CN117838909A - Antibacterial and anti-inflammatory dressing, and preparation method and application thereof - Google Patents

Abstract

An antibacterial and anti-inflammatory dressing, a preparation method and application thereof, wherein: the antibacterial and anti-inflammatory dressing is formed by stacking and felting nano fibers, the nano fibers comprise a core tube and a sheath film coated outside the core tube, the sheath film has a lace-shaped reticular porous structure and contains nano metal antibacterial agents, and the core tube contains hemostatic or anti-inflammatory drugs; the preparation method comprises the steps of preparing sheath fluid and core tube fluid, preparing nanofiber felt and treating the surface of nanofiber felt; can be used as wound plaster or wound sterilizing and antiinflammatory or sterilizing and antiinflammatory hemostatic nursing pad. The nanofiber well simulates the structure and function of natural skin, has certain hydrophilicity, can better meet the nursing and healing requirements of complex wound environments, has high specific surface area, can keep good mechanical properties, has antibacterial efficacy and hemostatic or anti-inflammatory effects, can effectively control the release of medicines, reduces the harm of toxic and side effects, realizes multiple applications, and promotes wound healing.

Description

Antibacterial and anti-inflammatory dressing, and preparation method and application thereof

Technical Field

The invention relates to a dressing and a preparation method and application thereof, in particular to an antibacterial and anti-inflammatory dressing and a preparation method and application thereof, and belongs to the technical field of production and manufacture of medical consumables and application thereof.

Background

Skin wound is the tearing or breakage of skin caused by external injury, which can lead to blood loss and even shock, uncontrollable bleeding and wound infection are important causes of death of wounded, so rapid hemostasis of wound is an important link for treatment of wounded.

When acute wounds, such as cuts, wounds, etc., develop, they trigger the body's own mechanisms of hemostasis, inflammation, cell proliferation and tissue remodeling, and any abnormality in the healing stage may lead to the formation of chronic wounds. In contrast to acute wounds, chronic wounds do not heal in a short period of time following physiological mechanisms, showing more pronounced protease activity, lower growth factor activity and more pro-inflammatory cytokines, wounds are susceptible to infection and thus show a sustained abnormal inflammatory response, and wound dressings are a better choice for controlling and managing chronic wounds.

Wound infection is an obstacle to normal healing of wounds, and chronic wounds are susceptible to bacteria and microorganisms causing infection leading to inflammation and even sepsis. Wounds covered by common dressings are also infected, thus prolonging the inflammatory response, while wound dressings with antimicrobial activity are effective in preventing wound infection.

The conventional wound dressing is generally composed of materials and structural materials such as hydrogel, a film, sponge, foam and the like, has poor water permeability and air permeability, and is not beneficial to wound healing.

The nanofiber is a relatively new wound dressing material, has a plurality of intersecting grids as small as a few micrometers to hundreds of nanometers, can provide extremely large exposed surface area and nano-porosity, can more effectively permeate water and oxygen and better exchange nutrient substances and remove metabolic wastes compared with the conventional wound dressing, and can also enhance the hemostatic function; most importantly, the broad surface area of the nanofiber dressing can also be used to effectively load/incorporate drugs as compared to conventional gauze and nonwoven fabrics, thereby becoming a bioactive dressing.

The method for producing nanofibers includes a method for producing nanofibers by using an electrospinning technique, for example, an invention patent application of "method for producing nanofibers" (application number: 200880122530.6), an invention patent application of "method for producing nanofibers by electrospinning collagen aqueous solution" (application number: 201210355709.4), an invention patent of "method for producing a PEO-containing collagen-based nanofiber membrane" (grant bulletin number: CN 105568559B), an invention patent of "method for producing polyacrylonitrile-based porous hollow carbon fibers by coaxial electrospinning" (grant bulletin number: CN 102691136B), and the like.

The nanofiber obtained by adopting the electrostatic spinning technology has unique structure and biological function, and can provide ideal microenvironment for cell proliferation, migration and differentiation.

However, the single-layer fiber dressing obtained by adopting the existing electrostatic spinning technology is difficult to meet the nursing and healing requirements of the complex wound environment, and the prepared fiber membrane has the problems of overlarge fiber stacking density, overlarge pore diameter and the like due to the smaller diameter of the electrostatic spinning nano fiber, so that the development of the electrostatic spinning medical dressing is influenced; meanwhile, the medicines are simply added into the electrospun nanofiber, and toxic and side effects can be generated due to repeated administration caused by explosive large-dose release of the medicines and repeated replacement of the dressing, so that the antibacterial and anti-inflammatory dressing with ideal treatment effect is required to be obtained, and intensive research and development are still required.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention particularly provides an antibacterial and anti-inflammatory dressing, a preparation method and application thereof, and aims at:

the medical dressing with the antibacterial and anti-inflammatory functions is provided for wound healing, the structure and the function of natural skin are simulated as much as possible, the nursing and healing requirements of the complex wound environment are met, the higher specific surface area of the nanofiber is created, the good mechanical properties of the nanofiber are maintained, and the occurrence of toxic and side effects is effectively controlled; meanwhile, the requirements of simple preparation method, low production cost and the like are met, so that the method has good application prospect and high use value.

