CN117367923B - Oral cancer isolated tumor tissue specimen processing device and processing method - Google Patents

Abstract

The invention is applicable to the technical field of tissue specimen detection, and provides an oral cancer isolated tumor tissue specimen processing device and a processing method. The processing device includes a box, a partition plate, a staining chamber, a cleaning chamber, a mounting plate, and a sector gear. , rack, screen frame and moving mechanism. The present invention uses the moving mechanism to drive the partition plate to move to one side of the dip dyeing chamber. The end of the installation plate located in the dip dyeing chamber and the screen frame move downward and enter the dip dyeing solution; after the dip dyeing treatment, The moving mechanism drives the partition plate to move to one side of the cleaning chamber. The end of the installation plate located in the cleaning chamber descends into the cleaning fluid. The screen frame slides into the cleaning chamber and enters the cleaning fluid. The tumor tissue specimen is automatically and quickly completed from dip-staining to treatment. The transfer to the cleaning process does not require manual operation, which improves work efficiency, separates the dip-dyeing chamber and the cleaning chamber, and reduces the interaction between the dip-dyeing process and the cleaning process.

Description

Oral cancer isolated tumor tissue specimen processing device and processing method

Technical field

The invention belongs to the technical field of tissue specimen detection, and in particular relates to an oral cancer isolated tumor tissue specimen processing device and a processing method.

Background technique

Oral cancer is a general term for malignant tumors that occur in the oral cavity. Most of them are squamous epithelial cell carcinomas, which are so-called mucosal mutations. Oral cancer requires a pathological diagnosis process, and tumor tissue specimens need to be obtained and then processed;

After searching, the patent application with publication number CN113820193A discloses a device for processing isolated oral squamous cell carcinoma tumor tissue specimens, which includes a supporting component, a flipping component, a spray component and a soaking box. The supporting component is rotated and installed through the flipping component. In the soaking box and the supporting component is close to the mouth of the soaking box, the spray component is fixedly installed above the supporting component; the supporting component includes a cross-clamping rod, a supporting card box and a driving component. The supporting card box includes two sets of hollow shells arranged in a hemispherical shape. The two sets of hollow shells are respectively symmetrically arranged on the top of the cross clamping rod, and there are small holes in an annular equidistant array in the hollow shell;

In the above-mentioned prior art, tissue specimens are processed by setting up a soaking box, a flipping component and a spraying component. On the one hand, the entire device is in a fully open state, which is prone to cross-influence with the outside world, such as the volatilization of liquids and the entry of external dirt. , may have adverse effects. On the other hand, spraying and soaking are connected, and the spray liquid flows directly into the soaking box and is mixed. If subsequent treatment is required, the treatment effect will be affected.

Contents of the invention

The present invention provides an oral cancer isolated tumor tissue specimen processing device, aiming to solve the above problems.

The present invention is implemented as follows: an oral cancer in vitro tumor tissue specimen processing device, including:

box;

A partition plate provided in the box is used to divide the inner cavity of the box into a dyeing chamber and a cleaning chamber. A plurality of horizontal guide rods are passed through the edge of the partition plate, and the guide rods are fixedly connected to the side walls of the box. ;

A mounting plate that runs through the partition plate. The middle part of the mounting plate is rotationally connected to the partition plate through a rotating shaft. Both ends of the mounting plate are located in the dyeing chamber and the cleaning chamber respectively. One end of the rotating shaft extends to the outside of the box and is fixed with a fan. Gear, a rack is fixed on the outer wall of the box, the rack is located on the lower side of the sector gear and meshes with it, and a long slot for the movement of the rotating shaft is provided on the outer wall of the box;

A mesh frame is slidably installed on the lower surface of the installation plate and is used to place tumor tissue specimens. The partition plate is provided with a through hole for the mesh frame to pass;

A moving mechanism installed on the box to drive the partition board to move horizontally.

Preferably, sealing strips are installed on the bottom and front and rear side walls of the partition plate, and liquid inlet pipes and liquid discharge pipes are respectively connected to both side walls of the box. Both liquid inlet pipes and liquid discharge pipes are connected with each other. Valve installed.

Preferably, a sliding seat is hingedly mounted on the top of the screen frame, and the sliding seat is slidably connected to a chute provided on the lower surface of the mounting plate, and both ends of the chute are closed.

Preferably, the screen frame is composed of two detachably enclosed half frames, one end of the two half frames is rotationally connected, and the other end is connected through a snap.

