CN117598926A - A skin barrier repair composition containing recombinant collagen and its application - Google Patents

Abstract

The invention relates to a skin barrier repair composition containing recombinant collagen and its application. The skin barrier repair composition includes recombinant collagen, conotide, schizophyllan and ectoin. The invention creatively combines four components: recombinant collagen, conotide, schizophyllan and ectoin. The four components comprehensively improve the problem of skin barrier damage from different dimensions while strengthening the skin barrier. The four components complement each other and have potential mutual promotion effects. They have a synergistic effect on the above-mentioned effects and can effectively improve comprehensive skin problems caused by damaged skin barriers.

Description

A skin barrier repair composition containing recombinant collagen and its application

Technical field

The invention belongs to the technical field of cosmetics and relates to a skin barrier repair composition containing recombinant collagen and its application.

Background technique

Healthy skin can effectively retain moisture, thereby maintaining good skin elasticity and shiny appearance. However, the skin is affected by the living environment and its own physiological conditions. The skin may suffer from sensitivity, dryness and peeling, excessive oil secretion, spots, sagging and aging skin, redness and other skin problems. The main reason is that the skin barrier is damaged. The skin barrier is a protective structure composed of the stratum corneum and the sebum film. It can buffer physical and chemical stimulation, effectively inhibit bacterial growth, block the damage to the skin caused by bacteria, viruses, and fungi, and can lock in moisture and prevent moisture from evaporating. , thereby achieving the function of retaining moisture. The skin barrier is damaged, causing the skin to be eroded by bacteria, viruses, and fungi. It will also cause the water in the skin to evaporate unbridled, causing various skin problems, such as sensitivity, dry skin, sagging and aging skin, etc.

The commonly used method to repair the skin barrier is to fully hydrate. However, most of the water in traditional cosmetics only exists on the surface of the skin. The evaporation rate of water on the surface of the skin is much faster than the absorption rate. The amount of water that can be absorbed by the skin is limited, resulting in skin barrier The repair speed is slow and takes a long time, and simple hydration has no effective improvement effect on skin sensitivity problems and skin sagging and aging.

CN116172934A discloses a composition with both skin barrier repair function and hydration function and its application, including the following components by mass: 0.1-20 parts of Cyperus rotundus root extract, 0.3-20 parts of Boswellia serrata extract, no. 0.2-20 parts of medicinal leaf cell extract, 1-30 parts of glycerin, 10-90 parts of deionized water. The composition has obvious effects on relieving dry skin, restoring skin epidermal barrier, and increasing skin moisture content. However, this composition repairs the skin barrier slowly and takes a long time, and it cannot effectively improve the comprehensive skin problems caused by the damaged skin barrier.

CN109394595A discloses a facial mask essence with deep hydration and barrier repair. The weight ratio of the formula is bifid yeast fermentation product filtrate: 10-97%, purified water: 0-90%, and allantoin: 0.1-0.2%. Polyol: 1-8%, polyglycerol humectant: 0.5-2%, disodium EDTA: 0.02-0.1%, cinnamonone: 0.3-0.6%, 1,2-hexanediol: 0.3-0.6%, card Bohm: 0.1-0.2%, Arginine: 0.1-0.2%, Sodium Hyaluronate: 0.01-0.1%, Hydroxyethylcellulose: 0.01-0.1%, Hydroxylated Lecithin: 0.1-0.2%; with deep It has the effect of hydrating and repairing the barrier. However, this product repairs the skin barrier slowly and takes a long time, and it cannot effectively improve the comprehensive skin problems caused by damaged skin barrier.

Therefore, it is very necessary to develop a product that can effectively improve the comprehensive skin problems caused by damaged skin barrier.

Contents of the invention

In view of the shortcomings of the existing technology, the object of the present invention is to provide a skin barrier repair composition containing recombinant collagen and its application.

