CN117617459B - Fresh-keeping method for processing freshwater fish - Google Patents
Fresh-keeping method for processing freshwater fish Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
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- A23B4/00—Preservation of meat, sausages, fish or fish products
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- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
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- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
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- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
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- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
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Abstract
本发明涉及水产加工技术领域,尤其是一种淡水鱼的加工保鲜方法;包括:活鱼击晕的步骤、清除鱼体体表粘液的步骤、清除鱼体体表腥味层的步骤、鱼体施加海鱼粘液的步骤和包装的步骤;本发明的有益效果在于:先清除淡水鱼自身携带的具有腥臭味物质的粘液,而后在不破坏鱼体整体性的情况下将淡水鱼体表被腥臭味物质渗入的部分组织连同附着在这些组织表面的放线菌和蓝绿藻一同清除,最后在鱼体体表施加海鱼粘液,继而得到利于保鲜、腥臭味较淡且粘液不会随保鲜时间增加而增加腥臭味的淡水鱼整鱼产品。The invention relates to the technical field of aquatic product processing, in particular to a method for processing and preserving freshwater fish; the method comprises the steps of stunning a live fish, removing mucus from the surface of the fish, removing a fishy odor layer from the surface of the fish, applying marine fish mucus to the fish, and packaging. The method has the beneficial effects of first removing mucus with fishy odor substances carried by the freshwater fish itself, then removing part of the tissues on the surface of the freshwater fish infiltrated by the fishy odor substances together with actinomycetes and blue-green algae attached to the surfaces of the tissues without destroying the integrity of the fish body, and finally applying marine fish mucus to the surface of the fish body, thereby obtaining a whole freshwater fish product which is conducive to preservation, has a lighter fishy odor, and the mucus does not increase the fishy odor as the preservation time increases.
Description
技术领域Technical Field
本发明涉及水产加工技术领域,尤其是一种淡水鱼的加工保鲜方法。The invention relates to the technical field of aquatic product processing, in particular to a method for processing and preserving freshwater fish.
背景技术Background Art
近年来人们发现鱼类的体表粘液中含有抗氧化和抗菌物质(可减缓鱼皮和鱼肉的腐败),并将鱼类的体表粘液涂覆在需要保鲜的鱼体外表以达到保鲜目的,但目前仅限于海鱼冰鲜保鲜以及鱼肉制品(肉片、肉糜)保鲜。现有技术中鱼类体表粘液不适用于淡水鱼的保鲜,其主要原因是大部分淡水鱼本身粘液分泌较多,涂覆的粘液被鱼体本身粘液阻隔无法接触鱼体,不能产生实质性影响;淡水鱼腥臭味的主要来源之一就是粘液,涂覆更多的粘液意味着可能产生更大的腥臭味,淡水鱼死后其粘液中的腥臭物质会继续增加并容易渗入鱼皮和鱼肉,严重影响食用价值。In recent years, people have found that fish surface mucus contains antioxidant and antibacterial substances (which can slow down the corruption of fish skin and fish meat), and the fish surface mucus is applied to the surface of the fish body that needs to be preserved to achieve the purpose of preservation, but it is currently limited to the preservation of marine fish and fish products (meat slices, minced meat). In the prior art, fish surface mucus is not suitable for the preservation of freshwater fish. The main reason is that most freshwater fish secrete more mucus, and the applied mucus is blocked by the mucus of the fish body and cannot contact the fish body, and cannot produce a substantial effect; one of the main sources of the fishy smell of freshwater fish is mucus. Applying more mucus means that a greater fishy smell may be produced. After the death of freshwater fish, the fishy substances in its mucus will continue to increase and easily penetrate into the fish skin and fish meat, seriously affecting the edible value.
发明内容Summary of the invention
针对现有技术的不足,本发明提供一种淡水鱼的加工保鲜方法,该方法采用鱼类体表粘液作为整鱼保鲜剂且不会增加鱼体的腥臭味。In view of the deficiencies of the prior art, the present invention provides a method for processing and preserving freshwater fish, which uses mucus on the surface of fish as a whole fish preservative and does not increase the fishy smell of the fish body.
