CN118020937B - Beverage capable of clearing and nourishing throat and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of food, in particular to a beverage for clearing and nourishing throat and a preparation method thereof, the beverage consists of main materials of phyllanthus emblica extract, platycodon grandiflorum extract, honeysuckle extract, chrysanthemum extract, olive extract, auxiliary materials and purified water. The main ingredients of the beverage provided by the invention are prepared into the extract, the method can promote the absorption rate of raw material components in vivo, is beneficial to the absorption of active ingredients to human bodies so as to accelerate the generation of corresponding pharmacological actions, has obvious improvement effects on pharyngalgia, itching throat, dry throat, foreign body sensation and symptoms, has good taste of raw material components, does not have bitter taste raw material components, and the prepared beverage is clear and transparent, has no sediment, is light amber and has bright color.

Description

A throat-clearing and throat-moistening beverage and a preparation method thereof

Technical Field

The invention belongs to the technical field of food, and particularly relates to a throat-clearing and throat-moistening beverage and a preparation method thereof.

Background Art

In recent years, the fast-paced modern lifestyle has caused more and more throat problems, and pharyngitis has become a common chronic disease that troubles people's daily lives. The throat is a common passage for the respiratory tract and the digestive tract. Various external physical, chemical and biological factors can easily damage the throat mucosa, leading to inflammation of the throat mucosa. Currently, the drugs for treating throat discomfort symptoms have more or less side effects on the human body. In nature, there are many natural ingredients including plants and microorganisms that have the effect of clearing the throat and moisturizing the throat. Using natural plants in nature as raw materials and processing them into health foods with the function of clearing the throat and moisturizing the throat can not only treat the symptoms of throat discomfort, but also will not cause side effects to the human body.

At present, there are many kinds of throat-clearing and throat-soothing teas on the market. CN201210548597.4 discloses a throat-clearing and throat-soothing tea and its preparation method, which is composed of monk fruit extract, buddleja extract, oroxylum extract, dandelion extract, loblolly leaf extract, green fruit extract, and almond extract. However, buddleja extract, oroxylum and dandelion in the ingredients are bitter, and the taste is not suitable for long-term drinking.

Phyllanthus emblica is the dried mature fruit of Euphorbiaceae plants. It is mainly distributed in Fujian, Guangdong, Yunnan and other places. It is a traditional folk medicine. It has a history of 1,800 years of medicinal use in my country and is included in the Chinese Pharmacopoeia. It is mainly used to treat indigestion, abdominal pain, chronic hepatitis, gastroenteritis, hyperlipidemia and other diseases. In Southeast Asia, such as India and Sri Lanka, Phyllanthus emblica is widely used and has a high status in the medical system. Due to its good medicinal value and extremely high nutritional value, the Ministry of Health of China has included Phyllanthus emblica in the first list of medicines and foods. In recent years, the pharmacological research of Phyllanthus emblica has focused on antibacterial, antioxidant, anti-tumor and other fields.

Platycodon grandiflorum is the dried root of Platycodon grandiflorum of Campanulaceae. It was first recorded in Shennong's Herbal Classic and was listed as a low-grade product. It is included in all editions of the Chinese Pharmacopoeia and is one of the 40 commonly used bulk medicinal materials in my country. It is an item that is "both food and medicine" announced by the Ministry of Health. Pharmacological studies have shown that Platycodon grandiflorum has a significant protective effect on animals with liver damage induced by alcohol, carbon tetrachloride and high-fat diet.

Green fruit, also known as "olive", is named because the fruit can be eaten fresh when it is still green. It is mild in nature, sweet, astringent and sour in taste. It clears away heat, relieves sore throat, promotes the production of body fluids and detoxifies. It is used for sore throat, cough, thirst, fish and crab poisoning, etc.

Therefore, it is necessary to use plants in nature to develop a beverage that is suitable for long-term drinking, has a good taste, and has obvious throat clearing and soothing effects.

Summary of the invention

The present invention aims to provide a throat-clearing and throat-moistening beverage and a preparation method thereof in view of the problems of the prior art.