In order to achieve the above purpose, the invention firstly provides an antibacterial and anti-inflammatory dressing, which has the technical scheme that:

an antibacterial and anti-inflammatory dressing is formed by stacking and felting nanofibers, wherein the nanofibers comprise a core tube and a sheath film coated outside the core tube, the sheath film has a lace net-shaped porous structure, the sheath film contains a nano metal antibacterial agent, and the core tube contains a hemostatic drug or an anti-inflammatory drug.

Further, the core tube and the sheath film are respectively formed by combining a water-insoluble polymer and a water-soluble polymer and an alcohol-soluble polymer.

Optionally, the water-insoluble polymer is polyacrylonitrile, and the water-soluble and alcohol-soluble polymer is polyethylene glycol or polyvinylpyrrolidone.

Further, the weight ratio of the water-insoluble polymer to the water-soluble and alcohol-soluble polymer is 0.6:1 or 1:0.6.

Optionally, the hemostatic drug is tranexamic acid, and the anti-inflammatory drug is any one of gentamicin sulfate, tetracycline hydrochloride, ibuprofen or ciprofloxacin.

Optionally, the nano metal antibacterial agent is a silver ion antibacterial agent.

Secondly, the invention also provides a preparation method of the antibacterial and anti-inflammatory dressing, which specifically comprises the following steps:

a preparation step of sheath fluid, a preparation step of core tube fluid, a preparation step of nanofiber felt and a surface treatment step of nanofiber felt, wherein:

the preparation steps of the sheath fluid comprise:

respectively measuring or weighing nano metal antibacterial agent, water-insoluble polymer and water-soluble and alcohol-soluble polymer, then respectively adding into N, N-dimethylformamide, heating and stirring until the nano metal antibacterial agent, the water-insoluble polymer and the water-soluble and alcohol-soluble polymer are completely dissolved to obtain a sheath fluid, and then pouring the sheath fluid into a sheath fluid reservoir;

the preparation steps of the core tube fluid comprise:

respectively measuring or weighing a water-insoluble polymer and a water-soluble polymer and an alcohol-soluble polymer, then respectively adding the water-insoluble polymer and the water-soluble polymer into N, N-dimethylformamide, heating and stirring until the water-insoluble polymer and the water-soluble polymer are completely dissolved, standing and cooling to room temperature, adding a hemostatic drug or an anti-inflammatory drug, stirring until the water-soluble polymer and the alcohol-soluble polymer are completely dissolved, and then pouring the core tube fluid into a core tube fluid reservoir;

the manufacturing steps of the nanofiber felt comprise:

selecting a coaxial electrostatic spinning machine which is adaptive to preset output, communicating the sheath film fluid reservoir with an outer spray pipe infusion pump of a coaxial spray head on the coaxial electrostatic spinning machine, adjusting the flow rate of the sheath film fluid, simultaneously communicating the core pipe fluid reservoir with an inner spray pipe infusion pump of the coaxial spray head of the coaxial electrostatic spinning machine, adjusting the flow rate of the core pipe fluid, connecting the coaxial spray head of the coaxial electrostatic spinning machine with a positive electrode of a high-voltage power supply of the coaxial electrostatic spinning machine, connecting a receiving flat plate of the coaxial electrostatic spinning machine with a negative electrode of the high-voltage power supply of the coaxial electrostatic spinning machine, adjusting the distance between the receiving flat plate and a spray nozzle of the coaxial spray head, setting the working environment temperature and the environment relative humidity of the coaxial electrostatic spinning machine, and starting the coaxial electrostatic spinning machine to manufacture nano fibers and receiving the nano fiber mat formed by stacking and felting the nano fibers from the receiving flat plate;

the surface treatment step of the nanofiber felt comprises the following steps:

immersing the receiving flat plate loaded with the nanofiber felt into a container filled with an erosion liquid for erosion, taking out the receiving flat plate loaded with the erosion object after the erosion is finished, drying and forming a film, wherein the erosion object after the film is dried and formed is the antibacterial and anti-inflammatory dressing.

Further:

in the preparation step of the sheath fluid, the heating is water bath heating, and the water bath temperature is 40 ℃;

in the preparation step of the core tube fluid, the heating is also water bath heating, and the water bath temperature is 50 ℃;

in the manufacturing step of the nanofiber felt, the receiving flat plate is made of aluminum foil, the flow rate of sheath fluid is 0.2-0.3 mL/h, the flow rate of core tube fluid is 0.4-0.6 mL/h, the voltage of the high-voltage power supply is 10-11 kV, the distance between the receiving flat plate and a spinning nozzle of the coaxial nozzle is 15cm, the working environment temperature of the coaxial electrostatic spinning machine is 20+/-1 ℃, and the relative humidity of the working environment of the coaxial electrostatic spinning machine is 45+/-5%.

Further:

the corrosion solution is absolute ethyl alcohol, the duration of corrosion is 15-30 minutes, and the drying temperature of the corrosion solution is 37 ℃.

