CN117617381A

CN117617381A - Rumen bypass preparation and preparation method and application thereof

Info

Publication number: CN117617381A
Application number: CN202410044823.8A
Authority: CN
Inventors: 刘香; 李建东; 李鹤琼; 张其磊; 王晓东; 岳精栓; 耿倩倩; 梁小琼
Original assignee: Xinchang Xinhecheng Vitamin Co ltd; Zhejiang Weierxin Animal Nutrition And Health Products Co ltd; Zhejiang NHU Co Ltd
Current assignee: Xinchang Xinhecheng Vitamin Co ltd; Zhejiang Weierxin Animal Nutrition And Health Products Co ltd; Zhejiang NHU Co Ltd
Priority date: 2024-01-12
Filing date: 2024-01-12
Publication date: 2024-03-01

Abstract

The application relates to a rumen bypass preparation, and a preparation method and application thereof. The preparation method of the rumen bypass preparation comprises the following steps: emulsifying the nutrients with grease, an emulsifying agent and water to prepare an oil-in-water emulsifying system; granulating by using the oil-in-water emulsion system to prepare a core; emulsifying the nutrients with oil, an emulsifying agent and water to prepare a water-in-oil emulsifying system; coating the surface of the core with the water-in-oil emulsifying system to prepare a first coating; and melting the grease, and coating the melted grease on the surface of the first coating to prepare a second coating. The rumen bypass preparation can effectively pass through rumen and can be fully released in small intestine to be absorbed and utilized by animals.

Description

Rumen bypass preparation and preparation method and application thereof

Technical Field

The application relates to the technical field of animal daily ration, in particular to a rumen bypass preparation and a preparation method and application thereof.

Background

In recent years, the dairy cow breeding industry develops rapidly, and large-scale pastures gradually replace modes such as small pastures, breeding companies and the like. Many problems in the dairy cow breeding process are important factors affecting pasture benefits. For example, after calving, the energy maintenance requirement and the production requirement are increased due to the changes of physiology, diet, environment, management and the like, the dry matter feed intake is low, and a large amount of body fat is needed to provide energy and raw materials for body metabolic reaction and milk fat synthesis, so that the dairy cows are in an energy negative balance state, thereby causing ketosis, subclinical ketosis or fatty liver disease. Whether the dairy cow passes the perinatal period successfully is a great challenge facing the dairy cow producer farm, whether the dairy cow passes the perinatal period successfully or not determines the production performance of the dairy cow, and the key of reducing the accumulation of liver fat and accelerating the delivery of the dairy cow to the liver is the successful transition of the perinatal period. Glucose, choline or niacin are properly added in the production of cows, which is a nutritional solution for controlling ketosis of cows.

Glucose is an essential nutrient in animal life and production activities, is taken as an energy source and a carbon source to directly participate in the metabolism process in the organism, and is used for properly supplementing exogenous glucose in early lactation period, increasing the amount of absorbable glucose in small intestine and improving the blood sugar concentration, thus being an effective strategy for preventing and treating postpartum glucose metabolism imbalance of dairy cows, reducing disease occurrence and improving milk yield. Choline is an important component of very low density lipoprotein, and is also a methyl donor of carnitine in the fatty acid oxidation process, and the addition of choline can accelerate the oxidation of liver fatty acid and transport the fatty acid out of the liver, so that the fatty acid is prevented from being deposited in the liver. The addition of choline saves methionine for providing methyl groups (methionine is the most limiting amino acid in cows) and allows more methionine to be used in milk production. . Nicotinic acid, also called niacin, is a water-soluble B-group vitamin which is converted into Nicotinamide (NAM) with biological activity after entering the animal body, and is used as a component of Nicotinamide Adenine Dinucleotide (NAD) and Nicotinamide Adenine Dinucleotide Phosphate (NADP), and can promote the oxidation energy supply of fatty acid and ketone body, reduce the accumulation of ketone body, promote gluconeogenesis, improve glucose supply and relieve the postpartum energy negative balance when the organism oxidizes and breathes. A great deal of research at home and abroad shows that methionine and lysine are the first or second limiting amino acids when the lactating cows synthesize milk proteins. The rumen protected amino acid can provide metabolizable amino acid for small intestine for milk cows, and the metabolizable amino acid proportion of the small intestine is adjusted through different addition amounts, so that the balance state of the amino acid is improved, the utilization rate of feed protein is improved, the utilization of free amino acid by mammary tissue is promoted, the urea nitrogen content in milk and blood is reduced, the milk protein content and milk yield of the milk cows are improved, the daily ration protein level and the feed cost are reduced, and the reproductive performance of cows is improved.

However, due to the special digestive physiology of dairy cows, nutrients such as glucose, choline, nicotinic acid, methionine and lysine can be degraded and utilized by microorganisms in rumen of dairy cows, and cannot effectively reach small intestine to be absorbed. Therefore, it is desirable to protect these nutrients from rumen microorganisms by physical or chemical means (i.e., using rumen protection techniques), safely pass through the rumen, reach the abomasum and the small intestine, and be released again.

