CN118006464A - Portable cell hypoxia culture device - Google Patents
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention relates to the technical field of cell culture, in particular to a portable cell hypoxia culture device which comprises a pre-mixing air tank fixedly arranged in a cabinet body, wherein a main metering pump is communicated with the pre-mixing air tank. The portable cell hypoxia culture device provided by the invention can be used for rapidly adjusting the culture chamber to the environment required by cell culture, so that the cells can be transported among different fixed cell culture devices, and the cells can be continuously cultured in the transportation process, when the portable cell hypoxia culture device is used, a culture dish is transported to the fixed cell culture device from the fixed cell culture device, then the fixed cell culture device is transported to a target position and then taken out of the culture dish, a large number of unnecessary steps can be saved compared with the traditional cell culture and transportation process, the problem that the traditional cell hypoxia culture device cannot be transported conveniently in the culture process is solved, the time consumption for transporting the cells can be effectively reduced, and the cell culture quality is ensured.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a portable cell hypoxia culture device.
Background
Cell culture refers to a method for enabling cells to survive, grow and reproduce normally in an in vitro simulated environment, and is also called a cell cloning technology. In the cell culture process, a hypoxia environment of cells in an in-vivo environment is generally required to be simulated, and the corresponding gas component proportion, temperature and humidity conditions and the like are required.
The existing cell low-temperature culture device generally integrates a large number of functions in order to adapt to the demands of different cell cultures, uses a large-sized air tank to supply air, and meanwhile, has large equipment volume and large weight, and can only be used at a fixed position in a laboratory generally so as to be incapable of being carried. However, the cells to be cultured often need to be transported in a short distance between laboratories in different areas, but these cells cannot be exposed to air for a long time, which results in that the cells generally need to be stored and transported by freezing after the cell culture is completed, and then the cells are restored to activity by a series of means after reaching the destination.
Therefore, a portable cell hypoxia culture device is needed to solve the problem that the conventional cell hypoxia culture equipment cannot be transported conveniently in the culture process, effectively reduce the time consumption of transporting cells and ensure the cell culture quality.
Disclosure of Invention
The application aims to solve the problem that the conventional cell hypoxia culture equipment cannot be transported conveniently in the culture process, and provides a portable cell hypoxia culture device which can effectively reduce the time consumption of transporting cells and ensure the cell culture quality.
In order to achieve the above purpose, the present invention provides the following technical solutions: a portable cell hypoxia culture device comprising:
The device comprises a cabinet body, wherein a premixing air tank is fixedly arranged in the cabinet body, and a main metering pump is communicated with the premixing air tank;
The culture chamber is fixed in the cabinet body, the front end of the culture chamber is sealed through a front door, a clamping device used for fixing the culture dish is arranged in the culture chamber, a third sensor group used for monitoring the gas components, the air pressure and the temperature and humidity state in the culture chamber is arranged in the culture chamber, and an exhaust pump is communicated with the culture chamber;
The air exchange chamber is arranged below the culture chamber and connected with the culture chamber through a sealing device, the bottom of the air exchange chamber is fixedly provided with a bottom push rod, the output end of the bottom push rod is driven with a bracket for supporting a culture dish, the output end of the main metering pump is respectively communicated with the culture chamber and the air exchange chamber through an air distribution valve, and the air exchange chamber is communicated with a one-way exhaust valve;
The mixing device comprises a gas mixing box fixed with the culture chamber, the inside of the gas mixing box is respectively communicated with the inside of the culture chamber through a suction pump and a blowing pump, oxygen, nitrogen and carbon dioxide gases are respectively and quantitatively supplied in the gas mixing box through a gas supply device, a first heater is fixed in the gas mixing box, and a first sensor group for monitoring the gas composition, the gas pressure and the temperature and humidity state in the gas mixing box is fixed in the gas mixing box;
The controller is fixed at the top of the cabinet body and is electrically connected with the main metering pump, the third sensor group, the exhaust pump, the bottom push rod, the distributing valve, the suction pump, the blowing pump, the first heater and the first sensor group respectively.
Preferably, the clamping device comprises a pair of clamping push rods fixedly mounted at the left end and the right end of the culture chamber, the output end of each clamping push rod is provided with a clamping arm used for clamping the culture dish, and the clamping push rods are electrically connected with the controller.
