CN117801954B - Bionic culture device for embryonic stem cells - Google Patents
Bionic culture device for embryonic stem cells Download PDFInfo
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- CN117801954B CN117801954B CN202410024126.6A CN202410024126A CN117801954B CN 117801954 B CN117801954 B CN 117801954B CN 202410024126 A CN202410024126 A CN 202410024126A CN 117801954 B CN117801954 B CN 117801954B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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Abstract
本发明公开的一种胚胎干细胞仿生培养装置，涉及细胞培养技术领域，包括安装在壳体内的离心机构和安装在离心机构上的进液机构，所述离心机构包括安全组件、支座，支座上转动安装有转轴，转轴上套设有保护弹簧，保护弹簧的第一端与转轴连接，保护弹簧的第二端与压紧圈连接，压紧圈滑动安装在转轴上，转轴与安装盘转动连接，离心平台插装在安装盘上，所述安全组件包括与安装盘转动连接的固定圈，固定圈与防护罩连接；本发明通过设计离心机构和进液机构，对冻存的胚胎干细胞滴加入胚胎干细胞培养基，并进行离心，实验效率高。
The invention discloses an embryonic stem cell bionic culture device, which relates to the technical field of cell culture, and comprises a centrifugal mechanism installed in a shell and a liquid inlet mechanism installed on the centrifugal mechanism. The centrifugal mechanism comprises a safety component and a support. A rotating shaft is rotatably installed on the support. A protection spring is sleeved on the rotating shaft. The first end of the protection spring is connected to the rotating shaft, and the second end of the protection spring is connected to a clamping ring. The clamping ring is slidably installed on the rotating shaft. The rotating shaft is rotatably connected to a mounting plate. A centrifugal platform is inserted on the mounting plate. The safety component comprises a fixing ring rotatably connected to the mounting plate, and the fixing ring is connected to a protective cover. The invention designs the centrifugal mechanism and the liquid inlet mechanism, drips embryonic stem cell culture medium into frozen embryonic stem cells, and centrifuges the cells, so that the experimental efficiency is high.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a bionic culture device for embryonic stem cells.
Background
Embryonic stem cells are present in early embryos and have the potential to differentiate into any type of cell in the adult animal body and further form all tissues and organs of the body and even individuals. The basic process of the subculture of the human embryonic stem cells can be divided into reagent preparation, determination of the passage time, collagenase treatment, collection and breaking of clones, centrifugal passage, cryopreservation and resuscitation. In resuscitating, the frozen embryonic stem cells are removed from the liquid nitrogen, immersed in a water bath, and the frozen tube is then removed from the water bath and sterilized and air-dried. Transferring cells from a freezing tube into a centrifuge tube, dropwise adding an embryonic stem cell culture medium, and centrifuging, wherein the flow has low automation degree and low experimental efficiency.
The Chinese patent publication No. CN108018209A provides a device for culturing cells and strains, which comprises a matrix A, a containing cavity A, a solenoid, a channel pipe, a control valve and a first connecting pipe, wherein the device adopts a water bath method, the bionic culture device for the embryonic stem cells has a simple structure, can provide a constant temperature environment, is beneficial to cell and strain culture, but cannot solve the technical problem which can be solved by the bionic culture device for the embryonic stem cells.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a bionic culture device for embryonic stem cells, which overcomes the problems in the prior art.
The technical scheme includes that the embryonic stem cell bionic culture device comprises a centrifugal mechanism and a liquid inlet mechanism, wherein the centrifugal mechanism is arranged in a shell, the liquid inlet mechanism is arranged on the centrifugal mechanism, the centrifugal mechanism comprises a safety component and a support, a rotating shaft is rotatably arranged on the support, a protection spring is sleeved on the rotating shaft, a first end of the protection spring is connected with the rotating shaft, a second end of the protection spring is connected with a pressing ring, the pressing ring is slidably arranged on the rotating shaft, the rotating shaft is rotatably connected with a mounting disc, a centrifugal platform is inserted on the mounting disc, the safety component comprises a fixed ring rotatably connected with the mounting disc, the fixed ring is connected with a protective cover, an inserting block is slidably arranged on the fixed ring, the inserting block is matched with a groove on the mounting disc, a plurality of inserting blocks are uniformly distributed, each inserting block is rotatably connected with a sliding column, the sliding column is slidably arranged on a safety rotating ring, the safety rotating ring rotates around an axis, and the safety rotating ring is rotatably connected with the fixed ring.
Further, the safety swivel is fixedly connected with the bull gear coaxially, the bull gear is meshed with the safety gear, the safety gear is fixedly connected with the knob coaxially, and the knob is rotatably arranged on the shell.
