CN117269028A - Method, device, medium and equipment for judging insufficient dosage of hemolytic agent - Google Patents
Method, device, medium and equipment for judging insufficient dosage of hemolytic agent Download PDFInfo
- Publication number
- CN117269028A CN117269028A CN202311229875.4A CN202311229875A CN117269028A CN 117269028 A CN117269028 A CN 117269028A CN 202311229875 A CN202311229875 A CN 202311229875A CN 117269028 A CN117269028 A CN 117269028A
- Authority
- CN
- China
- Prior art keywords
- particle
- intensity
- signal intensity
- blood cell
- hemolytic agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1488—Methods for deciding
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a method, a device, a medium and equipment for judging the insufficient dosage of a hemolytic agent. If the number of the local maxima of the particle number is greater than 1, judging that the current hemolytic agent dosage is seriously insufficient; if the number of the local maxima of the particle number is equal to 1, judging whether the dosage of the hemolytic agent is slightly insufficient or sufficient by calculating a correlation coefficient. Therefore, the invention can monitor the dosage of the hemolytic agent based on the local maximum value of the particle number and the correlation coefficient, can lead the instrument structure to be more simplified, and is accurate enough because the hemolytic agent is not influenced by interference factors such as bubbles and the like.
Description
Technical Field
The invention relates to the technical field of hemolytic agents, in particular to a method, a device, a medium and equipment for judging insufficient dosage of a hemolytic agent.
Background
Blood cell analyzers can count various blood cells in blood, for example, in one scenario, when 5LDS hemolysis agent is mixed with fresh blood sample, red blood cells are lysed and white blood cells are stained, then 5LHS hemolysis agent is added, and 5LHS alkaline hemolysis agent baryoses white blood cells except BASO, so that BASO cells are obviously differentiated from other cells in volume.
The detection principle is shown in figure 1, under the wrapping of sheath liquid, cells to be detected are arranged in a single row and flow into the flow chamber at a constant speed, three scattering lights with different angles are generated under the irradiation of laser beams, and the size of the scattering lights generated by the cells under the irradiation of the laser beams is related to the refractive indexes of the cell size, cell membranes and the complexity degree of the internal organs of the cells; the scattered light signals are finally converted into electric pulse signals, according to the acquired electric pulse data, scatter diagram distribution of the white blood cells under the three-dimensional signals can be obtained, and finally, the classification result of the white blood cells is obtained according to the scatter diagram of the white blood cells.
When the blood cell analyzer is measuring a sample, for example, if the 5LHS hemolysis reagent required in the above-mentioned scenario is insufficient, the white blood cell scattergram may be abnormal, thereby causing abnormal classification of white blood cells, and finally, the reported cell count result is inaccurate. The traditional method for detecting the residual quantity of the hemolysis agent is to detect the liquid level of the hemolysis agent by a sensor to judge the residual quantity of the reagent. However, the method needs hardware support, the principle is complex, the device is large in size, and the whole detection device cannot work normally once a certain component has a problem due to the fact that the number of components is large. In addition, the sensor detection is greatly affected by interference factors, such as most common bubble interference, and when bubbles exist, the hemolysis agent is possibly misjudged to be insufficient. Therefore, a simple, quick and effective method is needed to alert in the case of insufficient amounts of hemolysis agent.
Disclosure of Invention
Based on the above, it is necessary to provide a method, a device, a medium and a device for judging the insufficient dosage of the hemolytic agent, so as to solve the problem of inaccurate detection of the leucocytes caused by the insufficient dosage of the hemolytic agent.
A method of determining a low dosage of a haemolytic agent, the method comprising:
acquiring a pulse signal set of a blood cell sample to be detected under a preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution condition of the signal intensity; the particle distribution condition is used for indicating the particle numbers of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after the current hemolytic agent treatment;
calculating the particle number change rate of each type of signal intensity in the particle distribution condition to obtain a change rate condition;
searching for a falling zero crossing in the change rate situation, and determining a local maximum of the particle count in the particle distribution situation based on the searched falling zero crossing;
if the number of the local maxima of the particle number is greater than 1, judging that the current dosage of the hemolytic agent is seriously insufficient;
if the number of the local maxima of the particle number is equal to 1, calculating an intensity mean value and an intensity standard deviation of the signal intensity under different scattering angles, and calculating a correlation coefficient based on the intensity mean value and the intensity standard deviation;
if the correlation coefficient is smaller than a preset threshold value, judging that the current dosage of the hemolytic agent is slightly insufficient; and if the correlation coefficient is larger than or equal to a preset threshold value, judging that the current dosage of the hemolytic agent is sufficient.
