CN117100635A - Active preparation suitable for repairing cell tissues - Google Patents
Active preparation suitable for repairing cell tissues Download PDFInfo
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- CN117100635A CN117100635A CN202210526089.XA CN202210526089A CN117100635A CN 117100635 A CN117100635 A CN 117100635A CN 202210526089 A CN202210526089 A CN 202210526089A CN 117100635 A CN117100635 A CN 117100635A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses an active preparation suitable for repairing cell tissues, which relates to the technical field of tissue repair and comprises the following raw materials in parts by weight: the active preparation suitable for repairing cell tissues is prepared from 10-20 parts of chitosan, 10-15 parts of giant salamander small molecular peptide, 8-18 parts of collagen, 10-20 parts of whitening surfactant, 20-40 parts of deionized water, 2-13 parts of antioxidant and 3-11 parts of emulsifier, wherein the giant salamander is rich in abundant protein substances and amino acids, and has the characteristics of smaller molecular weight, easier absorption and utilization by human bodies, capability of well supplementing cell nutrition, repairing cell tissues, promoting skin repair, improving organism immunity and bacteriostasis pharmacological effects, and whitening after the giant salamander is subjected to enzymolysis and purification to obtain peptide.
Description
Technical Field
The invention relates to the technical field of tissue repair, in particular to an active preparation suitable for repairing cell tissues.
Background
Skin is an important organ of the human body and has the functions of controlling body temperature, preventing infection and body fluid loss, and immunity and sensing. Extensive damage to the skin may result from wounds, abrasions, skin ulcers, burns, and the like. Skin damage can easily cause bacterial infection and fluid loss, and cause various clinical complications.
The chitosan has the functions of relieving pain, stopping bleeding, promoting wound healing, reducing scars, inhibiting bacteria, and having good biocompatibility, biodegradability, good film forming property, anticoagulation, promoting wound healing, corrosion prevention, bacteria resistance and the like.
The giant salamander small molecular peptide can promote skin repair, improve organism immunity and antibacterial pharmacological action, collagen can participate in migration, differentiation and proliferation of cells, so that bones, tendons, cartilages and skin have certain mechanical strength, and the collagen has weak antigenicity and good biocompatibility, so that the giant salamander small molecular peptide is widely applied to the fields of burn, wound, cornea diseases, beauty, orthopedics, hard tissue repair, wound hemostasis and the like.
The existing active preparation for repairing the cell tissues has the problem of unsatisfactory repairing effect in promoting the skin to repair conveniently.
Disclosure of Invention
The present invention aims to provide an active agent suitable for repairing a tissue, which solves the problems set forth in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
an active preparation suitable for repairing cell tissues comprises the following raw materials in parts by weight: 10-20 parts of chitosan, 10-15 parts of giant salamander small molecule peptide, 8-18 parts of collagen, 10-20 parts of whitening surfactant, 20-40 parts of deionized water, 2-13 parts of antioxidant and 3-11 parts of emulsifier.
As a further scheme of the invention: the material comprises the following raw materials in parts by weight: 10 parts of chitosan, 15 parts of giant salamander small molecule peptide, 8 parts of collagen, 10 parts of whitening surfactant, 20 parts of deionized water, 2 parts of antioxidant and 11 parts of emulsifier.
As still further aspects of the invention: the material comprises the following raw materials in parts by weight: 20 parts of chitosan, 10 parts of giant salamander small molecule peptide, 18 parts of collagen, 20 parts of whitening surfactant, 40 parts of deionized water, 13 parts of antioxidant and 3 parts of emulsifier.
As still further aspects of the invention: the material comprises the following raw materials in parts by weight: 15 parts of chitosan, 12.5 parts of giant salamander small molecular peptide, 13 parts of collagen, 15 parts of whitening surfactant, 30 parts of deionized water, 7.5 parts of antioxidant and 7 parts of emulsifier.
As still further aspects of the invention: the material comprises the following raw materials in parts by weight: 11 parts of chitosan, 12 parts of giant salamander small molecule peptide, 9 parts of collagen, 11 parts of whitening surfactant, 21 parts of deionized water, 3 parts of antioxidant and 4 parts of emulsifier.
