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CN117652648A - Chicken enzymolysis product, chicken powder condiment and preparation method - Google Patents

Chicken enzymolysis product, chicken powder condiment and preparation method Download PDF

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CN117652648A
CN117652648A CN202311664886.5A CN202311664886A CN117652648A CN 117652648 A CN117652648 A CN 117652648A CN 202311664886 A CN202311664886 A CN 202311664886A CN 117652648 A CN117652648 A CN 117652648A
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chicken
enzymatic
enzymatic hydrolysis
hydrolyzate
slurry
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祖庆勇
邢国辉
邓莉
丁红梅
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Xianzhiran Biotechnology Group Co ltd
Xianzhiran Guangdong Biotechnology Co ltd
Xianzhiran Tianjin Biotechnology Co ltd
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Xianzhiran Guangdong Biotechnology Co ltd
Xianzhiran Tianjin Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B4/00Preservation of meat, sausages, fish or fish products
    • A23B4/14Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
    • A23B4/18Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
    • A23B4/20Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/10Meat meal or powder; Granules, agglomerates or flakes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/48Addition of, or treatment with, enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/50Poultry products, e.g. poultry sausages
    • A23L13/52Comminuted, emulsified or processed products; Pastes; Reformed or compressed products from poultry meat
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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Abstract

本申请涉及一种鸡肉酶解物、鸡肉粉调味品、制备方法。一种鸡肉酶解物的制备方法,包括如下步骤:采用酸性蛋白酶和脂肪酶对鸡肉浆料进行第一酶解,制备第一酶解产物;采用碱性蛋白酶和减油酶对第一酶解产物进行第二酶解,制备鸡肉酶解物。本申请通过特定的酶解工艺将鸡肉浆料进行酶解,所得到的鸡肉酶解物中富含鸡肉抗氧化肽,将鸡肉酶解物中的抗氧化肽直接用于鸡肉粉调味品生产过程中,作为自源抗氧化剂,同样可以起到较好的抗氧化效果,延长产品的保质期,可减少如维生素E、迷迭香提取物等其他天然抗氧化剂的添加量,有利于节约原料成本。而且在后续制备鸡肉粉调味品的过程中,无需专门从鸡肉酶解物中分离出鸡肉抗氧化肽,减少了分离成本。The present application relates to a chicken enzymatic hydrolyzate, chicken powder condiment, and preparation method. A method for preparing enzymatic hydrolyzate of chicken, including the following steps: using acid protease and lipase to perform a first enzymatic hydrolysis of chicken slurry to prepare a first enzymatic hydrolyzate; using alkaline protease and oil-reducing enzyme to perform the first enzymatic hydrolysis The product undergoes a second enzymatic hydrolysis to prepare chicken enzymatic hydrolyzate. This application enzymatically hydrolyzes chicken slurry through a specific enzymatic hydrolysis process. The obtained chicken enzymatic hydrolyzate is rich in chicken antioxidant peptides. The antioxidant peptides in the chicken enzymatic hydrolyzate are directly used in the production process of chicken powder condiments. As a self-source antioxidant, it can also have a good antioxidant effect, extend the shelf life of the product, and reduce the amount of other natural antioxidants such as vitamin E and rosemary extract, which is beneficial to saving raw material costs. Moreover, in the subsequent preparation of chicken powder condiments, there is no need to specifically separate chicken antioxidant peptides from chicken enzymatic hydrolysates, reducing separation costs.

Description

鸡肉酶解物、鸡肉粉调味品、制备方法Chicken enzymatic hydrolyzate, chicken powder condiment, preparation method

技术领域Technical field

本申请涉及食品加工及调味品技术领域,特别是涉及一种鸡肉酶解物、鸡肉粉调味品、制备方法。The present application relates to the technical fields of food processing and condiments, and in particular to a chicken enzymatic hydrolyzate, chicken powder condiment, and preparation method.

背景技术Background technique

随着消费市场上以鸡肉粉调味品为代表的成品调味品的迅猛发展,消费者对产品的风味和品质提出了更高的要求,为适应于鸡肉粉调味品的品质升级,对鸡肉粉调味品的配料表和抗氧化剂的添加有了新的需求。With the rapid development of finished condiments represented by chicken powder condiments in the consumer market, consumers have put forward higher requirements for the flavor and quality of products. In order to adapt to the quality upgrade of chicken powder condiments, chicken powder condiments have There are new demands for the ingredient list of products and the addition of antioxidants.

目前市场上的鸡肉粉调味品采用高压蒸煮、粉碎、喷雾干燥得到,或者采用酶解、灭酶、喷雾干燥得到。上述方法制备得到的鸡肉粉调味品中含有的脂肪量在25-50%之间,脂肪含量高,脂肪氧化哈败现象特别严重。为解决脂肪氧化问题,传统技术中,通常采用化学抗氧化剂或天然抗氧化剂提升鸡肉粉调味品的抗氧化效果。化学抗氧化剂价格低,但是大众对化学抗氧化剂的认可度低,甚至排斥,因此应用较少。而对于天然抗氧化剂例如维生素E和迷迭香提取物,则更加容易被广大消费者所接受,但是这类天然抗氧化剂的价格高且所需添加量较多,例如,若需达到12个月的保质期,维生素E的添加量一般要达到3%,迷迭香提取物添加量一般要达到0.02%,从而导致鸡肉粉调味品的生产成本增加。The chicken powder condiments currently on the market are obtained by high-pressure cooking, crushing, and spray drying, or by enzymatic hydrolysis, enzyme inactivation, and spray drying. The fat content in the chicken powder seasoning prepared by the above method is between 25-50%, the fat content is high, and the fat oxidation failure phenomenon is particularly serious. In order to solve the problem of fat oxidation, in traditional technology, chemical antioxidants or natural antioxidants are usually used to enhance the antioxidant effect of chicken powder condiments. The price of chemical antioxidants is low, but the public’s recognition of chemical antioxidants is low or even rejection, so they are rarely used. Natural antioxidants such as vitamin E and rosemary extract are more easily accepted by consumers. However, these natural antioxidants are expensive and require a large amount of addition. For example, if it needs to be 12 months To ensure the shelf life, the added amount of vitamin E generally reaches 3%, and the added amount of rosemary extract generally reaches 0.02%, which leads to an increase in the production cost of chicken powder condiments.

鸡肉抗氧化肽是一种来源于鸡肉自身的生物活性肽,而且也属于天然来源的抗氧化肽,易吸收、营养价值更高,不会引起免疫反应,相比于比维生素E和迷迭香提取物等抗氧化剂还更加安全。如果可以将鸡肉抗氧化肽应用于鸡肉粉调味品的生产过程中,将有利于减少天然抗氧化剂的添加量。然而,目前仅有针对鸡肉抗氧化肽的产生和抗氧化性能的研究,并没有利用鸡肉抗氧化肽制备鸡肉粉调味品的成功案例。Chicken antioxidant peptide is a bioactive peptide derived from chicken itself, and it is also a naturally derived antioxidant peptide. It is easy to absorb, has higher nutritional value, and does not cause immune reactions. Compared with vitamin E and rosemary Antioxidants such as extracts are also safer. If chicken antioxidant peptides can be applied in the production process of chicken powder condiments, it will be beneficial to reduce the amount of natural antioxidants added. However, there are currently only studies on the production and antioxidant properties of chicken antioxidant peptides, and there are no successful cases of using chicken antioxidant peptides to prepare chicken powder condiments.