本发明的技术方案为:The technical solution of the present invention is:
一种淡水鱼的加工保鲜方法,包括:A method for processing and preserving freshwater fish, comprising:
活鱼击晕的步骤;和,Procedure for stunning live fish; and,
清除鱼体体表粘液的步骤;和,Steps for removing mucus from the fish body surface; and,
清除鱼体体表腥味层的步骤;和,The step of removing the fishy layer on the surface of the fish body; and,
鱼体施加海鱼粘液的步骤;和,The step of applying marine fish mucus to the fish body; and,
包装的步骤。Packaging steps.
优选地,所述活鱼击晕的方法为:以600~700V、0.5~1.5A的脉冲电击活鱼,脉冲频率为0.5~2.0Hz,电击时长1~3s。一般市面售卖的淡水鱼体重为2~5市斤,上述参数的电击可保证大部分市面售卖的淡水鱼快速晕死(击晕后窒息死亡),避免挣扎刮损鱼体。Preferably, the method of stunning live fish is: shocking live fish with pulses of 600-700V, 0.5-1.5A, with a pulse frequency of 0.5-2.0Hz and a shock duration of 1-3s. Generally, the weight of freshwater fish sold on the market is 2-5 kg. The above parameters of shock can ensure that most freshwater fish sold on the market will be stunned quickly (suffocated to death after stunning), avoiding struggling and scratching the fish body.
优选地,所述清除鱼体体表粘液的方法为:使鱼体体表附着木屑,刮除木屑,再次粘附木屑,再次刮除木屑,循环该操作直至鱼体体表无肉眼可见粘液,冲洗掉鱼体体表木屑,沥水备用。Preferably, the method for removing mucus from the fish body surface is: allowing sawdust to adhere to the fish body surface, scraping off the sawdust, allowing sawdust to adhere again, scraping off the sawdust again, repeating this operation until there is no mucus visible to the naked eye on the fish body surface, rinsing off the sawdust on the fish body surface, and draining for later use.
优选地,所述木屑为白千层(桃金娘科白千层属乔木)锯末。白千层木质柔软蓬松,不容易刮损鱼皮,另外白千层含有除臭成分(桉叶油醇、柠檬烯、β-石竹烯和α-蒎烯),反复摩擦鱼皮、鱼鳞可减轻鱼体腥臭味。Preferably, the sawdust is Melaleuca alternifolia (Myrtaceae Melaleuca alternifolia) sawdust. Melaleuca alternifolia wood is soft and fluffy, and is not easy to scratch the fish skin. In addition, Melaleuca alternifolia contains deodorizing ingredients (eucalyptol, limonene, β-caryophyllene and α-pinene), and repeated rubbing of fish skin and fish scales can reduce the fishy smell of the fish body.
优选地,所述清除鱼体体表腥味层的方法为:将嫩肉粉与水按照重量比1:80~90混合均匀得到去腥液,将鱼体浸泡在去腥液中3~7min,将鱼体取出后立刻转入75wt%乙醇溶液中浸泡2~5min,将鱼体取出后清水中浸泡2~5min,沥水备用。去腥液将鱼体外表的鱼鳞、鱼鳍、鱼尾、鱼头、鱼鳃、口腔、食道前端和部分裸露的鱼皮的外层水解,其原理是利用嫩肉粉对弹性蛋白、胶原蛋白具有强烈的水解作用,而对肌肉蛋白的水解作用较弱的特性,令其快速水解鱼体外表部分组织,同时也使得部分放线菌和蓝绿藻脱离,继而得到较为轻味的鱼体。而后通过乙醇使得嫩肉粉中的酶变性,继而终止去腥液继续水解鱼体,并顺带减少鱼体外表细菌数量。Preferably, the method for removing the fishy smell layer on the surface of the fish body is: the tender meat powder and water are mixed uniformly according to the weight ratio of 1:80-90 to obtain a deodorizing liquid, the fish body is soaked in the deodorizing liquid for 3-7 minutes, the fish body is taken out and immediately transferred to a 75wt% ethanol solution for 2-5 minutes, the fish body is taken out and soaked in clean water for 2-5 minutes, and the water is drained for standby use. The deodorizing liquid hydrolyzes the outer layer of the fish scales, fins, tails, heads, gills, mouths, front ends of the esophagus and partially exposed fish skin on the surface of the fish body. The principle is to use the tender meat powder to have a strong hydrolysis effect on elastin and collagen, but a weak hydrolysis effect on muscle protein, so that it can quickly hydrolyze some tissues on the surface of the fish body, and also make some actinomycetes and blue-green algae separate, and then obtain a fish body with a lighter taste. Then, the enzyme in the tender meat powder is denatured by ethanol, and then the deodorizing liquid is stopped to continue to hydrolyze the fish body, and the number of bacteria on the surface of the fish body is reduced.