As still further aspects of the invention: the material comprises the following raw materials in parts by weight: 10-20 parts of chitosan, 14 parts of giant salamander small molecule peptide, 17 parts of collagen, 19 parts of whitening surfactant, 39 parts of deionized water, 12 parts of antioxidant and 10 parts of emulsifier.
As still further aspects of the invention: 12 parts of chitosan, 12 parts of giant salamander small molecule peptide, 10 parts of collagen, 12 parts of whitening surfactant, 22 parts of deionized water, 4 parts of antioxidant and 5 parts of emulsifier.
As still further aspects of the invention: 18 parts of chitosan, 13 parts of giant salamander small molecule peptide, 16 parts of collagen, 18 parts of whitening surfactant, 38 parts of deionized water, 11 parts of antioxidant and 9 parts of emulsifier.
As still further aspects of the invention: the whitening surfactant is mainly prepared from lauroyl amidopropyl betaine, hexadecyl betaine, banana extract and simethicone.
A preparation method of an active preparation suitable for repairing cell tissues comprises the following steps:
step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
step 4, slowly cooling the solution B, stirring the solution B at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, and stirring to prepare the active preparation suitable for repairing the cell tissues
Compared with the prior art, the invention has the beneficial effects that:
1. the active preparation suitable for repairing cell tissues is rich in rich protein substances and amino acids, has smaller molecular weight, is easier to be absorbed and utilized by human bodies after being subjected to enzymolysis and purification to obtain peptides, and can well supplement cell nutrition, thereby achieving the effects of repairing cell tissues, promoting skin repair, improving organism immunity and bacteriostasis pharmacological actions;
2. the preparation can enhance the activity of cells and reduce the deposition of melanin near the wound, so that the preparation has the characteristics of accelerating wound healing and whitening.
Detailed Description
The embodiment of the invention provides an active preparation suitable for repairing cell tissues, which comprises the following raw materials in parts by weight: 10-20 parts of chitosan, 10-15 parts of giant salamander small molecule peptide, 8-18 parts of collagen, 10-20 parts of whitening surfactant, 20-40 parts of deionized water, 2-13 parts of antioxidant and 3-11 parts of emulsifier.
Example 1
In this embodiment, an active agent suitable for repairing a tissue comprises the following raw materials in parts by weight: 10 parts of chitosan, 15 parts of giant salamander small molecule peptide, 8 parts of collagen, 10 parts of whitening surfactant, 20 parts of deionized water, 2 parts of antioxidant and 11 parts of emulsifier.
Step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B, stirring the solution B at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, and stirring to prepare the active preparation suitable for repairing the cell tissues.
Example 2
In this embodiment, an active agent suitable for repairing a tissue comprises the following raw materials in parts by weight: 10 parts of chitosan, 15 parts of giant salamander small molecule peptide, 8 parts of collagen, 10 parts of whitening surfactant, 20 parts of deionized water, 2 parts of antioxidant and 11 parts of emulsifier.
Step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B and stirring at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, stirring to prepare the active preparation suitable for repairing the cell tissues, smearing the prepared active preparation on the damaged skin surface of a volunteer, visually observing the damaged skin recovery state, using a multiparameter skin tester M150/canegari to test the melanin deposition value of the volunteer, and performing the test once a week for 4 weeks.
Example 3
In this embodiment, an active agent suitable for repairing a tissue comprises the following raw materials in parts by weight: 20 parts of chitosan, 10 parts of giant salamander small molecule peptide, 18 parts of collagen, 20 parts of whitening surfactant, 40 parts of deionized water, 13 parts of antioxidant and 3 parts of emulsifier.
Step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B and stirring at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, stirring to prepare the active preparation suitable for repairing the cell tissues, smearing the prepared active preparation on the damaged skin surface of a volunteer, visually observing the damaged skin recovery state, using a multiparameter skin tester M150/canegari to test the melanin deposition value of the volunteer, and performing the test once a week for 4 weeks.
Example 4
In this embodiment, an active agent suitable for repairing a tissue comprises the following raw materials in parts by weight: 15 parts of chitosan, 12.5 parts of giant salamander small molecular peptide, 13 parts of collagen, 15 parts of whitening surfactant, 30 parts of deionized water, 7.5 parts of antioxidant and 7 parts of emulsifier.