In order to achieve the purpose of this invention, the present invention adopts the following technical solutions:

In a first aspect, the present invention provides a skin barrier repair composition containing recombinant collagen. The skin barrier repair composition includes recombinant collagen, conotide, schizophyllan and ectoin.

Conotide can accelerate local blood circulation and local metabolism, stimulate skin activity, enhance the activity of skin cells, and delay skin aging. Its ingredients are rich in nutrients and have excellent skin care effects. Schizophyllum can activate the activity of skin immune cells and other tissue cells, improve the resistance and repair ability of the entire skin tissue, and also has good skin moisturizing function. Ectoin has great potential in anti-aging, anti-ultraviolet, anti-oxidation, moisturizing and inhibiting inflammation. It can improve the immune protection ability of skin cells and has good anti-allergic and anti-inflammatory effects; it increases cell repair ability, It enables the skin to effectively resist the invasion of microorganisms and allergens, has good protection and repair effects on the skin, and accelerates the recovery of skin health. In terms of fighting against ultraviolet rays, Ectoin can repair cell DNA damage caused by ultraviolet rays, effectively reduce the generation of sunburned cells, and prevent damage to cell membrane structures. Collagen can replenish collagen to the skin in a timely manner, strengthen the skin barrier, resist environmental damage, and keep the skin in a healthy state.

The invention creatively combines four components: recombinant collagen, conotide, schizophyllan and ectoin. The four components comprehensively improve the problem of skin barrier damage from different dimensions while strengthening the skin barrier. The four components complement each other and have potential mutual promotion effects. They have a synergistic effect on the above-mentioned effects and can effectively improve comprehensive skin problems caused by damaged skin barriers.

Preferably, the skin barrier repair composition includes 0.01-2 parts of recombinant collagen, 0.1-3 parts of conotide, 0.1-0.5 parts of Schizophyllan and 0.1-1 part of ectoin in terms of parts by mass.

Based on the potential interactions between the above ingredients, when the above four ingredients are combined in a specific mass ratio, they have better effects in strengthening the skin barrier and improving comprehensive skin problems caused by damaged skin barrier. .

The mass parts of the recombinant collagen can be selected from 0.01 part, 0.03 part, 0.05 part, 0.08 part, 0.1 part, 0.2 part, 0.4 part, 0.5 part, 0.8 part, 1 part, 1.2 part, 1.4 part, 1.5 part , 1.8 parts, 2 parts, etc.; the mass parts of the conopeptide can be selected from 0.1 parts, 0.3 parts, 0.5 parts, 0.8 parts, 1 part, 1.2 parts, 1.4 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.5 parts, 2.8 parts, 3 parts, etc.; the mass parts of the Schizophyllan polysaccharide can be selected from 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, 0.45 parts, 0.5 parts, etc.; the mass parts of ectoine can be selected from 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, etc. . Other specific point values within the above-mentioned numerical ranges can be selected, so I will not go into details here.

Preferably, the nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID NO: 1.

Compared with collagen or recombinant collagen from other sources, the present invention also found that the recombinant collagen from the above specific source is more effective in strengthening the skin barrier and improving comprehensive skin problems caused by damaged skin barrier.

