CN117731581A - Scalp care anti-hair loss composition and application thereof, hair shampoo product and preparation method thereof - Google Patents

Abstract

The present application relates to the field of cosmetics, specifically, to a scalp care and anti-hair loss composition and its application, a hair shampoo product and its preparation method. A scalp care and anti-hair loss composition. In terms of mass percentage, the scalp care and anti-hair loss composition includes: ginger juice 1.0% to 35%, allantoin 0.01% to 2.0%, and panthenol 0.1% to 10.0%. Cypress leaf extract 0.1% ~ 5%, hydrolyzed chitin 0.005% ~ 0.1%, salicylic acid 0.01% ~ 0.5%; the balance is water. The anti-hair loss composition for scalp care provided by this application has synergistic cooperation between each component to achieve a synergistic effect; the role of each component is maximized, from scalp hair follicles, scalp inflammation, multiple pathways, and multi-dimensional care Scalp, make the scalp more nutritious, make the scalp healthier, thereby achieving the best ideal state of anti-hair loss effect, and ultimately achieve the effect of anti-hair loss.

Description

Scalp care anti-hair loss composition and application thereof, hair shampoo product and preparation thereof method

Technical field

The present application relates to the field of cosmetics, specifically, to a scalp care and anti-hair loss composition and its application, a hair shampoo product and its preparation method.

Background technique

At present, due to the increasing pressure of life, scalp and hair loss problems are also increasing. There are many shampoo products on the market, but they are mainly used for cleaning and caring for hair. There are also more anti-hair loss shampoo products, but many of them are just Make the hair smoother and reduce the pulling force when combing, so that it looks like less hair falls out, but this is all a superficial phenomenon.

In order to prevent hair loss, surgical treatment or drug treatment can also be used, but the cost is high and the safety is poor. If it is stimulated by improper care, it may cause hair loss again.

Contents of the invention

The purpose of the embodiments of the present application is to provide a scalp care and anti-hair loss composition and its application, a hair shampoo product and a preparation method thereof.

The scalp is different from the skin on other parts of the body in that it has a large number of large hair follicles that produce thick and long hair fibers, and each hair follicle is attached to a large sebaceous gland. In addition, hair follicles are closely connected with intricate nerve and blood vessel networks. So there are many reasons for hair loss. First of all, pilosebaceous glands can store a considerable amount of sebum. In normal individuals, sebaceous glands show a complex secretion rhythm, and sebum-rich pores provide space for lipophilic microorganisms and parasites, such as Propionibacterium and Epidermis. An aerobic environment such as Staphylococcus aureus, Malassezia, and helminths and mites can cause scalp inflammation and hair loss. Secondly, if the blood vessel network connected to the hair follicles has blocked blood circulation, it is often said that the hair follicles are undernourished. It can cause hair loss; thirdly, when there is inflammation in the scalp, it can also cause hair loss.

In view of this, this application mainly uses shampoo products to provide daily care and repair of the scalp from various targets of the hair loss mechanism from multiple dimensions, so as to make the scalp healthier and achieve the purpose of preventing hair loss.

In the first aspect, this application provides a scalp care and anti-hair loss composition. In terms of mass percentage, the scalp care and anti-hair loss composition includes:

Ginger juice 1.0% ~ 35%, allantoin 0.01% ~ 2.0%, panthenol 0.1% ~ 10.0%, Platycladus orientalis leaf extract 0.1% ~ 5%, hydrolyzed chitin 0.005% ~ 0.1%, salicylic acid 0.01% ~0.5%; the balance is water.

The anti-hair loss composition for scalp care provided by this application has synergistic cooperation between each component to achieve a synergistic effect; the role of each component is maximized, from multiple pathways of scalp hair follicles and scalp inflammation to multi-dimensional care. Scalp, make the scalp more nutritious, make the scalp healthier, thereby achieving the best and ideal state of preventing hair loss, and ultimately achieve the effect of preventing hair loss.

Applying the scalp care and anti-hair loss composition of the present application to a hair shampoo product can make the hair care product achieve a hair care and anti-hair loss effect. Thus, it can replace conventional anti-hair loss chemotherapy drugs and medical treatments, embodying the concept of convenience, health and safety.

In other embodiments of the present application, the method of using the above-mentioned scalp care and anti-hair loss composition includes: directly compounding the scalp care and anti-hair loss composition into a hair shampoo product for daily shampooing; convenient, simple and fast .

In other embodiments of the present application, in terms of mass percentage, the anti-hair loss composition with scalp care includes:

Ginger juice 5% to 20%, allantoin 0.05% to 1.0%, panthenol 0.2% to 5%, Platycladus orientalis leaf extract 0.3% to 3%, hydrolyzed chitin 0.01% to 0.08%, salicylic acid 0.05% ~0.3%; the balance is water.