Traditional techniques for producing rumen bypass products mainly include 2 types:

the first is to mix the nutrients in the coating material in a molten state to form a mixture, spray the mixture through a nozzle having a plurality of small holes at a sufficient air pressure, spray the mixture from a temperature environment above the melting point into an air stream below the melting point, and solidify to form microspheres comprising the core and the coating material. For example, glucose and pH sensitive substances are added into melted saturated fatty acid and stirred uniformly, then the mixture is sprayed into a fluidized bed with cold air through a high-pressure spray head to obtain rumen bypass glucose particles, or glucose and pH sensitive substances are dispersed into melted solid vegetable oil, spherical particles are obtained after atomization and solidification by a centrifugal atomizer, and an anti-caking agent is mixed to obtain the rumen bypass nutrient preparation product.

The main problems of the first type of method are: the water-soluble nutrients are not compatible with the grease, so that the content of the nutrients in the product is uneven, and in order to enable the grease to disperse the nutrients and exert a certain protection effect, the content of the nutrients in the product is usually low, and in addition, the rumen bypass effect is also poor.

The second is to mix nutrient powder, binder and other excipients, add a small amount of water to make a wet material, subject to the procedures of granulating, shot blasting, drying, sieving, etc. to form uniform granules containing nutrients, the outer coating is realized by the conventional fluidized bed technique, the granules are free to flow from each other, the coating material is atomized into fine droplets which contact and cover the surface when the granules move, and the film layer of the molten coating material is crystallized at a temperature lower than the melting point of the coating material, forming microcapsules. For example, glucose and high temperature resistant lipase are added with water according to a certain proportion to form wet materials, then the wet materials are extruded and shot-blasted by an extruder, the pellets are dried to obtain glucose pellets, then the pellets are placed into a fluidized bed to fluidize, finally molten fatty alcohols or saturated fatty acids containing pH sensitive substances are sprayed on the surfaces of the pellets to obtain rumen bypass glucose particles, or glucose and auxiliary materials are uniformly mixed by a method, water is used for preparing, granulating, shot-blasting and drying are carried out, screening is carried out to obtain particles, and then the molten rumen bypass fat powder is sprayed on the surfaces of the particles by adopting a fluidized granulating and coating machine bottom spraying process to obtain the rumen bypass glucose.

The main problems of the second type of method are: is not suitable for granulating part of nutrients. For example, glucose, during extrusion and granulation, the glucose, an extruder and a screw are rubbed to generate heat, and the glucose becomes sticky when meeting heat, so that a cutter is stuck during cutting, and particle cuts are uneven, so that the continuous production is not suitable. In addition, the daily ration passes through the rumen and is a continuous and dynamic process, and the method is easy to cause that nutrients passing through the rumen are coated, the nutrient rumen passes through the rumen at high rate but cannot be released in the small intestine, and then the nutrients passing through the rumen are degraded by rumen microorganisms due to overlong residence time in the rumen, so that the total bioavailability is low.

Disclosure of Invention

Based on the above, the application provides a rumen bypass preparation which can effectively pass through the rumen and can be fully released in the small intestine to be absorbed and utilized by animals, and a preparation method and application thereof.

In a first aspect of the present application, a method for preparing a rumen bypass preparation is provided, comprising the steps of:

emulsifying the nutrients with grease, an emulsifying agent and water to prepare an oil-in-water emulsifying system;

granulating by using the oil-in-water emulsion system to prepare a core;

emulsifying the nutrients with oil, an emulsifying agent and water to prepare a water-in-oil emulsifying system;

coating the surface of the core with the water-in-oil emulsifying system to prepare a first coating;

and melting the grease, and coating the melted grease on the surface of the first coating to prepare a second coating.

In some embodiments, the nutrient is one or more of glucose, choline, lysine, methionine, and niacin.

In some of these embodiments, the step of preparing the core has at least one of the following features:

(1) The mass ratio of the emulsifying agent, the grease and the nutrient is (1-10): 3-38): 100, and optionally (1-4): 7-18): 100;

(2) The mass ratio of the water to the nutrients is (25-100) 100;

(3) The emulsifier is one or more of polyoxyethylene (8) stearate, polyoxyethylene (20) glyceryl stearate, polyoxyethylene sorbitan esters and fatty acid salts;

(4) The oil and fat is a fatty acid having 14 or more carbon atoms; further optionally, the oil is one or more of stearic acid, soybean oil, rapeseed oil, sunflower oil, cottonseed oil, rice bran oil, corn oil, peanut oil, coconut oil, hydrogenated palm oil, rice bran wax, beeswax and carnauba wax.

In some of these embodiments, preparing the core comprises the steps of:

mixing the water and the nutrients at the temperature of 60-80 ℃ to prepare a water phase;

mixing the emulsifier with the melted grease to prepare an oil phase;

and adding the oil phase into the water phase, and emulsifying to prepare the oil-in-water emulsion system.

