CN117849326A - Liquid path system, in-vitro diagnosis equipment and use method thereof - Google Patents

Abstract

The invention provides a liquid path system, in-vitro diagnosis equipment and a use method thereof, wherein the liquid path system comprises a plurality of mutually independent mixing channels, each mixing channel comprises a valve, a pump and a dripper, and the valve comprises a liquid outlet and at least two liquid inlets; in the mixing channel, a liquid outlet is sequentially connected with a pump and a dripper in series; each liquid inlet can be independently switched to communicate with or isolate from the liquid outlet and one of the liquid inlets is connected to a liquid carrier container loaded with reagent and the other liquid inlet is connected to a liquid carrier container loaded with cleaning liquid. The liquid path system has self-cleaning capability when being applied to in-vitro diagnostic equipment, can keep extremely low blockage rate, and reduces the possibility of blockage.

Description

Liquid path system, in-vitro diagnosis equipment and use method thereof

Technical Field

The invention belongs to the technical field of medical appliances, and particularly relates to a liquid path system, in-vitro diagnosis equipment and a use method thereof.

Background

The immunoblotting detection method is a hybridization technology combining high-resolution gel electrophoresis and immunochemistry analysis technology, is the most commonly used method for detecting protein characteristics, expression and distribution at present, and has the advantages of large analysis capacity, high sensitivity, strong specificity and the like. The immunoblotting detection analyzer is an instrument for automatically completing the operations of reagent addition, incubation, cleaning, drying, judgment and the like according to the test steps of the immunoblotting detection method.

In the immunoblotting detector, a pump is used to dispense a liquid such as a reagent or a cleaning liquid. The immunoblotting detector in the prior art is provided with a plurality of independent liquid channels according to the types of reagents in the compatible reagent kit, for example, when the types of reagents are 3, 4 liquid channels are provided, one of the liquid channels is a cleaning liquid channel 10, and the remaining 3 liquid channels are reagent channels, namely, a first reagent channel 20a, a second reagent channel 20b and a third reagent channel 20c (as shown in fig. 1). The cleaning liquid channel 10 includes a cleaning pump 11 and a cleaning liquid droplet head 12 connected to an outlet end of the cleaning pump 11, and an inlet end of the cleaning pump 11 is connected to a cleaning liquid container 31. Each of the reagent channels includes a reagent pump 21 and a reagent dropper 22 connected to an outlet end of the reagent pump 21, and an inlet end of the reagent pump 21 is adapted to be connected to a reagent container 32 (as shown in fig. 1 and 2), wherein the reagent container 32 connected to the reagent pump 21 of the first reagent channel 20a contains a reagent 1, the reagent container 32 connected to the reagent pump 21 of the second reagent channel 20b contains a reagent 2, and the reagent container 32 connected to the reagent pump 21 of the third reagent channel 20c contains a reagent 3. When the immunoblotting detector is used for performing immunoblotting detection analysis operation, at least the following steps are performed:

step S10, dripping the reagent 1 to the immunoblotting detection membrane strip by utilizing the first reagent channel 20 a;

step S20, incubating the immunoblotting detection membrane strip; the duration of the step is determined according to the kit and is generally 20-60 min;

step S30, adding the cleaning solution to the immunoblotting detection membrane strip by using the cleaning solution channel 10 to elute the reagent 1 on the immunoblotting detection membrane strip;

step S40, adding reagent 2 to the immunoblotting detection membrane strip by using the second reagent channel 20 b;

step S50, incubating the immunoblotting detection membrane strip; the duration of the step is determined according to the kit and is generally 20-60 min;

step S60, adding the cleaning solution to the immunoblotting detection membrane strip by using the cleaning solution channel 10 to elute the reagent 2 on the immunoblotting detection membrane strip;

step S70, adding the wash solution to the immunoblotting detection membrane strip by using the third reagent channel 20 c;

step S80, incubating the immunoblotting detection membrane strip; the duration of the step is determined according to the kit and is generally 20-60 min;

step S90, adding the cleaning solution to the immunoblotting detection membrane strip by using the cleaning solution channel 10 to elute the reagent 3 on the immunoblotting detection membrane strip.