In other embodiments of the present application, in terms of mass percentage, the anti-hair loss composition with scalp care includes:

Ginger juice 12%, allantoin 0.3%, panthenol 1.0%, Platycladus orientalis leaf extract 0.9%, hydrolyzed chitin 0.016%, salicylic acid 0.1%, the balance is water.

In other embodiments of the present application, the active ingredients in ginger juice include: gingerols, parazingerones, zingiberones, and gingerdiones.

In other embodiments of the present application, the active ingredients in Platycladus orientalis leaf extract include: Cypress biflavonoids, Cryptomeria cypress biflavonoids, Cryptomeria cypress biflavonoids, quercetin, quercetin, myricetin, kaempferol phenol.

In other embodiments of the present application, the scalp care anti-hair loss composition does not contain purslane extract.

In a second aspect, this application provides the application of the scalp care and anti-hair loss composition provided in the first aspect in a hair shampoo product.

In a third aspect, the present application provides a hair shampoo product, including the scalp care and anti-hair loss composition provided in the first aspect.

In other embodiments of the present application, hair shampoo products include:

10 to 15 parts of a scalp care and anti-hair loss composition;

10 to 18 parts of ammonium laureth sulfate;

1 to 5 parts of ammonium lauryl sulfate;

1 to 6 parts cocamidopropyl betaine;

1 to 5 parts of polydimethylsiloxane;

0.8 to 1.5 parts of cocamide MEA;

0.8 to 2.0 parts of ethylene glycol distearate;

0.1 to 0.4 parts of guar gum hydroxypropyltrimonium chloride;

0.1 to 0.5 parts of sodium chloride;

0.05 to 0.2 parts of disodium EDTA.

In a fourth aspect, this application provides a method for preparing a hair shampoo product, including:

Add the evenly dispersed guar gum hydroxypropyltrimethylammonium chloride, disodium EDTA, and citric acid into a water bath at 80 to 85°C;

Then add ammonium laureth sulfate, ammonium laureth sulfate, and cocamidopropyl betaine to the water bath and dissolve evenly;

Then add cocamide MEA and ethylene glycol distearate to the water bath and keep warm for 15-25 minutes;

Then lower the temperature to below 45°C, add polydimethylsiloxane, sodium chloride, and scalp care anti-hair loss composition to the water bath; stir until evenly dissolved.

Description of drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required to be used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present application and therefore do not should be viewed as a limitation of scope.

Figure 1 shows a histogram of the relative width test results of the dorsal aorta vessel (DA) in zebrafish embryos;

Figure 2 shows a histogram of soothing efficacy test results;

Figure 3 shows a histogram of repair efficacy test results;

Figure 4 shows a histogram of relative expression levels of zebrafish 5α-reductase gene.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present application, not all of them. Embodiments.

Accordingly, the following detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application but is merely representative of selected embodiments of the present application. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of this application. The shampoo raw materials and reagents used in this application, unless otherwise specified, are all raw materials and reagents that can be obtained from commercial channels such as conventional markets.

The embodiment of the present application provides a scalp care and anti-hair loss composition. In terms of mass percentage, the scalp care and anti-hair loss composition includes:

Ginger juice 1.0% ~ 35%, allantoin 0.01% ~ 2.0%, panthenol 0.1% ~ 10.0%, Platycladus orientalis leaf extract 0.1% ~ 5%, hydrolyzed chitin 0.005% ~ 0.1%, salicylic acid 0.01% ~0.5%; the balance is water.

The anti-hair loss composition for scalp care provided by this application has synergistic cooperation between each component to achieve a synergistic effect; the role of each component is maximized, from multiple pathways of scalp hair follicles and scalp inflammation to multi-dimensional care. Scalp, make the scalp more nutritious, make the scalp healthier, thereby achieving the best and ideal state of preventing hair loss, and ultimately achieve the effect of preventing hair loss.

Applying the scalp care and anti-hair loss composition of the present application to a hair shampoo product can make the hair care product achieve a hair care and anti-hair loss effect. Thus, it can replace conventional anti-hair loss chemotherapy drugs and medical treatments, embodying the concept of convenience, health and safety.

In other embodiments of the present application, the method of using the above-mentioned scalp care and anti-hair loss composition includes: directly compounding the scalp care and anti-hair loss composition into a hair shampoo product for daily shampooing; convenient, simple and fast .

Further, in some embodiments of the present application, in terms of mass percentage, the anti-hair loss composition with scalp care includes:

Ginger juice 5% to 20%, allantoin 0.05% to 1.0%, panthenol 0.2% to 5%, Platycladus orientalis leaf extract 0.3% to 3%, hydrolyzed chitin 0.01% to 0.08%, salicylic acid 0.05% ~0.3%; the balance is water.

Further, in some embodiments of the present application, in terms of mass percentage, the anti-hair loss composition with scalp care includes:

Ginger juice 12%, allantoin 0.3%, panthenol 1.0%, Platycladus orientalis leaf extract 0.9%, hydrolyzed chitin 0.016%, salicylic acid 0.1%, the balance is water.