SEQ ID NO:1：

GGGCCTCAAGGTATTGCTGGACAGCGTGGTGTGGTCGGCCTGCCTGGTCAGAGAGGAGAGAGGCTTCCCTGGTCTTCCTGGCCCCTCTGGTGAACCTGGCAAACAAGGTCCCTCTGGAGCAAGTGGTGAACGTGGTCCCTCTGGTCCCATGGGCCCCTGGATTGGCTGGACCCCCTGGTGAATCTGGACGTGAGGGGGCTCCTGGTGCCGAAGGTTCCCCTGGACGAGACGGTTCTCCTGGCGCCAAGGGTG ACCGTGGTGAGACCGGCCCCGCTGGACCCCCTGGTGCTCCTGGTGCTCCTGGTGCCCCTGGCCCCGTTGGCCCTGCTGGCAAGAGTGGTTGATCGTGGTGAGACTGGTCCTGCTGGTCCCGCCGGAGAACGAGGTGGCCCTGGAGGACCTGGCCCTCAGGGTCCTCCTGGAAAGAATGGTGAAACTGGACCTCAGGGACCCCCAGGGCCTACTGGGCCTGGTGGTGACAAAGGAGACACAGGACCCTGGTCCACAAGGATTACAAGGC TTGCCTGGTACAGGTGGTCCTCCAGGAGAAAATGGAAAACCTGGGGAACCAGGTCCAAAGGGTGATGCCGGTGCACCTGGAGCTCCAGGAGGCAAGGGTGATGCTGGTGCCCCTGGTGAACGTGGACCTCCTGGATTGGCAGGGGCCCCAGGACTTAGAGGTGGAGCTGGTCCCCCTGGTCCCGAAGGAGGAAAGGGTGCTGCTGGTCCTCCTGGGCCACCTGGTGCTGCTGGTACTCCTGGTCTGCAAGGAATGCCTGGACC TGGTCCTTGCTGTGGTGGTTAA.

In a second aspect, the present invention provides the use of the skin barrier repair composition containing recombinant collagen according to the first aspect in the preparation of cosmetics.

Preferably, the mass percentage of the skin barrier repair composition containing recombinant collagen in the cosmetic is 0.1-15%, such as 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 10%, 11%, 12%, 13%, 15%, etc. Other specific point values within this numerical range can be selected and will not be described one by one here.

In a third aspect, the present invention provides an emulsion that strengthens skin barrier repair. The raw materials for preparing the emulsion include, in terms of mass percentage: 0.1-15% of the skin barrier repair composition containing recombinant collagen described in the first aspect. , oil phase 1-20%, emulsifier 1-10% and water 50-90%.

The mass percentage of the skin barrier repair composition can be selected from 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 10%, 11%, 12% , 13%, 15%, etc.; the mass percentage of the oil phase can be selected from 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 15%, 18%, 20%, etc.; the mass percentage of the emulsifier can be selected from 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8 %, 9%, 10%, etc.; the mass percentage of water can be selected from 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, etc. Other specific point values within the above-mentioned numerical ranges can be selected, so I will not go into details here.

Preferably, the oil phase includes glyceryl caprylate, squalane, oleocanthal oil, albacore oil, jojoba oil, avocado butter, sweet almond oil, grape seed oil, caprylic/capric triglyceride , any one or a combination of at least two of hydrogenated coconut glyceryl, phytosterol isostearate, isononyl isononate, arachidyl alcohol, vitamin A, vitamin E or vitamin F. A combination of white pond flower seed oil, sweet almond oil, grape seed oil and vitamin E is preferred.

Preferably, the emulsifier includes glycerol stearate, cetearyl alcohol, sodium stearoyl lactylate, glyceryl stearate citrate, cholesterol, cetearyl olive oil, sorbate Any one or a combination of at least two of polyglyceryl oleate, sorbitan stearate, polyglycerol-10 oleate or polysorbate-60.

Preferably, the raw materials for preparing the emulsion also include, in terms of mass percentage: 1-15% plant-derived conditioner, 0.1-2% pH regulator, 0.01-0.5% chelating agent, 0.05-0.5% antioxidant, aromatic Any one or a combination of at least two agents 0.1-0.5%.

The mass percentage of the plant-derived conditioner can be selected from 1%, 2%, 3%, 4%, 5%, 6%, 7%, 10%, 11%, 12%, 13%, 15%, etc. ; The mass percentage of the pH regulator can be selected from 0.1%, 0.3%, 0.5%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, etc.; The chelating agent The mass percentage content can be selected as 0.01%, 0.03%, 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.3%, 0.4%, 0.5%, etc.; the mass percentage content of the antioxidant can be selected as 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.3%, 0.4%, 0.5%, etc.; the mass percentage of the fragrance can be selected from 0.1%, 0.15%, 0.2%, 0.3%, 0.4 %, 0.5%, etc.; other specific point values within the above numerical range can be selected, so I will not go into details here.