优选地,所述去腥液温度控制在30~44℃,理论上嫩肉粉最佳水解温度为50~60℃,但鱼肉在高于45℃后容易发生蛋白质高温变性,故选择水解速度稍慢但不容易发生鱼肉蛋白质高温变性的温度。Preferably, the temperature of the deodorizing liquid is controlled at 30-44°C. Theoretically, the optimal hydrolysis temperature of tenderizer is 50-60°C, but fish protein is prone to high-temperature denaturation at temperatures above 45°C. Therefore, a temperature is selected that is slightly slower in hydrolysis but less prone to high-temperature denaturation of fish protein.
优选地,所述鱼体施加海鱼粘液的方法为:将新鲜大黄鱼体表刮下来的海鱼粘液与食品消泡剂、甘油按照重量比90:1~3:3~4搅拌得到混合液,在搅拌中将鱼体浸入混合液中,搅拌超过5min后开始抽真空,直至搅拌结束。海鱼粘液是大黄鱼加工中的废弃物;食品消泡剂用于防止混合液搅拌时产生过多的气泡,并利于抽真空时鱼体的鱼鳃、口腔和食道等处的空气脱离鱼体;甘油可提升海鱼粘液的保水能力,避免在抽真空时损失过多的水分。Preferably, the method of applying marine fish mucus to the fish body is: the marine fish mucus scraped from the surface of fresh large yellow croaker is stirred with a food defoamer and glycerin in a weight ratio of 90:1-3:3-4 to obtain a mixed solution, the fish body is immersed in the mixed solution during stirring, and vacuuming is started after stirring for more than 5 minutes until the stirring is completed. Marine fish mucus is a waste product in large yellow croaker processing; the food defoamer is used to prevent excessive bubbles from being generated when the mixed solution is stirred, and facilitates the air in the gills, oral cavity, esophagus, etc. of the fish body to be separated from the fish body when vacuuming; glycerin can enhance the water retention capacity of marine fish mucus and avoid excessive water loss during vacuuming.
优选地,所述搅拌速率为30~50r/min,搅拌时长8~11min,采用低速搅拌配合消泡剂,避免海鱼粘液产生大量微小气泡,也利于鱼体的鱼鳃、口腔和食道的空气排出;抽真空的真空度为-0.05~-0.10bar,采用较小的真空度,旨在诱导鱼鳃、口腔和食道的空气排出,减缓上述部位在后续保鲜过程中的氧化作用,又不至于造成鱼体内脏或粘膜破损。Preferably, the stirring rate is 30 to 50 r/min, the stirring time is 8 to 11 min, and low-speed stirring is used in combination with a defoaming agent to avoid the generation of a large number of tiny bubbles in the marine fish mucus, which is also beneficial to the discharge of air from the fish gills, mouth and esophagus of the fish body; the vacuum degree of vacuum extraction is -0.05 to -0.10 bar, and a smaller vacuum degree is used to induce the discharge of air from the fish gills, mouth and esophagus, slowing down the oxidation of the above-mentioned parts in the subsequent preservation process without causing damage to the fish's internal organs or mucous membranes.
优选地,所述食品消泡剂为DSA-5消泡剂。该消泡剂为食品级消泡剂,可用于食品加工。Preferably, the food defoamer is DSA-5 defoamer, which is a food grade defoamer and can be used in food processing.
优选地,所述包装的方法为:将鱼体从混合液中捞出放入包装袋内,将包装袋抽真空后密封袋口。本产品适合冷冻和冷藏保存。Preferably, the packaging method is: fish out the fish from the mixed liquid and put it into a packaging bag, and then vacuumize the packaging bag and seal the bag opening. This product is suitable for freezing and refrigeration.