Typically, after the entire detection process is completed, the operator manually replaces each reagent vessel 32 with the cleaning liquid vessel 31, and sequentially pumps the cleaning liquid in the cleaning liquid vessel with the pumps 21 of each reagent channel to clean each of the reagent channels.

In the above flow, after the step S10 is completed, the reagent 1 remains in the first reagent channel 20a, after the step S40 is completed, the reagent 2 remains in the second reagent channel 20b, and after the step S70 is completed, the reagent 3 remains in the third reagent channel 20 c. After the whole detection flow is finished, the residual reagents of various reagents in the corresponding actual channels are long, for example, the residual time of the reagent 1 in the first reagent channel 20a is as long as 2-3 hours, which leads to the drying and adhesion of the reagents in the corresponding reagent channels, and the cleaning is difficult; if the operator does not clean all of the reagent channels after the entire test procedure is completed, the sticking phenomenon is more serious. For a long time, each reagent channel, especially the reagent dropper 22, was blocked and not used properly.

Disclosure of Invention

The invention aims to provide a liquid path system, an in-vitro diagnosis device and a use method thereof, and the in-vitro diagnosis device with the liquid path system can timely clean a pipeline when in use, so as to avoid blockage.

To achieve the above object, the present invention provides a fluid circuit system for an in vitro diagnostic device, comprising a plurality of mixing channels independent of each other, each of said mixing channels comprising a valve, a pump and a dripper, said valve comprising one fluid outlet and at least two fluid inlets;

in the mixing channel, the liquid outlet is sequentially connected with the pump and the dripper in series; each of the liquid inlets can be independently switched to communicate with or isolate from the liquid outlets, and one of the liquid inlets is for connection to a liquid carrier container loaded with a reagent and the other liquid inlet is for connection to a liquid carrier container loaded with a cleaning liquid.

Optionally, the liquid path system further includes a cleaning liquid channel independent of any one of the mixing channels, the cleaning liquid channel including a pump and a dripper connected to each other, and an inlet end of the pump being for connection with the liquid carrying container loaded with the cleaning liquid.

Optionally, the valve comprises a three-way valve.

Optionally, the fluid path system further comprises a controller communicatively connected to all of the valves and the pumps and configured to control opening or closing of each of the fluid inlets and each of the valves.

Optionally, the pump has a first mode of operation and a second mode of operation;

when any one of the pumps performs the first operation mode, the pump is used for sucking the liquid in the liquid carrying container communicated with the pump to the corresponding mixing channel; when any one of the pumps performs the second mode of operation, the pump is operable to deliver liquid in the corresponding mixing channel to the liquid-carrying container in communication therewith.

Optionally, the pump is a peristaltic pump or a plunger pump.

To achieve the above object, the present invention also provides an in vitro diagnostic device comprising an incubation mechanism for carrying a test sample and a fluid path system as described above for sucking a liquid in the carrier fluid container and dripping the liquid to the test sample.

Optionally, the in-vitro diagnostic device further comprises a plurality of said liquid carrying containers, one part of which is used for carrying said reagents and the other part of which is used for carrying said cleaning liquid.

Optionally, the in vitro diagnostic device comprises any one of an immunoblotting detector, a biochemical analyzer, a blood cell analyzer, a chemiluminescent analyzer, and an enzyme linked immunosorbent assay.

In order to achieve the above object, the present invention further provides a method for using the in-vitro diagnostic device as described above, wherein the liquid inlet connected to the liquid carrier container for carrying the reagent in the mixing channel is a reagent inlet, and the liquid inlet connected to the liquid carrier container for carrying the cleaning liquid is a cleaning agent inlet; the use method comprises the following operation for each mixing channel according to a preset reagent adding sequence:

opening the reagent inlet and controlling the pump to aspirate the reagent to the dripper and to drip to the test sample;

closing the reagent inlet;

opening the cleaning liquid inlet, controlling the pump to suck the cleaning liquid to the dripper, and dripping the cleaning liquid to the detection sample to elute the reagent on the detection sample;

closing the cleaning liquid inlet;

after closing the reagent inlet and before opening the cleaning agent inlet, the method of use further comprises: and controlling the start of the incubation mechanism to incubate the detection sample.