Step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B and stirring at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, stirring to prepare the active preparation suitable for repairing the cell tissues, smearing the prepared active preparation on the damaged skin surface of a volunteer, visually observing the damaged skin recovery state, using a multiparameter skin tester M150/canegari to test the melanin deposition value of the volunteer, and performing the test once a week for 4 weeks.
Example 5
In this embodiment, an active agent suitable for repairing a tissue comprises the following raw materials in parts by weight: 11 parts of chitosan, 12 parts of giant salamander small molecule peptide, 9 parts of collagen, 11 parts of whitening surfactant, 21 parts of deionized water, 3 parts of antioxidant and 4 parts of emulsifier.
Step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B and stirring at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, stirring to prepare the active preparation suitable for repairing the cell tissues, smearing the prepared active preparation on the damaged skin surface of a volunteer, visually observing the damaged skin recovery state, using a multiparameter skin tester M150/canegari to test the melanin deposition value of the volunteer, and performing the test once a week for 4 weeks.
Example 6
In this embodiment, an active agent suitable for repairing a tissue comprises the following raw materials in parts by weight: 10-20 parts of chitosan, 14 parts of giant salamander small molecule peptide, 17 parts of collagen, 19 parts of whitening surfactant, 39 parts of deionized water, 12 parts of antioxidant and 10 parts of emulsifier.
Step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B and stirring at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, stirring to prepare the active preparation suitable for repairing the cell tissues, smearing the prepared active preparation on the damaged skin surface of a volunteer, visually observing the damaged skin recovery state, using a multiparameter skin tester M150/canegari to test the melanin deposition value of the volunteer, and performing the test once a week for 4 weeks.
Example 7
In this embodiment, an active agent suitable for repairing a tissue comprises the following raw materials in parts by weight: 12 parts of chitosan, 12 parts of giant salamander small molecule peptide, 10 parts of collagen, 12 parts of whitening surfactant, 22 parts of deionized water, 4 parts of antioxidant and 5 parts of emulsifier.
Step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B and stirring at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, stirring to prepare the active preparation suitable for repairing the cell tissues, smearing the prepared active preparation on the damaged skin surface of a volunteer, visually observing the damaged skin recovery state, using a multiparameter skin tester M150/canegari to test the melanin deposition value of the volunteer, and performing the test once a week for 4 weeks.
Example 8
In this embodiment, an active agent suitable for repairing a tissue comprises the following raw materials in parts by weight: 18 parts of chitosan, 13 parts of giant salamander small molecule peptide, 16 parts of collagen, 18 parts of whitening surfactant, 38 parts of deionized water, 11 parts of antioxidant and 9 parts of emulsifier.
Step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B and stirring at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, stirring to prepare the active preparation suitable for repairing the cell tissues, smearing the prepared active preparation on the damaged skin surface of a volunteer, visually observing the damaged skin recovery state, using a multiparameter skin tester M150/canegari to test the melanin deposition value of the volunteer, and performing the test once a week for 4 weeks.
Comparing the observations in the above examples with volunteers not coated with the active agent, one can obtain: the active preparation suitable for repairing cell tissues is rich in rich protein substances and amino acids, has smaller molecular weight, is easier to be absorbed and utilized by human bodies after being subjected to enzymolysis and purification to obtain peptides, and can well supplement cell nutrition, thereby achieving the effects of repairing cell tissues, promoting skin repair, improving organism immunity and bacteriostasis pharmacological actions; the invention can enhance the activity of cells and reduce the deposition of melanin near the wound, so that the preparation has the characteristics of accelerating the wound healing and whitening.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (10)
1. An active preparation suitable for repairing a tissue, which is characterized by comprising the following raw materials in parts by weight: 10-20 parts of chitosan, 10-15 parts of giant salamander small molecule peptide, 8-18 parts of collagen, 10-20 parts of whitening surfactant, 20-40 parts of deionized water, 2-13 parts of antioxidant and 3-11 parts of emulsifier.