本发明原理:Principle of the invention:
海鱼的腥臭味来源于鱼体死亡后体内肌肉分解产生的三甲胺,故新鲜大黄鱼加工产生的海鱼粘液其腥臭味较淡,并且因为脱离了鱼体,粘液不会随着时间推移增加腥臭味。而淡水鱼腥臭味来源于鱼体体表粘液中以及附着在鱼鳃、鱼鳞、皮肤、鱼鳍、鱼尾、口腔和食道表面的放线菌和蓝绿藻。淡水鱼粘液本身富含水分和营养,适合放线菌和蓝绿藻生存。鱼体死亡后鱼皮和肌肉分解并将营养释放到粘液中,为放线菌和蓝绿藻提供更加充足的养分,故淡水鱼的粘液在鱼体死亡后的一段时间内会因为放线菌和蓝绿藻快速增殖而增加腥臭味。并且事实证明鱼体体表粘液的抗菌物质对放线菌和蓝绿藻并没有有效抑制作用。本发明通过先清除鱼体体表粘液以及清除鱼体体表的鱼鳃、鱼鳞、皮肤、鱼鳍、鱼尾、口腔和食道处的外层组织,而后再将海鱼粘液施加在几乎不含有放线菌和蓝绿藻的鱼体体表,继而得到保鲜时间较长且腥臭味较少的淡水鱼整鱼产品。The fishy smell of marine fish comes from trimethylamine produced by muscle decomposition after the fish dies. Therefore, the fishy smell of marine fish mucus produced by processing fresh yellow croaker is lighter, and because it is separated from the fish body, the mucus will not increase in fishy smell over time. The fishy smell of freshwater fish comes from the actinomycetes and cyanobacteria in the mucus on the surface of the fish body and attached to the gills, scales, skin, fins, tail, mouth and esophagus. Freshwater fish mucus itself is rich in water and nutrients, which is suitable for the survival of actinomycetes and cyanobacteria. After the death of the fish, the skin and muscles decompose and release nutrients into the mucus, providing more sufficient nutrients for actinomycetes and cyanobacteria. Therefore, the mucus of freshwater fish will increase in fishy smell due to the rapid proliferation of actinomycetes and cyanobacteria for a period of time after the death of the fish. And it has been proven that the antibacterial substances in the mucus on the surface of the fish body have no effective inhibitory effect on actinomycetes and cyanobacteria. The invention removes mucus from the fish body surface and the outer tissues of the fish gills, fish scales, skin, fish fins, fish tail, oral cavity and esophagus on the fish body surface first, and then applies the marine fish mucus to the fish body surface which contains almost no actinomycetes and blue-green algae, thereby obtaining a whole freshwater fish product with a long shelf life and less fishy smell.
本发明的有益效果为:先清除淡水鱼自身携带的具有腥臭味物质的粘液,而后在不破坏鱼体整体性的情况下将淡水鱼体表被腥臭味物质渗入的部分组织连同附着在这些组织表面的放线菌和蓝绿藻一同清除,最后在鱼体体表施加海鱼粘液,继而得到利于保鲜、腥臭味较淡且粘液不会随保鲜时间增加而增加腥臭味的淡水鱼整鱼产品;本方法环保健康,不会在鱼体残留有毒物质,并且简单易操作,适合淡水鱼的批量加工。The beneficial effects of the present invention are as follows: firstly, the mucus with fishy smell substances carried by the freshwater fish itself is removed, and then, without destroying the integrity of the fish body, part of the tissues on the surface of the freshwater fish body that are infiltrated by the fishy smell substances together with the actinomycetes and blue-green algae attached to the surface of these tissues are removed, and finally, marine fish mucus is applied to the surface of the fish body, thereby obtaining a whole freshwater fish product that is conducive to preservation, has a lighter fishy smell, and the mucus will not increase the fishy smell as the preservation time increases; the method is environmentally friendly and healthy, will not leave toxic substances in the fish body, is simple and easy to operate, and is suitable for batch processing of freshwater fish.