Further, in some embodiments of the present application, the active ingredients in ginger juice include: gingerols, parazingerones, zingiberones, and gingerdiones.

In some embodiments of the present application, the above-mentioned ginger juice may be derived from ginger.

Ginger is the fresh rhizome of the ginger plant. Gingerol is an active ingredient in ginger and mainly includes gingerols, parazingerones, zingingerone, gingerdione, etc. Its chemical components all contain 3-methoxy-4-hydroxyphenyl functional groups. Ginger has anti-inflammatory and antifungal properties and can be used to treat infections caused by Phytophthora fungus, Pityrosporum pityrosporum, etc. In this application plan, ginger juice is compounded with other components to treat seborrhea and dandruff, achieving a synergistic effect, which can effectively prevent hair loss and suppress scalp itching.

Further, in some embodiments of the present application, allantoin is an amphoteric compound that can combine with a variety of substances to form a complex salt, which has the functions of protecting against light, sterilizing and antiseptic, analgesic, and antioxidant, and can keep the skin hydrated, moisturized, and It is soft and is a special additive for beauty and hairdressing cosmetics. It is widely used as an additive in freckle cream, acne liquid, shampoo, soap, toothpaste, shaving lotion, hair conditioner, astringent, anti-sweat and deodorant lotion, etc. Cosmetics added with allantoin have the functions of protecting tissues, being hydrophilic, absorbing water, and preventing moisture loss. Lipsticks and facial creams added with allantoin can make the skin and lips soft, elastic, and have a beautiful luster. Allantoin promotes tissue growth, cell metabolism, and softens cuticle proteins. For example, the magic care moisturizing and gentle exfoliating lip mask's functions of softening cuticles, moisturizing and increasing gloss are mainly the functions of allantoin.

This application plan creatively combines allantoin with other components and uses it in hair cream, hair ointment, shampoo and other hair washing products, achieving a synergistic effect, achieving a protective effect on the hair, and making the hair more effective. The hair does not split and grows continuously.

Furthermore, in some embodiments of the present application, panthenol can be used as a moisturizer, which can increase the hydration of the stratum corneum, reduce TEWL, improve skin roughness and soothe irritated skin. In addition, it can activate fibroblast proliferation and accelerate epithelial cell regeneration, so it can help wound healing.

The scheme of this application creatively combines panthenol with other components for use in hair washing and care, achieving a synergistic effect, which can well enhance the toughness of hair and reduce damage caused by mechanical processes (such as combing hair); Maintains moisture content in hair to prevent dry and frizzy hair.

Further, in some embodiments of the present application, the active ingredients in Platycladus orientalis leaf extract include: Cypress biflavonoids, New Cryptomeria cyperus biflavonoids, Cryptomata biflavonoids, quercetin, quercetin, myricetin, Kaempferol.

Further, in some embodiments of the present application, the main components of Platycladus orientalis leaves are tannins and flavonoids. The flavonoids include Cypress biflavonoids, New Cryptomeria biflavonoids, Arborvitae biflavonoids, quercetin, and quercetin. , myricetin, kaempferol, etc., among which quercetin has the highest content, and tannins are tannins. The leaves of Platycladus arborvitae also contain volatile oil components of stone chambers, and the highest content is 2,2-tetramethyl -3,7-cyclodecadiene-1-methanol and cedrol, in addition to cedarwood, thujone, α-pinene, β-pinene, pine acetate grease, isocaryophyllene, α-caryophyllene, etc. .

Further, in some embodiments of the present application, Platycladus orientalis leaf extract has an inhibitory effect on Staphylococcus aureus, Escherichia coli, catarrhalis, Streptococcus A, pneumococcus, Shigella dysenteriae, Typhimurium, etc., and at the same time, It also has an inhibitory effect on the growth of Mycobacterium tuberculosis. The phenolic substances in the extract of Platycladus orientalis leaves have anti-human pathogenic fungi and can better inhibit Candida activity. The inhibitory test of Platycladus orientalis extract on toe edema in rats and the inhibition of urocanase have shown that it has anti-inflammatory effects.

This application plan creatively combines Platycladus orientalis extract with other components and uses it in hair care products to achieve a synergistic effect and can improve hair follicles and scalp inflammation.

Furthermore, in some embodiments of the present application, hydrolyzed chitin is a completely biodegradable natural organic matter, a deacetylation product of chitin, has a certain antibacterial effect, and is a relatively ideal antibacterial agent. The raw material has excellent inhibitory ability against Malassezia. At the same time, the aqueous solution is cationic, has high affinity for hair and scalp, has a certain conditioning effect, and has no accumulation effect.

The scheme of this application creatively combines hydrolyzed chitin with other components and uses them in hair care products to achieve a synergistic effect and can improve hair follicles and scalp inflammation.

