CN117305104B - Scientific experiment system with multiple physical fields - Google Patents
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Abstract
The invention provides a multi-physical-field scientific experiment system, which relates to the field of biomedical engineering and comprises the following components: a multi-physical field generating means for generating at least one form of physical field; the multi-physical field control system is used for controlling the working mode of the multi-physical field and monitoring physical field parameters and experimental processes in real time; and the biological experiment device is configured to bear a biological sample, and the biological experiment device receives the physical fields generated by the multi-physical-field generating device so as to regulate and control the functions of the biological sample. The multi-physical-field scientific experiment system provided by the invention is simple to operate and convenient to use, can accurately act different forms of physical field energy on specific biological tissues, cells and molecules in the biotechnology and medical research process, regulate and control functions of the specific biological tissues, cells and molecules, can realize that different physical fields are applied to different positions in one biological experiment device, and is beneficial to comparative experiment analysis in the experiment process.
Description
Technical Field
The invention relates to the field of biomedical engineering, in particular to a multi-physical-field scientific experiment system.
Background
Currently, numerous studies have found that the physical energy field can regulate the function of living beings at different levels. Because of low cost, minimal trauma, small green side effect, no dose limitation, strong controllability, and particularly the well-proven definite effect of a part of physical energy, certain determined energy modes have been gradually applied to the fields of tumor, cardiovascular and cerebrovascular disease treatment, coagulation and cutting in operation, beauty and shaping including subcutaneous fat ablation, tissue rewarming after low-temperature preservation and bacterial virus killing through combination with materials.
On the cell level, research discovers that after the actions of radio frequency, microwave, continuous laser and the like with special high energy, cells can undergo apoptosis or necrosis, and immunogenic intracellular substances such as RNA, DNA, heat shock protein (HSP 70) and the like can be released, and meanwhile, the production of pro-inflammatory cytokines (such as IL-1 and IL-6) and chemokines can be stimulated; unlike these special high-energy and high-temperature effect-generating RF, other specific frequencies can regulate and control the cell functions, such as 500kHz RF energy, collagen fibers can be produced by the dermis fibroblast, and intermittent 448kHz RF is found to increase the cell activity and promote the cell growth recovery; the high-frequency electric field with the transmembrane potential difference of about 500mV can only open the cell membrane and introduce special genes and even proteins, thereby regulating the growth rule of the cells, the functions of the cells and the like. In the freezing treatment, the generation of extracellular ice crystals can lead to the increase of intracellular osmotic pressure, cells gradually lose water and shrink, and in the rewarming stage, the extracellular ice crystals are fused first, so that the cells become a hypertonic state, the cells absorb water and are broken, and broken cell fragments can also be used as antigens to induce immune response; in irreversible electroporation, a pulsed electric field mainly acts on a cell membrane, so that cell rupture occurs at the cell level, and intracellular antigens (including proteins and the like) are released intact and not inactivated.
On the tissue level, after the actions of radio frequency, microwave, continuous laser and the like, the radio frequency induces the reciprocating motion friction of charged particles such as Na+, K+, cl-, ca2+ and the like in the tissue to generate heat, microwave energy drives inherent dipole polar molecules (such as water molecules) in the tissue, the electromagnetic field continuously changes to improve the local tissue temperature, the laser interacts with water or hemoglobin in biological tissue to generate heat, according to different waveforms, different temperatures and different action time, tissue cutting (more than 100 ℃) and thermal coagulation can be realized, congestion is generated in the edge area of the thermal coagulation tissue, the local oxygen content is increased, active oxygen is stimulated to generate, indirect killing is caused, and meanwhile, antigens released by thermal coagulation can induce immune cell infiltration tissues such as macrophages, dendritic cells and T cells to activate immune response; at the same time, high temperature (higher than 60 ℃) can also carry out plasticity on collagen fibers in the tissues, change the forms of the tissues, lift the skin, carry out plasticity on vascular tissues, prop open narrow blood vessels or close varicose veins (different depending on the acting temperature), and the like; the low temperature can directly damage the vascular structure of the tissue, can increase vascular permeability, can cause platelet aggregation to form micro thrombus, and can induce infiltration of immune cells due to antigen released by cells after freezing to activate the immune response of the organism. The low temperature can also reduce metabolism of biological tissues, maintain the structure and function of the cell tissues and keep the functions of the tissue organs for a long time.