In some of these embodiments, the process of preparing the aqueous phase further comprises the step of adding a thickener;

optionally, the thickener comprises one or more of xanthan gum, acacia, pectin, acid modified starch, sodium carboxymethyl cellulose and hydroxypropyl methyl cellulose;

alternatively, the mass ratio of the thickener to the nutrient is (0.5-7): 100, alternatively (1-4): 100.

In some of these embodiments, the method of granulation is spray granulation;

optionally, the conditions of spray granulation include: the granulating temperature is 10-25 ℃, the feeding flow is 600-1200 kg/h, and the air inlet humidity is 0-5%.

In some of these embodiments, the granulation is completed and further comprising a step of drying;

optionally, the drying conditions include: the temperature is 40-50 ℃ and the time is 30-60 min.

In some of these embodiments, the step of preparing the first coating has at least one of the following characteristics:

(1) The mass ratio of the grease, the emulsifying agent and the nutrient is (100-233): 1.5-12): 100, and optionally (115-223): 3-10): 100;

(2) The mass ratio of the water to the nutrients is (25-100) 100;

(3) The emulsifier is one or more of monoglyceride, diglyceride monostearate, acetic acid fatty acid glyceride, lactic acid fatty acid glyceride, fatty acid propylene glycol ester, stearoyl lactylate and sorbitan esters;

(4) The grease is fatty acid with more than or equal to 14 carbon atoms; further optionally, the grease is one or more of stearic acid, glyceryl tristearate, coconut oil, hydrogenated palm oil, beeswax and carnauba wax.

In some of these embodiments, preparing the first coating comprises the steps of:

mixing the water with the nutrients at 60-80 ℃ to prepare a water phase;

mixing the emulsifier with the melted grease to prepare an oil phase;

adding the water phase into the oil phase for emulsification to prepare the water-in-oil emulsification system;

and coating the surface of the core with the water-in-oil emulsifying system at the temperature of 30-60 ℃, and cooling to prepare a first coating.

In some of these embodiments, in the step of preparing the second coating, the lipid is a fatty acid having greater than or equal to 14 carbon atoms; further optionally, the grease is one or more of stearic acid, glyceryl tristearate, coconut oil, hydrogenated palm oil, beeswax and carnauba wax.

In some of these embodiments, preparing the second coating comprises the steps of:

and (3) coating the melted grease on the surface of the first coating at the temperature of 40-80 ℃.

In a second aspect of the present application, there is provided a rumen bypass preparation prepared by the preparation method of the first aspect.

In some of these embodiments, the rumen bypass formulation has at least one of the following characteristics:

(1) The total weight of the nutrients accounts for 40-70 percent of the total weight of the rumen bypass preparation, and is optionally 45-67 percent;

(2) The particle size of the rumen bypass preparation is 0.8 mm-1.4 mm;

(3) The rumen bypass preparation has the moisture content of 0-3%.

In a third aspect of the present application, there is provided the use of the rumen bypass preparation of the second aspect in the preparation of a ration for an animal.

According to the preparation method of the rumen bypass preparation, the oil-in-water emulsion system is used for preparing the core, so that water-soluble nutrients are uniformly dispersed in the core, and the nutrient content of the whole preparation can be effectively improved; the first coating layer is coated on the surface of the core by a water-in-oil emulsion system, so that the rumen passing rate of the preparation can be increased together by reducing the specific surface area of the core and improving the oil content, and meanwhile, the coating amount of the outer layer oil is reduced, so that the small intestine release rate of the preparation is improved; and then the surface of the first coating is directly coated with grease to form a second coating, so that nutrients and rumen fluid are thoroughly separated, and the rumen passing rate of the preparation is effectively improved. The rumen bypass preparation prepared by the method has high nutrient content, little degradation of grease in rumen and easy degradation of lipase in small intestine, and when the preparation sequentially passes through rumen, abomasum and small intestine, the two layers of coatings have good protection effect on active substances in the rumen, so that the rumen passing rate is improved, the grease in the coatings is fully decomposed when reaching the small intestine, the small intestine release rate of the nutrients is improved, and the bioavailability is further improved.

In addition, the preparation method of the rumen bypass preparation has the following advantages:

(1) The prepared rumen bypass preparation has uniform particles, good fluidity and good physical form, is easy to directly use in production, effectively reduces the incidence rate of ketosis and fatty liver in the perinatal period of dairy cows, improves the health condition of animals, and improves the milk yield and dairy quality of the dairy cows;

(2) The two layers of coating can effectively reduce the mechanical abrasion of the rumen bypass preparation during storage, transportation and mixing with feed; the second coating also enhances the water barrier effect of the formulation and resistance to rumen microorganisms;

(3) The grease in the core can not only reduce the use of water in the granulating process, but also enable the preparation to have a certain slow-release effect;

(4) The preparation method has simple steps and easy operation, can realize continuous production, and is convenient for popularization and application.

Drawings

Fig. 1 is a process flow diagram of a method of preparing a rumen bypass formulation in an example of the present application.

Detailed Description

The rumen bypass preparation of the present application, and the preparation method and application thereof are described in further detail below with reference to specific examples. This application may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

Herein, "one or more" refers to any one, any two, or any two or more of the listed items.