In one embodiment, the calculation formula of the particle number change rate is:
S i ＝La i+1 -La i ，(i∈1,2,3,…N-1)
in the above, S i Particle count change rate indicating i-th signal intensity, la i+1 Particle count of blood cell particles indicating i+1st class signal intensity, la i The number of blood cell particles indicating the i-th class of signal intensity, and N indicates the total class number of signal intensities.
In one embodiment, the searching for the falling zero crossing in the rate of change case and determining the local maxima of the population in the population distribution case based on the searched falling zero crossing includes:
searching all descending zero crossing points in the change rate condition, and acquiring the particle number corresponding to each descending zero crossing point in the particle distribution condition to serve as the local maximum value of the particle number.
In one embodiment, the intensity average value has a calculation formula:
in the above-mentioned method, the step of,indicating the intensity mean value corresponding to the characteristic value k, +.>Indicating the signal intensity of an a-th cell in a blood cell sample to be detected under a preset scattering angle corresponding to a characteristic value k, wherein A indicates the total number of cells;
the calculation formula of the intensity standard deviation is as follows:
in the above-mentioned method, the step of,and indicating the standard deviation of the intensity corresponding to the characteristic value k.
In one embodiment, the correlation coefficient is calculated by the following formula:
in the above-mentioned method, the step of,the correlation coefficient is characterized by a characteristic value of 1 or 2, which is any two of the signal intensity of low-angle scattered light, the signal intensity of medium-angle scattered light, and the signal intensity of high-angle scattered light.
In one embodiment, after the counting the particle distribution of the signal intensity, the method further includes:
filtering the particle distribution condition; the formula of the filtering process is as follows:
in the above, F (Ful) i ) Ful for particle distribution after the filtering treatment i For the particle distribution before the filtering process, i indicates the i-th type signal intensity, N indicates the total type number of the signal intensities, u indicates the mean value, and σ indicates the standard deviation.
A device for determining a low dosage of a hemolyzing agent, the device comprising:
the particle distribution condition determining module is used for acquiring a pulse signal set of a blood cell sample to be detected under a preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution condition of the signal intensity; the particle distribution condition is used for indicating the particle numbers of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after the current hemolytic agent treatment;
the extreme point determining module is used for calculating the particle number change rate of each type of signal intensity in the particle distribution condition so as to obtain the change rate condition; searching for a falling zero crossing in the change rate situation, and determining a local maximum of the particle count in the particle distribution situation based on the searched falling zero crossing;
the judging module is used for judging that the current dosage of the hemolytic agent is seriously insufficient if the number of the local maxima of the particle number is larger than 1; if the number of the local maxima of the particle number is equal to 1, calculating an intensity mean value and an intensity standard deviation of the signal intensity under different scattering angles, and calculating a correlation coefficient based on the intensity mean value and the intensity standard deviation; if the correlation coefficient is smaller than a preset threshold value, judging that the current dosage of the hemolytic agent is slightly insufficient; and if the correlation coefficient is larger than or equal to a preset threshold value, judging that the current dosage of the hemolytic agent is sufficient.
A computer-readable storage medium storing a computer program which, when executed by a processor, causes the processor to execute the steps of the above-described hemolysis agent underdosage determination method.
A hemolysis agent dosage insufficiency judging device comprises a memory and a processor, wherein the memory stores a computer program, and the computer program, when executed by the processor, causes the processor to execute the steps of the hemolysis agent dosage insufficiency judging method.
