CN116908479B - Full-automatic hemagglutination and hemagglutination inhibition test pipetting device - Google Patents
Full-automatic hemagglutination and hemagglutination inhibition test pipetting device Download PDFInfo
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- CN116908479B CN116908479B CN202310983178.1A CN202310983178A CN116908479B CN 116908479 B CN116908479 B CN 116908479B CN 202310983178 A CN202310983178 A CN 202310983178A CN 116908479 B CN116908479 B CN 116908479B
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- 230000005764 inhibitory process Effects 0.000 title claims abstract description 20
- 238000012360 testing method Methods 0.000 title claims abstract description 14
- 230000035931 haemagglutination Effects 0.000 title description 6
- 239000007788 liquid Substances 0.000 claims abstract description 170
- 230000023555 blood coagulation Effects 0.000 claims abstract description 37
- 238000001125 extrusion Methods 0.000 claims description 20
- 238000001179 sorption measurement Methods 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 238000000605 extraction Methods 0.000 abstract description 17
- 238000002474 experimental method Methods 0.000 abstract description 13
- 238000007789 sealing Methods 0.000 description 8
- 230000009471 action Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000011550 stock solution Substances 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 3
- 235000017491 Bambusa tulda Nutrition 0.000 description 3
- 241001330002 Bambuseae Species 0.000 description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 3
- 239000011425 bamboo Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000005389 magnetism Effects 0.000 description 3
- 101710154606 Hemagglutinin Proteins 0.000 description 2
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 2
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 2
- 101710176177 Protein A56 Proteins 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000000185 hemagglutinin Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010211 hemagglutination inhibition (HI) assay Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of medical equipment, in particular to a full-automatic blood coagulation and blood coagulation inhibition test pipetting device which comprises a cabinet body, wherein a blood coagulation plate and a liquid storage plate are arranged on the upper surface of the cabinet body, support rods which are vertically arranged are arranged on two sides of the cabinet body, a fixed plate is arranged between the support rods, a chute is arranged on one side, close to the cabinet body, of the fixed plate, a telescopic cylinder is connected in a sliding manner in the chute, and the lower end of the telescopic cylinder is connected with an adjustable pipetting structure; after the first pin rod and the second pin rod are inserted into the corresponding pin holes, the first permanent magnet and the second permanent magnet are driven to move upwards by virtue of the upward movement of the transverse plate, when the first permanent magnet and the second permanent magnet move upwards to a certain position respectively, the step-type extraction of liquid in the liquid storage plate can be completed, the pipetting convenience of the step concentration experiment is improved, single pipetting is not required to be repeated, and the pipetting efficiency is further improved.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a full-automatic blood coagulation and blood coagulation inhibition test pipetting device.
Background
Viruses of Hemagglutinin (HA) can agglutinate human or animal erythrocytes, known as hemagglutination, which can be inhibited by the corresponding antibodies, known as hemagglutination inhibition assays;
At present, PBS, chicken erythrocyte suspension, abbe solution, 4 unit antigen and the like are required to be added according to a certain sequence in the blood coagulation inhibition experiment process, so that continuous pipetting operation is required, repeated pipetting operation is usually carried out manually in the current blood coagulation inhibition experiment, step concentration reagent is configured for experiment, and the manual pipetting operation effect in the experiment process is poor in convenience and lower in efficiency, so that the full-automatic blood coagulation and blood coagulation inhibition experiment pipetting device is provided for improving pipetting convenience and pipetting efficiency in the blood coagulation inhibition experiment process.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a full-automatic blood coagulation and blood coagulation inhibition test pipetting device which is used for improving pipetting convenience and pipetting efficiency in the blood coagulation inhibition test process.
The technical scheme adopted by the invention for solving the technical problems is that the full-automatic blood coagulation and blood coagulation inhibition test pipetting device comprises a cabinet body, wherein a blood coagulation plate and a liquid storage plate are arranged on the upper surface of the cabinet body, support rods which are vertically arranged are arranged on two sides of the cabinet body, a fixed plate is arranged between the support rods, a chute is arranged on one side, close to the cabinet body, of the fixed plate, a telescopic cylinder is connected in a sliding manner in the chute, and an adjustable pipetting structure is connected to the lower end of the telescopic cylinder.
Through adopting above-mentioned technical scheme, rely on flexible jar to slide in the spout and connect to and flexible jar flexible removal drives adjustable pipetting structure and carries out synchronous movement, relies on adjustable pipetting structure to carry out the extraction with the liquid in the stock solution board, and with liquid storage in adjustable pipetting structure, again through the sliding connection of flexible jar and spout this moment, be convenient for carry the liquid of storage in adjustable pipetting structure on the hemagglutination board, thereby improve pipetting convenience, and pipetting efficiency.
Specifically, adjustable pipetting structure includes the casing, be equipped with the diaphragm that the level set up in the casing, electric telescopic handle is installed on the upper portion of casing, electric telescopic handle's expansion end passes the casing and is connected with diaphragm upper surface, diaphragm below is equipped with first location structure, second location structure, be equipped with adjustable drive structure between first location structure and the second location structure, the casing lower surface is equipped with a plurality of group pipetting cartridge.
Through adopting above-mentioned technical scheme, when the telescopic cylinder slides to certain position in the spout, drive adjustable pipetting structure and move down by means of the telescopic cylinder, and make the casing be located the stock solution board top, when the casing moves down to certain position, a plurality of group pipetting cylinder is corresponding with the stock solution board, it moves the upward movement to rely on electric telescopic handle to drive the diaphragm this moment, drive first location structure when the diaphragm moves upward, second location structure and adjustable drive structure move upward, and rely on first location structure, second location structure and adjustable drive structure to make pipetting cylinder carry out the extraction to the liquid in the stock solution board.