Preferably, the upper wall surface of the culture chamber is fixedly provided with a top camera for observing the top condition of the culture dish, and the bottom wall surface and the side wall of the culture chamber are provided with bottom cameras for observing the bottom and side conditions of the culture dish, and the top cameras and the bottom cameras are electrically connected with the controller.
Preferably, the sealing device comprises a connecting frame fixed between the air exchange chamber and the culture chamber, an adjusting push rod is fixedly arranged at the bottom of the culture chamber, and a sealing plate inserted into the connecting frame to seal and isolate the culture chamber from the air exchange chamber is driven at the output end of the adjusting push rod.
Preferably, the top of the cabinet body is fixedly provided with a display electrically connected with the controller, the cultivation room is internally and fixedly provided with a level sensor electrically connected with the controller and used for detecting levelness of the cultivation room, and the cabinet body is internally provided with an energy storage device electrically connected with the controller.
Preferably, a second heater for heating the mixed gas in the premixed gas tank is fixedly installed on the premixed gas tank, a temperature detection sensor for detecting the temperature of the mixed gas in the premixed gas tank is fixedly installed on the premixed gas tank, a second sensor group for monitoring the gas composition, the air pressure and the temperature and humidity state in the ventilation chamber is fixedly installed on the ventilation chamber, and the second heater, the temperature detection sensor and the second sensor group are respectively and electrically connected with the controller.
Preferably, a stirring device is arranged in the gas mixing box, the stirring device comprises a motor fixedly installed inside the gas mixing box, and the output end of the motor is driven by a blade for mixing gas inside the gas mixing box.
Preferably, the inlet end of the air suction pump is connected with an air suction control valve, the inlet end of the air suction control valve is connected with at least three air suction branch pipes distributed at different positions in the culture chamber, the outlet end of the air blowing pump is connected with an air blowing control valve, the outlet end of the air blowing control valve is connected with at least three air blowing branch pipes distributed at different positions in the culture chamber, and the air suction control valve and the air blowing control valve are electrically connected with a controller.
Preferably, the air supply device comprises at least three unit tanks which are arranged in the cabinet body and are respectively used for supplying oxygen, nitrogen and carbon dioxide, each unit tank is respectively communicated with the air mixing box through an independent unit metering valve, and each unit metering valve is electrically connected with the controller.
Preferably, a pull handle is fixedly arranged outside the cabinet body, and a glass window for directly observing the culture dish is arranged on the front door.
Compared with the prior art, the invention has the beneficial effects that:
The portable cell hypoxia culture device provided by the invention can be used for rapidly adjusting the culture chamber to the environment required by cell culture, so that the cells can be transported among different fixed cell culture devices, and the cells can be continuously cultured in the transportation process, when the portable cell hypoxia culture device is used, a culture dish is transported to the fixed cell culture device from the fixed cell culture device, then the fixed cell culture device is transported to a target position and then taken out of the culture dish, a large number of unnecessary steps can be saved compared with the traditional cell culture and transportation process, the problem that the traditional cell hypoxia culture device cannot be transported conveniently in the culture process is solved, the time consumption for transporting the cells can be effectively reduced, and the cell culture quality is ensured.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention.
Fig. 2 is a schematic side view of the present invention.
Fig. 3 is a cross-sectional view of the structure of the present invention.
FIG. 4 is a schematic view showing the internal structure of the culture chamber of the present invention.
Fig. 5 is a schematic diagram showing the connection of the air supply device and the mixing device according to the present invention.
FIG. 6 is a schematic illustration of the structural connection of the premix tank and the culture chamber of the invention.
Fig. 7 is a schematic view showing the internal structure of the mixing device of the present invention.
FIG. 8 is a schematic diagram showing the connection of the aeration chamber and the culture chamber according to the present invention.