Preferably, the moving mechanism includes:

Horizontally rotate the screw rod installed on the top of the box;

The first step motor fixed on the box is used to drive the screw rod to rotate;

The mounting seat is threadedly connected to the screw rod, the mounting seat is drivingly connected to the partition plate, and the mounting seat is slidingly connected to the top of the box.

Preferably, the mounting base and the partition plate are connected through a driving mechanism, and the driving mechanism includes:

a second stepper motor fixed on the mounting base;

a turntable fixed on the output shaft of the second stepper motor;

The driving pin is eccentrically mounted on the turntable, and the partition plate is provided with a vertical movable slot for the driving pin to be inserted and move.

Preferably, a threaded rod is rotatably mounted on the turntable, and the threaded rod is arranged along the radial direction of the turntable. An internal thread sleeve is threadedly connected to the threaded rod. The internal thread sleeve is slidably connected to the turntable, and the driving pin is fixed. On the internal thread.

Preferably, a plurality of spray parts are installed on both side walls of the partition plate at intervals from top to bottom. The spray parts include a mounting rod and a spray head. One end of the installation rod is hinged to the side wall of the partition plate, so The other end of the mounting rod is equipped with a nozzle for spraying liquid to the screen frame. The box is provided with a liquid supply component for supplying liquid to the nozzle. The box is provided with a power for driving the mounting rod to swing up and down. Mechanism, the power mechanism includes:

Rotate the connecting rod mounted on the drive pin;

The driving rods are fixed at both ends of the connecting rod. The two driving rods are respectively connected with the mounting rods on both sides of the partition plate. The driving rods are arranged vertically and are slidingly connected to the side walls of the partition plate. A plurality of pins corresponding to the mounting rods are fixed, and the mounting rods are provided with through slots for the pins to be inserted and move.

Preferably, the liquid supply component includes:

A self-priming pump fixedly installed on the partition and located below the mounting plate;

Two suction pipes are connected to the inlet of the self-priming pump. The two suction pipes extend to the lower part of the dip dyeing chamber and the cleaning chamber respectively. The two suction pipes are respectively equipped with solenoid valves;

Two liquid supply pipes are connected to the outlet of the self-priming pump. The two liquid supply pipes are connected to the nozzles in the dipping chamber and the cleaning chamber respectively. The two liquid supply pipes are respectively equipped with solenoid valves;

Two trigger switches are symmetrically installed on the partition plate and located on the lower side of the installation plate. The bottom ends of the two trigger switches are rotationally connected to the partition plate and are connected with a reset torsion spring between the two trigger switches. The top of the trigger switch extends to both sides to the lower side of the partition plate. The two trigger switches are electrically connected to the self-priming pump respectively and can control the start and stop of the self-priming pump. Each trigger switch is connected to the suction pipe and the suction pipe on the same side. The solenoid valve on the liquid supply pipe is electrically connected to control the opening and closing of the solenoid valve.

The present invention also provides a processing method based on the above-mentioned oral cancer in vitro tumor tissue specimen processing device, including the following steps:

Obtain oral cancer isolated tumor tissue specimens and place them in the mesh frame;

Add dip dyeing solution into the dip dyeing chamber and add cleaning solution into the cleaning chamber;

The moving mechanism drives the partition plate to move toward the side of the dyeing chamber. At the same time, the sector gear and the rack move relative to each other. The sector gear rotates counterclockwise. The sector gear drives the mounting plate to rotate counterclockwise through the rotating shaft. The mounting plate is located at one end of the dyeing chamber and the mesh. The frame moves downward, enters the dip dyeing solution, and dips for 1-2 hours;

After the dip dyeing treatment, the moving mechanism drives the partition plate to move to the side of the cleaning chamber. At the same time, the sector gear rotates clockwise. The sector gear drives the mounting plate to rotate clockwise through the rotating shaft. The end of the mounting plate located in the cleaning chamber descends into the cleaning fluid. During the rotation of the installation plate, the screen frame slides along the installation plate from the dyeing chamber to the cleaning chamber, enters the cleaning liquid, and is cleaned for 2-4 minutes;

After cleaning, the tumor tissue specimens are sectioned and bleached to obtain specimen sections;

Images of specimen slices are collected through the image scanning acquisition unit, and pathological slice examination and immunohistochemical examination are performed.