Furthermore, in some embodiments of the present application, salicylic acid can dissolve the structural substances between the cuticles and cause the cuticles to fall off, so it can remove the accumulated thick cuticles and promote metabolism. If the epidermal cells are fresh and full of When the vitality of young cells is high, the cell vitality is stronger. Salicylic acid is fat-soluble and can penetrate into the deep layers of pores along the sebaceous glands that secrete oil. It is helpful to dissolve the old and accumulated stratum corneum in the pores and improve the clogging of the pores. Therefore, it can block the formation of acne and reduce the size of the pimples. pores. Salicylic acid acts on hair follicle wall cells and can help clear blocked hair follicles and correct abnormal cell shedding. It can prevent pores from being blocked for minor acne and is most effective for blackheads. It can reduce abnormal shedding of hair follicle walls. , to prevent the occurrence of new lesions.

This application plan creatively combines salicylic acid with other components and uses it in hair care products to achieve a synergistic effect, which can improve hair follicles, promote hair follicle growth, and reduce hair follicle shedding.

Further, illustratively, in some embodiments of the present application, in terms of mass percentage, the scalp care anti-hair loss composition includes:

Ginger juice 5%, 6%, 8%, 10%, 15%, 18%, 20% or the range between any two of the aforementioned values;

Allantoin 0.05%, 0.06%, 0.08%, 0.1%, 0.5%, 0.6%, 0.8%, 1.0%, or the range between any two of the aforementioned values;

Panthenol 0.2%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 4%, 5% or the range between any two of the aforementioned values;

Platycladus orientalis leaf extract 0.3%, 0.5%, 0.8%, 1%, 1.5%, 1.8%, 2%, 2.5%, 2.8%, 3% or the range between any two of the aforementioned values;

Hydrolyzed chitin 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08% or the range between any two of the aforementioned values;

Salicylic acid 0.05%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.26%, 0.28%, 0.3% or the range between any two of the aforementioned values;

The remainder is water.

Further, in some embodiments of the present application, the anti-hair loss composition with scalp care does not contain purslane extract.

In some embodiments of the present application, the preparation method of the above-mentioned anti-hair loss composition for scalp care includes: mixing and stirring the above-mentioned components evenly, that is, the anti-hair loss composition for scalp care.

Further, in some embodiments of the present application, the application of the anti-hair loss composition with scalp care provided in any of the aforementioned embodiments in shampoo products is provided.

Some embodiments of the present application provide a hair shampoo product, including the scalp care and anti-hair loss composition provided in any of the aforementioned embodiments.

Further, in some embodiments of the present application, hair shampoo products include:

10 to 15 parts of a scalp care and anti-hair loss composition;

10 to 18 parts of ammonium laureth sulfate;

1 to 5 parts of ammonium lauryl sulfate;

1 to 6 parts cocamidopropyl betaine;

1 to 5 parts of polydimethylsiloxane;

0.8 to 1.5 parts of cocamide MEA;

0.8 to 2.0 parts of ethylene glycol distearate;

0.1 to 0.4 parts of guar gum hydroxypropyltrimonium chloride;

0.1 to 0.5 parts of sodium chloride;

0.05 to 0.2 parts of disodium EDTA.

Exemplarily, in some embodiments of the present application, hair shampoo products include:

10, 11, 12, 13, 14 or 15 parts of a scalp care and anti-hair loss composition;

10, 11, 12, 13, 14, 15, 16, 17 or 18 parts of ammonium laureth sulfate;

1, 2, 3, 4 or 5 parts ammonium lauryl sulfate;

1, 2, 3, 4, 5 or 6 parts of cocamidopropyl betaine;

1, 2, 3, 4 or 5 parts dimethicone;

0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 parts of cocamide MEA;

0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.6, 1.8, or 2.0 parts of ethylene glycol distearate;

0.1, 0.2, 0.3, or 0.4 parts of guar gum hydroxypropyltrimonium chloride;

0.1, 0.2, 0.3, 0.4 or 0.5 parts sodium chloride;

0.05, 0.06, 0.08, 0.1, 0.12, 0.15, 0.18 or 0.2 parts disodium EDTA.

In other optional embodiments of the present application, the above-mentioned shampoo product also includes: 0.08-0.1 parts by weight of preservatives, 0.01-0.05 parts by weight of citric acid, 0.2-0.5 parts by weight of essence; and the remaining amount of water (make up with water to 100 parts by weight).

Some embodiments of the present application provide a method for preparing a hair shampoo product, including:

Add the evenly dispersed guar gum hydroxypropyltrimethylammonium chloride, disodium EDTA, and citric acid into a water bath at 80 to 85°C;

Then add ammonium laureth sulfate, ammonium laureth sulfate, and cocamidopropyl betaine to the water bath and dissolve evenly;

Then add cocamide MEA and ethylene glycol distearate to the water bath and keep warm for 15-25 minutes;

Then lower the temperature to below 45°C, add polydimethylsiloxane, sodium chloride, and scalp care anti-hair loss composition to the water bath; stir until evenly dissolved.

