CN117569013A - Micro-nano fiber membrane containing collagen fibers and preparation method and application thereof - Google Patents

Abstract

The invention belongs to the technical field of biomedical materials, and relates to a micro-nano fiber membrane containing collagen fibers, a preparation method and application thereof. The invention provides a preparation method of a micro-nanofiber membrane containing collagen fibers, which comprises the steps of carrying out high-speed wall breaking treatment on a acellular dermal matrix to prepare a collagen fiber spinning solution, and preparing the micro-nanofiber membrane containing collagen fibers by an electrostatic spinning method. According to the invention, the acellular dermal matrix is dissolved by using the mixed solution of chloride, water, acetic acid and ethanol, so that collagen can be quickly dissolved, collagen peptide chains are not easy to damage, the four-level structure of collagen fibers is maintained, the electrospinning property of the solution is improved, the viscosity and the moldability of the solution are improved, the morphology of the collagen micro-nano fiber membrane is further improved, the original collagen natural structure is kept, and the collagen micro-nano fiber membrane has biocompatibility, degradability and mechanical strength and can be used in the field of tissue wound repair. Compared with the traditional collagen micro-nanofiber membrane, the collagen micro-nanofiber membrane provided by the invention has the advantage that the mechanical strength is remarkably improved.

Description

A kind of micro-nano fiber membrane containing collagen fibers and its preparation method and application

Technical field

The invention belongs to the technical field of biomedical materials and relates to a micro-nano fiber membrane containing collagen fibers and its preparation method and application.

Background technique

Collagen is widely found in animal tissues and organs, especially in vertebrates. Collagen accounts for 30% of all protein content. Type I collagen has the characteristics of absorbable degradation, biocompatibility, and promotion of tissue repair and regeneration in the body. , collagen is used as an ideal biomedical material and is widely used in biomedical fields such as tissue engineering and wound repair.

Wound repair of burns or skin tissue defects is often done with autologous or artificial skin, but it is easy to form varying degrees of scar hyperplasia and contracture deformity. In recent years, acellular dermal matrix, as a natural extracellular matrix with low immunogenicity, removes cells As a component, the retained macromolecules such as collagen, elastin, and proteoglycans can form a complex 3D structural network and are widely used in the field of tissue wound repair.

The diameter of nanofibers prepared by electrospinning is usually between 10nm and 10μm, and its porosity and interstitial connectivity rate are very high, which can be used to block wound infections caused by bacteria. In addition, electrospinning can retain embedded materials to the greatest extent biological activity and improve its bioavailability.

CN114366844B discloses a composite hemostatic membrane material made of acellular dermal matrix as a material, enzymatically hydrolyzed by acidic complex enzyme HD-1, mixed with notoginseng and chitosan to obtain a spinning solution, and electrospun to obtain a composite hemostatic membrane material. The obtained collagen no longer has a quaternary structure - collagen fiber structure, but is mostly molecular collagen, such as collagen, gelatin, etc., which are all hydrolyzed products of collagen aggregates and are prepared through electrospinning technology. The collagen molecules lack cross-linking, resulting in insufficient mechanical strength and biocompatibility issues. And the electrospinning solution of collagen materials mostly uses hexafluoroisopropanol (HFIP) as the solvent. The 202310919679.3 patent discloses using dermal decellularized extracellular matrix as the material, using hexafluoroisopropanol (HFIP) as the solvent, and passing the electrospinning Composite artificial skin is prepared from silk. The selected HFIP is a highly polar solvent. While it dissolves collagen, it will destroy the triple helix structure of collagen itself, affecting its biological activity, and even turning it into gelatin. , and the problem of insufficient mechanical strength. In addition, HFIP itself has the characteristics of high cost, high toxicity and high volatility, which will damage the health of operators and pollute the environment during use. It can be seen that due to the influence of its collagen material structure, the micro-nano fiber membrane obtained through electrospinning technology still has certain problems in terms of mechanical strength and safety.