Compared with the prior art, the embodiments of the present application mainly have the following beneficial effects:

The oral cancer isolated tumor tissue specimen processing device provided by the present invention is provided with a box, a partition plate, a staining chamber, a cleaning chamber, a mounting plate, a sector gear, a rack, a screen frame and a moving mechanism, and the moving mechanism drives the separation The plate moves to one side of the dyeing chamber, and at the same time the sector gear and the rack move relative to each other. The sector gear rotates counterclockwise. The sector gear drives the installation plate to rotate counterclockwise through the rotating shaft. The end of the installation plate located in the dyeing chamber and the screen frame move downward, entering In the dip dyeing solution; after the dip dyeing treatment, the moving mechanism drives the partition plate to move to the side of the cleaning chamber, and at the same time the sector gear rotates clockwise. The sector gear drives the mounting plate to rotate clockwise through the rotating shaft, and one end of the mounting plate located in the cleaning chamber descends and enters. In the cleaning fluid, during the rotation of the mounting plate, the screen frame slides along the mounting plate from the dyeing chamber to the cleaning chamber, and enters the cleaning fluid, automatically and quickly completing the transfer of tumor tissue specimens from dyeing to cleaning without the need for manual transfer. , improves work efficiency, separates the dip-dyeing chamber and the cleaning chamber, and reduces the interaction between the dip-dying treatment and the cleaning treatment.

Description of drawings

Figure 1 is a schematic structural diagram of an oral cancer isolated tumor tissue specimen processing device provided by the present invention;

Figure 2 is a side view of Figure 1;

Figure 3 is a diagram of the cooperation between the sector gear and the rack in an oral cancer in vitro tumor tissue specimen processing device provided by the present invention;

Figure 4 is an enlarged view of point A in Figure 1;

Figure 5 is an enlarged view of B in Figure 1;

Figure 6 is a flow chart of a method for processing oral cancer in vitro tumor tissue specimens provided in Embodiment 2 of the present invention.

Notes on reference numbers: 1. Box; 2. Cleaning chamber; 3. Mounting plate; 4. Screw; 5. Guide rod; 6. Mounting seat; 7. Partition plate; 8. Turntable; 9. Connecting rod; 10. Threaded rod; 11. Driving rod; 12. Trigger switch; 13. Self-priming pump; 14. Nozzle; 15. Installation rod; 16. Liquid supply pipe; 17. Suction pipe; 18. Sealing strip; 19. Rotating shaft ; 20. Slide seat; 21. Screen frame; 22. First step motor; 23. Dye chamber; 24. Sector gear; 25. Rack; 26. Internal thread sleeve; 27. Driving pin; 28. Second step Enter the motor; 29. Vertical movable slot.

Detailed ways

Unless otherwise defined, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the technical field of this application; the terms used herein in the specification of the application are for the purpose of describing specific embodiments only. , are not intended to limit the application; the terms "including" and "having" and any variations thereof in the description and claims of the application and the above description of the drawings are intended to cover non-exclusive inclusion. The terms "first", "second", etc. in the description and claims of this application or the above-mentioned drawings are used to distinguish different objects, rather than to describe a specific sequence.

Reference herein to "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearances of this phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those skilled in the art understand, both explicitly and implicitly, that the embodiments described herein may be combined with other embodiments.

Example 1

Embodiments of the present invention provide an oral cancer in vitro tumor tissue specimen processing device, as shown in Figures 1 to 5, including:

Box 1. The box 1 is equipped with a door, which can be installed through hinges and buckles. Self-locking rollers can be installed evenly and symmetrically on the bottom of the box 1 to facilitate movement;

The partition plate 7 provided in the box body 1 is used to divide the inner cavity of the box body 1 into a dyeing chamber 23 and a cleaning chamber 2. A plurality of horizontal guide rods 5 are passed through the edge of the partition plate 7. The guide rod 5 is fixedly connected to the side wall of the box 1 and can be fixed by welding. It should be explained that the dip dye chamber 23 is used to contain the dip dye solution, which can be Lugol's iodine solution, and the cleaning chamber 2 is used to hold the dip dye solution. Contains cleaning fluid, which can be PBS buffer. Of course, other existing technologies can also be used, which will not be described in detail. Preferably, sealing strips 18 are installed on the bottom of the partition plate 7 and the front and rear side walls, so The two side walls of the box 1 are respectively connected with liquid inlet pipes and liquid discharge pipes, and valves are installed on the liquid inlet pipes and liquid discharge pipes to facilitate the addition and discharge of liquid dye and cleaning liquid;

