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CN118020762A - Stem cell storage protection extraction element - Google Patents

Stem cell storage protection extraction element Download PDF

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Publication number
CN118020762A
CN118020762A CN202410429528.4A CN202410429528A CN118020762A CN 118020762 A CN118020762 A CN 118020762A CN 202410429528 A CN202410429528 A CN 202410429528A CN 118020762 A CN118020762 A CN 118020762A
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shaped
special
stem cell
annular
disc
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CN118020762B (en
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孙继磊
黄妍
蔡天米
曹艺凡
周慧
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Yunnan Cell Innovation Biotechnology Co ltd
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Yunnan Cell Innovation Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • A01N1/14Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/142Apparatus
    • A01N1/144Apparatus for temperature control, e.g. refrigerators or freeze-drying apparatus

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
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Abstract

The invention relates to the technical field of stem cell storage, and discloses a stem cell storage protection extraction device, which comprises a special-shaped discharging structure and a pressure control type sealing structure, wherein a disc-shaped cover capable of being placed in a cover groove, and an inner elastic air film and an outer elastic air film for performing conflict type sealing on a relative movement gap are arranged in the special-shaped discharging structure; and the maximum air pressure control structure is internally provided with a valve plate which can block air in the pressure control type sealing structure and realize upward flow of the air when the air moves upwards and a spiral spring which generates damping effect on upward movement of the valve plate. This protection extraction element is stored to stem cell can enough guarantee when guaranteeing gas tightness in the storage process, can guarantee the sealed effect to nitrogen gas in getting the stem cell test tube of putting again, and then reduces when storing and getting the stem cell test tube and put, the leakage volume of nitrogen gas to reduce the temperature influence to nitrogen gas.

Description

一种干细胞储存保护提取装置A stem cell storage, protection and extraction device

技术领域Technical Field

本发明涉及干细胞储存技术领域,具体为一种干细胞储存保护提取装置。The present invention relates to the technical field of stem cell storage, and in particular to a stem cell storage, protection and extraction device.

背景技术Background technique

干细胞是一类具有自我复制能力的多潜能细胞,在一定条件下,它可以分化成多种功能细胞,因此,对于刚出生的婴儿,会保留一定量的脐带血以便提取其中的造血干细胞进行冻存;但现有储存干细胞的容器防护性能不足,而且在取用干细胞试管时,容器内的液氮会大量流失,导致容器内的温度升高,影响干细胞的储存。Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. Therefore, for newborn babies, a certain amount of umbilical cord blood will be retained in order to extract hematopoietic stem cells for freezing. However, the existing containers for storing stem cells have insufficient protective performance, and when taking out the stem cell test tubes, the liquid nitrogen in the container will be lost in large quantities, causing the temperature inside the container to rise, affecting the storage of stem cells.

为此,公开号为“CN208030123U”的中国专利公布了“一种干细胞储存保护提取装置”,其主要结构包括储存箱以及安装于储存箱内的干细胞提取机构,所述储存箱内安装有防护框架,防护框架内安装有干细胞提取机构,干细胞提取机构由提取筒、把手、固定柱和存放板组成,提取筒的一端插装于防护框架内,把手安装于提取筒的上端,固定柱安装于提取筒内,存放板包括上层板和下层板,上层板与下层板的中部开孔,开孔直径与固定柱的外径相匹配,上层板与下层板依次套装于固定柱上并通过螺丝螺孔固定。上述干细胞储存保护提取装置,整个过程,操作简单、使用方便,避免了在取用干细胞试管时,容器内的液氮会大量流失,导致容器内的温度升高,影响干细胞储存的问。To this end, the Chinese patent with the publication number "CN208030123U" announced "a stem cell storage protection extraction device", whose main structure includes a storage box and a stem cell extraction mechanism installed in the storage box, a protective frame is installed in the storage box, and a stem cell extraction mechanism is installed in the protective frame. The stem cell extraction mechanism consists of an extraction tube, a handle, a fixed column and a storage plate. One end of the extraction tube is inserted into the protective frame, the handle is installed at the upper end of the extraction tube, and the fixed column is installed in the extraction tube. The storage plate includes an upper plate and a lower plate. The middle part of the upper plate and the lower plate has a hole, and the hole diameter matches the outer diameter of the fixed column. The upper plate and the lower plate are sequentially mounted on the fixed column and fixed by screw holes. The above-mentioned stem cell storage protection extraction device has a simple operation and convenient use throughout the process, which avoids the problem that when taking out the stem cell test tube, the liquid nitrogen in the container will be lost in large quantities, causing the temperature in the container to rise, affecting the storage of stem cells.

上述干细胞储存保护提取装置在实际使用时,当需提取干细胞试管时,通过拧转把手使销轴脱离凹槽后,缓慢将提取筒拉出,当听到销轴再一次顶压在凹槽上产生的响声时,停止拉出动作,此时提取筒固定于储存箱外,操作人员便可取出提取筒内放置的干细胞试管,但是,在当干细胞提取机构在瓶口运动时,氮气仍然会沿机构缝隙处向上泄露,导致其对于氮气的封闭性能下降。When the above-mentioned stem cell storage protection and extraction device is actually used, when it is necessary to extract a stem cell test tube, the handle is turned to disengage the pin from the groove, and the extraction tube is slowly pulled out. When a sound is heard when the pin presses against the groove again, the pulling action is stopped. At this time, the extraction tube is fixed outside the storage box, and the operator can take out the stem cell test tube placed in the extraction tube. However, when the stem cell extraction mechanism moves at the bottle mouth, nitrogen will still leak upward along the gap of the mechanism, resulting in a decrease in its sealing performance for nitrogen.