Further, install feed liquor mechanism on the protection casing, feed liquor mechanism includes the cooperation section of thick bamboo of fixed mounting on the protection casing, is provided with the slider on the cooperation section of thick bamboo, and slider slidable mounting on the cooperation section of thick bamboo is in the helicla flute of hob, and the cover is equipped with reset spring on the hob, and reset spring's first end is connected with the hob, and reset spring's second end is connected with the cooperation section of thick bamboo.
Further, a lower guide disc is fixedly arranged on the protective cover, a plurality of sliding sheets are uniformly and slidably arranged on the lower guide disc, each sliding sheet is respectively connected with a sliding rod, the sliding rods are slidably arranged on the upper guide disc, and the upper guide disc is fixedly connected with the rotating frame.
Further, a blanking port is arranged on the lower guide disc, and adjacent sliding sheets are stuck together in a normal state and the blanking port is blocked.
Further, the rotating frame is rotatably arranged in the protective cover, the rotating frame is in sliding fit with the mounting rod, and the mounting rod is fixedly arranged on the screw rod.
Further, the first end of the screw rod is connected with the pressure rod, and the second end of the screw rod is connected with the drip tube.
Further, a viewing window is provided on the shield.
The centrifugal tube cleaning device has the advantages that (1) the spiral rod is in threaded fit with the sliding block on the matching cylinder by pressing the pressing rod to downwards press the spiral rod, the return spring is compressed, the spiral rod rotates when moving downwards to drive the installation rod to rotate, the installation rod drives the rotating frame to rotate to drive the upper guide disc to rotate, the sliding rod drives the sliding sheets to slide on the upper guide disc to enable the sliding sheets to be separated, blanking ports are exposed, the dropping tube drops embryonic stem cell culture medium into the centrifugal tube, the normally lower pressing ring is pressed on the installation disc under the elastic force of the protection spring to enable the installation disc to be in contact with the rotating shaft, the driving gear is driven to rotate by the starting motor to drive the driving gear to drive the large gear to rotate, the small gear ring drives the rotating shaft to rotate, the rotating shaft drives the pressing ring and the installation disc to rotate by the elastic force of the protection spring to drive the centrifugal platform to centrifuge, and (3) the centrifugal tube cleaning device is prevented from being suddenly affected by lifting the protection cover, taking out for subsequent operation, then taking out the centrifugal platform to clean, and limiting the positions of the installation disc and the centrifugal tube cleaning platform through the inserting blocks in the installation disc.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention.
FIG. 2 is a schematic view of a part of the centrifugal mechanism of the present invention.
Fig. 3 is a schematic diagram showing a partial structure of a centrifugal mechanism and a liquid inlet mechanism according to the present invention.
FIG. 4 is a schematic view of a partial cross-sectional structure of a centrifugal mechanism of the present invention.
Fig. 5 is a schematic view of a partial explosion structure of the centrifugal mechanism of the present invention.
Fig. 6 is a schematic diagram of a partial explosion structure of the centrifugal mechanism of the present invention.
Fig. 7 is a schematic diagram of a partial structure of a centrifugal mechanism and a liquid inlet mechanism according to the present invention.
Fig. 8 is a schematic diagram of a partial structure of a liquid feeding mechanism according to the present invention.
Fig. 9 is a schematic diagram of a partial structure of a liquid feeding mechanism according to the present invention.
FIG. 10 is a schematic view of a partial cross-sectional structure of a liquid feeding mechanism according to the present invention.
The reference numerals comprise a 1-centrifugal mechanism, a 2-liquid inlet mechanism, a 101-motor, a 102-support, a 103-driving gear, a 104-large gear, a 105-knob, a 106-small gear ring, a 107-rotating shaft, a 108-protection spring, a 109-pressing ring, a 110-safety rotating ring, a 111-large gear ring, a 112-sliding column, a 113-inserting block, a 114-mounting plate, a 115-fixing ring, a 116-centrifugal platform, a 117-protective cover, a 118-safety gear, a 201-pressing rod, a 202-reset spring, a 203-spiral rod, a 204-matching cylinder, a 205-mounting rod, a 206-rotating frame, a 207-upper guide plate, a 208-sliding rod, a 209-lower guide plate, a 210-sliding sheet and a 211-dropping liquid pipe.
Description of the embodiments
The invention will be described in further detail with reference to the accompanying drawings and specific preferred embodiments.