Finally, the invention also provides an application of the antibacterial and anti-inflammatory dressing, namely:

cutting the antibacterial and anti-inflammatory dressing into small blocks and pasting the small blocks on an adhesive tape to form a wound plaster for sterilizing and anti-inflammatory nursing or sterilizing, anti-inflammatory and hemostatic nursing of small wounds on the surface of a body; or the antibacterial and anti-inflammatory dressing is cut and folded into a lamellar medicine felt for sterilizing and anti-inflammatory nursing or sterilizing, anti-inflammatory and hemostatic nursing of the wound of the organism.

Compared with the prior art, the invention has the beneficial effects and remarkable progress that:

firstly, the antibacterial and anti-inflammatory dressing provided by the invention is formed by stacking and felting nano fibers, wherein the nano fibers comprise a core tube and a sheath film coated outside the core tube, and the sheath film has a lace net-shaped porous structure, so that the structure and the function of natural skin can be well simulated and the natural skin has certain hydrophilicity, the nursing and healing requirements of a wound complex environment can be better met, and the good mechanical property of the wound complex environment can be maintained while a higher specific surface area is created;

in addition, the antibacterial and anti-inflammatory dressing provided by the invention has the advantages that the sheath film contains the nano metal antibacterial agent, and the core tube contains the hemostatic drug or the anti-inflammatory drug, so that the dressing not only has antibacterial efficacy, but also has hemostatic or anti-inflammatory effects, and can realize various applications, especially the reticular porous sheath film coated outside the core tube can effectively control the release of the hemostatic drug or the anti-inflammatory drug contained in the core tube, thereby effectively controlling the occurrence of toxic and side effects of the drug and obtaining better wound care efficacy;

meanwhile, the preparation of the nanofiber is carried out by adopting a relatively mature coaxial electrostatic spinning technology, so that the requirements of batch production such as simple preparation method, low production cost and the like can be met, and the nanofiber has a relatively good application prospect and a relatively high use value;

the sheath film forms a 'lace' net-shaped porous structure by an erosion method, so that the method is novel and creative, and can effectively control the formation and development of the 'lace' net-shaped porous structure by adjusting the erosion agent and the erosion time, thereby realizing the controlled release of hemostatic or anti-inflammatory drugs in the core tube, leading the hemostatic or anti-inflammatory drugs to exert better curative effect, reducing the harm of toxic and side effects and promoting the effective healing of wound parts.

Drawings

In order to more clearly describe the technical solution of the present invention and to implement the technical effects of the present invention, a brief description will be given below of the drawings used in the embodiments of the present invention.

Obviously:

the drawings described below are only illustrative of some of the embodiments of the present invention and other drawings may be made by those skilled in the art without the benefit of the inventive faculty, and are intended to fall within the scope of the invention as defined by the claims.

Fig. 1 is a schematic structural diagram of a nanofiber of an antibacterial and anti-inflammatory dressing according to an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a coaxial nozzle in a coaxial electrostatic spinning machine according to an embodiment of the present invention;

FIG. 3 is an electron microscope scan of the nanofibers of the anti-inflammatory dressing of example 9 of the present invention;

FIG. 4 is a graph showing the release profile of an anti-inflammatory dressing comprising a drug according to example 9 of the present invention;

FIG. 5 is a photograph of a circle of inhibition of Staphylococcus aureus with the anti-inflammatory dressing of example 9 of the present invention;

fig. 6 is a photograph of a circle of inhibition of escherichia coli with the anti-inflammatory dressing of example 9 of the present invention.

In the figure:

100-nanofibers, 110-core tubes, 120-sheath films;

200-coaxial spray heads, 210-inner spray pipes and 220-outer spray pipes.

Detailed Description

In order to make the objects, technical solutions, advantageous effects and significant improvements of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings provided by the embodiments of the present invention, and it is apparent that all of the described embodiments are only some embodiments of the present invention, not all embodiments.

All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to fall within the scope of the invention as claimed.

It should be noted that:

the terms first, second, and the like in the description and in the claims of the present invention and in the drawings of embodiments of the present invention, are used for distinguishing between different objects and not for describing a particular sequential manner;

furthermore, the terms "comprises," "comprising," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements but may, optionally, include other steps or elements not listed or inherent to such process, method, article, or apparatus.

It is to be understood that:

in the description of the embodiments of the present invention, some basic operation terms commonly used in the art, for example, "heating," "stirring," "mixing," "dissolving," "purifying," "filtering," and "drying," etc., are used, and it should be understood that these terms are not limited to the conventional operations performed by various conventional apparatuses and instruments in the art, but may also be program-controlled operations, unmanned automatic operations, etc. performed by the latest apparatuses, and unless otherwise specifically defined, those skilled in the art will understand the specific meaning of the terms in the present invention according to the specific circumstances and use specific operation methods to achieve the purpose of the operation.

Also to be described is:

the following embodiments may be combined with each other, and the same or similar concepts or processes may not be described in detail in some embodiments;

further, the raw materials, auxiliary materials, reaction equipment, facilities and the like, which are referred to in the following specific examples, are commercially available.

The following describes the technical scheme of the present invention in detail by using specific examples.

Example 1

The embodiment provides an antibacterial and anti-inflammatory dressing.