The invention provides a method, a device, a medium and equipment for judging the insufficient dosage of a hemolytic agent, which are used for acquiring a pulse signal set of a blood cell sample to be tested under a preset scattering angle and counting the particle distribution condition of signal intensity, further calculating the particle number change rate of each type of signal intensity in the particle distribution condition, searching a descending zero crossing point in the change rate condition, and determining the local maximum value of the particle number in the particle distribution condition based on the searched descending zero crossing point so as to judge whether the current dosage of the hemolytic agent is insufficient. If the number of the local maxima of the particle number is greater than 1, judging that the current hemolytic agent dosage is seriously insufficient; if the number of the local maxima of the particle number is equal to 1, judging whether the dosage of the hemolytic agent is slightly insufficient or sufficient by calculating a correlation coefficient. Therefore, the invention can monitor the dosage of the hemolytic agent based on the local maximum value of the particle number and the correlation coefficient, can reduce the cost required by the detection of the instrument, ensures that the instrument structure is more simplified, and is accurate enough because the instrument is not influenced by interference factors such as bubbles.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Wherein:
FIG. 1 is a schematic diagram of the principle of white blood cell monitoring;
FIG. 2 is a flow chart of a method for determining insufficient dosage of a hemolyzing agent;
FIG. 3 is a schematic diagram of the generation of three different angles of scattered light;
FIG. 4 shows a signal intensity scatter plot and particle distribution of white blood cells in the case of severe hemolysis agent deficiency;
FIG. 5 is a schematic diagram of extreme points in the case of the particle distribution of FIG. 4;
FIG. 6 shows the correlation coefficients of the blood cell samples (a), (b), and (c) to be tested;
FIG. 7 is a schematic structural view of a device for judging insufficient dosage of a hemolyzing agent;
FIG. 8 is a block diagram showing the constitution of a judging device for insufficient dose of a hemolyzing agent.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The terms first, second and the like in the description and in the claims of the present application and in the above-described figures, are used for distinguishing between different objects and not for describing a particular sequential order. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
As shown in fig. 2, fig. 2 is a flow chart of a method for determining a dosage insufficiency of a hemolytic agent according to an embodiment, and the method for determining a dosage insufficiency of a hemolytic agent according to the embodiment includes the following steps:
s201, acquiring a pulse signal set of a blood cell sample to be detected under a preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution condition of the signal intensity.
The particle distribution condition is used for indicating the particle numbers of blood cell particles with different signal intensities, and the blood cell sample to be tested is the blood cell sample obtained after the current hemolytic agent treatment.
In one scenario, for example, a sample of blood cells to be tested is first treated with a sufficient amount of 5LDS hemolytic agent, the treated red blood cells are lysed and the white blood cells are stained, then a current 5LHS hemolytic agent is added, and the 5LHS basic hemolytic agent barks white blood cells other than BASO, so that the BASO cells are significantly different from the rest of the cells in volume, and white blood cells and red blood cell fragments can be obtained. Under the wrapping of the sheath fluid, the cells are arranged in a single row, flow into the flow chamber at a constant speed, and under the irradiation of the laser beam, three different angles of scattered light are generated, referring to fig. 3. The generated scattered light includes low-angle scattered light, i.e., scattered light in a forward low-angle region, medium-angle scattered light, i.e., scattered light in a forward medium-angle region, and high-angle scattered light, i.e., scattered light in a lateral high-angle region. The low angle scattered light can reflect the cell size, the medium angle scattered light can reflect the internal fine structure and particulate matter of the cell, and the high angle forward scattered light can also reflect the internal fine structure and particulate matter of the cell. The diaphragm of the receiving part is used for determining whether scattered light exists, and the first receiver receives the medium angle scattered light emitted from the flow chamber and converts the medium angle scattered light into medium angle pulse signals to form a pulse signal set corresponding to the medium angle; the second receiver receives the high-angle scattered light emitted from the flow chamber and converts the high-angle scattered light into high-angle pulse signals to form a pulse signal set corresponding to the high angle; the third receiver receives the low-angle scattered light emitted from the flow chamber and converts the low-angle scattered light into low-angle pulse signals to form a pulse signal set corresponding to the low angle.
Further, the signal strength of each pulse signal in the pulse signal set is identified by the existing pulse identification algorithm, such as a threshold detection algorithm, or a pulse identification algorithm such as an energy threshold algorithm. Summarizing the signal intensity of all pulse signals to obtain Rp a (a.epsilon.1, 2,3, … A) represents the signal strength of the pulse signal of the a-th white blood cell in the sample, where A represents the total number of pulse signals.
Further, based on these signal intensities, a signal intensity scatter diagram of the white blood cells under the three-dimensional signal can be obtained. Illustratively, as shown in fig. 4 (a), the Y-axis-LS in this figure represents the signal intensity of low angle scattered light, and the X-axis-MS represents the signal intensity of medium angle scattered light. Of course, the scatter plot may also be constructed based on scattered light at other angles.
Further, counting the particle distribution of the signal intensities, which is indicative of the particle numbers of the blood cell particles of different signal intensities, wherein La i (i=1, 2,3, … N) indicates the number of particles of the i-th class of signal intensity, N being the total class of signal intensity. For example, the particle distribution shown in fig. 4 (b) can be obtained by mapping the scatter diagram in fig. 4 (a) in the LS direction, corresponding to fig. 4.
In one embodiment, the particle distribution is also filtered; the formula of the filtering process is as follows:
in the above, F (Ful) i ) Ful for particle distribution after the filtering treatment i For the particle distribution before the filtering process, i indicates the i-th type signal intensity, N indicates the total type number of the signal intensities, u indicates the mean value, and σ indicates the standard deviation.