发明内容Contents of the invention

基于此,本申请提供了一种鸡肉酶解物的制备方法,利用所述方法制备的鸡肉酶解物可以直接利用酶解物自身的鸡肉抗氧化肽制备鸡肉粉调味品,从而可以减少天然抗氧化剂的添加。Based on this, this application provides a method for preparing chicken enzymatic hydrolyzate. The chicken enzymatic hydrolyzate prepared by the method can directly use the chicken antioxidant peptides of the enzymatic hydrolyzate itself to prepare chicken powder condiments, thereby reducing the natural antioxidant peptide. Addition of oxidizing agent.

进一步地,本申请还提供了一种鸡肉酶解物。Furthermore, this application also provides a chicken enzymatic hydrolyzate.

进一步地,本申请还提供了一种鸡肉粉调味品及其制备方法。Furthermore, this application also provides a chicken powder condiment and a preparation method thereof.

第一方面,本申请提供了一种鸡肉酶解物的制备方法,包括如下步骤:In a first aspect, this application provides a method for preparing chicken enzymatic hydrolyzate, including the following steps:

采用酸性蛋白酶和脂肪酶对鸡肉浆料进行第一酶解,制备第一酶解产物;以及Using acid protease and lipase to perform first enzymatic hydrolysis of chicken slurry to prepare a first enzymatic hydrolysis product; and

采用碱性蛋白酶和减油酶对所述第一酶解产物进行第二酶解,制备鸡肉酶解物。Alkaline protease and oil-reducing enzyme are used to perform a second enzymatic hydrolysis on the first enzymatic hydrolyzate to prepare a chicken enzymatic hydrolyzate.

在其中一些实施例中,所述鸡肉浆料采用包括如下步骤制备得到:In some embodiments, the chicken slurry is prepared by the following steps:

将鸡胸肉、鸡板油打碎、磨浆,制备鸡肉磨浆产物;以及Crush and grind chicken breast and chicken suet to prepare a chicken grinding product; and

将所述鸡肉磨浆产物与水混合,制备所述鸡肉浆料。The chicken meat slurry is prepared by mixing the chicken meat grinding product with water.

在其中一些实施例中,所述鸡胸肉和所述鸡板油的质量比为(9:1)~(9.5:0.5)。In some embodiments, the mass ratio of the chicken breast and the chicken suet is (9:1) ~ (9.5:0.5).

在其中一些实施例中,所述鸡肉磨浆产物与所述水的质量比为1:(0.25~3)。In some embodiments, the mass ratio of the chicken pulp product to the water is 1: (0.25~3).

在其中一些实施例中,所述酸性蛋白酶选自胃蛋白酶、木瓜蛋白酶、菠萝蛋白酶以及微生物蛋白酶AXH中的一种或多种。In some embodiments, the acidic protease is selected from one or more of pepsin, papain, bromelain and microbial protease AXH.

在其中一些实施例中,所述脂肪酶选自脂肪酶Lipozyme 435。In some of these embodiments, the lipase is selected from lipase Lipozyme 435.

在其中一些实施例中,所述酸性蛋白酶的添加量为所述鸡肉浆料的重量的0.05%~0.1%。In some embodiments, the acid protease is added in an amount of 0.05% to 0.1% of the weight of the chicken slurry.

在其中一些实施例中,所述脂肪酶的添加量为所述鸡肉浆料的重量的0.05%~0.1%。In some embodiments, the lipase is added in an amount of 0.05% to 0.1% of the weight of the chicken slurry.

在其中一些实施例中,第一酶解的酶解温度为50℃~60℃。In some embodiments, the enzymatic hydrolysis temperature of the first enzymatic hydrolysis is 50°C to 60°C.

在其中一些实施例中,第一酶解的酶解时间为0.5h~3h。In some embodiments, the enzymatic hydrolysis time of the first enzymatic hydrolysis is 0.5h~3h.

在其中一些实施例中,所述碱性蛋白酶选自碱性蛋白酶AXH。In some embodiments, the alkaline protease is selected from the group consisting of alkaline protease AXH.

在其中一些实施例中,所述减油酶选自减油酶Optiva LessFat BG。In some embodiments, the oil reducing enzyme is selected from the group consisting of Optiva LessFat BG.

在其中一些实施例中,所述碱性蛋白酶的添加量为所述鸡肉浆料的重量的0.05%~0.1%。In some embodiments, the alkaline protease is added in an amount of 0.05% to 0.1% of the weight of the chicken slurry.

在其中一些实施例中,所述减油酶的添加量为所述鸡肉浆料的重量的0.05%~0.1%。In some embodiments, the added amount of the oil-reducing enzyme is 0.05% to 0.1% of the weight of the chicken slurry.

在其中一些实施例中,在第二酶解前将所述第一酶解产物的pH值调节至7~9。In some embodiments, the pH value of the first enzymatic hydrolysis product is adjusted to 7 to 9 before the second enzymatic hydrolysis.

在其中一些实施例中,第二酶解的酶解温度为45℃~65℃。In some embodiments, the enzymatic hydrolysis temperature of the second enzymatic hydrolysis is 45°C to 65°C.

在其中一些实施例中,第二酶解的酶解时间为1h~4h。In some embodiments, the enzymatic hydrolysis time of the second enzymatic hydrolysis is 1 h to 4 h.

第二方面,本申请提供了一种鸡肉酶解物,由上述第一方面任一实施例中所述的制备方法制备得到。In a second aspect, the present application provides a chicken enzymatic hydrolyzate prepared by the preparation method described in any embodiment of the first aspect.

第三方面,本申请提供了一种鸡肉粉调味品,包括上述第二方面任一实施例中所述的鸡肉酶解物以及天然抗氧化剂。In a third aspect, this application provides a chicken powder condiment, including the chicken enzymatic hydrolyzate and natural antioxidants described in any embodiment of the second aspect.

在其中一些实施例中,所述天然抗氧化剂包括不超过所述鸡肉酶解物质量的1%的维生素E,或,包括不超过所述鸡肉酶解物质量的0.01%的迷迭香提取物。In some embodiments, the natural antioxidant includes vitamin E not exceeding 1% of the mass of the chicken enzymatic hydrolyzate, or includes rosemary extract not exceeding 0.01% of the mass of the chicken enzymatic hydrolyzate. .

在其中一些实施例中,所述天然抗氧化剂为维生素E,所述维生素E的添加量为所述鸡肉酶解物质量的0.5%~1%。In some embodiments, the natural antioxidant is vitamin E, and the amount of vitamin E added is 0.5% to 1% of the mass of the chicken enzymatic hydrolyzate.

在其中一些实施例中,所述天然抗氧化剂为迷迭香提取物,所述迷迭香提取物的添加量为所述鸡肉酶解物质量的0.005%~0.01%。In some embodiments, the natural antioxidant is rosemary extract, and the added amount of the rosemary extract is 0.005% to 0.01% of the mass of the chicken enzymatic hydrolyzate.

在其中一些实施例中,所述迷迭香提取物中,所述迷迭香的质量含量为25%~35%。In some embodiments, in the rosemary extract, the mass content of the rosemary is 25% to 35%.

第四方面,本申请提供了一种如上述第三方面任一实施例中所述的鸡肉粉调味品的制备方法,包括如下步骤:In a fourth aspect, the present application provides a method for preparing chicken powder condiment as described in any embodiment of the third aspect, including the following steps:

混合所述鸡肉酶解物和所述天然抗氧化剂,形成混合物,并对所述混合物进行均质处理。The chicken enzymatic hydrolyzate and the natural antioxidant are mixed to form a mixture, and the mixture is homogenized.