In the figure: 1. a cabinet body; 2. a mixing device; 201. a getter pump; 202. a first heater; 203. a gas mixing box; 204. a first sensor group; 205. an air blowing pump; 3. a stirring device; 301. a paddle; 302. a motor; 4. a closing device; 401. a connecting frame; 402. adjusting the push rod; 403. a closing plate; 5. a gas supply device; 501. a unit metering valve; 502. a unit tank; 6. a clamping device; 601. a clamping arm; 602. clamping the push rod; 7. a top camera; 8. a bottom push rod; 9. a second sensor group; 10. an energy storage device; 11. a pull handle; 12. a display; 13. a controller; 14. a front door; 15. a culture chamber; 16. a one-way exhaust valve; 17. an air suction branch pipe; 18. a blowing branch pipe; 19. an air suction control valve; 20. a blow control valve; 21. a second heater; 22. a premix gas tank; 23. a temperature detection sensor; 24. a main metering pump; 25. an air distributing valve; 26. a third sensor group; 27. a culture dish; 28. a bracket; 29. a level sensor; 30. a bottom camera; 31. a ventilation chamber; 32. an exhaust pump.
Detailed Description
In order to make the objects, technical solutions, and advantages of the present invention more apparent, the embodiments of the present invention will be further described in detail with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are some, but not all, embodiments of the present invention, are intended to be illustrative only and not limiting of the embodiments of the present invention, and that all other embodiments obtained by persons of ordinary skill in the art without making any inventive effort are within the scope of the present invention.
In the description of the present invention, it should be noted that the terms "center," "middle," "upper," "lower," "left," "right," "inner," "outer," "top," "bottom," "side," "vertical," "horizontal," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate description of the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "a," an, "" the first, "" the second, "" the third, "" the fourth, "" the fifth, "and the sixth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
For purposes of brevity and description, the principles of the embodiments are described primarily by reference to examples. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the embodiments. It will be apparent, however, to one of ordinary skill in the art that the embodiments may be practiced without limitation to these specific details. In some instances, well-known methods and structures have not been described in detail so as not to unnecessarily obscure the embodiments. In addition, all embodiments may be used in combination with each other.
In a first embodiment, referring to fig. 1 to 8, the present invention provides a technical solution: a portable cell hypoxia culture device comprising:
referring to fig. 1,2, 3, 4, 6 and 8, a premixed gas tank 22 is fixedly installed in the cabinet 1, a main metering pump 24 is communicated with the premixed gas tank 22, and mixed gas in the premixed gas tank 22 is supplied in advance by an external gas supply device and is formed by mixing the mixed gas according to the ratio of oxygen, nitrogen, carbon dioxide and other gases required to culture cells;
Referring to fig. 3,4, 5, 6 and 8, a culture chamber 15 is fixed in the cabinet 1, the front end of the culture chamber 15 is sealed by a front door 14, a clamping device 6 for fixing a culture dish 27 is arranged in the culture chamber 15, the clamping device 6 comprises a pair of clamping push rods 602 fixedly arranged at the left end and the right end of the culture chamber 15, the output end of each clamping push rod 602 is driven with a clamping arm 601 for clamping the culture dish 27, the clamping push rods 602 are electrically connected with a controller 13, a third sensor group 26 for monitoring the gas composition, the air pressure and the temperature and humidity state in the culture chamber 15 is arranged in the culture chamber 15, an exhaust pump 32 is communicated with the culture chamber 15, the exhaust pump 32 is used for exhausting the gas in the culture chamber 15 to the outside of the device, an operator places the culture dish 27 on the clamping arms 601 by opening the front door 14 and clamps the culture dish 27 by the clamping arms 601;