发明内容Summary of the invention

针对现有技术的不足,本发明提供了一种干细胞储存保护提取装置,既能够保证在储存过程中保证气体密封性的同时,又能够在取放干细胞试管保证对氮气的封闭效果,进而降低在对干细胞试管储存和取放时,氮气的泄露量,从而降低对氮气的温度影响,解决了上述技术问题。In view of the deficiencies in the prior art, the present invention provides a stem cell storage, protection and extraction device, which can not only ensure gas sealing during the storage process, but also ensure the sealing effect of nitrogen when taking out and placing stem cell test tubes, thereby reducing the leakage of nitrogen when storing and taking out and placing stem cell test tubes, thereby reducing the temperature impact of nitrogen, and solving the above-mentioned technical problems.

为实现上述目的,本发明提供如下技术方案:一种干细胞储存保护提取装置,包括用于储存液氮的液氮储存罐、设置于液氮储存罐内部的液氮储存腔、设置于液氮储存罐顶端中心的异形取放口、设置于异形取放口顶端的盖槽以及设置于异形取放口底部外围的环形嵌入槽,还包括异形放料结构,其内部设置有用于放置干细胞试管的试管放置槽以及用于限定试管放置槽固定在取放位置的底部环形永磁体和顶部环形永磁体;控压式密封结构,其内部设置有能够安放于盖槽内部的盘形盖以及用于对相对运动间隙进行抵触式封闭的内弹性气膜和外弹性气膜;以及最大气压控制结构,其内部设置有能够对位于控压式密封结构内部的气体进行堵塞且在向上移动时实现气体向上流动的阀板以及对阀板向上的运动产生阻尼效应的螺旋弹簧。To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a stem cell storage, protection and extraction device, comprising a liquid nitrogen storage tank for storing liquid nitrogen, a liquid nitrogen storage cavity arranged inside the liquid nitrogen storage tank, a special-shaped access opening arranged at the center of the top of the liquid nitrogen storage tank, a cover groove arranged at the top of the special-shaped access opening, and an annular embedding groove arranged at the periphery of the bottom of the special-shaped access opening, and also comprising a special-shaped discharge structure, wherein a test tube placement groove for placing stem cell test tubes and a bottom annular permanent magnet and a top annular permanent magnet for limiting the test tube placement groove to be fixed at a access position are arranged inside; a pressure-controlled sealing structure, wherein a disc-shaped cover that can be placed inside the cover groove and an inner elastic gas film and an outer elastic gas film for contact-type sealing of a relative motion gap are arranged inside; and a maximum air pressure control structure, wherein a valve plate that can block the gas inside the pressure-controlled sealing structure and realize the upward flow of gas when moving upward and a coil spring that produces a damping effect on the upward movement of the valve plate are arranged inside.

优选的,所述异形放料结构包括由铜材料制成的异形柱和顶部环形永磁体,所述异形柱在靠近其圆周面的部位设置有多个环形阵列式且用于放置干细胞试管的试管放置槽,所述异形柱的底部中心设置有向上凹陷的空心槽,所述异形柱的上端面中心设置有限位连接柱,所述限位连接柱的顶端固定安装有活动杆,所述活动杆的顶端设置有与其一体式结构的限位活动板,所述异形柱的底部外围设置有一体式结构且向外围凸起的限位活动环,所述限位活动环的上端面和环形嵌入槽的内部分别嵌入有底部环形永磁体和顶部环形永磁体。Preferably, the special-shaped discharge structure includes a special-shaped column made of copper material and a top annular permanent magnet, the special-shaped column is provided with a plurality of test tube placement grooves in an annular array for placing stem cell test tubes at a position close to its circumferential surface, the bottom center of the special-shaped column is provided with a hollow groove recessed upward, the center of the upper end surface of the special-shaped column is provided with a limiting connecting column, a movable rod is fixedly installed on the top of the limiting connecting column, a limiting movable plate with an integral structure therewith is provided on the top of the movable rod, a limiting movable ring with an integral structure and protruding toward the periphery is provided on the periphery of the bottom of the special-shaped column, the upper end surface of the limiting movable ring and the inside of the annular embedding groove are respectively embedded with the bottom annular permanent magnet and the top annular permanent magnet.

优选的,所述异形柱圆周面的结构外形与所述异形取放口的结构外形一致、均为梅花状结构,且异形柱圆周面的结构尺寸与所述异形取放口的结构尺寸匹配。Preferably, the structural shape of the circumferential surface of the special-shaped column is consistent with the structural shape of the special-shaped access opening, both are plum blossom structures, and the structural dimensions of the circumferential surface of the special-shaped column match the structural dimensions of the special-shaped access opening.

优选的,所述异形柱的高度高于异形取放口的高度。Preferably, the height of the special-shaped column is higher than the height of the special-shaped loading and unloading opening.

优选的,所述限位连接柱的高度低于异形取放口的高度,且所述限位连接柱的高度高于干细胞试管安放在试管放置槽内部后、干细胞试管顶端的水平高度。Preferably, the height of the limiting connection column is lower than the height of the special-shaped access opening, and the height of the limiting connection column is higher than the horizontal height of the top of the stem cell test tube after the stem cell test tube is placed in the test tube placement groove.

优选的,所述底部环形永磁体上环形端面的磁性和顶部环形永磁体下环形端面的磁性相反。Preferably, the magnetic properties of the upper annular end surface of the bottom annular permanent magnet are opposite to the magnetic properties of the lower annular end surface of the top annular permanent magnet.