具体实施方式DETAILED DESCRIPTION
下面结合对比例对本发明的具体实施方式作进一步说明:The specific embodiments of the present invention are further described below in conjunction with comparative examples:
实施例1Example 1
S1将淡水鱼放入水槽,用电鱼机以620V、0.6A的脉冲电击活鱼,脉冲频率为0.8Hz,电击时长1.2s,得到晕死的鱼体。实施例1-3以及对比例1-4所述淡水鱼均采用2市斤±10%的鲤鱼(鲤形目鲤科鲤属)。S1: Put freshwater fish into a water tank, and use an electric fish machine to shock the live fish with pulses of 620V and 0.6A, with a pulse frequency of 0.8Hz and a shock duration of 1.2s, to obtain a dead fish body. The freshwater fish described in Examples 1-3 and Comparative Examples 1-4 are all carp (Cypriniformes, Cyprinidae, Cyprinus) weighing 2 jin ± 10%.
S2使鱼体体表附着木屑,刮除木屑,再次粘附木屑,再次刮除木屑,循环该操作直至鱼体体表无肉眼可见粘液,冲洗掉鱼体体表木屑,沥水备用,所述木屑为白千层锯末。S2 makes sawdust adhere to the fish body surface, scrapes off the sawdust, makes sawdust adhere again, scrapes off the sawdust again, repeats this operation until there is no mucus visible to the naked eye on the fish body surface, rinses off the sawdust on the fish body surface, drains and sets aside, the sawdust is Melaleuca sawdust.
S3将嫩肉粉与水按照重量比1:82混合均匀得到去腥液,将鱼体浸泡在去腥液中4min,所述去腥液温度控制在31℃,搅动鱼体使其与去腥液充分接触,将鱼体取出后立刻转入75wt%乙醇溶液中浸泡2.5min,搅动鱼体使其与乙醇溶液充分接触,将鱼体取出后清水中浸泡2.5min,沥水备用。S3: Evenly mix the tenderizing powder and water in a weight ratio of 1:82 to obtain a deodorizing liquid, soak the fish body in the deodorizing liquid for 4 minutes, the temperature of the deodorizing liquid is controlled at 31°C, stir the fish body to make it fully contact with the deodorizing liquid, take out the fish body and immediately transfer it to a 75wt% ethanol solution and soak it for 2.5 minutes, stir the fish body to make it fully contact with the ethanol solution, take out the fish body and soak it in clean water for 2.5 minutes, drain and set aside.
S4将新鲜大黄鱼体表刮下来的海鱼粘液与食品消泡剂、甘油按照重量比90:1.2:3.2搅拌得到混合液,所述食品消泡剂为DSA-5消泡剂,在搅拌中将鱼体浸入混合液中,所述搅拌速率为32r/min,搅拌时长8.5min,搅拌超过5min后开始抽真空,抽真空的真空度为-0.07bar,直至搅拌结束。S4: The marine fish mucus scraped from the surface of fresh yellow croaker is stirred with a food defoamer and glycerin in a weight ratio of 90:1.2:3.2 to obtain a mixed liquid, wherein the food defoamer is DSA-5 defoamer. The fish body is immersed in the mixed liquid during stirring. The stirring rate is 32 r/min, and the stirring time is 8.5 min. After stirring for more than 5 min, vacuuming is started, and the vacuum degree of vacuuming is -0.07 bar, until the stirring is completed.
S5将鱼体从混合液中捞出放入包装袋内,将包装袋抽真空后密封袋口。S5 takes the fish body out of the mixed liquid and puts it into a packaging bag, and then evacuates the packaging bag and seals the bag opening.
实施例2Example 2
S1将淡水鱼放入水槽,用电鱼机以680V、1.4A的脉冲电击活鱼,脉冲频率为1.8Hz,电击时长2.5s,得到晕死的鱼体。S1 puts freshwater fish into a water tank and uses an electric fish machine to shock the live fish with pulses of 680V and 1.4A. The pulse frequency is 1.8Hz and the shock duration is 2.5s, resulting in a stunned fish body.