Referring to fig. 3,4 and 8, the ventilation chamber 31 is disposed below the culture chamber 15, the ventilation chamber 31 is connected with the culture chamber 15 through a sealing device 4, the sealing device 4 comprises a connecting frame 401 fixed between the ventilation chamber 31 and the culture chamber 15, an adjusting push rod 402 is fixedly installed at the bottom of the culture chamber 15, a sealing plate 403 inserted into the connecting frame 401 to seal the culture chamber 15 and the ventilation chamber 31 is driven at the output end of the adjusting push rod 402, the sealing state of the sealing plate 403 to seal the culture chamber 15 and the ventilation chamber 31 is changed by changing the sealing plate 403 in different working positions of the connecting frame 401 through the adjusting push rod 402, a bottom push rod 8 is fixedly installed at the bottom of the ventilation chamber 31, a bracket 28 for supporting the culture dish 27 is driven by the bottom push rod 8 to move up and down, the culture dish 27 is enabled to perform position conversion between the ventilation chamber 31 and the culture chamber 15, the ventilation chamber 31 are respectively communicated with the output end of a main metering pump 24 through the distributing valve 25, the main metering pump 24 can respectively send the mixed gas in the culture chamber 15 and the ventilation chamber 31 to the ventilation chamber 31 through the distributing valve 25 and the air outlet valve 16, and the ventilation valve 16 is respectively arranged in the ventilation chamber 31 is arranged in the ventilation chamber 31, and the ventilation valve 16 is arranged in the ventilation chamber 31;
Referring to fig. 3,5, 6 and 7, the mixing device 2 includes a mixing box 203 fixed with the culture chamber 15, the interior of the mixing box 203 is respectively communicated with the interior of the culture chamber 15 through a suction pump 201 and a blowing pump 205, oxygen, nitrogen and carbon dioxide gases are respectively and quantitatively supplied into the mixing box 203 through a gas supply device 5, a first heater 202 is fixed in the mixing box 203, a first sensor group 204 for monitoring the gas composition, the gas pressure and the temperature and humidity state in the mixing box 203 is fixed in the mixing box 203, an inlet end of the suction pump 201 is connected with a suction control valve 19, an inlet end of the suction control valve 19 is connected with at least three suction branch pipes 17 distributed at different positions in the culture chamber 15, an outlet end of the blowing pump 205 is connected with a blowing control valve 20, at least three blowing branch pipes 18 distributed at different positions in the culture chamber 15 are electrically connected with a controller 13, the suction control valve 19 and the blowing control valve 20 are respectively, the gas supply device 5 includes at least three metering units 502 which are respectively arranged in the cabinet 1 and are respectively connected with the respective metering units 502, each metering unit 502 is respectively and each metering unit 502 is respectively connected with the respective metering units 502, each metering unit 502 is conveniently connected with each of the respective metering units 502, if other gases are needed to be used for cells to be cultured, the number of the unit tanks 502 is increased as required, and corresponding gases are stored, so that the corresponding gases are conveniently supplied into the mixed gas box 203, the mixing device 2 performs gas regulation work in a manner that firstly the volume of the culture chamber 15 is fixed, so that the quantity occupied by each gas in the current gas can be calculated only by detecting the air pressure in the culture chamber 15 and the proportion of each gas, then the composition proportion of all the gases is changed by quantitatively adding the corresponding gases, specifically, the mixing device 2 performs three stages of exhausting, supplementing and mixing when the gas regulation work is performed, during the exhausting stage, the air pump 32 discharges the gases positioned in the culture chamber 15 to enable the air pressure in the culture chamber 15 to be reduced to four fifths of the preset value, when the air pressure is required to be reduced to four fifths of the preset value, the operator can change the other proportion at this time, for example, the air pump 32 can be adjusted to be reduced to a value of ninety five percent of the preset air pressure, then enters the supplementing stage, the controller calculates the composition proportion of each gas, during the current gas is filled into the supplementing stage, the supplementing gas is calculated, the required proportion of each gas is filled into the culture chamber 203, the mixed gas box 203 is sucked into the mixed gas box 203 when the mixed gas box is filled with the mixed gas box 203 after the required to be quantitatively, the mixed gas box is filled into the mixed gas box 203, and the mixed gas box is mixed with the mixed gas box is filled into the mixed gas box after the mixed gas box 203, and the mixed gas is mixed with the mixed gas box is filled into the culture chamber after the air is mixed gas box 203, and the mixed gas is filled into the air tank is filled into the culture box 203, and the air into the air tank is heated. The gas in the gas mixing box 203 is re-sent into the culture chamber 15 through the gas blowing branch pipe 18 while mixing by the gas blowing pump 205, so that circulation is formed, the gas adjusting work can be completed once until the gas components in the gas mixing box 203 and the culture chamber 15 are detected to be the same through the first sensor group 204 and the third sensor group 26, when the proportion of the gas components in the culture chamber 15 cannot be adjusted to a preset value through the gas adjusting work once, the gas adjusting work is repeatedly carried out by the mixing device 2, so that the gas components in the culture chamber 15 are kept in a state close to the preset proportion all the time, and the temperature of the gas is adjusted through the first heater 202 while the gas adjusting work is carried out by the mixing device 2;