As shown in a schematic structural diagram of a nanofiber of an antibacterial and anti-inflammatory dressing provided in the embodiment of the invention in fig. 1:

an antibacterial and anti-inflammatory dressing is formed by stacking and felting nanofibers, wherein the nanofibers 100 comprise a core tube 110 and a sheath film 120 coated outside the core tube 110, the sheath film 120 has a lace net-shaped porous structure, the sheath film 120 contains nano metal antibacterial agents, and the core tube 110 contains hemostatic drugs or anti-inflammatory drugs.

From the above description, it can be seen that:

the antibacterial and anti-inflammatory dressing provided by the embodiment is formed by stacking and felting nano fibers, wherein the nano fibers comprise a core tube and a sheath film coated outside the core tube, the sheath film has a lace net-shaped porous structure, and the structure and the function of natural skin can be well simulated and the natural skin has certain hydrophilicity, so that the nursing and healing requirements of a wound complex environment can be well met, and the nano fibers of the antibacterial and anti-inflammatory dressing provided by the embodiment consist of the core tube and the sheath film coated outside the core tube, so that the antibacterial and anti-inflammatory dressing has a very large specific surface area and can still maintain good mechanical properties;

in addition, as the antibacterial and anti-inflammatory dressing provided by the embodiment contains the nano metal antibacterial agent in the sheath film and the hemostatic or anti-inflammatory drug in the core tube, the dressing not only has good and continuous antibacterial efficacy, but also has hemostatic or anti-inflammatory effects, thereby being capable of realizing various applications such as being used as a band-aid or wound dressing; especially, the reticular porous sheath membrane coated outside the core tube can effectively control the release of hemostatic or anti-inflammatory drugs contained in the core tube, effectively prolong the curative effect of the drugs and effectively control the occurrence of toxic and side effects of the drugs, thereby achieving better wound care efficacy.

Further:

the antibacterial and anti-inflammatory dressing provided in this embodiment has a core tube 110 and a sheath 120 in the nanofiber respectively formed by combining a water-insoluble polymer with a water-soluble polymer and an alcohol-soluble polymer.

In this example, the water-insoluble polymer is polyacrylonitrile, and the water-soluble and alcohol-soluble polymer is polyethylene glycol or polyvinylpyrrolidone.

Further, in this embodiment, the weight ratio of the water-insoluble polymer to the water-soluble and alcohol-soluble polymer is 0.6:1 or 1:0.6.

In the embodiment, the hemostatic drug is tranexamic acid, the anti-inflammatory drug is gentamicin sulfate, tetracycline hydrochloride, ibuprofen or ciprofloxacin, and the nano metal antibacterial agent is silver ion antibacterial agent.

Of course, the person skilled in the art can also select other commonly used water-insoluble polymers and water-soluble and alcohol-soluble polymers as the constituent materials of the core tube and the sheath according to the need, and can select other hemostatic drugs and anti-inflammatory drugs and nano-metal antibacterial agents which are proved to be suitable by experiments as the internal drugs and nano-metal antibacterial agents of the core tube and the sheath according to the need, so as to obtain other antibacterial and anti-inflammatory dressings, but the products obtained by the methods and the steps provided by the embodiments of the invention still belong to the scope of the protection claimed by the patent.

Example 2

The embodiment provides a preparation method of the antibacterial and anti-inflammatory dressing in embodiment 1, which specifically comprises the following steps:

a preparation step of sheath fluid, a preparation step of core tube fluid, a preparation step of nanofiber felt and a surface treatment step of nanofiber felt, wherein:

the preparation steps of the sheath fluid comprise:

respectively measuring or weighing nano metal antibacterial agent, water-insoluble polymer and water-soluble and alcohol-soluble polymer, then respectively adding into N, N-dimethylformamide, heating and stirring until the nano metal antibacterial agent, the water-insoluble polymer and the water-soluble and alcohol-soluble polymer are completely dissolved to obtain a sheath fluid, and then pouring the sheath fluid into a sheath fluid reservoir;

the preparation steps of the core tube fluid comprise:

measuring or weighing water-insoluble polymer and water-soluble and alcohol-soluble polymer respectively, adding into N, N-dimethylformamide respectively, heating and stirring until the water-insoluble polymer and the water-soluble and alcohol-soluble polymer are completely dissolved, standing and cooling to room temperature, adding hemostatic or anti-inflammatory drug and stirring until the water-soluble polymer and the alcohol-soluble polymer are completely dissolved to form core tube fluid, and pouring the core tube fluid into a core tube fluid reservoir;

the manufacturing steps of the nanofiber felt comprise:

selecting a coaxial electrostatic spinning machine adapting to preset output, communicating a sheath fluid reservoir with an outer spray pipe infusion pump of a coaxial spray head on the coaxial electrostatic spinning machine, adjusting the flow rate of the set sheath fluid, simultaneously communicating a core pipe fluid reservoir with an inner spray pipe infusion pump of the coaxial spray head of the coaxial electrostatic spinning machine, adjusting the flow rate of the set core pipe fluid, connecting the coaxial spray head of the coaxial electrostatic spinning machine with a positive electrode of a high-voltage power supply of the coaxial electrostatic spinning machine, connecting a receiving flat plate of the coaxial electrostatic spinning machine with a negative electrode of the high-voltage power supply of the coaxial electrostatic spinning machine, adjusting the distance between the receiving flat plate and a spray nozzle of the coaxial electrostatic spinning machine, setting the working ambient temperature and the ambient relative humidity of the coaxial electrostatic spinning machine, and starting the coaxial electrostatic spinning machine to manufacture nano fibers and receiving the nano fiber felt formed by stacking and felting the nano fibers from the receiving flat plate;

the surface treatment steps of the nanofiber felt comprise:

immersing the receiving flat plate loaded with the nanofiber felt into a container filled with an erosion liquid for erosion, taking out the receiving flat plate loaded with the erosion matter after the erosion is finished, and drying the receiving flat plate to form a film, wherein the erosion matter after the film is dried to obtain the antibacterial and anti-inflammatory dressing.