1-10, The embryonic stem cell bionic culture device comprises a centrifugal mechanism 1 and a liquid inlet mechanism 2, wherein the centrifugal mechanism 1 is arranged in a shell, the liquid inlet mechanism 2 is arranged on the centrifugal mechanism 1, the centrifugal mechanism 1 comprises a safety component, a motor 101, a support 102, a driving gear 103, a large gear 104, a small gear ring 106, a rotating shaft 107, a protection spring 108, a pressing ring 109, a mounting disc 114, a centrifugal platform 116 and a protective cover 117; the rotary shaft 107 is rotatably arranged on the support 102, the rotary shaft 107 is fixedly connected with the small gear ring 106, the small gear ring 106 is meshed with the large gear 104, the large gear 104 is rotatably arranged on the support 102, the large gear 104 is meshed with the driving gear 103, the driving gear 103 is connected with an output shaft of the motor 101, the motor 101 is arranged on the support 102, the support 102 is arranged on a shell, the rotary shaft 107 is sleeved with the protection spring 108, a first end of the protection spring 108 is connected with the rotary shaft 107, a second end of the protection spring 108 is connected with the pressing ring 109, the pressing ring 109 is slidably arranged on the rotary shaft 107, the protection spring 108 is in a compressed state, the rotary shaft 107 is rotatably connected with the mounting plate 114, the pressing ring 109 is contacted with the mounting plate 114, the centrifugal platform 116 is inserted on the mounting plate 114, the safety assembly comprises a knob 105, a safety rotating ring 110, the large gear ring 111, a sliding column 112, an inserting block 113, a fixed ring 115 and a safety gear 118, the fixed ring 115 is rotatably connected with the mounting plate 114, the fixed ring 115 is slidably arranged on the fixed ring 115, the inserting block 113 is matched with a groove on the mounting plate 114, the inserting block 113 is uniformly distributed on the fixed ring 113, and is rotatably connected with the rotating ring 110 through the sliding column 110 respectively, the sliding column 110 is rotatably connected with the fixed ring 110, the retainer 115 is fixedly mounted to the support 102.
The safety swivel 110 is fixedly connected with the bull gear 111 coaxially, the safety swivel 110 is rotatably mounted on the support 102, the bull gear 111 is meshed with the safety gear 118, the safety gear 118 is rotatably mounted on the support 102, the safety gear 118 is fixedly connected with the knob 105 coaxially, and the knob 105 is rotatably mounted on the housing.
The liquid inlet mechanism 2 is arranged on the protective cover 117, the liquid inlet mechanism 2 comprises a compression bar 201, a return spring 202, a spiral rod 203, a matching cylinder 204, a mounting bar 205, a rotating frame 206, an upper guide disc 207, a sliding bar 208, a lower guide disc 209, a sliding sheet 210 and a liquid dropping tube 211, the matching cylinder 204 is fixedly arranged on the protective cover 117, a sliding block is arranged on the matching cylinder 204, the sliding block on the matching cylinder 204 is slidably arranged in a spiral groove of the spiral rod 203, the spiral rod 203 is sleeved with the return spring 202, a first end of the return spring 202 is connected with the spiral rod 203, and a second end of the return spring 202 is connected with the matching cylinder 204.
The lower guide plate 209 is fixedly arranged on the protective cover 117, a plurality of sliding sheets 210 are uniformly and slidably arranged on the lower guide plate 209, each sliding sheet 210 is respectively connected with a sliding rod 208, the sliding rods 208 are slidably arranged on the upper guide plate 207, and the upper guide plate 207 is fixedly connected with the rotating frame 206.
The lower guide plate 209 is provided with a blanking port, and adjacent sliding sheets 210 are stuck together and block the blanking port in a normal state.
The rotating frame 206 is rotatably installed in the protecting cover 117, the rotating frame 206 is in sliding fit with the installation rod 205, and the installation rod 205 is fixedly arranged on the screw rod 203.
The first end of the screw 203 is connected to a plunger 201, the plunger 201 is slidably mounted on the housing, and the second end of the screw 203 is connected to a drip tube 211.
The boot 117 is provided with a viewing window.
The working principle of the invention is that the protection spring 108 is always kept in a compressed state, the centrifugal platform 116 is inserted on the mounting disc 114, a centrifugal tube which needs to be centrifuged is placed in the centrifugal platform 116, and the position of the centrifugal tube corresponds to the position of the drip tube 211. By pressing the pressing rod 201, pressing down the spiral rod 203, compressing the reset spring 202, rotating the spiral rod 203 in threaded fit with the sliding block on the matching barrel 204 when the spiral rod 203 moves down, driving the installation rod 205 to rotate, driving the rotating frame 206 to rotate, driving the upper guide plate 207 to rotate, driving the sliding rod 208 to slide on the upper guide plate 207, driving the sliding sheet 210 to slide on the lower guide plate 209, separating the sliding sheets 210, exposing the blanking port, dropping the embryonic stem cell culture medium into the centrifuge tube by the dropping tube 211, and resetting the spiral rod 203 under the elastic force of the reset spring 202 after dropping.