The controller 13, please refer to fig. 1,3, 4,5, 6, 7 and 8, the controller 13 is fixed at the top of the cabinet 1, and the controller 13 is respectively electrically connected with the main metering pump 24, the third sensor group 26, the exhaust pump 32, the bottom push rod 8, the air distribution valve 25, the suction pump 201, the air blowing pump 205, the first heater 202 and the first sensor group 204, the top of the cabinet 1 is fixedly provided with the display 12 electrically connected with the controller 13, the display 12 is used for displaying various working parameters of the controller 13, the operator can conveniently adjust the working state of the controller 13, the level sensor 29 electrically connected with the controller 13 and used for detecting the levelness of the culture chamber 15 is fixedly arranged in the culture chamber 15, the level sensor 29 is used for detecting the levelness of the culture chamber 15, so as to avoid the components such as to be spilled out of the culture medium in the culture dish 27 due to the inclination of the culture chamber 15, the operator carries the equipment in a proper way, the cabinet 1 is internally provided with the energy storage device 10 electrically connected with the controller 13, the energy storage device 10 can be an electric device or the lithium battery 10 can be an electric device or the electric device is directly arranged in the whole glass container 11, the electric device is arranged in the glass container 11, and the whole glass container is directly arranged in the drawer 11 is convenient to be used for carrying the external equipment, the handle 11 is arranged on the drawer 11, the drawer 11 is convenient to be used for carrying the external equipment, the drawer 11 is directly is arranged;
Referring to fig. 4, 6 and 8, a second heater 21 for heating the mixed gas inside the premixed gas tank 22 is fixedly installed on the premixed gas tank 22, a temperature detection sensor 23 for detecting the temperature of the mixed gas inside the premixed gas tank 22 is fixedly installed on the premixed gas tank 22, a second sensor group 9 for monitoring the gas composition, the air pressure and the temperature and humidity state inside the ventilation chamber 31 is fixedly installed on the ventilation chamber 31, and the second heater 21, the temperature detection sensor 23 and the second sensor group 9 are respectively electrically connected with the controller 13.
When the device is used, an operator firstly supplements corresponding gas into the premixing air tank 22 and each unit tank 502 through an external gas supply device, then places the culture dish 27 with the prepared culture material from the front door 14 onto the clamping arm 601 and closes the front door 14, then opens the controller 13 to start a culture procedure, the bottom push rod 8 pushes the bracket 28 below the culture dish 27, then the clamping push rod 602 drives the clamping arm 601 to the non-working position, then the culture dish 27 is sent into the ventilation chamber 31 by the bracket 28 under the driving of the bottom push rod 8, then the sealing device 4 seals the culture chamber 15 and the ventilation chamber 31 respectively, then the premixing air tank 22 supplies mixed gas into the ventilation chamber 31 through the main metering pump 24, when the air pressure in the ventilation chamber 31 reaches a specified value, the one-way exhaust valve 16 starts to work and discharges the redundant gas in the gas exchange chamber 31, so that the gas originally in the gas exchange chamber 31 is extruded into the gas exchange chamber 31 by utilizing the mixed gas, the gas in the culture chamber 15 is pumped out to a designated vacuum degree by the exhaust pump 32, then the mixed gas is replenished into the culture chamber 15 by the main metering pump 24, when the gas in the culture chamber 15 is replenished, and the other components in the gas exchange chamber 31 reach a preset value, the sealing device 4 is opened, the bottom push rod 8 sends the culture dish 27 into the culture chamber 15 again, at the moment, the culture dish 27 is clamped by the clamping arm 601, the bracket 28 is retracted into the gas exchange chamber 31, then the culture chamber 15 and the gas exchange chamber 31 are sealed by the sealing device 4 again, and then the gas in the culture chamber 15 can be subjected to periodical gas adjustment work by utilizing the mixing device 2, thereby meeting the environmental requirements of cell culture.