优选的,所述控压式密封结构包括能够安放于盖槽内部的盘形盖,所述盘形盖的中心设置有用于套放活动杆的杆孔,所述盘形盖的内部设置有环形气体流动腔,所述盘形盖在位于所述环形气体流动腔的内侧设置有连通杆孔的内环形气体储存腔,所述盘形盖在位于所述环形气体流动腔的外侧设置有连通盘形盖外圆周面的外环形气体储存腔,所述环形气体流动腔和内环形气体储存腔之间通过多个第一气体流动孔连通,所述环形气体流动腔和外环形气体储存腔之间通过多个第二气体流动孔连通,所述盘形盖在位于环形气体流动腔和杆孔的交汇处嵌入有内弹性气膜,所述盘形盖在位于所述外环形气体储存腔的圆周边缘部位嵌入有外弹性气膜,所述盘形盖中设置有连通环形气体流动腔且内部安装有气阀的气体注入孔,所述盘形盖的内部设置有连通其上端面和环形气体流动腔的通气口。Preferably, the pressure-controlled sealing structure includes a disc-shaped cover that can be placed inside the cover groove, the center of the disc-shaped cover is provided with a rod hole for housing a movable rod, the interior of the disc-shaped cover is provided with an annular gas flow chamber, the disc-shaped cover is provided with an inner annular gas storage chamber connected to the rod hole on the inner side of the annular gas flow chamber, the disc-shaped cover is provided with an outer annular gas storage chamber connected to the outer circumferential surface of the disc-shaped cover on the outer side of the annular gas flow chamber, the annular gas flow chamber and the inner annular gas storage chamber are connected by a plurality of first gas flow holes, the annular gas flow chamber and the outer annular gas storage chamber are connected by a plurality of second gas flow holes, the disc-shaped cover is embedded with an inner elastic gas membrane at the intersection of the annular gas flow chamber and the rod hole, the disc-shaped cover is embedded with an outer elastic gas membrane at the circumferential edge of the outer annular gas storage chamber, the disc-shaped cover is provided with a gas injection hole connected to the annular gas flow chamber and having an air valve installed therein, and the disc-shaped cover is provided with an air vent connecting its upper end surface and the annular gas flow chamber.

优选的,所述内弹性气膜和外弹性气膜均为具备弹性的橡胶材料制成,且所述内弹性气膜的弹性强度为所述外弹性气膜的弹性强度的二倍以上。Preferably, the inner elastic air membrane and the outer elastic air membrane are both made of elastic rubber material, and the elastic strength of the inner elastic air membrane is more than twice the elastic strength of the outer elastic air membrane.

优选的,所述最大气压控制结构包括纵向空心柱,所述纵向空心柱的底端设置有与其一体式结构且固定安装在盘形盖上端面的底部固定板,所述纵向空心柱的内部设置有部件活动空腔,所述纵向空心柱的底端设置有连通部件活动空腔和通气口的进气孔,所述纵向空心柱的顶端设置有连通部件活动空腔和其上端面的排气孔,所述纵向空心柱在位于所述部件活动空腔的内部安放有能够沿部件活动空腔纵向活动的阀板,所述阀板的边缘设置有气压流动槽,所述阀板的上方安放有处于压缩状态的螺旋弹簧。Preferably, the maximum air pressure control structure includes a longitudinal hollow column, the bottom end of the longitudinal hollow column is provided with a bottom fixing plate which is an integral structure with the longitudinal hollow column and fixedly mounted on the upper end surface of the disc-shaped cover, the interior of the longitudinal hollow column is provided with a component movable cavity, the bottom end of the longitudinal hollow column is provided with an air inlet hole connecting the component movable cavity and the air vent, the top end of the longitudinal hollow column is provided with an exhaust hole connecting the component movable cavity and its upper end surface, the longitudinal hollow column is provided with a valve plate which can move longitudinally along the component movable cavity, the edge of the valve plate is provided with an air pressure flow groove, and a coil spring in a compressed state is provided above the valve plate.

优选的,所述阀板的中心线与所述气压流动槽之间的间距大于所述进气孔的结构半径。Preferably, the distance between the center line of the valve plate and the air pressure flow groove is greater than the structural radius of the air inlet hole.

与现有技术相比,本发明提供了一种干细胞储存保护提取装置,具备以下有益效果:Compared with the prior art, the present invention provides a stem cell storage, protection and extraction device, which has the following beneficial effects:

该干细胞储存保护提取装置,既能够保证在储存过程中保证气体密封性的同时,又能够在取放干细胞试管保证对氮气的封闭效果,进而降低在对干细胞试管储存和取放时,氮气的泄露量,从而降低对氮气的温度影响。The stem cell storage protection and extraction device can not only ensure gas sealing during the storage process, but also ensure the sealing effect of nitrogen when taking out and placing the stem cell test tube, thereby reducing the leakage of nitrogen when storing and taking out the stem cell test tube, thereby reducing the temperature impact on the nitrogen.

附图说明BRIEF DESCRIPTION OF THE DRAWINGS

图1为本发明的立体图;Fig. 1 is a perspective view of the present invention;

图2为本发明的立体剖面图;Fig. 2 is a three-dimensional cross-sectional view of the present invention;

图3为本发明中异形放料结构的立体图;FIG3 is a perspective view of a special-shaped discharge structure in the present invention;

图4为本发明中异形放料结构的立体剖面图;FIG4 is a three-dimensional cross-sectional view of the special-shaped discharge structure of the present invention;

图5为本发明中控压式密封结构的立体图;FIG5 is a perspective view of a pressure-controlled sealing structure of the present invention;

图6为本发明中控压式密封结构的立体剖面图;FIG6 is a three-dimensional cross-sectional view of the pressure-controlled sealing structure of the present invention;

图7为本发明中最大气压控制结构的立体剖面图。FIG. 7 is a three-dimensional cross-sectional view of the maximum atmospheric pressure control structure of the present invention.