Contents of the invention

The object of the present invention is to provide a tissue repair collagen micro-nano fiber membrane with good air permeability, can block wound infection, good mechanical strength and low immunogenicity and a preparation method thereof. The present invention mixes acellular dermal matrix with saline, acetic acid and ethanol to obtain a collagen fiber spinning liquid. The morphology of the nanofiber membrane is changed through electrospinning so that it retains the original natural structure of collagen and is biocompatible and reproducible. The degradability and mechanical strength can be used in the field of tissue wound repair. In addition, compared with the traditional collagen micro-nano fiber membrane, the mechanical strength of the collagen micro-nano fiber membrane of the present invention has been significantly improved.

Based on the above objectives, the present invention solves this need in the field by providing a micro-nano fiber membrane containing collagen fibers and a preparation method thereof.

On the one hand, the present invention relates to a method for preparing a micro-nano fiber membrane containing collagen fibers. The method for preparing a micro-nano fiber membrane containing collagen fibers includes: subjecting acellular dermal matrix to high-speed wall-breaking treatment to prepare collagen. Fiber spinning liquid, electrostatic spinning to obtain the micro-nano fiber membrane containing collagen fibers; the acellular dermal matrix is subjected to high-speed wall breaking treatment at a rotation speed of 25000rpm/min-30000rpm/min, with an interval of 10min-20min.

Further, in the method for preparing a micro-nano fiber membrane containing collagen fibers provided by the present invention, the acellular dermal matrix is a heterogeneous acellular dermal matrix.

Further, in the preparation method of the micro-nano fiber membrane containing collagen fibers provided by the present invention, the preparation method of the collagen fiber spinning liquid is: the acellular dermal matrix is dissolved in the mixed solution to prepare the collagen fiber spinning liquid ; The mixed solution is composed of chloride salt, acid, alcohol and water; in the mixed solution including chloride salt, acid, alcohol and water, a collagen fiber spinning liquid is obtained.

Further, in the preparation method of a collagen fiber-containing micro-nano fiber membrane provided by the present invention, the chloride salt is sodium chloride; the water is pure water; the acid is acetic acid; and the alcohol is ethanol.

Further, in the preparation method of a micro-nano fiber membrane containing collagen fibers provided by the present invention, the mass fraction of collagen fibers in the collagen fiber spinning liquid is 5%-10%; The mass fraction of chloride salt is 0.5%-2.0%; the mass fraction of water in the collagen fiber spinning liquid is 13.20%-23.63%; the mass fraction of acid in the collagen fiber spinning liquid is 47.25%-61.60%; The mass fraction of alcohol in the collagen fiber spinning liquid is 13.2%-23.63%.

Further, in the preparation method of a micro-nano fiber membrane containing collagen fibers provided by the invention, the electrospinning parameters include: 0.5mL/h-1.0mL/h, the spinning time is 5h-10h, and the voltage is 25kV-30kV, receiving distance is 15cm-20cm.

Further, in the preparation method of a micro-nano fiber membrane containing collagen fibers provided by the invention, the ambient temperature of the electrospinning is 22°C-28°C, and the ambient humidity of the electrospinning is 30%-40 %.

Further, in the preparation method of a micro-nano fiber membrane containing collagen fibers provided by the present invention, the electrospinning receiver adopts a roller receiver, and the rotation speed of the roller receiver is 300r/min～600r/min. .

On the other hand, the present invention relates to a micro-nano fiber membrane, which is prepared by using the method for preparing a collagen fiber-containing micro-nano fiber membrane described in any one of the above.

On the other hand, the present invention relates to the application of a collagen fiber-containing micro-nano fiber membrane according to any one of the above in the preparation of wound repair drugs.