Further, in some embodiments of the present application, the preparation method of the shampoo product includes the following steps:

Step S1: Add the evenly dispersed guar gum hydroxypropyltrimethylammonium chloride, disodium EDTA, and citric acid into a water bath at 80 to 85°C.

Further, in some embodiments of the present application, uniformly dispersed guar hydroxypropyltrimethylammonium chloride, EDTA disodium, and citric acid are added to 80°C, 81°C, 82°C, 83°C, 84°C or 85°C water bath.

Illustratively, in some embodiments of the present application, in a water bath, the water is heated to 80°C to 85°C and then constant temperature, and then the guar gum hydroxypropyltrimethylammonium chloride and EDTA that have been dispersed in water are added. Disodium, citric acid.

Step S2: Then add ammonium laureth sulfate, ammonium laureth sulfate, and cocamidopropyl betaine to the water bath obtained in step S1 and dissolve them evenly.

Illustratively, in some embodiments of the present application, ammonium laureth sulfate, ammonium laureth sulfate, and cocamidopropyl betaine are added to the water bath obtained in step S1 to completely dissolve them.

Step S3: Then add cocamide MEA and ethylene glycol distearate to the water bath obtained in step S2, and keep it warm for 15-25 minutes.

Exemplarily, in some embodiments of the present application, cocamide MEA and ethylene glycol distearate are added to the water bath obtained in step S2, and the temperature is kept constant for 15 min, 16 min, 17 min, 18 min, 19 min, 20 min, 21 min, 22min, 23min, 24min or 25min minutes.

Step S4: Then cool the water bath obtained in step S3 to below 45°C, add polydimethylsiloxane, sodium chloride, and scalp care anti-hair loss composition into the water bath; stir until uniformly dissolved.

Exemplarily, in some embodiments of the present application, the water bath obtained in step S3 is cooled to below 45°C, the remaining polydimethylsiloxane, sodium chloride, preservatives and essence are added, and finally scalp care is added anti-hair loss composition, continue stirring for 15 minutes.

This application adds a scalp care anti-hair loss composition to the shampoo product. The anti-hair loss composition for scalp care in the present invention repairs scalp function and makes the scalp healthier by caring for the scalp, thereby achieving the effect of preventing or reducing hair loss.

Some specific examples are listed below to better illustrate this application.

Example

A scalp care and anti-hair loss composition is provided, the specific composition of which is shown in Table 1.

Table 1

Comparative Example

A composition is provided, the specific composition of which is shown in Table 2.

Table 2

The properties of the compositions provided in each example or each comparative example were tested.

Experimental example

The compositions provided in Examples 1 to 7 and Comparative Examples 1 to 7 were tested in four dimensions using the zebrafish test model: repair promotion rate, neutrophil aggregation resistance rate, 5α reductase gene expression inhibition rate, and vasodilation rate. test.

1. Zebrafish embryo tail fin repair promotion test: Use the zebrafish embryo tail fin removal model for testing. 24 fish embryos with caudal fins removed were exposed to 0.01g/L test sample solution, and a blank control group, a positive control group and a model control group were set up at the same time. After 48 hours of exposure, the fish embryos were photographed under a microscope, and the regenerated tail fin length was measured and carried out. Statistical Analysis.

In the formula: S - the average length of the tail fin of fish embryos in the test substance treatment group;

M—the average length of embryonic tail fins of fish in the model control group;

B—The average tail fin length of fish embryos in the blank control group.

Calculate the mean value and standard error of each test group, and the statistical processing results are expressed as mean ± standard error. Use statistical software to conduct variance analysis on the data, conduct a two-tailed T test on the length of the caudal fin between the test object group and the model control group, and obtain the p value. p<0.05 indicates significant difference.

The higher the repair promotion rate, the better the repair promotion effect of the composition.

2. Zebrafish 5α-reductase gene expression test: 36 5-day-old zebrafish were divided into 3 groups and exposed to 0.05g/L test sample solution, and a blank control group was set up. After 24 hours of exposure, RNA was extracted from the zebrafish. , cDNA synthesis, real-time PCR amplification, using β-actin as the housekeeping gene and Ct as the amplification result, the relative expression of the 5α reductase gene was calculated and statistically analyzed.

In the formula: 2 ^-ΔΔCt —relative gene expression.

Calculate the mean value and standard error of each test group, and the statistical processing results are expressed as mean ± standard error. Use statistical software to conduct variance analysis on the data, conduct a two-tailed T test on the relative expression of the target gene between the test substance group and the blank control group, and obtain the p value. p<0.05 indicates significant difference.

The greater the value of the 5α reductase gene expression inhibition rate, the better the composition's ability to inhibit androgen and reduce androgenic alopecia.

3. Neutrophil aggregation inhibition test in zebrafish embryos: The neutrophil aggregation model caused by copper sulfate-induced damage to neuromast cells in the lateral line region of zebrafish embryos was used for testing. 24 fish embryos were exposed to 0.16mg/L copper sulfate pentahydrate and 0.05g/L test sample solution, and a blank control group, a positive control group and a model control group were set up at the same time. After 40 minutes of exposure, the fish embryos were fixed and Sudan black. After staining, the number of neutrophils in the lateral line area was counted and analyzed statistically.