Specifically, the first positioning structure comprises a first permanent magnet, the upper surface of the first permanent magnet is magnetically adsorbed with the lower surface of the transverse plate, a first piston rod which is vertically arranged is arranged below the first permanent magnet, one end of the first piston rod, which is far away from the first permanent magnet, penetrates through the shell and is positioned in the pipetting cylinder, a first piston is fixedly connected to the lower part, which is far away from the first permanent magnet, of the first piston rod, and the first piston is in sealed sliding connection with the inside of the pipetting cylinder;
The second positioning structure comprises a second permanent magnet, the upper surface of the second permanent magnet is fixedly connected with the lower surface of the transverse plate, a second piston rod which is vertically arranged is arranged below the second permanent magnet, one end of the second piston rod, which is far away from the second permanent magnet, penetrates through the shell and is positioned in the pipetting cylinder, a second piston is fixedly connected with the lower part of the second piston rod, which is far away from the second permanent magnet, and the second piston is in sealed sliding connection with the inside of the pipetting cylinder;
The adjustable driving structure comprises a moving plate, the outside of first permanent magnet, second permanent magnet is all fixedly connected with first articulated shaft, second articulated shaft, the moving plate is located between first permanent magnet, the second permanent magnet, the connecting plate is all installed around the moving plate, two sets of connecting plates are close to the one end of first permanent magnet and all articulate and connect first articulated shaft, two sets of connecting plates are close to the one end of second permanent magnet and all are equipped with the opening, opening and second articulated shaft sliding connection, the lower part of moving plate is equipped with the sliding tray, equidistant sliding connection has a plurality of groups of sliders in the sliding tray, the lower part of slider all articulates there is the third piston rod, the one end that the moving plate was kept away from to the third piston rod all passes the casing and is located a plurality of groups of liquid transfer section of thick bamboo respectively, the below that the moving plate was kept away from to the third piston rod is all fixedly connected with third piston, third piston and the inside sealed sliding connection of liquid transfer section of thick bamboo.
By adopting the technical scheme, when the electric telescopic rod drives the transverse plate to move upwards, the first permanent magnet and the transverse plate are adsorbed by magnetism, and the second permanent magnet and the transverse plate are fixedly connected, the first permanent magnet and the second permanent magnet are driven to move upwards synchronously when the transverse plate moves upwards, the first piston rod and the first piston are driven to move upwards synchronously when the first permanent magnet moves upwards, the second piston rod and the second piston are driven to move upwards synchronously when the second permanent magnet moves upwards, and when the first piston and the second piston move upwards, liquid in the liquid storage plate is pumped into the liquid transfer cylinder through the liquid transfer cylinder;
When the first permanent magnet and the second permanent magnet synchronously move upwards, the first hinge shaft and the second hinge shaft are used for driving the movable plate to synchronously move upwards, when the movable plate moves upwards, the sliding block is used for driving the third piston rod and the third piston to synchronously move upwards, and when the third piston moves upwards, the liquid in the liquid storage plate is pumped into the liquid storage cylinder through the liquid storage cylinder, so that the liquid in the liquid storage plate is conveniently pumped;
The sliding connection of the sliding block and the sliding groove can improve the moving convenience and stability of the third piston rod, and meanwhile, the opening arranged at one end of the second permanent magnet can improve the moving convenience and stability of the moving plate.
Specifically, a first adjusting plate and a second adjusting plate which are vertically arranged are respectively arranged on two sides of the inside of the shell, and a plurality of groups of pin holes which are vertically distributed are respectively arranged on the first adjusting plate and the second adjusting plate;
The shell is provided with a first grooving and a second grooving on one side close to the support rod, the first grooving and the second grooving correspond to the first adjusting plate and the second adjusting plate respectively, a first pin rod and a second pin rod are connected in the first grooving and the second grooving in a sliding mode respectively, one end of the first pin rod penetrates through the pin hole and contacts with the upper surface of the first permanent magnet and is magnetically adsorbed, and one end of the second pin rod penetrates through the pin hole and contacts with the upper surface of the second permanent magnet.
By adopting the technical scheme, when the extraction amount of liquid is required to be adjusted in a stepwise manner, the lowest extraction amount of the liquid is determined by moving the first pin rod to a proper position and inserting the first pin rod into the pin hole on the first adjusting plate; simultaneously, the second pin rod is moved to a proper position, and is inserted into a pin hole on the second adjusting plate, so that the maximum liquid extraction amount is determined;
it should be noted that the insertion height of the second pin needs to be higher than that of the first pin;
when the first pin rod and the second pin rod are inserted into the corresponding pin holes, the transverse plate is driven by the electric telescopic rod to move upwards, the first permanent magnet and the second permanent magnet are driven to move upwards synchronously when the transverse plate moves upwards, when the first permanent magnet moves upwards to a certain position, the liquid in the liquid storage plate is extracted by virtue of the sealed sliding connection of the first piston and the liquid moving cylinder, the first permanent magnet cannot move upwards by virtue of the action of the first pin rod, when the transverse plate moves upwards continuously, the transverse plate is separated from the first permanent magnet at the moment and does not drive the first permanent magnet to move upwards any more, the first permanent magnet can be prevented from automatically falling to drive the first piston rod to move downwards by virtue of the magnetic adsorption of the upper surface of the first pin rod and the first permanent magnet, and the liquid in the liquid moving cylinder is extruded;
When the transverse plate moves upwards continuously, the second permanent magnet is driven to move upwards, when the second permanent magnet moves upwards, the moving plate is driven to move upwards, and as the first permanent magnet cannot move upwards, when the moving plate moves upwards, the moving plate is rotated, a plurality of groups of third piston rods and third pistons are driven to move upwards by virtue of the rotation of the moving plate, and liquid in the liquid storage plate is extracted by virtue of the sealing sliding connection of the third pistons and the liquid moving cylinder;
When the second permanent magnet moves up to a certain position, the liquid in the liquid storage plate is extracted by means of the sealing sliding connection of the second piston and the liquid-moving cylinder, and meanwhile, the second permanent magnet cannot move up by means of the contact of the second pin rod and the second permanent magnet, so that step-type extraction of the liquid in the liquid storage plate is completed, the liquid-moving convenience in step concentration experiments is improved, single liquid-moving is not required to be repeatedly performed, and the liquid-moving efficiency is further improved.