The preparation method comprises the following steps:

the heating in the steps of preparing the sheath fluid and the core tube fluid is water bath heating respectively, the water bath temperature during the preparation of the sheath fluid is 40 ℃, and the water bath temperature during the preparation of the core tube fluid is 50 ℃;

in the nanofiber felt manufacturing step, a receiving flat plate is made of aluminum foil, the flow rate of sheath fluid is 0.2-0.3 mL/h, the flow rate of core tube fluid is 0.4-0.6 mL/h, the voltage of a high-voltage power supply is 10-11 kV, the distance between the receiving flat plate and a spinning nozzle of a coaxial nozzle is 15cm, the working environment temperature of the coaxial electrostatic spinning machine is 20+/-1 ℃, and the relative humidity of the working environment of the coaxial electrostatic spinning machine is 45+/-5%; in the step of nanofiber felt surface treatment, the corrosion solution is absolute ethyl alcohol, the corrosion duration is 15-30 minutes, and the drying temperature is 37 ℃.

In order to further help understand the technical solution provided in the present embodiment, the specific operation process and the obtainable effects of the present embodiment, the preparation method and the effects provided in the present embodiment are further described below by specific examples.

Example 1

Preparation of nanofiber felt containing gentamicin sulfate drug

A selected coaxial electrostatic spinning machine was placed in a working environment with an ambient temperature of 20±1 ℃ and a relative humidity of 45±5%, wherein:

the coaxial electrostatic spinning machine has a coaxial nozzle 200 as shown in the schematic structural diagram of the coaxial nozzle in the coaxial electrostatic spinning machine provided in the embodiment of fig. 2, and the coaxial nozzle 200 has an inner nozzle 210 and an outer nozzle 220 sleeved outside the inner nozzle 210 and coaxial with the inner nozzle 210.

Then, respectively weighing or weighing 0.4mL of silver ion antibacterial agent with the trade name of Xian-Bai-dun, 0.6g of polyacrylonitrile and 1g of polyethylene glycol, then respectively adding 10mL of N, N-Dimethylformamide (English name: N, N-Dimethylformamide, english name: DMF), heating and stirring in a water bath at 40 ℃ until the mixture is completely dissolved, preparing a sheath fluid, then pouring the sheath fluid into a sheath fluid reservoir, communicating the sheath fluid reservoir with an external jet tube infusion pump of a coaxial nozzle of a coaxial electrostatic spinning machine, and setting the flow rate of the sheath fluid to be 0.2mL/h;

respectively weighing or weighing 1.4g of polyacrylonitrile and 0.2g of polyethylene glycol, adding into 10mL of DMF, heating and stirring in a water bath at 50 ℃ until the mixture is completely dissolved, standing and cooling to room temperature, adding 0.1g of gentamicin sulfate to prepare core tube fluid, pouring the core tube fluid into a core tube fluid reservoir, communicating the core tube fluid reservoir with an inner spray tube infusion pump of a coaxial nozzle of a coaxial electrostatic spinning machine, and setting the flow rate of the core tube fluid to be 0.6mL/h;

and finally, connecting a coaxial spray head of the coaxial electrostatic spinning machine with a positive electrode of a 10kV high-voltage power supply of the coaxial electrostatic spinning machine, connecting an aluminum foil receiving flat plate of the coaxial electrostatic spinning machine with a negative electrode of a high-voltage power supply of the coaxial electrostatic spinning machine, adjusting the distance between the receiving flat plate and a spinning nozzle of the coaxial spray head to be 15cm, and starting the coaxial electrostatic spinning machine to receive the nanofiber felt containing gentamicin sulfate medicine, which is formed by spraying the sheath fluid and the core tube fluid through the coaxial spray head on the receiving flat plate and then stacking felting on the receiving flat plate, from the receiving flat plate made of the aluminum foil.

Examples 2 to 7

Preparation of nanofiber felt after changing composition, proportion and preparation conditions of sheath membrane and core tube fluid

As shown in the data of table 1, the same method and procedure as in example 1 were adopted, and only by adjusting the composition and ratio of the sheath fluid and the core tube fluid, and the voltage of the high voltage power supply of the coaxial electrostatic spinning machine, different nanofiber mats including gentamycin sulfate in examples 2 to 3, tetracycline hydrochloride in examples 4 to 5, ibuprofen in example 6, and tranexamic acid in example 7 as shown in table 1 were obtained.