The specific technical solutions of the present invention are as follows:

A throat-clearing and throat-moistening beverage, comprising main ingredients of emblica extract, platycodon extract, honeysuckle extract, chrysanthemum extract, green fruit extract, auxiliary materials and purified water.

Preferably, the main ingredients comprise, by weight percentage, 50-55% of Phyllanthus emblica extract, 20-25% of Psoralea corylifolia extract, 10-15% of Honeysuckle extract, 5-10% of Chrysanthemum extract, and 2-5% of Platycodon grandiflorum extract.

Preferably, the emblica extract is prepared by the following steps: adding 1%-3% NaCl aqueous solution to emblica pulp, squeezing, concentrating the squeezed juice, and drying to obtain the emblica extract.

Preferably, the preparation method of the green fruit extract is as follows:

(1) taking green fruit, removing the core and crushing it, adding 60% to 70% ethanol aqueous solution by mass, with a liquid-to-solid ratio of 20:1 to 60:1, and hydrolyzing it at 20 to 50° C. for 3 to 6 hours to obtain an initial extraction solution;

(2) adding hydrochloric acid to the initial extract, and hydrolyzing at 50-90° C. for 5-9 hours to obtain a second extract having a pH value of 2-3;

(3) adding alkaline solution to the second extract to neutralize the solution until the pH is 5 to 6, and concentrating the solution;

(4) Extract the obtained concentrated solution with ethyl acetate, remove the aqueous phase, and concentrate the ethyl acetate phase to dryness to obtain a light yellow powder, which is the green fruit extract.

Preferably, the chrysanthemum extract is prepared by the following steps: taking chrysanthemum, refluxing and extracting it twice with a mass fraction of 50-70% ethanol aqueous solution, the reflux time is 1.5 and 1 hour respectively, combining the extracts, concentrating under reduced pressure, filtering with water, loading the filtrate into a macroporous adsorption resin column, gradient eluting with 3BV of water, 10wt% ethanol aqueous solution, and 70wt% ethanol aqueous solution respectively, collecting the eluate, concentrating under reduced pressure, and drying.

Preferably, the auxiliary materials are honey, vitamin C and nisin.

Preferably, the emblica extract and the green fruit extract are further processed into a mixture of emblica and green fruit vinegar, the steps of which are: taking the emblica extract and the green fruit extract in proportion, adding 3-5% white sugar based on the total mass of the emblica extract and the green fruit extract to prepare a slurry, inoculating active dry yeast for brewing, fermenting at room temperature until the alcohol content is 7%-11% vol, filtering the filtrate, inoculating acetic acid bacteria, and aerobically fermenting for 10-15 days to obtain the mixture of emblica and green fruit vinegar.

A method for preparing a throat-clearing and throat-moistening beverage comprises the following steps:

(1) Taking Phyllanthus emblica extract, Platycodon grandiflorum extract, Honeysuckle extract, Chrysanthemum extract and Green fruit extract in proportion and mixing them as the main material;

(2) Add beverage auxiliary materials and purified water to the main ingredients, degas and sterilize in an ultra-high temperature instantaneous sterilizer at a temperature of 121-131°C for 3-8 seconds, cool, add _CO2 or _not , and then aseptically fill to obtain the finished product.

Preferably, a method for preparing a throat-clearing and throat-moistening beverage comprises the following steps:

(1) taking an extract of emblica emblica and an extract of green fruit according to a certain proportion, adding 3-5% white sugar based on the total weight of the extract of emblica emblica and the green fruit extract to prepare a slurry, inoculating active dry yeast for brewing, fermenting at room temperature until the alcohol content is 7%-11% vol, filtering the filtrate, inoculating acetic acid bacteria, and aerobically fermenting for 10-15 days to obtain a mixture of emblica emblica and green fruit vinegar;

(2) taking honeysuckle extract, chrysanthemum extract, and platycodon extract and adding them into the mixture of step (1) to prepare a beverage main ingredient;

(3) Add beverage auxiliary materials and purified water to the main beverage ingredients, degas, sterilize in an ultra-high temperature instantaneous sterilizer at a temperature of 121-131°C for 3-8 seconds, cool, add _CO2 or _not , and then aseptically fill to obtain the finished product.