Specifically, the lower extreme of a plurality of group's pipettes section of thick bamboo all is equipped with the liquid taking head, the lower extreme of liquid taking head all can be dismantled and be connected with the suction head, a plurality of groups liquid taking head outside all is established sliding connection and is had the stripper plate, be equipped with a plurality of groups trompil on the stripper plate, trompil and liquid taking head sliding connection, the casing below is equipped with extrusion structure, extrusion structure and stripper plate fixed connection.
By adopting the technical scheme, when the liquid in the liquid storage plate is required to be extracted, the suction head is arranged on the liquid taking head of the liquid transferring cylinder, and the suction head is placed on the liquid in the liquid storage plate, so that the liquid in the liquid storage plate is extracted into the liquid transferring cylinder by means of the suction head; rely on the fixed connection of extrusion structure and stripper plate, when the drive extrusion structure, be convenient for separate suction head and liquid head to improve convenience and the change efficiency of changing the suction head.
Specifically, the extrusion structure comprises two groups of fixed cylinders arranged on two sides of the lower surface of the shell, electromagnets are arranged in the two groups of fixed cylinders, the upper surfaces of the two groups of electromagnets respectively correspond to the lower surfaces of the first permanent magnet and the second permanent magnet, driving rods are fixedly connected to the lower surfaces of the two groups of electromagnets, one ends of the driving rods, far away from the electromagnets, penetrate through the fixed cylinders and are in sliding connection with the fixed cylinders, and the lower ends of the driving rods are respectively fixedly connected with the upper surface of the extrusion plate;
The fixed cylinder is internally provided with a reset spring, one end of the reset spring is fixedly connected with the lower surface of the electromagnet, and the other end of the reset spring is fixedly connected with the inner bottom of the fixed cylinder.
By adopting the technical scheme, when the transverse plate moves downwards, the first permanent magnet and the second permanent magnet are driven to move downwards, and when the first permanent magnet and the second permanent magnet move downwards to a certain position, liquid in the liquid transferring cylinder is extruded and discharged, and at the moment, the first permanent magnet and the second permanent magnet are respectively close to the electromagnet;
When the liquid in the liquid transferring cylinder is discharged and needs to be replaced, the electromagnet is driven to move downwards by means of repulsive force generated by the electromagnet and the first permanent magnet and the second permanent magnet respectively, the reset spring is driven to store force when the electromagnet moves downwards, meanwhile, the extrusion plate is driven to move downwards by means of the downward movement of the electromagnet, and when the extrusion plate moves downwards, the suction head on the liquid taking head is extruded by means of sliding connection of the opening and the liquid taking head, and the suction head is separated from the liquid taking head, so that the suction head is convenient to replace;
When the suction head is separated from the liquid taking head, the electromagnet is closed, and the electromagnet is driven to move upwards under the action of the reset spring, so that the electromagnet is restored to an initial state, and at the moment, a new suction head can be installed on the liquid taking head, so that convenience in replacement of the suction head and replacement efficiency are improved.
The invention has the beneficial effects that:
(1) According to the full-automatic blood coagulation and blood coagulation inhibition test pipetting device, after the first pin rod and the second pin rod are inserted into the corresponding pin holes, the first permanent magnet and the second permanent magnet are driven to move upwards by virtue of the upward movement of the transverse plate, when the first permanent magnet and the second permanent magnet move to certain positions respectively, the step-type extraction of liquid in the liquid storage plate can be completed, the pipetting convenience in step concentration experiments is improved, single pipetting is not required to be repeated, and the pipetting efficiency is further improved.
(2) According to the full-automatic blood coagulation and blood coagulation inhibition test pipetting device, when liquid in a pipetting cylinder is drained and a pipette head needs to be replaced, the electromagnet is driven to move downwards by means of repulsive force generated by the electromagnet and the first permanent magnet and the second permanent magnet respectively, the squeeze plate is driven to move downwards by means of the downward movement of the electromagnet, and when the squeeze plate moves downwards, the pipette head on the pipetting head is squeezed by means of sliding connection of the opening and the pipetting head, and the pipette head is separated from the pipetting head, so that the pipette head is convenient to replace.
