CN116983231A - Biological sun protection and repair barrier additive composition, preparation method and application thereof, and sun protection value testing method - Google Patents

Abstract

The invention provides a preparation method of a biological sunscreen and barrier repair additive composition and its application in sunscreen products; the invention can achieve a sunscreen effect relatively quickly, not only absorbing ultraviolet rays, but also inhibiting tyrosine The activity of acidase can provide sun protection from a deep level; the composition provided by the invention is not easy to cause allergies and can be added to an existing sun protection product or used independently. It can also be used in sun protection products for pregnant women and children. It feels refreshing and non-greasy; active ingredients extracted from two microalgae, Dunaliella salina and Haematococcus sanguineus, are rich in several carotenoids, which strengthen the protection of the skin: resist ultraviolet radiation, prevent sunburn, and fight inflammation. Eliminates redness and soothes skin.

Description

A biological sunscreen and barrier repair additive composition, preparation method, application and Test method for sun protection value

Technical field

The present invention relates to the technical field of daily chemicals, and in particular to a biological sunscreen and barrier repair additive composition, preparation method and application.

Background technique

Currently, there are three types of sunscreens on the market. One type is physical sunscreens, such as zinc oxide, titanium dioxide, etc., which mainly use their own physical properties to absorb and reflect ultraviolet rays. They also mainly retain It performs sun protection on the skin surface, and because the microstructure of the skin is a ravine-like structure, physical sunscreen agents can only reach a maximum sun protection index of spf20. In order to improve the sun protection performance of inorganic substances, consideration will be given to reducing the particle size of inorganic substances, or even Nanoscale. When it is at the nanometer level, there is the possibility of being absorbed by the skin, and there is a potential risk of allergy, and there is a risk of inhalation for children, so it is banned from children's products; the second category: chemical sunscreens, which are also often used A type of sunscreen. Although chemical sunscreens have excellent and fast sunscreen properties, they will be absorbed by the skin and even enter the blood. They will have a certain degree of toxicity and cause certain harm to the human body. Some people are very susceptible to allergies to chemical sunscreens. Symptoms; the third category is biological sunscreen, which is also the latest popular type of sunscreen in recent years. The biological sunscreen itself 1. has a certain ability to absorb ultraviolet rays. 2. Because ultraviolet radiation is an oxidative stress process that causes a series of tissue damage by producing oxygen free radicals. The substances contained in biological sunscreens can remove or reduce Oxygen reactive group intermediates, thereby blocking or slowing down tissue damage or promoting post-sun repair, playing an indirect sun protection role.

Biotype sunscreen is a type of sunscreen that is not prone to allergies. However, due to its mechanism of action and its inability to be used independently as a sunscreen, it is not widely used yet.

Contents of the invention

The purpose of the present invention is to provide a fast, long-lasting, and efficient sunscreen composition to solve the technical problem of the contradiction between sunscreen effect and usage amount existing in the prior art.

In order to solve the above technical problems, the present invention provides a biological sunscreen and barrier repair additive composition, which composition includes Glycine maxima seed oil, Dunaliella salina extract, and Haematococcus pluvialis extract. , Porphyra umbilicus extract; and the ratio of the Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract is: (10-30): (20-70): (0.1-1 ).

Preferably, the extraction method of the hairless water husk seed oil is as follows:

(1) Pre-treatment: collect the hairless water husk seeds, place them in an oven for drying, grind the dried hairless water husk seeds to obtain the hairless water husk seeds powder;

(2) Extraction: Place the glabrous water bark seed powder in an extraction bottle, add a mixed solvent according to a material-to-liquid ratio of 1:10, and extract at an ultrasonic power of 200W, an ultrasonic time of 30 minutes, and an ultrasonic temperature of 60°C. After completion, filter to obtain the filtrate and filter residue respectively;

(3) Mix the filter residue and culture medium together for culture. The culture conditions are to culture at 27±1°C for 7 days. Mix the obtained culture liquid with the filtrate in step (2). The strain is Bacillus subtilis. Bacillus; inoculum amount is 6%;

(4) Add an equal volume of deionized water to the mixed solution obtained in step (3) and let it stand. Take the upper liquid and then rotary evaporate it until it becomes a paste.

Preferably, the temperature in the oven is 80°C; and the mixed solvent is a mixed solvent of ethanol and n-propanol in a volume ratio of 2:3.

Preferably, the ratio of Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract is: (15-30): (25-70): (0.5-1).

Preferably, the ratio of Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract is: (20-30): (40-70): (0.5-1).

Preferably, the composition further includes milk thistle seed oil and Nannochloropsis ocularis extract.

Preferably, the composition is added to daily chemicals at a concentration of 0.1-5 wt% for use.

Another aspect of the present invention provides a method for preparing the biological sunscreen and barrier repair additive composition according to claim 6, which method includes:

S01 Dissolve the extract of Aquaphora glauca seed oil, Silybum marianum seed oil, and Nannochloropsis oculata extract in ethanol to obtain mixture 1;

S02 mix the remaining raw materials and the lower liquid from step (2) to obtain mixture 2;

S03 can be prepared by applying mixture 1 to the inner wall of the container, drying it, adding mixture 2, and then rotating and shaking at 35°C.

Another aspect of the present invention provides that the biological sunscreen and barrier repair additive composition can be used in the field of sunscreen.

Another aspect of the present invention provides that the biological sunscreen and barrier repair additive composition can be used in the field of skin barrier repair.

On the other hand, the present invention provides a testing method for testing sun protection value. The steps are as follows: apply the composition on a test position, and simultaneously perform gradient ultraviolet irradiation on different test positions of the same subject, and perform sun protection after irradiation. Numerical calculations.

Adopting the above technical solution, the present invention has the following beneficial effects:

1. The present invention can achieve a relatively good sun protection effect, not only absorbing ultraviolet rays, but also inhibiting the activity of tyrosinase, providing sun protection from a deep level;

2. The composition provided by the present invention is not prone to allergies and can be added to an existing sunscreen product or used independently. It can also be used in sunscreen products for pregnant women and children, with a refreshing and non-greasy skin feel;

3. Active ingredients extracted from two microalgae, Dunaliella salina and Haematococcus haemogenum, are rich in several carotenoids, which strengthen the protection of the skin: resist ultraviolet radiation, target sunburn, anti-inflammation, eliminate redness and swelling, Soothes skin. Through its own anti-inflammatory effect, it promotes capillary contraction (a powerful capillary constrictor) to delay the appearance of long-lasting erythema on the skin, thereby increasing the SPF value.

Description of drawings

In order to more clearly explain the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings that need to be used in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description These are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without exerting creative efforts.

Figure 1 is an SPF test chart (test comparison chart between Example 2 and Example 6).

Detailed ways

The technical solution of the present invention will be clearly and completely described below. Obviously, the described embodiments are some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

The present invention provides a biological sunscreen and barrier repair additive composition, which includes Porphyra lucidum seed oil, Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract; And the ratio of the Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract is:

(10-30):(20-70):(0.1-1).

PONGAMIA GLABRA Seed Oil: PONGAMIA GLABRA Seed Oil. In the present invention, the extraction method of the glabrous water bark seed oil is as follows:

(1) Pre-treatment: collect the hairless water husk seeds, place them in an oven for drying, grind the dried hairless water husk seeds to obtain hairless water husk seeds powder; preheat the oven , and the temperature is 80℃;

(2) Extraction: Place the glabrous water bark seed powder in an extraction bottle, add a mixed solvent according to a material-to-liquid ratio of 1:10, and extract at an ultrasonic power of 200W, an ultrasonic time of 30 minutes, and an ultrasonic temperature of 60°C. After completion, filter to obtain the filtrate and filter residue respectively; wherein, the mixed solvent is a mixed solvent of ethanol and n-propanol with a volume ratio of 2:3;

(3) Mix the filter residue and culture medium together for culture. The culture conditions are to culture at 27±1°C for 7 days. Mix the obtained culture liquid with the filtrate in step (2). The strain is Bacillus subtilis. Bacillus; inoculum amount is 6%;

The Bacillus subtilis was purchased from the China General Microbial Culture Collection and Management Center, and the preservation number is: 1.1498; the inoculum amount of the filter residue is 20%.

The components of the culture medium are: glucose 10g/L, inositol 0.01g/L, riboflavin 3.5g/L, vitamin B124.2mg/L, (NH ₄ ) ₂ SO ₄ 1g/L, MgSO ₄ ·7H ₂ O 0.2g/L, NaCl 0.3/L, K ₂ HPO ₄ 1g/L, the balance is water; natural pH.

(4) Add an equal volume of deionized water to the mixed solution obtained in step (3) and let it stand. Take the upper liquid and then rotary evaporate it until it becomes a paste.

Dunaliella salina extract: DUNALIELLA SALINA EXTRACT. The Dunaliella salina extract described in the present invention was purchased from Shaanxi Haibo Biotechnology Co., Ltd.

Haematococcus pluvialis extract: The Haematococcus pluvialis extract described in the present invention was purchased from Xi'an Muyuan Biotechnology Co., Ltd.

PORPHYRA UMBILICALIS extract: PORPHYRA UMBILICALIS extract was purchased from Kangdu Pharmaceutical Co., Ltd.

Preferably, the ratio of Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract is: (15-30): (25-70): (0.5-1).

More preferably, the ratio of Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract is: (20-30): (40-70): (0.5-1).

Further preferably, the composition further includes milk thistle seed oil and Nannochloropsis ocularis extract.

In the present invention, the milk thistle seed oil is purchased from Shanxi Wenyun Biotechnology Co., Ltd.; the Nannochloropsis oculata extract, that is, NANNOCHLOROPSIS OCULATA extract is purchased from Wuhan Jiuzhouyuan Biotechnology Co., Ltd.

In the present invention, the biological sunscreen and barrier repair additive composition is added to daily chemicals at a concentration of 0.1-5wt% for use.

Another aspect of the present invention provides a method for the biological sunscreen and barrier repair additive composition, which method includes:

S01 Dissolve the extract of Aquaphora glauca seed oil, Silybum marianum seed oil, and Nannochloropsis oculata extract in ethanol to obtain mixture 1;

S02 mix the remaining raw materials and the lower liquid from step (2) to obtain mixture 2;

S03 can be prepared by applying mixture 1 to the inner wall of the container, drying it, adding mixture 2, and then rotating and shaking at 35°C.

Another aspect of the present invention provides that the biological sunscreen and barrier repair additive composition can be used in the field of sunscreen.

Another aspect of the present invention provides that the biological sunscreen and barrier repair additive composition can be used in the field of skin barrier repair.

The inventor of the present application found during research that although the combination of Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract has certain sun protection properties, it was found that the three can only be used in a certain proportion. The possible reason for exerting sunscreen properties according to experiments is that Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract can all inhibit tyrosinase. However, when the ratio of the three exceeds a certain Within the range, there will be competitive inhibition among the three, so the sun protection effect will be reduced. The inventor can solve the above problem after adding the hairless water scallop seed oil to the composition. The possible reason is that the parent ring of the sunscreen component in the hairless water husk seed oil is benzopyran, and the above-mentioned containing Polyhydroxyl groups create a certain degree of steric hindrance, which can balance the above three components and at the same time expand the proportion range between the three, greatly reducing costs and the possibility of allergies.

The present invention will be further explained below in conjunction with specific embodiments.

Example 1

This embodiment provides a biological sunscreen and barrier-repairing additive composition, which includes Porphyra lucidum seed oil, Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract. , milk thistle seed oil, Nannochloropsis ocularis extract; and the mass ratio is 3:23:55:0.7:8:0.1.

Wherein, the extraction method of the glabrous water bark seed oil is as follows:

(1) Pre-treatment: collect the hairless water husk seeds, place them in an oven for drying, grind the dried hairless water husk seeds to obtain hairless water husk seeds powder; preheat the oven , and the temperature is 80℃;

(2) Extraction: Place the glabrous water bark seed powder in an extraction bottle, add a mixed solvent according to a material-to-liquid ratio of 1:10, and extract at an ultrasonic power of 200W, an ultrasonic time of 30 minutes, and an ultrasonic temperature of 60°C. After completion, filter to obtain the filtrate and filter residue respectively; wherein, the mixed solvent is a mixed solvent of ethanol and n-propanol with a volume ratio of 2:3;

(3) Mix the filter residue and culture medium together for culture. The culture conditions are to culture at 27±1°C for 7 days. Mix the obtained culture liquid with the filtrate in step (2). The strain is Bacillus subtilis. Bacillus; inoculum amount is 6%;

The Bacillus subtilis was purchased from the China General Microbial Culture Collection and Management Center, and the preservation number is: 1.1498; the inoculum amount of the filter residue is 20%.

The components of the culture medium are: glucose 10g/L, inositol 0.01g/L, riboflavin 3.5g/L, vitamin B124.2mg/L, (NH ₄ ) ₂ SO ₄ 1g/L, MgSO ₄ ·7H ₂ O 0.2g/L, NaCl 0.3/L, K ₂ HPO ₄ 1g/L, the balance is water; natural pH.

(4) Add an equal volume of deionized water to the mixed solution obtained in step (3) and let it stand. Take the upper liquid and then rotary evaporate it until it becomes a paste.

The Dunaliella salina extract was purchased from Shaanxi Haibo Biotechnology Co., Ltd.

The Haematococcus pluvialis extract was purchased from Xi'an Muyuan Biotechnology Co., Ltd.

PORPHYRA UMBILICALIS extract was purchased from Kangdu Pharmaceutical Co., Ltd.

The milk thistle seed oil was purchased from Shanxi Wenyun Biotechnology Co., Ltd.

NANNOCHLOROPSISOCULATA extract was purchased from Wuhan Jiuzhouyuan Biotechnology Co., Ltd.

Another aspect of this embodiment provides a method for the biological sunscreen and barrier repair additive composition, which method includes:

S01 Dissolve the extract of Aquaphora glauca seed oil, Silybum marianum seed oil, and Nannochloropsis oculata extract in ethanol to obtain mixture 1;

S02 mix the remaining raw materials and the lower liquid from step (2) to obtain mixture 2;

S03 can be prepared by applying mixture 1 to the inner wall of the container, drying it, adding mixture 2, and then rotating and shaking at 35°C.

Example 2

This embodiment provides a biological sunscreen and barrier-repairing additive composition, which includes Porphyra lucidum seed oil, Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract. , milk thistle seed oil, Nannochloropsis ocularis extract; and the mass ratio is 3:23:55:0.7:8:0.1.

Wherein, the extraction method of the glabrous water bark seed oil is as follows:

(1) Pre-treatment: collect the hairless water husk seeds, place them in an oven for drying, grind the dried hairless water husk seeds to obtain hairless water husk seeds powder; preheat the oven , and the temperature is 80℃;

(2) Extraction: Place the glabrous water bark seed powder in an extraction bottle, add a mixed solvent according to a material-to-liquid ratio of 1:10, and extract at an ultrasonic power of 200W, an ultrasonic time of 30 minutes, and an ultrasonic temperature of 60°C. After completion, filter to obtain the filtrate and filter residue respectively; wherein, the mixed solvent is a mixed solvent of ethanol and n-propanol with a volume ratio of 2:3; the filtrate is rotary evaporated until it becomes a paste.

The Dunaliella salina extract was purchased from Shaanxi Haibo Biotechnology Co., Ltd.

The Haematococcus pluvialis extract was purchased from Xi'an Muyuan Biotechnology Co., Ltd.

PORPHYRA UMBILICALIS extract was purchased from Kangdu Pharmaceutical Co., Ltd.

The milk thistle seed oil was purchased from Shanxi Wenyun Biotechnology Co., Ltd.

NANNOCHLOROPSIS OCULATA extract was purchased from Wuhan Jiuzhouyuan Biotechnology Co., Ltd.

Another aspect of this embodiment provides a method for the biological sunscreen and barrier repair additive composition, which method includes:

S01 Dissolve the extract of Aquaphora glauca seed oil, Silybum marianum seed oil, and Nannochloropsis oculata extract in ethanol to obtain mixture 1;

S02 mix the remaining raw materials and the lower liquid from step (2) to obtain mixture 2;

S03 can be prepared by applying mixture 1 to the inner wall of the container, drying it, adding mixture 2, and then rotating and shaking at 35°C.

Example 3

The difference from Example 1 is that the preparation method of the composition is as follows: S01 Dissolve the extract of Nannochloropsis glaucoma seed oil, milk thistle seed oil, and Nannochloropsis oculata in ethanol to obtain a mixture 1;

S02 mix the remaining raw materials and the lower liquid from step (2) to obtain mixture 2;

S03 Stir Mixture 1 and Mixture 2 at 300r/min for 5 minutes.

Example 4

The difference from Example 1 is that the composition does not contain Aphrodisiac seed oil.

Example 5

The difference from Example 1 is that when the total amounts of Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract remain unchanged, the Dunaliella salina extract, Haematococcus pluvialis extract, and The ratio of algae extract and Porphyra umbilicus extract was adjusted to 10:20:0.1.

Example 6

The difference from Example 1 is that when the total amounts of Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract remain unchanged, the Dunaliella salina extract, Haematococcus pluvialis extract, and The ratio of algae extract and Porphyra umbilicus extract was adjusted to 30:70:11.

Example 7

The difference from Example 5 is that it does not contain Saccharomyces lucidum seed oil.

Example 8

The difference from Example 6 is that it does not contain Aphrodisiac seed oil.

Performance testing: basic testing methods:

PFA test: Inspection shall be carried out in accordance with the specific requirements of the currently valid technical specifications. The subject was placed in a forward leaning position and his back was irradiated. Predict the minimum darkening amount (MPPD value) of the subject's skin to ultraviolet irradiation 24 hours before the test, and adjust the ultraviolet irradiation amount based on the prediction results to test the object being tested. The test first selects a normal skin area of no less than ^30cm2 on the back of the subject, and evenly applies the test substance or reference substance to the above area at a dosage of (2.00±0.05)mg/ ^cm2 ; then selects UVA irradiation according to the specifications. The amount is irradiated in three situations: ① The subject's skin is not coated with the test object; ② The control substance is applied to the skin; ③ The test object is applied to the skin. Observe the experimental results after 2 hours, and record the MPPD values in the three conditions.

Calculation method of PFA value: The PFA value of a single subject protected by the test substance or reference substance is expressed by the following formula:

The individual PFA value needs to be accurate to one digit after the decimal point. Calculate the arithmetic mean of the PFA values of all subjects protected by the tested object, and take the integer part as the PFA value of the measured sample. To estimate the sampling error of the mean, you can calculate the standard deviation and standard error of the set of data. It is required that the 95% confidence interval (95% CI) of the mean does not exceed 17% of the mean. Increase the number of subjects until the above requirements are met. However, the maximum number shall not exceed 25 people.

SPF test: Inspection is carried out in accordance with the specific requirements of the currently valid technical specifications. The subject was placed in a forward leaning position and his back was irradiated. The minimum erythema amount (MED value) of the subject's skin exposed to ultraviolet radiation is predicted 24 hours before the test, and the ultraviolet radiation dose is adjusted based on the prediction results to test the object being tested. On the day of the test, first select a normal skin area of no less than ^30cm2 on the back of the subject, and apply the test substance or reference substance to be tested evenly in the above area at the dosage of (2.00±0.05)mg/ ^cm2 , and then follow the specifications It is required to select the irradiation dose and perform irradiation in three situations: ① the subject's skin is not coated with the test object; ② the control substance is coated; ③ the test object is coated. Observe the experimental results after 24 hours, and record the MED values in the three conditions.

SPF value calculation method: The SPF value of a single subject protected by the test object or reference substance is expressed by the following formula:

The individual SPF value is required to be accurate to one digit after the decimal point. Calculate the arithmetic mean of the SPF values of all subjects protected by the tested object. The integer part is the SPF value of the measured sample. Estimate the sampling error of the mean to calculate the standard deviation and standard error of the set of data. It is required that the 95% confidence interval (95% CI) of the mean does not exceed 17% of the mean, otherwise the number of subjects should be increased (not more than 25) until the above requirements are met.

Among them, performance test 1 and performance test 2:

The tested object was the sunscreen composition prepared in the Example.

Light source: Daylight simulator xenon arc lamp, all performance indicators meet the requirements of the measurement specifications.

The control substance is the standard product, SPF=20, PFA=8; during the test, the compositions implemented in 1-8 are added to the standard product at a concentration of 0.5%, as test products 1-8.

Test 1: Apply the compositions of test products 1-8 respectively until 15 minutes after the skin is absorbed, and conduct the above-mentioned PFA and SPF tests; among them, add 3 additional sets of tests: apply and absorb on test products 4, 7, and 8 respectively. After that, apply an additional amount of the same amount of Aphrodisiac seed oil as the test product 1 until it is absorbed and then test again, which is designated as test product 9-11.

Test 2: Conduct the above-mentioned PFA and SPF tests immediately after applying the compositions of test products 1-8 respectively; among them, add 3 additional sets of tests: after the test products 4, 7, and 8 are coated and absorbed, additionally apply and The same amount of the hairless aphrodisiac seed oil of Test Product 1 was tested again after absorption, and was designated as Test Product 9-11.

Subjects: A total of 36 people, randomly divided into 12 groups, with an average age of 33.5±4.2 years old, meeting the subject voluntary inclusion criteria. Four sets of test products are tested at the same time behind each person, and each test product or standard product is tested for 12 times.

Table 1 Test results of performance tests 1 and 2

Among them, as shown in Figure 1 is the test comparison chart of Example 2 and Example 6 in the SPF test chart, where MED is the initial test chart, which is used to determine the extent of the subject's body withstanding ultraviolet rays; 0240 is the test of Example 2 Figure, 0241 position embodiment 6 test chart; P2 position positive control chart.

Performance test 3: Use kojic acid as the positive control substance, dilute it with pure water to the positive control substance concentration of 0.1%, the negative control substance is pure water, test article: Example 1-8, dilute it with 95% ethanol to the test concentration of 0.2%.

Operation steps: Set up different reaction tubes, namely sample tube, sample background tube, enzyme reaction tube and solvent background tube. Add different reagent solvents respectively, shake gently, let stand at room temperature for 5 minutes, and move the reaction solution into 1cm In the cuvette, the absorbance value was measured at 475 nm.

Calculate tyrosinase inhibition rate (%) = (1-(TT ₀ )/(CC ₀ )) × 100

in:

T - the absorbance value of the solution after the test product reacts with tyrosinase;

T ₀ - background absorbance value of the test product;

The absorbance value of the C-enzyme reaction tube is averaged three times, that is, the absorbance value of the reaction between tyrosinase and dopa without adding the test product;

C ₀ -solvent background absorbance value.

The test results are as follows:

Table 2 Test results of performance test 3

Among them, the tyrosinase inhibition rate is kept to three decimal places, and P<0.05 means that there is a significant difference between the sample and the positive control and the negative control.