The pressing ring 109 is pressed on the mounting plate 114 under the elastic force of the protection spring 108 in a normal state, so that the mounting plate 114 is in contact with the rotating shaft 107, the driving gear 103 is driven by the starting motor 101 to rotate so as to drive the large gear 104 to rotate, the small gear ring 106 is driven by the small gear ring 106 to rotate so as to drive the rotating shaft 107 to rotate, the pressing ring 109 and the mounting plate 114 are driven by the elastic force of the protection spring 108 to rotate through the rotating shaft 107, and the centrifugal platform 116 is driven by the mounting plate 114 to rotate so as to perform centrifugation.
After centrifugation is finished, the knob 105 is manually twisted, the knob 105 drives the safety gear 118 to rotate, the bull gear 111 is driven to rotate, the safety swivel 110 is driven to rotate, the sliding column 112 is driven to slide on the safety swivel 110, the insert block 113 is pushed to slide on the fixed ring 115, the insert block 113 is driven to be clamped into a groove on the mounting disc 114, at the moment, the mounting disc 114 cannot rotate, and the position of the knob 105 is locked.
The boot 117 is lifted and the centrifuge tube is removed for subsequent operations. And then the centrifugal platform 116 is taken out for cleaning and disinfection, and the positions of the mounting plate 114 and the centrifugal platform 116 are limited by the insert blocks 113 clamped in the grooves of the mounting plate 114, so that the phenomenon that the device suddenly operates when the centrifugal tube is taken out or the device is cleaned later is avoided, and the experimental safety is influenced.
The centrifugal change in the boot 117 may be observed through a viewing window.
It should be understood that the foregoing embodiments are merely illustrative of the technical solutions of the present invention and not limiting thereof, and that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, and all such modifications and substitutions are intended to fall within the scope of the appended claims.
Claims (2)
1. A bionic culture device for embryonic stem cells comprises a centrifugal mechanism (1) arranged in a shell and a liquid inlet mechanism (2) arranged on the centrifugal mechanism (1), and is characterized in that the centrifugal mechanism (1) comprises a safety component and a support (102), a rotating shaft (107) is rotatably arranged on the support (102), a protection spring (108) is sleeved on the rotating shaft (107), a first end of the protection spring (108) is connected with the rotating shaft (107), a second end of the protection spring (108) is connected with a pressing ring (109), the pressing ring (109) is slidably arranged on the rotating shaft (107), the rotating shaft (107) is rotatably connected with a mounting disc (114), a centrifugal platform (116) is inserted on the mounting disc (114), the safety component comprises a fixed ring (115) rotatably connected with the mounting disc (114), the fixed ring (115) is connected with a protective cover (117), a plurality of inserting blocks (113) are slidably arranged on the fixed ring (115), the inserting blocks (113) are matched with grooves on the mounting disc (114), the inserting blocks (113) are uniformly distributed, each inserting block (113) is slidably connected with a rotating column (110) around the rotating shaft center (110) respectively, the safety swivel (110) is rotationally connected with the fixed ring (115);
The safety swivel (110) is fixedly connected with the bull gear (111) coaxially, the bull gear (111) is meshed with the safety gear (118), the safety gear (118) is fixedly connected with the knob (105) coaxially, and the knob (105) is rotatably arranged on the shell;
The protective cover (117) is provided with a liquid inlet mechanism (2), the liquid inlet mechanism (2) comprises a matching cylinder (204) fixedly arranged on the protective cover (117), a sliding block is arranged on the matching cylinder (204), the sliding block on the matching cylinder (204) is slidably arranged in a spiral groove of a spiral rod (203), the spiral rod (203) is sleeved with a reset spring (202), a first end of the reset spring (202) is connected with the spiral rod (203), and a second end of the reset spring (202) is connected with the matching cylinder (204);
a lower guide disc (209) is fixedly arranged on the protective cover (117), a plurality of sliding sheets (210) are uniformly and slidably arranged on the lower guide disc (209), each sliding sheet (210) is respectively connected with a sliding rod (208), the sliding rods (208) are slidably arranged on an upper guide disc (207), and the upper guide disc (207) is fixedly connected with the rotating frame (206);
a blanking port is arranged on the lower guide disc (209), and adjacent sliding sheets (210) are stuck together and block the blanking port in a normal state;