其中:1、液氮储存罐;2、液氮储存腔;3、异形取放口;4、盖槽;5、环形嵌入槽;6、异形放料结构;61、异形柱;62、试管放置槽;63、空心槽;64、限位连接柱;65、活动杆;66、限位活动板;67、限位活动环;68、底部环形永磁体;69、顶部环形永磁体;7、控压式密封结构;71、盘形盖;72、杆孔;73、环形气体流动腔;74、内环形气体储存腔;75、外环形气体储存腔;76、第一气体流动孔;77、第二气体流动孔;78、内弹性气膜;79、外弹性气膜;710、气体注入孔;711、通气口;8、最大气压控制结构;81、纵向空心柱;82、底部固定板;83、部件活动空腔;84、进气孔;85、排气孔;86、阀板;87、气压流动槽;88、螺旋弹簧。Among them: 1. Liquid nitrogen storage tank; 2. Liquid nitrogen storage chamber; 3. Special-shaped access port; 4. Cover groove; 5. Annular embedded groove; 6. Special-shaped discharge structure; 61. Special-shaped column; 62. Test tube placement groove; 63. Hollow groove; 64. Limit connecting column; 65. Movable rod; 66. Limit movable plate; 67. Limit movable ring; 68. Bottom annular permanent magnet; 69. Top annular permanent magnet; 7. Pressure-controlled sealing structure; 71. Disc cover; 72. Rod hole; 73. Annular gas flow cavity; 74, inner annular gas storage cavity; 75, outer annular gas storage cavity; 76, first gas flow hole; 77, second gas flow hole; 78, inner elastic air membrane; 79, outer elastic air membrane; 710, gas injection hole; 711, air vent; 8, maximum air pressure control structure; 81, longitudinal hollow column; 82, bottom fixing plate; 83, component movable cavity; 84, air inlet hole; 85, exhaust hole; 86, valve plate; 87, air pressure flow groove; 88, coil spring.

具体实施方式Detailed ways

下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

请参阅图1和图2,一种干细胞储存保护提取装置,包括用于储存液氮的液氮储存罐1、设置于液氮储存罐1内部的液氮储存腔2、设置于液氮储存罐1顶端中心的异形取放口3、设置于异形取放口3顶端的盖槽4以及设置于异形取放口3底部外围的环形嵌入槽5,将液氮倒入至液氮储存腔2的内部,且位于液氮储存腔2内部的量需要符合实际需求。Please refer to Figures 1 and 2, a stem cell storage, protection and extraction device includes a liquid nitrogen storage tank 1 for storing liquid nitrogen, a liquid nitrogen storage chamber 2 arranged inside the liquid nitrogen storage tank 1, a special-shaped access port 3 arranged at the top center of the liquid nitrogen storage tank 1, a cover groove 4 arranged at the top of the special-shaped access port 3 and an annular embedded groove 5 arranged at the bottom periphery of the special-shaped access port 3. Liquid nitrogen is poured into the liquid nitrogen storage chamber 2, and the amount located inside the liquid nitrogen storage chamber 2 needs to meet actual needs.

为了实现对干细胞试管的封闭式驱动效果,请参阅图1、图2、图3和图4,需要设置异形放料结构6,其内部设置有用于放置干细胞试管的试管放置槽62以及用于限定试管放置槽62固定在取放位置的底部环形永磁体68和顶部环形永磁体69,在取放干细胞试管时,需要向上提拉限位活动板66,此时,异形柱61向上移动,异形柱61的上端面会首先插入至异形取放口3的内部,由于所述异形柱61圆周面的结构外形与所述异形取放口3的结构外形一致、均为梅花状结构,且异形柱61圆周面的结构尺寸与所述异形取放口3的结构尺寸匹配,异形柱61的圆周面能够实现内、外空间的隔绝效果,从而防止氮气沿运动缝隙处泄露,继续向上运动,由于所述限位连接柱64的高度低于异形取放口3的高度,且所述限位连接柱64的高度高于干细胞试管安放在试管放置槽62内部后、干细胞试管顶端的水平高度,会使得限位连接柱64首先抵触在盘形盖71的底部,直至盘形盖71被顶开,此时,异形柱61能够接替盘形盖71的封闭功能,从而从而防止氮气沿运动缝隙处泄露,降低对氮气的温度影响,由于所述底部环形永磁体68上环形端面的磁性和顶部环形永磁体69下环形端面的磁性相反,当底部环形永磁体68和顶部环形永磁体69相互吸附式接触时,能够使得异形柱61处于静止状态,从而便于对试管的取放。In order to achieve a closed driving effect for the stem cell test tube, please refer to Figures 1, 2, 3 and 4. It is necessary to set a special-shaped discharge structure 6, which is provided with a test tube placement groove 62 for placing the stem cell test tube and a bottom annular permanent magnet 68 and a top annular permanent magnet 69 for limiting the test tube placement groove 62 to be fixed in the placement position. When taking and placing the stem cell test tube, it is necessary to pull up the limit movable plate 66. At this time, the special-shaped column 61 moves upward, and the upper end surface of the special-shaped column 61 will first be inserted into the interior of the special-shaped taking and placing port 3. Since the structural shape of the circumferential surface of the special-shaped column 61 is consistent with the structural shape of the special-shaped taking and placing port 3, both are plum blossom structures, and the structural dimensions of the circumferential surface of the special-shaped column 61 match the structural dimensions of the special-shaped taking and placing port 3, the circumferential surface of the special-shaped column 61 can achieve the isolation effect of the inner and outer spaces, thereby preventing nitrogen The nitrogen leaks along the moving gap and continues to move upward. Since the height of the limiting connecting column 64 is lower than the height of the special-shaped taking and placing port 3, and the height of the limiting connecting column 64 is higher than the horizontal height of the top of the stem cell test tube after the stem cell test tube is placed in the test tube placement groove 62, the limiting connecting column 64 will first contact the bottom of the disc-shaped cover 71 until the disc-shaped cover 71 is pushed open. At this time, the special-shaped column 61 can take over the sealing function of the disc-shaped cover 71, thereby preventing nitrogen from leaking along the moving gap and reducing the temperature impact on the nitrogen. Since the magnetism of the upper annular end face of the bottom annular permanent magnet 68 is opposite to the magnetism of the lower annular end face of the top annular permanent magnet 69, when the bottom annular permanent magnet 68 and the top annular permanent magnet 69 are in adsorption contact with each other, the special-shaped column 61 can be in a static state, thereby facilitating the taking and placing of the test tube.