Preferably, the plant-derived conditioning agent includes witch hazel extract and/or breviscapus extract.

The present invention also creatively found that when witch hazel extract and/or breviscapus extract are further added to the above products, especially the combination of witch hazel extract and breviscapus extract, the barrier repair effect of the product can be further improved. . Witch hazel extract and Erigeron flower extract also have a synergistic effect in exerting the above effects.

In a fourth aspect, the present invention provides a method for preparing an emulsion that strengthens skin barrier repair according to the third aspect, and the preparation method includes the following steps:

(1) Mix and homogenize water and the skin barrier repair composition at 25-40°C to obtain a water phase; mix and homogenize the oil phase and emulsifier at 35-50°C to obtain an oil phase;

(2) Mix and homogenize the water phase, the oil phase and the remaining preparation raw materials at 25-40°C to obtain the emulsion that strengthens skin barrier repair.

The preparation process of the emulsion for strengthening skin barrier repair involved in the present invention is simple and easy to operate, and is very suitable for industrial production.

The specific point values in 25-40°C can be selected as 25°C, 30°C, 35°C, 40°C, etc.; the specific point values in 35-50°C can be selected as 35°C, 40°C, 45°C, 50℃, etc.; other specific point values within the above numerical range can be selected, so I will not go into details here.

Compared with the prior art, the present invention has the following beneficial effects:

The invention creatively combines four components: recombinant collagen, conotide, schizophyllan and ectoin. The four components comprehensively improve the problem of skin barrier damage from different dimensions while strengthening the skin barrier. The four components complement each other and have potential mutual promotion effects. They have a synergistic effect on the above-mentioned effects and can effectively improve comprehensive skin problems caused by damaged skin barriers.

Detailed ways

In order to further explain the technical means adopted by the present invention and its effects, the technical solutions of the present invention will be further described below with reference to preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

The mass parts of each component in the composition product mentioned in the following content are calculated based on the actual active ingredient content in commercially available raw materials.

The witch hazel extract involved in the following content comes from the products of Lanzhou Waterless Biotechnology Co., Ltd.; the Erigeron extract comes from the products of Lanzhou Waterless Biotechnology Co., Ltd.; the Schizophyllum polysaccharide comes from Lanzhou Products of Waterless Biotech Co., Ltd. Other raw materials are all commonly used raw materials in this field and are purchased through commercial channels.

The recombinant collagen involved in the following content is the product prepared in the preparation example. The pET-28a(+) vector in the preparation example was purchased from McLean; the BL21 (DE3) chemically competent cells were purchased from Merck. The product number is Product of CMC0014-4X40UL.

Preparation example

This preparation example provides a recombinant collagen freeze-dried powder, and its preparation method is carried out with reference to the method disclosed in preparation example 1 in ZL202310123476.3, as follows:

(1) Recombinant plasmid construction: According to the nucleotide sequence shown in SEQ ID NO: 1, select the pET-28a(+) vector suitable for the expression vector E. coli host, and send it to a sequencing company for synthesis to obtain recombinant collagen expression Vector pET-28a-1.

(2) Construction of recombinant fermentation strain: Use chemical transformation method to transfer the pET-28a-1 expression vector into Escherichia coli (BL21 (DE3)) competent cells, and use agarose gel electrophoresis to screen the positive clones in the transformed plate colonies. Transfer the selected positive clones to 2 mL LB medium, culture at 37°C, 200 rpm for 12 hours, dilute the cultured bacterial liquid 1 time with 50% glycerol, aliquot into cryovials, and freeze Under the condition of -80℃, a recombinant collagen fermentation strain was obtained.