In the present invention, since many equipments are installed in the culture chamber 15, the space in the culture chamber 15 is much larger than that of the ventilation chamber 31, so if it takes a long time to directly adjust the gas composition of the culture dish 27 just put in the culture chamber 15 by the mixing device 2, the gas adjustment can be completed only by a period of half an hour or more, while by the present invention, the culture dish 27 is put in the ventilation chamber 31 to be adjusted, the gas composition in the ventilation chamber 31 can be made to approach the gas ratio required for the preset cell culture within three minutes, and the separate adjustment can also avoid the culture dish 27 from being in an environment unsuitable for culture such as a low pressure environment, and simultaneously the gas composition adjustment in the culture chamber 15 can be completed within three minutes by directly evacuating the culture chamber 15 by the exhaust pump 32 and then directly filling the corresponding already prepared mixed gas by the pre-mixed gas tank 22, in this way, the culture dish 27 can be in a preset proper environment again only for a maximum of three minutes, the culture efficiency is greatly improved, the trouble that the problem is caused because the cells are in an improper environment for a long time is avoided, the integrated level of the device is high, the volume is small, a large amount of gas is not required to be stored in the premixed gas tank 22 and the unit tank 502, only the gas which is required to be consumed in short-distance transportation is required to be stored, the weight of the gas which is required to be stored in the transportation time within one hour is not more than ten kilograms, and the high-pressure storage is not required, the tank body with low strength and low weight can be used for both the premixed gas tank 22 and the unit tank 502, so that the whole quality of the cell hypoxia culture device can be controlled to be less than one hundred kilograms, and can be carried by two operators, meanwhile, the continuous culture of cells is not delayed in the carrying process, so that the use is more convenient. Solves the problem that the conventional cell hypoxia culture equipment cannot be transported conveniently in the culture process, can effectively reduce the time consumption of transporting cells and ensure the cell culture quality.
In the second embodiment, referring to fig. 3 and fig. 4, on the basis of the first embodiment, the top camera 7 for observing the top condition of the culture dish 27 is fixedly installed on the upper wall surface of the culture chamber 15, and the bottom camera 30 for observing the bottom and side conditions of the culture dish 27 is arranged on the bottom wall surface and the side wall of the culture chamber 15, the top camera 7 and the bottom camera 30 are electrically connected with the controller 13, and on the premise that an operator does not open the front door 14 by installing the top camera 7 and the bottom camera 30, the images acquired by the top camera 7 and the bottom camera 30 are displayed by the display 12, so that real-time attention is paid to the culture state in the culture dish 27, the problem that the culture process is caused due to the fact that the transportation or the gas is not regulated in place is avoided, but the problem that the treatment is not paid attention in time is solved, the problem that the conventional cell hypoxia culture equipment cannot be transported conveniently in the culture process is further solved, the time consumption of transferring cells can be effectively reduced, and the cell culture quality is guaranteed.
In the third embodiment, referring to fig. 7, on the basis of the second embodiment, the stirring device 3 is disposed in the air mixing box 203, the stirring device 3 includes a motor 302 fixedly installed inside the air mixing box 203, and the output end of the motor 302 is driven with a blade 301 for mixing the air inside the air mixing box 203, so that the efficiency of mixing the air in the air mixing box 203 can be further improved through the stirring device 3, the time required by the mixing device 2 for one period of adjustment of the air in the culture chamber 15 is shortened, the adjustment efficiency is improved, the problem that the conventional cell hypoxia culture equipment cannot be transported conveniently in the culture process is further solved, the time consumption for transporting cells can be effectively reduced, and the cell culture quality is guaranteed.
The invention also provides a use method of the portable cell hypoxia culture device based on the fourth embodiment, which comprises the following steps:
Step one: the operator first supplements the corresponding gas into the premix tank 22 and each of the unit tanks 502 through the external gas supply device, then puts the culture dish 27, which has been prepared with the culture material, from the front door 14 onto the holding arm 601 and closes the front door 14, then opens the controller 13 to start the culture process, at which time the bottom push rod 8 pushes the bracket 28 under the culture dish 27, then the holding push rod 602 drives the holding arm 601 to the inactive position, then the culture dish 27 is sent into the ventilation chamber 31 by the bracket 28 under the driving of the bottom push rod 8, then the closing device 4 seals the culture chamber 15 and the ventilation chamber 31, respectively, then the premix tank 22 supplies the mixed gas into the ventilation chamber 31 through the main metering pump 24, when the gas pressure in the ventilation chamber 31 reaches a specified value, the one-way exhaust valve 16 starts to work and discharges redundant gas in the gas exchange chamber 31, so that the gas originally in the gas exchange chamber 31 is extruded into the gas exchange chamber 31 by utilizing mixed gas, meanwhile, the gas in the culture chamber 15 is pumped out to a specified vacuum degree by the exhaust pump 32, then the mixed gas is replenished into the culture chamber 15 by the main metering pump 24, when the gas in the culture chamber 15 is replenished, and other components in the gas exchange chamber 31 reach a preset value, the sealing device 4 is opened, the bottom push rod 8 sends the culture dish 27 into the culture chamber 15 again, at the moment, the culture dish 27 is clamped by the clamping arm 601, the bracket 28 is retracted into the gas exchange chamber 31, and then the culture chamber 15 and the gas exchange chamber 31 are sealed by the sealing device 4 again;