After the filtering treatment, noise in the particle distribution condition can be effectively removed, so that the overall distribution is smoother.
S202, calculating the particle number change rate of each type of signal intensity in the particle distribution condition to obtain the change rate condition.
Based on the change rate, the trend of the particle number change at each signal intensity in the particle distribution can be known.
In one embodiment, the calculation formula for the particle count change rate of the i-th type signal intensity is:
S i ＝La i+1 -La i ，(i∈1,2,3,…N-1)
in the above, S i Particle count change rate indicating i-th signal intensity, la i+1 Particle count of blood cell particles indicating i+1st class signal intensity, la i The number of blood cell particles indicating the i-th class of signal intensity, and N indicates the total class number of signal intensities.
S203, searching for a falling zero crossing point in the change rate situation, and determining a local maximum value of the particle count in the particle distribution situation based on the searched falling zero crossing point.
Wherein, in the case of the rate of change, the number of particles in the left partial range of the falling zero-crossing point is greater than 0, which indicates the signal intensity in the corresponding particle distribution situation is always increasing; the number of particles representing the signal intensity in the corresponding particle distribution situation is always decreasing when the right local range of the falling zero crossing point is smaller than 0, and the falling zero crossing point can be found in the change rate situation based on the conditions. Alternatively, the falling zero-crossing is defined as the rate of change S of the number of particles for an i-th type signal intensity i The following condition is determined as a falling zero-crossing point:
based on these detected falling zero crossings, a local maximum of the population can then be determined in the case of a particle distribution.
In one embodiment, the local maxima of the population are determined by: searching all descending zero crossing points in the change rate condition, and acquiring the particle number corresponding to each descending zero crossing point in the particle distribution condition to serve as a local maximum value of the particle number. Illustratively, in fig. 5, the corresponding particle count is determined A, B, C as the local maximum of the particle count.
S204, comparing the number of the local maxima of the particle number with the size of 1. If the number of local maxima of the particle number is greater than 1, S205 is executed to determine that the current hemolytic agent dose is severely insufficient. If the number of local maxima of the population is equal to 1, then S206 is performed.
Illustratively, as shown in fig. 5, if the number of local maxima of the particle number is 3 and greater than 1, the current hemolytic agent dosage is determined to be severely insufficient.
This is because, if the required hemolysis reagent is severely insufficient, the white blood cell bare cells other than the cells to be detected cannot be completely nucleated, many abnormal points (points within the range of the square) other than the cells to be detected appear in the scatter diagram of fig. 4 (a), and after mapping, a plurality of local maxima of the particle count appear in the particle distribution situation of fig. 4 (b) (redundant local maxima of the particle count appear in the elliptical region).
Otherwise, if the number of the local maxima of the particle number is equal to 1, it indicates that the current hemolytic agent dosage is not seriously insufficient, and the current hemolytic agent dosage can be further accurately determined through the subsequent steps.
S206, calculating the intensity mean value and the intensity standard deviation of the signal intensity under different scattering angles, and calculating the correlation coefficient based on the intensity mean value and the intensity standard deviation.
The calculation formula of the intensity mean value is as follows:
in the above-mentioned method, the step of,indicating the intensity mean value corresponding to the characteristic value k, +.>Indicating the signal intensity of an a-th cell in a blood cell sample to be detected under a preset scattering angle corresponding to a characteristic value k, wherein A indicates the total number of cells;
the calculation formula of the intensity standard deviation is as follows:
in the above-mentioned method, the step of,and indicating the standard deviation of the intensity corresponding to the characteristic value k.
Wherein, the calculation formula of the correlation coefficient is:
in the above-mentioned method, the step of,the characteristic values 1 and 2 are any two of the signal intensity of the low angle scattered light, the signal intensity of the medium angle scattered light, and the signal intensity of the high angle scattered light, as the correlation coefficient. The magnitude of the correlation coefficient reflects the correlation of the signal intensity of the particles under different scattering angles, and the larger the value is, the stronger the correlation is.
For example, referring to fig. 6, fig. 6 shows correlation coefficients of blood cell samples (a), (b), and (c), where the selected characteristic values 1 and 2 are signal intensities of low-angle scattered light and signal intensities of medium-angle scattered light. The correlation coefficient of the blood cell sample (a) to be measured was 92.234. The correlation coefficient of the blood cell sample (b) to be measured was 38.114. The correlation coefficient of the blood cell sample (c) to be measured was 10.454.
S207, judging whether the correlation coefficient is smaller than a preset threshold value. If the correlation coefficient is smaller than the preset threshold, S208 is executed to determine that the current hemolytic agent dose is slightly insufficient. If the correlation coefficient is greater than or equal to the preset threshold, S209 is executed to determine that the current hemolytic agent dosage is sufficient.