S2使鱼体体表附着木屑,刮除木屑,再次粘附木屑,再次刮除木屑,循环该操作直至鱼体体表无肉眼可见粘液,冲洗掉鱼体体表木屑,沥水备用,所述木屑为白千层锯末。S2 makes sawdust adhere to the fish body surface, scrapes off the sawdust, makes sawdust adhere again, scrapes off the sawdust again, repeats this operation until there is no mucus visible to the naked eye on the fish body surface, rinses off the sawdust on the fish body surface, drains and sets aside, the sawdust is Melaleuca sawdust.
S3将嫩肉粉与水按照重量比1:88混合均匀得到去腥液,将鱼体浸泡在去腥液中6min,所述去腥液温度控制在42℃,搅动鱼体使其与去腥液充分接触,将鱼体取出后立刻转入75wt%乙醇溶液中浸泡4min,搅动鱼体使其与乙醇溶液充分接触,将鱼体取出后清水中浸泡4min,沥水备用。S3: Evenly mix the tenderizing powder and water in a weight ratio of 1:88 to obtain a deodorizing liquid, soak the fish body in the deodorizing liquid for 6 minutes, the temperature of the deodorizing liquid is controlled at 42°C, stir the fish body to make it fully contact with the deodorizing liquid, take out the fish body and immediately transfer it to a 75wt% ethanol solution and soak it for 4 minutes, stir the fish body to make it fully contact with the ethanol solution, take out the fish body and soak it in clean water for 4 minutes, drain and set aside.
S4将新鲜大黄鱼体表刮下来的海鱼粘液与食品消泡剂、甘油按照重量比90:2.8:3.8搅拌得到混合液,所述食品消泡剂为DSA-5消泡剂,在搅拌中将鱼体浸入混合液中,所述搅拌速率为48r/min,搅拌时长10min,搅拌超过5min后开始抽真空,抽真空的真空度为-0.09bar,直至搅拌结束。S4: The marine fish mucus scraped from the surface of fresh large yellow croaker is stirred with a food defoamer and glycerin in a weight ratio of 90:2.8:3.8 to obtain a mixed liquid, wherein the food defoamer is DSA-5 defoamer. The fish body is immersed in the mixed liquid during stirring. The stirring rate is 48 r/min, and the stirring time is 10 min. After stirring for more than 5 min, vacuuming is started, and the vacuum degree of vacuuming is -0.09 bar, until the stirring is completed.
S5将鱼体从混合液中捞出放入包装袋内,将包装袋抽真空后密封袋口。S5 takes the fish body out of the mixed liquid and puts it into a packaging bag, and then evacuates the packaging bag and seals the bag opening.
实施例3Example 3
S1将淡水鱼放入水槽,用电鱼机以650V、1.0A的脉冲电击活鱼,脉冲频率为1.5Hz,电击时长1.5s,得到晕死的鱼体。S1 puts freshwater fish into a water tank, and uses an electric fish machine to shock the live fish with pulses of 650V and 1.0A. The pulse frequency is 1.5Hz and the shock duration is 1.5s, so that the fish is stunned.
S2使鱼体体表附着木屑,刮除木屑,再次粘附木屑,再次刮除木屑,循环该操作直至鱼体体表无肉眼可见粘液,冲洗掉鱼体体表木屑,沥水备用,所述木屑为白千层锯末。S2 makes sawdust adhere to the fish body surface, scrapes off the sawdust, makes sawdust adhere again, scrapes off the sawdust again, repeats this operation until there is no mucus visible to the naked eye on the fish body surface, rinses off the sawdust on the fish body surface, drains and sets aside, the sawdust is Melaleuca sawdust.
S3将嫩肉粉与水按照重量比1:85混合均匀得到去腥液,将鱼体浸泡在去腥液中5min,所述去腥液温度控制在39℃,搅动鱼体使其与去腥液充分接触,将鱼体取出后立刻转入75wt%乙醇溶液中浸泡3min,搅动鱼体使其与乙醇溶液充分接触,将鱼体取出后清水中浸泡3min,沥水备用。S3: Evenly mix the tenderizing powder and water in a weight ratio of 1:85 to obtain a deodorizing liquid, soak the fish body in the deodorizing liquid for 5 minutes, the temperature of the deodorizing liquid is controlled at 39°C, stir the fish body to make it fully contact with the deodorizing liquid, take out the fish body and immediately transfer it to a 75wt% ethanol solution and soak it for 3 minutes, stir the fish body to make it fully contact with the ethanol solution, take out the fish body and soak it in clean water for 3 minutes, drain and set aside.