In summary, current biomedical research has found that specific patterns of physical energy fields (power, frequency, electric field strength, pulse strength, waveform and phase, and freezing temperature) can produce distinct effects on cells and biological tissues, and can be used to effect modulation and modification of certain biological tissue functions.
Therefore, in view of the capability of energy of different physical fields in terms of cell, tissue and biological experiments at different levels, the invention overcomes the bottleneck of the prior art, and provides a design of a multi-physical scientific experiment system, which combines a plurality of physical fields on one system, and can independently and randomly regulate and control the energy generated by different physical fields and transmit the energy to a scientific experiment device. The method provides a research means for innovative scientific basic researches of more biologists and medical staff, not only promotes a series of novel original methods and novel technologies facing human health and biotechnology, but also greatly improves the existing technologies in the process of researching the project.
Disclosure of Invention
The invention mainly aims to provide a multi-physical-field scientific experiment system which can accurately act different forms of physical field energy on specific biological tissues, cells and molecules to regulate and control functions of the specific biological tissues, cells and molecules, and can realize that different physical fields are applied to different positions in one biological experiment device, thereby being beneficial to comparative experiment analysis in the experiment process.
In a first aspect, an embodiment of the present invention provides a multi-physical field scientific experiment system, including: a multi-physical field generating means for generating at least one form of physical field; the multi-physical field control system is used for controlling the working mode of the multi-physical field and monitoring physical field parameters and experimental processes in real time; and a biological experiment device configured to carry a biological sample, and receive the physical fields transmitted by the multi-physical field generating device through the biological experiment device so as to regulate the functions of the biological sample.
In some embodiments, the system further comprises a control module for controlling the multi-physical field generating device, the multi-physical field transmission module and the biological experiment device.
In some embodiments, the control module comprises an industrial control computer.
In some embodiments, the system further comprises an image acquisition module, wherein the image acquisition module is connected with the control module and is used for recording images of the actual change process of the biological sample in the biological experimental device in real time.
In some embodiments, the image acquisition module comprises a CCD image acquisition device mounted on a confocal microscope.
In some embodiments, the system further comprises an order entry module for a user to enter working parameters of the multi-physical field scientific experiment system.
In some embodiments, the instruction entry module includes a touch display screen.
In some embodiments, the biological assay device comprises a probe, a cavity, and a platform for a field of view assay sample.
In some embodiments, the biological assay device further comprises a cell culture dish.
In some embodiments, the cell culture dish is configured with a refrigeration module.
In some embodiments, the cell culture dish is configured with a temperature monitoring module.
In some embodiments, the cell culture dish is configured with a defrost channel.
The multi-physical-field scientific experiment system provided by the embodiment is simple to operate and convenient to use, can accurately act on specific biological tissues, cells and molecules by using different forms of physical field energy in the biotechnology and medical research process, regulates and controls functions of the specific biological tissues, cells and molecules, can apply different physical fields at different positions in one scientific experiment device, and is beneficial to comparative experiment analysis in the experiment process.
In addition, because the image acquisition module is adopted to record the experimental process in real time, the observability and the subsequent data analysis in the scientific experimental process can be improved.
The multi-physical-field scientific experiment system provided by the embodiment is beneficial to promoting new methods and new technical researches of human health and biotechnology, and provides research means for innovative scientific basic researches of biologists and medical staff.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a multi-physical field scientific experiment system according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a multi-physical field generating device according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a cell culture apparatus according to one embodiment of the present invention;
Fig. 4 is a schematic diagram of a liquid nitrogen phase transformation control module according to an embodiment of the present invention.