Compared with the prior art, the liquid path system, the in-vitro diagnosis equipment and the use method thereof have the following advantages:

the liquid path system comprises a plurality of mutually independent mixing channels, wherein each mixing channel comprises a valve, a pump and a dripper; in the mixing channel, the valve comprises one liquid outlet and at least two liquid inlets; the liquid outlet is sequentially connected with the pump and the dripper in series; each of the liquid outlets can be independently switched to communicate with or be isolated from the liquid outlet, and one of the liquid inlets is for connection to a liquid carrier container loaded with a reagent and the other liquid inlet is for connection to a liquid carrier container loaded with a cleaning liquid. The fluid circuit system is applied to the in-vitro diagnostic device, wherein the fluid inlet connected to the carrier fluid container loaded with the reagent is referred to as a reagent inlet, and the fluid inlet connected to the carrier fluid container loaded with the cleaning fluid is referred to as a cleaning fluid inlet. In actual operation, the following operations are performed for each of the mixing channels according to a predetermined reagent addition sequence: firstly, opening the reagent inlet, and controlling the pump to suck the reagent to the dripper and drop the reagent to a detection sample; then closing the reagent inlet and incubating the detection template; subsequently, opening the cleaning liquid inlet, controlling the pump to suck the cleaning liquid to the dripper, and dripping the cleaning liquid to the detection sample to elute the reagent on the detection template; and finally closing the cleaning liquid inlet. In each mixing channel, the cleaning liquid and the reagent share the liquid outlet, the pump, the dripper, and the pipe between the liquid outlet and the pump, and the pipe between the pump and the dripper in the mixing channel, so that the residual reagent in the liquid outlet, the pump, the dripper, and the pipe between the liquid outlet and the pump, and the pipe between the pump and the dripper is washed during the elution of the detection sample by the cleaning liquid, and the problem of mixing channel blockage caused by long-time residue, drying, and sticking of the reagent is avoided. After the test is completed, an operator does not need to manually replace the liquid carrier container loaded with the reagent with the liquid carrier container loaded with the cleaning liquid, so that the workload of the operator is reduced.

Drawings

The drawings are included to provide a better understanding of the invention and are not to be construed as unduly limiting the invention. Wherein:

FIG. 1 is a schematic diagram of a liquid path system of a prior art immunoblotting detector;

FIG. 2 is a schematic diagram of a reagent channel of a liquid path system of an immunoblotting detector in the prior art;

FIG. 3 is a schematic diagram of a fluid circuit system according to an embodiment of the present invention;

fig. 4 is a schematic diagram of a mixing channel of a liquid path system according to an embodiment of the present invention.

Reference numerals are described as follows:

10-cleaning liquid channel, 11-cleaning pump, 12-cleaning liquid droplet head, 20 a-first reagent channel, 20 b-second reagent channel, 20 c-third reagent channel, 21-reagent pump, 22-reagent droplet head, 31-cleaning liquid container, 32-reagent container;

1000-liquid path system, 1100-mixing channel, 1110-valve, 1111-liquid outlet, 1112-liquid inlet, 1120-first pump, 1130-first dripper, 1200-cleaning liquid channel, 1210-second pump, 1220-second dripper, 2100-liquid carrying container.

Detailed Description

Other advantages and effects of the present invention will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present invention with reference to specific examples. The invention may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present invention. It should be noted that, the illustrations provided in the present embodiment merely illustrate the basic concept of the present invention by way of illustration, and only the components related to the present invention are shown in the drawings and are not drawn according to the number, shape and size of the components in actual implementation, and the form, number and proportion of the components in actual implementation may be arbitrarily changed, and the layout of the components may be more complex.