In this application, "first aspect," "second aspect," etc. are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or quantity, nor are they to be construed as implying an importance or quantity of technical features indicated. Moreover, "first," "second," etc. are for non-exhaustive list description purposes only, and it should be understood that no closed limitation on the number is made.

In the present application, the technical features described in an open manner include a closed technical scheme composed of the listed features, and also include an open technical scheme including the listed features.

In the present application, reference is made to numerical intervals, where the numerical intervals are considered to be continuous unless specifically stated, and include the minimum and maximum values of the range, and each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values of the range is included. Further, when multiple range description features or characteristics are provided, the ranges may be combined. In other words, unless otherwise indicated, all ranges disclosed herein are to be understood to include any and all subranges subsumed therein.

The percentage content referred to in the present application refers to mass percent for both solid-liquid and solid-solid phase mixing and volume percent for liquid-liquid phase mixing unless otherwise specified.

The percentage concentrations referred to in this application, unless otherwise indicated, refer to the final concentrations. The final concentration refers to the ratio of the additive component in the system after the component is added.

The temperature parameter in the present application is not particularly limited, and may be a constant temperature treatment or a treatment within a predetermined temperature range. The constant temperature process allows the temperature to fluctuate within the accuracy of the instrument control.

Room temperature in this application generally means 4 c to 30 c, preferably 20 c + 5 c.

In a first aspect of the present application, a preparation method of a rumen bypass preparation is provided, wherein a process flow chart of the preparation method is shown in fig. 1, and the preparation method comprises the following steps:

s1: firstly emulsifying nutrients, grease, an emulsifying agent and water to prepare an oil-in-water (O/W) emulsifying system;

s2: granulating by using the oil-in-water emulsion system to prepare a core;

s3: performing secondary emulsification on the nutrients, grease, an emulsifying agent and water to prepare a water-in-oil (W/O) emulsifying system;

s4: coating the surface of the core with the water-in-oil emulsifying system to prepare a first coating;

s5: and melting the grease, and coating the melted grease on the surface of the first coating to prepare a second coating.

It will be appreciated that the above numbering of S1-S5 is used only to more clearly describe embodiments of the present application and should not be taken as limiting the order of steps of the present application.

In some examples, the nutrient is a water-soluble nutrient. Without limitation, the nutrient is one or more of glucose, choline, lysine, methionine and niacin.

Specifically, steps S1 to S2 are steps for preparing a core.

In some examples, the mass ratio of the emulsifier, the fat, and the nutrient is (1-10): (3-38): 100. Specifically, the mass ratio includes, but is not limited to: 1:38:100, 10:38:100, 5:25:100, 1:3:100, 2:10:100, 3:10:100, 4:20:100, 2.3:9.6:100, 2.1:10.5:100, 3.2:13.2:100, 3.25:14.5:100, or ranges therebetween. Further, the mass ratio of the emulsifying agent, the grease and the nutrient is (1-4): (7-18): 100.

It is understood that the water is added when the water-phase substances such as the nutrients are dissolved in the preparation of the oil-in-water emulsion system and/or is contained in the raw materials in the form of an aqueous solution. The mass ratio of the water to the nutrients may be, without limitation, (25 to 100): 100.

It is understood that in the core, the emulsifier is an oil-in-water emulsifier. In some examples, the emulsifier is one or more of polyoxyethylene (8) stearate, polyoxyethylene (20) glyceryl stearate, polyoxyethylene sorbitan esters, and fatty acid salts in the core. Without limitation, polyoxyethylene sorbitan esters may be exemplified by one or more of tween 20, tween 40, tween 60, tween 65 and tween 80, and salts of fatty acid salts may be exemplified by one or more of potassium salt, sodium salt and calcium salt.

In some examples, the grease is a fatty acid having greater than or equal to 14 carbon atoms; further optionally, the oil is one or more of stearic acid, soybean oil, rapeseed oil, sunflower oil, cottonseed oil, rice bran oil, corn oil, peanut oil, coconut oil, hydrogenated palm oil, rice bran wax, beeswax and carnauba wax.

In some examples, preparing the core includes the steps of:

mixing the emulsifier with the melted grease to prepare an oil phase;

It will be appreciated that the material is dissolved and clarified during mixing.

Without limitation, the emulsion is homogenized and stabilized by using a shear emulsification method.

Further, the process of preparing the aqueous phase further comprises the step of adding a thickener. The thickening agent can increase the stability and viscosity of the emulsion, and the increase of the viscosity of the emulsion can increase the particle size of particles during granulation.

In some examples, the thickening agent includes one or more of xanthan gum, acacia gum, pectin, acid-modified starch, sodium carboxymethyl cellulose, and hydroxypropyl methyl cellulose.

In some examples, the mass ratio of the thickener to the nutrient is (0.5-7): 100. Specifically, the mass ratio includes, but is not limited to: 0.5:100, 1:100, 1.2:100, 1.3:100, 2:100, 2.75:100, 3:100, 4:100, 5:100, 6:100, 7:100, or a range therebetween. Further, the mass ratio of the thickener to the nutrient is (1-4): 100.