Compared with the prior art, the present invention has the following beneficial effects or advantages:

The present invention uses acellular dermal matrix as raw material, uses a mixed solution of water, acetic acid, and ethanol as a solvent, and adds chlorine salt to it to prepare a collagen fiber-containing spinning solution, and electrospinning produces a collagen micro-nano fiber membrane with tissue repair. , the obtained fiber membrane material has good mechanical properties, biocompatibility and biodegradability. At the same time, the electrospinning process used in the present invention has simple production equipment and easy operation, and does not require additional post-processing processes. Compared with the traditional collagen fiber membrane, the tissue repair fiber membrane of the present invention has significantly improved mechanical strength and can effectively prevent wound infection. In particular, the tissue wound repair efficiency is good and has low immunity and safety. Strong, it provides theoretical and experimental basis for electrospinning to prepare collagen fiber membranes derived from acellular dermal matrix, which can be applied in the field of biomedical materials technology.

Description of the drawings

In order to explain the embodiments of the present invention or the technical solutions in the prior art more clearly, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are only are some embodiments of the invention.

Figure 1 is a scanning electron microscope picture and a fiber diameter distribution picture of a micro-nano fiber membrane containing collagen fibers prepared in an embodiment of the present invention. A is a scanning electron microscope picture of the micro-nano fiber membrane containing collagen fibers prepared in the embodiment of the present invention; B is the diameter distribution picture of the micro-nano fiber membrane containing collagen fibers prepared in the embodiment of the present invention.

Figure 2 is a scanning electron microscope picture and a fiber diameter distribution picture of the collagen fiber membrane prepared in the comparative example of the present invention. A is a scanning electron microscope picture of the micro-nano fiber membrane containing collagen fibers prepared in the comparative example of the present invention; B is a diameter distribution picture of the micro-nano fiber membrane containing collagen fibers prepared in the comparative example of the present invention.

Figure 3 is a physical diagram of a micro-nano fiber membrane containing collagen fibers prepared in an embodiment of the present invention.

Figure 4 is a physical diagram of the collagen fiber membrane prepared in the comparative example of the present invention.

Figure 5 is an infrared spectrum of the micro-nano fiber membrane prepared in the embodiment of the present invention and the collagen fiber membrane prepared in the comparative example. Ag-col represents the infrared spectrum of the collagen fiber-containing micro-nano fiber membrane prepared in the embodiment of the present invention, col represents the infrared spectrum of the collagen fiber membrane of the comparative example of the present invention; wherein Amide A-amide A band absorption peak, Amide B-amide B band absorption peak, Amide I-amide I band absorption peak, Amide II-amide II band absorption peak, Amide III-amide III band absorption peak.

Figure 6 is a mechanical stretching diagram of the micro-nano fiber membrane prepared in the embodiment of the present invention and the collagen fiber membrane prepared in the comparative example.

Figure 7 is a wound healing diagram of the micro-nano fiber membrane prepared in the embodiment of the present invention and the collagen fiber membrane prepared in the comparative example.

Figure 8 is a histological Masson and H&E staining diagram of the micro-nano fiber membrane prepared in the embodiment of the present invention and the collagen fiber membrane prepared in the comparative example.

Detailed ways

The technical solutions of the present invention will be described below with reference to examples. However, the present invention is not limited to the following examples. The experimental methods and detection methods described in each example are conventional methods unless otherwise specified. The equipment and raw materials mentioned above can be purchased in the market unless otherwise specified.

Example 1

This embodiment provides a method for preparing a micro-nano fiber membrane containing collagen fibers, which includes the following steps:

Step 1: Cut 5g of freeze-dried xenogeneic acellular dermal matrix (made in the laboratory) into small pieces with scissors, place it in 23.42ml water (mass fraction 23.625%), 45ml acetic acid (mass fraction 47.25%), 29.94 ml (mass fraction 23.625%) of ethanol, then add 0.5g sodium chloride (mass fraction 0.5%) and soak for 3 hours. When the solution becomes transparent, a mixture of 5% collagen fibers is obtained;

Step 2: Place the collagen fiber mixture obtained in step 1 in a high-speed wall breaking machine, and crush it at intervals of 30 minutes until a transparent colloidal solution is obtained, which is a 5% collagen fiber spinning solution;

Step 3: Add the spinning liquid to the syringe of the electrospinning system, install the syringe on the micro-injection pump, and set the electrospinning process parameters: voltage is 25kV, spinning liquid flow rate is 0.5mL/h, and receiving distance is 15cm , the spinning time is 5 hours. During the spinning process, the ambient temperature is controlled to 22°C, the ambient humidity is 30%, and the rotation speed of the drum receiver is 300r/min. Then the electrospinning experiment is started. After the spinning is completed, it is vacuum dried at 40°C. In 2 hours, the tissue repair fiber membrane containing collagen fibers was obtained.