Preferably, the mass ratio of the beverage main ingredient, beverage auxiliary ingredient and purified water is 5-10:20-25:100.

The beneficial effects of the present invention are:

1) The main ingredients of the beverage provided by the present invention are all made into ultrafine powder extracts. This method can promote the absorption rate of the raw material ingredients in the body, which is beneficial to the absorption of the active ingredients by the human body and accelerates the production of corresponding effects.

2) The various ingredients of the present invention have health care effects on the throat, and the raw material ingredients have good taste, no bitter taste raw material ingredients, and the preparation method of each raw material extract of the present invention can effectively retain the beneficial substances of the raw materials. The present invention further processes the emblica extract and the green fruit extract into an emblica and green fruit vinegar mixture, and the prepared emblica and green fruit vinegar beverage is clear and transparent, has no precipitation, is light amber, has bright color, has rich fruit and vinegar aroma, has a soft sour taste, and has no astringency and other peculiar smells.

DETAILED DESCRIPTION

The present application is further described in detail below in conjunction with comparative examples and embodiments.

First, various beverage main ingredients are prepared, and the preparation method is as follows:

Preparation of the emblica extract: 4 kg of emblica pulp is added with 4000 ml of a 2% by mass NaCl aqueous solution for squeezing, the squeezed juice is concentrated, and the emblica extract is obtained by drying.

Preparation of green fruit extract:

(1) 3 kg of green fruit was taken, the core was removed and crushed, 70% ethanol aqueous solution was added, the liquid-to-solid ratio was 50:1, and the mixture was hydrolyzed at 35°C for 6 hours to obtain the initial extraction solution;

(2) adding hydrochloric acid to the initial extract and hydrolyzing at 70° C. for 7 hours to obtain a second extract having a pH value of 2;

(3) adding sodium hydroxide to the second extract to neutralize it until the pH reaches 6, and concentrating the solution;

(4) Extract the obtained concentrated solution with ethyl acetate, remove the aqueous phase, and concentrate the ethyl acetate phase to dryness to obtain a light yellow powder, which is the green fruit extract.

Preparation of chrysanthemum extract: Take 2 kg of chrysanthemum, reflux extract twice with a mass fraction of 60% ethanol aqueous solution, the reflux time is 1.5 and 1 hour respectively, the extracts are combined, concentrated under reduced pressure, filtered with water, the filtrate is loaded into a macroporous adsorption resin column, and gradient elution is performed with water, 10wt% ethanol aqueous solution, and 70wt% ethanol aqueous solution 3BV each, respectively, the eluate is collected, concentrated under reduced pressure, and dried.

Preparation of honeysuckle extract:

(1) Take honeysuckle medicinal material, add 18 times the amount of water to the medicinal material, perform steam distillation, collect 6 times the amount of distillate of the medicinal material, perform redistillation on the distillate, and collect 1.25 times the amount of redistillate of the medicinal material; filter the medicinal liquid in the distiller, reserve the filtrate, add 10 times the amount of water to the medicinal residue, boil for 1 hour, filter, and combine the filtrate;

(2) Take the combined filtrate, adjust the pH value to 6 with 10 wt% _{Na2CO3 aqueous} solution, apply it to a macroporous resin column, the amount of the resin used is twice the amount of the medicinal material, collect the effluent, adjust the pH value to 2.5 with hydrochloric acid, continue to apply it to a macroporous resin column, and then elute with injection water twice the amount of the resin, discard the water eluate, and then elute with 70 wt% ethanol on the column, collect the alcohol eluate, and recover it until there is no alcohol taste, thereby obtaining the honeysuckle extract.

Platycodon grandiflorum extract was purchased from Shanghai Xinkai Pharmaceutical Technology Co., Ltd.

The beverage prepared above is further prepared into a beverage for clearing the throat and moistening the throat.

Example 1

Preparation of a throat-clearing and throat-moistening beverage: take the beverage main ingredients according to the following weight percentages: 50% of Phyllanthus emblica extract, 25% of Platycodon grandiflorum extract, 10% of Honeysuckle extract, 10% of Chrysanthemum extract, and 5% of Green Fruit extract. Then, add the beverage main ingredients, beverage auxiliary ingredients, and purified water in a mass ratio of 10:20:100, add the beverage auxiliary ingredients and purified water to the main ingredients, degas, and sterilize in an ultra-high temperature instantaneous sterilizer at a sterilization temperature of 125°C for 5 seconds, cool, add CO ₂ , and then perform aseptic filling to obtain the finished product.