(3) Fermentation culture of recombinant fermentation strain: Spread 10 μL of recombinant collagen fermentation strain on a solid LB plate, culture it in a 37°C incubator for 12 hours, select a single clone, and inoculate it into 50 mL of LB containing 50 μg/mL kanamycin. In the liquid culture medium, place it in a shaker at 37°C and culture at 200 rpm for 14 hours, then inoculate 200 mL of LB liquid culture medium containing 50 μg/mL kanamycin at a ratio of 1:100, and culture at 37°C until the bacterial solution OD= After 0.6, add IPTG to reach a final concentration of 1mM, continue culturing for 4 hours, and then centrifuge at 4°C, 4000rpm for 15 minutes to collect the cells.

(4) Purification of recombinant collagen: Ultrasonically disrupt the cells and centrifuge, wash the precipitate with TritonX-100 washing solution, centrifuge again to remove the supernatant, add 6M guanidine hydrochloride to the precipitate, and adjust the pH to 8. After fully dissolving the precipitate, add the solution Pass through nickel affinity chromatography resin, and use 20mM, 50mM, 100mM, 200mM, 500mM concentration gradient imidazole solution to elute in sequence. After the eluent is concentrated using a protein concentration column, the concentrated solution is collected and dialyzed overnight (dialysis bag, RC membrane, 14KD, Flat width 44mm (Sangon F600112)), then use the BCA kit to detect the protein concentration, and add 0.5mL of mannitol aqueous solution with a volume concentration of 5% per 5mg of protein and mix it with the mannitol aqueous solution, and then use a freeze dryer to Pre-freeze at -40°C for 10 minutes, and finally freeze-dry at -35°C to obtain recombinant collagen freeze-dried powder.

Example 1

This embodiment provides a skin barrier repair composition, which is composed of the following components in terms of parts by mass: 0.1 part of recombinant collagen, 0.5 part of conopeptide, 0.3 part of Schizophyllan, and 0.2 part of ectoin. All components can be physically mixed evenly according to mass ratio.

Example 2

This embodiment provides a skin barrier repair composition, which is composed of the following components in terms of parts by mass: 0.05 parts of recombinant collagen, 1 part of conopeptide, 0.1 part of Schizophyllan, and 0.5 part of ectoin. All components can be physically mixed evenly according to mass ratio.

Example 3

This embodiment provides a skin barrier repair composition, which is composed of the following components in terms of parts by mass: 0.1 part of recombinant collagen, 0.2 part of conopeptide, 0.5 part of Schizophyllan, and 0.2 part of ectoin. All components can be physically mixed evenly according to mass ratio.

Comparative example 1

This comparative example provides a composition, which differs from Example 1 only in that it does not contain recombinant collagen, and its reduced mass is proportionally distributed to the mass of conopeptide, schizophyllan and ectoin, Other components and contents remain unchanged. All components can be physically mixed evenly according to mass ratio.

Comparative example 2

This comparative example provides a composition, which differs from Example 1 only in that it does not contain conopeptide, and its reduced mass is proportionally distributed to the mass of recombinant collagen, Schizophyllan and ectoin, Other components and contents remain unchanged. All components can be physically mixed evenly according to mass ratio.

Comparative example 3

This comparative example provides a composition, which differs from Example 1 only in that it does not contain Schizophyllan, and its reduced mass is proportionally distributed to the mass of conopeptide, recombinant collagen and ectoin, Other components and contents remain unchanged. All components can be physically mixed evenly according to mass ratio.

Comparative example 4

This comparative example provides a composition, which differs from Example 1 only in that it does not contain ectoine, and its reduced mass is proportionally distributed to the mass of conopeptide, Schizophyllan and recombinant collagen, Other components and contents remain unchanged. All components can be physically mixed evenly according to mass ratio.

Application example 1

This application example provides an emulsion that strengthens skin barrier repair. Its formula includes the following ingredients:

Its preparation method is:

(1) Mix and homogenize water and the skin barrier repair composition at 30°C to obtain a water phase; mix and homogenize the oil phase and emulsifier at 40°C to obtain an oil phase;

(2) Mix and homogenize the water phase, the oil phase and the remaining preparation raw materials at 40°C to obtain the emulsion that strengthens skin barrier repair.