Icon: the device comprises a 1-biological experiment device, a 2-multi-physical-field generating device, a 3-multi-physical-field control system, a 31-control module, a 32-input display module, a 33-storage module, a 34-data analysis unit, a 35-image acquisition module, a 36-data acquisition module, a 11-cell culture dish, a 12-multi-physical-field electrode array, a 13-temperature sensor array, a 14-liquid nitrogen phase-change heat exchange channel, a 15-defrosting channel, a 21-multi-physical-field system power module, a 211-power pretreatment module, a 212-DC module, a 213-high-voltage adjustable power module, a 214-adjustable radio-frequency power module, a 215-spike wave power module, a 216-adjustable constant current source, a 22-microcontroller, a 23-multi-physical-field generating module, a 231-high-voltage pulse generating module, a 232-radio-frequency generating module, a 233-spike wave generating module, a 234-direct-current heating module, a 235-liquid nitrogen control module, a 24-multi-physical-field selection output module, a 25-multi-physical-field transmission module, a 251-physical-field transmission channel, a 252-low-temperature-electric signal transmission channel, a 2355-small liquid nitrogen electric signal, a 2352-vacuum tank, a 2352-negative pressure electromagnetic valve and a 2353-negative pressure electromagnetic valve.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal," "vertical," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
The following describes the structure of a multi-physical-field scientific experimental system provided in this embodiment with reference to fig. 1 to 4.
The multi-physical-field scientific experiment system provided by the embodiment comprises a biological experiment device 1, a multi-physical-field generation device 2 and a multi-physical-field control system 3.
The multi-physical field generating device 2 is used for generating various physical fields in different forms, so that the physical fields are accurately transmitted to the biological experiment device 1, and the different physical fields mainly comprise an electric field of radio frequency, a pulse electric field, a microwave field, a radio frequency magnetic field, a pulse magnetic field, a microwave magnetic field, a high-temperature thermal field, a low-temperature thermal field and the like.
The biological experiment device 1 is used for receiving the physical field energy transmitted by the multi-physical field generating device 2, and transmitting the corresponding physical field energy to a specific position in the biological experiment device 1 through the multi-physical field transmission module 25 to regulate and control the function of a biological sample.
The multi-physical-field control system 3 is used for controlling the working states of the multi-physical-field, inputting control parameters, collecting experimental process information, storing and analyzing data and the like, and mainly comprises a control module 31 (which can be an industrial control computer), an input display module 32 (which can be a touch display screen), a storage module 33, a data analysis unit 34, an image acquisition module 35 and a data acquisition module 36.
The image acquisition module 35 can be installed on a confocal microscope, and the image acquisition module is used for observing and recording the actual change process of the biological sample in the biological experiment device 1 in real time.
The control module 31 is used as a carrier of the multi-physical-field precise control algorithm, controls the working process of the whole system, simultaneously collects and processes the image information transmitted by the image acquisition module 35, drives the input display module 32 to display the image information on a screen, and stores the image information in the storage module 33.
The input display module is used for setting working parameters of the multi-physical-field scientific experiment system and displaying physical field information, temperature information, experimental process image information and the like in the experimental process.
In this embodiment, the multi-physical field generating device 2 mainly includes a multi-physical field system power module 21, a microcontroller 22, a multi-physical field generating module 23, a multi-physical field selecting output module 24, and a multi-physical field transmitting module 25.
The multi-physical-field system power module 21 includes a power preprocessing module 211, a dc module 212, a high-voltage adjustable power module 213, an adjustable radio frequency power module 214, a spike power module 215, and an adjustable constant current source 216.
The multi-physical field generating module 23 comprises a high-voltage pulse generating module 231, a radio frequency generating module 232, a spike wave generating module 233, a direct current heating module 234 and a liquid nitrogen phase change control module 235.
The multiple physical field transmission module 25 includes an electrical signal physical field transmission channel 251 and a low temperature field transmission channel 252.
The microcontroller 22 controls and selects multiple adjustable power supply modules in the multiple physical field system power supply module 21, controls the output power, voltage, current and the like of the multiple physical field generating module 23 corresponding to the physical field by controlling the output of the adjustable power supply, selects the output of at least one physical field by controlling the multiple physical field selecting output module 24, and finally transmits the physical field energy to the biological experiment device 1 for scientific experiment research by the multiple physical field transmitting module 25.
In this embodiment, the high-voltage pulse module 231 outputs control information such as frequency, pulse width, pulse number, pulse period, etc. of the high-voltage pulse by adopting a PWM control manner through an advanced timer of the microcontroller 22, and simultaneously controls the output of the high-voltage adjustable power supply module 213 through the microcontroller 22 to control the output voltage amplitude and power of the high-voltage pulse module. The pulse voltage amplitude is adjustable between 0 KV and 5KV, and the pulse voltage is used for adapting to the output of different forms of physical field energy. Such as a steep pulsed electric field forming a high voltage, an electric field of square wave, a radio frequency ablation field, etc.