Example 2

Preparation of a throat-clearing and throat-moistening beverage: take the beverage main ingredients according to the following weight percentages: 55% of Phyllanthus emblica extract, 20% of Platycodon grandiflorum extract, 15% of Honeysuckle extract, 5% of Chrysanthemum extract, and 5% of Green Fruit extract. Then, add the beverage main ingredients, beverage auxiliary ingredients, and purified water in a mass ratio of 10:20:100, add the beverage auxiliary ingredients and purified water to the main ingredients, degas, and sterilize in an ultra-high temperature instantaneous sterilizer at a sterilization temperature of 125°C for 5 seconds, cool, add CO ₂ , and then perform aseptic filling to obtain the finished product.

Example 3

Preparation of a throat-clearing and throat-moistening beverage: take the beverage main ingredients according to the following weight percentages: 53% of Phyllanthus emblica extract, 24% of Platycodon grandiflorum extract, 13% of Honeysuckle extract, 5% of Chrysanthemum extract, and 5% of Green Fruit extract. Then, add the beverage main ingredients, beverage auxiliary ingredients, and purified water in a mass ratio of 8:23:100, add the beverage auxiliary ingredients and purified water to the main ingredients, degas, and sterilize in an ultra-high temperature instantaneous sterilizer at a sterilization temperature of 130°C for 6 seconds, cool, add CO ₂ , and then perform aseptic filling to obtain the finished product.

Example 4

The difference from Example 1 is that 50% of the emblica extract and 25% of the platycodon extract in the main raw materials are further prepared into a mixture of emblica and green fruit vinegar, 5% of the total mass of the emblica extract and the platycodon extract is added with white sugar for slurry preparation, 3% of the total mass of the emblica extract and the platycodon extract is introduced into active dry yeast for brewing, fermented at room temperature until the alcohol content is 10% vol, the filtrate is filtered to obtain the filtrate, 0.5% of the total mass of the filtrate is introduced into acetic acid bacteria, and aerobically fermented for 15 days to obtain the emblica and green fruit vinegar mixture.

Example 5

The difference from Example 2 is that 55% of the emblica extract and 20% of the platycodon extract in the main raw materials are further prepared into a mixture of emblica and green fruit vinegar, 4% of the total weight of the emblica extract and the platycodon extract is added with white sugar for slurry preparation, 3% of the total weight of the emblica extract and the platycodon extract is introduced into active dry yeast for brewing, fermented at room temperature to an alcohol content of 11% vol, the filtrate is filtered to obtain the filtrate, 0.5% of the total weight of the filtrate is introduced into acetic acid bacteria, and aerobically fermented for 14 days to obtain the emblica and green fruit vinegar mixture.

Example 6

The difference from Example 2 is that 53% of the emblica extract and 24% of the platycodon extract in the main raw materials are further prepared into a mixture of emblica and green fruit vinegar, 3% of the total mass of the emblica extract and the platycodon extract is added with white sugar for slurry preparation, 3% of the total mass of the emblica extract and the platycodon extract is inoculated with brewing active dry yeast, fermented at room temperature until the alcohol content is 10% vol, the filtrate is filtered, 0.5% of the total mass of the filtrate is inoculated with acetic acid bacteria, and aerobically fermented for 13 days to obtain the emblica and green fruit vinegar mixture.

Comparative Example 1

By weight, take 50% of Phyllanthus emblica, 25% of Platycodon grandiflorum, 10% of Honeysuckle, 10% of Chrysanthemum, and 5% of Green Fruit according to the following weight fractions, decoct for 3 times, each time for 2 hours, filter and combine the decoctions.

Comparative Example 2

By weight, take 55% of Phyllanthus emblica, 20% of Platycodon grandiflorum, 15% of Honeysuckle, 5% of Chrysanthemum, and 5% of Green Fruit according to the following weight fractions, decoct for 3 times, each time for 2 hours, filter and combine the decoctions.