Example 2

This embodiment provides a method for preparing a micro-nano fiber membrane containing collagen fibers, which includes the following steps:

Step 1: Cut 8g of freeze-dried xenogeneic acellular dermal matrix (made in the laboratory) into small pieces with scissors, and place it in 18.16ml water (mass fraction 18.16%), 51.89ml acetic acid (mass fraction 54.48%), Add 1.2g sodium chloride (mass fraction 1.2%) to a mixed solution composed of 23.02ml (mass fraction 18.16%) ethanol, and soak for 3 hours. When the solution becomes transparent, a mixed solution of 8% collagen fiber is obtained;

Step 2: Place the collagen fiber mixture obtained in step 1 into a high-speed wall breaking machine and stir for 30 minutes at intervals until a transparent colloidal solution is obtained, which is the 8% collagen fiber spinning solution;

Step 3: Add the spinning liquid to the syringe of the electrospinning system, install the syringe on the micro-injection pump, and set the electrospinning process parameters: voltage is 27kV, spinning liquid flow rate is 0.8mL/h, and receiving distance is 18cm , the spinning time is 10h, during the spinning process, the ambient temperature is controlled to 25°C, the ambient humidity is 35%, the rotation speed of the drum receiver is 500r/min, and then the electrospinning experiment is started, and after the spinning is completed, it is vacuum dried at 40°C In 2 hours, the tissue repair fiber membrane containing collagen fibers was obtained.

Example 3

This embodiment provides a method for preparing a micro-nano fiber membrane containing collagen fibers, which includes the following steps:

Step 1: Cut 10g of freeze-dried xenogeneic acellular dermal matrix (made in the laboratory) into small pieces with scissors, and place it in 13.20ml water (mass fraction 13.20%), 58.67ml acetic acid (mass fraction 61.60%), Add 2.0g sodium chloride (mass fraction 2.0%) to a mixed solution composed of 16.92ml (mass fraction 13.20%) ethanol, and soak for 3 hours. When the solution becomes transparent, a mixed solution of 10% collagen fiber is obtained;

Step 2: Place the collagen fiber mixture obtained in step 1 into a high-speed wall breaking machine and stir for 30 minutes at intervals until a transparent colloidal solution is obtained, which is the 10% collagen fiber spinning solution;

Step 3: Add the spinning liquid to the syringe of the electrospinning system, install the syringe on the micro-injection pump, and set the electrospinning process parameters: voltage is 30kV, spinning liquid flow rate is 1.0mL/h, and receiving distance is 20cm , the spinning time is 8 hours, during the spinning process, the ambient temperature is controlled to 28°C, the ambient humidity is 40%, and the rotation speed of the drum receiver is 600r/min; then the electrospinning experiment is started, and after the spinning is completed, it is vacuum dried at 40°C In 2 hours, the tissue repair fiber membrane containing collagen fibers was obtained.

Comparative example 1

This comparative example is intended to illustrate the preparation of traditional molecular collagen through electrospinning technology by comparing the mechanical strength of the fiber membrane prepared by traditional collagen electrospinning with the collagen fiber membrane prepared by using acellular dermal matrix as raw material in the present invention. The obtained collagen molecules lack cross-linking and have insufficient mechanical strength. This comparative example provides a method for preparing a fibrous membrane containing traditional collagen, which includes the following steps:

Step 1: Place 5g of collagen (purchased from Shanghai McLean) into a mixture of 18.90ml water (mass fraction 18.90%), 54.00ml acetic acid (mass fraction 56.70%), and 23.95ml ethanol (mass fraction 18.90%). To the mixed solution, add 0.5g sodium chloride (mass fraction 0.5%) and soak for 3 hours. When the solution becomes transparent, a 5% collagen mixed solution is obtained;