The installation plate 3 runs through the partition plate 7. The middle part of the installation plate 3 is rotatably connected to the partition plate 7 through a rotating shaft 19. Both ends of the installation plate 3 are respectively located in the dyeing chamber 23 and the cleaning chamber 2. One end of the rotating shaft 19 Extending to the outside of the box 1, a sector gear 24 is fixed. A rack 25 is fixed on the outer wall of the box 1, which can be fixed by welding. The rack 25 is located on the lower side of the sector gear 24 and meshes with it. The box A long groove is provided on the outer wall of the body 1 for the movement of the rotating shaft 19. A flexible or elastic layer can be set outside the long groove. Both ends of the flexible or elastic layer are adhered to the box body. The rotating shaft 19 penetrates the flexible or elastic layer, and the rotating shaft 19 moves The flexible or elastic layer will not interfere with its movement, and can seal the long groove and reduce the connection with the outside world. Of course, other methods can also be used, such as sliding and installing a sealing plate on the outside of the long groove, with the rotating shaft 19 passing through it. The sealing plate, when the rotating shaft 19 moves, the sealing plate also moves, the length of the sealing plate is longer, and the long slot is always sealed;

The screen frame 21 is slidably installed on the lower surface of the mounting plate 3 and is used to place tumor tissue specimens. The partition plate 7 is provided with a through hole for the screen frame 21 to pass through, and the sliding seat 20 can be hingedly installed on the top of the screen frame 21. The sliding seat 20 is slidingly connected to the chute provided on the lower surface of the mounting plate 3. Both ends of the chute are closed. The screen frame 21 is composed of two detachable half frames, and one end of the two half frames can be rotated and connected. , the other end is connected through a buckle, the screen frame 21 can be spherical, and of course can also be other shapes without too many restrictions;

A moving mechanism installed on the box 1 to drive the partition plate 7 to move horizontally;

Initially, as shown in Figure 1, the partition plate 7 is located in the middle of the inner cavity of the box 1. Open the door, put the tumor tissue specimen into the screen frame 21, and drive the partition plate 7 toward the staining chamber 23 through the moving mechanism. side movement, the liquid level in the dyeing chamber 23 rises. At the same time, the sector gear 24 and the rack 25 move relative to each other, causing the sector gear 24 to rotate counterclockwise. The sector gear 24 drives the mounting plate 3 to rotate counterclockwise through the rotating shaft 19. The mounting plate 3 is located at One end of the dip dyeing chamber 23 and the screen frame 21 move downward and enter the dip dyeing solution. After the dip dyeing process is completed, the moving mechanism drives the partition plate 7 to move to the side of the cleaning chamber 2, and the liquid level in the cleaning chamber 2 rises. , the sector gear 24 rotates clockwise. The sector gear 24 drives the mounting plate 3 to rotate clockwise through the rotating shaft 19. The end of the mounting plate 3 located in the cleaning chamber 2 descends into the cleaning liquid. During the rotation of the mounting plate 3, the screen frame 21 moves along the The mounting plate 3 slides from the dip dyeing chamber 23 to the cleaning chamber 2, enters the cleaning fluid, and is cleaned by the cleaning fluid, automatically and quickly completing the transfer of the tumor tissue specimen from the dip dyeing process to the cleaning process, without the need for manual transfer, which improves work efficiency. , separate the dip-dyeing chamber 23 and the cleaning chamber 2 to reduce the mutual influence between the dip-dying treatment and the cleaning treatment.

Wherein, the moving mechanism includes:

The screw rod 4 is horizontally rotated and installed on the top of the box 1 and can be installed through bearing rotation;

The first step motor 22 fixed on the box 1 is used to drive the screw rod 4 to rotate and can be fixed by bolts. The first step motor 22 is a forward and reverse motor, and the output shaft of the first step motor 22 can be Fixed connection with one end of screw rod 4 through coupling;

The mounting seat 6 is threadedly connected to the screw rod 4. The mounting seat 6 is drivingly connected to the partition plate 7. The mounting seat 6 can be slidably connected to the top of the box 1;

It can be understood that when the first stepping motor 22 is started, the first stepping motor 22 drives the screw rod 4 to rotate, the screw rod 4 drives the mounting base 6 to move, and the mounting base 6 drives the partition plate 7 to move;

Specifically, the mounting base 6 and the partition plate 7 are connected through a driving mechanism, and the driving mechanism includes:

The second stepper motor 28 fixed on the mounting base 6 can be fixed by bolts;

The turntable 8 fixed on the output shaft of the second stepper motor 28 can be fixed by welding;

The driving pin 27 is eccentrically mounted on the turntable 8, and the partition plate 7 is provided with a vertical movable slot 29 for the driving pin 27 to be inserted and move;