(3) According to the full-automatic blood coagulation and blood coagulation inhibition test pipetting device, the first permanent magnet can be prevented from automatically falling down to drive the first piston rod to move down by virtue of magnetic adsorption of the first pin rod and the upper surface of the first permanent magnet, so that liquid in the pipetting cylinder is extruded out, and the using effect of the full-automatic blood coagulation and blood coagulation inhibition test pipetting device is improved.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is an isometric view of a first embodiment of the invention;
FIG. 2 is an isometric view of a housing of the present invention;
FIG. 3 is a schematic cross-sectional view of a housing according to the present invention;
FIG. 4 is a schematic view of the connection structure of the transverse plate after being moved up;
FIG. 5 is a schematic view showing the internal structure of the shell during step liquid taking according to the present invention;
FIG. 6 is a schematic view of a connection structure of a connection board according to the present invention;
FIG. 7 is a schematic view of the bottom structure of the mobile plate of the present invention;
Fig. 8 is a schematic view showing a connection structure of a squeeze plate according to a second embodiment of the present invention;
FIG. 9 is a schematic view of a connecting structure of a fixed cylinder according to a second embodiment of the present invention;
FIG. 10 is an enlarged schematic view of the area A of FIG. 9 according to the present invention;
In the figure, 1, a cabinet body; 2. a blood coagulation plate; 3. a liquid storage plate; 4. a support rod; 5. a fixing plate; 6. a first permanent magnet; 7. a housing; 8. a cross plate; 9. an electric telescopic rod; 10. a pipetting cylinder; 11. a first piston rod; 12. a first piston; 13. a second permanent magnet; 14. a second piston rod; 15. a second piston; 16. a first hinge shaft; 17. a second hinge shaft; 18. a moving plate; 19. a connecting plate; 20. an opening; 21. a sliding groove; 22. a slide block; 23. a third piston rod; 24. a third piston; 25. a first adjustment plate; 26. a second adjusting plate; 27. a pin hole; 28. a first slot; 29. a second slot; 30. a first pin; 31. a second pin; 32. a liquid taking head; 33. a suction head; 34. an extrusion plate; 35. a fixed cylinder; 36. an electromagnet; 37. a driving rod; 38. a return spring; 39. and a telescopic cylinder.
Detailed Description
The invention is further described in connection with the following detailed description in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
In order to improve the convenience and the efficiency of pipetting in the process of the experiment of blood coagulation inhibition, as shown in fig. 1 and 2, as a first embodiment of the invention, the full-automatic blood coagulation and blood coagulation inhibition experiment pipetting device comprises a cabinet body 1, wherein the upper surface of the cabinet body 1 is provided with blood coagulation plates 2 and liquid storage plates 3, two sides of the cabinet body 1 are respectively provided with a support rod 4 which is vertically arranged, a fixing plate 5 is arranged between the support rods 4, one side of the fixing plate 5, which is close to the cabinet body 1, is provided with a chute, a telescopic cylinder 39 is connected in the chute in a sliding manner, and the lower end of the telescopic cylinder 39 is connected with an adjustable pipetting structure.
When the adjustable liquid-transfering device is used, the liquid in the liquid-transfering plate 3 is extracted by means of the adjustable liquid-transfering structure and stored in the adjustable liquid-transfering structure by means of the telescopic cylinder 39 in sliding connection with the sliding chute and the telescopic cylinder 39 in telescopic movement, so that the liquid stored in the adjustable liquid-transfering structure is conveniently conveyed on the blood coagulation plate 2 by means of the sliding connection of the telescopic cylinder 39, and the liquid-transfering convenience and the liquid-transfering efficiency are improved.
In order to facilitate the extraction of the liquid in the liquid storage plate 3, as shown in fig. 2 and 3, the invention further comprises that the adjustable liquid-transferring structure comprises a shell 7, a horizontal transverse plate 8 is arranged in the shell 7, an electric telescopic rod 9 is arranged at the upper part of the shell 7, the movable end of the electric telescopic rod 9 penetrates through the shell 7 and is connected with the upper surface of the transverse plate 8, a first positioning structure and a second positioning structure are arranged below the transverse plate 8, an adjustable driving structure is arranged between the first positioning structure and the second positioning structure, and a plurality of groups of liquid-transferring cylinders 10 are arranged on the lower surface of the shell 7.
When in use, when the telescopic cylinder 39 slides to a certain position in the sliding groove, the telescopic cylinder 39 is used for driving the adjustable pipetting structure to move downwards, the shell 7 is positioned above the liquid storage plate 3, when the shell 7 moves downwards to a certain position, the pipetting cylinders 10 of the plurality of groups are corresponding to the liquid storage plate 3, the transverse plate 8 is driven to move upwards by the electric telescopic rod 9, the first positioning structure, the second positioning structure and the adjustable driving structure are driven to move upwards when the transverse plate 8 moves upwards, and the pipetting cylinders 10 are used for drawing liquid in the liquid storage plate 3 by the first positioning structure, the second positioning structure and the adjustable driving structure.