关于所述异形放料结构6的具体结构,请参阅图3和图4,包括由铜材料制成的异形柱61和顶部环形永磁体69,为了便于取放试管,需要使得所述异形柱61的高度高于异形取放口3的高度,所述异形柱61在靠近其圆周面的部位设置有多个环形阵列式且用于放置干细胞试管的试管放置槽62,所述异形柱61的底部中心设置有向上凹陷的空心槽63,所述异形柱61的上端面中心设置有限位连接柱64,所述限位连接柱64的顶端固定安装有活动杆65,所述活动杆65的顶端设置有与其一体式结构的限位活动板66,所述异形柱61的底部外围设置有一体式结构且向外围凸起的限位活动环67,所述限位活动环67的上端面和环形嵌入槽5的内部分别嵌入有底部环形永磁体68和顶部环形永磁体69。As for the specific structure of the special-shaped discharge structure 6, please refer to Figures 3 and 4. It includes a special-shaped column 61 made of copper material and a top annular permanent magnet 69. In order to facilitate the placement of test tubes, it is necessary to make the height of the special-shaped column 61 higher than the height of the special-shaped placement port 3. The special-shaped column 61 is provided with a plurality of test tube placement grooves 62 in an annular array for placing stem cell test tubes at a position close to its circumferential surface. The bottom center of the special-shaped column 61 is provided with an upwardly concave hollow groove 63. A limited position connecting column 64 is provided at the center of the upper end surface of the special-shaped column 61. A movable rod 65 is fixedly installed on the top of the limit connecting column 64. A limit movable plate 66 with an integral structure therewith is provided on the top of the movable rod 65. A limit movable ring 67 with an integral structure and protruding toward the periphery is provided on the bottom periphery of the special-shaped column 61. A bottom annular permanent magnet 68 and a top annular permanent magnet 69 are respectively embedded in the upper end surface of the limit movable ring 67 and the inside of the annular embedding groove 5.

为了防止部件在运动过程中造成氮气泄露,请参阅图1、图2、图5和图6,需要设置控压式密封结构7,其内部设置有能够安放于盖槽4内部的盘形盖71以及用于对相对运动间隙进行抵触式封闭的内弹性气膜78和外弹性气膜79,工作时,使用充气设备向环形气体流动腔73的内部充入高压气体,在气体压力的作用下,内弹性气膜78产生向中心的膨胀现象,从而使得内弹性气膜78内表面与活动杆65封闭式接触,防止活动杆65在移动过程中造成的氮气泄露现象的发生,同时,由于所述内弹性气膜78和外弹性气膜79均为具备弹性的橡胶材料制成,且所述内弹性气膜78的弹性强度为所述外弹性气膜79的弹性强度的二倍以上,因此,外弹性气膜79在气体压力的作用下抵触在盖槽4的内壁,从而使得盘形盖71处于密闭状态,防止氮气泄露,进而防止部件在运动过程中造成氮气泄露。In order to prevent nitrogen leakage during the movement of the components, please refer to Figures 1, 2, 5 and 6. A pressure-controlled sealing structure 7 is required, in which a disc-shaped cover 71 that can be placed inside the cover groove 4 and an inner elastic gas membrane 78 and an outer elastic gas membrane 79 for contact-sealing the relative movement gap are provided. When working, a high-pressure gas is filled into the annular gas flow cavity 73 using an inflating device. Under the action of the gas pressure, the inner elastic gas membrane 78 expands toward the center, so that the inner surface of the inner elastic gas membrane 78 is in contact with the active gas flow cavity 73. The movable rod 65 is in closed contact to prevent nitrogen leakage caused by the movable rod 65 during movement. At the same time, since the inner elastic air membrane 78 and the outer elastic air membrane 79 are both made of elastic rubber materials, and the elastic strength of the inner elastic air membrane 78 is more than twice the elastic strength of the outer elastic air membrane 79, the outer elastic air membrane 79 contacts the inner wall of the cover groove 4 under the action of gas pressure, so that the disc-shaped cover 71 is in a closed state to prevent nitrogen leakage, thereby preventing nitrogen leakage caused by the movement of the components.

关于所述控压式密封结构7的具体结构,请参阅图5和图6,包括能够安放于盖槽4内部的盘形盖71,所述盘形盖71的中心设置有用于套放活动杆65的杆孔72,所述盘形盖71的内部设置有环形气体流动腔73,所述盘形盖71在位于所述环形气体流动腔73的内侧设置有连通杆孔72的内环形气体储存腔74,所述盘形盖71在位于所述环形气体流动腔73的外侧设置有连通盘形盖71外圆周面的外环形气体储存腔75,所述环形气体流动腔73和内环形气体储存腔74之间通过多个第一气体流动孔76连通,所述环形气体流动腔73和外环形气体储存腔75之间通过多个第二气体流动孔77连通,所述盘形盖71在位于环形气体流动腔73和杆孔72的交汇处嵌入有内弹性气膜78,所述盘形盖71在位于所述外环形气体储存腔75的圆周边缘部位嵌入有外弹性气膜79,所述盘形盖71中设置有连通环形气体流动腔73且内部安装有气阀的气体注入孔710,所述盘形盖71的内部设置有连通其上端面和环形气体流动腔73的通气口711。As for the specific structure of the pressure-controlled sealing structure 7, please refer to Figures 5 and 6, which include a disc-shaped cover 71 that can be placed inside the cover groove 4, a rod hole 72 for housing the movable rod 65 is provided at the center of the disc-shaped cover 71, an annular gas flow chamber 73 is provided inside the disc-shaped cover 71, an inner annular gas storage chamber 74 connected to the rod hole 72 is provided on the inner side of the annular gas flow chamber 73 of the disc-shaped cover 71, an outer annular gas storage chamber 75 connected to the outer circumferential surface of the disc-shaped cover 71 is provided on the outer side of the annular gas flow chamber 73 of the disc-shaped cover 71, and the annular gas flow chamber 73 and the inner annular gas storage chamber 74 are connected to each other. The annular gas flow chamber 73 and the outer annular gas storage chamber 75 are connected via a plurality of first gas flow holes 76, the annular gas flow chamber 73 and the outer annular gas storage chamber 75 are connected via a plurality of second gas flow holes 77, the disc-shaped cover 71 is embedded with an inner elastic gas membrane 78 at the intersection of the annular gas flow chamber 73 and the rod hole 72, the disc-shaped cover 71 is embedded with an outer elastic gas membrane 79 at the circumferential edge of the outer annular gas storage chamber 75, the disc-shaped cover 71 is provided with a gas injection hole 710 connected with the annular gas flow chamber 73 and having an air valve installed therein, and the disc-shaped cover 71 is provided with an air vent 711 connecting its upper end surface and the annular gas flow chamber 73.