In the formula:

S—the average number of neutrophils in embryonic fish embryos treated with the test substance, the unit is grain per tail (grain/tail);

M—the average number of neutrophils in fish embryos in the model control group, unit is grain per tail (grain/tail);

B—The average number of neutrophils in fish embryos in the blank control group, the unit is grain per tail (grain/tail).

Calculate the mean value and standard error of each test group, and the statistical processing results are expressed as mean ± standard error. Use statistical software to conduct variance analysis on the data, conduct a two-tailed T test on the number of neutrophils between the test group and the model control group, and obtain the p value. p<0.05 indicates significant difference.

The higher the neutrophil aggregation resistance rate, the better the soothing performance of the composition.

4. Zebrafish embryo blood vessel dilation efficacy test: 12 3-day-old Fli1:EGFP transgenic zebrafish embryos were exposed to 0.01g/L test sample solution, and a blank control group was set up. After 3 hours of exposure, each site was photographed under a microscope. Width measurements were taken, averaged and statistically analyzed. Dorsal aortic vessels in caudal zebrafish embryos. Then the software was used to average the five width measurements of the dorsal aortic vessels of each fish embryo and perform statistical analysis.

In the formula:

S—the average blood vessel width of the dorsal aorta of fish embryos in the test substance treatment group;

B—The average blood vessel width of the dorsal aorta of fish embryos in the blank control group;

Calculate the mean value and standard error of each test group, and the statistical processing results are expressed as mean ± standard error. Use statistical software to conduct variance analysis on the data, conduct a two-tailed T test on the width of the dorsal aorta between the test substance group and the blank control group, and obtain the p value. p<0.05 indicates significant difference.

The higher the blood vessel dilation rate, the better the blood vessel dilation performance of the composition.

The test results of the 4 dimensions of zebrafish are shown in Table 3.

table 3

The zebrafish 4-dimensional test results are used to comprehensively evaluate the scalp care and then evaluate the anti-hair loss effect.

It can be seen from the test in Table 3 that Examples 1 to 7 have certain promoting effects in the four dimensions of repair promotion rate, neutrophil aggregation resistance rate, 5α reductase gene expression inhibition rate, and vasodilation rate. It can be seen that the solution of this application can repair the scalp from multiple dimensions, make the scalp more nutritious, make the scalp healthier, achieve the best ideal state of preventing hair loss, and ultimately achieve the effect of preventing hair loss.

Comparison with the test results of Comparative Examples 1 to 7 shows that the synergistic effect between the components of the embodiment repairs the scalp from multiple dimensions, makes the scalp more nutritious, makes the scalp healthier, and achieves the best ideal state for preventing hair loss. , ultimately achieving the effect of preventing hair loss.

Compared with Example 4, the repair promotion rate, neutrophil aggregation resistance rate, 5α reductase gene expression inhibition rate, and vasodilation rate in each comparative example are all significantly lower than those in Example 4; it can be seen that, The anti-hair loss effect of the comparative example is significantly lower than the anti-hair loss effect of the examples. This shows that the synergistic cooperation between the components of the composition of the present application produces a synergistic effect, repairing the scalp from multiple dimensions, making the scalp more nutritious, making the scalp healthier, and achieving the effect of preventing hair loss. The optimal and ideal state ultimately achieves the effect of preventing hair loss. Furthermore, comparing Comparative Example 7 with Example 4 of the present application, it can be seen that when other ingredients, such as purslane extract, etc., are added to the composition of the present application, it will have an inhibitory effect on the efficacy of the composition of the present application.

Therefore, each component of the composition of the present application cooperates with each other synergistically, and components cannot be added or subtracted arbitrarily, otherwise it may cause inhibitory effects.

【Application example】

The composition provided in the above Example 4 was applied to a shampoo product as a sample treatment group to detect the anti-hair loss effect of the shampoo product.

The formula of the shampoo of the composition provided in Example 4 is shown in Table 4 below.

Table 4

water to 100g Ammonium laureth sulfate 11.0g Ammonium lauryl sulfate 8.0g Cocamidopropyl betaine 5.0g Polydimethylsiloxane 4.0g Cocamide MEA 1.2g Ethylene glycol distearate 1.5g Guar Gum Hydroxypropyl Trimonium Chloride 0.3g Sodium chloride 0.3g Disodium EDTA 0.1g preservative 0.1g citric acid 0.05g essence 0.5g Composition provided in Example 4 14.516g

A shampoo formula without adding the above-mentioned scalp care anti-hair loss composition was used as a blank comparative example. The specific composition is shown in Table 5.

