CN117679479A - A kind of traditional Chinese medicine compound preparation extract and preparation method - Google Patents

Abstract

The invention discloses a traditional Chinese medicine compound preparation extract and a preparation method thereof, belonging to the technical field of traditional Chinese medicines. The invention prepares water decoction by heating and decocting three traditional Chinese medicine compositions containing glabrous greenbrier rhizome, dandelion, honeysuckle, baical skullcap root or any glabrous greenbrier rhizome, and then uses 63%, 76% and 81% ethanol for overnight precipitation treatment to obtain alcohol solution and alcohol sediment, respectively concentrating or drying the alcohol solution and the alcohol sediment to obtain crude extract, and then obtaining the extract containing various active chemical components after separating and purifying the crude extract. The traditional Chinese medicine extract provided by the invention has the effects of resisting bacteria, diminishing inflammation, preventing corrosion and guaranteeing quality, and experiments prove that the combined water decoction of glabrous greenbrier rhizome, dandelion, honeysuckle and baical skullcap root has strong inhibition effect on escherichia coli, staphylococcus aureus and bacillus subtilis, can protect organisms from being damaged to a certain extent, and has detoxification effect.

Description

A kind of traditional Chinese medicine compound preparation extract and preparation method

Technical field

The invention relates to a traditional Chinese medicine compound preparation extract and a preparation method, and belongs to the technical field of traditional Chinese medicine.

Background technique

Smilax glabra Roxb. is the dry rhizome of the Liliaceae plant Smilax glabra Roxb. It is a perennial evergreen climbing shrub that mostly grows on hillsides or understory. Distributed in China's Gansu (southern) and provinces and regions south of the Yangtze River Basin, to Taiwan, Hainan Island and Yunnan. It is also distributed in Vietnam, Thailand and India. Mainly used to treat syphilis and skin diseases. Modern pharmacological research shows that Tuhuoquan has a wide range of biological activities. The main active ingredients are flavonoids, terpenes, organic acids, sterols, saponins, etc. It has pharmacological effects such as immunosuppression and has good medicinal value. In addition, due to its rich nutritional content, low toxic and side effects, and high economic value, Tuhuoling is widely used in many fields such as disease prevention and treatment, food, breeding, and skin care products, and has good application prospects.

Dandelion is the dried whole grass of Taraxacum mongolicum Hand.-Mazz., Taraxacum borealisinense Kitam., or several species of the same genus. Also known as dandelion grass, golden grass, mother-in-law grass, etc., it can be found in most parts of China. It is widely grown in hillside grasslands, roadsides, fields, river beaches, etc. in medium and low altitude areas. It is a common wild vegetable and Chinese herbal medicine in China. It has the functions of clearing away heat and detoxifying, reducing swelling and dissipating knots, diuresis, treating stranguria, and beautifying. It is mainly used to treat carbuncle, sores, and mastitis. Modern pharmacological research has confirmed that this product has choleretic, hepatoprotective, antibacterial and anti-inflammatory properties (rheumatoid arthritis, stomatitis, periodontitis, gynecological inflammation, etc.), diuresis, antioxidant free radicals, anti-cancer and enhances the body's immune function. It has clinical applications. The scope continues to expand. In addition, dandelion also contains a variety of health-care nutritional active ingredients such as glucose, vitamins, carotene and rich trace elements. Therefore, it has very important nutritional value. The Ministry of Health has listed it as a medicinal and food product.

Honeysuckle, the correct name is Lonicera japonica Thunb. It is the dried flower bud or newly bloomed flower of Lonicera japonica. It is a perennial semi-evergreen twining and stolid shrub that mostly grows wild in relatively humid areas. All provinces in China are Distributed, mainly in Shandong, Shaanxi, Henan, Hebei, Hubei, Jiangxi, Guangdong and other places. It has the effects of clearing away heat and detoxification, cooling blood and removing blood stasis. It is mainly used to treat exogenous wind-heat, the onset of plague, sores, boils, redness, swelling and heat pain. , fecal pus and blood", etc. Honeysuckle contains a variety of trace elements and chemical components necessary for the human body. It also contains a variety of active enzyme substances that are beneficial to the human body. It has good effects of anti-aging, preventing cancer, and being light-weight and healthy. It is determined to be A variety that can be used both as medicine and food. Dandelion and honeysuckle are both used to eliminate carbuncles and ulcers. Honeysuckle has greater effects than dandelion.