The application effect of the present invention is described in detail below in combination with sensory evaluation and related experiments.

A tasting panel consisting of 10 food professionals with training and experience in sensory evaluation was selected to conduct sensory evaluation on the beverages of Examples 1-6 and the decoctions of Comparative Examples 1-2. The total scores of color (20%), aroma (20%), tissue morphology (20%), and taste (40%) were calculated, which was the sensory evaluation index score (average score). The scoring criteria are shown in the following table. Table 1 shows the sensory evaluation criteria and evaluation results.

Table 1 Sensory evaluation standards and evaluation results

Through sensory evaluation, the raw material components selected by the present invention have good taste and no bitter taste raw material components. By further processing the raw materials into extracts, the color, aroma and texture are improved. The emblica extract and the green fruit extract are further processed into an emblica and green fruit vinegar mixture, which further improves the color, taste, aroma and texture, and is a beverage in the best state.

Throat clearing and moisturizing effect test

The main instruments and reagents used in the experiment are: xylene, analytical grade, Xi'an Chemical Reagent Factory; dexamethasone, for medical use, Tianjin Tianyao Pharmaceutical Co., Ltd.

Effect Example 1 Mouse ear swelling experiment

80 mice of both sexes were used, and adaptive feeding was performed for 5 days. After feeding, they were randomly divided into 10 groups, each with 8 mice, which were respectively set as a blank group, a positive control group, an experimental group of Examples 1-6, and an experimental group of Comparative Examples 1-2.

Experimental method: After applying xylene to the auricle of mice every day, each group of experimental mice was gavaged at a dose of 0.02 mL/g every day. For 30 days of the experiment, the positive control group took dexamethasone, wherein the dexamethasone concentration was 0.05%, the blank group took normal saline, and the rest took the throat clearing beverages of Examples 1-6 and Comparative Examples 1-2, respectively. The weight of the ear pieces of each mouse in each group was measured, and the average value of each group was taken.

Auricle swelling inhibition rate/%=(blank group ear piece weight-experimental group ear piece weight)/blank group ear piece weight×100%. The results are shown in Table 2.

Table 2 Effect of Qingyan beverage on the inhibition rate of ear swelling in mice

As shown in Table 2, the auricle swelling inhibition rate of Examples 1-6 is significant, and the effect of the beverage composed of the raw material extract is much higher than that of the decoction.

Effect Example 2: Throat Clearing Effect in Patients with Pharyngitis

Typical symptoms of pharyngitis (example): Zhang, male, 35 years old, smoked for 10 years, came to the hospital for intermittent dry throat and pharyngitis with sore throat for 1 year, usually healthy, symptoms: intermittent dry throat and itchy throat. Xia, female, 48 years old, worker, came to the hospital for "dry throat, itchy throat and foreign body sensation in the throat for 3 years". The patient had intermittent dry throat, itchy throat and foreign body sensation in the throat, could not spit out or swallow, often recurred, symptoms were obvious when irritable, and eating was normal. Diagnosis: chronic pharyngitis.

The beverages prepared in Example 1 and Example 4 were further tested for their throat clearing and throat moistening effects, and the method was as follows: subjects with typical symptoms of pharyngitis were selected and randomly divided into 3 groups according to the symptoms, with 30 cases in each group. The control group took Jinsuozi lozenges according to the instructions, 1 tablet each time, 2 times a day; the experimental group (2 groups took the beverages prepared in Example 1 and Example 4, respectively) took the throat clearing and throat moistening beverage of the present invention, one bottle of 200 ml per day. Efficacy judgment standard: refer to the "Guidelines for Clinical Research of New Chinese Medicines" issued by the Ministry of Health.

Markedly effective: symptoms are alleviated or disappeared, pharyngeal signs are alleviated by 2 levels, and the single symptom score is reduced by ≥4 points;

Effective: Symptoms are alleviated, pharyngeal signs are alleviated by 1-2 levels, and the single symptom score is reduced by 2-3 points;

Ineffective: No changes in symptoms and signs, and the score of a single symptom item decreases by <2 points.

