 and the detection tube 301 is turned over. The combination of the sampling bottle 101 and the detection tube 301 is set vertically, and the detection tube 301 is connected to the suction tube 202, and the second outlet 303 is connected to the suction tube 202. Under the action of gravity and the suction of the suction device 201, the water sample in the sampling bottle 101 enters the suction device 201 through the detection tube 301, and the microorganisms in the water sample are retained in the detection tube 301 under the filtering action of the filter membrane 304. After the suction operation is completed, the detection tube 301 is disconnected from the sampling bottle 101 and the suction tube 202, and the first inlet 302 and the second outlet 303 are blocked using sealing parts, such as using a threaded cap 311 to block the first inlet 302 and using a plug 312 to block the second outlet 303, see Figure 8, so that the water sample is concentrated in the detection tube 301, and the detection device can be used to detect the water sample in the detection tube 301.

The body of the sampling bottle 101 can be a bottle body with a certain flexibility, or the body of the sampling bottle 101 has a foldable bottle wall, or the body of the sampling bottle 101 is a hard shell, the internal cavity of the sampling bottle 101 is connected to the outside through an air pipeline, and a one-way valve is provided on the air pipeline. The one-way valve on the air pipeline limits the water sample of the sampling bottle 101 from entering the outside through the air pipeline. The air pipeline is also provided with a filter, and the outside air can enter the sampling bottle 101 after being filtered by the filter. The water sample continuously leaves the sampling bottle 101 under the suction action of the suction device 201 and enters the detection tube 301. The air can enter the sampling bottle 101 through the air pipeline to adjust the air pressure in the sampling bottle 101 so that the water sample in the sampling bottle 101 can leave the sampling bottle 101 as much as possible. Of course, technicians in the technical field to which the present application belongs can understand that the sampling container used in the portable filtration kit for routine microbial detection of water quality can also be a sampling bag, and the flexible bag wall of the sampling bag can adapt to the change in the volume of the water sample in the sampling bag.

The technicians in the technical field of the present application can understand that the arrangement position of the first thread and the second thread in the present application is not limited to the method of setting the first thread on the outer tube wall at the first inlet 302 end and setting the second thread on the inner wall of the sampling port 102. It can also be that the first thread is set on the inner tube wall of the first inlet 302 and the second thread is set on the outer bottle wall at the sampling port 102, so that the detection tube 301 is threadedly connected with the sampling bottle 101. Of course, the connection method between the detection tube 301 and the sampling bottle 101 is not limited to the threaded connection method, and can also have other detachable The connection is realized by, for example, plugging and matching the detection tube 301 with the sampling bottle 101, the outer diameter of the detection tube 301 is smaller than the diameter of the sampling port 102, and the sampling port 102 and the detection tube 301 are plugged and matched, thereby realizing the connection between the detection tube 301 and the sampling bottle 101. Of course, the detachable connection between the sampling bottle 101 and the detection tube 301 can also be realized by other methods, such as snap-on connection, which will not be elaborated here, and it is sufficient to ensure that the sampling bottle 101 and the detection tube 301 can establish a tight detachable connection relationship while ensuring the airtightness of the connection between the sampling bottle 101 and the detection tube 301.

Those skilled in the art to which the present application belongs will appreciate that there are a variety of implementation methods for achieving plug-in connection between the detection tube 301 and the suction tube 202 , which are described in detail below.

In one embodiment of the present application, the second outlet 303 of the detection tube 301 is not smaller than the diameter of the suction tube 202, and the suction tube 202 can be inserted into the second outlet 303. The airtightness of the connection between the second outlet 303 and the suction tube 202 can be ensured by providing rubber on the inner wall of the second outlet 303, or by interference fit between the second outlet 303 and the outer wall of the suction tube 202, so as to avoid air leakage at the connection between the suction tube 202 and the second outlet 303 during the suction operation.

Of course, there are other ways to realize the plug-in connection between the detection tube 301 and the suction tube 202 .