The composition, proportions, and partially modified preparation conditions of the sheath fluid and the core tube fluid in detail in examples 1 to 7 are shown in the following table 1.

TABLE 1

Example 8

Preparation of first antibacterial anti-inflammatory dressing containing tetracycline hydrochloride

Immersing the aluminum foil receiving plate containing the nano-fiber of the tetracycline hydrochloride prepared in the example 5 into a container filled with absolute ethyl alcohol serving as an etching solution for 15 minutes, taking out the aluminum foil receiving plate, placing the aluminum foil receiving plate in a drying oven with the drying temperature controlled at 37 ℃ for drying and film forming, and taking off the dried matter on the receiving plate after the drying and film forming is finished, thus obtaining the first antibacterial and anti-inflammatory dressing containing the tetracycline hydrochloride.

Example 9

Preparation of a second antibacterial anti-inflammatory dressing comprising tetracycline hydrochloride

The nanofiber containing tetracycline hydrochloride prepared in the above example 5 was also selected, and the operation was performed according to the same method and procedure as in example 8, and only the soaking time was changed from 15 minutes to 30 minutes, so as to obtain a second antibacterial and anti-inflammatory dressing containing tetracycline hydrochloride.

It should be noted that:

experiments have demonstrated that substantially the same technical effects as in example 8 or example 9 can be obtained by adjusting absolute ethanol as an etching solution to purified water and appropriately adjusting the etching time at the same time.

Effect example 1

Appearance characterization analysis of the antibacterial and anti-inflammatory dressing provided in this example

The second antibacterial and anti-inflammatory dressing containing tetracycline hydrochloride prepared in the above example 9 was subjected to surface metal spraying observation by using a Field Emission Scanning Electron Microscope (FESEM), so as to obtain an electron microscope scanning image of the nanofibers of the antibacterial and anti-inflammatory dressing provided in the example 9 shown in fig. 3.

As can be clearly seen from fig. 3:

the second anti-inflammatory and antibacterial dressing comprising tetracycline hydrochloride prepared in example 9 had a "lace" mesh-like porous structure on its surface.

Effect example 2

The antibacterial and anti-inflammatory dressing provided in this example has the analysis of drug release performance

Taking 100mg of the second antibacterial anti-inflammatory dressing containing tetracycline hydrochloride prepared in example 9, placing the second antibacterial anti-inflammatory dressing in a beaker filled with 450mL of phosphate buffer solution (PBS, pH=7.0) as a sample to be tested, placing the beaker in a water bath constant temperature oscillator of an RCZ-8A dissolution instrument according to a drug release detection method II (paddle method) specified in Chinese pharmacopoeia, setting the water bath temperature to 37 ℃ and the rotating speed to 50r/min, then at a preset time point, extracting 4.0mL of an aliquot sample, adding 4.0mL of freshly preheated PBS into the beaker to keep the total volume of the solution constant, and measuring each aliquot sample at lambda by using an ultraviolet-visible spectrophotometer _max The absorbance at 350nm was calculated to give the cumulative percent release of drug contained in the anti-inflammatory and antibacterial dressing according to example 9 of the present invention, and plotted as shown in fig. 4The present example 9 provides an antimicrobial anti-inflammatory dressing comprising a release profile of a drug.

As can be seen from fig. 4:

the anti-inflammatory dressing according to example 9 of the present invention contained a controlled release of the drug in a controlled release state without "explosion" type concentrated release.

Effect example 3

Antibacterial performance analysis is performed on the antibacterial and anti-inflammatory dressing provided by the embodiment through a bacteriostasis circle method

Cutting the antibacterial and anti-inflammatory dressing provided in example 9 of the present invention into dressing discs with a diameter of 6mm using a circular punch;

150. Mu.L of a suspension of gram-positive staphylococcus aureus (6538) and gram-negative escherichia coli (25922) with a concentration of 1X 105CFU/mL are added to the surfaces of the two agar plates respectively;

1 piece of the dressing disc is attached to the surface of each flat plate by using sterile forceps for inoculation, the dressing disc is fully contacted with agar by light pressure, the inoculated agar flat plates are placed into an incubator for 24 hours for culture, and then the diameter of a bacteriostasis ring is measured, wherein the internal temperature of the incubator is controlled at 37 ℃.

Each set of experiments is repeated three times, so that a photo image of a bacteriostasis area of the antibacterial anti-inflammatory dressing provided by the example 9 of the invention on staphylococcus aureus shown in fig. 5 and a photo image of a bacteriostasis area of the antibacterial anti-inflammatory dressing provided by the example 9 of the invention on escherichia coli shown in fig. 6 can be obtained.

From the photo image shown in fig. 5, it can be clearly seen that:

the antibacterial and anti-inflammatory dressing provided in example 9 of the invention has obvious antibacterial effects on staphylococcus aureus and escherichia coli.