In this embodiment, the rf generating module 232 outputs the rf baseband signal with the response frequency by controlling the DDS signal of the microprocessor 22, inputs the rf baseband signal to the rf amplifier for power amplification, outputs the amplified rf baseband signal, controls the adjustable rf power module 214 by the microprocessor 22, controls the output power of the rf power amplifier, and simultaneously controls the modulation frequency of the rf by the microprocessor 22 to change the rf output mode, thereby changing the rf thermal field, the rf electric field, or the rf electromagnetic field mode.
In this embodiment, the liquid nitrogen phase change control module 235 adopts the setting of the control of a plurality of groups of micro liquid nitrogen phase change channels, and comprises a liquid nitrogen liquid inlet channel, a liquid nitrogen discharge channel, a negative pressure regulating and controlling proportional valve, a gas three-way valve and a small liquid nitrogen bottle, wherein the liquid nitrogen phase change control module is connected with the micro phase change heat exchange channel in the scientific experiment device 1 in the working process, the negative pressure regulating and controlling proportional valve regulates and controls the negative pressure quantity of the nitrogen discharge channel, adjusts the flow rate of the liquid nitrogen liquid inlet channel, and controls the gas-liquid ratio of the liquid nitrogen in the micro phase change heat exchange channel in the scientific experiment device 1, thereby controlling the temperature of the phase change heat exchange channel, namely controlling the regulating and controlling temperature of the corresponding position in the scientific experiment device 1.
In this embodiment, the biological test device 1 includes a biological test device in the form of a probe for tissue test, a multi-physical field balloon, a catheter, a test cavity, a cell culture device, and the like.
In this embodiment, the cell culture apparatus in the biological experiment device 1 at least comprises a cell culture dish 11, a multi-physical field electrode array 12, a temperature sensor array 13, a multi-path liquid nitrogen phase change heat exchange channel 14 and a defrosting channel 15.
The multi-channel liquid nitrogen phase change heat exchange channels 14 are uniformly distributed on the cell culture dish 11 and are used for generating low temperature through phase change heat of liquid nitrogen, then heat conducting glass is designed on the heat conducting channel, meanwhile, a multi-physical field electrode array 12 is designed on the glass and is used for transmitting different forms of physical field energy, and meanwhile, a temperature sensor array 13 is designed at a specific position near an electrode and is used for collecting the temperature in the cell culture process in real time; defrosting channels 15 are designed on the side surfaces of the culture dishes, and nitrogen after phase change heat exchange flows into the channels, so that frosting in the freezing process is avoided, and experimental observation vision is affected.
In this embodiment, the multi-physical field electrode array 12 is made of transparent conductive glass ITO material, and is engraved on the glass by using micro-scale processing technology, and each electrode is independent of the other, and is led out by a micro-wire, so as to transmit various physical field energies.
In this embodiment, the liquid nitrogen phase change heat exchange channel 14 adopts a liquid nitrogen phase change heat exchange channel control device to control the liquid nitrogen heat exchange process. It is connected to the low temperature field transmission channel 252, and the phase change heat exchange process is controlled by the liquid nitrogen phase change control module 235. Thereby controlling the culture temperature of the cells at the corresponding positions.
In this embodiment, the nitrogen gas discharge channel of the liquid nitrogen phase-change heat exchange control module 235 is provided with a gas three-way valve, so that the nitrogen gas after heat exchange can be circulated into the cell culture device to defrost by controlling the gas three-way valve, and the frosting inside the culture dish is inhibited in the low-temperature cell culture process, so that the view field is affected in the culture process.
In the embodiment, a high-definition CCD camera is optionally matched for a cell culture scientific experiment in a multi-physical-field scientific experiment system and is arranged on a lens of a confocal microscope, so that the growth condition of cells in the experimental process can be photographed in real time, and the method is more intuitively used for data analysis.
The multi-physical-field scientific experiment system provided by the embodiment is simple to operate and convenient to use, can accurately act on different forms of physical field energy in a specific biological sample in the biological scientific experiment process, regulates and controls functions of the biological sample, can apply different physical fields at different positions in one scientific experiment device, and is beneficial to the comparative analysis of scientific experiments. Meanwhile, the high-definition image acquisition module is adopted to record the experimental process in real time, so that the observability and the subsequent data analysis in the scientific experimental process are improved. The novel method and the novel technical research which are beneficial to promoting human health and biotechnology are provided by the embodiment, and research means are provided for innovative scientific basic research of biologists and medical staff.
The following describes the working procedure of the multi-physical-field scientific experimental system provided in this embodiment with reference to fig. 1 to 4.
As shown in fig. 1, an input display module 32 of a multi-physical field control system 3 sets experimental parameters and selects experimental modes according to experimental requirements, the experimental parameters and modes are transmitted to a control module 31, the control module 31 calculates multi-physical field control parameters and control methods by adopting a multi-physical field accurate control algorithm according to the set experimental parameters and modes, a command for controlling corresponding physical field operation by a multi-physical field generating device 2 is issued, so that the multi-physical field generating module 2 accurately acts physical field energy on a specific position in a biological experimental device 1, relevant sensors in the biological experimental device 1 collect data in the experimental process in real time, and the data are transmitted to the control module 31 for output processing analysis after being preprocessed in the multi-physical field generating device 2 and stored in a storage module 33.
Meanwhile, the biological experiment device 1 can be optionally placed in a confocal microscope, an image acquisition module 35 is configured on the microscope, or the image acquisition module 35 is directly configured in an experiment field of view, image information of a biological experiment process in the biological experiment device 1 is acquired in real time, and after image data acquisition is carried out by a data acquisition module 36, the image information is transmitted to a control module 31 for processing and analysis, and then the image information is displayed and is recorded on a display module 32 and stored in a re-storage module 33.
After the experiment is finished, the experimenter can operate the data analysis unit 34 of the multi-physical-field control system 3, and call the experimental process image information in the storage module 33 and the related data in the experimental process to perform data analysis.
As shown in fig. 2, the multi-physical field generating module 2 includes a multi-physical field system power module 21, a microcontroller 22, a multi-physical field generating module 23, a multi-physical field selection output module 24, and a multi-physical field transmission module 25.
The multi-physical-field system power module 21 comprises a direct-current (DC) module 212 for supplying power to a microcontroller, a power preprocessing module 211 for filtering power supplied by the system, a high-voltage adjustable power module 213 for supplying power to the multi-physical-field system, an adjustable radio-frequency power module 214, a spike wave power module 215 and an adjustable constant-current source 216.
The multi-physical-field generating module 23 mainly comprises a high-voltage pulse generating module 231, a radio-frequency generating module 232, a spike wave generating module 233, a direct-current heating module 234 and a liquid nitrogen phase change control module 235, wherein the high-voltage pulse generating module 231 can generate a pulse square wave with adjustable amplitude of 0-5KV, adjustable frequency and adjustable pulse width, the pulse square wave is used for forming a pulsed electromagnetic field, an electric field, a square wave radio-frequency electric field and the like, the radio-frequency generating module 232 is used for generating a radio-frequency electric field and an electromagnetic field of high frequency, the radio-frequency electric field can be optionally used for wireless energy transmission, the radio-frequency electric field can be used for forming a thermal field, and the spike wave generating module 233 is used for realizing an irregular high-frequency radio-frequency field; the direct current heating module 234 is connected with a heating wire to form a thermal field, and the liquid nitrogen phase change control module 235 is used for controlling the liquid nitrogen phase change heat exchange process so as to control a low-temperature thermal field in the scientific experimental device. The microcontroller 22 controls and selects multiple adjustable power supply modules in the multiple physical field system power supply module 21, controls the output power, voltage, current and the like of the multiple physical field generating module 23 corresponding to the physical field by controlling the output of the adjustable power supply, selects the output of at least one physical field by controlling the multiple physical field selecting output module 24, and finally transmits the physical field energy to the scientific experimental device 1 for scientific experimental study by the multiple physical field transmitting module 25.
As shown in fig. 3, the scientific experiment device 1 at least comprises a cell culture dish 11, a multi-physical field electrode array 12, a temperature sensor array 13 (used as a temperature monitoring module), a liquid nitrogen phase change heat exchange channel 14 and a defrosting channel 15.