Step two: after the culture chamber 15 is resealed by the sealing device 4, the mixing device 2 starts to perform gas regulation on the culture chamber 15, firstly, the gas in the culture chamber 15 is discharged through the discharge pump 32 so that the gas pressure in the culture chamber 15 is reduced to four fifths of a preset value, then the discharge pump 32 is closed, at the moment, the controller 13 calculates the quantity occupied by each gas in the current gas, then calculates the quantity required to supplement when the gas pressure in the culture chamber 15 is restored to a set value and the various gases are kept to be in a preset proportion after being supplemented, and then quantitatively supplements the gas through the gas supply device 5, at the moment, the suction pump 201 sucks the gas in the culture chamber 15 into the gas mixing box 203 by utilizing the suction branch pipe 17 positioned in the culture chamber 15 and mixes the gas supplemented in the gas mixing box 203, and at the same time, the gas blowing pump 205 can send the gas in the gas mixing box 203 into the culture chamber 15 again through the gas blowing branch pipe 18 so as to form a circulation until the same gas components in the gas mixing box 203 and the culture chamber 15 are detected through the first sensor group 204 and the third sensor group 26, and the gas components in the culture chamber 15 are kept in a preset proportion, and the gas regulation period is completed, and the gas components in the culture chamber 15 can be stably maintained within a preset gas regulation period range;
step three: when it is necessary to take out the dish 27 again, the operator gives an instruction through the controller 13, at which time the mixing apparatus 2 stops the gas conditioning operation, closes the blowing pump 205, and pumps the gas in the culture chamber 15 into the gas mixing box 203 by means of the suction pump 201 until the gas pressure in the culture chamber 15 decreases to a preset value, and these mixed gases pumped into the gas mixing box 203 are available for the next use, and then the suction pump 201 is turned off, and the controller 13 notifies the operator that the front door 14 can be opened to take out the dish 27.
While the foregoing describes illustrative embodiments of the present application so that those skilled in the art may understand the present application, the present application is not limited to the specific embodiments, and all applications and creations utilizing the inventive concepts are within the scope of the present application as long as the modifications are within the spirit and scope of the present application as defined and defined in the appended claims to those skilled in the art.
Claims (10)
1. A portable cell hypoxia culture device comprising:
The novel intelligent cabinet temperature control device comprises a cabinet body (1), wherein a premixing air tank (22) is fixedly arranged in the cabinet body (1), and a main metering pump (24) is communicated with the premixing air tank (22);
The culture device comprises a culture chamber (15), wherein the culture chamber (15) is fixed in a cabinet body (1), the front end of the culture chamber (15) is sealed through a front door (14), a clamping device (6) for fixing a culture dish (27) is arranged in the culture chamber (15), a third sensor group (26) for monitoring the gas composition, the air pressure and the temperature and humidity state in the culture chamber (15) is arranged in the culture chamber (15), and an exhaust pump (32) is communicated with the culture chamber (15);
The air exchange chamber (31) is arranged below the culture chamber (15), the air exchange chamber (31) is connected with the culture chamber (15) through a sealing device (4), a bottom push rod (8) is fixedly arranged at the bottom of the air exchange chamber (31), a bracket (28) for supporting a culture dish (27) is driven at the output end of the bottom push rod (8), the output end of the main metering pump (24) is respectively communicated with the culture chamber (15) and the air exchange chamber (31) through an air distribution valve (25), and a one-way exhaust valve (16) is communicated with the air exchange chamber (31);
the mixing device (2), the mixing device (2) comprises a gas mixing box (203) fixed with the culture chamber (15), the inside of the gas mixing box (203) is respectively communicated with the inside of the culture chamber (15) through a suction pump (201) and a blowing pump (205), oxygen, nitrogen and carbon dioxide gas are respectively and quantitatively supplied in the gas mixing box (203) through a gas supply device (5), a first heater (202) is fixed in the gas mixing box (203), and a first sensor group (204) for monitoring the gas composition, the gas pressure and the temperature and humidity state in the gas mixing box (203) is fixed in the gas mixing box (203);
The controller (13), the top at cabinet body (1) is fixed to controller (13), and controller (13) respectively with main metering pump (24), third sensor group (26), exhaust pump (32), bottom push rod (8), distributing valve (25), aspirator pump (201), blowing pump (205), first heater (202) and first sensor group (204) electric connection.