The rotating frame (206) is rotatably arranged in the protective cover (117), the rotating frame (206) is in sliding fit with the mounting rod (205), and the mounting rod (205) is fixedly arranged on the screw rod (203);
The first end of the screw rod (203) is connected with the compression rod (201), and the second end of the screw rod (203) is connected with the drip tube (211);
The protection spring (108) is always kept in a compressed state, the centrifugal platform (116) is inserted on the mounting disc (114), a centrifugal tube needing to be centrifuged is placed in the centrifugal platform (116), the position of the centrifugal tube corresponds to that of the liquid dropping tube (211), the spiral rod (203) is pressed down by pressing the pressing rod (201), the reset spring (202) is compressed, the spiral rod (203) is in threaded fit with the sliding block on the matching cylinder (204), the spiral rod (203) rotates when moving downwards, the mounting rod (205) is driven to rotate, the mounting rod (205) drives the rotating frame (206) to rotate, the upper guide disc (207) is driven to rotate, the sliding rod (208) is driven to slide on the upper guide disc (207), the sliding rod (210) is driven to slide on the lower guide disc (209), the sliding rods (210) are separated, the blanking port is exposed, the liquid dropping tube (211) drops embryonic stem cell culture medium into the liquid dropping tube, and after the dropping, the spiral rod (203) resets under the elastic force of the reset spring (202);
The pressing ring (109) is pressed on the mounting disc (114) under the elastic force of the protection spring (108) in a normal state, so that the mounting disc (114) is in contact with the rotating shaft (107), the starting motor (101) drives the driving gear (103) to rotate so as to drive the large gear (104) to rotate, the small gear ring (106) is driven to rotate, the small gear ring (106) drives the rotating shaft (107) to rotate, the rotating shaft (107) drives the pressing ring (109) and the mounting disc (114) to rotate through the elastic force of the protection spring (108), and the mounting disc (114) drives the centrifugal platform (116) to rotate for centrifugation;
after centrifugation is finished, the knob (105) is manually twisted, the knob (105) drives the safety gear (118) to rotate, the bull gear (111) is driven to rotate, the safety swivel (110) is driven to rotate, the sliding column (112) is driven to slide on the safety swivel (110), the insert block (113) is pushed to slide on the fixed ring (115), the insert block (113) is driven to be clamped into a groove on the mounting disc (114), at the moment, the mounting disc (114) cannot rotate, and the position of the knob (105) is locked;
lifting the protective cover (117), taking out the centrifuge tube for subsequent operation, then taking out the centrifugal platform (116) for cleaning and disinfection, and limiting the positions of the mounting plate (114) and the centrifugal platform (116) through the insertion blocks (113) clamped into the grooves of the mounting plate (114).
2. The bionic culture apparatus according to claim 1, wherein the protective cover (117) is provided with a viewing window.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410024126.6A CN117801954B (en) | 2024-01-08 | 2024-01-08 | Bionic culture device for embryonic stem cells |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410024126.6A CN117801954B (en) | 2024-01-08 | 2024-01-08 | Bionic culture device for embryonic stem cells |
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| CN117801954A CN117801954A (en) | 2024-04-02 |
| CN117801954B true CN117801954B (en) | 2024-12-24 |
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| CN202410024126.6A Active CN117801954B (en) | 2024-01-08 | 2024-01-08 | Bionic culture device for embryonic stem cells |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN118995420B (en) * | 2024-10-25 | 2024-12-27 | 深圳知因细胞生物科技有限公司 | Bionic culture device for embryonic stem cells |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108018209A (en) * | 2018-02-08 | 2018-05-11 | 绍兴市上虞区中泰医疗科技有限公司 | One kind culture cell and strain device |
| CN112779137A (en) * | 2021-01-27 | 2021-05-11 | 王墨权 | Stem cell culture method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7915034B2 (en) * | 2006-06-29 | 2011-03-29 | Genx International | Apparatus and method for culturing oocytes, embryos, stem cells and cells |
| CN220132240U (en) * | 2023-06-27 | 2023-12-05 | 细胞谷(南京)生物科技有限公司 | Separating device |
| CN117339772B (en) * | 2023-12-06 | 2024-03-05 | 江苏康为世纪生物科技股份有限公司 | Test tube centrifuge for reagent preparation |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108018209A (en) * | 2018-02-08 | 2018-05-11 | 绍兴市上虞区中泰医疗科技有限公司 | One kind culture cell and strain device |
| CN112779137A (en) * | 2021-01-27 | 2021-05-11 | 王墨权 | Stem cell culture method |
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