A plurality of liquid nitrogen phase-change heat exchange channels 14 are arranged on the cell culture dish 11, then organic glass is arranged on the liquid nitrogen phase-change heat exchange channels 14, and a transparent electrode array of ITO materials, namely a multi-physical field electrode array 12, is engraved on the organic glass by adopting a microfluidic technology and is used for transmitting different physical field energy; a micro thermocouple sensor array is distributed near the multi-physical field electrode array 12 and used for collecting the temperature in the overlength of culture in real time, a cell culture medium is designed on the micro thermocouple sensor array and can be suitable for the environment of cell culture, a plurality of defrosting channels 15 are distributed on the side face of the cell culture dish 11 and used for introducing nitrogen for defrosting in the low-temperature freezing process, and the influence of frosting on the observation field of view is prevented. In the working process, the multi-physical field energy transmits radio frequency heat energy, microwave heat energy, high-frequency electric field energy, high-frequency electromagnetic energy and the like to a specific position of cell culture through the multi-physical field electrode array 12, and meanwhile, the temperature sensor array 13 at the corresponding position acquires nearby temperature information in real time; liquid nitrogen flows into the liquid nitrogen phase-change heat exchange channel 14 to perform phase-change heat exchange, absorbs energy of nearby cells to enable the cells to be in a low-temperature state, meanwhile, nitrogen converted after the liquid nitrogen phase-change heat exchange can defrost a low-temperature area through the defrosting channel 15, and after the temperature sensor array 13 collects temperature in real time and feeds the temperature back to the industrial computer 4, the industrial computer 4 accurately controls the temperature and the cooling rate of the low-temperature area in real time.
As shown in fig. 4, the liquid nitrogen phase change control module 235 at least includes a small liquid nitrogen tank 2355, a liquid nitrogen inflow pipe 2352, a nitrogen discharge passage 2351, a negative pressure electromagnetic valve 2353, and a gas three-way valve 2354. In the process that the system needs to be frozen at low temperature, firstly, a negative pressure electromagnetic valve 2353 is started to control the pressure of a nitrogen discharge channel 2351, so that the whole pipeline is in a negative pressure state, liquid nitrogen in a small liquid nitrogen tank 2355 is transmitted to a liquid nitrogen phase-change heat exchange channel 14 through a liquid nitrogen inflow pipeline 2352, phase-change heat exchange is carried out, high-temperature nitrogen is converted into be discharged, when the nitrogen flows out to a gas three-way valve 2354, the nitrogen can be selectively discharged to a defrosting channel 15 to defrost a cell culture dish 11, and the nitrogen can also be directly discharged.
The multi-physical-field scientific experiment system provided by the embodiment adopts a multi-electrode output mode, can transmit the energy of pulse high voltage, a radio frequency module, a spike wave generation module and direct current electric energy, is matched with a corresponding energy transmission device at an output end, can be converted into a multi-path high-frequency electric field, a high-frequency electromagnetic field, a high-temperature thermal field and the like with different waveforms, acts on biological samples of biological tissues and cells, regulates and controls functions of the biological samples, and simultaneously utilizes the liquid nitrogen phase change heat exchange principle, and actively flows into a phase change heat exchange device by adopting a negative pressure control liquid nitrogen outlet so as to form a low-temperature thermal field in the phase change heat exchange device.
The low-temperature field of the system can control the negative pressure degree of the nitrogen discharge channel in real time by accurately controlling the work of the negative pressure electromagnetism, so that the inflow rate of liquid nitrogen is regulated in real time, the heat exchange amount of the liquid nitrogen in the phase-change heat exchange channel is regulated, the temperature of the phase-change heat exchange channel is regulated in real time, meanwhile, the temperature fed back by a nearby temperature sensor is regulated, a control algorithm is regulated, and the temperature reduction process in the scientific experiment process are controlled in real time.