In some examples, the method of granulation is spray granulation.

Further, the conditions of spray granulation include: the granulating temperature is 10-25 ℃, the feeding flow is 600-1200 kg/h, and the air inlet humidity is 0-5%.

Specifically, the granulation temperature includes, but is not limited to: 10 ℃, 13 ℃, 15 ℃, 17 ℃, 18 ℃, 20 ℃, 23 ℃, 25 ℃, or a range between any two of the foregoing. Further, the granulating temperature is 15-20 ℃.

Specifically, feed flows include, but are not limited to: 600kg/h, 700kg/h, 800kg/h, 900kg/h, 1000kg/h, 1100kg/h, 1200kg/h or a range between any two of the foregoing. Further, the feed flow rate is 800kg/h to 1000kg/h.

Specifically, the intake air humidity includes, but is not limited to: 0%, 1%, 2%, 3%, 4%, 5% or a range between any two of the foregoing. Further, the inlet air humidity is 0% -3%.

In some examples, the granulation is completed with a drying step. Further, the drying conditions include: the temperature is 40-50 ℃ and the time is 30-60 min. Drying may employ, without limitation, fluidized bed drying.

Specifically, temperatures include, but are not limited to: 40 ℃, 41 ℃, 42 ℃, 43 ℃, 44 ℃, 45 ℃, 46 ℃, 47 ℃, 48 ℃, 49 ℃, 50 ℃, or a range between any two of the foregoing.

Specifically, the time includes, but is not limited to: 30min, 35min, 40min, 45min, 50min, 55min, 60min, or a range therebetween. Further, the time is 40 min-50 min.

Specifically, steps S3 to S4 are steps for preparing the first coating layer.

In some examples, the mass ratio of the fat, the emulsifier, and the nutrient is (100-233): (1.5-12): 100. Specifically, the mass ratio includes, but is not limited to: 100:1.5:100, 133:8:100, 233:12:100, 150:8:100, 170:6:100, 200:8:100, 122:5.3:100, 126:5.6:100, 166.7:7.5:100, 210:9:100, or ranges therebetween. Further, the mass ratio of the grease to the emulsifying agent to the nutrient is (115-223): 3-10): 100.

It is understood that the water is added when the water-phase substances such as the nutrients are dissolved in the preparation of the water-in-oil emulsion system and/or is contained in the raw materials in the form of an aqueous solution. The mass ratio of the water to the nutrients may be, without limitation, (25 to 100): 100.

It will be appreciated that in the first coating, the emulsifier is a water-in-oil emulsifier. In some examples, the first coating layer comprises one or more of a monoglyceride, a glyceryl monostearate, a glyceryl acetate, a glyceryl lactate, a propylene glycol fatty acid ester, a stearyl lactate, and a sorbitan ester. Among them, sorbitan esters can be exemplified by one or more of span 20, span 40, span 60, span 65, and span 80.

In some examples, the first coating is wherein the lipid is a fatty acid having greater than or equal to 14 carbon atoms; further optionally, the grease is one or more of stearic acid, glyceryl tristearate, coconut oil, hydrogenated palm oil, beeswax and carnauba wax.

In some examples, preparing the first coating includes the steps of:

mixing the water with the nutrients at 60-80 ℃ to prepare a water phase;

mixing the emulsifier with the melted grease to prepare an oil phase;

Specifically, step S5 is a step of preparing a second coating.

In some examples, the oil is a fatty acid having greater than or equal to 14 carbon atoms in the step of preparing the second coating. Further optionally, the grease is one or more of stearic acid, glyceryl tristearate, coconut oil, hydrogenated palm oil, beeswax and carnauba wax. Without limitation, the grease is heated to 80 ℃ to 100 ℃ and melted during the process of preparing the second coating. It will be appreciated that the content of trans fatty acids in the fat should be less than 1%.

In some examples, preparing the second coating includes the steps of: and (3) coating the melted grease on the surface of the first coating at the temperature of 40-80 ℃.

Other examples of the present application provide rumen bypass preparations prepared by the preparation method described above.

In some examples, the total weight of the nutrients is 40% -70% of the total weight of the rumen bypass formulation. Specifically, the total weight percentages of nutrients include, but are not limited to: 40%, 43%, 45%, 47%, 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70% or a range therebetween. Further, the total weight percentage of the nutrients is 45-67% based on the total weight of the rumen bypass preparation.

In some examples, the rumen bypass formulation has a particle size of 0.8mm to 1.4mm. Specifically, particle sizes include, but are not limited to: 0.8mm, 0.9mm, 1.0mm, 1.1mm, 1.2mm, 1.3mm, 1.4mm, or a range therebetween. Further, the particle diameter is 0.9mm to 1.3mm.

In some examples, the rumen bypass preparation has a moisture content (mass percent) of 0 to 3%. Specifically, the moisture content includes, but is not limited to: 0. 0.5%, 1%, 1.5%, 2%, 2.5%, 3% or a range between any two of the foregoing. Further, the moisture content is 0 to 2.5%.