Application Example 2-3

This application example provides two emulsions that strengthen skin barrier repair. The only difference between their formulas and Application Example 1 is that the skin barrier repair composition of Example 1 is replaced with an equal amount of the skin barrier repair composition prepared in Examples 2-3. substance, other components and contents remain unchanged. The preparation method is as shown in Application Example 1.

Application example 4

This application example provides an emulsion that strengthens skin barrier repair. The only difference between its formula and application example 1 is the lack of white pond flower seed oil. The reduced mass is allocated to the mass of sweet almond oil and grape seed oil in proportion. The other The ingredients and content remain unchanged. The preparation method is as shown in Application Example 1.

Application example 5

This application example provides an emulsion that strengthens skin barrier repair. The only difference between its formula and application example 1 is the lack of sweet almond oil. The reduced mass is allocated to the mass of white pond flower seed oil and grape seed oil in proportion. The other The ingredients and content remain unchanged. The preparation method is as shown in Application Example 1.

Application example 6

This application example provides an emulsion that strengthens skin barrier repair. The only difference between its formula and application example 1 is the lack of grape seed oil. The reduced mass is allocated to the mass of white pond flower seed oil and sweet almond oil in proportion. The other The ingredients and content remain unchanged. The preparation method is as shown in Application Example 1.

Comparative application examples 1-4

Comparative Application Examples 1-4 provide four emulsions that strengthen skin barrier repair. The only difference between their formulas and Application Example 1 is that the skin barrier repair composition of Example 1 is replaced with an equal amount of skin prepared by Comparative Examples 1-4. Barrier repair composition, other components and contents remain unchanged. The preparation method is as shown in Application Example 1.

Comparative application example 5

Comparative Application Example 5 provides an emulsion. The only difference between its formula and Application Example 1 is that it lacks the skin barrier repair composition. Its reduced mass is made up by water, and other components and contents remain unchanged.

Application example 7

This application example provides an emulsion that strengthens skin barrier repair. Its formula includes the following ingredients:

Its preparation method is:

(1) Mix and homogenize water and the skin barrier repair composition at 30°C to obtain a water phase; mix and homogenize the oil phase and emulsifier at 40°C to obtain an oil phase;

(2) Mix and homogenize the water phase and oil phase with the plant-derived conditioning agent and the remaining preparation raw materials at 40°C to obtain the emulsion that strengthens skin barrier repair.

Application example 8

This application example provides an emulsion that strengthens skin barrier repair. The only difference between its formula and application example 4 is the lack of witch hazel extract. The mass percentage of breviscapus extract is 8%, and other components and contents remain the same. Change.

Application example 9

This application example provides an emulsion that strengthens skin barrier repair. The only difference between its formula and application example 4 is the lack of breviscapus extract. The mass percentage of witch hazel extract is 8%. The other components and contents remain the same. Change.

Test example 1

Safety evaluation:

Take the products prepared in Application Examples 1-9 and Comparative Application Examples 1-5 and place them in a patch tester. Place the patch tester containing the test substance on the flexor side of the forearm of the subject (20 people), and gently use the palm of your hand to Press to apply it evenly on the skin for 24 hours; observe skin irritation and sensitization according to Table 1 30 minutes, 24 hours, and 48 hours after removing the test substance spot tester, and record the observation results.

Table 1

The results show that the scores of the products prepared in Application Examples 1-9 and Comparative Application Examples 1-5 are both 0, which means that the skin barrier repair composition involved in the present invention and the emulsion prepared therefrom are safe and non-irritating to the skin.