In order to enable the synchronous extraction of the liquid in the plurality of groups of pipetting cylinders 10, as shown in fig. 3, 4, 6 and 7, the invention further comprises a first positioning structure, wherein the first positioning structure comprises a first permanent magnet 6, the upper surface of the first permanent magnet 6 is magnetically adsorbed to the lower surface of a transverse plate 8, a first piston rod 11 which is vertically arranged is arranged below the first permanent magnet 6, one end of the first piston rod 11, which is far away from the first permanent magnet 6, penetrates through the shell 7 and is positioned in the pipetting cylinder 10, a first piston 12 is fixedly connected below the first piston rod 11, which is far away from the first permanent magnet 6, and the first piston 12 is in sealed sliding connection with the inside of the pipetting cylinder 10;
The second positioning structure comprises a second permanent magnet 13, the upper surface of the second permanent magnet 13 is fixedly connected with the lower surface of the transverse plate 8, a second piston rod 14 which is vertically arranged is arranged below the second permanent magnet 13, one end of the second piston rod 14, which is far away from the second permanent magnet 13, passes through the shell 7 and is positioned in the pipetting cylinder 10, a second piston 15 is fixedly connected below the second piston rod 14, which is far away from the second permanent magnet 13, and the second piston 15 is in sealed sliding connection with the interior of the pipetting cylinder 10;
The adjustable driving structure comprises a moving plate 18, the outer sides of a first permanent magnet 6 and a second permanent magnet 13 are fixedly connected with a first hinging shaft 16 and a second hinging shaft 17, the moving plate 18 is positioned between the first permanent magnet 6 and the second permanent magnet 13, connecting plates 19 are respectively installed at the front and back of the moving plate 18, one ends of two groups of connecting plates 19, which are close to the first permanent magnet 6, are respectively hinged with the first hinging shaft 16, one ends of two groups of connecting plates 19, which are close to the second permanent magnet 13, are respectively provided with an opening 20, the openings 20 are in sliding connection with the second hinging shaft 17, the lower part of the moving plate 18 is provided with a sliding groove 21, a plurality of groups of sliding blocks 22 are in equidistant sliding connection in the sliding groove 21, the lower parts of the sliding blocks 22 are respectively hinged with a third piston rod 23, one ends of the third piston 24 rods 23, which are far away from the moving plate 18, are respectively positioned in a plurality of groups of liquid moving cylinders 10, the lower parts of the third piston 24 rods 23, which are far away from the moving plate 18, are respectively fixedly connected with a third piston 24, and the inside the liquid moving cylinders 10 are in a sealing sliding connection.
When in use, when the electric telescopic rod 9 drives the transverse plate 8 to move upwards, the first permanent magnet 6 and the transverse plate 8 are adsorbed by magnetism, and the second permanent magnet 13 is fixedly connected with the transverse plate 8, when the transverse plate 8 moves upwards, the first permanent magnet 6 and the second permanent magnet 13 are driven to synchronously move upwards, when the first permanent magnet 6 moves upwards, the first piston rod 11 and the first piston 12 are driven to synchronously move upwards, when the second permanent magnet 13 moves upwards, the second piston rod 14 and the second piston 15 are driven to synchronously move upwards, and when the first piston 12 and the second piston 15 move upwards, the liquid in the liquid storage box is pumped into the liquid moving cylinder 10 through the liquid moving cylinder 10;
When the first permanent magnet 6 and the second permanent magnet 13 synchronously move upwards, the first hinging shaft 16 and the second hinging shaft 17 drive the moving plate 18 to synchronously move upwards, when the moving plate 18 moves upwards, the sliding block 22 drives the third piston rod 23 and the third piston 24 to synchronously move upwards, and when the third piston 24 moves upwards, the liquid in the liquid storage box is pumped into the liquid storage barrel 10 through the liquid storage barrel 10, so that the liquid in the liquid storage box is conveniently pumped;
The sliding connection of the sliding block 22 and the sliding groove 21 can improve the moving convenience and stability of the third piston rod 23, and meanwhile, the opening 20 arranged at one end of the second permanent magnet 13 can improve the moving convenience and stability of the moving plate 18.
In order to complete the extraction of the liquid in the liquid storage plate 3 during the step, as shown in fig. 3,4 and 5, the invention further comprises, for example, a first adjusting plate 25 and a second adjusting plate 26 which are vertically arranged on two sides of the interior of the housing 7, wherein a plurality of groups of pin holes 27 which are vertically distributed are respectively arranged on the first adjusting plate 25 and the second adjusting plate 26;
The shell 7 is close to one side of bracing piece 4 and is equipped with first fluting 28, second fluting 29 respectively, first fluting 28, second fluting 29 respectively correspond with first regulating plate 25, second regulating plate 26, first fluting 28, second fluting 29 are respectively sliding connection has first pin 30, second pin 31, the one end of first pin 30 passes pinhole 27 and contacts and magnetic adsorption with first permanent magnet 6 upper surface, the one end of second pin 31 passes pinhole 27 and contacts with second permanent magnet 13 upper surface.
In use, when a step adjustment is required to be made to the amount of liquid extracted, the minimum amount of liquid extracted is determined by moving the first pin 30 to a proper position and inserting the first pin 30 into the pin hole 27 on the first adjustment plate 25; at the same time, the maximum liquid extraction amount is determined by moving the second pin 31 to a proper position and inserting the second pin 31 into the pin hole 27 on the second adjusting plate 26;
It should be noted that the insertion height of the second pin 31 needs to be higher than that of the first pin 30;
When the first pin rod 30 and the second pin rod 31 are inserted into the corresponding pin holes 27, the transverse plate 8 is driven by the electric telescopic rod 9 to move upwards, the first permanent magnet 6 and the second permanent magnet 13 are driven to synchronously move upwards when the transverse plate 8 moves upwards, when the first permanent magnet 6 moves upwards to a certain position, the first piston 12 is connected with the pipetting cylinder 10 in a sealing sliding way to pump the liquid in the liquid storage plate 3, the first permanent magnet 6 cannot move upwards under the action of the first pin rod 30, when the transverse plate 8 moves upwards continuously, the transverse plate 8 is separated from the first permanent magnet 6 at the moment and does not drive the first permanent magnet 6 to move upwards any more, and the first permanent magnet 6 can be prevented from automatically falling to drive the first piston 12 to move downwards by virtue of the magnetic adsorption of the upper surfaces of the first pin rod 30 and the first permanent magnet 6, so that the liquid in the pipetting cylinder 10 is extruded;
When the transverse plate 8 continues to move upwards, the second permanent magnet 13 is driven to move upwards, when the second permanent magnet 13 moves upwards, the moving plate 18 is driven to move upwards, because the first permanent magnet 6 cannot move upwards, when the moving plate 18 moves upwards, the moving plate 18 rotates, a plurality of groups of third piston 24 rods 23 and third pistons 24 are driven to move upwards by virtue of the rotation of the moving plate 18, and liquid in the liquid storage plate 3 is extracted by virtue of the sealing sliding connection of the third pistons 24 and the liquid storage cylinder 10;
when the second permanent magnet 13 moves up to a certain position, the liquid in the liquid storage plate 3 is extracted by virtue of the sealing sliding connection of the second piston 15 and the liquid transferring cylinder 10, and meanwhile, the second permanent magnet 13 cannot move up by virtue of the contact of the second pin rod 31 and the second permanent magnet 13, so that the step-type extraction of the liquid in the liquid storage plate 3 is completed, the liquid transferring convenience in the step concentration experiment is improved, the repeated single liquid transferring is not needed, and the liquid transferring efficiency is further improved.
