CN116903695A - Cyclic peptide compound, preparation method thereof, pharmaceutical composition containing cyclic peptide compound and application of cyclic peptide compound - Google Patents

Abstract

The invention provides a cyclopeptide compound, a preparation method thereof, a pharmaceutical composition containing the cyclopeptide compound and application thereof, in particular to a cyclopeptide compound with a structure shown in a formula I, which has the function of remarkably inhibiting main protease (M ^pro Or 3CL ^pro ) Is expected to be used for preparing medicines for treating diseases caused by coronavirus infection including SARS-CoV-2, SARS-CoV-1, MERS-CoV, HCoV, TGEV, MHV, BCoV, IBV and the like.

Description

Cyclic peptide compound, preparation method thereof, pharmaceutical composition containing cyclic peptide compound and application of cyclic peptide compound

Technical Field

The invention belongs to the field of pharmaceutical chemistry, and in particular relates to a cyclopeptide compound, a preparation method thereof, a pharmaceutical composition containing the cyclopeptide compound and application of the cyclopeptide compound.

Background

New coronapneumonia is a disease caused by infection with SARS-CoV-2 virus. SARS-CoV-2 virus is a single-stranded RNA virus that is constantly mutated, resulting in reduced effectiveness of vaccines and neutralizing antibodies. After invasion of the host cell by the SARS-CoV-2 virus, two folded polyproteins are translated. These two multimeric proteins consist essentially of a 33.8-k dalton virus-specific main protease (M ^pro Also known as 3C-like protease, 3CL ^pro ) And the hydrolysis treatment provides proteins with different structures and functions, so that the purpose of virus replication is achieved. M is M ^pro Highly conserved among coronaviruses such as SARS-CoV-1, MERS-CoV and SARS-CoV-2 (Ullrich S, nitsche C. The SARS-CoV-2main protease as drug target.Bioorg.Med.Chem.Lett.2020;30 (17): 127377.) is expected to develop broad-spectrum anti-coronavirus drugs. Before SARS-CoV-2 occurs, M ^pro Are considered potential targets against SARS-CoV-1 and MERS-CoV viruses. However, due to the previous M ^pro The PK inhibitors are very poor and can only be administered by intravenous injection clinically, limiting the clinical utility of these compounds. At month 4 of 2021, M was first orally absorbed by the development of pyroxene ^pro The inhibitor PF-07321332 entered clinical trials. However, PF-07321332 has low bioavailability and requires combination with the CYP3A4 inhibitor ritonavir (NCT 04960202).

Therefore, there is an urgent need in the art to develop more efficient and more pharmaceutically acceptable M ^pro An inhibitor.

Disclosure of Invention

The invention aims to provide an M with higher efficiency and better pharmacy ^pro An inhibitor.

In a first aspect of the present invention, there is provided a cyclic peptide compound of formula I, or a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuteride or combination thereof,

Wherein,,

a is selected from the sequences:

wherein the N atom in the sequence is connected with a carbonyl group, and the carbonyl group in the sequence is connected with the N atom;

R ² selected from: H. C1-C6 alkyl, C3-C8 cycloalkyl, C2-C6 alkenyl, C4-C6 cycloalkenyl, C3-C8 cycloalkyl-CH ₂ -and C4-C6 cycloalkenyl-CH ₂ -said alkyl, alkenyl, cycloalkyl, cycloalkenyl optionally substituted by halogen, C1-C3 alkyl, C1-C3 haloalkyl or C2-C4 alkenyl;

R ³ selected from: H. C1-C4 alkyl and C1-C4 haloalkyl;

ring B is an optionally substituted 3-12 membered heterocycle; wherein the substituents are selected from: C1-C3 alkyl, C1-C3 haloalkyl or halogen;

R ¹ selected from: C1-C6 alkyl, C3-C8 cycloalkyl, C6-C10 aryl, 5-to 10-membered heteroaryl, C1-C6 alkyl-CO-, C3-C8 cycloalkyl-CO-, 3-to 12-membered heterocyclyl-CO-, C6-C10 aryl-CO-, 5-to 10-membered heteroaryl-CO-, C1-C4 alkyloxy-CO-, C1-C4 alkylamino-CO-, C1-C6 alkyl-SO ₂ -, a part of C3-C8 cycloalkyl-SO ₂ -and 3-12 membered heterocyclyl-SO ₂ -; wherein the alkyl, cycloalkyl, heterocyclyl, aryl and heteroaryl groups may be optionally substituted with halogen, C1-C3 alkyl, C1-C3 alkoxy, formylamino, C1-C3 alkylamino, C3-C8 cycloalkyl, 3-12 membered heterocyclyl, C6-C10 aryl, or 5-10 membered heteroaryl; wherein said alkyl, formylamino, alkylamino, cycloalkyl, heterocyclyl, aryl and heteroaryl groups are optionally substituted with halogen, C1-C3 alkyl, C1-C3 alkoxy, hydroxy, amino, (C1-C3 alkyl) ₂ Amino, C1-C3 haloalkoxy, C3-C8 cycloalkylA group, a C3-C8 halocycloalkyl, a 3-12 membered heterocyclyl, a 3-12 membered haloheterocyclyl, a C6-C10 aryl, or a 5-10 membered heteroaryl substitution;

L ¹ selected from: -CO-and-CR ^a R ^b -; and when L ¹ In the case of-CO-, L ² is-NR ^c -, or-O-, L ³ is-CR ^a R ^b -; when L ¹ is-CR ^a R ^b -when, L ² is-NR ^c -, -O-or-CR ^a R ^b -，L ³ Is absent, -CO-or-CR ^a R ^b -；

Each R is ^a Independently selected from: absence, H, halogen, -OH, oxo, mercapto, cyano, -NH ₂ Optionally substituted C1-C6 alkyl, optionally substituted C1-C6 alkoxy, optionally substituted 3-8 membered cycloalkyl or optionally substituted 4-8 membered heterocyclyl, or R ^a And R is R ^b Is linked to C to form an optionally substituted 3-8 membered carbocyclic ring or 4-8 membered heterocyclic ring; wherein the heterocycle comprises 1-3 heteroatoms selected from N, O, S, P; wherein the substituted substituent is R;

each R is ^b Independently selected from: absence, H, halogen, -OH, oxo, mercapto, cyano, -NH ₂ Optionally substituted C1-C6 alkyl, optionally substituted C1-C6 alkoxy, optionally substituted 3-8 membered cycloalkyl or optionally substituted 4-8 membered heterocyclyl, or R ^a And R is R ^b Is linked to C to form an optionally substituted 3-8 membered carbocyclic ring or 4-8 membered heterocyclic ring; wherein the heterocycle comprises 1-3 heteroatoms selected from N, O, S, P; wherein the substituted substituent is R;

Each R is independently selected from: halogen, -OH, oxo (= O), -NH ₂ C3-C8 cycloalkyl, 3-8 membered heterocyclyl, C1-C4 alkyl or C1-C4 haloalkyl-;

R ^c selected from: h or C1-C3 alkyl;

n is 2, 3 or 4.

In a further preferred embodiment of the present invention,selected from:

In another preferred embodiment, a is selected from the sequences:

the N atoms in the sequence are connected with carbonyl groups, and the carbonyl groups in the sequence are connected with the N atoms;

R ³ selected from: H. C1-C4 alkyl or C1-C4 haloalkyl.

In another preferred embodiment, R ¹ The ring is selected from any one of the following groups:

in another preferred embodiment, each R ^a Selected from: H. halogen or methyl.

In another preferred embodiment, each R ^b Selected from: H. halogen or methyl.

In another preferred embodiment, the ring A, B, R ¹ 、R ² 、R ³ 、R ^a 、R ^b And n is a group corresponding to each specific compound in the examples.