The multi-physical-field energy transmission in the cell culture device adopts a plurality of micro electrode arrays and a plurality of temperature sensor arrays, or adopts a plurality of micro liquid nitrogen heat exchange channels to carry out physical energy transmission, and each energy transmission channel is mutually independent and can be independently controlled, so that the cells in one culture dish are in different temperature and physical-field ranges. Is beneficial to the contrast research of the influence of different physical fields on the cell culture.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A multi-physical field scientific experiment system, comprising:
a multi-physical field generating means for generating at least one form of physical field;
the multi-physical field control system is used for controlling the working modes of the multi-physical fields and monitoring physical parameters and experimental processes in real time; and
A biological assay device configured to carry a biological sample, the biological assay device receiving the physical fields transmitted by the multiple physical field transmission module to regulate a function of the biological sample; the multi-physical field transmission module comprises an electric signal physical field transmission channel and a low-temperature field transmission channel;
The multi-physical-field control system comprises a control module, a storage module, a data acquisition module, a data analysis unit and an input and display unit, and is used for controlling the work of the physical-field generating device, acquiring experimental parameters and biological experimental process information in real time, and storing the acquired information into the storage module so as to later call the data analysis unit for data analysis;
the system comprises a biological experimental device, a control module, an image acquisition module and a control module, wherein the biological experimental device is used for acquiring the biological sample in real time;
The biological experiment device comprises a probe, a cavity and a platform which act on an experiment sample; the device is used for accurately transmitting the multiple physical fields to a biological sample for experimental study;
A plurality of temperature sensors are also included;
The multi-physical field generating apparatus further includes: the liquid nitrogen phase change control module adopts the arrangement of control of a plurality of groups of micro liquid nitrogen phase change channels, and comprises a liquid nitrogen liquid inlet channel, a liquid nitrogen outlet channel, a negative pressure regulating and controlling proportional valve, a gas three-way valve and a liquid nitrogen bottle, wherein the liquid nitrogen phase change control module is connected with the micro phase change heat exchange channels in the biological experiment device in the working process, the negative pressure regulating and controlling proportional valve regulates and controls the negative pressure quantity of the nitrogen outlet channel, adjusts the flow rate of the liquid nitrogen liquid inlet channel and controls the gas-liquid ratio of the micro phase change heat exchange channels in the biological experiment device, so that the temperature of the phase change heat exchange channels is controlled;
the cell culture device in the biological experiment device at least comprises a cell culture dish, a multi-physical field electrode array, a temperature sensor array, a multi-path liquid nitrogen phase change heat exchange channel and a defrosting channel; the multi-channel liquid nitrogen phase change heat exchange channels are uniformly distributed on the cell culture dish and used for generating low temperature through phase change heat of liquid nitrogen, then heat conducting glass is designed on the heat conducting channel, meanwhile, a plurality of physical field electrode arrays are processed and designed on the glass and used for transmitting different forms of physical field energy, and meanwhile, a temperature sensor array is designed at a specific position near the electrodes and used for collecting the temperature in the cell culture process in real time; a defrosting channel is designed on the side surface of the culture dish;
The multi-physical field electrode array is made of transparent conductive glass ITO material, is engraved on glass by utilizing a microscale processing technology, and each electrode is mutually independent and is led out by a micro-fine guide wire to transmit various physical field energies;
The device comprises a cell culture device, and is characterized by further comprising a liquid nitrogen phase-change heat exchange control module, wherein a nitrogen discharge channel of the liquid nitrogen phase-change heat exchange control module is provided with a gas three-way valve, and the nitrogen after heat exchange is circulated into the cell culture device for defrosting by controlling the gas three-way valve.
2. The multi-physical field scientific experiment system according to claim 1, wherein the multi-physical field generating device comprises an electric field, a magnetic field, an electromagnetic field, a high temperature thermal field and a low temperature thermal field generating module.
3. The multi-physical field scientific experiment system of claim 2, wherein: the electric field and the magnetic field mainly comprise a radio frequency electric field, a radio frequency magnetic field, a microwave magnetic field, a pulse electric field and a pulse magnetic field.
4. The multi-physical field scientific experiment system of claim 1, wherein: a cryogenic transfer module is included.
5. The multi-physical field scientific experiment system of claim 1, wherein: a plurality of electrodes for transmitting electric, magnetic, thermal fields are also included.
6. The multi-physical field scientific experiment system of claim 4, wherein: the low temperature source of the low temperature transmission module is from liquid nitrogen.
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| CN103674678A (en) * | 2013-12-17 | 2014-03-26 | 电子科技大学 | Microchannel chip and device for low-temperature preservation of biomaterial |
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