In order to facilitate replacement of the suction head 33, as shown in fig. 8 and 9, as a second embodiment of the present invention, the present invention further includes, at the lower ends of the plurality of groups of pipetting cylinders 10, liquid taking heads 32, wherein the lower ends of the liquid taking heads 32 are detachably connected with the suction head 33, the outer sides of the plurality of groups of liquid taking heads 32 are slidably connected with a squeeze plate 34, the squeeze plate 34 is provided with a plurality of groups of openings, the openings are slidably connected with the liquid taking heads 32, and an extrusion structure is arranged below the housing 7 and fixedly connected with the squeeze plate 34.
When the liquid in the liquid storage plate 3 is required to be extracted in use, the suction head 33 is arranged on the liquid taking head 32 of the liquid transferring cylinder 10, and the suction head 33 is arranged on the liquid in the liquid storage plate 3, so that the liquid in the liquid storage plate 3 is extracted into the liquid transferring cylinder 10 by the suction head 33; by means of the fixed connection between the extrusion structure and the extrusion plate 34, when the extrusion structure is driven, the suction head 33 and the liquid taking head 32 are conveniently separated, so that convenience and replacement efficiency of the suction head 33 are improved.
In order to improve the convenience of replacing the suction head 33, as shown in fig. 9 and 10, the invention further includes, for example, that the extrusion structure includes two groups of fixing cylinders 35 disposed on two sides of the lower surface of the housing 7, wherein the two groups of fixing cylinders 35 are each provided with an electromagnet 36, the upper surfaces of the two groups of electromagnets 36 respectively correspond to the lower surfaces of the first permanent magnet 6 and the second permanent magnet 13, the lower surfaces of the two groups of electromagnets 36 are each fixedly connected with a driving rod 37, one ends of the driving rods 37, which are far away from the electromagnets 36, respectively pass through the fixing cylinders 35 and are in sliding connection with the fixing cylinders 35, and the lower ends of the driving rods 37 are respectively fixedly connected with the upper surfaces of the extrusion plates 34;
the fixed cylinder 35 is internally provided with a return spring 38, one end of the return spring 38 is fixedly connected with the lower surface of the electromagnet 36, and the other end of the return spring 38 is fixedly connected with the inner bottom of the fixed cylinder 35.
When in use, when the transverse plate 8 moves downwards, the first permanent magnet 6 and the second permanent magnet 13 are driven to move downwards, and after the first permanent magnet 6 and the second permanent magnet 13 move downwards to a certain position, liquid in the pipetting cylinder 10 is extruded and drained, and at the moment, the first permanent magnet 6 and the second permanent magnet 13 are respectively close to the electromagnet 36;
When the liquid in the liquid transferring cylinder 10 is discharged and the suction head 33 needs to be replaced, the electromagnet 36 is driven to move downwards by means of repulsive force generated by the electromagnet 36 and the first permanent magnet 6 and the second permanent magnet 13 respectively, the electromagnet 36 is driven to store force by the reset spring 38 when moving downwards, meanwhile, the extrusion plate 34 is driven to move downwards by means of the downward movement of the electromagnet 36, and when the extrusion plate 34 moves downwards, the suction head 33 on the liquid taking head 32 is extruded by means of sliding connection of the opening and the liquid taking head 32, and the suction head 33 is separated from the liquid taking head 32, so that the suction head 33 is convenient to replace;
when the suction head 33 is separated from the liquid taking head 32, the electromagnet 36 is closed, and the electromagnet 36 is driven to move upwards under the action of the reset spring 38, so that the electromagnet 36 is restored to the initial state, and at the moment, a new suction head 33 can be arranged on the liquid taking head 32, so that the convenience of replacing the suction head 33 and the replacement efficiency are improved.
When the invention is used, the telescopic cylinder 39 is in sliding connection in the sliding groove, the telescopic cylinder 39 stretches and moves to drive the shell 7 to move to a proper position, the shell 7 is positioned above the liquid storage plate 3, when the shell 7 moves to a certain position downwards, the groups of liquid moving cylinders 10 correspond to the liquid storage plate 3, the transverse plate 8 is driven to move upwards by the electric telescopic rod 9, when the transverse plate 8 moves upwards, the first permanent magnet 6 and the transverse plate 8 are adsorbed by magnetism, and the second permanent magnet 13 is fixedly connected with the transverse plate 8, when the transverse plate 8 moves upwards, the first permanent magnet 6 and the second permanent magnet 13 are driven to synchronously move upwards, when the first permanent magnet 6 moves upwards, the first piston rod 11 and the first piston 12 are driven to synchronously move upwards, and when the second permanent magnet 13 moves upwards, the second piston rod 14 and the second piston 15 are driven to synchronously move upwards, and when the first piston 12 and the second piston 15 move upwards, liquid in the liquid moving cylinder 3 is pumped into the liquid moving cylinder 10 by the liquid moving cylinder 10;
When the first permanent magnet 6 and the second permanent magnet 13 synchronously move upwards, the first hinging shaft 16 and the second hinging shaft 17 drive the moving plate 18 to synchronously move upwards, when the moving plate 18 moves upwards, the sliding block 22 drives the third piston rod 23 and the third piston 24 to synchronously move upwards, and when the third piston 24 moves upwards, the liquid in the liquid storage plate 3 is pumped into the liquid storage cylinder 10 through the liquid storage cylinder 10, so that the liquid in the liquid storage plate 3 is conveniently pumped;
When the liquid extraction amount is required to be adjusted stepwise, the lowest liquid extraction amount is determined by moving the first pin 30 to a proper position and inserting the first pin 30 into the pin hole 27 on the first adjusting plate 25; at the same time, the maximum liquid extraction amount is determined by moving the second pin 31 to a proper position and inserting the second pin 31 into the pin hole 27 on the second adjusting plate 26;
When the first pin rod 30 and the second pin rod 31 are inserted into the corresponding pin holes 27, the transverse plate 8 is driven by the electric telescopic rod 9 to move upwards, the first permanent magnet 6 and the second permanent magnet 13 are driven to synchronously move upwards when the transverse plate 8 moves upwards, when the first permanent magnet 6 moves upwards to a certain position, the first piston 12 is connected with the pipetting cylinder 10 in a sealing sliding way to pump the liquid in the liquid storage plate 3, the first permanent magnet 6 cannot move upwards under the action of the first pin rod 30, when the transverse plate 8 moves upwards continuously, the transverse plate 8 is separated from the first permanent magnet 6 at the moment and does not drive the first permanent magnet 6 to move upwards any more, and the first permanent magnet 6 can be prevented from automatically falling to drive the first piston 12 to move downwards by virtue of the magnetic adsorption of the upper surfaces of the first pin rod 30 and the first permanent magnet 6, so that the liquid in the pipetting cylinder 10 is extruded;
When the transverse plate 8 continues to move upwards, the second permanent magnet 13 is driven to move upwards, when the second permanent magnet 13 moves upwards, the moving plate 18 is driven to move upwards, because the first permanent magnet 6 cannot move upwards, when the moving plate 18 moves upwards, the moving plate 18 rotates, a plurality of groups of third piston 24 rods 23 and third pistons 24 are driven to move upwards by virtue of the rotation of the moving plate 18, and liquid in the liquid storage plate 3 is extracted by virtue of the sealing sliding connection of the third pistons 24 and the liquid storage cylinder 10;
When the second permanent magnet 13 moves up to a certain position, the liquid in the liquid storage plate 3 is extracted by virtue of the sealed sliding connection of the second piston 15 and the liquid transferring cylinder 10, and meanwhile, the second permanent magnet 13 cannot move up by virtue of the contact of the second pin rod 31 and the second permanent magnet 13, so that the step-type extraction of the liquid in the liquid storage plate 3 is completed, the liquid transferring convenience in the step concentration experiment is improved, the repeated single liquid transferring is not needed, and the liquid transferring efficiency is further improved;
The liquid in the liquid transferring cylinder 10 is extruded and discharged by means of the downward movement of the transverse plate 8, at the moment, when the suction head 33 needs to be replaced, the electromagnet 36 is driven to move downwards by means of repulsive force generated by the electromagnet 36 and the first permanent magnet 6 and the second permanent magnet 13 respectively, when the electromagnet 36 moves downwards, the reset spring 38 is driven to store force, meanwhile, the extruding plate 34 is driven to move downwards by means of the downward movement of the electromagnet 36, and when the extruding plate 34 moves downwards, the suction head 33 on the liquid taking head 32 is extruded by means of sliding connection of the opening and the liquid taking head 32, and the suction head 33 is separated from the liquid taking head 32, so that the suction head 33 is convenient to replace;
when the suction head 33 is separated from the liquid taking head 32, the electromagnet 36 is closed, and the electromagnet 36 is driven to move upwards under the action of the reset spring 38, so that the electromagnet 36 is restored to the initial state, and at the moment, a new suction head 33 can be arranged on the liquid taking head 32, so that the convenience of replacing the suction head 33 and the replacement efficiency are improved.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the foregoing examples, and that the foregoing description and description are merely illustrative of the principles of this invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. The full-automatic blood coagulation and blood coagulation inhibition test pipetting device is characterized by comprising a cabinet body (1), wherein a blood coagulation plate (2) and a liquid storage plate (3) are arranged on the upper surface of the cabinet body (1), support rods (4) which are vertically arranged are arranged on two sides of the cabinet body (1), a fixed plate (5) is arranged between the support rods (4), a chute is arranged on one side, close to the cabinet body (1), of the fixed plate (5), a telescopic cylinder (39) is connected in a sliding manner in the chute, and an adjustable pipetting structure is connected to the lower end of the telescopic cylinder (39);