In another preferred embodiment, the compound is selected from the group consisting of:

in a second aspect of the invention, there is provided a compound of formula II, or a salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph or deuterate thereof,

wherein,,

R ⁴ is that

R ⁷ And R is ⁸ Each independently selected from: H. C1-C6 alkyl, C2-C4 alkenyl, C1-C6 alkyl-CO-, C3-C8 cycloalkyl-CO-, C1-C6 alkyl-SO ₂ -, C1-C6 alkyl-SO-; 5-10 membered aryl-CO-, 5-10 membered aryl-SO ₂ -, 5-12 membered heteroaryl-CO-, 5-12 membered heteroaryl-SO ₂ -, C1-C6 alkyl-O-CO-; 5-10 membered aryl-CH ₂ -O-CO-or 5-12 membered heteroaryl-CH ₂ -O-CO-, said alkyl, alkenyl, cycloalkyl, aryl and heteroaryl groups optionally being substituted with one or more substituents selected from the group consisting of: halogen, C1-C3 alkyl, C3-C8 cycloalkyl, C1-C3 alkoxy or phenyl, wherein said phenyl is optionally substituted with one or more substituents selected from the group consisting of: halogen, methyl or methoxy; or R is ⁷ And R is R ⁸ And together with the N atoms to which they are attached form a 4-8 membered heterocyclic ring optionally substituted with a substituent of group X; wherein the heterocyclic ring comprises 0-2 heteroatoms selected from N, O, S or P in addition to the attached N atom;

R ⁹ and R is ¹⁰ Each independently selected from: a C1-C6 alkyl, 5-10 membered aryl or 5-12 membered heteroaryl, said alkyl, aryl, heteroaryl optionally substituted with a substituent of group X;

R ⁵ is that

R ¹¹ Selected from: H. C1-C6 alkyl, C2-C4 alkenyl, C3-C6 cycloalkyl, benzyl orThe alkyl, alkenyl, cycloalkyl and benzyl groups may be optionally substituted with one or more substituents selected from the group consisting of: halogen, methyl or methoxy;

R ^11a 、R ^11b And R is ^11c Each independently selected from: C1-C4 alkyl or phenyl;

R ¹² and R is ¹³ Each independently selected from: H. C1-C6 alkyl, C2-C4 alkenyl, C1-C6 alkyl-CO-, C3-C8 cycloalkyl-CO-, C1-C6 alkyl-SO ₂ -, C1-C6 alkyl-SO-; 5-10 membered aryl-CO-, 5-10 membered aryl-SO ₂ -, 5-12 membered heteroaryl-CO-, 5-12 membered heteroaryl-SO ₂ -, C1-C6 alkyl-O-CO-; 5-10 membered aryl-CH ₂ -O-CO-, 5-12 membered heteroaryl-CH ₂ -O-CO-or C2-C4 alkenyl-O-CO-, said alkyl, alkenyl, cycloalkyl, aryl and heteroaryl groups optionally being substituted with one or more substituents selected from the group consisting of: halogen, C1-C3 alkyl, C3-C8 cycloalkyl, C1-C3 alkoxy or phenyl, said phenyl optionally being substituted with one or more substituents selected from the group consisting of: halogen, methyl and methoxy; or R is ¹² And R is R ¹³ And together with the N atoms to which they are attached form a 4-8 membered heterocyclic ring optionally substituted with a substituent of group X; wherein the heterocyclic ring comprises 0-2 heteroatoms selected from N, O, S or P in addition to the attached N atom;

the substituents of group X are selected from: oxo, halogen, C1-C6 alkyl or C1-C6 alkoxy;

R ⁶ selected from: H. C1-C6 alkyl, C2-C4 alkenyl, C1-C6 alkyl-SO ₂ -, 5-to 10-membered aryl-SO ₂ -, C1-C6 alkyl-O-CO-; C2-C4 alkenyl-O-CO-, 5-10 membered aryl-CH ₂ -O-CO-or 5-12 membered heteroaryl-CH ₂ -O-CO-, said alkyl, alkenyl, cycloalkyl, aryl and heteroaryl groups optionally being substituted with one or more substituents selected from the group consisting of:halogen, C1-C3 alkyl, C3-C8 cycloalkyl, C1-C3 alkoxy or phenyl, said alkoxy and phenyl optionally being substituted with one or more substituents selected from the group consisting of: halogen, methyl, methoxy, nitro or trimethylsilyl.

In another preferred embodiment, R ⁴ Selected from: amino or amino groups

In another preferred embodiment, R ⁵ Selected from:

in another preferred embodiment, R ⁶ Selected from: boc, allyl, benzyl or p-methoxybenzyl.

In a third aspect of the invention, there is provided a pharmaceutical composition comprising a compound of the first aspect and a pharmaceutically acceptable carrier.

Preferably, the pharmaceutical composition further comprises other pharmaceutically acceptable therapeutic agents, in particular other anti-coronavirus drugs. Such therapeutic agents include, but are not limited to: RNA dependent RNA polymerase inhibitors such as Ruidexivir, molnupiravir and the like, and cytochrome oxidase inhibitors such as ritonavir and the like.

In a fourth aspect of the present invention, there is provided a use of a compound according to the first aspect or a pharmaceutical composition according to the third aspect, selected from any one of the following (a) to (c):

(a) Preparation of a protease (M) useful for prophylaxis and/or treatment and a primary protease (M) ^pro ) Or 3C-like protease (3 CL) ^pro ) Medicaments for related diseases associated with viral infections;

(b) Preparation of the protease (M) for in vitro non-therapeutic and Main protease(s) ^pro ) Or 3C-like protease (3 CL) ^pro ) An inhibitor related to the activity of (a); or (b)

(c) Preparation of proliferation inhibitors for in vitro non-therapeutic coronaviruses.

In another preferred embodiment, the disease is selected from: respiratory tract infections, pneumonia, complications thereof, or combinations thereof.

In a fifth aspect of the present invention, there is provided a method for inhibiting a main protease (M ^pro ) Or 3C-like protease (3 CL) ^pro ) A method of activity comprising the steps of: the compound of the first aspect, or a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuterate, or combination thereof, is combined with a primary protease (M ^pro ) Or 3C-like protease (3 CL) ^pro ) Contacting, thereby inhibiting the main protease (M ^pro ) Or 3C-like protease (3 CL) ^pro ) Preferably, the method is non-therapeutic and non-diagnostic, more preferably, the method is in vitro.

In another preferred embodiment, the main protease (M ^pro ) Or 3C-like protease (3 CL) ^pro ) Is recombinant or expressed by coronavirus.

In a preferred embodiment, the protease (M ^pro ) Or 3C-like protease (3 CL) ^pro ) The viruses concerned are selected from the group consisting of: covd-19, SARS, MERS, etc. The viruses include wild-type and mutant strains.

Detailed Description

The present inventors have studied extensively and intensively, and have unexpectedly developed a cyclic peptide compound which can effectively inhibit the activity of a main protease (Mpro) or a 3C-like protease (3 CLpro) for the first time, and which can be used for the preparation of a medicament for the treatment and/or prevention of diseases caused by coronavirus infection including SARS-CoV-2, SARS-CoV-1, MERS-CoV, HCoV, TGEV, MHV, BCoV, IBV, etc. On this basis, the present invention has been completed.

Description of the terms

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

As used herein, the term "comprising" or "including" can be open, semi-closed, and closed. In other words, the term also includes "consisting essentially of …," or "consisting of ….

The invention will be further illustrated with reference to specific examples. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. The experimental methods, in which specific conditions are not noted in the following examples, are generally conducted under conventional conditions or under conditions recommended by the manufacturer. Percentages and parts are by weight unless otherwise indicated.

Definition of groups

The definition of standard chemical terms can be found in references (including Carey and Sundberg "ADVANCED ORGANIC CHEMISTRY TH ED." vols. A (2000) and B (2001), plenum Press, new York). Conventional methods within the skill of the art, such as mass spectrometry, NMR, IR and UV/VIS spectroscopy, and pharmacological methods are employed unless otherwise indicated. Unless specifically defined otherwise, the terms used herein in the description of analytical chemistry, organic synthetic chemistry, and pharmaceutical chemistry are known in the art. Standard techniques may be used in chemical synthesis, chemical analysis, pharmaceutical preparation, formulation and delivery, and treatment of patients. For example, the reaction and purification can be carried out using the manufacturer's instructions for the kit, or in a manner well known in the art or in accordance with the teachings of the present invention. The techniques and methods described above may generally be practiced according to conventional methods well known in the art, based on a number of general and more specific descriptions in the literature cited and discussed in this specification. In this specification, groups and substituents thereof can be selected by one skilled in the art to provide stable moieties and compounds.

When substituents are described by conventional formulas written from left to right, the substituents also include chemically equivalent substituents obtained when writing formulas from right to left. For example, -CH ₂ O-is equivalent to-OCH ₂ -。

The section headings used herein are for purposes of organizing articles only and should not be construed as limiting the subject matter. All documents or portions of documents cited in this disclosure, including but not limited to patents, patent applications, articles, books, operating manuals, and treatises, are hereby incorporated by reference in their entirety.