本申请提供了一种鸡肉酶解物的制备方法,通过特定的酶解工艺将鸡肉浆料进行酶解,所得到的鸡肉酶解物中富含鸡肉抗氧化肽,将鸡肉酶解物中的抗氧化肽直接用于鸡肉粉调味品生产过程中,作为自源抗氧化剂,同样可以起到较好的抗氧化效果,延长产品的保质期,可减少如维生素E、迷迭香提取物等其他天然抗氧化剂的添加量,有利于节约原料成本。而且在后续制备鸡肉粉调味品的过程中,无需专门从鸡肉酶解物中分离出鸡肉抗氧化肽,减少了分离成本。This application provides a method for preparing chicken enzymatic hydrolyzate. The chicken slurry is enzymatically hydrolyzed through a specific enzymatic hydrolysis process. The obtained chicken enzymatic hydrolyzate is rich in chicken antioxidant peptides. The chicken enzymatic hydrolyzate is Antioxidant peptides are directly used in the production process of chicken powder condiments. As self-sourced antioxidants, they can also have a good antioxidant effect, extend the shelf life of the product, and can reduce the consumption of other natural ingredients such as vitamin E and rosemary extract. The added amount of antioxidants is beneficial to saving raw material costs. Moreover, in the subsequent preparation of chicken powder condiments, there is no need to specifically separate chicken antioxidant peptides from chicken enzymatic hydrolysates, reducing separation costs.

具体实施方式Detailed ways

为了便于理解本申请,下面将结合实施例对本申请进行更全面的描述。但是,本申请可以以许多不同的形式来实现,并不限于本申请所描述的实施例。相反地,提供这些实施例的目的是使对本申请的公开内容的理解更加透彻全面。In order to facilitate understanding of the present application, the present application will be described more comprehensively below in conjunction with embodiments. However, the present application can be implemented in many different forms and is not limited to the embodiments described in this application. Rather, these embodiments are provided so that a thorough understanding of the disclosure of the present application will be provided.

除非另有定义,本申请所使用的所有的技术和科学术语与属于本申请的技术领域的技术人员通常理解的含义相同。本文中在本申请的说明书中所使用的术语只是为了描述具体的实施例的目的,不是旨在于限制本申请。本申请所使用的术语“和/或”包括一个或多个相关的所列项目的任意的和所有的组合。Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by a person skilled in the technical field of this application. The terminology used herein in the description of the application is for the purpose of describing specific embodiments only and is not intended to limit the application. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

本申请中,以开放式描述的技术特征中,包括所列举特征组成的封闭式技术方案,也包括包含所列举特征的开放式技术方案。In this application, the technical features described in open format include closed technical solutions composed of the listed features, and also include open technical solutions including the listed features.

第一方面,本申请提供了一种鸡肉酶解物的制备方法,包括如下步骤:In a first aspect, this application provides a method for preparing chicken enzymatic hydrolyzate, including the following steps:

采用酸性蛋白酶和脂肪酶对鸡肉浆料进行第一酶解,制备第一酶解产物;以及Using acid protease and lipase to perform first enzymatic hydrolysis of chicken slurry to prepare a first enzymatic hydrolysis product; and

采用碱性蛋白酶和减油酶对第一酶解产物进行第二酶解,制备鸡肉酶解物。Alkaline protease and oil-reducing enzyme are used to perform a second enzymatic hydrolysis on the first enzymatic hydrolyzate to prepare a chicken enzymatic hydrolyzate.

本申请提供了一种鸡肉酶解物的制备方法,通过特定的酶解工艺将鸡肉浆料进行酶解,所得到的鸡肉酶解物中富含鸡肉抗氧化肽,将鸡肉酶解物中的抗氧化肽直接用于鸡肉粉调味品生产过程中,作为自源抗氧化剂,同样可以起到较好的抗氧化效果,延长产品的保质期,可减少如维生素E、迷迭香提取物等其他天然抗氧化剂的添加量,有利于节约原料成本。而且在后续制备鸡肉粉调味品的过程中,无需专门从鸡肉酶解物中分离出鸡肉抗氧化肽,减少了分离成本。This application provides a method for preparing chicken enzymatic hydrolyzate. The chicken slurry is enzymatically hydrolyzed through a specific enzymatic hydrolysis process. The obtained chicken enzymatic hydrolyzate is rich in chicken antioxidant peptides. The chicken enzymatic hydrolyzate is Antioxidant peptides are directly used in the production process of chicken powder condiments. As self-sourced antioxidants, they can also have a good antioxidant effect, extend the shelf life of the product, and can reduce the consumption of other natural ingredients such as vitamin E and rosemary extract. The added amount of antioxidants is beneficial to saving raw material costs. Moreover, in the subsequent preparation of chicken powder condiments, there is no need to specifically separate chicken antioxidant peptides from chicken enzymatic hydrolysates, reducing separation costs.

进一步地,本申请在制备鸡肉酶解物的过程中,没有添加有机酸碱试剂,减少了化学原料对食品安全影响,而且还添加了脂肪酶和减油酶,使得产品不仅具有较长的保质期,还具有较为充盈的香气,减少了产品的油腻感,有利于增加食欲,且有利于降低产品的结团概率,改善了产品外观。Furthermore, in the process of preparing chicken enzymatic hydrolyzate, this application does not add organic acid-base reagents, which reduces the impact of chemical raw materials on food safety, and also adds lipase and oil-reducing enzyme, so that the product not only has a longer shelf life , also has a relatively full aroma, reduces the greasiness of the product, helps increase appetite, reduces the probability of product agglomeration, and improves the appearance of the product.

在其中一些实施例中,鸡肉浆料采用包括如下步骤制备得到:In some of the embodiments, the chicken slurry is prepared by the following steps:

将鸡胸肉、鸡板油打碎、磨浆,制备鸡肉磨浆产物;以及Crush and grind chicken breast and chicken suet to prepare a chicken grinding product; and

将鸡肉磨浆产物与水混合,制备鸡肉浆料。The chicken pulp product is mixed with water to prepare a chicken slurry.

鸡胸肉和鸡板油具有丰富的蛋白质和氨基酸,营养成分丰富,且脂肪含量适宜,使鸡肉粉调味品具有更加宜人的香气。Chicken breast and chicken suet are rich in protein and amino acids, rich in nutrients, and have appropriate fat content, giving the chicken powder condiment a more pleasant aroma.

在其中一些实施例中,鸡胸肉和鸡板油的质量比为(9:1)~(9.5:0.5)。鸡胸肉和鸡板油的质量比在上述范围内,可以使得制备得到的鸡肉粉调味品富含蛋白质等营养成分,而且脂肪含量足以产生足够的香气,同时又能避免脂肪含量过高使得鸡肉粉调味品结团。可以理解地,鸡胸肉和鸡肉粉调味品鸡板油的质量比例如可以但不限于为9:1、9.1:0.9、9.2:0.8、9.3:0.7、9.4:0.6、9.5:0.5等等。In some embodiments, the mass ratio of chicken breast to chicken suet is (9:1) ~ (9.5:0.5). The mass ratio of chicken breast to chicken suet is within the above range, so that the prepared chicken powder condiment is rich in protein and other nutrients, and the fat content is sufficient to produce sufficient aroma, while avoiding excessive fat content that makes the chicken powder seasoning Taste the group. It can be understood that the mass ratio of chicken breast and chicken powder condiment chicken suet can be, for example, but not limited to, 9:1, 9.1:0.9, 9.2:0.8, 9.3:0.7, 9.4:0.6, 9.5:0.5, etc.