S4将新鲜大黄鱼体表刮下来的海鱼粘液与食品消泡剂、甘油按照重量比90:1.5:3.5搅拌得到混合液,所述食品消泡剂为DSA-5消泡剂,在搅拌中将鱼体浸入混合液中,所述搅拌速率为40r/min,搅拌时长10min,搅拌超过5min后开始抽真空,抽真空的真空度为-0.07bar,直至搅拌结束。S4: The marine fish mucus scraped from the surface of fresh large yellow croaker is stirred with a food defoamer and glycerin in a weight ratio of 90:1.5:3.5 to obtain a mixed liquid, wherein the food defoamer is DSA-5 defoamer. The fish body is immersed in the mixed liquid during stirring. The stirring rate is 40 r/min, and the stirring time is 10 min. After stirring for more than 5 min, vacuuming is started, and the vacuum degree of vacuuming is -0.07 bar, until the stirring is completed.
S5将鱼体从混合液中捞出放入包装袋内,将包装袋抽真空后密封袋口。S5 takes the fish body out of the mixed liquid and puts it into a packaging bag, and then evacuates the packaging bag and seals the bag opening.
对比例1Comparative Example 1
取消步骤S2,其他步骤与实施例3相同。Step S2 is cancelled, and the other steps are the same as those in Example 3.
对比例2Comparative Example 2
将嫩肉粉用去腥粉替换,去腥粉为八角、胡椒和肉桂粉末按照重量比5:4:3混合均匀得到的粉末,其他步骤与实施例3相同。The meat tenderizer is replaced with deodorizing powder, which is a powder obtained by uniformly mixing star anise, pepper and cinnamon powder in a weight ratio of 5:4:3. The other steps are the same as those in Example 3.
对比例3Comparative Example 3
将海鱼粘液用生鱼(鲈形目鳢科鳢属)体表刮下来的粘液替换,其他步骤与实施例3相同。The marine fish mucus was replaced with mucus scraped from the body surface of raw fish (Channa genus, Channa family, Perciformes), and the other steps were the same as those in Example 3.
对比例4Comparative Example 4
将海鱼粘液用草鱼(鲤形目鲤科草鱼属)体表刮下来的粘液替换,其他步骤与实施例3相同。The marine fish mucus was replaced with mucus scraped from the body surface of grass carp (Cypriniformes, Cyprinidae, genus Ctenopharyngodon), and the other steps were the same as those in Example 3.
腥臭味实验Fishy smell experiment
实验目的,检验本发明得到的产品经过一定时间的冷藏后是否会增加腥臭味。The purpose of the experiment is to test whether the product obtained by the present invention will increase the fishy smell after being refrigerated for a certain period of time.
实验方法,9名实验人员独立实验,每人各取实施例1-3和对比例1-4刚制得的产品1件,开袋闻气味,按照腥臭味浓烈程度从轻到重标记为1-5分,标记后再次封袋放入冰箱4℃冷藏5天,而后取出开袋闻气味,按照腥臭味浓烈程度从轻到重标记为1-5分;将9名实验人员对各产品冷藏前后腥臭味的打分叠加得到积分进行对比并记录。Experimental method: 9 experimenters conducted independent experiments. Each experimenter took one freshly prepared product of Examples 1-3 and Comparative Examples 1-4, opened the bag and smelled it, and marked it with 1-5 points according to the intensity of the fishy smell from light to heavy. After marking, the bag was sealed again and placed in a refrigerator at 4°C for 5 days. Then the product was taken out, opened the bag and smelled it, and marked it with 1-5 points according to the intensity of the fishy smell from light to heavy. The scores of the 9 experimenters on the fishy smell of each product before and after refrigeration were added together to obtain the scores for comparison and recording.
实验结果,见表1。The experimental results are shown in Table 1.