Step 2: Place the collagen mixture obtained in step 1 into a high-speed wall breaking machine and stir for 30 minutes at intervals until a transparent colloidal solution is obtained, which is a 5% collagen fiber spinning solution;

Step 3: Add the spinning liquid to the syringe of the electrospinning system, install the syringe on the micro-injection pump, and set the electrospinning process parameters: voltage is 25kV, spinning liquid flow rate is 0.5mL/h, and receiving distance is 15cm , the spinning time is 5 hours. During the spinning process, the ambient temperature is controlled to 22°C, the ambient humidity is 30%, and the rotation speed of the drum receiver is 300r/min. Then the electrospinning experiment is started. After the spinning is completed, it is vacuum dried at 40°C. After 2 hours, a fibrous membrane containing traditional collagen was obtained.

Example 4

This example provides a scanning electron microscope test of a micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 and a fiber membrane prepared with traditional collagen in Comparative Example 1.

Scanning electron microscope test method:

A micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 of the present invention and a fiber membrane electrospun from traditional collagen as raw material in Comparative Example 1 were subjected to scanning electron microscopy tests, and the working distance (WD) was set to 10.6 mm. The porous structure was observed. The scanning electron microscope results and fiber diameter distribution results of the micro-nano fiber membrane containing collagen fibers prepared in the Example are shown in Figure 1. The physical picture of the micro-nano fiber membrane containing collagen fibers prepared in the Example is shown in Figure 3. shown. The scanning electron microscope results and fiber diameter results of the fiber membrane of Comparative Example 1 are shown in Figure 2, and the physical picture of the fiber membrane of Comparative Example 1 is shown in Figure 4. It can be seen from Figure 1 that the porosity of the fiber membrane of the example was measured to be 65.63% and the diameter was 0.186 μm. As can be seen from Figure 2, the porosity of the fiber membrane of the comparative example was measured to be 71.03%, and the diameter was 0.113 μm. It can be seen from the comparison of the electron microscope scanning results of the two that the results of a micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 of the present invention and the fiber membrane prepared in the comparative example are intended to illustrate that increasing the molecular weight of the electrospinning solution can increase the size of the fiber. Diameter, the increase in fiber diameter can increase the mutual contact area between fibers, increase the friction between fibers, and improve the strength of the fiber membrane to a certain extent. It can be seen from the results of Figure 3 and Figure 4 that the comparison between the two illustrates that as the fiber diameter increases, the surface defects of the fiber membrane decrease. The micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 has fewer surface defects and can be effectively avoided. Stress concentration phenomenon.

Example 5

This example provides an infrared detection test of a micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 and a fiber membrane electrospun in Comparative Example 1 using traditional collagen as raw material.

Infrared detection method:

A micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 of the present invention and a fiber membrane electrospun from traditional collagen as raw material in Comparative Example 1 were subjected to infrared detection tests, using the potassium bromide (KBr) tableting method. For analysis, take 100 mg of KBr crystals, mix them with 2 mg of Ag-col and Col, grind and press them into tablets. At room temperature (24±1°C) and in a dry environment (relative humidity less than 65%), the wave number range is 4000cm ^-1 -400cm ^-1 Scan 32 times to take the average, and the resolution is set to 4cm ^-1 . The infrared detection result images of the examples and comparative examples are shown in Figure 5. It can be seen from Figure 5 that the infrared absorption peaks of the amide I band of Ag-col and Col appear at 1662cm ^-1 and 1657cm ^-1 respectively; the absorption peaks of the amide II band are concentrated at 1559cm ^-1 and 1544cm ^-1 respectively; and The absorption peaks of the amide III band all appear at a wave number of 1241cm ^-1 ; the absorption peaks of the amide A band appear at 3538cm ^-1 and 3436cm ^-1 respectively; the characteristic absorption peaks of the amide B band appear at 3053cm ^-1 and 3049cm ^- respectively. ¹ place. From the comparison of the infrared test results of the two, it can be seen that the comparison of the results of a micro-nano fiber membrane containing collagen fibers prepared by the present invention and the fiber membrane prepared by the comparative example shows that the characteristic absorption peaks of the amide band of both are obviously present, and there is no difference. Large, confirming that the basic secondary structures of the two materials are similar, and the natural triple helix structure remains intact.