为了实现对最大气压的控制效果,请参阅图1、图2和图7,需要设置最大气压控制结构8,其内部设置有能够对位于控压式密封结构7内部的气体进行堵塞且在向上移动时实现气体向上流动的阀板86以及对阀板86向上的运动产生阻尼效应的螺旋弹簧88,当环形气体流动腔73内部的气压大于螺旋弹簧88的弹性强度时,会使得阀板86向上移动,此时,多余的气体会沿通气口711、进气孔84、气压流动槽87和排气孔85向外排放,从而实现对最大气压的控制效果,防止气压过大造成部件难以移动现象的发生。In order to achieve the control effect of the maximum air pressure, please refer to Figures 1, 2 and 7. It is necessary to set up a maximum air pressure control structure 8, which is internally provided with a valve plate 86 that can block the gas inside the pressure control sealing structure 7 and realize the upward flow of gas when moving upward, and a coil spring 88 that produces a damping effect on the upward movement of the valve plate 86. When the air pressure inside the annular gas flow chamber 73 is greater than the elastic strength of the coil spring 88, the valve plate 86 will move upward. At this time, excess gas will be discharged outward along the vent 711, the air inlet 84, the air pressure flow groove 87 and the exhaust hole 85, thereby achieving the control effect of the maximum air pressure and preventing the occurrence of the phenomenon that the parts are difficult to move due to excessive air pressure.

关于所述最大气压控制结构8的具体结构,请参阅图7,包括纵向空心柱81,所述纵向空心柱81的底端设置有与其一体式结构且固定安装在盘形盖71上端面的底部固定板82,所述纵向空心柱81的内部设置有部件活动空腔83,所述纵向空心柱81的底端设置有连通部件活动空腔83和通气口711的进气孔84,所述纵向空心柱81的顶端设置有连通部件活动空腔83和其上端面的排气孔85,所述纵向空心柱81在位于所述部件活动空腔83的内部安放有能够沿部件活动空腔83纵向活动的阀板86,所述阀板86的边缘设置有气压流动槽87,为了起到必要的气体阻挡作用,需要使得所述阀板86的中心线与所述气压流动槽87之间的间距大于所述进气孔84的结构半径,所述阀板86的上方安放有处于压缩状态的螺旋弹簧88。As for the specific structure of the maximum air pressure control structure 8, please refer to Figure 7, which includes a longitudinal hollow column 81, the bottom end of which is provided with a bottom fixed plate 82 which is an integral structure with the longitudinal hollow column 81 and fixedly mounted on the upper end surface of the disc-shaped cover 71, the interior of the longitudinal hollow column 81 is provided with a component movable cavity 83, the bottom end of the longitudinal hollow column 81 is provided with an air inlet hole 84 connecting the component movable cavity 83 and the air vent 711, the top of the longitudinal hollow column 81 is provided with an exhaust hole 85 connecting the component movable cavity 83 and its upper end surface, the longitudinal hollow column 81 is provided with a valve plate 86 which can move longitudinally along the component movable cavity 83, the edge of the valve plate 86 is provided with an air pressure flow groove 87, in order to play the necessary gas blocking role, it is necessary to make the distance between the center line of the valve plate 86 and the air pressure flow groove 87 greater than the structural radius of the air inlet hole 84, and a coil spring 88 in a compressed state is placed above the valve plate 86.

在使用时,将液氮倒入至液氮储存腔2的内部,且位于液氮储存腔2内部的量需要符合实际需求;When in use, liquid nitrogen is poured into the liquid nitrogen storage chamber 2, and the amount of liquid nitrogen in the liquid nitrogen storage chamber 2 needs to meet actual needs;

向上提拉限位活动板66,此时,异形柱61向上移动,异形柱61的上端面会首先插入至异形取放口3的内部,异形柱61的圆周面能够实现内、外空间的隔绝效果,继续向上运动,会使得限位连接柱64首先抵触在盘形盖71的底部,直至盘形盖71被顶开,此时,异形柱61能够接替盘形盖71的封闭功能,当底部环形永磁体68和顶部环形永磁体69相互吸附式接触时,能够使得异形柱61处于静止状态,从而便于对试管的取放;The limit movable plate 66 is pulled upwards. At this time, the special-shaped column 61 moves upwards. The upper end surface of the special-shaped column 61 will first be inserted into the inside of the special-shaped access opening 3. The circumferential surface of the special-shaped column 61 can achieve the isolation effect of the inner and outer spaces. Continuing to move upwards will make the limit connecting column 64 first contact the bottom of the disc-shaped cover 71 until the disc-shaped cover 71 is pushed open. At this time, the special-shaped column 61 can take over the sealing function of the disc-shaped cover 71. When the bottom annular permanent magnet 68 and the top annular permanent magnet 69 are in adsorption contact with each other, the special-shaped column 61 can be in a static state, thereby facilitating the access to the test tube;

当需要关闭设备时,需要首先将盘形盖71插入至盖槽4的内部,而后向下按压限位活动板66,使得异形柱61伸入至液氮储存腔2的内部,即可。When the device needs to be closed, the disc-shaped cover 71 needs to be inserted into the cover groove 4 first, and then the limiting movable plate 66 is pressed downward to allow the special-shaped column 61 to extend into the liquid nitrogen storage chamber 2.