Referring to Fig. 1, Fig. 2, Fig. 4, and Fig. 5, in one embodiment of the present application, a connecting straight tube 305 is connected to the end surface of the second outlet 303 of the detection tube 301, the axial direction of the connecting straight tube 305 is the same as the axial direction of the detection tube 301, and the connecting straight tube 305 is connected to the second outlet 303; the connecting straight tube 305 is plugged and matched with the suction tube 202, and the suction tube 202 can be connected with the detection tube 301 through the connecting straight tube 305. The outer diameter of the suction tube 202 is not greater than the inner diameter of the connecting straight tube 305, and the suction tube 202 can be inserted into the connecting straight tube 305, so that the suction tube 202 and the connecting straight tube 305 are plugged and matched.

Of course, the size matching relationship between the suction tube 202 and the connecting straight tube 305 is not limited to the size matching relationship that the outer diameter of the suction tube 202 is not greater than the inner diameter of the connecting straight tube 305, but it can also be that the inner diameter of the suction tube 202 is not less than the outer diameter of the connecting straight tube 305, and the connecting straight tube 305 can be inserted into the suction tube 202 and plugged into the suction tube 202.

The plug-in matching mode of the connecting straight tube 305 and the suction tube 202 can significantly increase the contact area between the connecting straight tube 305 and the suction tube 202, thereby ensuring the stability of the connection between the combination of the sampling bottle 101 and the detection tube 301 and the suction tube 202, and avoiding the leakage at the connection between the detection tube 301 and the suction tube 202. At the same time, since the combination of the sampling bottle 101 and the detection tube 301 is arranged vertically, under the influence of gravity, the detection tube 301 and the suction tube 202 may shake, and the detection tube 301 and the suction tube 202 are plugged in the connecting straight tube 305. The connection stability can be significantly improved, and at the same time, it is easy to install, and the reliability of the connection between the detection tube 301 and the suction tube 202 is improved, which provides protection for the staff working in the field. At the same time, since the connecting straight tube 305 and the detection tube 301 can be connected in an integrated manner, the number of parts that the staff needs to carry is reduced, thereby improving the portability of the kit, and the staff can also carry the filtration kit more conveniently when hiking in the field.

Referring to Figs. 1, 2, 4 and 5, further, the end surface of the second outlet 303 of the detection tube 301 is also connected to a fixed tube 306, the inner diameter of the fixed tube 306 is larger than the outer diameter of the connecting straight tube 305, the connecting straight tube 305 is arranged in the fixed tube 306, the second outlet 303, the connecting straight tube 305 and the fixed tube 306 are coaxially arranged; the outer diameter of the suction tube 202 is not larger than the inner diameter of the fixed tube 306, the inner diameter of the suction tube 202 is not smaller than the outer diameter of the connecting straight tube 305, the connecting straight tube 305 can be inserted into the suction tube 202, and the suction tube 202 is connected to the suction tube 202. The tube 202 is plugged in, so when the suction tube 202 is plugged in with the connecting straight tube 305, along the radial direction of the connecting straight tube 305, the suction tube 202 is located between the fixed tube 306 and the connecting straight tube 305. This arrangement can significantly improve the stability of the connection between the suction tube 202 and the detection tube 301. At the same time, the inner wall of the suction tube 202 can be connected to the outer wall of the connecting straight tube 305, and the outer wall of the suction tube 202 is connected to the inner wall of the fixed tube 306. This arrangement reduces the possibility of air leakage, thereby improving the working stability of the filtration kit.