In addition, each embodiment of the following description has one or more features, respectively, which does not mean that the inventor must implement all features of any embodiment at the same time, or that only some or all of the features of different embodiments can be implemented separately. In other words, those skilled in the art can implement some or all of the features of any one embodiment or a combination of some or all of the features of multiple embodiments selectively, depending on the design specifications or implementation requirements, thereby increasing the flexibility of the implementation of the invention where implemented as possible.

As used in this specification, the singular forms "a", "an" and "the" include plural referents, unless the content clearly dictates otherwise. As used in this specification, the term "or" is generally employed in its sense including "and/or" unless the content clearly dictates otherwise, and the terms "mounted," "connected," and "connected" are to be construed broadly, as for example, they may be fixed, they may be removable, or they may be integrally connected. Either mechanically or electrically. Can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. Relational terms such as first, second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions, nor does it indicate or imply relative importance or number of technical features indicated. It is to be understood that the terms "center," "longitudinal," "transverse," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," "circumferential," and the like, as indicated by the azimuth or positional relationship shown in the drawings, are merely for convenience of description and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular azimuth, be configured and operated in a particular azimuth, and therefore should not be construed as limiting the invention. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.

The invention aims to provide a liquid path system which can be applied to in-vitro diagnostic equipment and has a self-cleaning function, so that the liquid path system has extremely low blockage rate, can be effectively kept unobstructed, and reduces the workload of instrument operators.

The invention will be further described in detail with reference to the accompanying drawings, in order to make the objects, advantages and features of the invention more apparent. It should be noted that the drawings are in a very simplified form and are all to a non-precise scale, merely for convenience and clarity in aiding in the description of embodiments of the invention. The same or similar reference numbers in the drawings refer to the same or similar parts.

Fig. 3 shows a schematic diagram of a fluid circuit system 1000 according to an embodiment of the invention. Referring to fig. 1, the fluid path system 1000 includes a plurality of mixing channels 1100 independent of each other. Fig. 4 shows a schematic view of the mixing channels 1100, as shown in fig. 3, 4, each of the mixing channels 1100 comprising a valve 1110, a pump and a dripper. In the mixing channel 1100, the pump is referred to as a first pump 1120 and the dripper is referred to as a first dripper 1130.

The valve 1110 includes a liquid outlet 1111 and at least two liquid inlets 1112. The liquid outlet 1111 is connected in series with the first pump 1120 and the first dripper 1130 via a pipeline. Each of the liquid inlets 1112 can be independently switched, and when the liquid inlet 1112 is open, the open liquid inlet 1112 communicates with the liquid outlet 1111 and thus with the first pump 1120, and when the liquid inlet 1112 is closed, the closed liquid inlet 1112 is isolated from the liquid outlet 1111 and thus for isolation by the first pump 1120.

The fluid circuit system 1000 may be applied to an in vitro diagnostic device (not shown in the drawings) including, but not limited to, any one of an immunoblotting detector, a biochemical analyzer, a blood cell analyzer, a chemiluminescent analyzer, an enzyme linked immunosorbent analyzer.

In actual use, one of the liquid inlets 1112 in each of the mixing channels 1100 is connected to the liquid carrier container 2100 loaded with a reagent, and the other liquid inlet 1112 is connected to the liquid carrier container 2100 loaded with a cleaning liquid, so that the liquid path system 1000 can add the reagent and the cleaning liquid to a test sample (not shown) of the in vitro diagnostic device. Typically, after one of the reagents is added to the test sample using the fluid path system 1000, the cleaning agent is added to the test sample using the fluid path system 1000 at a time interval. When the cleaning solution is added to the detection sample, the liquid path system 1000 provided in the embodiment of the present invention may further flush the liquid outlet 1111 of the valve 1110 of the corresponding mixing channel 1100 and all parts on the distal end side of the liquid outlet 1111, so as to realize a self-cleaning function, and avoid the problem that the area is blocked due to long-term retention, drying and adhesion of the reagent in the liquid outlet 1111 and all parts on the distal end side of the liquid outlet 1111. Further, after the completion of the entire inspection process, it is not necessary to manually replace the reagent-containing carrier liquid container 2100 with the cleaning liquid carrier liquid container 2100 by an operator after the completion of the inspection process, as in the related art, thereby reducing the workload of the operator. It should be noted that, the "distal side of the liquid outlet 1111" referred to herein refers to a side of the liquid outlet 1111 away from the liquid inlet 1112, and thus all components of the "distal side" include the first pump 1120, the first dripper 1130, the pipeline between the liquid outlet 1111 and the first pump 1120, and the pipeline between the first pump 1120 and the first dripper 1130.