Scutellaria baicalensis Georgi., also known as camellia root and earth gold tea root, is a perennial herbaceous plant of the genus Scutellaria of the family Lamiaceae. It grows wild in sunny and dry places such as mountain tops, hillsides, forest edges, and roadsides. It is mainly distributed in China, Russia, Mongolia, North Korea and Japan; my country's Heilongjiang, Liaoning, Inner Mongolia, Hebei, Henan, Gansu, Shanxi, Shandong, Sichuan and other places are also widely planted. It has the effects of clearing away heat and drying dampness, purging fire and detoxifying, stopping bleeding, and anti-fetus. It is mainly used to treat damp-heat fullness, diarrhea, jaundice, lung-heat cough, high fever and polydipsia, blood-heat vomiting epistaxis, carbuncle and sore, uneasy fetal movement, etc. The rhizome of Scutellaria baicalensis is a cooling, antipyretic and anti-inflammatory drug with antibiotic effects for external use. The stems can be used to extract aromatic oil. Modern pharmacological research shows that Scutellaria baicalensis contains a variety of active ingredients, including flavones, flavonoids, polysaccharides, volatile oils and trace elements, the most important of which are flavones and flavonoids. It has anti-inflammatory, antibacterial, anti-tumor, antioxidant, anti-aging, hypoglycemic and protective effects on the liver, nerves and heart. Astragalus is a plant that can be used as tea. The rhizome of Astragalus is used to make tea and drink in the mountainous areas of Hebei, China. It is called Scutellaria baicalensis tea, which is a different kind of tea. The tea brewed from the stems and leaves of Scutellaria baicalensis is golden in color, fresh in taste, and has health-care effects such as removing internal heat.

The above four traditional Chinese medicines are all heat-clearing and resolving hormones with the same origin as medicine and food. Chinese herbal medicines that clear away heat and detoxify play an important role in antibacterial, anti-inflammatory and antiseptic properties. Chinese herbal medicines can be taken not only internally but also externally, and have important application prospects in life and production. Medical workers of past generations have summed up their experience in long-term medical practice and selectively combined two or more traditional Chinese medicines. The study of drug compatibility is a new method, new direction, and new way to study the rules of prescription compatibility. The compatibility of traditional Chinese medicine can achieve twice the result with half the effort. This is the inevitable result of the components of traditional Chinese medicine and the interaction between each component. The combined use of drugs has more advantages than single drugs. If the dissolution rate of effective substances is increased, new chemical molecules may be produced, making the traditional Chinese medicine compound preparations have the advantages of multi-targeting and pleiotropic effects.

The research on medicinal and edible plant compound agents plays an important role in life and production, especially the antiseptic and quality-preserving effects of Chinese herbal plant extracts, which play a role in the antiseptic and quality-preserving process. Chinese herbal medicine plants can also play a health care role.

Contents of the invention

In order to solve the deficiencies of the existing technology, the present invention provides a traditional Chinese medicine compound preparation extract and a preparation method. The technical solutions adopted are as follows:

The object of the present invention is to provide a traditional Chinese medicine compound preparation, extract and preparation method. The method is a traditional Chinese medicine extract, which is extracted from a combination of the following four traditional Chinese medicines and any three traditional Chinese medicine combinations containing Smilax smilax.

1) Smilax poria;

2) Dandelion;

3) Honeysuckle;

4) Scutellaria baicalensis:

The traditional Chinese medicine Smilax poria is the dried rhizome of the plant; dandelion is the dried whole plant; honeysuckle is the dried flower bud or newly opened flower (with branches and leaves) of the honeysuckle plant; and skullcap is the dried root of the plant.

The preparation method of the extract is to heat and boil the three Chinese medicine combinations or four Chinese medicine compositions described in claim 1 to form a water decoction, and then use 63% ethanol, 76% ethanol, 81% After an overnight ethanol precipitation treatment, the alcohol soluble product and the alcohol precipitate are obtained, which are concentrated or dried respectively to obtain a crude extract. The crude extract is then separated and purified to obtain a Chinese medicine extract.

The method steps are as follows:

1) Pre-process any three Chinese medicines or four Chinese medicine compositions containing Smilax poria to obtain the pretreated Chinese medicine;

2) Boil the pretreated Chinese medicine obtained in step 1) into a water decoction, powder or medicinal packet;

3) The water decoction obtained in step 2) is subjected to liquid precipitation treatment with ethanol to obtain alcohol soluble products and alcohol precipitates;

4) Concentrate or dry the alcohol-soluble product and alcohol precipitate obtained in step 3) respectively to obtain a crude extract of traditional Chinese medicine;

5) Then use silica gel fast chromatography technology and high performance liquid chromatography technology to separate and purify the crude extract obtained in step 4) to obtain the traditional Chinese medicine extract.

Step 1) The traditional Chinese medicines are Smilax poria, dandelion, honeysuckle and skullcap, as well as any three traditional Chinese medicine combination preparations, suspensions, powders or medicine packets containing Smilax. The Chinese medicine composition is Smilax, dandelion, honeysuckle, Four 1:1:1:1 combinations of Scutellaria baicalensis and any three Chinese medicine combinations containing Smilax Poria cocos 1:1:1.

The pretreatment described in step 1) is to soak the traditional Chinese medicine composition in water. The soaking condition is room temperature for 2 hours. The amount of water added is 20 times the total dosage of the herbal medicine.

To make a water decoction as described in step 2), boil the pretreated Chinese medicine or Chinese medicine composition first, and then simmer it over low heat for 30 minutes. Collect the boiling liquid, then add 10 times of water, boil it first and then simmer over low heat. After 30 minutes, collect the boiling liquid, combine the two liquids, let it stand overnight, collect the supernatant first the next day, centrifuge the precipitate at 3000 rpm for 20 minutes to collect the supernatant, and concentrate the entire supernatant under boiling conditions at 100°C. to 400mL, and finally in a 100°C water bath, concentrate to 100mL water decoction and set aside.

The ethanol overnight precipitation treatment in step 3) is to divide 120 mL of water decoction concentrate into three parts, each part is 40 mL, and add 80 mL of 95% absolute ethanol to make it 63% ethanol, and add 40 mL of water decoction concentrate to To make 160 mL of 95% absolute ethanol into 76% ethanol, add 240 mL of 95% absolute ethanol to 100 mL of water decoction concentrate to make it into 81% ethanol. Leave it overnight at room temperature for 24 hours. Add three portions of the obtained alcohol solution and The alcohol precipitates combine to form an alcohol soluble mixture and an alcohol precipitate mixture.

Step 4) The Chinese medicine alcohol-soluble mixture and the alcohol-precipitated mixture were concentrated in a 100°C water bath to a paste, filtered under reduced pressure to form dry powder, dried in a drying oven at 80°C, and set aside.

The method of the present invention is suitable for compound preparations of traditional Chinese medicines, extracting active ingredients from traditional Chinese medicines, Chinese medicine decoctions or crude extracts.

Beneficial effects of the present invention:

1. The decoction prepared by using the traditional Chinese medicine composition, extract and preparation method provided by the invention is more in line with the requirements of the nature, taste, return and meridians of traditional Chinese medicine, and better interprets the compatibility of traditional Chinese medicine prescriptions with monarch, minister, adjuvant and make. Usage: Use usage to bring out the mutually reinforcing and mutually reinforcing effects between drugs.

2. Utilizing the preparation method of traditional Chinese medicine extracts provided by the present invention, it is possible to control multiple active chemical components of traditional Chinese medicine extracts to a certain extent, obtain Chinese medicine extracts containing multiple chemical components, and better reflect the effectiveness of traditional Chinese medicine decoctions. The efficacy and medicinal properties of the ingredients.

3. The traditional Chinese medicine extract provided by the present invention has antibacterial, anti-inflammatory, antiseptic and quality-preserving effects. It has been experimentally verified that the Chinese medicine extract prepared in the present invention has antibacterial effects and the effect of improving the body's immunity in vitro.

Detailed ways

The present invention will be further described below with reference to specific examples, but the present invention is not limited by the examples. After optimizing the compatibility ratio, based on the obtained compatibility ratio with better effect, the following compatibility combinations are listed for further explanation:

Example 1: Preparation of extracts of Smilax poria, dandelion, honeysuckle and skullcap compositions

Add 20 times of the total dosage of traditional Chinese medicine (120g) to four whole herbs: 30g of Smilax, 30g of Dandelion, 30g of Honeysuckle, and 30g of Scutellaria baicalensis, and soak them at room temperature for 2 hours to obtain the pretreated traditional Chinese medicine. Boil the obtained pretreated Chinese medicine for 30 hours at 100°C, filter the boiled liquid with 4 layers of gauze (normal pressure filtration), then add 10 times of water, simmer for 30 minutes, and filter the boiled liquid with 4 layers of gauze. Drug residue. Combine the two solutions and let sit overnight. The next day, take the supernatant, centrifuge the sediment (3000 rpm, 20 minutes), combine all the supernatants, boil and concentrate to 400 mL, then concentrate the supernatant in a 100°C water bath to 120 mL of water decoction, divide it into three parts, each 40 mL portion, add 80 mL 95% absolute ethanol to make it 63% ethanol; add 160 mL 95% absolute ethanol to make it 76% ethanol; add 240 mL 95% absolute ethanol to make it 81% ethanol, and leave it at room temperature overnight for 24 hours. , combine the three alcohol solubles and alcohol precipitates obtained to form an alcohol soluble product mixture and an alcohol precipitate mixture. The alcohol-soluble product mixture and the alcohol-precipitated product mixture were concentrated in a 100°C water bath to a paste, filtered under reduced pressure to form dry powder, dried in a drying oven at 80°C, and set aside.

Example 2: Preparation of extracts of Smilax poria, dandelion and honeysuckle compositions

Add 20 times of the total dosage of traditional Chinese medicine (90g) to three whole herbs: 30g of Smilax poria, 30g of dandelion, and 30g of honeysuckle, and soak them at room temperature for 2 hours to obtain the pretreated traditional Chinese medicine. Boil the obtained pretreated Chinese medicine for 30 hours at 100°C, filter the boiled liquid with 4 layers of gauze (normal pressure filtration), then add 10 times of water, simmer for 30 minutes, and filter the boiled liquid with 4 layers of gauze. Drug residue. Combine the two solutions and let sit overnight. The next day, take the supernatant, centrifuge the sediment (3000 rpm, 20 minutes), combine all the supernatants, boil and concentrate to 400 mL, then concentrate the supernatant in a 100°C water bath to 120 mL of water decoction, divide it into three parts, each 40 mL portion, add 80 mL 95% absolute ethanol to make it 63% ethanol; add 160 mL 95% absolute ethanol to make it 76% ethanol; add 240 mL 95% absolute ethanol to make it 81% ethanol, and leave it at room temperature overnight for 24 hours. , combine the three alcohol solubles and alcohol precipitates obtained to form an alcohol soluble product mixture and an alcohol precipitate mixture. The alcohol-soluble product mixture and the alcohol-precipitated product mixture were concentrated in a 100°C water bath to a paste, filtered under reduced pressure to form dry powder, dried in a drying oven at 80°C, and set aside.

Example 3: Analysis results of each component of the crude extracts of the four embodiments and three Chinese medicine combinations containing Smilax poria.

The implementation process of determining the total flavonoids content in the test solution:

Precisely weigh 2g of the dry powder of alcohol-soluble Chinese medicine and completely dissolve it in 20ml of water. Pour it into the polyamide resin column from top to bottom. Repeat elution with 95% ethanol 5 times. The eluent is the total flavonoids in the sample. Filter the eluate, concentrate, and adjust the volume to 25 ml. Pipette 1ml of the test solution, draw the following standard rutin curve, and measure the absorbance value.

Accurately weigh 2.5 mg of rutin reference substance, place it in a 25 ml volumetric flask, add methanol to dissolve, and shake well to obtain a standard solution with a concentration of 0.1 mg/ml. Take 0, 0.5, 1, 1.5, 2, 2.5, 3 mL of rutin standard solution into a 10 mL volumetric flask respectively, add 4 mL of 1% AlCl3 solution, and dissolve 95% ethanol to 10 mL, shake well, and let stand for 15 minutes. Measure the absorbance value at 405nm, draw the standard curve with the concentration of the standard as the abscissa, and calculate the regression equation.

The implementation process of determining the total terpene content in the test solution:

Precisely weigh 2g of the dry powder of alcohol-soluble Chinese medicine and completely dissolve it in 20ml of water. Pour it into the D101 macroporous resin column from top to bottom. Repeat elution with 95% ethanol 5 times. The eluate is the total flavonoids in the sample. Filter the eluate, concentrate, and adjust the volume to 25 ml. Densely absorb 0.2 mL of the test solution sample, process it according to the treatment method of oleanolic acid reference solution, and measure the absorbance.

Oleanolic acid standard curve: Accurately weigh 5 mg of oleanolic acid, dilute it with ethanol into a 25ml volumetric flask, and prepare an oleanolic acid standard solution with a concentration of 0.2mg/ml. Precisely pipette 0.1, 0.2, 0.3, 0.4, 0.5, 0.6mL of oleanolic acid standard solution into a graduated test tube, heat to evaporate the solvent, and add 0.4mL of newly prepared 5% vanillin-glacial acetic acid solution and perchloric acid Place 1.6 mL in a constant temperature water bath at 60°C for 20 minutes and then cool to room temperature. Add ethyl acetate to adjust the volume to 5 ml. Shake well. Measure the absorbance at 550 nm. Draw a standard curve with the solubility of the reference solution as the abscissa and the absorbance as the ordinate. Calculate Get the regression equation.

Table 1 Analysis results of components of the crude extract of Example 3

It can be seen from Table 1 that Scutellaria baicalensis, as a rhizome medicinal material, has a relatively high sugar content because it mainly contains flavonoids. Therefore, the increase in the flavonoid content of the four Chinese medicine combinations and the three Chinese medicine groups is greater than the total terpenoid content. increase. Flavonoids are one of the main chemical components responsible for antibacterial effects. The main component of Smilax Poria is not only aglycones or saponins, but also triterpenoids. It also contains flavonoids, phenols, organic acids and other substances. The chemical components of dandelion mainly include flavonoids, terpenes, fenacs, phytosterols, sesquiterpene lactones and coumarins. Honeysuckle mainly includes volatile oils, flavonoids, organic acids, iridoid glycosides, triterpene saponins and other components. These drugs with the same origin as medicine and food play a superimposed effect on total flavonoids and total terpenes. It has strong antibacterial and anti-inflammatory effects.

Example 4: Antibacterial effect of crude extracts of four traditional Chinese medicine combinations

The following experiments were taken for verification:

Different compositions of traditional Chinese medicine at 30g/mL were diluted with sterile water into solutions containing different concentrations of crude drugs such as 5%, 10%, 15%, 20%, and 30%. Use sterile water to prepare each activated test strain into a bacterial suspension containing approximately 1*10 ⁸ CFU/ml. Use the sterile filter paper disk method. The diameter of the paper disk prepared by the hole device is 6 mm, and the MH solid medium is used. The dosage of each disk is 5 microliters. Each concentration is repeated three times. Observe whether there is an inhibitory zone around the filter paper.

It can be seen from Table 2 that the decoction of Smilax poria, dandelion, honeysuckle, and skullcap has a strong inhibitory effect on Escherichia coli, Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, and Bacillus subtilis, and the higher the concentration, the The stronger the antibacterial activity, the weaker the inhibitory effect on Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.

Table 2 Antibacterial effects of four kinds of traditional Chinese medicine composition water decoctions at different concentrations (expressed by the diameter of the inhibitory zone, mm)

-No inhibition zone

Example 5: Protective effect of four traditional Chinese medicine combination extracts on mice with acute liver injury

The following experiments were conducted for verification: the model group was given 0.9% normal saline (10ml/kg) by gavage, and the positive group was given bifendate dropping pill suspension (150mg/kg) by gavage. The combination of four traditional Chinese medicines had high The medium and low dose groups were given corresponding doses of four traditional Chinese medicine combination solutions (crude medicine/mouse weight: 2g/kg, 1g/kg, 0.5g/kg) by gavage. After 10 days of administration, the subjects were weighed again 2 hours after the last administration. The injection site was the right abdominal cavity. Except for the blank group, all other groups were intraperitoneally injected with 10% carbon tetrachloride peanut oil mixture (10ml/kg) to successfully establish Acute liver injury model modeling.

Table 3 Example 5: The protective effects of different traditional Chinese medicine composition extracts on mice with acute liver injury

Group Liver index (g/10g) Thymus index (mg/g) Spleen index (mg/g) Weight(g) normal group 0.48±0.3 3.27±1.09 3.25±1.41 33.68±0.02 model group 0.59±0.4 4.41±0.03 5.15±0.91 29.68±0.04 positive control group O.48±02 3.15±0.05 3.57±0.05 31.68±0.01 Four kinds of traditional Chinese medicine high-dose group 0.43±0.02 3.37±0.26 3.35±1.01 33.68±0.06 Four Chinese medicine medium dose groups 0.49±0.04 3.76±0.29 3.85±1.11 32.68±0.08 Four kinds of traditional Chinese medicine low-dose group 0.50±0.01 4.06±0.29 4.25±0.91 30.68±0.03

It can be seen from Table 3 that the high- and medium-dose decoction groups of Smilax, dandelion, honeysuckle, and skullcap can reduce liver index, and the high-, medium-, and low-dose groups of Prunella vulgaris can reduce spleen lipid count and thymus coefficient. Traditional Chinese medicine has a detoxifying effect on injured mice, and the body has a certain degree of resistance without significant weight loss.

Although the present invention has been disclosed above in terms of preferred embodiments, they are not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, The protection scope of the present invention should be defined by the claims.

Claims (6)

1. A traditional Chinese medicine compound agent with antibacterial, anti-inflammatory, antiseptic and quality-guaranteeing effects is characterized in that traditional Chinese medicines of glabrous greenbrier rhizome, dandelion, honeysuckle and baical skullcap root are combined into a biological compound agent (powder or medicine bag), wherein the ratio of glabrous greenbrier rhizome, dandelion, honeysuckle (even branches and leaves) to baical skullcap root is 1:1:1:1; the rhizoma smilacis glabrae is dried rhizome of plants; the dandelion is dried whole herb of the plant; the honeysuckle is dried flower bud or flower with primary opening of honeysuckle plant; the radix Scutellariae is plant dried root.

2. A process for preparing the extract of claim 2, wherein the Chinese-medicinal composition of claim 1 is prepared by heating, decocting to obtain decoction, treating with alcohol gradient concentration overnight precipitation method to obtain mixed solution of different alcohol concentrations (alcohol solution for short) and mixed solution of different alcohol concentrations (alcohol precipitate for short), concentrating the filtrate at 100deg.C until it is steamed to paste, filtering under reduced pressure to obtain dry powder, drying at 80deg.C to obtain coarse powder of alcohol solution and coarse powder of alcohol precipitate, and purifying to obtain Chinese-medicinal powder or suspension.

3. The method of claim 2, wherein the pretreatment is soaking the herbal composition in water at room temperature for 2 hours, the amount of water added being 20 times the total amount of herbal medicine.

4. The method of claim 2, wherein the preparation of the decoction is carried out by boiling the pretreated Chinese medicinal composition, decocting with small fire for 30 min, collecting decoction, adding 10 times of water, boiling with small fire for 30 min, collecting decoction, mixing the two decoctions, standing overnight, collecting supernatant the next day, centrifuging the precipitate at 3000rpm for 20min, collecting supernatant, concentrating the supernatant to 400mL at 100deg.C, concentrating to 120mL with water bath at 100deg.C, and standing.

5. The method of claim 2, wherein the ethanol gradient overnight precipitation is performed by dividing 120mL of the decoction concentrate into three parts, adding 80mL of 95% absolute ethanol to each 40mL of the three parts to form 63% ethanol, adding 160mL of 95% absolute ethanol to 40mL of the decoction concentrate to form 76% ethanol, adding 240mL of 95% absolute ethanol to 100mL of the decoction concentrate to form 81% ethanol, and mixing the three obtained alcohol solutions and the alcohol sediment to form an alcohol solution mixture and an alcohol sediment mixture at room temperature for 24 hours.

6. The process of claim 2 wherein the alcohol solution mixture and the alcohol precipitate mixture are each concentrated to a paste by a water bath at 100 ℃, filtered under reduced pressure to form a dry powder, and dried in a dry oven at 80 ℃ to form a crude extract of the alcohol solution and the alcohol precipitate for use. And (5) separating and purifying the coarse powder to obtain traditional Chinese medicine powder or suspension preparation.