Certain chemical groups defined herein are preceded by a simplified symbol to indicate the total number of carbon atoms present in the group. For example, C1-C6 alkyl refers to an alkyl group as defined below having a total of 1 to 6 carbon atoms. The total number of carbon atoms in the reduced notation does not include carbon that may be present in a substituent of the group.

In addition to the foregoing, when used in the specification and claims of the present application, the following terms have the meanings indicated below, unless otherwise specified.

In the present application, the term "halogen" refers to fluorine, chlorine, bromine or iodine.

"hydroxy" refers to an-OH group.

"hydroxyalkyl" refers to an alkyl group as defined below substituted with a hydroxyl (-OH).

"carbonyl" refers to a-C (=o) -group.

"nitro" means-NO ₂ 。

"cyano" refers to-CN.

"amino" means-NH ₂ 。

"substituted amino" refers to an amino group substituted with one or two alkyl, alkylcarbonyl, arylalkyl, heteroarylalkyl groups as defined below, e.g., mono-alkylamino, di-alkylamino, alkylamido, arylalkylamino, heteroarylalkylamino.

"carboxy" refers to-COOH.

In the present application, as part of a group or other group (e.g., as used in halogen substituted alkyl groups and the like), the term "alkyl" refers to a straight or branched hydrocarbon chain radical that is fully saturated, consisting of only carbon and hydrogen atoms, having, for example, from 1 to 12 (preferably from 1 to 8, more preferably from 1 to 6) carbon atoms, and being attached to the remainder of the molecule by 1 or more single bonds, including, for example, but not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, 2-methylbutyl, 2-dimethylpropyl, n-hexyl, heptyl, 2-methylhexyl, 3-methylhexyl, octyl, nonyl, decyl, and the like. For the purposes of the present application, the term "alkyl" preferably denotes an alkyl group containing from 1 to 6 carbon atoms.

In the present application, the term "alkenyl" as part of a group or other group means a straight or branched hydrocarbon chain group consisting of only carbon and hydrogen atoms, containing at least one double bond, having, for example, 2 to 14 (preferably 2 to 10, more preferably 2 to 6) carbon atoms, and being linked to the rest of the molecule by 1 or more single bonds, such as, but not limited to, vinyl, propenyl, allyl, but-1-enyl, but-2-enyl, pent-1, 4-dienyl, and the like.

The term "alkynyl" as part of a group or other group herein means a straight or branched hydrocarbon chain group consisting of only carbon and hydrogen atoms, containing at least one carbon-carbon triple bond, having, for example, 2 to 14 (preferably 2 to 10, more preferably 2 to 6) carbon atoms, and being attached to the remainder of the molecule by 1 or more single bonds, such as, but not limited to, ethynyl, 1-propynyl, 1-butynyl, heptynyl, octynyl, and the like.

In the present application, as part of a group or other group, the term "carbocycle (group)" means a stable, non-aromatic, mono-or polycyclic hydrocarbon group consisting of only carbon atoms and hydrogen atoms, which may include fused ring systems, bridged ring systems, or spiro ring systems, having 3 to 15 carbon atoms, preferably having 3 to 10 carbon atoms, more preferably having 3 to 8 carbon atoms, more preferably 3 to 6 carbon atoms, and which is a saturated or unsaturated ring (i.e., cycloalkyl, cycloalkenyl, etc.) and may be attached to the remainder of the molecule by 1 or more single bonds via any suitable carbon atom. Unless otherwise specifically indicated in the present specification, carbon atoms in a carbocyclyl group may optionally be oxidized. Examples of carbocyclyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, adamantyl, 2, 3-indanyl, octahydro-4, 7-methylene-1H-indenyl, 1,2,3, 4-tetrahydro-naphthyl, 5,6,7, 8-tetrahydro-naphthyl, cyclopentenyl, cyclohexenyl, cyclohexadienyl, 1H-indenyl, 8, 9-dihydro-7H-benzocyclohepten-6-yl, 6,7,8, 9-tetrahydro-5H-benzocycloheptenyl, 5,6,7,8,9, 10-hexahydro-benzocyclooctenyl, fluorenyl, bicyclo [1.1.1] pentane, bicyclo [2.2.1] heptyl, 7-dimethyl-bicyclo [2.2.1] heptyl, bicyclo [2.2.1] heptenyl, bicyclo [ 2.2.2.2 ] octyl, bicyclo [3.1 ] cycloheptyl, bicyclo [1.1.1] octanyl, bicyclo [ 2.2.1.1 ] octanyl, and the like.

In the present application, the term "cycloalkyl" as part of a group or other group refers to the fully saturated carbocyclic ring (group) described above, typical cycloalkyl groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, adamantyl, and the like.

In the present application, as part of a group or other group, the term "cycloalkenyl" refers to a partially unsaturated carbocyclic ring (group), typical cycloalkenyl groups include, but are not limited to, cyclobutenyl, cyclopentenyl, cyclohexenyl, and the like.

In the present application, the term "heterocyclic (group)" means a stable 3-to 20-membered non-aromatic cyclic group consisting of 2 to 14 carbon atoms and 1 to 6 hetero atoms selected from nitrogen, phosphorus, oxygen and sulfur as part of a group or other groups. Unless specifically indicated otherwise in the present specification, a heterocyclyl group may be a monocyclic, bicyclic, tricyclic or more cyclic ring system, which may include fused, bridged or spiro ring systems; the nitrogen, carbon or sulfur atoms in the heterocyclyl may optionally be oxidized; the nitrogen atom may optionally be quaternized; and the heterocyclyl may be partially or fully saturated. The heterocyclic group may be attached to the remainder of the molecule via a carbon atom or heteroatom and through 1 or more single bonds. In heterocyclyl groups containing fused rings, one or more of the rings may be aryl or heteroaryl as defined below, provided that the point of attachment to the remainder of the molecule is a non-aromatic ring atom. For the purposes of the present application, heterocyclyl groups are preferably stable 4-to 11-membered non-aromatic monocyclic, bicyclic, bridged or spiro groups comprising 1 to 3 heteroatoms selected from nitrogen, oxygen and sulfur, more preferably stable 4-to 8-membered non-aromatic monocyclic, bicyclic, bridged or spiro groups comprising 1 to 3 heteroatoms selected from nitrogen, oxygen and sulfur. Examples of heterocyclyl groups include, but are not limited to: pyrrolidinyl, morpholinyl, piperazinyl, homopiperazinyl, piperidinyl, thiomorpholinyl, 2-azabicyclo [2.2.2] octanyl, 2, 7-diaza-spiro [3.5] nonan-7-yl, 2-oxa-6-aza-spiro [3.3] heptan-6-yl, 2, 5-diaza-bicyclo [2.2.1] heptan-2-yl, azetidinyl, pyranyl, tetrahydropyranyl, thiopyranyl, tetrahydrofuranyl, oxazinyl, dioxacyclopentyl, tetrahydroisoquinolyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, quinozinyl, thiazolidinyl, isothiazolidinyl, isoxazolidinyl, indolinyl, octahydroindolyl, octahydroisoindolyl, pyrrolidinyl, pyrazolidinyl, phthalimidyl, and the like.

In the present application, the term "aryl" as part of a group or other group means a conjugated hydrocarbon ring system group having 6 to 18 carbon atoms, preferably having 6 to 10 carbon atoms. For the purposes of the present application, aryl groups may be monocyclic, bicyclic, tricyclic or more ring systems, and may also be fused to a carbocyclyl or heterocyclyl group as defined above, provided that the aryl groups are linked to the remainder of the molecule via 1 or more single bonds via atoms on the aromatic ring. Examples of aryl groups include, but are not limited to, phenyl, naphthyl, anthryl, phenanthryl, fluorenyl, 2, 3-dihydro-1H-isoindolyl, 2-benzoxazolinone, 2H-1, 4-benzoxazin-3 (4H) -one-7-yl, and the like.

In the present application, the term "arylalkyl" refers to an alkyl group as defined above substituted with an aryl group as defined above.