Next, the in-vitro diagnostic device is taken as an immunoblotting detector, and the detection sample is taken as an immunoblotting detection membrane strip as an example for detailed description.

The immunoblotting detector further comprises an incubation mechanism (not shown) for carrying the immunoblotting detection membrane strip, as known to the person skilled in the art. In the present document, the liquid inlet 1112 connected to the liquid carrier 2100 loaded with the reagent in the mixing channel 1100 is referred to as a reagent inlet, and the liquid inlet 1112 connected to the liquid carrier 2100 loaded with the cleaning liquid is referred to as a cleaning liquid inlet. Those skilled in the art will appreciate that the reagent types loaded in the carrier liquid containers 2100 to which the reagent inlets of the different mixing channels 1100 are connected are different.

When performing immunoblotting detection using the immunoblotting detector, the following operations are sequentially performed for each mixing channel 1100 at least in a predetermined reagent addition order:

step S1, the reagent inlet is opened, and the first pump 1120 is controlled to pump the reagent to the first dripper 1130, and the reagent is dripped to the immunoblotting detection membrane strip until the dripped dosage reaches a predetermined dosage.

And S2, closing the reagent inlet.

Step S3, opening the cleaning solution inlet, controlling the first pump 1120 to pump the cleaning solution to the first dripper 1130, and dripping the cleaning solution onto the immunoblotting detection membrane strip to elute the reagent on the immunoblotting detection membrane strip.

And S4, closing the cleaning liquid inlet.

After the step S2 is performed and before the step S3 is performed, a step S5 should be performed, where the step S5 includes controlling the start of the incubation mechanism to incubate the immunoblotting detection membrane strip.

The types of the reagents are described as three examples. The three reagents are numbered as reagent 1, reagent 2 and reagent 3 according to the order of addition. Correspondingly, the number of the mixing channels 1100 is also three, namely a first mixing channel, a second mixing channel and a third mixing channel. Wherein the reagent inlet of the first mixing channel is connected to the liquid carrier container 2100 loaded with the reagent 1, the reagent inlet of the second mixing channel is connected to the liquid carrier container 2100 loaded with the reagent 2, and the reagent inlet of the third mixing channel is connected to the liquid carrier container 2100 loaded with the reagent 3.

Therefore, the operation in performing immunoblotting detection using the immunoblotting detector including the three mixing channels 1100 is as follows: the step S1 and the step S2 are firstly executed on the first mixing channel, then the step S5 is executed, and then the step S3 and the step S4 are executed on the first mixing channel; then, the step S1 and the step S2 are executed for the second mixing channel, then the step S5 is executed, and the step S3 and the step S4 are executed for the second mixing channel; then, the step S1 and the step S2 are performed on the third mixing channel, then the step S5 is performed, and finally the step S3 and the step S4 are performed on the third mixing channel.

As is clear from the above-described flow, in the process of performing the step S3 on the first mixing channel, the liquid outlet 1111 and the components on the distal end side thereof in the first mixing channel have been actually cleaned, so that the remaining reagent 1 therein is discharged without being left for a long time, thereby avoiding the remaining reagent 1 from clogging the liquid outlet 1111 and the components on the distal end side thereof in the first mixing channel. Similarly, in the process of executing the step S3 on the second mixing channel, the liquid outlet 1111 and the components on the far end side thereof in the second mixing channel are also cleaned, so that the residual reagent 2 is discharged, and the residual reagent 2 is prevented from blocking the liquid outlet 1111 and the components on the far end side thereof in the second mixing channel. And, when the step S3 is performed on the third mixing channel, the liquid outlet 1111 and its components on the far end side in the third mixing channel are also cleaned, so that the remaining reagent 3 therein is discharged, and the remaining reagent 3 is prevented from blocking the liquid outlet 1111 and its components on the far end side in the third mixing channel.