Test example 2

Moisturizing effect evaluation:

The instrument is a German Courage+Khazaka electronic model: Derma UnitSSC. The test temperature is 25°C and the humidity is 60%. Select 150 volunteers aged 18-40 with healthy skin, half of them male and female, and randomly divide them into 15 groups, with 5 males and 5 females in each group, and use the products provided in Application Examples 1-9 and Comparative Application Examples 1-5 respectively. , the blank group does not use any products. Test the skin moisture content of each subject before use and after 5 hours of use (calculated as 100% before use). Each data is tested three times. After taking the average, calculate the average value of each group, retaining one decimal place. . The results are shown in Table 2.

Table 2

It can be seen from the data in Table 2 that the composition involved in the present invention and the cosmetics prepared therefrom can repair the skin barrier, prevent water evaporation, help strengthen and restore the skin's moisturizing state, and recombinant collagen, conotide, Schizophyllan and Ecdoine has a significant synergistic effect on the above-mentioned effects, and the addition of Erigeron extract and witch hazel extract can further enhance the above-mentioned effects of the product.

Test example 3

Anti-allergic performance evaluation:

The hyaluronidase in vitro inhibition test was performed using the Elson-Morgan method. The sample solution group is to take 0.1 mL of 0.25 mmol/L CaCl ₂ solution and 0.5 mL of hyaluronidase solution and incubate them at 37°C for 20 min; add 0.5 mL of 10% diluted aqueous solution of the products of Examples 1-3 and Comparative Examples 1-4 respectively. , continue to incubate at 37℃ for 20min; add 0.5mL sodium hyaluronate solution and incubate at 37℃ for 30min, and leave it at room temperature for 5min; add 0.1mL0.4mol/L NaOH solution and 0.5mL acetylacetone solution, place it in a boiling water bath and heat for 15min immediately. Cool with ice water for 5 minutes; add 1.0 mL of Ehrlich's reagent and dilute it with 3.0 mL of absolute ethanol, leave it for 20 minutes to develop color, and measure the absorbance value with a spectrophotometer. Calculation formula: Hyaluronidase inhibition rate (%) = [((AB)-(CD))/AB]×100%, where A represents (hyaluronidase + deionized water + sodium hyaluronate) The OD value of the control solution, B represents the OD value of the (acetate buffer + deionized water + acetate buffer) control blank solution, C represents the OD value of the (hyaluronidase + sample + sodium hyaluronate) sample solution, D represents the OD value of the sample blank solution (acetate buffer + sample + acetate buffer).

Each group of samples was tested in parallel three times. After taking the average, calculate the average value of each group and keep it to one decimal place. The results are shown in Table 3.

table 3

Group Hyaluronidase inhibition (%) Example 1 68.4 Example 2 66.7 Example 3 63.9 Comparative example 1 66.8 Comparative example 2 68.0 Comparative example 3 57.3 Comparative example 4 51.2

It can be seen from the data in Table 3 that the composition involved in the present invention has excellent hyaluronidase inhibition effect, can soothe the skin's inflammatory response and other uncomfortable symptoms, maintain the stability and health of the skin, and recombinant collagen and conotase peptides , Schizophyllan and Ectoin have significant synergistic effects on the above-mentioned effects.

Test example 4

Anti-aging effect evaluation:

The instrument is the German Courage+Khazaka skin elasticity tester MPA-580, with a test temperature of 25°C and a humidity of 60%. 150 volunteers aged 18-40 with healthy skin were selected, half of them male and female, and randomly divided into 15 groups, with 5 males and 5 females in each group. The measurement area was marked on the inside of the forearm of both hands of the subjects, and the experimental area was at least 3cm× 3cm, with at least 1cm between each test area. The products provided in Application Examples 1-9 and Comparative Application Examples 1-5 were used respectively, and the control group did not use any products. 14 groups of test samples were coated in a single time at a dosage of 2ml/ ^cm2 (the blank group was pure water). Use latex gloves to apply the sample evenly to the test area and pat gently until absorbed. Use once a day for two weeks and measure the results. Touch the probe vertically to the area to be measured to start the test, and remove the probe until the test cycle is completed. Take three points for each area to be measured. After taking the average, calculate the average value of each group. The results are shown in Table 4.