在其中一些实施例中,鸡肉磨浆产物与水的质量比为1:(0.25~3)。向鸡肉磨浆产物中加入适宜的水,使得浆料具有较好的流动性,后续酶解过程中与酶的接触更加充分,提高酶解效果,同时避免了过高水量影响后续干燥过程。可以理解地,鸡肉磨浆产物与水的质量比例如可以但不限于为1:0.25、1:0.5、1:1、1:2、1:3等等。In some embodiments, the mass ratio of the chicken pulp product to water is 1: (0.25~3). Adding appropriate water to the chicken pulp product makes the slurry have better fluidity, and the subsequent enzymatic hydrolysis process will be more fully in contact with the enzyme, improving the enzymatic hydrolysis effect, while avoiding excessive water volume from affecting the subsequent drying process. It can be understood that the mass ratio of the chicken pulp product to water can be, for example, but not limited to, 1:0.25, 1:0.5, 1:1, 1:2, 1:3, etc.

在其中一些实施例中,酸性蛋白酶选自胃蛋白酶、木瓜蛋白酶、菠萝蛋白酶以及微生物蛋白酶AXH中的一种或多种。鸡肉为酸性物质,采用上述酸性蛋白酶进行酶解有利于将鸡肉中的蛋白质分解为小分子肽段或者氨基酸。In some embodiments, the acidic protease is selected from one or more of pepsin, papain, bromelain, and microbial protease AXH. Chicken is an acidic substance, and enzymatic hydrolysis using the above-mentioned acid protease is beneficial to decomposing the protein in chicken into small molecule peptides or amino acids.

进一步地,胃蛋白酶的酶活力例如可以但不限于为4000 U/g,木瓜蛋白酶的酶活力例如可以但不限于为60万u/g,菠萝蛋白酶的酶活力例如可以但不限于为50万u/g,微生物蛋白酶AXH的酶活力例如可以但不限于为 20万u/g。Further, the enzyme activity of pepsin can be, for example, but is not limited to, 4000 U/g, the enzyme activity of papain can be, for example, but is not limited to, 600,000 u/g, and the enzyme activity of bromelain can be, for example, but is not limited to, 500,000 u/g. /g, the enzyme activity of microbial protease AXH can be, for example, but is not limited to, 200,000 u/g.

在其中一些实施例中,脂肪酶选自脂肪酶Lipozyme 435。采用上述脂肪酶对鸡肉进行酶解,可以增加鸡肉粉调味品的香气。In some of these embodiments, the lipase is selected from Lipozyme 435. Using the above-mentioned lipase to enzymatically hydrolyze chicken can increase the aroma of chicken powder condiments.

在其中一些实施例中,酸性蛋白酶的添加量为鸡肉浆料的重量的0.05%~0.1%。可以理解地,酸性蛋白酶的添加量例如可以但不限于为鸡肉浆料的重量的0.05%、0.06%、0.07%、0.08%、0.09%、0.1%等等。In some embodiments, the acid protease is added in an amount of 0.05% to 0.1% of the weight of the chicken slurry. It can be understood that the amount of acidic protease added can be, for example, but is not limited to, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, etc. based on the weight of the chicken slurry.

在其中一些实施例中,脂肪酶的添加量为鸡肉浆料的重量的0.05%~0.1%。可以理解地,脂肪酶的添加量例如可以但不限于为鸡肉浆料的重量的0.05%、0.06%、0.07%、0.08%、0.09%、0.1%等等。In some embodiments, the lipase is added in an amount of 0.05% to 0.1% of the weight of the chicken slurry. It can be understood that the amount of lipase added can be, for example, but is not limited to, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, etc. based on the weight of the chicken slurry.

在其中一些实施例中,第一酶解的酶解温度为50℃~60℃。可以理解地,第一酶解的酶解温度例如可以但不限于为50℃、55℃、60℃等等。In some embodiments, the enzymatic hydrolysis temperature of the first enzymatic hydrolysis is 50°C to 60°C. It can be understood that the enzymatic hydrolysis temperature of the first enzymatic hydrolysis can be, for example, but not limited to, 50°C, 55°C, 60°C, etc.

在其中一些实施例中,第一酶解的酶解时间为0.5h~3h。可以理解地,第一酶解的酶解时间例如可以但不限于为0.5h、2h、3h等等。In some embodiments, the enzymatic hydrolysis time of the first enzymatic hydrolysis is 0.5h~3h. It can be understood that the enzymatic hydrolysis time of the first enzymatic hydrolysis can be, for example, but not limited to, 0.5h, 2h, 3h, etc.

在其中一些实施例中,碱性蛋白酶选自碱性蛋白酶AXH。在第一酶解后进一步采用碱性蛋白酶进行第二酶解,可以将鸡肉中的蛋白质进一步分解。进一步地,碱性蛋白酶AXH的酶活力例如可以但不限于为30万 U/g。In some of these embodiments, the alkaline protease is selected from alkaline protease AXH. After the first enzymatic hydrolysis, alkaline protease is further used for the second enzymatic hydrolysis, which can further decompose the protein in the chicken. Further, the enzyme activity of alkaline protease AXH can be, for example, but is not limited to, 300,000 U/g.

在其中一些实施例中,减油酶选自减油酶OptivaLessFatBG。在第二酶解的过程中,加入减油酶可以减少产品的结团概率,使得鸡肉粉更加松散。In some of these embodiments, the oil-reducing enzyme is selected from the group consisting of oil-reducing enzymes OptivaLessFatBG. In the second enzymatic hydrolysis process, adding oil-reducing enzyme can reduce the probability of product agglomeration and make the chicken meal looser.

在其中一些实施例中,碱性蛋白酶的添加量为鸡肉浆料的重量的0.05%~0.1%。可以理解地,碱性蛋白酶的添加量例如可以但不限于为鸡肉浆料的重量的0.05%、0.06%、0.07%、0.08%、0.09%、0.1%等等。In some embodiments, the amount of alkaline protease added is 0.05% to 0.1% of the weight of the chicken slurry. It can be understood that the amount of alkaline protease added can be, for example, but is not limited to, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, etc. based on the weight of the chicken slurry.

在其中一些实施例中,减油酶的添加量为鸡肉浆料的重量的0.05%~0.1%。可以理解地,减油酶的添加量例如可以但不限于为鸡肉浆料的重量的0.05%、0.06%、0.07%、0.08%、0.09%、0.1%等等。In some embodiments, the amount of oil-reducing enzyme added is 0.05% to 0.1% of the weight of the chicken slurry. It can be understood that the amount of oil-reducing enzyme added can be, for example, but is not limited to, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, etc. based on the weight of the chicken slurry.

在其中一些实施例中,在第二酶解前将第一酶解产物的pH值调节至7~9。在上述pH条件下,碱性蛋白酶和减油酶可以充分发挥酶解作用。可以理解地,在第二酶解前例如可以但不限于将第一酶解产物的pH值调节至7、8、9等等。In some embodiments, the pH value of the first enzymatic hydrolysis product is adjusted to 7 to 9 before the second enzymatic hydrolysis. Under the above pH conditions, alkaline protease and oil-reducing enzyme can fully exert their enzymatic effects. It is understood that before the second enzymatic hydrolysis, the pH value of the first enzymatic hydrolysis product may be adjusted to 7, 8, 9, etc., for example but is not limited to.