尽管已经示出和描述了本发明的实施例,对于本领域的普通技术人员而言,可以理解在不脱离本发明的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变型,本发明的范围由所附权利要求及其等同物限定。Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims (10)

1.一种干细胞储存保护提取装置,包括用于储存液氮的液氮储存罐(1)、设置于液氮储存罐(1)内部的液氮储存腔(2)、设置于液氮储存罐(1)顶端中心的异形取放口(3)、设置于异形取放口(3)顶端的盖槽(4)以及设置于异形取放口(3)底部外围的环形嵌入槽(5),其特征在于:还包括,1. A stem cell storage, protection and extraction device, comprising a liquid nitrogen storage tank (1) for storing liquid nitrogen, a liquid nitrogen storage chamber (2) arranged inside the liquid nitrogen storage tank (1), a special-shaped access opening (3) arranged at the top center of the liquid nitrogen storage tank (1), a cover groove (4) arranged at the top of the special-shaped access opening (3), and an annular embedding groove (5) arranged at the periphery of the bottom of the special-shaped access opening (3), characterized in that: it also includes, 异形放料结构(6),其内部设置有用于放置干细胞试管的试管放置槽(62)以及用于限定试管放置槽(62)固定在取放位置的底部环形永磁体(68)和顶部环形永磁体(69);A special-shaped discharge structure (6), wherein a test tube placement groove (62) for placing a stem cell test tube is provided inside the structure, and a bottom annular permanent magnet (68) and a top annular permanent magnet (69) for limiting the test tube placement groove (62) to be fixed at a placement position; 控压式密封结构(7),其内部设置有能够安放于盖槽(4)内部的盘形盖(71)以及用于对相对运动间隙进行抵触式封闭的内弹性气膜(78)和外弹性气膜(79);A pressure-controlled sealing structure (7), wherein a disc-shaped cover (71) capable of being placed inside the cover groove (4) and an inner elastic air film (78) and an outer elastic air film (79) for contact-sealing the relative motion gap are arranged inside the structure; 以及最大气压控制结构(8),其内部设置有能够对位于控压式密封结构(7)内部的气体进行堵塞且在向上移动时实现气体向上流动的阀板(86)以及对阀板(86)向上的运动产生阻尼效应的螺旋弹簧(88)。and a maximum air pressure control structure (8), wherein a valve plate (86) is provided inside the maximum air pressure control structure (8) and is capable of blocking the gas inside the pressure control sealing structure (7) and enabling the gas to flow upward when moving upward, and a coil spring (88) that produces a damping effect on the upward movement of the valve plate (86). 2.根据权利要求1所述的一种干细胞储存保护提取装置,其特征在于:所述异形放料结构(6)包括由铜材料制成的异形柱(61)和顶部环形永磁体(69),所述异形柱(61)在靠近其圆周面的部位设置有多个环形阵列式且用于放置干细胞试管的试管放置槽(62),所述异形柱(61)的底部中心设置有向上凹陷的空心槽(63),所述异形柱(61)的上端面中心设置有限位连接柱(64),所述限位连接柱(64)的顶端固定安装有活动杆(65),所述活动杆(65)的顶端设置有与其一体式结构的限位活动板(66),所述异形柱(61)的底部外围设置有一体式结构且向外围凸起的限位活动环(67),所述限位活动环(67)的上端面和环形嵌入槽(5)的内部分别嵌入有底部环形永磁体(68)和顶部环形永磁体(69)。2. A stem cell storage, protection and extraction device according to claim 1, characterized in that: the special-shaped discharge structure (6) comprises a special-shaped column (61) made of copper material and a top annular permanent magnet (69), the special-shaped column (61) is provided with a plurality of test tube placement grooves (62) in an annular array for placing stem cell test tubes at a position close to its circumferential surface, the bottom center of the special-shaped column (61) is provided with a hollow groove (63) recessed upward, a limit connecting column (64) is provided at the center of the upper end surface of the special-shaped column (61), a movable rod (65) is fixedly installed at the top end of the limit connecting column (64), and a limit movable plate (66) with an integral structure is provided at the top end of the movable rod (65), a limit movable ring (67) with an integral structure and protruding toward the periphery is provided at the bottom periphery of the special-shaped column (61), and the upper end surface of the limit movable ring (67) and the inside of the annular embedding groove (5) are respectively embedded with a bottom annular permanent magnet (68) and a top annular permanent magnet (69). 3.根据权利要求2所述的一种干细胞储存保护提取装置,其特征在于:所述异形柱(61)圆周面的结构外形与所述异形取放口(3)的结构外形一致、均为梅花状结构,且异形柱(61)圆周面的结构尺寸与所述异形取放口(3)的结构尺寸匹配。3. A stem cell storage, protection and extraction device according to claim 2, characterized in that: the structural shape of the circumferential surface of the special-shaped column (61) is consistent with the structural shape of the special-shaped access opening (3), both are plum blossom structures, and the structural dimensions of the circumferential surface of the special-shaped column (61) match the structural dimensions of the special-shaped access opening (3). 4.根据权利要求3所述的一种干细胞储存保护提取装置,其特征在于:所述异形柱(61)的高度高于异形取放口(3)的高度。4. The stem cell storage, protection and extraction device according to claim 3, characterized in that the height of the special-shaped column (61) is higher than the height of the special-shaped taking and placing opening (3). 5.根据权利要求4所述的一种干细胞储存保护提取装置,其特征在于:所述限位连接柱(64)的高度低于异形取放口(3)的高度,且所述限位连接柱(64)的高度高于干细胞试管安放在试管放置槽(62)内部后、干细胞试管顶端的水平高度。5. A stem cell storage, protection and extraction device according to claim 4, characterized in that: the height of the limiting connecting column (64) is lower than the height of the special-shaped access opening (3), and the height of the limiting connecting column (64) is higher than the horizontal height of the top of the stem cell test tube after the stem cell test tube is placed inside the test tube placement groove (62). 