Further examples of the present application provide for the use of a rumen bypass formulation as described above in the preparation of a ration for an animal. Further, the animal is a ruminant animal having a rumen. Still further, the animal is a cow. Without limitation, the rumen bypass preparation is added to animal ration as a feed additive.

The starting materials and reagents referred to in the following specific examples may be obtained commercially or may be prepared by known means by those skilled in the art.

Example 1:

a) Uniformly mixing 520kg of glucose and 7kg of acid modified starch, adding 150kg of purified water at 75-80 ℃ and stirring in an emulsifying stirring kettle to prepare a water phase; weighing 50kg of stearic acid, melting in an oil melting pot, adding 12kg of Tween 80 until Tween 80 is completely dissolved, and preparing an oil phase; adding the oil phase into the water phase, shearing for 30min at 5m/s to obtain emulsion, granulating in a spray granulator at 18 ℃ with a feed flow of 800kg/h and an inlet air humidity of 3%; and drying the solid spherical glucose core particles for 45 minutes by a fluidized bed at the temperature of 45 ℃ to obtain the solid spherical glucose core particles, wherein the moisture content is 2.3%, and the particle size of the solid spherical glucose core particles is 0.6-1.0 mm.

b) Dissolving 150kg of glucose in 41kg of hot water at 75-80 ℃ to prepare a glucose solution; adding 8kg of emulsifier glyceryl monostearate into 183kg of molten glyceryl tristearate, stirring and dissolving to obtain clear and transparent oil phase, adding glucose solution into the oil phase, and shearing and emulsifying for 30min to obtain primary coating emulsion; and d, adding the glucose core particles prepared in the step a into a hot-melt coating machine, spraying the primary coating emulsion at 45 ℃, coating the primary coating, discharging and cooling to room temperature to obtain particles with the particle size of 0.8-1.2 mm and the moisture content of 2.0%.

c) Melting 70kg of hydrogenated palm oil at 90 ℃, spraying the melted hydrogenated palm oil as an outer coating liquid at 55 ℃ on the surface of the particles obtained in the step b) for carrying out a second coating to obtain rumen bypass glucose particles, wherein the particle size of the particles is 0.9-1.3 mm, the glucose content is 66.0%, and the moisture content is 1.5%.

Wherein, the particle size is measured by a Markov particle size meter, the glucose content is measured according to GB 5009.8-2016 determination of fructose, glucose, sucrose, maltose and lactose in food, and the moisture content is measured by 105 ℃ oven method.

Example 2:

a) Uniformly mixing 420kg of glucose and 5kg of Arabic gum, adding 110kg of purified water at 75-80 ℃ and stirring in an emulsifying stirring kettle to prepare a water phase; weighing 44kg of soybean oil, adding 9kg of polyoxyethylene (20) glycerol stearate, and stirring until the glycerol stearate is completely dissolved to prepare an oil phase; adding the oil phase into the water phase, shearing for 30min at 5m/s to obtain emulsion, granulating in a spray granulator at 15 ℃ with a feed flow rate of 900kg/h and an inlet air humidity of 1%; and drying the solid spherical glucose core particles for 40 minutes by a fluidized bed at the temperature of 40 ℃ to obtain the solid spherical glucose core particles, wherein the moisture content is 1.2%, and the particle size of the solid spherical glucose core particles is 0.6-1.0 mm.

b) 180kg of glucose is dissolved in 50kg of hot water at 75-80 ℃ to prepare a glucose solution; adding 10kg of lactic acid fatty acid glyceride into 227kg of molten carnauba wax, stirring and dissolving to obtain a clear and transparent oil phase, adding a glucose solution into the oil phase, and shearing and emulsifying for 30min to obtain a primary coating emulsion; and c, adding the glucose core particles prepared in the step a into a hot-melt coating machine, spraying the primary coating emulsion at 60 ℃, coating an inner layer, discharging and cooling to room temperature to obtain particles with the particle size of about 0.8-1.2 mm and the water content of 1.0%.

c) And (2) melting 105kg of carnauba wax at 90 ℃, spraying the molten carnauba wax serving as an outer coating liquid on the surface of the particle obtained in the step b) at 78 ℃ to carry out outer coating, so as to obtain rumen bypass glucose particles, wherein the particle size of the particles is about 0.9-1.3 mm, the glucose content is 59.6%, and the moisture content is 0.7%.

Example 3:

a) Adding 6kg of sodium carboxymethyl cellulose and 507kg of 75% (mass percent) choline aqueous agent into an emulsifying stirring kettle, heating and stirring at 75-80 ℃ until the sodium carboxymethyl cellulose and the choline aqueous agent are completely dissolved, and preparing a water phase; weighing 50kg of carnauba wax, dissolving in an oil dissolving pot, adding 12kg of sodium fatty acid until the sodium fatty acid is completely dissolved, and preparing an oil phase; adding the oil phase into the water phase, shearing for 30min at 5m/s to obtain emulsion, and granulating in a spray granulator at 20 ℃ with a feed flow of 1000kg/h and an inlet air humidity of 2%; and drying the mixture for 45 minutes by a fluidized bed at 50 ℃ to obtain solid spherical choline core particles, wherein the moisture content is 2.9%, and the particle size of the particles is about 0.6-1.0 mm.