2. An active agent suitable for repairing a tissue according to claim 1, comprising the following raw materials in parts by weight: 10 parts of chitosan, 15 parts of giant salamander small molecule peptide, 8 parts of collagen, 10 parts of whitening surfactant, 20 parts of deionized water, 2 parts of antioxidant and 11 parts of emulsifier.
3. An active agent suitable for repairing a tissue according to claim 1, comprising the following raw materials in parts by weight: 20 parts of chitosan, 10 parts of giant salamander small molecule peptide, 18 parts of collagen, 20 parts of whitening surfactant, 40 parts of deionized water, 13 parts of antioxidant and 3 parts of emulsifier.
4. An active agent suitable for repairing a tissue according to claim 1, comprising the following raw materials in parts by weight: 15 parts of chitosan, 12.5 parts of giant salamander small molecular peptide, 13 parts of collagen, 15 parts of whitening surfactant, 30 parts of deionized water, 7.5 parts of antioxidant and 7 parts of emulsifier.
5. An active agent suitable for repairing a tissue according to claim 1, comprising the following raw materials in parts by weight: 11 parts of chitosan, 12 parts of giant salamander small molecule peptide, 9 parts of collagen, 11 parts of whitening surfactant, 21 parts of deionized water, 3 parts of antioxidant and 4 parts of emulsifier.
6. An active preparation suitable for repairing a tissue, which is characterized by comprising the following raw materials in parts by weight: 10-20 parts of chitosan, 14 parts of giant salamander small molecule peptide, 17 parts of collagen, 19 parts of whitening surfactant, 39 parts of deionized water, 12 parts of antioxidant and 10 parts of emulsifier.
7. An active preparation suitable for repairing a tissue, which is characterized by comprising the following raw materials in parts by weight: 12 parts of chitosan, 12 parts of giant salamander small molecule peptide, 10 parts of collagen, 12 parts of whitening surfactant, 22 parts of deionized water, 4 parts of antioxidant and 5 parts of emulsifier.
8. An active preparation suitable for repairing a tissue, which is characterized by comprising the following raw materials in parts by weight: 18 parts of chitosan, 13 parts of giant salamander small molecule peptide, 16 parts of collagen, 18 parts of whitening surfactant, 38 parts of deionized water, 11 parts of antioxidant and 9 parts of emulsifier.
9. An active agent suitable for repairing tissue according to claim 4, wherein said whitening surfactant is mainly made of lauramidopropyl betaine, cetyl betaine, banana extract and dimethicone.
10. An active agent for repairing a tissue according to any one of claims 1 to 9, wherein the method for preparing the active agent for repairing a tissue comprises the steps of:
step 1, weighing chitosan, giant salamander small molecular peptide, collagen, a whitening surfactant, an antioxidant and an emulsifier according to the weight parts, and mixing to obtain a mixture A;
step 2, heating deionized water, adding lauroyl amidopropyl betaine, controlling the temperature to be 50-55 ℃, stirring at 200r/min until lauroyl amidopropyl betaine is dissolved, controlling the temperature to be 50-55 ℃, and stirring at 100r/min, and adding hexadecyl betaine to obtain a solution B;
step 3, mixing the banana extract with simethicone, stirring at 40-45 ℃ for 200r/min, stirring for 3-5min, and cooling to obtain a solution C;
and 4, slowly cooling the solution B, stirring the solution B at 100r/min, adding the solutions A and C when the temperature of the solution B is reduced to 30 ℃, and stirring to prepare the active preparation suitable for repairing the cell tissues.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202210526089.XA CN117100635A (en) | 2022-05-16 | 2022-05-16 | Active preparation suitable for repairing cell tissues |
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| CN202210526089.XA CN117100635A (en) | 2022-05-16 | 2022-05-16 | Active preparation suitable for repairing cell tissues |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119157907A (en) * | 2024-11-21 | 2024-12-20 | 云南贺尔思细胞生物技术有限公司 | A stem cell composition for promoting skin cell repair and its preparation method and application |
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2022
- 2022-05-16 CN CN202210526089.XA patent/CN117100635A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119157907A (en) * | 2024-11-21 | 2024-12-20 | 云南贺尔思细胞生物技术有限公司 | A stem cell composition for promoting skin cell repair and its preparation method and application |
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