table 5

water to 100g Ammonium laureth sulfate 11.0g Ammonium lauryl sulfate 8.0g Cocamidopropyl betaine 5.0g Polydimethylsiloxane 4.0g Cocamide MEA 1.2g Ethylene glycol distearate 1.5g Guar Gum Hydroxypropyl Trimonium Chloride 0.3g Sodium chloride 0.3g Disodium EDTA 0.1g preservative 0.1g citric acid 0.05g essence 0.5g

Shampoo product preparation method:

In a water bath, heat the water to 80-85°C and maintain the temperature, then add the guar gum hydroxypropyltrimonium chloride, disodium EDTA, and citric acid that have been dispersed in water before adding laureth. After ammonium sulfate, ammonium lauryl sulfate, and cocamidopropyl betaine are completely dissolved, add cocamide MEA and ethylene glycol distearate. After keeping the temperature constant for 20 minutes, cool down to below 45°C. , then add the remaining polydimethylsiloxane, sodium chloride, preservatives and flavors, and finally add the composition of Example 4 (do not add it when using the comparative example), and continue stirring for 15 minutes.

Test the performance of the above shampoo products.

1. By testing 20 volunteers (10 for each formula, aged 25 to 60 years old, with dispersed, non-health-related hair loss and thin, lifeless hair), the aforementioned Example 4 and the blank pair were tested respectively. For the proportion of samples, the test anti-shedding effect is scored according to the evaluation method, and the final result is the average score. Use the shampoo to wash your hair twice a week for a total of 12 weeks. Compare the anti-hair loss effect before use and after 12 weeks of use. Follow up the test to examine the hair loss improvement of each volunteer.

Test scoring standards: The scores are divided into five levels: 1, 2, 3, 4, and 5. Among them, 1 means that the hair loss situation has not been improved at all, 2 means that the hair loss situation has not improved significantly, 3 means that the hair loss situation has been slightly improved, and 4. 1 means the hair loss situation has been improved well, 5 means the hair loss situation has improved significantly, and the improvement is evaluated at intervals of 0.1 points. After the final trial for 12 weeks, the average score of 10 people in each group was combined. The calculation results are shown in Table 6. Test score results.

Table 6 Formulas and test results of the shampoo provided in Example 4 and Blank Comparative Example

As can be seen from Table 6, the anti-hair loss composition for scalp care in Example 4 can achieve the same effect in shampoo, making the scalp healthier, ideally improving hair loss, and ultimately achieving the effect of preventing hair loss.

The anti-hair loss effect of the blank comparative example is significantly lower than the anti-hair loss effect of the solution in Example 4.

2. Through the zebrafish multi-dimensional test Example 4 sample treatment group and blank comparison example.

(1) Relative width test of the dorsal aorta (DA) of zebrafish embryos: 12 3-day-old Fli1:EGFP transgenic zebrafish embryos were exposed to 0.01g/L test sample solution, and a blank control group was set at the same time for 3 hours. Then, the width of each same spot was photographed under a microscope, the average value was taken, and statistical analysis was performed. Dorsal aortic vessels in caudal zebrafish embryos. Then software was used to average the five width measurements of the dorsal aortic vessels of each fish embryo and perform statistical analysis.

The test results are shown in Figure 1 of the instruction manual.

As can be seen from Figure 1: the blood vessel dilation rate of the sample treatment group reached 14% and p<0.05, indicating that it can significantly expand the zebrafish embryo aorta blood vessels, indicating that the sample group has the effect of dilating blood vessels.

(2) Soothing efficacy test: Use neutrophil number characterization: Zebrafish embryo neutrophil aggregation inhibition test: Use copper sulfate to induce neutrophil aggregation model caused by damage to neuromast cells in the lateral line area of zebrafish embryos. 24 fish embryos were exposed to 0.16mg/L copper sulfate pentahydrate and 0.05g/L test sample solution, and a blank control group, a positive control group and a model control group were set up at the same time. After 40 minutes of exposure, the fish embryos were fixed and Sudan black. After staining, the number of neutrophils in the lateral line area was counted and analyzed statistically.

The test results are shown in Figure 2 of the instruction manual.

It can be seen from Figure 2 that the aggregation inhibition rate of the sample treatment group is 23% lower than that of the blank control group and p<0.05. The sample treatment group can significantly inhibit the aggregation of zebrafish embryonic neutrophils, indicating that the sample group has a soothing effect.

(3) Repair efficacy test: Use the relative length of zebrafish tail fin to characterize: use the zebrafish embryo tail fin resection model for testing. 24 fish embryos with caudal fins removed were exposed to 0.01g/L test sample solution, and a blank control group, a positive control group and a model control group were set up at the same time. After 48 hours of exposure, the fish embryos were photographed under a microscope, and the regenerated tail fin length was measured and carried out. Statistical Analysis.

The test results are shown in Figure 3 of the instruction manual.

It can be seen from Figure 3 that the repair promotion rate of zebrafish embryo tail fins in the sample treatment group is 30% and p<0.05. The sample treatment group can significantly promote the regeneration of zebrafish embryo tail fins, indicating that the sample has repair performance.