Test 4: Statistics of the subjects’ feelings about the test product in Test 1.

Divided into: refreshing 5 points, moderate 3 points, greasy 1 point.

Table 3 Test results of performance test 4

Example Test 4 results Example 1 15 Example 2 13 Example 3 11 Example 4 11 Example 5 13 Example 6 11 Example 7 9 Example 8 9 Example 9 11 Example 10 7 Example 11 7

Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention, but not to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: The technical solutions described in the foregoing embodiments can still be modified, or some or all of the technical features can be equivalently replaced; and these modifications or substitutions do not deviate from the essence of the corresponding technical solutions from the technical solutions of the embodiments of the present invention. scope.

Claims (10)

1. A biological sun protection and repair barrier additive composition, which is characterized by comprising hairless water yellow skin seed oil, dunaliella salina extract, haematococcus pluvialis extract and umbilical laver extract; and the ratio of the dunaliella salina extract, the haematococcus pluvialis extract and the umbilical laver extract is as follows: (10-30): (20-70): (0.1-1).

2. The biological sun protection and repair barrier additive composition according to claim 1, wherein the extraction method of the hairless water yellow skin seed oil is as follows:

(1) Pretreatment: collecting hairless water wampee seeds, placing the hairless water wampee seeds in an oven for drying, and grinding the dried hairless water wampee seeds to obtain hairless water wampee seed powder;

(2) Extracting: placing wampee seed powder without wool water into a leaching bottle, adding a mixed solvent according to a feed-liquid ratio of 1:10, simultaneously leaching at an ultrasonic power of 200W for 30min at an ultrasonic temperature of 60 ℃, and filtering to obtain filtrate and filter residues respectively after the completion of leaching;

(3) Mixing filter residues and a culture medium for culture, wherein the culture condition is that the culture is carried out for 7 days at the temperature of 27+/-1 ℃, and the obtained culture solution is mixed with the filtrate in the step (2), wherein the strain is bacillus subtilis; the inoculation amount is 6%;

(4) Adding deionized water with the same volume into the mixed solution obtained in the step (3), standing, taking the upper layer liquid, and then rotationally evaporating to paste.

3. The biological sun protection and repair barrier additive composition according to claim 2, wherein the temperature inside the oven is 80 ℃; and the mixed solvent is ethanol and n-propanol with the volume ratio of 2: 3.

4. The biological sun protection and repair barrier additive composition according to claim 1, wherein the ratio of dunaliella salina extract, haematococcus pluvialis extract, umbilical laver extract is: (15-30): (25-70): (0.5-1).

5. The biological sun protection and repair barrier additive composition according to claim 1, wherein the ratio of dunaliella salina extract, haematococcus pluvialis extract, umbilical laver extract is: (20-30): (40-70): (0.5-1).

6. The biologic sun protection and repair barrier additive composition of claim 2, further comprising silybum marianum seed oil, nannochloropsis oculata extract.

7. A biologic sun protection and repair barrier additive composition according to claim 1, wherein said composition is added to daily chemicals at a concentration of 0.1-5 wt%.

8. A method of preparing the biologic sun protection, repair barrier additive composition of claim 6, said method comprising:

s01, dissolving the wampee seed oil, the silybum marianum seed oil and the microphotomae extract of the ocular vein into ethanol to obtain a mixture 1;

s02, mixing the rest raw materials with the lower layer liquid of the step (2) to obtain a mixture 2;

s03, coating the mixture 1 in the inner wall of a container, drying, adding the mixture 2, and then rotating and vibrating at 35 ℃ to obtain the composite material.

9. The use of a biological sun protection, repair barrier additive composition according to any one of claims 1-6 in the field of sun protection, skin repair barriers.

10. A testing method for testing sun protection values is characterized by comprising the following steps:

applying the composition of any one of claims 1-6 to a test site while simultaneously performing gradient ultraviolet irradiation on different test sites of the same subject, and performing calculation of sun protection value after irradiation.