Alternatively, the threshold is set to 60, so that it can be determined that the remaining amount of the hemolytic agent used for the blood cell sample to be tested of fig. 6 (a) is sufficient; the remaining amount of the hemolytic agent used in the blood cell samples to be tested in FIGS. 6 (b) and (c) is slightly insufficient. And the hemolytic agent dose of fig. 6 (c) is less than the hemolytic agent dose of fig. 6 (b). This is because, on the premise that the hemolysis reagent is sufficient, the bare white blood cells other than the cells to be detected are completely nucleated, a small number of abnormal points other than the cells to be detected do not appear in the scatter diagram, only 1 obvious cluster appears, and under the condition, the correlation between the characteristic values is strong and the correlation coefficient is also large. With the gradual decrease of the hemolysis reagent, the bare white blood cells except the cells to be detected cannot be completely nucleated, a small number of abnormal points except the cells to be detected appear in the scatter diagram, clusters are gradually dispersed, and as shown in fig. 6 (b) and (c), the correlation between the characteristic values is weakened and the correlation sparsity is also reduced.
According to the method for judging the insufficient dosage of the hemolytic agent, the pulse signal set of the blood cell sample to be tested under the preset scattering angle is obtained, the particle distribution condition of the signal intensity is counted, the particle number change rate of each type of signal intensity in the particle distribution condition is calculated, the descending zero crossing point in the change rate condition is searched, the local maximum value of the particle number is determined in the particle distribution condition based on the searched descending zero crossing point, and therefore whether the current dosage of the hemolytic agent is insufficient or not is judged. If the number of the local maxima of the particle number is greater than 1, judging that the current hemolytic agent dosage is seriously insufficient; if the number of the local maxima of the particle number is equal to 1, judging whether the dosage of the hemolytic agent is slightly insufficient or sufficient by calculating a correlation coefficient. Therefore, the invention can monitor the dosage of the hemolytic agent based on the local maximum value of the particle number and the correlation coefficient, can reduce the cost required by the detection of the instrument, ensures that the instrument structure is more simplified, and is accurate enough because the instrument is not influenced by interference factors such as bubbles.
In one embodiment, as shown in fig. 7, a device for determining a dose deficiency of a hemolysis agent is provided, the device comprising:
the particle distribution determining module 701 is configured to obtain a pulse signal set of a blood cell sample to be tested at a preset scattering angle, identify a signal intensity of each pulse signal in the pulse signal set, and count a particle distribution of the signal intensity; the particle distribution condition is used for indicating the particle numbers of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after the current hemolytic agent treatment;
the extreme point determining module 702 is configured to calculate a particle number change rate of each type of signal intensity in the particle distribution situation, so as to obtain a change rate situation; searching for a falling zero crossing in the case of a change rate, and determining a local maximum of the particle count in the case of particle distribution based on the searched falling zero crossing;
a judging module 703, configured to judge that the current dose of the hemolytic agent is seriously insufficient if the number of the local maxima of the particle number is greater than 1; if the number of the local maxima of the particle number is equal to 1, calculating an intensity mean value and an intensity standard deviation of the signal intensity under different scattering angles, and calculating a correlation coefficient based on the intensity mean value and the intensity standard deviation; if the correlation coefficient is smaller than a preset threshold value, judging that the current dosage of the hemolytic agent is slightly insufficient; if the correlation coefficient is greater than or equal to a preset threshold value, judging that the current dosage of the hemolytic agent is sufficient.
In one embodiment, the calculation formula of the particle number change rate is:
S i ＝La i+1 -La i ，(i∈1,2,3,…N-1)
in the above, S i Particle count change rate indicating i-th signal intensity, la i+1 Particle count of blood cell particles indicating i+1st class signal intensity, la i The number of blood cell particles indicating the i-th class of signal intensity, and N indicates the total class number of signal intensities.
In one embodiment, the extremum point determining module 702 is specifically configured to:
searching all descending zero crossing points in the change rate condition, and acquiring the particle number corresponding to each descending zero crossing point in the particle distribution condition to serve as a local maximum value of the particle number.
In one embodiment, the intensity average is calculated as:
in the above-mentioned method, the step of,indicating the intensity mean value corresponding to the characteristic value k, +.>Indicating the signal intensity of an a-th cell in a blood cell sample to be detected under a preset scattering angle corresponding to a characteristic value k, wherein A indicates the total number of cells;
the calculation formula of the standard deviation of the intensity is:
in the above-mentioned method, the step of,and indicating the standard deviation of the intensity corresponding to the characteristic value k.