在其中一些实施例中,第二酶解的酶解温度为45℃~65℃。可以理解地,第二酶解的酶解温度例如可以但不限于为45℃、50℃、55℃、60℃、65℃等等。In some embodiments, the enzymatic hydrolysis temperature of the second enzymatic hydrolysis is 45°C to 65°C. It can be understood that the enzymatic hydrolysis temperature of the second enzymatic hydrolysis can be, for example, but not limited to, 45°C, 50°C, 55°C, 60°C, 65°C, etc.

在其中一些实施例中,第二酶解的酶解时间为1h~4h。可以理解地,第二酶解的酶解时间例如可以但不限于为1h、2h、3h、4h等等。In some embodiments, the enzymatic hydrolysis time of the second enzymatic hydrolysis is 1 h to 4 h. It can be understood that the enzymatic hydrolysis time of the second enzymatic hydrolysis can be, for example, but not limited to, 1 hour, 2 hours, 3 hours, 4 hours, etc.

在其中一些实施例中,在第二酶解结束后,还包括灭酶的步骤。In some of the embodiments, after the second enzymatic hydrolysis is completed, an enzyme inactivation step is also included.

进一步地,灭酶的条件包括:在110℃~130℃的温度下加热处理25min~35min。Further, the conditions for enzyme inactivation include: heat treatment at a temperature of 110°C to 130°C for 25min to 35min.

第二方面,本申请提供了一种鸡肉酶解物,由上述第一方面任一实施例中的制备方法制备得到。In a second aspect, this application provides a chicken enzymatic hydrolyzate prepared by the preparation method in any embodiment of the first aspect.

第三方面,本申请提供了一种鸡肉粉调味品,包括上述第二方面任一实施例中的鸡肉酶解物以及天然抗氧化剂。In a third aspect, this application provides a chicken powder condiment, including the chicken enzymatic hydrolyzate and natural antioxidants in any embodiment of the second aspect.

在其中一些实施例中,天然抗氧化剂包括不超过鸡肉酶解物质量的1%的维生素E,或,包括不超过鸡肉酶解物质量的0.01%的迷迭香提取物。在传统技术中,制备鸡肉粉调味品的过程中通常需要添加3%以上的维生素E或添加0.02%以上的迷迭香提取物才能使鸡肉粉调味品的保质期达到12个月以上,才能满足产品货架期的要求,而采用本申请的制备方法,由于酶解液中的鸡肉抗氧化肽即可起到较好的抗氧化效果,可减少其他天然抗氧化剂维生素E或迷迭香提取物的添加量即能达到相同保质期,大大节约了原料成本,有利于食品安全。In some embodiments, the natural antioxidant includes vitamin E not exceeding 1% of the mass of the chicken enzymatic hydrolyzate, or includes rosemary extract not exceeding 0.01% of the mass of the chicken enzymatic hydrolyzate. In traditional technology, in the process of preparing chicken powder condiment, it is usually necessary to add more than 3% vitamin E or add more than 0.02% rosemary extract to make the shelf life of chicken powder condiment reach more than 12 months to meet the product requirements. Shelf life requirements, and using the preparation method of this application, since the chicken antioxidant peptides in the enzymatic solution can have a better antioxidant effect, the addition of other natural antioxidants vitamin E or rosemary extract can be reduced The same shelf life can be achieved with the same quantity, which greatly saves the cost of raw materials and is conducive to food safety.

在其中一些实施例中,天然抗氧化剂为维生素E,维生素E的添加量为鸡肉酶解物质量的0.5%~1%。维生素E的添加量仅需0.5%即可使鸡肉粉调味品的保质期达到9月以上,添加量仅需1%即可使鸡肉粉调味品的保质期达到12月以上,满足产品货架期要求,可减少原料成本。可以理解地,维生素E的添加量例如可以但不限于为鸡肉酶解物质量的0.5%、0.8%、1%等等。In some embodiments, the natural antioxidant is vitamin E, and the amount of vitamin E added is 0.5% to 1% of the mass of the chicken enzymatic hydrolyzate. The added amount of vitamin E only needs 0.5% to make the shelf life of chicken powder condiments reach more than 9 months, and the added amount only needs 1% to make the shelf life of chicken powder condiments reach more than 12 months, meeting the product shelf life requirements. Reduce raw material costs. It can be understood that the amount of vitamin E added can be, for example, but is not limited to, 0.5%, 0.8%, 1%, etc. of the mass of the chicken enzymatic hydrolyzate.

在其中一些实施例中,天然抗氧化剂为迷迭香提取物,迷迭香提取物的添加量为鸡肉酶解物质量的0.005%~0.02%。迷迭香提取物的添加量仅需0.005%即可使鸡肉粉调味品的保质期达到9月以上,迷迭香提取物的添加量仅需0.01%即可使鸡肉粉调味品的保质期达到12月以上,满足产品货架期要求,可减少原料成本。可以理解地,迷迭香提取物的添加量例如可以但不限于为鸡肉酶解物质量的0.005%、0.01%等等。In some embodiments, the natural antioxidant is rosemary extract, and the amount of rosemary extract added is 0.005% to 0.02% of the mass of the chicken enzymatic hydrolyzate. The added amount of rosemary extract only needs 0.005% to make the shelf life of chicken powder condiment reach more than 9 months, and the added amount of rosemary extract only needs 0.01% to make the shelf life of chicken powder condiment reach 12 months. The above can meet the product shelf life requirements and reduce raw material costs. It can be understood that the added amount of rosemary extract can be, for example, but is not limited to, 0.005%, 0.01%, etc. of the mass of the chicken enzymatic hydrolyzate.

进一步地,迷迭香提取物中,迷迭香的质量含量为25%~35%。进一步地,迷迭香提取物中,迷迭香的质量含量为30%。Further, in the rosemary extract, the mass content of rosemary is 25% to 35%. Further, in the rosemary extract, the mass content of rosemary is 30%.

第四方面,本申请提供了一种如上述第三方面任一实施例中的鸡肉粉调味品的制备方法,包括如下步骤:In a fourth aspect, the present application provides a method for preparing chicken powder condiment as in any embodiment of the third aspect, including the following steps:

混合鸡肉酶解物和天然抗氧化剂,形成混合物,并对混合物进行均质处理。Mix chicken enzymatic hydrolyzate and natural antioxidants to form a mixture, and homogenize the mixture.

进一步地,均质处理的条件包括:均质压力为25MPa~35MPa。Further, the conditions for homogenization treatment include: the homogenization pressure is 25MPa~35MPa.

在其中一些实施例中,均质处理的步骤之后还包括依次进行干燥、粉碎和灭菌的步骤。In some embodiments, the step of homogenizing is followed by the steps of drying, crushing and sterilizing in sequence.

进一步地,干燥处理后物料中的水分的质量含量不超过5%。Further, the mass content of moisture in the material after drying treatment does not exceed 5%.

可选地,干燥处理的方式为喷雾干燥。Optionally, the drying process is spray drying.

可选地,喷雾干燥的进风温度为150℃~200℃、出口温度为80℃~120℃。Optionally, the inlet air temperature of spray drying is 150°C~200°C, and the outlet temperature is 80°C~120°C.

进一步地,粉碎处理后物料的粒径大小不超过100目。Further, the particle size of the crushed material shall not exceed 100 mesh.