Since the suction tube 202 is located between the fixed tube 306 and the connecting straight tube 305, the fixed tube 306 and the connecting straight tube 305 can limit the radial movement of the suction tube 202 along the detection tube 301. At the same time, when the end surface of the second outlet 303 of the detection tube 301 only has the connecting straight tube 305, the connecting straight tube 305 needs to withstand a large torque and force to ensure the stability of the combination composed of the sampling bottle 101 and the detection tube 301 when it is vertically arranged. The fixed tube 306 and the connecting straight tube 305 can both apply force to the suction tube 202. Since the inner diameter of the fixed tube 306 is larger than that of the suction tube 202, The outer diameter of the suction tube 202 is large, so that the suction tube 202 can give a larger torque to the fixed tube 306. When the end face of the second outlet 303 of the detection tube 301 only has a connecting straight tube 305, the suction tube 202 can give a larger torque to the combination of the sampling bottle 101 and the detection tube 301 through the fixed tube 306 and the connecting straight tube 305, thereby avoiding damage to the connecting straight tube 305 and avoiding the sampling bottle 101 and the detection tube 301 from being skewed when the combination of the sampling bottle 101 and the detection tube 301 is connected to the suction tube 202 through the connecting straight tube 305.

Technicians in the technical field to which the present application belongs can understand that the outer diameter of the suction tube 202 can be equal to the inner diameter of the fixed tube 306, and the inner diameter of the suction tube 202 can be equal to the outer diameter of the connecting straight tube 305, thereby ensuring the airtightness of the connection between the suction tube 202 and the fixed tube 306. Of course, the outer diameter of the suction tube 202 can be smaller than the inner diameter of the fixed tube 306, and the inner diameter of the suction tube 202 can be larger than the outer diameter of the connecting straight tube 305. The suction tube 202 is plugged in with the fixed tube 306 and the connecting straight tube 305 through components such as rubber sealing bushings to ensure the airtightness between the suction tube 202 and the fixed tube 306 and the connecting straight tube 305. Of course, there are other ways to ensure the airtightness of the connection between the suction tube 202 and the fixed tube 306 and the connecting straight tube 305, which will not be repeated here.

Technicians in the technical field to which the present application belongs can understand that the implementation method of connecting the detection tube 301 and the suction tube 202 in the present application is not limited to the method of connecting the detection tube 301 and the suction tube 202 by plug-in connection, and the detection tube 301 and the suction tube 202 can also be connected by threaded connection, such as the outer wall of the connecting straight tube 305 is provided with an external thread, the inner wall of the suction tube 202 is provided with an internal thread, and the connecting straight tube 305 is threadedly connected to the suction tube 202, or the second outlet 303 is provided with an internal thread, the outer wall of the suction tube 202 is provided with an external thread, and the second outlet 303 is threadedly connected to the suction tube 202.

In one embodiment of the present application, the diameter of the second outlet 303 is smaller than the inner diameter of the detection tube 301. Technicians in the technical field to which the present application belongs can understand that the inner diameter of the connecting straight tube 305 in the present application can be equal to the diameter of the second outlet 303 end, and the outer diameter of the connecting straight tube 305 is smaller than the diameter of the detection tube 301, thereby improving the portability of the filtration kit.

Referring to Figures 1, 6 and 7, in one embodiment of the present application, the suction device 201 also includes a mounting tube 204, which forms a vertically arranged accommodating hole, and the diameter of the accommodating hole is not less than the diameter of the detection tube 301; a suction tube 202 is arranged in the accommodating hole, and the accommodating hole and the suction tube 202 are coaxially arranged; the detection tube 301 can be inserted into the accommodating hole, and the connecting straight tube 305 is plugged into the suction tube 202, and the inner wall of the accommodating hole limits the radial movement of the detection tube 301, so that the presence of the mounting tube 204 can prevent the combination of the sampling bottle 101 and the detection tube 301 from being skewed, which is more suitable for sampling work in the field.

Referring to Figure 9, in one embodiment of the present application, the filtering device also includes a main housing 205 and a support frame 401 detachably connected to the main housing 205; the combination composed of the sampling bottle 101 and the detection tube 301 is vertically arranged, and when connected to the suction tube 202 through the second outlet 303, the support frame 401 limits the movement of the sampling bottle 101 and the detection tube 301.

The body of the sampling bottle 101 is a hard shell, the main body shell 205 is provided with a threaded connection hole 206, the support frame 401 includes a support plate 403 and a telescopic rod 402, one end of the telescopic rod 402 is connected to the support plate 403, and the other end is provided with an external thread that can cooperate with the threaded connection hole 206, the telescopic rod 402 is perpendicular to the support plate 403, when the detection tube 301 is connected to the suction tube 202, the support plate 403 abuts against the bottom of the sampling bottle 101, and the telescopic rod 402 is threadedly connected to the threaded connection hole 206, thereby fixing the combination of the sampling bottle 101 and the detection tube 301 to prevent the detection tube 301 from detaching from the suction tube 202, and the telescopic rod 402 can be telescoped for easy outdoor carrying.

Technicians in the technical field to which the present application belongs can understand that the body of the sampling bottle 101 is a hard shell, and the sampling bottle 101 can be provided with an air pipeline. The air pipeline is provided with a one-way valve to limit the liquid in the sampling bottle 101 from entering the outside through the air pipeline. The air pipeline is provided with a filter. When the suction device 201 is suctioning, outside air can enter the sampling bottle 101 through the air pipeline.

Technicians in the technical field to which the present application belongs can understand that the length of the support frame 401 in the present application can be the same as the length of the combination consisting of the sampling bottle 101 and the detection tube 301, so that when the combination consisting of the sampling bottle 101 and the detection tube 301 is connected to the suction tube 202, the support frame 401 is connected to the main body housing 205, so that the support frame 401 fixes the combination consisting of the sampling bottle 101 and the detection tube 301 to the main body housing 205, thereby avoiding the detection tube 301 from being skewed or insufficiently fixed when the suction tube 202 is used to suction the combination consisting of the sampling bottle 101 and the detection tube 301, resulting in air leakage between the detection tube 301 and the suction tube 202.

Technicians in the technical field to which the present application belongs can understand that the shape of the support frame 401 in the present application can match the shape of the combination consisting of the sampling bottle 101 and the detection tube 301, and there are many ways to implement the detachable connection between the support frame 401 and the main housing 205, such as the support frame 401 includes a support plate 403 and a connecting column, the connecting column is connected to the support plate 403 and can rotate around its own axis, one end of the connecting column can be threadedly connected to the main housing 205, so that when the combination consisting of the sampling bottle 101 and the detection tube 301 is connected to the suction tube 202, the support plate 403 abuts against the bottom of the sampling bottle 101, and the connecting column rotates around its own axis, so that the connecting column pulls the distance between the support plate 403 and the main housing 205, so that the support plate 403 limits the combination consisting of the sampling bottle 101 and the detection tube 301 to be away from the suction tube 202, thereby ensuring the stability of the connection between the detection tube 301 and the suction tube 202.

Referring to Figures 3 to 5, in one embodiment of the present application, the diameter of the second outlet 303 is smaller than the inner diameter of the detection tube 301. In order to improve the portability of the filtration kit and reduce the weight of the staff, the power of the suction pump in the suction device 201 is limited, thereby keeping the volume of the suction pump at a certain level. However, this method causes the suction force generated by the suction pump to be constant. By making the diameter of the second outlet 303 smaller than the diameter of the detection tube 301, when the suction force that the suction pump can generate is constant, the smaller flow cross-sectional area of the second outlet 303 can ensure that a negative pressure can be generated at the second outlet 303, so that the negative pressure generated at the second outlet 303 can ensure that the water sample can pass through the filter membrane 304 relatively quickly, thereby ensuring the efficiency of filtration.

Furthermore, along the axial direction of the detection tube 301, the filter membrane 304 and the second outlet 303 are spaced apart, and the filter membrane 304 and the inner wall of the detection tube 301 form a filtration cavity 307, and the filtration cavity 307 is located between the filter membrane 304 and the second outlet 303, and is connected to the second outlet 303; the liquid entering the detection tube 301 through the first inlet 302, after being filtered by the filter membrane 304, leaves the detection tube 301 through the filtration cavity 307 and the second outlet 303 in turn.