In one embodiment, the correlation coefficient is calculated by the formula:
in the above-mentioned method, the step of,the characteristic values 1 and 2 are any two of the signal intensity of the low angle scattered light, the signal intensity of the medium angle scattered light, and the signal intensity of the high angle scattered light, as the correlation coefficient.
In one embodiment, the means for determining a low dose of a haemolytic agent is further adapted to:
carrying out filtering treatment on the particle distribution condition; the formula of the filtering process is as follows:
in the above, F (Ful) i ) Ful for particle distribution after the filtering treatment i For the particle distribution before the filtering process, i indicates the i-th type signal intensity, N indicates the total type number of the signal intensities, u indicates the mean value, and σ indicates the standard deviation.
FIG. 8 is a diagram showing an internal structure of a judgment device for a shortage of a hemolytic agent dose in one embodiment. As shown in fig. 8, the apparatus for determining the insufficient dose of the hemolysis agent includes a processor, a memory, and a network interface connected via a system bus. The memory includes a nonvolatile storage medium and an internal memory. The nonvolatile storage medium of the hemolysis agent dosage insufficiency judging device stores an operating system, and can also store a computer program, and when the computer program is executed by a processor, the processor can realize the hemolysis agent dosage insufficiency judging method. The internal memory may also store a computer program which, when executed by the processor, causes the processor to perform a method for determining that the dosage of the hemolyzing agent is insufficient. It will be appreciated by those skilled in the art that the structure shown in fig. 8 is merely a block diagram of a part of the structure related to the present application and does not constitute a limitation of the apparatus for determining that the hemolysis agent dose is insufficient to which the present application is applied, and that the specific apparatus for determining that the hemolysis agent dose is insufficient may include more or less components than those shown in the drawings, or may combine some components, or may have different arrangements of components.
A computer readable storage medium storing a computer program which when executed by a processor performs the steps of: acquiring a pulse signal set of a blood cell sample to be detected under a preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution condition of the signal intensity; the particle distribution condition is used for indicating the particle numbers of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after the current hemolytic agent treatment; calculating the particle number change rate of each type of signal intensity in the particle distribution condition to obtain a change rate condition; searching for a falling zero crossing in the case of a change rate, and determining a local maximum of the particle count in the case of particle distribution based on the searched falling zero crossing; if the number of the local maxima of the particle number is greater than 1, judging that the current dosage of the hemolytic agent is seriously insufficient; if the number of the local maxima of the particle number is equal to 1, calculating an intensity mean value and an intensity standard deviation of the signal intensity under different scattering angles, and calculating a correlation coefficient based on the intensity mean value and the intensity standard deviation; if the correlation coefficient is smaller than a preset threshold value, judging that the current dosage of the hemolytic agent is slightly insufficient; if the correlation coefficient is greater than or equal to a preset threshold value, judging that the current dosage of the hemolytic agent is sufficient.
A device for determining a low dosage of a haemolytic agent comprising a memory, a processor and a computer program stored in the memory and executable on the processor, the processor implementing the following steps when executing the computer program: acquiring a pulse signal set of a blood cell sample to be detected under a preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution condition of the signal intensity; the particle distribution condition is used for indicating the particle numbers of blood cell particles with different signal intensities, and the blood cell sample to be tested is a blood cell sample obtained after the current hemolytic agent treatment; calculating the particle number change rate of each type of signal intensity in the particle distribution condition to obtain a change rate condition; searching for a falling zero crossing in the case of a change rate, and determining a local maximum of the particle count in the case of particle distribution based on the searched falling zero crossing; if the number of the local maxima of the particle number is greater than 1, judging that the current dosage of the hemolytic agent is seriously insufficient; if the number of the local maxima of the particle number is equal to 1, calculating an intensity mean value and an intensity standard deviation of the signal intensity under different scattering angles, and calculating a correlation coefficient based on the intensity mean value and the intensity standard deviation; if the correlation coefficient is smaller than a preset threshold value, judging that the current dosage of the hemolytic agent is slightly insufficient; if the correlation coefficient is greater than or equal to a preset threshold value, judging that the current dosage of the hemolytic agent is sufficient.
It should be noted that the above method, device, apparatus and computer readable storage medium for determining a dose deficiency of a hemolytic agent belong to a general inventive concept, and the content in the embodiments of the method, device, apparatus and computer readable storage medium for determining a dose deficiency of a hemolytic agent may be mutually applicable.