Example 6

This example provides a mechanical tensile test of a micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 and a fiber membrane electrospun in Comparative Example 1 using traditional collagen as raw material.

Mechanical tensile test method:

A kind of micro-nano fiber membrane containing collagen fiber prepared in the embodiment of the present invention and Comparative Example 1. A fiber membrane electrospun with traditional collagen as raw material was subjected to a mechanical tensile test. A constant speed stretching method was used, and a medium dumbbell style was selected. Die cutter, set the servo material multifunctional high and low temperature control testing machine to 20mm/min constant speed stretching, the air conditioning temperature is 23±2℃, and the relative humidity is 50±5℃. The infrared detection result images of Examples 1-3 and Comparative Example 1 are shown in Figure 6. It can be seen from Figure 6 that the mechanical strength of Examples 1-3 in the test group is higher than that of Comparative Example 1. By calculating the tensile strength, the tensile strengths of the micro-nanofiber membranes containing collagen fibers prepared in Examples 1-3 can be obtained, respectively. are 48.76kPa, 51.32kPa, and 46.42kPa. The tensile strength of the fiber membrane prepared by electrospinning of collagen as raw material in Comparative Example 1 is 37.73kPa. It can be seen from the comparison of the mechanical tensile test results of the two that the results of a micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 of the present invention and the fiber membrane prepared in Comparative Example 1 are intended to illustrate that the collagen fiber-containing micro-nano fiber membrane prepared in Examples 1-3 of the present invention is The micro-nano fiber membrane of collagen fibers has higher mechanical properties.

Example 7

This example provides a skin sensitization test and a wound tissue repair test of a micro-nano fiber membrane containing collagen fibers prepared in Examples 1-3 and a fiber membrane electrospun from traditional collagen as raw material in Comparative Example 1.

(1) Skin sensitization test

A sensitization test was conducted according to GB/T 16886.10-2005/ISO 10993-10:2002. The back hair of 4 healthy white mice was removed, and 0.5g of the fiber membrane prepared in Examples 1-3 and Comparative Example 1 was placed on the left side of the spine. For skin, use normal saline as the control group. Apply it to the skin on the right side of the spine. Cover the contact site with gauze and fix the patch with a bandage for at least 4 hours. Record the conditions of each contact site at 1h, 24h, 48h, and 72h after removing the patch. The results of the sensitization test can be observed with the naked eye. Compared with the control group, the skin of animals treated with 1-3 and Comparative Example 1 did not have erythema, edema and other skin reactions at 1h, 24h, 48h, and 72h. The results show that neither the micro-nano fiber membrane containing collagen fiber prepared in Examples 1-3 of the present invention nor the fiber membrane electrospun from traditional collagen as raw material in Comparative Example 1 has any skin irritation.