b) Adding 9kg of glyceryl monostearate into 200kg of molten beeswax, stirring and dissolving to obtain a clear and transparent oil phase, adding 160kg of 75% (mass percent) choline aqueous agent preheated to 75-80 ℃, and shearing and emulsifying for 30min to obtain a primary coating emulsion; and c, adding the choline core particles prepared in the step a into a hot-melt coating machine, spraying the primary coating emulsion at 55 ℃, coating an inner layer, discharging and cooling to room temperature to obtain particles with the particle size of about 0.8-1.2 mm and the water content of 2.5%.

c) Melting 132kg beeswax at 90 deg.C, spraying onto the surface of the granule obtained in step b) as outer coating liquid at 60 deg.C, and coating to obtain rumen-bypass choline granule with particle diameter of about 0.9-1.3 mm, wherein choline content is 53.8% and water content is 2.2%.

Example 4:

a) Adding 11kg of acid modified starch into 800kg of 50% (mass percent) L-lysine water solution, and stirring at 75-80 ℃ until the acid modified starch is completely dissolved to prepare a water phase; weighing 58kg of corn oil, adding 13kg of sodium fatty acid, and stirring until the sodium fatty acid is completely dissolved to prepare an oil phase; adding the oil phase into the water phase, shearing for 30min at 5m/s to obtain emulsion, granulating in a spray granulator at 15 ℃ with a feed flow of 800kg/h and an inlet air humidity of 1%; and drying the mixture for 50 minutes by a fluidized bed at 50 ℃ to obtain solid spherical core particles, wherein the moisture content is 1.5%, and the particle size of the particles is about 0.6-1.0 mm.

b) Adding 9kg of mono-diglyceride into 210kg of melted hydrogenated palm oil, stirring and dissolving to obtain a clear and transparent oil phase, adding 200kg of 50% (mass percent) L-lysine water solution preheated to 75-80 ℃, and shearing and emulsifying for 30min to obtain a primary coating emulsion; and c, adding the lysine core particles prepared in the step a into a hot-melt coating machine, spraying the primary coating emulsion at 50 ℃, coating an inner layer, discharging and cooling to room temperature to obtain particles with the particle size of 0.8-1.2 mm and the water content of 1.3%.

c) And (b) melting 125kg of hydrogenated palm oil at 90 ℃, spraying the melted hydrogenated palm oil as an outer coating liquid at 55 ℃ on the surface of the particle obtained in the step b) to perform outer coating to obtain rumen-bypass lysine particles with the particle size of about 0.9-1.3 mm, wherein the lysine content is 53.5% and the water content is 1.0%.

Comparative example 1:

the comparative example provides rumen bypass glucose particles which are prepared by adopting a spray granulation method and comprise the following steps:

a) Mixing 510kg of anhydrous glucose and 10kg of light calcium carbonate uniformly, pouring the mixture into 450kg of completely melted solid vegetable oil, homogenizing by a homogenizer, and controlling the temperature of the materials at 90-100 ℃ for standby.

b) The material is made to pass through a centrifugal atomizer through a condensation spray granulation technology to form regular spherical liquid drops, and the particles are solidified after being contacted with low-temperature air of 0-20 ℃ in a tower to form regular spherical particles.

c) And (3) screening particles with 60-14 meshes, and mixing with 1.5% of white carbon black to obtain rumen bypass glucose particles with the glucose content of about 50%.

Comparative example 2:

the comparative example provides rumen bypass glucose granules which are prepared by adopting a coating method after extrusion granulation, and the steps are as follows:

a) Mixing 600kg of glucose, 50kg of silicon dioxide and 2.65kg of sodium carboxymethyl cellulose, adding 80kg of water, preparing the mixture into a pelletizable state, granulating by a granulator, then performing shot blasting by a shot blasting machine, and drying to obtain glucose granules;

b) After 340kg of hydrogenated palm oil is dissolved at 90 ℃, the mixture is sprayed as a coating liquid on the surface of the particles obtained in the step a) to carry out coating, and rumen bypass glucose particles with the glucose content of about 60% are obtained.

Test example:

(1) Rumen bypass rate determination of rumen bypass formulations

Rumen bypass rate of rumen bypass preparation samples of fistula cattle detection examples and comparative examples were used, and the experimental contents are as follows: nylon filter cloth with 50 μm holes was selected to prepare a nylon bag of 12cm×8cm (length×width). 10.0g rumen bypass preparation samples are accurately weighed in a nylon bag, the mouth of the nylon bag is tightly tied by rubber bands, and 3 repetitions are arranged for each time point of each cow. The samples were fed before feeding in the morning and cultured for 4h, 8h, 12h, 16h, 24h, 36h and 48h, and the rumen bypass rate of the samples in the cows was calculated. According to the national standard, the residual iodine method is adopted to measure the corresponding nutrient content in the feed additive.

The test results are shown in table 1 below.

TABLE 1 rumen bypass rate of rumen bypass formulation (%)

As can be seen from Table 1, the rumen bypass rates of the samples of examples 1 to 4 were significantly increased as compared with comparative examples 1 and 2.

(2) Small intestine digestibility assay of rumen bypass preparation

The small intestine digestibility of the rumen bypass preparation samples of the examples and the comparative examples was determined by a semi-in vitro three-step method, and the specific method is as follows:

after the rumen preparation sample is fermented and degraded in rumen for 16 hours, a certain amount of rumen non-degradation residue sample is collected, 1g of rumen non-degradation residue sample is weighed and put into a nylon bag (5 cm multiplied by 10 cm), and a 2L conical flask is hatched for at most 12 bags each time. 2L of hydrochloric acid solution with pH=1.9 containing 1g/L pepsin (P-7012, sigma Co., USA, activity not less than 2 500U/mg prot, potency 100%) was placed in the conical flask, and the solution was preheated in advance. And incubated constantly for 1h at 39 ℃. After the cultivation, the nylon bag is taken out, drained and washed with tap water until the nylon bag is clear, placed in the fistula of the small intestine for cultivation for 24 hours, washed cleanly, dried at 40 ℃ until the weight is constant, and the release rate of the sample in the small intestine is measured according to the feed nutrient disappearance rate in the nylon bag.

Small intestine release rate = (corresponding nutrient content in rumen bypass preparation-corresponding nutrient content in residue after enzymolysis)/corresponding nutrient content in rumen bypass preparation×100%

TABLE 2 Small intestine Release Rate of rumen bypass preparation (%)

	Small intestine release rate
		Example 1	97.85±1.38
Example 2	94.22±1.53
		Example 3	90.09±1.01
Example 4	91.32±1.30
		Comparative example 1	96.78±1.01
Comparative example 2	89.57±1.16

As can be seen from table 2, the sample of example 1 can have a small intestine release rate comparable to that of comparative examples 1 and 2, and even superior small intestine release rates, and examples 2, 3 and 4 are still higher and can satisfy the desired bioavailability and therapeutic effect, although the small intestine release rate is reduced as compared with comparative example 1.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples merely represent a few embodiments of the present application, which facilitate a specific and detailed understanding of the technical solutions of the present application, but are not to be construed as limiting the scope of the claims. It should be noted that it would be apparent to those skilled in the art that various modifications and improvements could be made without departing from the spirit of the present application, which would be within the scope of the present application. It should be understood that those skilled in the art, based on the technical solutions provided in the present application, can obtain technical solutions through logical analysis, reasoning or limited experiments, all fall within the protection scope of the claims attached in the present application. The scope of the patent application is therefore intended to be indicated by the appended claims, and the description may be used to interpret the contents of the claims.

Claims

1. The preparation method of the rumen bypass preparation is characterized by comprising the following steps:

granulating by using the oil-in-water emulsion system to prepare a core;

2. The method for preparing the rumen bypass preparation according to claim 1, wherein the nutrient is one or more of glucose, choline, lysine, methionine and nicotinic acid.

3. The method of preparing a rumen bypass formulation according to claim 1, wherein the step of preparing the core has at least one of the following characteristics:

(2) The mass ratio of the water to the nutrients is (25-100) 100;

4. The method for preparing the rumen bypass preparation according to claim 1, wherein the preparation of the core comprises the steps of:

mixing the emulsifier with the melted grease to prepare an oil phase;

5. The method for preparing rumen bypass preparation according to claim 4, wherein the process for preparing the aqueous phase further comprises a step of adding a thickener;

6. The method for preparing the rumen bypass preparation according to claim 1, wherein the granulating method is spray granulating;

7. The method for preparing the rumen bypass preparation according to claim 1, wherein the preparation method further comprises a step of drying after granulation is finished;

8. The method of preparing a rumen bypass formulation according to any one of claims 1 to 7, wherein the step of preparing the first coating has at least one of the following characteristics:

(2) The mass ratio of the water to the nutrients is (25-100) 100;

9. The method for preparing a rumen bypass preparation according to any one of claims 1 to 7, wherein preparing the first coating comprises the steps of:

mixing the water with the nutrients at 60-80 ℃ to prepare a water phase;

mixing the emulsifier with the melted grease to prepare an oil phase;

10. The method for preparing a rumen bypass preparation according to any one of claims 1 to 7, wherein in the step of preparing the second coating, the oil is a fatty acid having 14 or more carbon atoms; further optionally, the grease is one or more of stearic acid, glyceryl tristearate, coconut oil, hydrogenated palm oil, beeswax and carnauba wax.

11. The method for preparing a rumen bypass preparation according to any one of claims 1 to 7, wherein preparing the second coating comprises the steps of:

12. The rumen bypass preparation prepared by the preparation method of any one of claims 1 to 11.

13. The rumen bypass formulation of claim 12, having at least one of the following characteristics:

(2) The particle size of the rumen bypass preparation is 0.8 mm-1.4 mm;

(3) The rumen bypass preparation has the moisture content of 0-3%.

14. Use of the rumen bypass preparation of claim 12 or 13 for preparing a ration for an animal.