Those skilled in the art will appreciate that implementing all or part of the above-described methods in accordance with the embodiments may be accomplished by way of a computer program stored in a non-transitory computer-readable storage medium, which when executed, may comprise the steps of the embodiments of the methods described above. Any reference to memory, storage, database, or other medium used in the various embodiments provided herein may include non-volatile and/or volatile memory. The nonvolatile memory can include Read Only Memory (ROM), programmable ROM (PROM), electrically Programmable ROM (EPROM), electrically Erasable Programmable ROM (EEPROM), or flash memory. Volatile memory can include Random Access Memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in a variety of forms such as Static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double Data Rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous Link DRAM (SLDRAM), memory bus direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), among others.
The technical features of the above embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The foregoing examples represent only a few embodiments of the present application, which are described in more detail and are not thereby to be construed as limiting the scope of the present application. It should be noted that it would be apparent to those skilled in the art that various modifications and improvements could be made without departing from the spirit of the present application, which would be within the scope of the present application. Accordingly, the scope of protection of the present application is to be determined by the claims appended hereto.
Claims (9)
1. A method for determining a low dosage of a hemolytic agent, the method comprising:
acquiring a pulse signal set of a blood cell sample to be detected under a preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution condition of the signal intensity; the blood cell sample to be detected is a blood cell sample obtained after the current hemolytic agent treatment, and the particle distribution condition is used for indicating the particle numbers of blood cell particles with different signal intensities;
calculating the particle number change rate of each type of signal intensity in the particle distribution condition to obtain a change rate condition;
searching for a falling zero crossing in the change rate situation, and determining a local maximum of the particle count in the particle distribution situation based on the searched falling zero crossing;
if the number of the local maxima of the particle number is greater than 1, judging that the current dosage of the hemolytic agent is seriously insufficient;
if the number of the local maxima of the particle number is equal to 1, calculating an intensity mean value and an intensity standard deviation of the signal intensity under different scattering angles, and calculating a correlation coefficient based on the intensity mean value and the intensity standard deviation;
if the correlation coefficient is smaller than a preset threshold value, judging that the current dosage of the hemolytic agent is slightly insufficient; and if the correlation coefficient is larger than or equal to a preset threshold value, judging that the current dosage of the hemolytic agent is sufficient.
2. The method of claim 1, wherein the rate of change of the number of particles is calculated by the formula:
S i ＝La i+1 -La i ，(i∈1,2,3,…N-1)
in the above, S i Particle count change rate indicating i-th signal intensity, la i+1 Particle count of blood cell particles indicating i+1st class signal intensity, la i The number of blood cell particles indicating the i-th class of signal intensity, and N indicates the total class number of signal intensities.
3. The method of claim 1, wherein said searching for a falling zero crossing in said rate of change case and determining a local maximum of particle count in said particle distribution case based on said searched falling zero crossing comprises:
searching all descending zero crossing points in the change rate condition, and acquiring the particle number corresponding to each descending zero crossing point in the particle distribution condition to serve as the local maximum value of the particle number.
4. The method of claim 1, wherein the intensity average is calculated by the formula:
in the above-mentioned method, the step of,indicating the intensity mean value corresponding to the characteristic value k, +.>Indicating the signal intensity of an a-th cell in a blood cell sample to be detected under a preset scattering angle corresponding to a characteristic value k, wherein A indicates the total number of cells;
the calculation formula of the intensity standard deviation is as follows:
in the above-mentioned method, the step of,and indicating the standard deviation of the intensity corresponding to the characteristic value k.
5. The method of claim 4, wherein the correlation coefficient is calculated by the formula:
in the above-mentioned method, the step of,the correlation coefficient is characterized by a characteristic value of 1 or 2, which is any two of the signal intensity of low-angle scattered light, the signal intensity of medium-angle scattered light, and the signal intensity of high-angle scattered light.
6. The method of claim 1, further comprising, after the counting of the particle distribution of the signal intensity:
filtering the particle distribution condition; the formula of the filtering process is as follows:
in the above, F (Ful) i ) Ful for particle distribution after the filtering treatment i For the particle distribution before the filtering process, i indicates the i-th type signal intensity, N indicates the total type number of the signal intensities, u indicates the mean value, and σ indicates the standard deviation.