Since the diameter of the second outlet 303 is smaller than the inner diameter of the detection tube 301, when the filter membrane 304 covers the internal channel of the detection tube 301, the projection of the second outlet 303 on the filter membrane 304 only covers a part of the area of the filter membrane 304. When there is a negative pressure at the second outlet 303, it can only produce a suction effect on a small part of the area of the filter membrane 304. By arranging the filter membrane 304 and the second outlet 303 at intervals, the filter membrane 304 and the inner wall of the detection tube 301 form a filtration cavity 307. When negative pressure is generated at the second outlet 303, negative pressure is generated in the filtration cavity 307, and the filtration cavity 307 covers a larger area of the filter membrane 304, thereby improving the utilization rate of the working area of the filter membrane 304. Of course, technicians in the technical field to which the present application belongs can understand that the volume of the filtration cavity 307 should not be too large so as not to affect the available volume of the detection tube 301, and at the same time, avoid affecting the suction effect in the filtration cavity 307.

Referring to Figures 3 to 5, in one embodiment of the present application, a support plate 308 is provided in the detection tube 301; the support plate 308 is provided between the filter membrane 304 and the second outlet 303, and the filter membrane 304 is provided on the end surface of the support plate 308 close to the first inlet 302; the support plate 308 is connected to the tube wall of the detection tube 301, and a support through hole 309 is provided on the end surface of the support plate 308; the liquid entering the detection tube 301 through the first inlet 302, after being filtered by the filter membrane 304, leaves the detection tube 301 through the support through hole 309 and the second outlet 303 in turn.

The filter membrane 304 can be a mixed cellulose filter membrane 304, or a polyethersulfone filter membrane 304 or other filter membranes 304. The support plate 308 is arranged between the filter membrane 304 and the second outlet 303. When there is suction at the second outlet 303, the support plate 308 can provide support for the filter membrane 304, reduce the degree of deformation of the filter membrane 304 due to the suction, thereby avoiding the filter membrane 304 from rupturing. At the same time, it avoids the situation where the water sample does not pass through the filtering effect of the filter membrane 304 due to the deformation of the filter membrane 304, but leaves the detection tube 301 from the gap between the filter membrane 304 and the wall of the detection tube 301.

Technicians in the technical field to which the present application belongs can understand that the support plate 308 can be spaced apart from the second outlet 303, the filter membrane 304 is arranged on the end face of the support plate 308 close to the first inlet 302, a filtration cavity 307 is formed between the support plate 308 and the second outlet 303, the support plate 308 and the inner wall of the detection tube 301 are spaced apart from the second outlet 303 via a support ring 310, see Figure 3, the filter membrane 304 is fixed to the end face of the support plate 308 by another support ring 310, and the support ring 310 can be connected to the inner wall of the detection tube 301 by threaded connection, clamping connection, or gluing.

Those skilled in the art of the present application can understand that the connection method in the present application is not limited to the direct connection method, but also includes the indirect connection method. For example, when the support frame 401 is detachably connected to the main body shell 205, the connecting column can be provided with a thread, and the main body shell 205 can be provided with a thread that matches the connecting column thread, so that the connecting column and the main body shell 205 are directly threadedly connected, or a frame structure connected to the main body shell 205 is provided in the main body shell 205, and the frame structure is provided with a thread that matches the connecting column thread, so that the connecting column is connected to the main body shell 205 through the frame structure. The structure is detachably connected to the main body housing 205; for another example, the second outlet 303 is plugged into the suction tube 202, so that the tube wall at the second outlet 303 end of the detection tube 301 is directly detachably connected to the suction tube 202, or the connecting straight tube 305 is plugged into the suction tube 202, and the connecting straight tube 305 is connected to the end face of the second outlet 303 end of the detection tube 301, the connecting straight tube 305 is connected to the second outlet 303, and the tube wall at the second outlet 303 end is indirectly detachably connected to the suction tube 202 through the connecting straight tube 305, which will not be repeated here.