Start the second stepper motor 28. The second stepper motor 28 drives the turntable 8 to rotate. The turntable 8 drives the driving pin 27 to rotate. The driving pin 27 drives the partition plate 7 to reciprocate left and right. The partition plate 7 drives the dyeing chamber 23 and the cleaning chamber. The liquid in 2 continuously flows, thereby continuously impacting the tumor tissue specimens in the screen frame 21, which helps to improve the efficiency of dyeing and cleaning. At the same time, the partition plate 7 drives the mounting plate 3 and the screen frame 21 on it to reciprocate left and right. , and, with the cooperation of the sector gear 24 and the rack 25, the mounting plate 3 swings back and forth, driving the screen frame 21 to swing back and forth, and the tumor tissue specimens in the screen frame 21 continuously move in the liquid, further improving the staining and cleaning efficiency. The amount of liquid added into the dyeing chamber 23 and the cleaning chamber 2 and the left and right reciprocating distance of the partition plate 7 can meet the requirements that the liquid will not flow to the other side through the through hole, without excessive restrictions.

In this embodiment, a threaded rod 10 is rotatably mounted on the turntable 8. The threaded rod 10 is arranged radially along the turntable 8. An internally threaded sleeve 26 is threadedly connected to the threaded rod 10. The internally threaded sleeve 26 and The turntable 8 is slidingly connected, and the driving pin 27 is fixed on the internal thread sleeve 26, which can be fixed by welding. By rotating the threaded rod 10, the threaded rod 10 drives the internal thread sleeve 26 to move radially along the turntable 8, and the internal thread sleeve 26 drives The driving pin 27 moves to adjust its position on the turntable 8 and its range of movement, thereby adjusting the reciprocating range of the partition plate 7 and adjusting the liquid flow effect;

In a specific implementation, a plurality of spray parts are installed on both sides of the partition plate 7 at intervals from top to bottom. The spray parts include a mounting rod 15 and a nozzle 14. One end of the mounting rod 15 is connected to the partition. The side wall of the board 7 is hinged, and the other end of the mounting rod 15 is equipped with a nozzle 14 for spraying liquid to the screen frame 21. The nozzle 14 can be installed through screws. The box 1 is provided with a liquid supply component for supplying the nozzle. 14 supplies liquid. Preferably, the box 1 is provided with a power mechanism for driving the mounting rod 15 to swing up and down. The power mechanism includes:

Rotate the connecting rod 9 installed on the driving pin 27;

The driving rods 11 fixed at both ends of the connecting rod 9 can be fixed by welding. The two driving rods 11 are respectively connected with the mounting rods 15 on both sides of the partition plate 7. The driving rods 11 are arranged vertically and connected with the partition. The side walls of the plate 7 are slidingly connected through a sliding block and a chute. The driving rod 11 is fixed with a plurality of pins corresponding to the mounting rod 15, and the mounting rod 15 is provided with a supply pin. The slot into which the shaft is inserted and moved;

It can be understood that when the turntable 8 drives the driving pin 27 to rotate, the driving pin 27 drives the driving rod 11 to move up and down through the connecting rod 9, the driving rod 11 drives the mounting rod 15 to swing up and down through the pin, and the mounting rod 15 drives the nozzle 14 to swing up and down. It can eject liquid at multiple angles and in a wide range, so that the liquid fully contacts and impacts the tumor tissue specimen in the screen frame 21, thereby improving the effect of staining and cleaning;

In this embodiment, the liquid supply component includes:

The self-priming pump 13 fixedly installed on the partition and located below the installation plate 3 can be installed through bolts;

Two suction pipes 17 are connected to the inlet of the self-priming pump 13. The two suction pipes 17 extend to the lower part of the dyeing chamber 23 and the cleaning chamber 2 respectively. The two suction pipes 17 are respectively equipped with solenoid valves;

Two liquid supply pipes 16 are connected to the outlet of the self-priming pump 13. The two liquid supply pipes 16 are respectively connected with the nozzles 14 in the dyeing chamber 23 and the cleaning chamber 2. Solenoid valves are respectively installed on the two liquid supply pipes 16;

Two trigger switches 12 are symmetrically installed on the partition plate 7 and located on the lower side of the installation plate 3. In this embodiment, the trigger switches 12 are L-shaped, and the bottom ends of the two trigger switches 12 are in contact with the partition. The plate 7 is rotationally connected and is connected with a return torsion spring with the partition plate 7 . The top ends of the two trigger switches 12 extend to both sides to the lower side of the partition plate 7 . Preferably, the top ends of the trigger switches 12 are under the partition plate 7 The distance between the surfaces is 1cm. The two trigger switches 12 are electrically connected to the self-priming pump 13 respectively and can control the start and stop of the self-priming pump 13. Each trigger switch 12 is connected to the suction pipe 17 and the liquid supply pipe 16 on the same side. The solenoid valve is electrically connected to control the opening and closing of the solenoid valve;

What needs to be explained is that when the left end of the installation plate 3 rotates downward and enters the dyeing chamber 23, the installation plate 3 presses the left trigger switch 12 to push it to rotate. The left trigger switch 12 controls the self-priming pump 13 to start, and at the same time the dyeing chamber The solenoid valves on the suction pipe 17 and liquid supply pipe 16 in 23 are opened, the self-priming pump 13 sucks the liquid from the liquid suction pipe 17 in the dyeing chamber 23, and then pumps the liquid into the liquid supply pipe in the dyeing chamber 23 16, causing the nozzle 14 in the dip dyeing chamber 23 to spray out the dip dye. After the dip dyeing process is completed, the mounting plate 3 rotates and resets, and is separated from the left trigger switch 12. The self-priming pump 13 stops, and the suction pipe 17, The solenoid valve on the liquid supply pipe 16 is closed, and then the right end of the installation plate 3 rotates downward into the cleaning chamber 2. The installation plate 3 presses the right trigger switch 12 to push it to rotate. The right trigger switch 12 controls the self-priming pump 13 to start. The solenoid valves on the suction pipe 17 and the liquid supply pipe 16 in the cleaning chamber 2 are opened, the self-priming pump 13 sucks the cleaning liquid from the suction pipe 17 in the cleaning cavity 2, and then pumps the cleaning liquid into the cleaning cavity 2. The liquid supply pipe 16 causes the nozzle 14 in the cleaning chamber 2 to eject cleaning liquid. After the cleaning is completed, the moving mechanism drives the partition plate 7 to reset, and the mounting plate 3 resets to a horizontal state.

To sum up, embodiments of the present invention provide an oral cancer in vitro tumor tissue specimen processing device, the working principle of which is:

Open the door, put the tumor tissue specimen into the mesh frame 21, and drive the partition plate 7 to move to the side of the dyeing chamber 23 through the moving mechanism. The liquid level in the dyeing chamber 23 rises. At the same time, the sector gear 24 faces the rack 25. Movement causes the sector gear 24 to rotate counterclockwise. The sector gear 24 drives the installation plate 3 to rotate counterclockwise through the rotating shaft 19. The end of the installation plate 3 located in the dip chamber 23 and the screen frame 21 move downward and enter the dip solution. At the same time, the installation plate 3 Press down the left trigger switch 12 to push it to rotate. The left trigger switch 12 controls the start of the self-priming pump 13. At the same time, the solenoid valves on the suction pipe 17 and the liquid supply pipe 16 in the dyeing chamber 23 are opened, and the self-priming pump 13 The liquid is sucked in from the suction pipe 17 in the dyeing chamber 23, and then the liquid is pumped into the liquid supply pipe 16 in the dyeing chamber 23, so that the nozzle 14 in the dyeing chamber 23 sprays the liquid. After the dyeing process is completed, the liquid is moved by moving The mechanism drives the partition plate 7 to move toward the side of the cleaning chamber 2. The liquid level in the cleaning chamber 2 rises. At the same time, the sector gear 24 rotates clockwise. The sector gear 24 drives the mounting plate 3 to rotate clockwise through the rotating shaft 19. The mounting plate 3 is located at One end of the cleaning chamber 2 descends into the cleaning fluid. During the rotation of the mounting plate 3, the screen frame 21 slides along the mounting plate 3 from the dyeing chamber 23 to the cleaning chamber 2 and enters the cleaning fluid. At the same time, the mounting plate 3 is pressed down. The right trigger switch 12 pushes it to rotate. The right trigger switch 12 controls the start of the self-priming pump 13. At the same time, the solenoid valves on the suction pipe 17 and the liquid supply pipe 16 in the cleaning chamber 2 are opened. The self-priming pump 13 starts from the cleaning chamber 2. The suction pipe 17 in 2 sucks the cleaning liquid, and then pumps the cleaning liquid into the liquid supply pipe 16 in the cleaning chamber 2, so that the nozzle 14 in the cleaning cavity 2 sprays the cleaning liquid. After the cleaning is completed, the moving mechanism drives the partition plate 7 is reset, the mounting plate 3 is reset to a horizontal state, the door is opened, and the tumor tissue specimens in the screen frame 21 are taken out for testing.

Example 2

The embodiment of the present invention provides a processing method based on the oral cancer in vitro tumor tissue specimen processing device in Embodiment 1, as shown in Figure 6, including the following steps:

Obtain oral cancer isolated tumor tissue specimens (primary tumor specimens and lymph node specimens) and place them in the mesh frame 21;

Add the dyeing liquid into the dyeing chamber 23, and add the cleaning liquid into the cleaning chamber 2;

The moving mechanism drives the partition plate 7 to move toward the dye chamber 23 side. At the same time, the sector gear 24 and the rack 25 move relative to each other. The sector gear 24 rotates counterclockwise. The sector gear 24 drives the mounting plate 3 to rotate counterclockwise through the rotating shaft 19. The mounting plate 3. The end located in the dyeing chamber 23 and the screen frame 21 move downward, enter the dyeing liquid, and dip for 1-2 hours;

After the dip dyeing treatment, the partition plate 7 is driven by the moving mechanism to move to the side of the cleaning chamber 2. At the same time, the sector gear 24 rotates clockwise. The sector gear 24 drives the mounting plate 3 to rotate clockwise through the rotating shaft 19. The mounting plate 3 is located in the cleaning chamber 2. One end of the screen is lowered into the cleaning fluid. During the rotation of the mounting plate 3, the screen frame 21 slides along the mounting plate 3 from the dyeing chamber 23 to the cleaning chamber 2, enters the cleaning fluid, and is cleaned for 2-4 minutes;

After cleaning, the tumor tissue specimens are sectioned and bleached to obtain specimen sections;

Images of specimen slices are collected through the image scanning and acquisition unit, and pathological biopsy and immunohistochemical examination are performed. Specifically, the image scanning and acquisition unit may use equipment in the existing technology.

It should be noted that for the sake of simple description, the foregoing embodiments are all expressed as a series of action combinations. However, those skilled in the art should know that the present invention is not limited by the described action sequence, because Certain steps may be performed in other orders or simultaneously in accordance with the invention. Secondly, those skilled in the art should also know that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily necessary for the present invention.

The above embodiments are only used to illustrate the technical solutions of the present invention, but are not intended to limit the scope of protection of the invention. Obviously, the described embodiments are only some, but not all, of the embodiments of the present invention. Based on these embodiments, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

Claims (10)