7. A device for determining a low dosage of a hemolyzing agent, the device comprising:
the particle distribution condition determining module is used for acquiring a pulse signal set of a blood cell sample to be detected under a preset scattering angle, identifying the signal intensity of each pulse signal in the pulse signal set, and counting the particle distribution condition of the signal intensity; the blood cell sample to be detected is a blood cell sample obtained after the current hemolytic agent treatment, and the particle distribution condition is used for indicating the particle numbers of blood cell particles with different signal intensities;
the extreme point determining module is used for calculating the particle number change rate of each type of signal intensity in the particle distribution condition so as to obtain the change rate condition; searching for a falling zero crossing in the change rate situation, and determining a local maximum of the particle count in the particle distribution situation based on the searched falling zero crossing;
the judging module is used for judging that the current dosage of the hemolytic agent is seriously insufficient if the number of the local maxima of the particle number is larger than 1; if the number of the local maxima of the particle number is equal to 1, calculating an intensity mean value and an intensity standard deviation of the signal intensity under different scattering angles, and calculating a correlation coefficient based on the intensity mean value and the intensity standard deviation; if the correlation coefficient is smaller than a preset threshold value, judging that the current dosage of the hemolytic agent is slightly insufficient; and if the correlation coefficient is larger than or equal to a preset threshold value, judging that the current dosage of the hemolytic agent is sufficient.
8. A computer readable storage medium, characterized in that a computer program is stored, which, when being executed by a processor, causes the processor to perform the steps of the method according to any of claims 1 to 6.
9. A device for determining a low dosage of a haemolytic agent, comprising a memory and a processor, the memory storing a computer program which, when executed by the processor, causes the processor to perform the steps of the method according to any of claims 1 to 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311229875.4A CN117269028A (en) | 2023-09-21 | 2023-09-21 | Method, device, medium and equipment for judging insufficient dosage of hemolytic agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311229875.4A CN117269028A (en) | 2023-09-21 | 2023-09-21 | Method, device, medium and equipment for judging insufficient dosage of hemolytic agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117269028A true CN117269028A (en) | 2023-12-22 |
Family
ID=89220887
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311229875.4A Pending CN117269028A (en) | 2023-09-21 | 2023-09-21 | Method, device, medium and equipment for judging insufficient dosage of hemolytic agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117269028A (en) |
-
2023
- 2023-09-21 CN CN202311229875.4A patent/CN117269028A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10627332B2 (en) | Cell analyzer and sorting method therefor | |
| Brummitt et al. | The determination of a reference range for new platelet parameters produced by the Bayer ADVIATM120 full blood count analyser | |
| US12046333B2 (en) | Blood cell analysis method and blood cell analyzer | |
| JP4809892B2 (en) | Method for calibrating a device for analyzing characteristic parameters related to blood concentration, such as erythrocyte sedimentation rate and / or erythrocyte aggregation rate | |
| US11125687B2 (en) | Method and device for identifying fragmented red blood cells, blood cell analyzer and analysis method | |
| EP0874983A1 (en) | Method and apparatus for determination of hemoglobin content of individual red blood cells | |
| US20210164885A1 (en) | Method for detecting a blood sample, blood cell analyzer, and storage medium | |
| CN117269028A (en) | Method, device, medium and equipment for judging insufficient dosage of hemolytic agent | |
| WO2023015606A1 (en) | Total phosphorus measurement method and system for high-turbidity sample, automatic water quality monitoring station, device, and storage medium | |
| CN117191677A (en) | Method, device, medium and equipment for judging insufficient dosage of hemolytic agent | |
| CN117191675A (en) | Method, device, medium and equipment for judging insufficient dosage of hemolytic agent | |
| US4206504A (en) | Method of analyzing a measuring liquid as a function of a preparatory state of a sample contained in the measuring liquid | |
| CN117368076A (en) | Method, device, medium and equipment for judging insufficient dosage of hemolytic agent | |
| CN117191676A (en) | Method, device, medium and equipment for judging insufficient dosage of hemolytic agent | |
| US4933884A (en) | Method of and apparatus for determining threshold values for the analysis of particles contained in a liquid | |
| CN111812070B (en) | Nuclear left shift and value range determining method and device and cell analyzer | |
| CN117388154A (en) | Leukocyte classification method, device, medium and apparatus | |
| Onoz et al. | Assessment of outliers in statistical data analysis | |
| CN114674729B (en) | Pulse identification method, pulse identification device, pulse identification storage medium, pulse identification equipment and blood cell analyzer | |
| CN111781168B (en) | Method, device, equipment and medium for adjusting red blood cell fragment recognition area | |
| CN118209472A (en) | Impedance channel hole blocking identification method, sample analyzer and related device | |
| CN115901580A (en) | Method, system and device for identifying blocked hole of particle channel, equipment and storage medium | |
| CN115201270A (en) | Blood detection method and blood analysis system | |
| CN115372207A (en) | Sample detection method, sample detection device and computer-readable storage medium | |
| JP2714629B2 (en) | Particle counting method and device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |