CN117025309A - Neutral low-foam medical complex enzyme cleaning agent and preparation method and application thereof - Google Patents

Abstract

The invention discloses a neutral low-foam medical complex enzyme cleaning agent, and a preparation method and application thereof. Relates to the technical field of cleaning agents. The complex enzyme cleaning agent comprises the following components: neutral proteases, neutral lipases, amylases, nucleases, enzyme reversible inhibitors and enzyme stabilizers. The main active substances of the enzyme cleaning agent are organic macromolecular active substances, so that corrosion to surgical instruments is reduced, and the service life of the surgical instruments is greatly prolonged. The enzyme stabilizer and the enzyme reversible inhibitor are added into the complex enzyme cleaning agent, so that the enzyme activity in the complex enzyme cleaning agent is effectively prolonged. The cleaning agent can quickly decompose dirt on the surface of the instrument into particles to separate, and the anti-deposition effect of the cleaning agent enables the immersed instrument to be easy to overflow, the pH value of the cleaning agent is close to neutral, and the cleaning agent is free from corrosiveness in manual scrubbing and avoids irritation to skin.

Description

A neutral and low-foaming medical compound enzyme cleaning agent and its preparation method and application

Technical field

The invention relates to the technical field of cleaning agents, and in particular to a neutral and low-foaming medical compound enzyme cleaning agent and its preparation method and application.

Background technique

Currently, existing cleaning agents for medical devices on the market generally have technical problems such as high pollution, weak decontamination ability, more foam produced during the cleaning process, incomplete cleaning, and water pollution after cleaning. Moreover, traditional cleaning agents are only simple Cleaning does not have the effect of sterilization. Conventional cleaning agents are difficult to completely decompose and remove biological residues or oil stains in a short period of time, and the cleaning effect is poor. Today, with the rapid development of biotechnology, enzyme catalytic technology has been widely used in all walks of life. Especially in the washing industry, the application proportion of enzyme preparations is also increasing year by year, mainly including protease, amylase, lipase, cellulose Enzymes and a series of hydrolytic enzymes. By adding biological enzymes to traditional cleaning agents, the amount of surfactant can be reduced, the cleaning effect can be improved, stains can be cleaned more specifically, and the cleaning temperature can be lower, which greatly saves cleaning energy consumption. However, since there are a large number of medical devices in hospitals, a large amount of cleaning agents need to be stored. After being stored for a long time, the cleaning agents are prone to decomposition and reduce their cleaning effect. Moreover, due to the corrosive nature of the cleaning agents, it is also easy to be damaged during the cleaning process. Causes damage to medical devices, thereby affecting the service life of medical devices.

Based on this, a new type of compound enzyme cleaning agent is urgently needed to solve the above problems.

Contents of the invention

The first technical problem to be solved by this invention is:

A composite enzyme cleaning agent is provided.

The second technical problem to be solved by this invention is:

A method for preparing the composite enzyme cleaning agent is provided.

The third technical problem to be solved by this invention is:

The invention also provides a cleaning agent for medical instruments.

In order to solve the first technical problem, the technical solution adopted by the present invention is:

A composite enzyme cleaning agent includes the following components:

neutral protease;

neutral lipase;

amylase;

nuclease;

Enzyme reversible inhibitors;

Enzyme stabilizer.

According to the embodiment of the present invention, one of the technical solutions has at least one of the following advantages or beneficial effects:

The present invention improves the stability of enzymes by adding components such as enzyme stabilizers. Compared with other technical inventions, the present invention has the following beneficial effects:

1) The main active substance of the enzyme cleaning agent of the present invention is an organic macromolecular active substance, which reduces the corrosion of surgical instruments and greatly extends the service life of surgical instruments.

2) The composite enzyme cleaning agent of the present invention adds an enzyme stabilizer and an enzyme reversible inhibitor, which effectively prolongs the enzyme activity in the composite enzyme cleaning agent.