Technicians in the technical field to which the present application belongs can understand that the detection tube 301 in the present application can be a detection tube made of PMMA material, the tube wall of the detection tube 301 is transparent, and light can be transmitted in the tube wall of the detection tube 301 and the liquid contained in the detection tube 301, so that the concentration of microorganisms in the detection tube can be detected using an optical detection element, and the detection tube can withstand higher temperatures, so that the liquid in the detection tube 301 can be heated to ensure that the temperature of the liquid in the detection tube is suitable for the cultivation of microorganisms.

The present application also relates to a method for using a portable suction filtration kit for water quality microorganism detection. The portable suction filtration kit for water quality conventional microorganism detection comprises a sampling container provided with a sampling port; and a detection tube provided with a first inlet at one end and a second outlet at the other end, the detection tube being provided with a filter membrane between the first inlet and the second outlet, and a suction device provided with a suction tube, the suction tube being arranged vertically;

Usage methods include:

S1: Collect water samples using sampling containers;

S2: The sampling port of the sampling container is set upward;

S3: providing a force so that the first inlet of the detection tube is disposed downward;

S4: The first inlet arranged downward is detachably connected to the sampling port arranged upward;

S5: providing a force to rotate the assembly consisting of the sampling container and the detection tube so that the second outlet of the detection tube faces downward;

S6: The second outlet facing downward is detachably connected to the suction tube, and the suction device sucks the combination of the sampling container and the detection tube through the suction tube, and the microorganisms in the water sample are retained on the filter membrane of the detection tube.

Technicians in the technical field to which the present application belongs can understand that the sampling container in the present application can be a sampling bottle or a sampling bag, and there are many ways to implement the detachable connection between the first inlet and the sampling port, and the detachable connection between the second outlet and the suction tube, such as the way in which the two can be plugged together through size matching, or the two can be provided with mutually matching threads and the two can be threadedly connected. The ways in which the first inlet and the sampling port can be detachably connected, and the second outlet and the suction tube can be detachably connected are not described here.

It should be understood that although this specification is described according to various implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

The series of detailed descriptions listed above are merely specific descriptions of feasible embodiments of the present application. They are not intended to limit the scope of protection of the present application. Any equivalent implementation scheme or changes that do not deviate from the technical spirit of the present application, such as combination, division or repetition of features, should be included in the scope of protection of the present application.

Claims (10)

1. The utility model provides a quality of water microorganism detects with portable suction filtration external member which characterized in that includes:

The sampling container is provided with a sampling port communicated with the internal cavity, and the sampling port is provided with a sampling connecting part;

A suction device comprising a suction tube having a suction effect, the suction tube being vertically disposed;

the device comprises a detection tube, a filter membrane and a filter membrane, wherein one end face of the detection tube is provided with a first inlet, the other end face of the detection tube is provided with a second outlet, and the filter membrane is arranged in the detection tube between the first inlet and the second outlet;

The pipe wall of the first inlet end of the detection pipe is detachably connected and matched with the sampling connecting part, and the pipe wall of the second outlet end of the detection pipe is detachably connected and matched with the suction pipe;

after the sampling container collects water samples, the first inlet is connected with the sampling port, the sampling container is vertically arranged with a combination body formed by the detection tube, the second outlet is connected with the suction tube, the water samples in the sampling container enter the suction device through the detection tube under the action of gravity and suction of the suction device, and microorganisms in the water samples are left in the detection tube under the action of filtration of the filter membrane.

2. The portable suction filtration kit for water quality microorganism detection according to claim 1, wherein,

The second outlet diameter is smaller than the inner diameter of the detection tube.