2. The portable cell hypoxia culture device of claim 1, wherein: the clamping device (6) comprises a pair of clamping push rods (602) fixedly mounted at the left end and the right end of the culture chamber (15), clamping arms (601) used for clamping the culture dish (27) are driven at the output end of each clamping push rod (602), and the clamping push rods (602) are electrically connected with the controller (13).
3. The portable cell hypoxia culture device of claim 1, wherein: the upper wall surface of the culture chamber (15) is fixedly provided with a top camera (7) for observing the top condition of the culture dish (27), and bottom cameras (30) for observing the bottom and side conditions of the culture dish (27) are arranged on the bottom wall surface and the side wall of the culture chamber (15), and the top camera (7) and the bottom cameras (30) are electrically connected with the controller (13).
4. The portable cell hypoxia culture device of claim 1, wherein: the sealing device (4) comprises a connecting frame (401) fixed between the air exchange chamber (31) and the culture chamber (15), an adjusting push rod (402) is fixedly arranged at the bottom of the culture chamber (15), and a sealing plate (403) inserted into the connecting frame (401) and used for sealing and isolating the culture chamber (15) and the air exchange chamber (31) is driven at the output end of the adjusting push rod (402).
5. The portable cell hypoxia culture device of claim 1, wherein: the top of the cabinet body (1) is fixedly provided with a display (12) which is electrically connected with a controller (13), the inside of the culture chamber (15) is fixedly provided with a level sensor (29) which is electrically connected with the controller (13) and is used for detecting the levelness of the culture chamber (15), and the inside of the cabinet body (1) is provided with an energy storage device (10) which is electrically connected with the controller (13).
6. The portable cell hypoxia culture device of claim 1, wherein: the gas-exchange device is characterized in that a second heater (21) for heating mixed gas in the gas-exchange tank (22) is fixedly arranged on the gas-exchange tank (22), a temperature detection sensor (23) for detecting the temperature of the mixed gas in the gas-exchange tank (22) is fixedly arranged on the gas-exchange chamber (31), a second sensor group (9) for monitoring the gas composition, the gas pressure and the temperature and humidity state in the gas-exchange chamber (31) is fixedly arranged on the gas-exchange chamber (31), and the second heater (21), the temperature detection sensor (23) and the second sensor group (9) are respectively electrically connected with the controller (13).
7. The portable cell hypoxia culture device of claim 1, wherein: the mixing device is characterized in that a stirring device (3) is arranged in the gas mixing box (203), the stirring device (3) comprises a motor (302) fixedly installed inside the gas mixing box (203), and a blade (301) for mixing gas inside the gas mixing box (203) is driven by the output end of the motor (302).
8. The portable cell hypoxia culture device of claim 1, wherein: the inlet end of the suction pump (201) is connected with a suction control valve (19), the inlet end of the suction control valve (19) is connected with at least three suction branch pipes (17) distributed at different positions in the culture chamber (15), the outlet end of the air blowing pump (205) is connected with an air blowing control valve (20), the outlet end of the air blowing control valve (20) is connected with at least three air blowing branch pipes (18) distributed at different positions in the culture chamber (15), and the suction control valve (19) and the air blowing control valve (20) are electrically connected with the controller (13).
9. The portable cell hypoxia culture device of claim 1, wherein: the air supply device (5) comprises at least three unit tanks (502) which are arranged in the cabinet body (1) and are used for supplying oxygen, nitrogen and carbon dioxide respectively, each unit tank (502) is communicated with the air mixing box (203) through an independent unit metering valve (501), and each unit metering valve (501) is electrically connected with the controller (13).
10. The portable cell hypoxia culture device of claim 1, wherein: the outside of the cabinet body (1) is fixedly provided with a pull handle (11), and the front door (14) is provided with a glass window for directly observing the culture dish (27).
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