The overall effect evaluation and symptom improvement results are shown in Table 3 below.

Table 3. Overall effect evaluation results

The results show that the throat-clearing and soothing beverage provided by the present invention can improve throat discomfort, and has a significant effect on symptoms such as throat pain, throat itching, dry throat, and foreign body sensation, especially the improvement effect on throat itching and foreign body sensation is more significant, with an effective rate of over 83%.

Claims (4)

1. A throat-clearing and throat-moistening beverage, characterized in that the beverage is composed of main ingredients of emblica extract, platycodon extract, honeysuckle extract, chrysanthemum extract, green fruit extract, auxiliary materials and purified water; The main ingredients include 50-55% of Phyllanthus emblica extract, 20-25% of Psoralea corylifolia extract, 10-15% of Honeysuckle extract, 5-10% of Chrysanthemum extract, and 2-5% of Platycodon grandiflorum extract by weight percentage; The emblica extract is prepared by the following steps: adding 1%-3% NaCl aqueous solution to emblica pulp, squeezing, concentrating the squeezed juice, and drying to obtain the emblica extract; The preparation method of the green fruit extract is as follows: (1) Take green fruit, remove the core and crush it, add 60% to 70% ethanol aqueous solution, the liquid-to-solid ratio is 20:1 to 60:1, hydrolyze at 20 to 50°C for 3 to 6 hours, and obtain the initial extraction solution; (2) adding hydrochloric acid to the initial extract, and hydrolyzing at 50-90° C. for 5-9 hours to obtain a second extract having a pH value of 2-3; (3) adding alkaline solution to the second extract to neutralize the solution until the pH is 5 to 6, and concentrating the solution; (4) extracting the obtained concentrated solution with ethyl acetate, removing the aqueous phase, concentrating the ethyl acetate phase and drying it to obtain a light yellow powder, which is the green fruit extract; The chrysanthemum extract is prepared by the following steps: taking chrysanthemum, refluxing and extracting it twice with a mass fraction of 50-70% ethanol aqueous solution, the reflux time is 1.5 and 1 hour respectively, combining the extracts, concentrating under reduced pressure, filtering with water, loading the filtrate into a macroporous adsorption resin column, gradient eluting with water, 10wt% ethanol aqueous solution, and 70wt% ethanol aqueous solution, 3BV each, respectively, collecting the eluate, concentrating under reduced pressure, and drying to obtain the extract; The emblica extract and green fruit extract are further processed into a mixture of emblica and green fruit vinegar, and the steps are: take the emblica extract and green fruit extract in proportion, add 3-5% white sugar based on the total weight of the emblica extract and the green fruit extract to prepare a slurry, add active dry yeast for brewing, ferment at room temperature until the alcohol content is 7%-11% vol, filter the filtrate, add acetic acid bacteria, and aerobically ferment for 10-15 days to obtain the mixture of emblica and green fruit vinegar. 2. The throat-clearing and throat-moistening beverage according to claim 1, characterized in that the auxiliary materials are honey, vitamin C, and nisin. 3. A method for preparing the throat-clearing and throat-moistening beverage according to claim 1 or 2, characterized in that it comprises the following steps: (1) taking an extract of emblica buds and an extract of green fruit according to a certain proportion, adding 3-5% of the total weight of white sugar to prepare a slurry, adding active dry yeast for brewing, fermenting at room temperature until the alcohol content is 7%-11%vol, filtering the filtrate, adding acetic acid bacteria, and aerobically fermenting for 10-15 days to obtain a mixture of emblica buds and green fruit vinegar; (2) taking honeysuckle extract, chrysanthemum extract, and platycodon extract and adding them into the mixture of step (1) to prepare a beverage main ingredient; (3) Add beverage auxiliary materials and purified water to the main beverage ingredients, degas, and sterilize in an ultra-high temperature instantaneous sterilizer at a temperature of 121-131°C for 3-8 seconds. Cool, add _CO2 or _not , and then aseptically fill to obtain the finished product. 4. The preparation method according to claim 3 is characterized in that the mass ratio of the beverage main ingredient, beverage auxiliary material and purified water added is 5-10:20-25:100.