3. The portable suction filtration kit for water quality microorganism detection according to claim 2, wherein,

The filter membrane and the second outlet are arranged at intervals along the axial direction of the detection tube, a suction filtration cavity is formed by the filter membrane and the inner wall of the detection tube, and the suction filtration cavity is positioned between the filter membrane and the second outlet and is communicated with the second outlet;

And the liquid entering the detection tube through the first inlet sequentially passes through the suction filtration cavity and the second outlet to leave the detection tube after the liquid is filtered by the filter membrane.

4. The portable suction filtration kit for water quality microorganism detection according to claim 1, wherein,

A supporting plate is arranged in the detection tube;

The support plate is arranged between the filter membrane and the second outlet, and the filter membrane is arranged on the end face, close to the first inlet, of the support plate;

The support plate is connected with the pipe wall of the detection pipe, and the end face of the support plate is provided with a support through hole;

And the liquid entering the detection tube through the first inlet passes through the support through hole and the second outlet in sequence after the liquid is filtered by the filter membrane and leaves the detection tube.

5. The portable suction filtration kit for water quality microorganism detection according to claim 1, wherein,

The pipe wall of the first inlet end of the detection pipe is provided with a first thread;

The sampling connecting portion is provided with a second thread, and the second thread is in threaded connection and matched with the first thread.

6. The portable suction filtration kit for water quality microorganism detection according to claim 1, wherein,

The end face of the second outlet end of the detection tube is connected with a communication straight tube, the axial direction of the communication straight tube is the same as the axial direction of the detection tube, and the communication straight tube is communicated with the second outlet;

The communication straight pipe is in plug-in connection with the suction pipe, and the suction pipe can be communicated with the detection pipe through the communication straight pipe.

7. The portable suction filtration kit for water quality microorganism detection according to claim 6, wherein,

The end face of the second outlet end of the detection tube is also connected with a fixed tube, the inner diameter of the fixed tube is larger than the outer diameter of the communication straight tube, the communication straight tube is arranged in the fixed tube, and the second outlet, the communication straight tube and the fixed tube are coaxially arranged;

The outer diameter of the suction pipe is not larger than the inner diameter of the fixed pipe, the inner diameter of the suction pipe is not smaller than the outer diameter of the communication straight pipe, and the communication straight pipe can be inserted into the suction pipe to be in plug-in fit with the suction pipe.

8. The portable suction filtration kit for water quality microorganism detection according to claim 1, wherein,

The suction device comprises a mounting tube, wherein the mounting tube forms a vertically arranged accommodating hole, and the diameter of the accommodating hole is not smaller than that of the detection tube;

the suction pipe is arranged in the accommodating hole, and the accommodating hole and the suction pipe are coaxially arranged;

the detection tube can be inserted into the accommodating hole, and the inner wall of the accommodating hole limits the movement of the detection tube along the radial direction of the detection tube.

9. The portable suction filtration kit for water quality microorganism detection according to claim 1, wherein,

The filtering device also comprises a host shell and a supporting frame which is detachably connected with the host shell;

the sampling container is vertically arranged with the combination body formed by connecting the detection tube, and the support frame limits the movement of the sampling container and the detection tube when the sampling container is connected with the suction tube through the second outlet.

10. The portable suction filtration kit for detecting the water quality microorganisms comprises a sampling container provided with a sampling port; and

A detection tube with a first inlet at one end and a second outlet at the other end, a filter membrane arranged between the first inlet and the second outlet, and

The suction device is provided with a suction pipe which is vertically arranged;

The using method comprises the following steps:

s1: collecting a water sample by using a sampling container;

S2: the sampling port of the sampling container is upward;

S3: providing an acting force to enable the first inlet of the detection tube to be arranged downwards;

s4: the first inlet arranged downwards is detachably connected with the sampling port arranged upwards;

S5: providing an acting force to enable the assembly formed by the sampling container and the detection tube to rotate, so that the second outlet of the detection tube faces downwards;

s6: the downward second outlet is detachably connected with the suction pipe, the suction device sucks the combination body consisting of the sampling container and the detection pipe through the suction pipe, and microorganisms in the water sample are reserved at the filter membrane of the detection pipe.