1. An oral cancer in vitro tumor tissue specimen processing device, which is characterized in that it includes: box; A partition plate provided in the box is used to divide the inner cavity of the box into a dyeing chamber and a cleaning chamber. A plurality of horizontal guide rods are passed through the edge of the partition plate, and the guide rods are fixedly connected to the side walls of the box. ; A mounting plate that runs through the partition plate. The middle part of the mounting plate is rotationally connected to the partition plate through a rotating shaft. Both ends of the mounting plate are located in the dyeing chamber and the cleaning chamber respectively. One end of the rotating shaft extends to the outside of the box and is fixed with a fan. Gear, a rack is fixed on the outer wall of the box, the rack is located on the lower side of the sector gear and meshes with it, and a long slot for the movement of the rotating shaft is provided on the outer wall of the box; A mesh frame is slidably installed on the lower surface of the installation plate and is used to place tumor tissue specimens. The partition plate is provided with a through hole for the mesh frame to pass; A moving mechanism installed on the box to drive the partition board to move horizontally. 2. The oral cancer in vitro tumor tissue specimen processing device according to claim 1, characterized in that sealing strips are installed on the bottom of the partition board and the front and rear side walls, and the two side walls of the box are respectively connected. There is a liquid inlet pipe and a liquid discharge pipe, and valves are installed on the liquid inlet pipe and the liquid discharge pipe. 3. The device for processing isolated oral cancer tumor tissue specimens according to claim 1, characterized in that a sliding seat is hingedly installed on the top of the screen frame, and the sliding seat is slidingly connected to a chute provided on the lower surface of the mounting plate, so Both ends of the chute are closed. 4. The oral cancer isolated tumor tissue specimen processing device according to claim 1, characterized in that the mesh frame is composed of two detachable half frames, one end of the two half frames is rotatably connected, and the other end is connected by a buckle. connect. 5. The oral cancer in vitro tumor tissue specimen processing device according to claim 1, wherein the moving mechanism includes: Horizontally rotate the screw rod installed on the top of the box; The first step motor fixed on the box is used to drive the screw rod to rotate; The mounting seat is threadedly connected to the screw rod, the mounting seat is drivingly connected to the partition plate, and the mounting seat is slidingly connected to the top of the box. 6. The oral cancer in vitro tumor tissue specimen processing device according to claim 5, wherein the mounting base and the partition plate are connected through a driving mechanism, and the driving mechanism includes: a second stepper motor fixed on the mounting base; a turntable fixed on the output shaft of the second stepper motor; The driving pin is eccentrically mounted on the turntable, and the partition plate is provided with a vertical movable slot for the driving pin to be inserted and move. 7. The device for processing isolated oral cancer tumor tissue specimens according to claim 6, wherein a threaded rod is rotatably mounted on the turntable, the threaded rod is arranged radially along the turntable, and the threaded rod is threadedly connected. There is an internal thread sleeve, the internal thread sleeve is slidingly connected to the turntable, and the driving pin is fixed on the internal thread sleeve. 8. The device for processing isolated oral cancer tumor tissue specimens according to claim 6, wherein a plurality of ejection parts are installed at intervals from top to bottom on both side walls of the partition plate, and the ejection parts are It includes a mounting rod and a nozzle. One end of the mounting rod is hinged to the side wall of the partition plate. The other end of the mounting rod is equipped with a nozzle for spraying liquid to the screen frame. The box is provided with a liquid supply component for To supply liquid to the nozzle, the box is provided with a power mechanism for driving the mounting rod to swing up and down. The power mechanism includes: Rotate the connecting rod mounted on the drive pin; The driving rods are fixed at both ends of the connecting rod. The two driving rods are respectively connected with the mounting rods on both sides of the partition plate. The driving rods are arranged vertically and are slidingly connected to the side walls of the partition plate. A plurality of pins corresponding to the mounting rods are fixed, and the mounting rods are provided with through slots for the pins to be inserted and move. 9. The oral cancer in vitro tumor tissue specimen processing device according to claim 8, wherein the liquid supply component includes: A self-priming pump fixedly installed on the partition plate and located below the mounting plate; Two suction pipes are connected to the inlet of the self-priming pump. The two suction pipes extend to the lower part of the dip dyeing chamber and the cleaning chamber respectively. The two suction pipes are respectively equipped with solenoid valves; Two liquid supply pipes are connected to the outlet of the self-priming pump. The two liquid supply pipes are connected to the nozzles in the dipping chamber and the cleaning chamber respectively. The two liquid supply pipes are respectively equipped with solenoid valves; Two trigger switches are symmetrically installed on the partition plate and located on the lower side of the installation plate. The bottom ends of the two trigger switches are rotationally connected to the partition plate and are connected with a reset torsion spring between the two trigger switches. The top of the trigger switch extends to both sides to the lower side of the partition plate. The two trigger switches are electrically connected to the self-priming pump respectively and can control the start and stop of the self-priming pump. Each trigger switch is connected to the suction pipe and the suction pipe on the same side. The solenoid valve on the liquid supply pipe is electrically connected to control the opening and closing of the solenoid valve. 10. The processing method based on the oral cancer in vitro tumor tissue specimen processing device according to any one of claims 1 to 9, characterized in that it includes the following steps: Obtain oral cancer isolated tumor tissue specimens and place them in the mesh frame; Add dip dyeing solution into the dip dyeing chamber and add cleaning solution into the cleaning chamber; The moving mechanism drives the partition plate to move toward the side of the dyeing chamber. At the same time, the sector gear and the rack move relative to each other. The sector gear rotates counterclockwise. The sector gear drives the mounting plate to rotate counterclockwise through the rotating shaft. The mounting plate is located at one end of the dyeing chamber and the mesh. The frame moves downward, enters the dip dyeing solution, and dips for 1-2 hours; After the dip dyeing treatment, the moving mechanism drives the partition plate to move to the side of the cleaning chamber. At the same time, the sector gear rotates clockwise. The sector gear drives the mounting plate to rotate clockwise through the rotating shaft. The end of the mounting plate located in the cleaning chamber descends into the cleaning fluid. During the rotation of the installation plate, the screen frame slides along the installation plate from the dyeing chamber to the cleaning chamber, enters the cleaning liquid, and is cleaned for 2-4 minutes; After cleaning, the tumor tissue specimens are sectioned and bleached to obtain specimen sections; Images of specimen slices are collected through the image scanning acquisition unit, and pathological biopsy and immunohistochemical examination are performed.