(2)Wound repair test

The hair on the back of the SD rat was removed, and after the skin was disinfected with iodine, a circular animal skin punch was used to create a circular full-thickness skin defect with a diameter of 8 mm on the back of the SD rat. The micro-nano fiber membranes containing collagen fibers prepared in Examples 1-3 were used as the experimental group. In Comparative Example 1, the traditional collagen electrospun fiber membranes were used as the control group. The blank group was the untreated group. After the wounds healed naturally, the wounds were treated respectively. Cover and bond treatment, and finally wrap with medical surgical film. Observe and record the wound healing status of SD rats. On the 14th day, tissue from the wound site of SD rats was cut and stained with Masson and H&E. The wound healing result images of the wounds covered by the micro-nano fiber membranes containing collagen fibers prepared in Examples 1-3, the wounds covered by the fiber membranes prepared by collagen in Comparative Example 1 and the blank group are shown in Figure 7, and histological staining images As shown in Figure 8. As can be seen from Figure 7, there was no significant difference in the wound changes in the first 3 days. On the 9th day, compared with the control group, the wound of the experimental group of the Example was significantly smaller. On the 14th day, the wound of the SD rat in the control group was not completely healed and was still red. The wounds were exposed and not covered by new skin, while the wounds in the experimental group had basically healed, with no scar tissue on the surface and no erythema and edema. As can be seen from Figure 8, the epithelial regeneration in the experimental group was more complete than that in the control group, and the collagen deposition rate in the experimental group was higher. The results show that both the micro-nano fiber membranes containing collagen fibers prepared by the present invention and the electrospun fiber membranes using traditional collagen as raw materials have a certain promoting effect on skin wound healing. The micro-nano fiber membranes containing collagen fibers prepared by the present invention The membrane has a stronger effect on promoting wound healing and is non-irritating to the skin.

As described above, the present invention can be better implemented. The above-mentioned embodiments are only descriptions of preferred embodiments of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, ordinary people in the art can Various changes and improvements made by skilled personnel to the technical solutions of the present invention should fall within the defined protection scope of the present invention.

Claims (10)

1. A method for preparing a micro-nano fiber membrane containing collagen fibers, which is characterized in that it includes: subjecting the acellular dermal matrix to high-speed wall breaking treatment, making a collagen fiber spinning liquid, and electrospinning the said micro-nano fiber membrane containing collagen fibers. Micro- and nanofiber membranes of collagen fibers; The acellular dermal matrix is subjected to high-speed wall-breaking treatment at a rotation speed of 25,000rpm/min-30,000rpm/min, with an interval of 10min-20min. 2. The method for preparing a collagen fiber-containing micro-nano fiber membrane according to claim 1, wherein the acellular dermal matrix is a heterogeneous acellular dermal matrix. 3. The preparation method of micro-nano fiber membranes containing collagen fibers according to claim 1, characterized in that, the acellular dermal matrix is subjected to high-speed wall breaking treatment and then dissolved in a mixed solution to prepare the collagen fiber spinning solution ; The mixed solution consists of chloride salt, acid, alcohol and water. 4. The method for preparing a collagen fiber-containing micro-nano fiber membrane according to claim 3, wherein the chloride salt is sodium chloride; The water is pure water; The acid is acetic acid; The alcohol is ethanol. 5. The preparation method of micro-nano fiber membranes containing collagen fibers according to claim 3, characterized in that the mass fraction of collagen fibers in the collagen fiber spinning solution is 5%-10%; The mass fraction of chloride salt in the collagen fiber spinning solution is 0.5%-2%; The mass fraction of water in the collagen fiber spinning solution is 13.20%-23.63%; The mass fraction of acid in the collagen fiber spinning solution is 47.25%-61.60%; The mass fraction of alcohol in the collagen fiber spinning liquid is 13.2%-23.63%. 6. The preparation method of collagen fiber-containing micro-nano fiber membranes according to claim 1, characterized in that the parameter settings of the electrospinning include: the spinning liquid flow rate is 0.5mL/h-1.0mL/h, The spinning time is 5h-10h, the voltage is 25kV-30kV, and the receiving distance is 15cm-20cm. 7. The preparation method of micro-nano fiber membranes containing collagen fibers according to claim 6, characterized in that the ambient temperature of the electrospinning is 22°C-28°C, and the ambient humidity of the electrospinning is 30 %-40%. 8. The preparation method of micro-nano fiber membranes containing collagen fibers according to claim 6, characterized in that the electrospinning receiver adopts a roller receiver, and the rotation speed of the roller receiver is 300r/min- 600r/min. 9. A micro-nano fiber membrane, characterized in that it is prepared by the method for preparing a collagen fiber-containing micro-nano fiber membrane according to any one of claims 1 to 8. 10. Application of the micro-nano fiber membrane according to claim 9 in the preparation of wound repair drugs.