the adjustable pipetting structure comprises a shell (7), a horizontal plate (8) is arranged in the shell (7), an electric telescopic rod (9) is arranged on the upper portion of the shell (7), the movable end of the electric telescopic rod (9) penetrates through the shell (7) and is connected with the upper surface of the horizontal plate (8), a first positioning structure and a second positioning structure are arranged below the horizontal plate (8), an adjustable driving structure is arranged between the first positioning structure and the second positioning structure, and a plurality of groups of pipetting cylinders (10) are arranged on the lower surface of the shell (7);
the first positioning structure comprises a first permanent magnet (6), the upper surface of the first permanent magnet (6) is magnetically adsorbed with the lower surface of the transverse plate (8), a first piston rod (11) which is vertically arranged is arranged below the first permanent magnet (6), one end of the first piston rod (11) far away from the first permanent magnet (6) penetrates through the shell (7) and is positioned in the pipetting cylinder (10), a first piston (12) is fixedly connected below the first piston rod (11) far away from the first permanent magnet (6), and the first piston (12) is in sealed sliding connection with the inside of the pipetting cylinder (10);
The second positioning structure comprises a second permanent magnet (13), the upper surface of the second permanent magnet (13) is fixedly connected with the lower surface of the transverse plate (8), a second piston rod (14) which is vertically arranged is arranged below the second permanent magnet (13), one end of the second piston rod (14) far away from the second permanent magnet (13) penetrates through the shell (7) and is positioned in the pipetting cylinder (10), a second piston (15) is fixedly connected below the second piston rod (14) far away from the second permanent magnet (13), and the second piston (15) is in sealed sliding connection with the inside of the pipetting cylinder (10);
The adjustable driving structure comprises a moving plate (18), wherein the outer sides of a first permanent magnet (6) and a second permanent magnet (13) are fixedly connected with a first hinge shaft (16) and a second hinge shaft (17), the moving plate (18) is positioned between the first permanent magnet (6) and the second permanent magnet (13), connecting plates (19) are respectively arranged at the front and the rear of the moving plate (18), one ends of two groups of connecting plates (19) close to the first permanent magnet (6) are respectively hinged with the first hinge shaft (16), one ends of two groups of connecting plates (19) close to the second permanent magnet (13) are respectively provided with an opening (20), the openings (20) are in sliding connection with the second hinge shaft (17), the lower part of the moving plate (18) is provided with a sliding groove (21), a plurality of groups of sliding blocks (22) are in equidistant sliding connection in the sliding groove (21), the lower parts of the sliding blocks (22) are respectively hinged with a third piston rod (23), one ends of the third piston rods (23) far away from the moving plate (18) penetrate through a shell (7) and are respectively positioned in groups of moving cylinders (10), and the third piston rods (24) are respectively connected with the third piston rods (24) in a sliding mode, and the inner parts of the third piston rods (24) are respectively connected with the sliding cylinders in a sliding mode;
A first adjusting plate (25) and a second adjusting plate (26) which are vertically arranged are respectively arranged on two sides of the interior of the shell (7), and a plurality of groups of pin holes (27) which are vertically distributed are respectively arranged on the first adjusting plate (25) and the second adjusting plate (26);
One side that casing (7) is close to bracing piece (4) is equipped with first fluting (28), second fluting (29) respectively, first fluting (28), second fluting (29) respectively with first regulating plate (25), second regulating plate (26) are corresponding, first fluting (28), second fluting (29) are connected with first round pin pole (30), second round pin pole (31) respectively in the sliding, the one end of first round pin pole (30) passes pinhole (27) and contacts and magnetic adsorption with first permanent magnet (6) upper surface, the one end of second round pin pole (31) passes pinhole (27) and contacts with second permanent magnet (13) upper surface.
2. The full-automatic blood coagulation and blood coagulation inhibition test pipetting device according to claim 1, wherein liquid taking heads (32) are arranged at the lower ends of a plurality of groups of pipetting cylinders (10), suction heads (33) are detachably connected to the lower ends of the liquid taking heads (32), squeeze plates (34) are slidably connected to the outer sides of the liquid taking heads (32), a plurality of groups of holes are formed in the squeeze plates (34), the holes are slidably connected with the liquid taking heads (32), and an extrusion structure is arranged below the shell (7) and fixedly connected with the squeeze plates (34).
3. The full-automatic blood coagulation and blood coagulation inhibition test pipetting device according to claim 2, wherein the extrusion structure comprises two groups of fixed cylinders (35) arranged on two sides of the lower surface of the shell (7), electromagnets (36) are arranged in the two groups of fixed cylinders (35), the upper surfaces of the two groups of electromagnets (36) respectively correspond to the lower surfaces of the first permanent magnet (6) and the second permanent magnet (13), driving rods (37) are fixedly connected to the lower surfaces of the two groups of electromagnets (36), and one ends of the driving rods (37) far away from the electromagnets (36) respectively penetrate through the fixed cylinders (35) and are in sliding connection with the fixed cylinders (35), and the lower ends of the driving rods (37) are respectively fixedly connected with the upper surface of the extrusion plate (34);
the fixed cylinder (35) is internally provided with a return spring (38), one end of the return spring (38) is fixedly connected with the lower surface of the electromagnet (36), and the other end of the return spring (38) is fixedly connected with the inner bottom of the fixed cylinder (35).
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| CN202310983178.1A CN116908479B (en) | 2023-08-07 | 2023-08-07 | Full-automatic hemagglutination and hemagglutination inhibition test pipetting device |
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| CN202310983178.1A CN116908479B (en) | 2023-08-07 | 2023-08-07 | Full-automatic hemagglutination and hemagglutination inhibition test pipetting device |
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| CN117405675B (en) * | 2023-12-14 | 2024-02-13 | 胜佳明(山东)智能科技有限公司 | Imaging device for hemagglutination inhibition test workstation |
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