3) The cleaning agent of the present invention can quickly decompose the dirt on the surface of the instrument into particles and separate them. Its anti-deposition effect makes the soaked instrument easy to pass through water. The pH value of the cleaning agent of the present invention is close to neutral, and manual scrubbing is no problem. Corrosive, avoid irritation to skin.

4) The cleaning agent of the present invention can effectively play a certain antibacterial effect. Since bacterial endotoxin is composed of protein in the center and lipopolysaccharide in its outer layer, the lipase in the biological enzyme cleaning agent can emulsify fat and increase the size of the bacteria. Its contact area with digestive enzymes can digest lipids and fatty acids and inactivate bacterial endotoxins. Amylase can quickly decompose various monosaccharides and polysaccharides in the outer layer of proteins. Under the combined action of the two, the outer layer can be The lipopolysaccharide is completely removed, and the internal proteins lose the protection of the outer membrane and are exposed to proteases. The proteins are eventually digested by proteolytic enzymes, thereby completely eliminating bacteria.

5) The present invention adds nuclease as one of the components of the complex enzyme, which directly acts on free sticky DNA and degrades it, thereby enhancing the cleaning effect of the cleaning agent.

According to an embodiment of the present invention, the composite enzyme cleaning agent includes the following components by weight:

3-11 parts of neutral protease;

3-5 parts of neutral lipase;

Neutral amylase 2-3;

2-3 parts of nuclease;

Enzyme reversible inhibitor 0.01-5 parts;

5-10 parts of enzyme stabilizer.

According to an embodiment of the present invention, the composite enzyme cleaning agent further includes the following components:

1-3 parts of corrosion and scale inhibitor;

5-10 parts of surfactant;

5-10 parts of fungicide;

5-15 parts of dispersant;

3-5 parts of defoaming agent.

According to one embodiment of the present invention, the enzyme reversible inhibitor includes at least one of EDTA-2Na salt, EDTA-4Na salt and protease inhibitor Cocktail. Enzyme reversible inhibitors have good chelation properties and can complex with metal ions in protease molecules, thereby reducing the activity of protease and reducing the decomposition of other active enzymes by protease. In the cleaning agent of the present invention, both an enzyme reversible inhibitor and a protease are added. In order to ensure the enzyme activity in the cleaning agent of the present invention and the cleaning performance of the cleaning agent, it is necessary to strictly select the enzyme reversible inhibitor and balance the enzyme reversibility. The dosage of inhibitors and proteases is to promote the balance and stability of the system.

According to an embodiment of the present invention, the enzyme stabilizer includes at least one of ethoxypolyethylenimine, boric acid, and calcium chloride. The enzyme stabilizer is preferably ethoxy polyethylene imine. Ethoxy polyethylene imine is added to the detergent as a stabilizer for protease. It can well improve the stability of protease and can also synergize with protease to remove the protease. The dirt performance is improved.

According to an embodiment of the present invention, the corrosion and scale inhibitor includes at least one of polyaspartic acid sodium salt, sodium gluconate and benzotriazole. Corrosion and scale inhibitors have good chelating properties and can chelate a variety of metal ions in hard water to form soft scale, which is beneficial to improving the effect of enzymes and surfactants during the washing process. The invention adds a corrosion and scale inhibitor to slow down the damage of the cleaning agent to the medical equipment and improve the service life of the medical equipment.

According to an embodiment of the present invention, the bactericidal agent includes at least one of benzalkonium chloride, benzalkonium bromide and benzethonium chloride. The invention adds a certain amount of bactericide so that the cleaning agent has certain bactericidal performance. The preferred bactericide is benzalkonium chloride. As a quaternary ammonium salt, benzalkonium chloride has excellent bactericidal properties and can kill most common bacteria. It is also environmentally friendly and can kill bacteria on the surface of medical devices, improving improve the safety of medical devices.

According to an embodiment of the present invention, the dispersant includes at least one of acrylic acid homopolymer or copolymer and maleic acid homopolymer or copolymer, and its molecular weight is 3,000-70,000. Preferably, the dispersant is polyacrylic acid homopolymer. Polyacrylic acid homopolymer can form stable compounds with metal ions, calcium, magnesium, etc., has excellent decomposition effect on calcium carbonate and calcium hydroxide in water, and can improve the cleaning agent system. The dispersion property also prevents dirt from redepositing on the metal surface.

According to an embodiment of the present invention, the defoaming agent includes at least one of polyether polyol and polyether modified silicone. The cleaning agent of the present invention adds a defoaming agent, so that the foam is low during the cleaning process, the liquid is transparent and convenient for cleaning operation, and the decontamination ability of the cleaning agent is also enhanced. Preferably, the defoaming agent is polyether-modified silicone. In the molecule of the silicon ether copolymer, the siloxane segment is a lipophilic group and the polyether segment is a hydrophilic group, so it can be used for both oily and water-based products. This kind of foaming system can quickly eliminate foam and exert its continuous foam suppression function for a long time.

In order to solve the second technical problem, the technical solution adopted by the present invention is:

A method for preparing the composite enzyme cleaning agent, including the following steps:

Mix neutral protease, neutral lipase, amylase, nuclease, enzyme reversible inhibitor and enzyme stabilizer in a solvent to obtain the composite enzyme cleaning agent.

According to an embodiment of the present invention, a method for preparing the composite enzyme cleaning agent includes the following steps:

S1. Add surfactant, dispersant, corrosion and scale inhibitor, defoaming agent, and enzyme stabilizer to deionized water, stir evenly, and prepare the first mixed liquid;

S2. Add neutral protease to the first mixed liquid and stir for 10-30 minutes to prepare the second mixed liquid;

S3. Add neutral lipase, amylase and nuclease in order to the second mixed solution, and stir for 10-30 minutes to obtain the composite enzyme cleaning agent.

In another aspect of the present invention, a cleaning agent for medical devices is also provided. Including the composite enzyme cleaning agent as described in the first embodiment above. Since this application adopts all the technical solutions of the above-mentioned composite enzyme cleaning agent, it has at least all the beneficial effects brought by the technical solutions of the above embodiments.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be apparent from the description, or may be learned by practice of the invention.

Detailed ways

In the description of the present invention, if the first, second, etc. are described, they are only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of indicated technical features or implying Indicate the sequence relationship of the indicated technical features.

In the description of the present invention, it should be understood that the orientation descriptions, such as the orientations or positional relationships indicated by up and down, etc. are based on the orientations or positional relationships shown in the embodiments, and are only for the convenience of describing the present invention and simplifying the description. , rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be construed as a limitation of the present invention.

The words "preferably", "more preferably", etc. in the present invention refer to embodiments of the present invention that can provide certain beneficial effects under certain circumstances. However, other embodiments may also be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not available, nor is it intended to exclude other embodiments from the scope of the invention.

When a numerical range is disclosed herein, such range is deemed to be continuous and includes the minimum and maximum values of the range, and every value between such minimum and maximum values. Further, when a range refers to an integer, every integer between the minimum value and the maximum value of the range is included. Additionally, when multiple ranges are provided to describe a feature or characteristic, the ranges can be combined. In other words, unless otherwise indicated, all ranges disclosed herein are to be understood to include any and all subranges subsumed therein.

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of the present invention.

The reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in this technical field unless otherwise specified.

Example 1

A composite enzyme cleaning agent includes the following components by weight:

4 parts of neutral protease;

4 parts of neutral lipase;

3 parts neutral amylase;

2 copies of nuclease;

3 parts of enzyme reversible inhibitor;

10 parts of enzyme stabilizer;

2 parts of corrosion and scale inhibitor;

5 parts surfactant;

5 parts of fungicide;

10 parts of dispersant;

4 parts of defoaming agent.

Among them, the enzyme reversible inhibitor uses EDTA-2Na salt;

Among them, ethoxy polyethylenimine is used as the enzyme stabilizer;

Among them, sodium gluconate is used as the corrosion and scale inhibitor;

Among them, alkyl glycosides are used as surfactants;

Among them, benzalkonium chloride is used as the fungicide;

Among them, the dispersant is acrylic acid homopolymer;

Among them, polyether modified silicone is used as the defoaming agent.

Preparing the above-mentioned composite enzyme cleaning agent includes the following steps:

S1 Mix enzyme stabilizer, corrosion and scale inhibitor, surfactant, bactericide, dispersant and defoaming agent in deionized water, stir evenly to prepare the first mixed liquid;

S2 sequentially add neutral protease and enzyme reversible inhibitor to the first mixed liquid, stir for 20 minutes, and prepare a second mixed liquid;

S3 Add neutral lipase, amylase and nuclease to the second mixed solution in sequence, and stir for 20 minutes to obtain a composite enzyme cleaning agent.

Example 2

A composite enzyme cleaning agent includes the following components by weight:

3 parts of neutral protease;

3 parts of neutral lipase;

2 parts neutral amylase;

2 copies of nuclease;

5 parts of enzyme reversible inhibitor;

5 parts of enzyme stabilizer;

1 part of corrosion and scale inhibitor;

5 parts surfactant;

7 parts of fungicide;

5 parts of dispersant;

3 parts of defoaming agent.

Among them, the enzyme reversible inhibitor uses EDTA-2Na salt;

Among them, ethoxy polyethylenimine is used as the enzyme stabilizer;

Among them, sodium gluconate is used as the corrosion and scale inhibitor;

Among them, alkyl glycosides are used as surfactants;

Among them, benzethonium chloride is selected as the fungicide;

Among them, the dispersant is acrylic acid homopolymer;

Among them, polyether modified silicone is used as the defoaming agent.

Preparing the above-mentioned composite enzyme cleaning agent includes the following steps:

S1 Mix enzyme stabilizer, corrosion and scale inhibitor, surfactant, bactericide, dispersant and defoaming agent in deionized water, stir evenly to prepare the first mixed liquid;

S2 sequentially add neutral protease and enzyme reversible inhibitor to the first mixed liquid, stir for 20 minutes, and prepare a second mixed liquid;

S3 Add neutral lipase, amylase and nuclease to the second mixed solution in sequence, and stir for 20 minutes to obtain a composite enzyme cleaning agent.

Example 3

A composite enzyme cleaning agent includes the following components by weight:

6 parts of neutral protease;

5 parts of neutral lipase;

3 parts neutral amylase;

3 copies of nuclease;

Enzyme reversible inhibitor 0.01 parts;

10 parts of enzyme stabilizer;

3 parts of corrosion and scale inhibitor;

10 parts of surfactant;

5 parts of fungicide;

10 parts of dispersant;

5 parts of defoaming agent.

Among them, the enzyme reversible inhibitor uses Cocktail protease inhibitor;

Among them, ethoxy polyethylenimine is used as the enzyme stabilizer;

Among them, sodium gluconate is used as the corrosion and scale inhibitor;

Among them, alkyl glycosides are used as surfactants;

Among them, benzalkonium bromide is selected as the fungicide;

Among them, the dispersant is acrylic acid homopolymer;

Among them, polyether modified silicone is used as the defoaming agent.

Preparing the above-mentioned composite enzyme cleaning agent includes the following steps:

S1 Mix enzyme stabilizer, corrosion and scale inhibitor, surfactant, bactericide, dispersant and defoaming agent in deionized water, stir evenly to prepare the first mixed liquid;

S2 sequentially add neutral protease and enzyme reversible inhibitor to the first mixed liquid, stir for 20 minutes, and prepare a second mixed liquid;

S3 Add neutral lipase, amylase and nuclease to the second mixed solution in sequence, and stir for 20 minutes to obtain a composite enzyme cleaning agent.

Example 4

A composite enzyme cleaning agent includes the following components by weight:

4 parts of neutral protease;

4 parts of neutral lipase;

3 parts neutral amylase;

2 copies of nuclease;

1 part of enzyme reversible inhibitor;

10 parts of enzyme stabilizer;

2 parts of corrosion and scale inhibitor;

5 parts surfactant;

10 parts of fungicide;

10 parts of dispersant;

4 parts of defoaming agent.

Among them, the enzyme reversible inhibitor uses EDTA-2Na salt;

Among them, ethoxy polyethylenimine is used as the enzyme stabilizer;

Among them, sodium gluconate is used as the corrosion and scale inhibitor;

Among them, alkyl glycosides are used as surfactants;

Among them, benzalkonium chloride is used as the fungicide;

Among them, the dispersant is acrylic acid homopolymer;

Among them, polyether modified silicone is used as the defoaming agent.

Preparing the above-mentioned composite enzyme cleaning agent includes the following steps:

S1 Mix enzyme stabilizer, corrosion and scale inhibitor, surfactant, bactericide, dispersant and defoaming agent in deionized water, stir evenly to prepare the first mixed liquid;

S2 sequentially add neutral protease and enzyme reversible inhibitor to the first mixed liquid, stir for 20 minutes, and prepare a second mixed liquid;

S3 Add neutral lipase, amylase and nuclease to the second mixed solution in sequence, and stir for 20 minutes to obtain a composite enzyme cleaning agent.

Example 5

A composite enzyme cleaning agent includes the following components by weight:

4 parts of neutral protease;

4 parts of neutral lipase;

3 parts neutral amylase;

2 copies of nuclease;

5 parts of enzyme reversible inhibitor;

10 parts of enzyme stabilizer;

2 parts of corrosion and scale inhibitor;

5 parts surfactant;

10 parts of fungicide;

10 parts of dispersant;

4 parts of defoaming agent.

Among them, the enzyme reversible inhibitor uses EDTA-2Na salt;

Among them, ethoxy polyethylenimine is used as the enzyme stabilizer;

Among them, sodium gluconate is used as the corrosion and scale inhibitor;

Among them, alkyl glycosides are used as surfactants;

Among them, benzalkonium chloride is used as the fungicide;

Among them, the dispersant is acrylic acid homopolymer;

Among them, polyether modified silicone is used as the defoaming agent.

Preparing the above-mentioned composite enzyme cleaning agent includes the following steps:

S1 Mix enzyme stabilizer, corrosion and scale inhibitor, surfactant, bactericide, dispersant and defoaming agent in deionized water, stir evenly to prepare the first mixed liquid;

S2 sequentially add neutral protease and enzyme reversible inhibitor to the first mixed liquid, stir for 20 minutes, and prepare a second mixed liquid;

S3 Add neutral lipase, amylase and nuclease to the second mixed solution in sequence, and stir for 20 minutes to obtain a composite enzyme cleaning agent.

Example 6

A composite enzyme cleaning agent includes the following components by weight:

4 parts of neutral protease;

4 parts of neutral lipase;

3 parts neutral amylase;

2 copies of nuclease;

3 parts of enzyme reversible inhibitor;

10 parts of enzyme stabilizer;

2 parts of corrosion and scale inhibitor;

5 parts surfactant;

5 parts of fungicide;

10 parts of dispersant;

4 parts of defoaming agent.

Among them, the enzyme reversible inhibitor uses EDTA-4Na salt;

Among them, ethoxy polyethylenimine is used as the enzyme stabilizer;

Among them, sodium gluconate is used as the corrosion and scale inhibitor;

Among them, alkyl glycosides are used as surfactants;

Among them, benzalkonium chloride is used as the fungicide;

Among them, the dispersant is acrylic acid homopolymer;

Among them, polyether modified silicone is used as the defoaming agent.

Preparing the above-mentioned composite enzyme cleaning agent includes the following steps:

S1 Mix enzyme stabilizer, corrosion and scale inhibitor, surfactant, bactericide, dispersant and defoaming agent in deionized water, stir evenly to prepare the first mixed liquid;

S2 sequentially add neutral protease and enzyme reversible inhibitor to the first mixed liquid, stir for 20 minutes, and prepare a second mixed liquid;

S3 Add neutral lipase, amylase and nuclease to the second mixed solution in sequence, and stir for 20 minutes to obtain a composite enzyme cleaning agent.

Example 7

A composite enzyme cleaning agent includes the following components by weight:

4 parts of neutral protease;

4 parts of neutral lipase;

3 parts neutral amylase;

2 copies of nuclease;

3 parts of enzyme reversible inhibitor;

10 parts of enzyme stabilizer;

2 parts of corrosion and scale inhibitor;

5 parts surfactant;

5 parts of fungicide;

10 parts of dispersant;

4 parts of defoaming agent.

Among them, the enzyme reversible inhibitor uses EDTA-2Na salt;

Among them, the enzyme stabilizer is boric acid and calcium chloride with a weight ratio of 1:1;

Among them, sodium gluconate is used as the corrosion and scale inhibitor;

Among them, alkyl glycosides are used as surfactants;

Among them, benzalkonium chloride is used as the fungicide;

Among them, the dispersant is acrylic acid homopolymer;

Among them, polyether modified silicone is used as the defoaming agent.

Preparing the above-mentioned composite enzyme cleaning agent includes the following steps:

S1 Mix enzyme stabilizer, corrosion and scale inhibitor, surfactant, bactericide, dispersant and defoaming agent in deionized water, stir evenly to prepare the first mixed liquid;

S2 sequentially add neutral protease and enzyme reversible inhibitor to the first mixed liquid, stir for 20 minutes, and prepare a second mixed liquid;

S3 Add neutral lipase, amylase and nuclease to the second mixed solution in sequence, and stir for 20 minutes to obtain a composite enzyme cleaning agent.

Example 8

A composite enzyme cleaning agent includes the following components by weight:

4 parts of neutral protease;

4 parts of neutral lipase;

3 parts neutral amylase;

2 copies of nuclease;

3 parts of enzyme reversible inhibitor;

10 parts of enzyme stabilizer;

2 parts of corrosion and scale inhibitor;

5 parts surfactant;

5 parts of fungicide;

10 parts of dispersant;

4 parts of defoaming agent.

Among them, the enzyme reversible inhibitor uses EDTA-2Na salt;

Among them, ethoxy polyethylenimine is used as the enzyme stabilizer;

Among them, the corrosion and scale inhibitors are polyaspartic acid sodium salt and benzotriazole with a weight ratio of 2:1;

Among them, alkyl glycosides are used as surfactants;

Among them, benzalkonium chloride is used as the fungicide;

Among them, the dispersant is acrylic acid homopolymer;

Among them, polyether modified silicone is used as the defoaming agent.

Preparing the above-mentioned composite enzyme cleaning agent includes the following steps:

S1 Mix enzyme stabilizer, corrosion and scale inhibitor, surfactant, bactericide, dispersant and defoaming agent in deionized water, stir evenly to prepare the first mixed liquid;

S2 sequentially add neutral protease and enzyme reversible inhibitor to the first mixed liquid, stir for 20 minutes, and prepare a second mixed liquid;

S3 Add neutral lipase, amylase and nuclease to the second mixed solution in sequence, and stir for 20 minutes to obtain a composite enzyme cleaning agent.

Performance Testing:

The composite enzyme cleaning agent prepared in Examples 1-5 was subjected to a cleaning test according to the cleaning effect requirement standard used in 7.6 of T_WSJD002-2019. The test results are shown in Table 1 below.

Table 1

As can be seen from Table 1 above, each test result shows that the composite enzyme cleaning agents prepared in Examples 1-5 all meet the cleaning effect requirements, indicating that the composite enzyme cleaning agents prepared by the present invention have good decontamination performance and sterilization performance.

Among them, the test results in Example 1, Example 4, and Example 5 show that adding too much or too little EDTA-2Na will lead to a decrease in the cleaning effect of the cleaning agent. Therefore, if EDTA-2Na needs to be added to the reaction system to stabilize and enhance enzyme activity, it needs to be at an appropriate ratio of EDTA-2Na to neutral protease so that it can form a relatively balanced solution with neutral protease. state. This can improve the application effect of EDTA-2Na and better protect enzyme activity.

The composite enzyme cleaning agent prepared in Examples 1-5 was subjected to a foam test according to the method in 11 of GB/T13173-2008. The foam test test standard: the initial foam height does not exceed 15 mm under 25°C conditions; under 40°C conditions The initial foam height does not exceed 10mm, and the test results are shown in Table 2 below.

Table 2

It can be seen from Table 2 above that all tests are in compliance with the foam test test standards. As the defoamer content increases, the initial foam height at 25°C and 40°C has a downward trend, indicating that the addition of defoamer can play a significant role. It inhibits foam formation. The high-efficiency, low-foam, bacteriostatic, multi-enzyme cleaning agent of the present invention contains a defoaming agent, has the advantage of less foam, and improves the efficiency of cleaning medical instruments.

The results of Examples 6 to 8 are similar to those of Example 1, and are not shown one by one to avoid redundancy.

In view of the problems existing in enzyme cleaning agents, the present invention has successfully developed a high-efficiency neutral and low-foaming composite enzyme cleaning agent by improving the cleaning agent formula. By adding enzyme stabilizers and enzyme reversible inhibitors, the stability of the enzymes is improved. And a bactericide is added to it to improve the bactericidal performance of the cleaning agent. In addition, defoaming agents are added to reduce the deterioration of cleaning effect caused by the generation of foam. At the same time, corrosion and scale inhibitors are added to slow down the damage of cleaning agents to medical devices and extend the service life of medical devices.

The above are only examples of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent transformations made using the contents of the description of the present invention, or directly or indirectly applied in related technical fields, are similarly included in the patent protection of the present invention. within the range.

Claims (10)

1. A composite enzyme cleaning agent, characterized in that: it includes the following components: neutral protease; neutral lipase; amylase; nuclease; Enzyme reversible inhibitors; Enzyme stabilizer. 2. A composite enzyme cleaning agent according to claim 1, characterized in that: the composite enzyme cleaning agent includes the following components by weight: 3-11 parts of neutral protease; 3-5 parts of neutral lipase; 2-3 parts of neutral amylase; 2-3 parts of nuclease; Enzyme reversible inhibitor 0.01-5 parts; 5-10 parts of enzyme stabilizer. 3. A composite enzyme cleaning agent according to claim 1, characterized in that: the composite enzyme cleaning agent further includes the following components: 1-3 parts of corrosion and scale inhibitor; 5-10 parts of surfactant; 5-10 parts of fungicide; 5-15 parts of dispersant; 3-5 parts of defoaming agent. 4. A composite enzyme cleaning agent according to claim 1, characterized in that: the enzyme reversible inhibitor includes at least one of EDTA-2Na salt, EDTA-4Na salt and protease inhibitor Cocktail. 5. A composite enzyme cleaning agent according to claim 1, characterized in that the enzyme stabilizer includes at least one of ethoxy polyethylenimine, boric acid and calcium chloride. 6. A composite enzyme cleaning agent according to claim 3, characterized in that: the corrosion and scale inhibitor includes at least one of polyaspartic acid sodium salt, sodium gluconate and benzotriazole. . 7. A composite enzyme cleaning agent according to claim 3, characterized in that the bactericide includes at least one of benzalkonium chloride, benzalkonium bromide and benzethonium chloride. 8. A composite enzyme cleaning agent according to claim 3, characterized in that the defoaming agent includes at least one of polyether polyol and polyether modified silicone. 9. A method for preparing the complex enzyme cleaning agent according to any one of claims 1 to 8, characterized in that: it includes the following steps: Mix neutral protease, neutral lipase, amylase, nuclease, enzyme reversible inhibitor and enzyme stabilizer in a solvent to obtain the composite enzyme cleaning agent. 10. A cleaning agent for medical devices, characterized in that it includes a composite enzyme cleaning agent according to any one of claims 1 to 8.