In the present application, the term "heteroaryl" as part of a group or other group means a 5-to 16-membered conjugated ring system group having 1 to 15 carbon atoms (preferably 1 to 10 carbon atoms) and 1 to 6 heteroatoms selected from nitrogen, oxygen and sulfur in the ring. Unless otherwise specifically indicated in the present specification, heteroaryl groups may be monocyclic, bicyclic, tricyclic or more ring systems, and may also be fused to a carbocyclyl or heterocyclyl group as defined above, provided that the heteroaryl groups are attached to the remainder of the molecule via 1 or more single bonds via an atom on a heteroaromatic ring. The nitrogen, carbon, or sulfur atoms in the heteroaryl group may optionally be oxidized; the nitrogen atom may optionally be quaternized. For the purposes of the present application, heteroaryl groups are preferably stable 5-to 12-membered aromatic groups comprising 1 to 5 heteroatoms selected from nitrogen, oxygen and sulfur, more preferably stable 5-to 10-membered aromatic groups comprising 1 to 4 heteroatoms selected from nitrogen, oxygen and sulfur or 5-to 6-membered aromatic groups comprising 1 to 3 heteroatoms selected from nitrogen, oxygen and sulfur. Examples of heteroaryl groups include, but are not limited to, thienyl, imidazolyl, pyrazolyl, thiazolyl, oxazolyl, oxadiazolyl, isoxazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, benzimidazolyl, benzopyrazolyl, indolyl, furanyl, pyrrolyl, triazolyl, tetrazolyl, triazinyl, indolizinyl, isoindolyl, indazolyl, isoindazolyl, purinyl, quinolinyl, isoquinolinyl, naphthyridinyl, quinoxalinyl, pteridinyl, carbazolyl, carbolinyl, phenanthridinyl, phenanthrolinyl, acridinyl, phenazinyl, isothiazolyl, benzothiazolyl, benzothienyl, oxatriazolyl, cinnolinyl, quinazolinyl, thiophenyl, indolizinyl, phenanthroline, isoxazolyl, phenoxazinyl, phenothiazinyl, 4,5,6, 7-tetrahydrobenzo [ b ] thienyl, naphthyridinyl, [1,2,4] triazolo [4, 3-triazolo [1, 4] pyridazine, 3-1, 4-imidazo [1, 4] triazolo [1, 4, 3-triazolo [1, 4] pyridazine, 3-1, 4-imidazo [ 2,4] a ] 1, 4-imidazo [ 2, 4-a ] and the like.

In the present application, the term "heteroarylalkyl" refers to an alkyl group as defined above substituted with a heteroaryl group as defined above.

In the present application, the term "absent" means that both sides of the groups defined above are directly connected by chemical bonds. For example, the absence of B in "A-B-C" means "A-C".

In the present application,is->Represents the attachment position of the group R.

In the present application, the removal of"optionally" and "optionally" in the claims means that the subsequently described event or condition may or may not occur, and that the description includes instances where the event or condition occurs and does not occur. For example, "optionally substituted aryl" means that a hydrogen on the aryl is substituted or unsubstituted, and the description includes both substituted and unsubstituted aryl. For example, where substituents are not explicitly listed, the term "optionally substituted", "substituted" or "substituted with … …" as used herein means that one or more hydrogen atoms on a given atom or group are independently substituted with one or more, e.g., 1, 2, 3, or 4 substituents independently selected from: deuterium (D), halogen, -OH, -oxo (=o), mercapto, cyano, -CD ₃ -C1-C6 alkyl (preferably-C1-3 alkyl), C2-C6 alkenyl, C2-C6 alkynyl, cycloalkyl (preferably-C3-C8 cycloalkyl), aryl, heterocyclyl (preferably 3-8 membered heterocyclyl), heteroaryl, aryl-C1-C6 alkyl-, heteroaryl-C1-C6 alkyl-, C1-C6 haloalkyl-, -OC1-C6 alkyl (preferably-OC 1-C3 alkyl), -OC2-C6 alkenyl, -Ocycloalkyl, -Oheterocyclyl, -Oaryl, -Oheteroaryl, -OC1-C6 alkylphenyl, -C1-C6 alkyl-OH (preferably-C1-C4 alkyl-OH), -C1-C6 alkyl-SH, -C1-C6 alkyl-O-C1-C6 alkyl, -OC1-C6 haloalkyl, -NH ₂ -C1-C6 alkyl-NH ₂ (preferably-C1-C3 alkyl-NH) ₂ ) -N (C1-C6 alkyl) ₂ (preferably-N (C1-C3 alkyl) ₂ ) -NH (C1-C6 alkyl) (preferably-NH (C1-C3 alkyl)), -N (C1-C6 alkyl) (C1-C6 alkylphenyl), -NH (C1-C6 alkylphenyl), -N (C1-C6 alkyl) (aryl), -NH (aryl), nitro, -C (O) -OH, -C (O) OC1-C6 alkyl (preferably-C (O) OC1-C3 alkyl), -CONR ⁱ R ⁱⁱ (wherein R is ⁱ And R is ⁱⁱ Is H, D and C1-6 alkyl, preferably C1-3 alkyl), -NHC (O) (C1-C6 alkyl), -NHC (O) (phenyl), -N (C1-C6 alkyl) C (O) (C1-C6 alkyl), -N (C1-C6 alkyl) C (O) (phenyl), -C (O) C1-C6 alkyl, -C (O) heteroaryl (preferably-C (O) -5-7 membered heteroaryl), -C (O) C1-C6 alkylphenyl, -C (O) C1-C6 haloalkyl, -OC (O) C1-C6 alkyl (preferably-OC (O) C1-C3 alkyl), -S (O) ₂ -C1-C6 alkyl, -S (O) ₂ -phenyl, -S (O) ₂ C1-C6 haloalkyl-S(O) ₂ NH ₂ 、-S(O) ₂ NH (C1-C6 alkyl), -S (O) ₂ NH (phenyl), -NHS (O) ₂ (C1-C6 alkyl), -NHS (O) ₂ (phenyl) and-NHS (O) ₂ (C1-C6 haloalkyl), wherein each of said alkyl, alkenyl, alkynyl, cycloalkyl, phenyl, aryl, heterocyclyl, and heteroaryl is optionally further substituted with one or more substituents selected from the group consisting of: halogen, -OH, oxo (= O), -NH ₂ Cycloalkyl, 3-8 membered heterocyclyl, C1-C4 alkyl, C1-C4 haloalkyl-, -OC1-C4 alkyl, -C1-C4 alkyl-OH, -C1-C4 alkyl-O-C1-C4 alkyl, -OC1-C4 haloalkyl, cyano, nitro, -C (O) -OH, -C (O) OC1-C6 alkyl, -CON (C1-C6 alkyl) ₂ -CONH (C1-C6 alkyl), -CONH ₂ -NHC (O) (C1-C6 alkyl), -NH (C1-C6 alkyl) C (O) (C1-C6 alkyl), -SO ₂ (C1-C6 alkyl), -SO ₂ (phenyl) -SO ₂ (C1-C6 haloalkyl), -SO ₂ NH ₂ 、-SO ₂ NH (C1-C6 alkyl), -SO ₂ NH (phenyl) -NHSO ₂ (C1-C6 alkyl), -NHSO ₂ (phenyl) and-NHSO ₂ (C1-C6 haloalkyl). When an atom or group is substituted with multiple substituents, the substituents may be the same or different. The terms "moiety", "structural moiety", "chemical moiety", "group", "chemical group" as used herein refer to a particular fragment or functional group in a molecule. Chemical moieties are generally considered to be chemical entities that are embedded or attached to a molecule.

In the present invention, (C1-C4 alkyl) ₂ Amino, representing 2C 1-C4 alkyl-substituted amines, may be, for example Etc.

In the present invention, "a plurality" means 2, 3 or 4.

Active ingredient

As used herein, "inventive compound" or "active ingredient" refers to a compound represented by formula I, and further comprises a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuterate, or combination thereof.

"stereoisomers" refer to compounds that consist of the same atoms, are bonded by the same bonds, but have different three-dimensional structures. The present invention is intended to cover various stereoisomers and mixtures thereof.

When an olefinic double bond is contained in the compounds of the present invention, the compounds of the present invention are intended to include both E-and Z-geometric isomers unless otherwise specified.

"tautomer" refers to an isomer formed by the transfer of a proton from one atom of a molecule to another atom of the same molecule. All tautomeric forms of the compounds of the invention are also intended to be included within the scope of the invention.

The compounds of the invention or pharmaceutically acceptable salts thereof may contain one or more chiral carbon atoms and thus may be produced in enantiomers, diastereomers and other stereoisomeric forms. Each chiral carbon atom may be defined as (R) -or (S) -, based on stereochemistry. The present invention is intended to include all possible isomers, as well as racemates and optically pure forms thereof. The compounds of the invention may be prepared by selecting racemates, diastereomers or enantiomers as starting materials or intermediates. Optically active isomers may be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques, such as crystallization and chiral chromatography.

Conventional techniques for preparing/separating individual isomers include chiral synthesis from suitable optically pure precursors, or resolution of racemates (or racemates of salts or derivatives) using, for example, chiral high performance liquid chromatography, see, for example, gerald gabiz and Martin g.schmid (eds.), chiral Separations, methods and Protocols, methods in Molecular Biology, vol.243,2004; m.stalcup, chiral Separations, annu.rev.animal.chem.3:341-63, 2010; fumigs et al (EDs.), VOGEL' S ENCYCLOPEDIA OF PRACTICAL ORGANIC CHEMISTRY.sup.TH ED, longman Scientific and Technical Ltd., essex,1991,809-816; heller, acc.chem.Res.1990,23,128.

In the present application, the term "pharmaceutically acceptable salt" includes pharmaceutically acceptable acid addition salts and pharmaceutically acceptable base addition salts.

By "pharmaceutically acceptable acid addition salt" is meant a salt with an inorganic or organic acid that retains the biological effectiveness of the free base without other side effects. Inorganic acid salts include, but are not limited to, hydrochloride, hydrobromide, sulfate, nitrate, phosphate, and the like; organic acid salts include, but are not limited to, formate, acetate, 2-dichloroacetate, trifluoroacetate, propionate, hexanoate, octanoate, decanoate, undecylenate, glycolate, gluconate, lactate, sebacate, adipate, glutarate, malonate, oxalate, maleate, succinate, fumarate, tartrate, citrate, palmitate, stearate, oleate, cinnamate, laurate, malate, glutamate, pyroglutamate, aspartate, benzoate, methanesulfonate, benzenesulfonate, p-toluenesulfonate, alginate, ascorbate, salicylate, 4-aminosalicylate, naphthalenedisulfonate, and the like. These salts can be prepared by methods known in the art.

By "pharmaceutically acceptable base addition salt" is meant a salt formed with an inorganic or organic base that is capable of maintaining the bioavailability of the free acid without other side effects. Salts derived from inorganic bases include, but are not limited to, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum, and the like. Preferred inorganic salts are ammonium, sodium, potassium, calcium and magnesium salts. Salts derived from organic bases include, but are not limited to, the following: primary, secondary and tertiary amines, substituted amines including natural substituted amines, cyclic amines and basic ion exchange resins such as ammonia, isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, diethanolamine, triethanolamine, dimethylethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purine, piperazine, piperidine, N-ethylpiperidine, polyamine resins and the like. Preferred organic bases include isopropylamine, diethylamine, ethanolamine, trimethylamine, dicyclohexylamine, choline, and caffeine. These salts can be prepared by methods known in the art.

Pharmaceutical compositions and methods of administration

In the present application, "pharmaceutical composition" refers to a formulation of a compound of the present application with a medium commonly accepted in the art for delivery of biologically active compounds to a mammal (e.g., a human). The medium includes a pharmaceutically acceptable carrier. The purpose of the pharmaceutical composition is to promote the administration of organisms, facilitate the absorption of active ingredients and further exert biological activity.

The term "pharmaceutically acceptable" as used herein refers to a material (e.g., carrier or diluent) that does not affect the biological activity or properties of the compounds of the present application, and is relatively non-toxic, i.e., the material can be administered to an individual without causing an adverse biological reaction or interacting in an adverse manner with any of the components contained in the composition.

In the present application, "pharmaceutically acceptable carrier" includes, but is not limited to, any adjuvant, carrier, excipient, glidant, sweetener, diluent, preservative, dye/colorant, flavoring agent, surfactant, wetting agent, dispersing agent, suspending agent, stabilizer, isotonizing agent, solvent, or emulsifying agent that is approved by the relevant government regulatory agency as acceptable for human or livestock use.

The "coronavirus-related diseases" of the present application include, but are not limited to, diseases such as new coronavirus (covd-19), severe Acute Respiratory Syndrome (SARS), middle Eastern Respiratory Syndrome (MERS), acute upper respiratory infection, transmissible gastroenteritis of swine, transmissible bronchitis of fowl, murine hepatitis, neonatal calf diarrhea, bovine respiratory infection, etc.

The terms "prevent", "preventing" and "preventing" as used herein include reducing the likelihood of a patient from developing or worsening a disease or condition.

The term "treatment" and other similar synonyms as used herein include the following meanings:

(i) Preventing the occurrence of a disease or disorder in a mammal, particularly when such mammal is susceptible to the disease or disorder, but has not been diagnosed as having the disease or disorder;

(ii) Inhibiting the disease or disorder, i.e., inhibiting its progression;

(iii) Alleviating a disease or condition, i.e., causing the state of the disease or condition to subside; or alternatively

(iv) Alleviating symptoms caused by the disease or condition.

The term "effective amount," "therapeutically effective amount," or "pharmaceutically effective amount" as used herein refers to an amount of at least one agent or compound that is sufficient to alleviate one or more symptoms of the disease or disorder being treated to some extent after administration. The result may be a reduction and/or alleviation of signs, symptoms, or causes of a disease, or any other desired alteration of a biological system. For example, an "effective amount" for treatment is the amount of a composition comprising a compound disclosed herein that is required to provide clinically significant relief from a disorder. Effective amounts suitable in any individual case can be determined using techniques such as a dose escalation test.

The terms "administering," "administering," and the like as used herein refer to a method capable of delivering a compound or composition to a desired site for biological action. These methods include, but are not limited to, oral routes, duodenal routes, parenteral injection (including intravenous, subcutaneous, intraperitoneal, intramuscular, intraarterial injection or infusion), topical administration, and rectal administration. Application techniques useful in the compounds and methods described herein are well known to those skilled in the art, for example, at Goodman and Gilman, the Pharmacological Basis of Therapeutics, current ed.; pergamon; and Remington's, pharmaceutical Sciences (current edition), mack Publishing co., easton, pa. In preferred embodiments, the compounds and compositions discussed herein are administered orally.

The terms "pharmaceutical combination", "co-administration", "administration of other treatments", "administration of other therapeutic agents" and the like as used herein refer to a pharmaceutical treatment obtained by mixing or combining more than one active ingredient, which includes both fixed and non-fixed combinations of active ingredients. The term "fixed combination" refers to the simultaneous administration of at least one compound described herein and at least one synergistic agent to a patient in the form of a single entity or single dosage form. The term "ambulatory combination" refers to the simultaneous administration, co-administration, or sequential administration of at least one compound described herein and at least one synergistic formulation as separate entities to a patient at variable intervals. These also apply to cocktail therapies, for example, administration of three or more active ingredients.

Process for the preparation of compounds

The following schemes describe methods for preparing compounds of formula I and formula II. In some cases, the order of the steps of the reaction scheme may be altered to promote the reaction or to avoid unwanted side reaction products. The compounds of the present invention may also optionally be conveniently prepared by combining the various synthetic methods described in this specification or known in the art, such combination being readily apparent to those skilled in the art to which the present invention pertains.

It will also be appreciated by those skilled in the art that in the methods described below, the intermediate compound functional groups may need to be protected by appropriate protecting groups. Such functional groups include hydroxyl, amino, mercapto and carboxylic acid. Suitable hydroxy protecting groups include trialkylsilyl or diarylalkylsilyl groups (e.g., t-butyldimethylsilyl, t-butyldiphenylsilyl or trimethylsilyl), tetrahydropyranyl, benzyl, allyl, and the like. Suitable protecting groups for amino, amidino, and guanidino include t-butoxycarbonyl, benzyloxycarbonyl, 9-fluorenylmethoxycarbonyl, benzyl, p-methoxybenzyl, allyl, allyloxycarbonyl, p-toluenesulfonyl, pivaloyl, trifluoroacetyl, and the like. Suitable mercapto-protecting groups include-C (O) -R "(wherein R" is alkyl, aryl or aralkyl), p-methoxybenzyl, trityl, and the like. Suitable carboxyl protecting groups include alkyl, aryl or aralkyl esters.

Protecting groups may be introduced and removed according to standard techniques known to those skilled in the art and as described herein. The use of protecting groups is described in detail in Greene, t.w. and p.g.m. wuts, protective Groups in Organi Synthesis, (1999), 4th Ed. The protecting group may also be a polymeric resin.

Typically, in the preparation scheme, each reaction is carried out in an inert solvent at room temperature to reflux temperature (e.g., 0 ℃ to 150 ℃, preferably 10 ℃ to 100 ℃). The reaction time is usually 0.1 hours to 60 hours, preferably 0.5 to 48 hours.

Preferably, the structure of the compound of the formula II is shown as a formula II-1, and the compound of the formula II can be prepared by the following method:

(1) Reacting the compound a with the compound b to generate a compound c;

(2) The compound c undergoes a reduction reaction in the presence of a reducing agent selected from the group consisting of: hydrogen, sodium borohydride, lithium borohydride, potassium borohydride, diisobutylaluminum hydride, lithium aluminum hydride, sodium dihydro-bis (2-methoxyethoxy) aluminate, or a combination thereof;

(3) Reacting compound d with compound d-2 in the presence of a base to form compound e, wherein the base is selected from the group consisting of: sodium hydride, potassium t-butoxide, sodium hydroxide, potassium hydroxide, n-butyllithium, lithium diisopropylamide, lithium hexamethyldisilazide, sodium hexamethyldisilazide, potassium carbonate, cesium carbonate, sodium carbonate, triethylamine, diisopropylethylamine, 1.8-diazabicyclo [5.4.0] undec-7-ene, or a combination thereof;

(4) Removing the protecting group from the compound e to generate a compound II-1;

wherein R is ⁵ 、R ⁶ 、R ⁹ 、R ¹⁰ Is defined as above.

Preferably, the structure of the compound of the formula I is shown as a formula I-1, and the compound can be prepared by the following method:

(1) In the presence of a condensing agent, performing condensation reaction on the compound II-1-1 and f1 to generate a compound g1;

(2) In the presence of a condensing agent, performing condensation reaction on the compound g1 and h1 to generate a compound i1;

(3) In the presence of a condensing agent, the compound i1 is subjected to self-condensation reaction to generate a compound j1;

(4) Compound j1 and corresponding contained R ¹ Alkylating or acylating the reagent to generate a compound I-1;

the condensing agent is selected from the following group: dicyclohexylcarbodiimide, diisopropylcarbodiimide, 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride, 1-hydroxy-7-azobenzotriazole, 1-hydroxybenzotriazole, N-hydroxysuccinimide, N '-tetramethyl-O- (7-azabenzotriazol-1-yl) hexafluorophosphate urea, benzotriazol-N, N' -tetramethylurea hexafluorophosphate, 1H-benzotriazol-1-yloxytris (dimethylamino) phosphonium hexafluorophosphate, propylphosphoric anhydride, 2-hydroxypyridine nitrogen nitride, 1H-benzotriazol-1-yloxytripyrrolidinyl hexafluorophosphate, or a combination thereof;

Wherein L is ³ Is absent or-CR ^a R ^b -；R ¹ 、R ² 、R ³ 、R ^a 、R ^b And n is as defined above.

Preferably, the structure of the compound of the formula I is shown as a formula I-2, and the compound can be prepared by the following method:

(1) In the presence of a condensing agent, performing condensation reaction on the compound II-1-1 and f2 to generate a compound g2;

(2) In the presence of a condensing agent, performing condensation reaction on the compound g2 and h1 to generate a compound i2;

(4) In the presence of a condensing agent, the compound i2 is subjected to self-condensation reaction to generate a compound j2;

(5) Compound j2 and corresponding contained R ¹ Alkylating or acylating the reagent to generate a compound I-2;

wherein L is ³ Is absent or-CR ^a R ^b -; b ring, R ¹ 、R ^a 、R ^b The definition of n and condensing agent is as above.

Preferably, the structure of the compound of the formula I is shown as a formula I-3, and the compound can be prepared by the following method:

(1) In the presence of a condensing agent, performing condensation reaction on the compound II-1-2 and f1 to generate a compound g3;

(2) In the presence of a condensing agent, performing condensation reaction on the compound g3 and h2 to generate a compound i3;

(3) In the presence of a condensing agent, the compound i3 is subjected to self-condensation reaction to generate a compound j3;

(4) Compound j3 and corresponding contained R ¹ Alkylating or acylating the reagent to generate a compound I-3;

wherein L is ¹ is-CR ^a R ^b -；R ¹ 、R ² 、R ³ 、R ^a 、R ^b And n is as defined above.

Preferably, the structure of the compound of the formula I is shown as a formula I-4, and the compound can be prepared by the following method:

(1) In the presence of a condensing agent, performing condensation reaction on the compound II-1-2 and f2 to generate a compound g4;

(2) In the presence of a condensing agent, performing condensation reaction on the compound g4 and h2 to generate a compound i4;

(4) In the presence of a condensing agent, the compound i4 is subjected to self-condensation reaction to generate a compound j4;

(5) Compound j4 and corresponding contained R ¹ The reagent is alkylated or acylatedForming a compound I-4;

wherein L is ¹ is-CR ^a R ^b -; b ring, R ¹ 、R ^a 、R ^b The definition of n and condensing agent is as above.

Compared with the prior art, the invention has the following beneficial effects:

(1) The invention provides a compound shown as a formula I or pharmaceutically acceptable salt thereof;

(2) The invention provides a compound shown as a formula I for preparing a pharmaceutical composition for preventing and/or treating diseases caused by coronavirus infection, including SARS-CoV-2, SARS-CoV-1, MERS-CoV, HCoV, TGEV, MHV, BCoV, IBV and the like.

The technical scheme of the invention is further described by the following specific embodiments. It will be apparent to those skilled in the art that the examples are merely to aid in understanding the invention and are not to be construed as a specific limitation thereof.

The experimental methods, in which specific conditions are not noted in the following examples, are generally conducted under conventional conditions or under conditions recommended by the manufacturer. Percentages and parts are weight percentages and parts unless otherwise indicated.

The experimental materials and reagents used in the following examples were obtained from commercial sources unless otherwise specified.

In the context of the various embodiments of the present invention, ¹ h NMR was recorded by BRUKER AVANCE NEO 400.400 MHz NMR and chemical shifts were expressed as δ (ppm); LC-MS (LCMS) was recorded by Shimadzu LC-20AD, SIL-20A, CTO-20AC, SPD-M20A, CBM-20A, LCMS-2020 type mass spectrometer; preparative HPLC separation was performed using a Gilson-281 model liquid chromatograph.

Examples

Preparation of intermediates

1. Preparation of intermediate A

The synthetic route for intermediate a is shown below:

(1) To an aqueous solution of sodium hydroxide (0.50 mol/L,59.3 mL) at 0℃were added the hydrochloride salt of Compound A-1 (5.00 g,29.6 mmol) and copper sulfate pentahydrate (3.70 g,14.8 mmol), and the reaction solution was stirred at 25℃for 0.5 hours. Potassium carbonate (4.10 g,29.6 mmol) and benzyl chloroformate (6.58 g,38.5mmol,5.48 mL) were added while ice-bath, and the reaction was stirred for an additional 0.5 hr at 25 ℃. The mixture was filtered, and the cake was washed with methanol (25.0 mL. Times.2), an aqueous solution of sodium hydroxide (0.25 mol/L,118 mL) and ethylenediamine tetraacetic acid (4.33 g,14.8 mmol), heated to 95℃and stirred for 2 hours. Filtration and washing of the filter cake with water (30.0 mL. Times.3) gave compound A-2.

MS-ESI[M+H] ⁺ Calculated 267, found 267.

(2) To a solution of Compound A-2 (6.50 g,24.4 mmol) in methanol (50.0 mL) was added an aqueous solution of sodium hydroxide (0.50 mol/L,53.7 mL) and di-tert-butyl carbonate (7.99 g,36.6 mmol), and the reaction was stirred at 25℃for 12 hours. The mixture was filtered, the filtrate was washed with ethyl acetate (30.0 mL. Times.2), pH was adjusted to 2 by adding aqueous hydrochloric acid (2.0 mol/L), extraction was performed with ethyl acetate (30.0 mL. Times.2), and the combined organic phases were washed with saturated brine (20.0 mL. Times.2), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give Compound A.

¹ H NMR(400MHz,DMSO-d6)δ12.37(br,1H),7.29-7.38(m,5H),7.22-7.25(m,1H),7.05(d,J＝8.0Hz,1H),5.01(s,2H),3.82-3.85(m,1H),2.98(dd,J＝6.0,12.0Hz,2H),1.64-1.66(m,1H),1.44-1.54(m,2H),1.38(s,9H)。

2. Preparation of intermediate B

The synthetic route for intermediate B is shown below:

(1) To a solution of compound B-1 (50.0 g,387 mmol) in acetone (500 mL) was added triethylamine (45.0 g,445 mmol) and benzyl bromide (69.5 g,406mmol,48.3 mL), and the reaction was stirred at 25℃for 6 hours. The reaction solution was concentrated under reduced pressure, ice water (300 mL) was added, extraction was performed with ethyl acetate (200 mL. Times.3), and the organic phase was combined, washed with water (300 mL. Times.1) and saturated brine (200 mL. Times.1), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give Compound B-2.

(2) To a solution of compound B-2 (68.0 g,310 mmol) in methylene chloride (800 mL) at 0deg.C was added 4-dimethylaminopyridine (7.58 g,62.0 mmol) and di-tert-butyl carbonate (74.4 g, 3411 mmol), and the reaction was stirred at 25deg.C for 16 hours. The reaction was quenched with ice water (500 mL), extracted with dichloromethane (200 mL. Times.1), and the combined organic phases were washed with water (200 mL. Times.1) and saturated brine (200 mL. Times.1), dried over anhydrous sodium sulfate, filtered, and the organic phase was concentrated under reduced pressure to give compound B-3.

(3) To a solution of compound B-3 (50.0 g,156 mmol) in ethylene glycol dimethyl ether (800 mL) was added tert-butoxybis (dimethylamino) methane (40.9 g,234 mmol), and the reaction was stirred under nitrogen at 85℃for 12 hours. The reaction solution was concentrated under reduced pressure, ethyl acetate (30.0 ml×1) was added, the organic phase was washed with water (30.0 ml×2) and saturated brine (20.0 ml×1), dried over anhydrous sodium sulfate, filtered, and the organic phase was concentrated under reduced pressure, and the crude product was separated by silica gel column chromatography (petroleum ether/ethyl acetate=1:1 to 1:4) to give compound B-4.

MS-ESI[M+H-Boc] ⁺ Calculated 275, measured 275.

¹ H NMR(400MHz,CDCl ₃ )δ7.31-7.36(m,5H),7.12(s,1H),5.13-5.23(m,2H),4.57(dd,J＝3.6,10.4H,1H),3.19-3.26(m,1H),2.99(s,6H),2.83-2.88(m,1H),1.42(s,9H)。

(4) To a solution of Compound B-4 (43.0 g,114 mmol) in tetrahydrofuran (4.0L) was added diisobutylaluminum hydride (1 mol/L,172 mL) at-65℃and the reaction was stirred under nitrogen for 1 hour at-65 ℃. The temperature was raised to 0℃and the reaction was continued for 1 hour. The reaction was quenched by the addition of water (6.88 mL), sodium hydroxide (15%, 6.88 mL), water (20.6 mL) and magnesium sulfate (40.0 g), stirring was continued for 30 minutes, the reaction solution was filtered, water (600 mL) was added, extracted with ethyl acetate (200 mL. Times.2), the organic phase was washed with saturated brine (200 mL. Times.1), dried over anhydrous sodium sulfate, filtered, the organic phase was concentrated under reduced pressure, and the crude product was isolated by silica gel column chromatography (petroleum ether/ethyl acetate=10:1) to give compound B-5.

¹ H NMR(400MHz,CDCl ₃ )δ7.32-7.37(m,5H),6.23(t,J＝2.4H,1H),5.50(t,J＝2.4H,1H),5.19(dd,J＝12.0,28.4H,2H),4.65(dd,J＝3.2,6.0H,1H),3.01-3.10(m,1H),2.66-2.72(m,1H),1.44(s,9H)。

(5) To a solution of compound B-5 (12.0 g,36.2 mmol) in tetrahydrofuran (120 mL) was added 1.8-diazabicyclo [5.4.0] undec-7-ene (553mg, 3.62 mmol) and compound B-6 (15.9 g,72.4 mmol), and the reaction was stirred at 25℃for 16 hours. The reaction mixture was added with water (200 mL), extracted with ethyl acetate (100 ml×2), the organic phase was washed with saturated brine (50.0 ml×1), dried over anhydrous sodium sulfate, filtered, and the organic phase was concentrated under reduced pressure, and the crude product was separated by silica gel column chromatography (petroleum ether/ethyl acetate=1:0 to 6:1) to give compound B-7.

MS-ESI[M+H] ⁺ Calculated 552, measured 552.

¹ H NMR(400MHz,CDCl ₃ )δ7.46-7.81(m,6H),7.29-7.38(m,7H),7.15-7.22(m,2H),5.14-5.24(m,2H),4.51-4.62(m,2H),2.78-3.05(m,1H),2.33-2.49(m,2H),2.08-2.14(m,2H),1.40-1.44(m,9H)。

(6) To a solution of Compound B-7 (12.9 g,23.3 mmol) in tetrahydrofuran (130 mL) was added aqueous hydrochloric acid (1 mol/L,25.7 mL), and the reaction was stirred at 25℃for 1 hour. The reaction mixture was washed with methyl t-butyl ether (40.0 mL. Times.3), the pH of the aqueous phase was adjusted to 10 with aqueous sodium hydroxide (1 mol/L), extracted with methylene chloride (30.0 mL. Times.3), the organic phase was washed with saturated brine (50.0 mL. Times.1), dried over anhydrous sodium sulfate, filtered, the organic phase was concentrated under reduced pressure, and the crude product was separated by silica gel column chromatography (methylene chloride/methanol=50:1) to give compound B.

¹ H NMR(400MHz,CDCl ₃ )δ7.33-7.36(m,5H),5.12-5.26(m,2H),4.45-4.64(m,1H),4.09-4.38(m,1H),2.91-2.97(m,1H),2.59-2.66(m,1H),2.24-2.51(m,3H),1.39-1.44(m,9H)。

Example 1 preparation of Compound 1

The synthetic route for compound 1 is shown below:

(1) To a solution of compound B (3.82 g,14.9 mmol) in dichloromethane (40.0 mL) was added 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (3.62 g,18.8 mmol), diisopropylethylamine (4.07 g,31.4 mmol), 6-chloro-1-hydroxybenzotriazole (2.55 g,18.8 mmol) and compound 1-1 (6.10 g,15.7 mmol), and the reaction was stirred at 25℃for 12 hours. The reaction mixture was added with water (50.0 mL), extracted with dichloromethane (30.0 ml×2), the combined organic phases were washed with dilute hydrochloric acid (1 mol/L,20.0ml×1), saturated aqueous sodium carbonate (20.0 ml×1) and saturated brine (20.0 ml×1), dried over anhydrous sodium sulfate, filtered, and the organic phase was concentrated under reduced pressure, and the crude product was separated by silica gel column chromatography (dichloromethane/methanol=50:1) to give compound 1-2.

MS-ESI[M-H] ^- Calculated value 623, measured value 623.

¹ H NMR(400MHz,CDCl ₃ )δ7.37(m,5H),5.14-5.23(m,2H),4.92-5.09(m,1H),4.50-4.73(m,1H),4.02-4.12(m,1H),3.39-3.78(m,2H),2.60-2.71(m,1H),2.30-2.46(m,1H),1.90-2.10(m,2H),1.66-1.69(m,1H),1.33-1.43(m,19H),1.25-1.31(m,1H),0.87-1.05(m,6H)。

(2) To a solution of compound 1-2 (2.00 g,3.20 mmol) in dichloromethane (30.0 mL) was added trifluoroacetic acid (5.0 mL), and the reaction was stirred at 25℃for 2 hours. The reaction solution was concentrated under reduced pressure, the pH was adjusted to 9 with a saturated sodium carbonate solution, extracted with methylene chloride (20.0 mL. Times.1), the organic phase was washed with saturated brine (20.0 mL. Times.1), dried over anhydrous sodium sulfate, filtered, and the organic phase was concentrated under reduced pressure to give Compound 1-3.

MS-ESI[M+H] ⁺ Calculated 425, measured 425.

(3) To a solution of compound 1-3 (750 mg,2.05 mmol) in dichloromethane (15.0 mL) was added 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (560 mg,2.93 mmol), diisopropylethylamine (758 mg,5.87 mmol), 6-chloro-1-hydroxybenzotriazole (390 mg,2.93 mmol) and compound A (830 mg,1.96 mmol), and the reaction was stirred at 25℃for 12 hours. The reaction solution was added with water (30.0 mL), extracted with methylene chloride (15.0 ml×2), the combined organic phases were washed with dilute hydrochloric acid (1 mol/L,10.0ml×1), saturated aqueous sodium carbonate solution (10.0 ml×1) and saturated brine (10.0 ml×1), dried over anhydrous sodium sulfate, filtered, and the organic phase was concentrated under reduced pressure, and the crude product was separated by silica gel column chromatography (methylene chloride/methanol=50:1 to 30:1) to give compounds 1-4.