Note that, in the above flow, the opening and closing of the reagent inlet and the cleaning solution inlet are mainly described, and the opening and closing of the first pump 1120 is not specifically described, and in fact, when the reagent inlet or the cleaning solution inlet is closed, the first pump 1120 may be closed synchronously, or the first pump 1120 may not be closed.

Preferably, the valve 1110 is a three-way valve, such that the valve 1110 includes two of the liquid inlets. In addition, in some implementations, the cleaning liquid inlets of different of the mixing channels are connected to different carrier liquid containers, which are each loaded with the cleaning liquid, i.e. the cleaning liquid inlet of each of the mixing channels is connected to one carrier liquid container (not shown in the figures) loaded with the cleaning liquid. In other implementations, the wash liquid inlets of different of the mixing channels 1100 are connected to the same carrier liquid container 2100 loaded with the wash liquid, which has the advantage that the number of carrier liquid containers 2100 arranged in the immunoblotting detector is greatly reduced, which is advantageous for reducing the volume of the immunoblotting detector. In addition, in the case where the cleaning liquid inlets of different mixing channels 1100 are connected to the same carrier liquid container 2100 loaded with the cleaning liquid, a plurality of mixing channels 1100 are independent from each other, and it may be understood that a plurality of mixing channels 1100 are connected in parallel to each other.

Preferably, the first pump 1120 has a first mode of operation and a second mode of operation. When the first pump 1120 performs the first operation mode, the first pump 1120 pumps the liquid in the liquid carrier container 2100, which is in communication therewith, to the corresponding mixing channel 1100. When the first pump 1120 performs the second operation mode, the first pump 1120 delivers the liquid in the corresponding mixing channel 1100 to the liquid-carrying container 2100 in communication therewith.

Therefore, it is preferable that a step S01 is further included between the step S1 and the step S2, and the step S01 includes controlling the first pump 1120 to transfer a part of the reagent in the mixing channel 1100 to the carrier liquid container 2100 loaded with the reagent. That is, the configuration of the first pump 1120 enables the mixing channel 1100 to have a recycling function, so that the part of the reagent can be recycled, the waste of the reagent is reduced, and the detection cost is reduced. It will be appreciated that a step S02 may be further included between the step S3 and the step S4, the step S02 including controlling the first pump 1120 to deliver a portion of the cleaning liquid in the mixing channel 1100 to the liquid-carrying container 2100 loaded with the cleaning liquid.

In practice, the first pump 1120 may be a peristaltic pump or a plunger pump, which has high control accuracy, and is beneficial to improving the dosage accuracy of the reagent or the cleaning solution added to the immunoblotting detection membrane strip.

Optionally, as shown in fig. 3, the liquid path system 1000 further includes a cleaning liquid channel 1200 independent of any of the mixing channels 1100. The cleaning liquid channel 1200 includes a pump called a second pump 1210 and a dripper called a second dripper 1220 in the cleaning liquid channel 1200. The inlet end of the second pump 1210 is configured to be connected to the liquid carrier container 2100 loaded with the cleaning liquid, and the inlet end of the second dripper 1220 is connected to the outlet end of the second pump 1210.

The wash liquid channel 1200 is a redundant configuration that controls the second pump 1210 to pump the wash liquid to the second drip head 1220 and drip to the immunoblotting detection membrane strip to elute the reagent on the immunoblotting detection membrane strip when the wash liquid inlet of one of the mixing channels 1100 fails to open.

The second pump 1220 may have the same configuration as the first pump 1120. In other words, the second pump 1220 may be a peristaltic pump or a plunger pump.

Optionally, the fluid circuit system 1000 further includes a controller (not shown). The controller is communicatively connected to all of the valves 1110, all of the first pumps 1120, and the second pumps 1220, and is configured to control the opening and closing of the liquid inlets 1111, the first pumps 1120, and the second pumps 1220 of the valves 1110 according to a set program. Thereby, the degree of automation of the fluid path system 1000 can be improved.

Further, the embodiment of the invention also provides an in-vitro diagnosis device, which is the in-vitro diagnosis device.

Optionally, the in-vitro diagnostic device further comprises a plurality of said carrier liquid containers 2100, wherein one part of said carrier liquid containers 2100 is used for loading said reagents, different said carrier liquid containers 2100 are loaded with different said reagents, and another part of said carrier liquid containers 2100 is used for loading said cleaning liquid. The reagent inlet of each of the mixing channels 1100 is connected to one of the carrier liquid containers 2100 loaded with the reagent, and the wash liquid inlet of each of the mixing channels 1100 and the inlet end of the second pump 1220 of the wash liquid channel 1200 are connected to one of the carrier liquid containers 2100 loaded with the wash liquid. Preferably, only one of the liquid-carrying containers 2100 is used to carry the cleaning liquid, and all of the cleaning liquid inlets of the mixing channel 1100 and the inlet ends of the second pump 1220 are connected to this liquid-carrying container 2100 carrying the cleaning liquid.

Still further, an embodiment of the present invention further provides a method for using an in vitro diagnostic device, where the method at least includes sequentially performing the steps S1, S2, S3 and S4 described above on the plurality of mixing channels 1100 according to a predetermined reagent addition sequence. The using method further comprises the step of executing the step S5 after each execution of the step S2 and before executing the step S3. The use method further comprises the step S01.

Furthermore, as those skilled in the art will appreciate, there may be a gas (typically air) in the fluid path system 1000, and therefore, before the reagent and the wash liquid are added to the immunoblotting detection membrane strip, the air in the corresponding channels should be evacuated to ensure that the dosage of the reagent and the wash liquid added to the immunoblotting detection membrane strip is accurate.

Thus, the operation of controlling the first pump 1120 to pump the reagent to the first dripper 1130 in the step S1 and dripping to the immunoblotting detection membrane strip specifically includes step S11 and step S12. Wherein the step S11 includes controlling the first pump 1120 to suck the reagent to the first dripper 1130 such that the reagent fills a pipe between the liquid carrying container 2100 loaded with the reagent and the reagent inlet, the liquid outlet, a pipe between the liquid outlet and the first pump 1120, a pipe between the first pump 1120 and the first dripper 1130, and the first dripper 1130 to discharge the gas in these components. It should be appreciated that the reagent dropped from the first dripper 1130 is collected in a waste container without being dropped onto the immunoblotting detection membrane strip during the execution of step S11. The step S12 includes controlling the first pump 1120 to continue sucking the reagent to the first dripper 1130 and dripping the reagent to the immunoblotting detection membrane strip.

Similarly, in the step S3, the operation of controlling the first pump 1120 to suck the cleaning liquid to the first dripper 1130 and dripping the cleaning liquid to the immunoblotting detection membrane strip specifically includes a step S31 and a step S32. Wherein the step S31 includes controlling the first pump 1120 to suck the cleaning liquid to the first dripper 1130 such that the cleaning liquid fills a pipe between the carrier liquid container 2100 loaded with the cleaning liquid and the cleaning liquid inlet, the liquid outlet, a pipe between the liquid outlet and the first pump 1120, a pipe between the first pump 1120 and the first dripper 1130, and the first dripper 1130 to discharge the gas in these components. It should be understood that, in the process of performing step S31, the cleaning solution dropped from the first dripper 1130 is collected in a waste liquid container without being dripped to the immunoblotting detection membrane strip. The step S32 includes controlling the first pump 1120 to continue sucking the cleaning liquid to the first dripper 1130 and dripping the reagent to the immunoblotting detection membrane strip.

And the operation of controlling the second pump 1210 to suck the cleaning liquid to the second dripper 1220 and to drip the cleaning liquid to the immunoblotting detection membrane strip specifically includes controlling the second pump 1210 to suck the cleaning liquid to the second dripper 1220 and to fill the cleaning liquid channel to discharge the gas in the cleaning liquid channel, during which the cleaning liquid dripping from the second dripper 1220 is collected to the waste liquid container without dripping to the immunoblotting detection membrane strip. Thereafter, the second pump 1210 is controlled to pump the cleaning solution to the second dripper 1220, and drop the cleaning solution onto the immunoblotting detection membrane strip.

Although the present invention is disclosed above, it is not limited thereto. Various modifications and alterations of this invention may be made by those skilled in the art without departing from the spirit and scope of this invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (10)

1. A fluid path system for an in vitro diagnostic device, comprising a plurality of mixing channels independent of each other, each of said mixing channels comprising a valve, a pump and a dripper;

in the mixing channel, the valve comprises a liquid outlet and at least two liquid inlets, and the liquid outlet is sequentially connected with the pump and the dripper in series; each of the liquid inlets can be independently switched to communicate with or isolate from the liquid outlets, and one of the liquid inlets is for connection to a liquid carrier container loaded with a reagent and the other liquid inlet is for connection to a liquid carrier container loaded with a cleaning liquid.

2. The fluid path system of claim 1, further comprising a cleaning fluid channel independent of any of the mixing channels, the cleaning fluid channel comprising a pump and a dripper connected to each other, and an inlet end of the pump for connection with the liquid carrying container loaded with the cleaning fluid.

3. The fluid path system according to claim 1 or 2, wherein the valve comprises a three-way valve.

4. The fluid circuit system of claim 1 or 2, further comprising a controller in communication with all of said valves and said pumps and configured to control the opening or closing of each of said fluid inlets and each of said valves.

5. The fluid circuit system of claim 1 or 2, wherein the pump has a first mode of operation and a second mode of operation;

when any one of the pumps performs the first operation mode, the pump is used for sucking the liquid in the liquid carrying container communicated with the pump to the corresponding mixing channel; when any one of the pumps performs the second mode of operation, the pump is operable to deliver liquid in the corresponding mixing channel to the liquid-carrying container in communication therewith.

6. The fluid path system according to claim 1 or 2, wherein the pump is a peristaltic pump or a plunger pump.

7. An in vitro diagnostic device comprising an incubation mechanism for carrying a test sample and a fluid path system according to any one of claims 1-6 for aspirating the liquid in the carrier fluid container and dripping the liquid to the test sample.

8. The in-vitro diagnostic device according to claim 7, further comprising a plurality of said liquid carrier containers, one part of said liquid carrier containers being for carrying said reagents and another part of said liquid carrier containers being for carrying said cleaning liquid.

9. The in vitro diagnostic device according to claim 8, wherein said in vitro diagnostic device comprises any one of an immunoblotting detector, a biochemical analyzer, a blood cell analyzer, a chemiluminescent analyzer, an enzyme linked immunosorbent analyzer.

10. A method of using the in vitro diagnostic device according to any one of claims 7 to 9, wherein said liquid inlet in said mixing channel connected to said liquid carrier container carrying said reagent is a reagent inlet and said liquid inlet connected to said liquid carrier container carrying said cleaning fluid is a cleaning agent inlet; wherein the use method comprises the following operations for each of the mixing channels in a predetermined reagent addition sequence:

opening the reagent inlet and controlling the pump to aspirate the reagent to the dripper and to drip to the test sample;

closing the reagent inlet;

opening the cleaning liquid inlet, controlling the pump to suck the cleaning liquid to the dripper, and dripping the cleaning liquid to the detection sample to elute the reagent on the detection sample;

closing the cleaning liquid inlet;

after closing the reagent inlet and before opening the wash inlet, the method of use further comprises: and controlling the start of the incubation mechanism to incubate the detection sample.