From the above description, it can be seen that:

the antibacterial and anti-inflammatory dressing provided by the embodiment is a product formed by stacking and felting nanofibers, wherein the nanofibers are composed of a core tube and a sheath film coated outside the core tube, the sheath film is of a lace-shaped porous structure and contains nano metal antibacterial agents, and the core tube contains hemostatic drugs or anti-inflammatory drugs, so that the antibacterial and anti-inflammatory dressing has antibacterial efficacy and hemostatic or anti-inflammatory effects, and release of the hemostatic drugs or the anti-inflammatory drugs can be effectively controlled by the sheath film, so that toxic and side effects of the drugs can be effectively controlled, and better wound care efficacy can be obtained;

meanwhile, the preparation method provided by the embodiment adopts a mature coaxial electrostatic spinning technology, and can meet the requirements of batch and commercial production, so that the method has a good application prospect and a high use value;

the sheath film forms a 'lace' net-shaped porous structure by an erosion method, so that the method is novel and creative, and can effectively control the formation and development of the 'lace' net-shaped porous structure by adjusting the erosion agent and the erosion time, thereby realizing the controlled release of the blood stopping medicine or the anti-inflammatory medicine on the core tube, leading the core tube to exert better curative effect, reducing toxic and side effects and promoting the effective healing of wound parts.

What needs to be specifically stated is:

although the examples provided in this example are merely examples of the preparation methods of the partial products and the effect tests of the partial products of the present invention, it is sufficient for those skilled in the art that the preparation methods of the partial products and the effect tests of the partial products can be used to demonstrate that the preparation methods provided in this example can fulfill the preparation needs of all the products of the present invention; and it can be known from the knowledge level and common knowledge of the person skilled in the art that all the products according to the present invention can obtain the technical effects similar to those described in the effect examples 1 to 3; in addition, according to the characteristics of the constituent materials of the core tube and the sheath film, the person skilled in the art can know that all the products related to the invention have certain hydrophilicity; for brevity of the present specification, no further description is given here, and examples 1 to 7 and effect examples 1 to 3 are taken as general descriptions of the preparation method and the technical effect provided in this embodiment, so as to indicate that the preparation method and the technical effect of the antibacterial and anti-inflammatory dressing provided in the present invention have universality.

Example 3

The embodiment provides an application of an antibacterial and anti-inflammatory dressing.

The application of the antibacterial and anti-inflammatory dressing provided by the embodiment comprises the following steps:

the antibacterial and anti-inflammatory dressing prepared in the embodiment 2 is cut into small pieces and stuck on an adhesive tape to form a wound plaster for sterilizing and anti-inflammatory nursing or sterilizing, anti-inflammatory and hemostatic nursing of small wounds on the surface of a body; or (b)

The antibacterial and anti-inflammatory dressing prepared by the above example 2 was cut and folded into a sheet-like medicated felt for sterilizing and anti-inflammatory care or sterilizing and anti-inflammatory hemostatic care of a wound of a body.

From the above description, it can be seen that:

the application provided by the embodiment can comprise various forms and is convenient to apply, so that better wound care can be provided for wounded, the anti-inflammation healing of wounds is promoted, and the production requirements of batch and commercialization can be met, so that the wound dressing has a better application prospect and a higher development value.

From the above, it can be seen that:

the antibacterial and anti-inflammatory dressing provided by the invention is a nanofiber stacked felted product consisting of a core tube and an outer sheath film, wherein the sheath film is provided with a lace-shaped porous structure and contains a nano metal antibacterial agent, and the core tube contains a hemostatic drug or an anti-inflammatory drug, so that the antibacterial and anti-inflammatory dressing not only has antibacterial efficacy, but also has hemostatic or anti-inflammatory effects, and the release of the drug in the core tube can be effectively controlled through the sheath film, the toxic and side effects of the drug can be effectively controlled, and better nursing efficacy can be obtained;

meanwhile, the preparation method provided by the invention not only comprises the existing mature technology, but also has a unique innovative technology, can be applied in various ways, can meet the production requirements of batch and commercialization, has a better application prospect and higher development value, and has remarkable progress and excellent beneficial effects compared with the prior art.

In the description of the above specification:

the terms "present embodiment," "present example," "present effect example," "as … …," "further," and the like, describe herein means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention;

in this specification, a schematic representation of the above terms is not necessarily directed to the same embodiment or example, and the particular features, structures, materials, or characteristics described, etc. may be combined or combined in any suitable manner in any one or more embodiments or examples;

furthermore, various embodiments or examples, as well as features of various embodiments or examples, described in this specification may be combined or combined by one of ordinary skill in the art without undue experimentation.

Finally, it should be noted that:

while the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art that the foregoing embodiments may be modified or equivalents may be substituted for some or all of the features thereof, and that the modifications or substitutions may be made without departing from the spirit and scope of the embodiments of the present invention.

Claims (10)

1. An antibacterial and anti-inflammatory dressing, which is characterized in that:

the nano-fiber composite material is formed by stacking and felting nano-fibers, wherein the nano-fibers comprise a core tube and a sheath film coated outside the core tube, the sheath film is provided with a lace net-shaped porous structure, the sheath film contains nano-metal antibacterial agents, and the core tube contains hemostatic drugs or anti-inflammatory drugs.

2. The antimicrobial anti-inflammatory dressing of claim 1, wherein: the core tube and the sheath film are respectively formed by combining a water-insoluble polymer and water-soluble and alcohol-soluble polymers.