表1各产品冷藏前后腥臭味积分记录表Table 1 Record of fishy odor scores of each product before and after refrigeration
实施例1-3得到的产品味轻,并且经过5天的冷藏后也没大幅增加腥臭味。对比例1、3和4由于体表有淡水鱼粘液,故其产品本身味道会稍重,经过5天的冷藏后淡水鱼粘液中的放线菌大量繁殖,导致腥臭味愈加浓烈。对比例2由于采用了去腥粉去腥,故产品味轻,但由于它体表含淡水鱼粘液,经过5天的冷藏后可产生较浓的腥臭味。The products obtained in Examples 1-3 had a light taste, and the fishy smell did not increase significantly after 5 days of cold storage. Comparative Examples 1, 3 and 4 had a slightly stronger taste because of the presence of freshwater fish mucus on the surface. After 5 days of cold storage, the actinomycetes in the freshwater fish mucus multiplied in large quantities, causing the fishy smell to become stronger. Comparative Example 2 had a light taste because of the use of deodorizing powder to remove the fishy smell, but because it contained freshwater fish mucus on the surface, it produced a strong fishy smell after 5 days of cold storage.
保鲜效果实验Fresh-keeping effect experiment
实验目的,验证本发明得到的产品具有保鲜功能。The purpose of the experiment is to verify that the product obtained by the present invention has a preservation function.
实验方法,对实施例1-3以及对比例1-4得到的产品4℃冷藏5天后进行挥发性盐基氮的检测;国家标准规定TVB-N值:一级鲜肉<15mg/100g;二级鲜肉15-25mg/100g;腐败肉>25mg/100g。Experimental method: the volatile basic nitrogen of the products obtained in Examples 1-3 and Comparative Examples 1-4 was tested after being refrigerated at 4°C for 5 days; the national standard stipulates the TVB-N value: first-grade fresh meat <15mg/100g; second-grade fresh meat 15-25mg/100g; spoiled meat >25mg/100g.
实验结果,见表2。The experimental results are shown in Table 2.
表2各产品冷藏后挥发性盐基氮检测TVB-N值记录表Table 2 TVB-N value record of volatile basic nitrogen test of each product after refrigeration
公知的,假单胞菌属、莫拉氏菌、不动杆菌属、黄杆菌属和希瓦氏菌属等细菌是导致低温贮藏鱼类腐败的主要微生物,结合表1和2可知,腥臭味的浓烈程度与腐败速度并没有直接关联。实施例1-3以及对比例2由于施加了大黄鱼体表刮得的海鱼粘液,故具有较优的抑菌性能(针对上述各类腐败细菌);对比例3施加了生鱼体表刮得的粘液,也具有较优的抑菌性能;而对比例1体表保留了鲤鱼体表粘液,即使施加大黄鱼体表刮得的海鱼粘液也难以保护鱼体;对比例4涂覆了草鱼体表刮得的粘液,无法抑制腐败,对比例1和4的实验发现并非所有鱼类的粘液均具备抑制腐败达到保鲜效果的特性。It is well known that bacteria such as Pseudomonas, Moraxella, Acinetobacter, Flavobacterium and Shewanella are the main microorganisms that cause the corruption of low-temperature stored fish. Combining Tables 1 and 2, it can be seen that the intensity of the fishy smell is not directly related to the corruption rate. Examples 1-3 and Comparative Example 2 have better antibacterial properties (for the above-mentioned types of corruption bacteria) because they apply the marine fish mucus scraped from the surface of large yellow croaker; Comparative Example 3 applies the mucus scraped from the surface of raw fish, which also has better antibacterial properties; while Comparative Example 1 retains the mucus on the surface of carp, and it is difficult to protect the fish body even if the marine fish mucus scraped from the surface of large yellow croaker is applied; Comparative Example 4 is coated with the mucus scraped from the surface of grass carp, which cannot inhibit corruption. The experiments of Comparative Examples 1 and 4 found that not all fish mucus have the characteristics of inhibiting corruption to achieve the preservation effect.
上述实施例和说明书中描述的只是说明本发明的原理和最佳实施例,在不脱离本发明精神和范围的前提下,本发明还会有各种变化和改进,这些变化和改进都落入要求保护的本发明范围内。The above embodiments and descriptions are only for illustrating the principles and best embodiments of the present invention. Without departing from the spirit and scope of the present invention, the present invention may be subject to various changes and improvements, all of which fall within the scope of the present invention to be protected.
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