以下通过具体实施例对本申请的鸡肉酶解物、鸡肉粉调味品、制备方法作进一步详细的说明。以下实施例较为具体,可以理解地,在其他实施例中,不限于此。在以下具体实施例中,所涉及的仪器、试剂、材料,若无特别说明,均为现有技术中已有的常规仪器、试剂、材料,可通过正规商业途径获得。下述实施例中所涉及的实验方法、检测方法等,若无特别说明,均为现有技术中已有的常规实验方法、检测方法。The chicken enzymatic hydrolyzate, chicken powder condiment, and preparation method of the present application will be further described in detail through specific examples. The following embodiments are more specific, and it is understood that in other embodiments, they are not limited thereto. In the following specific examples, the instruments, reagents, and materials involved, unless otherwise specified, are all conventional instruments, reagents, and materials existing in the prior art and can be obtained through regular commercial channels. The experimental methods, detection methods, etc. involved in the following examples are all conventional experimental methods and detection methods existing in the prior art unless otherwise specified.

酸性蛋白酶:木瓜蛋白酶,酶活力60万u/g;菠萝蛋白酶,酶活力50万u/g。Acid protease: papain, enzyme activity 600,000 u/g; bromelain, enzyme activity 500,000 u/g.

脂肪酶:脂肪酶Lipozyme 435,酶活力3万u/g。Lipase: Lipozyme 435, enzyme activity 30,000 u/g.

碱性蛋白酶:碱性蛋白酶AXH,酶活力30万u/g。Alkaline protease: Alkaline protease AXH, enzyme activity 300,000 u/g.

减油酶:减油酶Optiva LessFat BG,酶活力25万u/g。Oil-reducing enzyme: Oil-reducing enzyme Optiva LessFat BG, enzyme activity 250,000 u/g.

实施例1Example 1

一种鸡肉粉调味品的制备方法,包括如下步骤:A preparation method of chicken powder condiment, including the following steps:

步骤一:将鸡胸肉90Kg、鸡板油10Kg打碎、磨浆,制备鸡肉磨浆产物,将鸡肉磨浆产物与水按照质量比1:0.25混合,制备鸡肉浆料。Step 1: Crush and grind 90Kg of chicken breast and 10Kg of chicken suet to prepare a chicken pulp product. Mix the chicken pulp product and water according to a mass ratio of 1:0.25 to prepare a chicken slurry.

步骤二:向步骤一制备得到的鸡肉浆料中依次加入木瓜蛋白酶和脂肪酶对鸡肉浆料进行第一酶解,木瓜蛋白酶和脂肪酶的添加量分别为鸡肉浆料的重量的0.05%、0.05%,在温度为50℃的条件下酶解3h,制备第一酶解产物。Step 2: Add papain and lipase to the chicken slurry prepared in step 1 in sequence to perform the first enzymatic hydrolysis of the chicken slurry. The added amounts of papain and lipase are respectively 0.05% and 0.05% of the weight of the chicken slurry. %, enzymatically hydrolyzed for 3 hours at a temperature of 50°C to prepare the first enzymatically hydrolyzed product.

步骤三:将步骤二制备得到的第一酶解产物的pH值调节至7.5,然后向第一酶解产物中依次加入碱性蛋白酶和减油酶对第一酶解产物进行第二酶解,碱性蛋白酶和减油酶的添加量分别为鸡肉浆料的重量的0.05%、0.05%,在温度为60℃的条件下酶解1h,制备鸡肉酶解物,在120℃的温度下加热处理30min进行灭酶。Step 3: Adjust the pH value of the first enzymatic hydrolysis product prepared in step 2 to 7.5, and then add alkaline protease and oil-reducing enzyme to the first enzymatic hydrolysis product in sequence to perform a second enzymatic hydrolysis of the first enzymatic hydrolysis product. The addition amounts of alkaline protease and oil-reducing enzyme are 0.05% and 0.05% of the weight of the chicken slurry respectively. Enzymatic hydrolysis is performed at a temperature of 60°C for 1 hour to prepare the chicken enzymatic hydrolyzate, which is heated at a temperature of 120°C. Inactivate the enzyme for 30 minutes.

步骤四:将步骤三制备得到的鸡肉酶解物和维生素E混合,维生素E的添加量为鸡肉酶解物质量的1%,对混合物进行均质处理,制备均质处理产物,对均质处理产物依次进行干燥处理、粉碎处理、灭菌处理和包装,制备鸡肉粉调味品;Step 4: Mix the chicken enzymatic hydrolyzate prepared in step 3 with vitamin E. The amount of vitamin E added is 1% of the mass of the chicken enzymatic hydrolyzate. Homogenize the mixture to prepare a homogenized product. The products are dried, crushed, sterilized and packaged in sequence to prepare chicken powder condiments;

均质处理的条件包括:均质压力为35MPa。干燥处理后物料中的水分的质量含量不超过5%。粉碎处理后物料的粒径大小不超过100目。The conditions for homogenization treatment include: the homogenization pressure is 35MPa. The mass content of moisture in the material after drying treatment shall not exceed 5%. The particle size of the material after crushing treatment shall not exceed 100 mesh.

实施例2Example 2

一种鸡肉粉调味品的制备方法,包括如下步骤:A preparation method of chicken powder condiment, including the following steps:

步骤一:将鸡胸肉95Kg、鸡板油5Kg打碎、磨浆,制备鸡肉磨浆产物,将鸡肉磨浆产物与水按照质量比1:0.25混合,制备鸡肉浆料。Step 1: Crush and grind 95Kg of chicken breast and 5Kg of chicken suet to prepare a chicken grinding product. Mix the chicken grinding product and water according to a mass ratio of 1:0.25 to prepare a chicken slurry.

步骤二:向步骤一制备得到的鸡肉浆料中依次加入菠萝蛋白酶和脂肪酶对鸡肉浆料进行第一酶解,木瓜蛋白酶和脂肪酶的添加量分别为鸡肉浆料的重量的0.05%、0.05%,在温度为60℃的条件下酶解2h,制备第一酶解产物。Step 2: Add bromelain and lipase to the chicken slurry prepared in step 1 in sequence to perform the first enzymatic hydrolysis of the chicken slurry. The added amounts of papain and lipase are 0.05% and 0.05% of the weight of the chicken slurry respectively. %, enzymatically hydrolyzed for 2 hours at a temperature of 60°C to prepare the first enzymatically hydrolyzed product.

步骤三:将步骤二制备得到的第一酶解产物的pH值调节至8.5,然后向第一酶解产物中依次加入碱性蛋白酶和减油酶对第一酶解产物进行第二酶解,碱性蛋白酶和减油酶的添加量分别为鸡肉浆料的重量的0.05%、0.05%,在温度为60℃的条件下酶解4h,制备鸡肉酶解物,在120℃的温度下加热处理30min进行灭酶。Step 3: Adjust the pH value of the first enzymatic hydrolysis product prepared in step 2 to 8.5, and then add alkaline protease and oil-reducing enzyme to the first enzymatic hydrolysis product in sequence to perform a second enzymatic hydrolysis of the first enzymatic hydrolysis product. The addition amounts of alkaline protease and oil-reducing enzyme are 0.05% and 0.05% of the weight of the chicken slurry respectively. Enzymatic hydrolysis is performed at 60°C for 4 hours to prepare chicken enzymatic hydrolyzate, which is heated at 120°C. Inactivate the enzyme for 30 minutes.

步骤四:将步骤三制备得到的鸡肉酶解物和迷迭香的质量含量为30%的迷迭香提取物混合,迷迭香提取物的添加量为鸡肉酶解物质量的0.01%,对混合物进行均质处理,制备均质处理产物,对均质处理产物依次进行干燥处理、粉碎处理、灭菌处理和包装,制备鸡肉粉调味品;Step 4: Mix the chicken enzymatic hydrolyzate prepared in step 3 with a rosemary extract with a mass content of 30%. The added amount of rosemary extract is 0.01% of the mass of the chicken enzymatic hydrolyzate. The mixture is homogenized to prepare a homogenized product, and the homogenized product is sequentially dried, crushed, sterilized and packaged to prepare chicken powder condiments;

均质处理的条件包括:均质压力为25MPa。干燥处理后物料中的水分的质量含量不超过5%。粉碎处理后物料的粒径大小不超过100目。The conditions for homogenization treatment include: the homogenization pressure is 25MPa. The mass content of moisture in the material after drying treatment shall not exceed 5%. The particle size of the material after crushing treatment shall not exceed 100 mesh.

对比例1Comparative example 1

与实施例1大致相同,区别在于:对比例1没有进行步骤二的第一酶解和步骤三的第二酶解。It is roughly the same as Example 1, except that Comparative Example 1 did not perform the first enzymatic hydrolysis in step 2 and the second enzymatic hydrolysis in step three.

一种鸡肉粉调味品的制备方法,包括如下步骤:A preparation method of chicken powder condiment, including the following steps:

步骤一:与实施例1步骤一相同。Step 1: Same as step 1 in Example 1.

步骤二:将步骤一制备得到的鸡肉浆料和维生素E混合,维生素E的添加量为鸡肉浆料质量的1%,对混合物进行均质处理,制备均质处理产物,对均质处理产物依次进行干燥处理、粉碎处理、灭菌处理和包装,制备鸡肉粉调味品;Step 2: Mix the chicken slurry prepared in step 1 with vitamin E. The amount of vitamin E added is 1% of the mass of the chicken slurry. The mixture is homogenized to prepare a homogenized product. The homogenized product is processed in sequence. Carry out drying, crushing, sterilization and packaging to prepare chicken powder condiments;

均质处理的条件包括:均质压力为35MPa。干燥处理后物料中的水分的质量含量不超过5%。粉碎处理后物料的粒径大小不超过100目。The conditions for homogenization treatment include: the homogenization pressure is 35MPa. The mass content of moisture in the material after drying treatment shall not exceed 5%. The particle size of the material after crushing treatment shall not exceed 100 mesh.

对比例2Comparative example 2

与对比例1大致相同,区别在于,对比例2维生素E的添加量为鸡肉浆料质量的2%。It is roughly the same as Comparative Example 1, except that the amount of vitamin E added in Comparative Example 2 is 2% of the mass of the chicken slurry.

对比例3Comparative example 3

与对比例1大致相同,区别在于,对比例3维生素E的添加量为鸡肉浆料质量的3%。It is roughly the same as Comparative Example 1, except that the amount of vitamin E added in Comparative Example 3 is 3% of the mass of the chicken slurry.

对比例4Comparative example 4

与实施例2大致相同,区别在于,对比例4没有进行步骤二的第一酶解和步骤三的第二酶解,且将迷迭香提取物的添加量调整为0.015%。It is roughly the same as Example 2. The difference is that Comparative Example 4 does not perform the first enzymatic hydrolysis of step 2 and the second enzymatic hydrolysis of step 3, and the addition amount of rosemary extract is adjusted to 0.015%.

一种鸡肉粉调味品的制备方法,包括如下步骤:A preparation method of chicken powder condiment, including the following steps:

步骤一:与实施例2步骤一相同。Step 1: Same as step 1 in Example 2.

步骤二:将步骤一制备得到的鸡肉浆料和迷迭香的质量含量为30%的迷迭香提取物混合,迷迭香提取物的添加量为鸡肉浆料质量的0.015%,对混合物进行均质处理,制备均质处理产物,对均质处理产物依次进行干燥处理、粉碎处理、灭菌处理和包装,制备鸡肉粉调味品;Step 2: Mix the chicken slurry prepared in step 1 with a rosemary extract with a mass content of 30%. The added amount of rosemary extract is 0.015% of the mass of the chicken slurry. The mixture is Homogenizing treatment, preparing a homogenized product, drying, crushing, sterilizing and packaging the homogenized product in sequence to prepare chicken powder condiment;

均质处理的条件包括:均质压力为25MPa。干燥处理后物料中的水分的质量含量不超过5%。粉碎处理后物料的粒径大小不超过100目。The conditions for homogenization treatment include: the homogenization pressure is 25MPa. The mass content of moisture in the material after drying treatment shall not exceed 5%. The particle size of the material after crushing treatment shall not exceed 100 mesh.

对比例5Comparative example 5

与对比例4大致相同,区别在于,对比例5迷迭香提取物的添加量为鸡肉浆料质量的0.02%。It is roughly the same as Comparative Example 4, except that the added amount of rosemary extract in Comparative Example 5 is 0.02% of the mass of the chicken slurry.

对比例6Comparative example 6

与实施例1大致相同,区别在于,没有进行步骤二的第一酶解和步骤三的第二酶解,且未添加抗氧化剂。It is roughly the same as Example 1, except that the first enzymatic hydrolysis in step 2 and the second enzymatic hydrolysis in step 3 are not performed, and no antioxidant is added.

一种鸡肉粉调味品的制备方法,包括如下步骤:A preparation method of chicken powder condiment, including the following steps:

步骤一:与实施例1步骤一相同。Step 1: Same as step 1 in Example 1.

步骤二:将步骤一制备得到的鸡肉浆料进行均质处理,制备均质处理产物,对均质处理产物依次进行干燥处理、粉碎处理、灭菌处理和包装,制备鸡肉粉调味品;Step 2: homogenize the chicken slurry prepared in step 1 to prepare a homogenized product, and dry, crush, sterilize and package the homogenized product in sequence to prepare chicken powder condiment;

均质处理的条件包括:均质压力为35MPa。干燥处理后物料中的水分的质量含量不超过5%。粉碎处理后物料的粒径大小不超过100目。The conditions for homogenization treatment include: the homogenization pressure is 35MPa. The mass content of moisture in the material after drying treatment shall not exceed 5%. The particle size of the material after crushing treatment shall not exceed 100 mesh.

对比例7Comparative example 7

与实施例1大致相同,区别在于,在步骤二中未添加脂肪酶,在步骤三中未添加减油酶。It is roughly the same as Example 1, except that no lipase is added in step two and no oil-reducing enzyme is added in step three.

对实施例1~实施例2和对比例1~对比例7制备得到的鸡肉粉调味品进行评价,评价结果如下表1。The chicken powder condiments prepared in Examples 1 to 2 and Comparative Examples 1 to 7 were evaluated. The evaluation results are as follows in Table 1.

表1 鸡肉粉调味品的品质比较Table 1 Quality comparison of chicken powder condiments

以上所述实施例的各技术特征可以进行任意的组合,为使描述简洁,未对上述实施例中的各个技术特征所有可能的组合都进行描述,然而,只要这些技术特征的组合不存在矛盾,都应当认为是本说明书记载的范围。The technical features of the above embodiments can be combined in any way. To simplify the description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, All should be considered to be within the scope of this manual.

以上所述实施例仅表达了本发明的几种实施方式,其描述较为具体和详细,但并不能因此而理解为对发明专利范围的限制。应当指出的是,对于本领域的普通技术人员来说,在不脱离本发明构思的前提下,还可以做出若干变形和改进,这些都属于本申请的保护范围。因此,本申请专利的保护范围应以所附权利要求为准。The above-mentioned embodiments only express several implementation modes of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention. It should be noted that, for those of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these all fall within the protection scope of the present application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims (10)

1.一种鸡肉酶解物的制备方法,其特征在于,包括如下步骤:1. A method for preparing chicken enzymatic hydrolyzate, which is characterized by comprising the following steps: 采用酸性蛋白酶和脂肪酶对鸡肉浆料进行第一酶解,制备第一酶解产物;以及Using acid protease and lipase to perform first enzymatic hydrolysis of chicken slurry to prepare a first enzymatic hydrolysis product; and 采用碱性蛋白酶和减油酶对所述第一酶解产物进行第二酶解,制备鸡肉酶解物。Alkaline protease and oil-reducing enzyme are used to perform a second enzymatic hydrolysis on the first enzymatic hydrolyzate to prepare a chicken enzymatic hydrolyzate. 2.根据权利要求1所述的鸡肉酶解物的制备方法,其特征在于,所述鸡肉浆料采用包括如下步骤制备得到:2. The preparation method of chicken enzymatic hydrolyzate according to claim 1, characterized in that the chicken slurry is prepared by the following steps: 将鸡胸肉、鸡板油打碎、磨浆,制备鸡肉磨浆产物;以及Crush and grind chicken breast and chicken suet to prepare a chicken grinding product; and 将所述鸡肉磨浆产物与水混合,制备所述鸡肉浆料。The chicken meat slurry is prepared by mixing the chicken meat grinding product with water. 3.根据权利要求2所述的鸡肉酶解物的制备方法,其特征在于,所述鸡肉浆料的制备过程中,满足以下条件中的至少一个条件:3. The preparation method of chicken enzymatic hydrolyzate according to claim 2, characterized in that, during the preparation process of the chicken slurry, at least one of the following conditions is met: (1)所述鸡胸肉和所述鸡板油的质量比为(9:1)~(9.5:0.5);以及(1) The mass ratio of the chicken breast to the chicken suet is (9:1)~(9.5:0.5); and (2)所述鸡肉磨浆产物与所述水的质量比为1:(0.25~3)。(2) The mass ratio of the chicken pulp product to the water is 1: (0.25~3). 4.根据权利要求1所述的鸡肉酶解物的制备方法,其特征在于,第一酶解的条件包括以下条件中的至少一个条件:4. The preparation method of chicken enzymatic hydrolyzate according to claim 1, characterized in that the conditions for the first enzymatic hydrolysis include at least one of the following conditions: 1)所述酸性蛋白酶选自胃蛋白酶、木瓜蛋白酶、菠萝蛋白酶以及微生物蛋白酶AXH中的一种或多种;1) The acidic protease is selected from one or more types of pepsin, papain, bromelain and microbial protease AXH; 2)所述脂肪酶选自脂肪酶Lipozyme 435;2) The lipase is selected from lipase Lipozyme 435; 3)所述酸性蛋白酶的添加量为所述鸡肉浆料的重量的0.05%~0.1%;3) The addition amount of the acid protease is 0.05%~0.1% of the weight of the chicken slurry; 4)所述脂肪酶的添加量为所述鸡肉浆料的重量的0.05%~0.1%;4) The added amount of lipase is 0.05%~0.1% of the weight of the chicken slurry; 5)第一酶解的酶解温度为50℃~60℃;以及5) The enzymatic hydrolysis temperature of the first enzymatic hydrolysis is 50℃~60℃; and 6)第一酶解的酶解时间为0.5h~3h。6) The enzymatic hydrolysis time of the first enzymatic hydrolysis is 0.5h~3h. 5.根据权利要求1所述的鸡肉酶解物的制备方法,其特征在于,第二酶解的条件包括以下条件中的至少一个条件:5. The preparation method of chicken enzymatic hydrolyzate according to claim 1, characterized in that the conditions for the second enzymatic hydrolysis include at least one of the following conditions: (A)所述碱性蛋白酶选自碱性蛋白酶AXH;(A) The alkaline protease is selected from alkaline protease AXH; (B)所述减油酶选自减油酶Optiva LessFat BG;(B) The oil-reducing enzyme is selected from the group consisting of Optiva LessFat BG; (C)所述碱性蛋白酶的添加量为所述鸡肉浆料的重量的0.05%~0.1%;(C) The amount of alkaline protease added is 0.05% to 0.1% of the weight of the chicken slurry; (D)所述减油酶的添加量为所述鸡肉浆料的重量的0.05%~0.1%;(D) The added amount of the oil-reducing enzyme is 0.05% to 0.1% of the weight of the chicken slurry; (E)在第二酶解前将所述第一酶解产物的pH值调节至7~9;(E) Adjust the pH value of the first enzymatic hydrolysis product to 7 to 9 before the second enzymatic hydrolysis; (F)第二酶解的酶解温度为45℃~65℃;以及(F) The enzymatic hydrolysis temperature of the second enzymatic hydrolysis is 45℃~65℃; and (G)第二酶解的酶解时间为1h~4h。(G) The enzymatic hydrolysis time of the second enzymatic hydrolysis is 1h~4h. 6.一种鸡肉酶解物,其特征在于,由权利要求1~5任一项所述的制备方法制备得到。6. A chicken enzymatic hydrolyzate, characterized in that it is prepared by the preparation method described in any one of claims 1 to 5. 7.一种鸡肉粉调味品,其特征在于,包括权利要求6所述的鸡肉酶解物以及天然抗氧化剂。7. A chicken powder condiment, characterized by comprising the chicken enzymatic hydrolyzate of claim 6 and natural antioxidants. 8.根据权利要求7所述的鸡肉粉调味品,其特征在于,所述天然抗氧化剂包括不超过所述鸡肉酶解物质量的1%的维生素E,或,包括不超过所述鸡肉酶解物质量的0.01%的迷迭香提取物。8. The chicken powder condiment according to claim 7, characterized in that the natural antioxidant includes no more than 1% of the amount of vitamin E of the enzymatic hydrolyzate of the chicken, or includes no more than the enzymatic hydrolyzate of the chicken. 0.01% of rosemary extract by mass. 9.根据权利要求8所述的鸡肉粉调味品,其特征在于,所述天然抗氧化剂为维生素E,所述维生素E的添加量为所述鸡肉酶解物质量的0.5%~1%;或9. The chicken powder condiment according to claim 8, wherein the natural antioxidant is vitamin E, and the added amount of vitamin E is 0.5% to 1% of the mass of the chicken enzymatic hydrolyzate; or 所述天然抗氧化剂为迷迭香提取物,所述迷迭香提取物的添加量为所述鸡肉酶解物质量的0.005%~0.01%;可选地,所述迷迭香提取物中,所述迷迭香的质量含量为25%~35%。The natural antioxidant is rosemary extract, and the added amount of the rosemary extract is 0.005% to 0.01% of the mass of the chicken enzymatic hydrolyzate; optionally, in the rosemary extract, The mass content of the rosemary is 25%~35%. 10.一种如权利要求7~9任一项所述的鸡肉粉调味品的制备方法,其特征在于,包括如下步骤:10. A method for preparing chicken powder condiment as claimed in any one of claims 7 to 9, characterized in that it includes the following steps: 混合所述鸡肉酶解物和所述天然抗氧化剂,形成混合物,并对所述混合物进行均质处理。The chicken enzymatic hydrolyzate and the natural antioxidant are mixed to form a mixture, and the mixture is homogenized.
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CN108095018A (en) * 2017-10-10 2018-06-01 河北滦平华都食品有限公司 Chicken meat powder flavouring and preparation method thereof
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