Table 4

It can be seen from the data in Table 4 that the composition involved in the present invention and the cosmetics prepared therefrom can repair the skin barrier, prevent water evaporation, help strengthen and restore the skin's moisturizing state, and recombinant collagen, conotide, Schizophyllan and Ecdoine has a significant synergistic effect on the above-mentioned effects, and the addition of Erigeron extract and witch hazel extract can further enhance the above-mentioned effects of the product.

The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent replacement of raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present invention, a variety of simple modifications can be made to the technical solutions of the present invention. These simple modifications All belong to the protection scope of the present invention.

In addition, it should be noted that each of the specific technical features described in the above-mentioned specific embodiments can be combined in any suitable manner without conflict. In order to avoid unnecessary repetition, the present invention combines various possible combinations. The combination method will not be further explained.

Claims (10)

1. A skin barrier repair composition comprising recombinant collagen, wherein the skin barrier repair composition comprises recombinant collagen, conopeptides, schizophyllan and ectofactor.

2. The skin barrier repair composition containing recombinant collagen according to claim 1, wherein the skin barrier repair composition comprises, in parts by mass, 0.01-2 parts of recombinant collagen, 0.1-3 parts of conopeptide, 0.1-0.5 part of schizophyllan and 0.1-1 part of ectoin.

3. The skin barrier repair composition containing recombinant collagen according to claim 1 or 2, wherein the nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID No. 1.

4. Use of the skin barrier repair composition containing recombinant collagen according to any one of claims 1 to 3 in the preparation of a cosmetic.

5. The use according to claim 4, wherein the skin barrier repair composition comprising recombinant collagen is present in the cosmetic in an amount of 0.1 to 15% by mass.

6. An emulsion for strengthening skin barrier repair is characterized by comprising the following preparation raw materials in percentage by mass: the skin barrier repair composition containing recombinant collagen according to any one of claims 1 to 3, 0.1 to 15%, an oil phase 1 to 20%, an emulsifier 1 to 10% and water 50 to 90%.

7. The skin barrier repair enhancing emulsion of claim 6, wherein the oil phase comprises any one or a combination of at least two of glycerol caprylate, squalane, olive oil, white pool seed oil, jojoba oil, shea butter, sweet almond oil, grape seed oil, caprylic/capric triglyceride, hydrogenated cocoglyceride, phytosterol isostearate, isononyl isononanoate, arachidyl alcohol, vitamin a, vitamin E, or vitamin F; preferably, a combination of white pool flower seed oil, sweet almond oil, grape seed oil and vitamin E;

preferably, the emulsifier comprises any one or a combination of at least two of glycerol stearate, cetostearyl alcohol, sodium stearoyl lactylate, glycerol stearate citrate, cholesterol, cetostearyl alcohol oleyl oleate, sorbitan stearate, polyglycerol-10 oleate or polysorbate-60.

8. The skin barrier repair enhancing emulsion according to claim 6 or 7, wherein the emulsion is prepared from the following raw materials in mass percent: 1-15% of plant source conditioning agent, 0.1-2% of pH regulator, 0.01-0.5% of chelating agent, 0.05-0.5% of antioxidant and 0.1-0.5% of aromatic agent or the combination of at least two.

9. The emulsion for enhancing skin barrier repair of claim 8, wherein the plant-derived conditioning agent comprises witch hazel extract and/or erigeron breviscapus extract.

10. A method of preparing an emulsion for enhancing skin barrier repair according to any one of claims 6 to 9, wherein the method of preparing comprises the steps of:

(1) Mixing water and skin barrier repair composition at 25-40deg.C, homogenizing to obtain water phase; mixing the oil phase with emulsifier at 35-50deg.C, and homogenizing to obtain oil phase;

(2) Mixing and homogenizing the water phase, the oil phase and the rest preparation raw materials at 25-40 ℃ to obtain the emulsion for reinforcing skin barrier repair.