3. The antimicrobial anti-inflammatory dressing of claim 2, wherein: the water-insoluble polymer is polyacrylonitrile, and the water-soluble and alcohol-soluble polymer is polyethylene glycol or polyvinylpyrrolidone.

4. The antimicrobial anti-inflammatory dressing of claim 2, wherein: the weight ratio of the water-insoluble polymer to the water-soluble and alcohol-soluble polymer is 0.6:1 or 1:0.6.

5. The antimicrobial anti-inflammatory dressing of claim 2, wherein: the hemostatic drug is tranexamic acid, and the anti-inflammatory drug is any one of gentamicin sulfate, tetracycline hydrochloride, ibuprofen or ciprofloxacin.

6. The antimicrobial anti-inflammatory dressing of claim 2, wherein: the nano metal antibacterial agent is a silver ion antibacterial agent.

7. A method of preparing an anti-inflammatory dressing according to any one of claims 1 to 6, comprising the steps of:

a preparation step of sheath fluid, a preparation step of core tube fluid, a preparation step of nanofiber felt and a surface treatment step of nanofiber felt, wherein:

the preparation steps of the sheath fluid comprise:

respectively measuring or weighing nano metal antibacterial agent, water-insoluble polymer and water-soluble and alcohol-soluble polymer, then respectively adding into N, N-dimethylformamide, heating and stirring until the nano metal antibacterial agent, the water-insoluble polymer and the water-soluble and alcohol-soluble polymer are completely dissolved to obtain a sheath fluid, and then pouring the sheath fluid into a sheath fluid reservoir;

the preparation steps of the core tube fluid comprise:

respectively measuring or weighing a water-insoluble polymer and a water-soluble polymer and an alcohol-soluble polymer, then respectively adding the water-insoluble polymer and the water-soluble polymer into N, N-dimethylformamide, heating and stirring until the water-insoluble polymer and the water-soluble polymer are completely dissolved, standing and cooling to room temperature, adding a hemostatic drug or an anti-inflammatory drug, stirring until the water-soluble polymer and the alcohol-soluble polymer are completely dissolved, and then pouring the core tube fluid into a core tube fluid reservoir;

the manufacturing steps of the nanofiber felt comprise:

selecting a coaxial electrostatic spinning machine which is adaptive to preset output, communicating the sheath film fluid reservoir with an outer spray pipe infusion pump of a coaxial spray head on the coaxial electrostatic spinning machine, adjusting the flow rate of the sheath film fluid, simultaneously communicating the core pipe fluid reservoir with an inner spray pipe infusion pump of the coaxial spray head of the coaxial electrostatic spinning machine, adjusting the flow rate of the core pipe fluid, connecting the coaxial spray head of the coaxial electrostatic spinning machine with a positive electrode of a high-voltage power supply of the coaxial electrostatic spinning machine, connecting a receiving flat plate of the coaxial electrostatic spinning machine with a negative electrode of the high-voltage power supply of the coaxial electrostatic spinning machine, adjusting the distance between the receiving flat plate and a spray nozzle of the coaxial spray head, setting the working environment temperature and the environment relative humidity of the coaxial electrostatic spinning machine, and starting the coaxial electrostatic spinning machine to manufacture nano fibers and receiving the nano fiber mat formed by stacking and felting the nano fibers from the receiving flat plate;

the surface treatment step of the nanofiber felt comprises the following steps:

immersing the receiving flat plate loaded with the nanofiber felt into a container filled with an erosion liquid for erosion, taking out the receiving flat plate loaded with the erosion object after the erosion is finished, drying and forming a film, wherein the erosion object after the film is dried and formed is the antibacterial and anti-inflammatory dressing.

8. The method of manufacturing according to claim 7, wherein:

in the preparation step of the sheath fluid, the heating is water bath heating, and the water bath temperature is 40 ℃;

in the preparation step of the core tube fluid, the heating is also water bath heating, and the water bath temperature is 50 ℃;

in the manufacturing step of the nanofiber felt, the receiving flat plate is made of aluminum foil, the flow rate of sheath fluid is 0.2-0.3 mL/h, the flow rate of core tube fluid is 0.4-0.6 mL/h, the voltage of the high-voltage power supply is 10-11 kV, the distance between the receiving flat plate and a spinning nozzle of the coaxial nozzle is 15cm, the working environment temperature of the coaxial electrostatic spinning machine is 20+/-1 ℃, and the relative humidity of the working environment of the coaxial electrostatic spinning machine is 45+/-5%.

9. The method of manufacturing according to claim 7, wherein: the corrosion solution is absolute ethyl alcohol, the duration of corrosion is 15-30 minutes, and the drying temperature of the corrosion solution is 37 ℃.

10. Use of an anti-inflammatory and antibacterial dressing according to any one of claims 1 to 6, characterized in that:

cutting the antibacterial and anti-inflammatory dressing into small blocks and pasting the small blocks on an adhesive tape to form a wound plaster for sterilizing and anti-inflammatory nursing or sterilizing, anti-inflammatory and hemostatic nursing of small wounds on the surface of a body; or (b)

The antibacterial and anti-inflammatory dressing is cut and folded into a lamellar medicine felt for sterilizing and anti-inflammatory nursing or sterilizing, anti-inflammatory and hemostatic nursing of the wound of the organism.