6.根据权利要求5所述的一种干细胞储存保护提取装置,其特征在于:所述底部环形永磁体(68)上环形端面的磁性和顶部环形永磁体(69)下环形端面的磁性相反。6. A stem cell storage, protection and extraction device according to claim 5, characterized in that the magnetism of the upper annular end surface of the bottom annular permanent magnet (68) is opposite to the magnetism of the lower annular end surface of the top annular permanent magnet (69). 7.根据权利要求6所述的一种干细胞储存保护提取装置,其特征在于:所述控压式密封结构(7)包括能够安放于盖槽(4)内部的盘形盖(71),所述盘形盖(71)的中心设置有用于套放活动杆(65)的杆孔(72),所述盘形盖(71)的内部设置有环形气体流动腔(73),所述盘形盖(71)在位于所述环形气体流动腔(73)的内侧设置有连通杆孔(72)的内环形气体储存腔(74),所述盘形盖(71)在位于所述环形气体流动腔(73)的外侧设置有连通盘形盖(71)外圆周面的外环形气体储存腔(75),所述环形气体流动腔(73)和内环形气体储存腔(74)之间通过多个第一气体流动孔(76)连通,所述环形气体流动腔(73)和外环形气体储存腔(75)之间通过多个第二气体流动孔(77)连通,所述盘形盖(71)在位于环形气体流动腔(73)和杆孔(72)的交汇处嵌入有内弹性气膜(78),所述盘形盖(71)在位于所述外环形气体储存腔(75)的圆周边缘部位嵌入有外弹性气膜(79),所述盘形盖(71)中设置有连通环形气体流动腔(73)且内部安装有气阀的气体注入孔(710),所述盘形盖(71)的内部设置有连通其上端面和环形气体流动腔(73)的通气口(711)。7. A stem cell storage, protection and extraction device according to claim 6, characterized in that: the pressure-controlled sealing structure (7) comprises a disc-shaped cover (71) that can be placed inside the cover groove (4), the center of the disc-shaped cover (71) is provided with a rod hole (72) for housing the movable rod (65), the inside of the disc-shaped cover (71) is provided with an annular gas flow chamber (73), the disc-shaped cover (71) is provided with an inner annular gas storage chamber (74) connected to the rod hole (72) on the inner side of the annular gas flow chamber (73), the disc-shaped cover (71) is provided with an outer annular gas storage chamber (75) connected to the outer circumferential surface of the disc-shaped cover (71) on the outer side of the annular gas flow chamber (73), the annular gas flow chamber (73) and the inner annular gas storage chamber (74) are provided. The gas storage chambers (74) are connected to each other via a plurality of first gas flow holes (76), the annular gas flow chamber (73) and the outer annular gas storage chamber (75) are connected to each other via a plurality of second gas flow holes (77), the disc-shaped cover (71) is embedded with an inner elastic gas membrane (78) at the intersection of the annular gas flow chamber (73) and the rod hole (72), the disc-shaped cover (71) is embedded with an outer elastic gas membrane (79) at the circumferential edge of the outer annular gas storage chamber (75), the disc-shaped cover (71) is provided with a gas injection hole (710) connected to the annular gas flow chamber (73) and having a gas valve installed therein, and the disc-shaped cover (71) is provided with a vent (711) inside thereof that is connected to its upper end surface and the annular gas flow chamber (73). 8.根据权利要求7所述的一种干细胞储存保护提取装置,其特征在于:所述内弹性气膜(78)和外弹性气膜(79)均为具备弹性的橡胶材料制成,且所述内弹性气膜(78)的弹性强度为所述外弹性气膜(79)的弹性强度的二倍以上。8. A stem cell storage, protection and extraction device according to claim 7, characterized in that: the inner elastic air membrane (78) and the outer elastic air membrane (79) are both made of elastic rubber material, and the elastic strength of the inner elastic air membrane (78) is more than twice the elastic strength of the outer elastic air membrane (79). 9.根据权利要求8所述的一种干细胞储存保护提取装置,其特征在于:所述最大气压控制结构(8)包括纵向空心柱(81),所述纵向空心柱(81)的底端设置有与其一体式结构且固定安装在盘形盖(71)上端面的底部固定板(82),所述纵向空心柱(81)的内部设置有部件活动空腔(83),所述纵向空心柱(81)的底端设置有连通部件活动空腔(83)和通气口(711)的进气孔(84),所述纵向空心柱(81)的顶端设置有连通部件活动空腔(83)和其上端面的排气孔(85),所述纵向空心柱(81)在位于所述部件活动空腔(83)的内部安放有能够沿部件活动空腔(83)纵向活动的阀板(86),所述阀板(86)的边缘设置有气压流动槽(87),所述阀板(86)的上方安放有处于压缩状态的螺旋弹簧(88)。9. A stem cell storage, protection and extraction device according to claim 8, characterized in that: the maximum air pressure control structure (8) comprises a longitudinal hollow column (81), the bottom end of the longitudinal hollow column (81) is provided with a bottom fixing plate (82) which is integrally structured with the longitudinal hollow column (81) and fixedly mounted on the upper end surface of the disc-shaped cover (71), a component movable cavity (83) is provided inside the longitudinal hollow column (81), an air inlet hole (84) connecting the component movable cavity (83) and the vent (711) is provided at the bottom end of the longitudinal hollow column (81), an exhaust hole (85) connecting the component movable cavity (83) and the upper end surface thereof is provided at the top end of the longitudinal hollow column (81), a valve plate (86) which can move longitudinally along the component movable cavity (83) is arranged inside the longitudinal hollow column (81), an air pressure flow groove (87) is provided on the edge of the valve plate (86), and a helical spring (88) in a compressed state is arranged above the valve plate (86). 10.根据权利要求9所述的一种干细胞储存保护提取装置,其特征在于:所述阀板(86)的中心线与所述气压流动槽(87)之间的间距大于所述进气孔(84)的结构半径。10. The stem cell storage, protection and extraction device according to claim 9, characterized in that: the distance between the center line of the valve plate (86) and the air pressure flow groove (87) is greater than the structural radius of the air inlet hole (84).
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