(4) Zebrafish 5α-reductase gene expression test: 36 5-day-old zebrafish were divided into 3 groups and exposed to 0.05g/L test sample solution, and a blank control group was set up. After 24 hours of exposure, the zebrafish were tested. RNA extraction, cDNA synthesis, real-time PCR amplification, using β-actin as the housekeeping gene and Ct as the amplification result, calculated the relative expression of the 5α-reductase gene and performed statistical analysis.

The test results are shown in Figure 4 of the instruction manual.

It can be seen from Figure 4 that the sample treatment group has lower expression levels of srd5a1 and srd5a2b genes, indicating that the expression inhibition rate of zebrafish 5α-reductase type I gene (srd5a1) and zebrafish 5α-reductase type II gene (srd5a2b) The expression inhibition rate is higher than that of the blank control group, indicating that the sample has the ability to inhibit androgen and reduce androgenic alopecia.

In summary, each performance of the sample of Example 4 of the present application is better than that of the blank control group. This can be explained that the anti-hair loss composition for scalp care of the present application can achieve the same effect as the composition when used in shampoo, making the scalp more healthy. Healthy, ideal for improving hair loss, and ultimately preventing hair loss.

The above-described embodiments are part of the embodiments of the present application, but not all of the embodiments. The detailed description of the embodiments of the application is not intended to limit the scope of the application as claimed, but rather to represent selected embodiments of the application. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of this application.

Claims (10)

1. A scalp care and anti-hair loss composition, characterized in that, in terms of mass percentage, the scalp care and anti-hair loss composition includes: Ginger juice 1.0% ~ 35%, allantoin 0.01% ~ 2.0%, panthenol 0.1% ~ 10.0%, Platycladus orientalis leaf extract 0.1% ~ 5%, hydrolyzed chitin 0.005% ~ 0.1%, salicylic acid 0.01% ~0.5%; the balance is water. 2. The anti-hair loss composition with scalp care according to claim 1, characterized in that, in terms of mass percentage, the anti-hair loss composition with scalp care includes: Ginger juice 5% to 20%, allantoin 0.05% to 1.0%, panthenol 0.2% to 5%, Platycladus orientalis leaf extract 0.3% to 3%, hydrolyzed chitin 0.01% to 0.08%, salicylic acid 0.05% ~0.3%; the balance is water. 3. The anti-hair loss composition with scalp care according to claim 1, characterized in that, in terms of mass percentage, the anti-hair loss composition with scalp care includes: Ginger juice 12%, allantoin 0.3%, panthenol 1.0%, Platycladus orientalis leaf extract 0.9%, hydrolyzed chitin 0.016%, salicylic acid 0.1%, the balance is water. 4. The scalp care and anti-hair loss composition according to any one of claims 1 to 3, characterized in that, The active ingredients in the ginger juice include: gingerols, parazingerones, zingiberone, and gingerdione. 5. The scalp care and anti-hair loss composition according to any one of claims 1 to 3, characterized in that, The active ingredients in the Platycladus orientalis leaf extract include: Cypress biflavonoids, Cryptomeria cypress biflavonoids, Arborvitae biflavonoids, quercetin, quercetin, myricetin, and kaempferol. 6. The scalp care and anti-hair loss composition according to any one of claims 1 to 3, characterized in that, The scalp care and anti-hair loss composition does not contain purslane extract. 7. Application of the scalp care and anti-hair loss composition according to any one of claims 1 to 6 in shampoo products. 8. A hair shampoo product, characterized by comprising the scalp care and anti-hair loss composition according to any one of claims 1 to 6. 9. The shampoo product according to claim 8, characterized in that, in parts by weight, The shampoo products include: 10 to 15 parts of the scalp care and anti-hair loss composition; 10 to 18 parts of ammonium laureth sulfate; 1 to 5 parts of ammonium lauryl sulfate; 1 to 6 parts cocamidopropyl betaine; 1 to 5 parts of polydimethylsiloxane; 0.8 to 1.5 parts of cocamide MEA; 0.8 to 2.0 parts of ethylene glycol distearate; 0.1 to 0.4 parts of guar gum hydroxypropyltrimonium chloride; 0.1 to 0.5 parts of sodium chloride; 0.05 to 0.2 parts of disodium EDTA. 10. The preparation method of the shampoo product according to claim 9, characterized in that it includes: Add the evenly dispersed guar gum hydroxypropyltrimethylammonium chloride, disodium EDTA, and citric acid into a water bath at 80 to 85°C; Then add ammonium laureth sulfate, ammonium laureth sulfate, and cocamidopropyl betaine to the water bath and dissolve them evenly; Then add cocamide MEA and ethylene glycol distearate to the water bath and keep warm for 15-25 minutes; Then, the temperature is lowered to below 45° C., and polydimethylsiloxane, sodium chloride, and the scalp care and anti-hair loss composition are added to the water bath; stir until uniformly dissolved.