MS-ESI[M-H] ^- Calculated 771, measured 771.

(4) To a solution of compounds 1 to 4 (260 mg, 336. Mu. Mol) in methanol (5.0 mL) were added tetrahydrofuran (1.5 mL) and palladium on carbon (0.1 g, 10%), and the reaction solution was stirred at 25℃for 12 hours under a hydrogen atmosphere. The reaction solution was filtered, and the filtrate was concentrated under reduced pressure to obtain compounds 1-5.

¹ H NMR(400MHz,CDCl ₃ )δ4.12-4.35(m,2H),3.82-4.03(m,3H),2.87-3.01(m,2H),2.65-2.76(m,1H),2.36-2.49(m,1H),1.91-2.17(m,2H),1.50-1.79(m,6H),1.41(s,9H),1.30-1.37(m,2H),0.88-1.17(m,6H)。

(5) To a solution of compound 1-5 (180 mg, 328. Mu. Mol) in N, N-dimethylformamide (36.0 mL) was added 2- (7-azobenzotriazole) -N, N, N, N-tetramethylurea hexafluorophosphate (149 mg, 393. Mu. Mol), diisopropylethylamine (55.1 mg, 426. Mu. Mol) and 3H- [1,2,3] triazolo [4,5-b ] pyridin-3-ol (53.5 mg, 393. Mu. Mol), and the reaction was stirred at 25℃for 16 hours. The reaction solution was concentrated under reduced pressure, aqueous citric acid (6.0 mL, 4%) was added, extracted with ethyl acetate (15.0 ml×3), and the combined organic phases were washed with saturated aqueous sodium bicarbonate (10.0 ml×1) and saturated brine (10.0 ml×1), dried over anhydrous sodium sulfate, filtered, the organic phase was concentrated under reduced pressure, and the crude product was separated by reverse phase column chromatography (acetonitrile/water=35%) to give compounds 1 to 6.

MS-ESI[M-H] ^- Calculated 529, measured 529.

(6) To a solution of compounds 1-6 (9.00 mg, 16.9. Mu. Mol) in methylene chloride (2.0 mL) was added trifluoroacetic acid (0.5 mL), and the reaction was stirred at 25℃for 2 hours. The reaction solution was concentrated under reduced pressure to obtain the trifluoroacetate salt of Compound 1-7.

(7) Triethylamine (6.69 mg, 66.1. Mu. Mol) and trifluoroacetic anhydride (5.21 mg, 24.7. Mu. Mol) were added to a solution of the trifluoroacetate salt of compounds 1-7 (9.0 mg, 16.5. Mu. Mol) in methylene chloride (5.0 mL) at 0℃and the reaction mixture was stirred at 25℃for 2 hours. The reaction was quenched by the addition of water (10.0 mL), extracted with dichloromethane (10.0 mL. Times.2), the organic phase was washed with saturated brine (10.0 mL. Times.1), dried over anhydrous sodium sulfate, filtered, and the organic phase was concentrated under reduced pressure, and the crude product was isolated by preparative high performance liquid chromatography (YMC Triart,150 mm. Times.30 mm. 7. Mu.m, A: water (10 mmol/L ammonium bicarbonate); B: acetonitrile, 0% -60%:40 min) to give Compound 1.

MS-ESI[M-H] ^- Calculated 525, measured 525.

¹ H NMR(400MHz,MeOD)δ5.01(dd,J＝2.4,12.8Hz,1H),4.49(dd,J＝3.2,10.4Hz,1H),4.13(s,1H),4.01(d,J＝9.2Hz,1H),3.86-3.94(m,2H),3.78-3.81(m,1H),3.47-3.52(m,1H),2.91-2.97(m,1H),2.57-2.63(m,2H),2.36-2.43(m,1H),2.15-2.18(m,1H),1.99-2.07(m,1H),1.71-1.83(m,2H),1.58-1.65(m,2H),1.38-1.39(m,1H),1.07(s,3H),0.95-0.96(m,3H)。

Test example Compound pair SARS-COV-2-3 CL ^pro Inhibition of target

The purpose of the experiment is as follows: detection of SARS-COV-2-3 CL by test Compound ^pro Inhibition effect of target spot.

Experimental materials: the test medicine is prepared by the compound of the embodiment of the invention; SARS-COV-2 3CL ^pro FRET polypeptides are available from GL-China (cat. No. 787985) from Shanghai Runno Biotech Co., ltd; dimethyl sulfoxide was purchased from Sigma (cat No. D4540); optiPlate-384 was purchased from Perkinelmer (cat. No. 6007270); centrifuges were purchased from Eppendorf (model 5430); the microplate reader was purchased from Biotek (model number Synergy 2); echo 550 was purchased from Labcyte (model Echo 550).

The experimental method comprises the following steps: the test compound was dissolved in dimethylsulfoxide to prepare a stock solution at a concentration of 10mM, starting at a final concentration of 10. Mu.M, diluted 3 times, and 10 concentrations. Transfer 20nL 1000-fold final concentration of test compound solution to 384 wells using Echo 550In the reaction plate. 20nL of dimethyl sulfoxide was added to the blank wells and the negative control wells. mu.L of a 1-fold final concentration of the reaction solution (50 mM Tris-HCI) pH 7.5,1M sodium sulfate, 1mM EDTA, 0.01% fetal bovine serum) was added to the blank wells, and 10. Mu.L of 10nM SARS-COV-2 3CL was added to the remaining wells ^pro A solution. Centrifuge at 1000rpm for 1 min and incubate at room temperature for 15 min. 10. Mu.L of 30. Mu.M FRET polypeptide was added to each well of the reaction plate, and the reaction was started. The reaction plate was centrifuged at 1000rpm for 1 minute and the fluorescent signal was continuously read using Synergy 2. Log (inhibitor) vs. response-Variable slope fit using analytical software GraphPad Prism 5 to derive IC for inhibition of enzyme activity for each compound ₅₀ Values. The test results are shown in Table 1.

TABLE 1

Examples	IC 50 (nM)
		1	30

As can be seen from the test data in Table 1, the cyclopeptide compound represented by formula I of the present invention is effective against SARS-COV-2 3CL ^pro Has remarkable inhibiting effect and has the potential of being used for preparing medicines for treating and preventing diseases caused by coronavirus infection such as SARS-CoV-2, SARS-CoV-1, MERS-CoV, HCoV, TGEV, MHV, BCoV, IBV and the like.

The applicant states that the cyclic peptide compounds, pharmaceutical compositions comprising them and their use are illustrated by the above examples, but the invention is not limited to, i.e. it is not meant that the invention must be practiced in dependence upon the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.

Claims (12)

1. A cyclic peptide compound of formula I, or a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuterate or combination thereof,

wherein,,

a is selected from the sequences:

wherein the N atom in the sequence is connected with a carbonyl group, and the carbonyl group in the sequence is connected with the N atom;

R ² selected from: H. C1-C6 alkyl, C3-C8 cycloalkyl, C2-C6 alkenyl, C4-C6 cycloalkenyl, C3-C8 cycloalkyl-CH ₂ -and C4-C6 cycloalkenyl-CH ₂ -said alkyl, alkenyl, cycloalkyl, cycloalkenyl optionally substituted by halogen, C1-C3 alkyl, C1-C3 haloalkyl or C2-C4 alkenyl;

R ³ Selected from: H. C1-C4 alkyl and C1-C4 haloalkyl;

ring B is an optionally substituted 3-12 membered heterocycle; wherein the substituents are selected from: C1-C3 alkyl, C1-C3 haloalkyl or halogen;

R ¹ selected from: C1-C6 alkyl, C3-C8 cycloalkyl, C6-C10 aryl, 5-to 10-membered heteroaryl, C1-C6 alkyl-CO-, C3-C8 cycloalkyl-CO-, 3-to 12-membered heterocyclyl-CO-, C6-C10 aryl-CO-, 5-to 10-membered heteroaryl-CO-, C1-C4 alkyloxy-CO-, C1-C4 alkylamino-CO-, C1-C6 alkyl-SO ₂ -, a part of C3-C8 cycloalkyl-SO ₂ -and 3-12 membered heterocyclyl-SO ₂ -; wherein the alkyl, cycloalkyl, heterocyclyl, aryl and heteroaryl groups may optionally beSubstituted with halogen, C1-C3 alkyl, C1-C3 alkoxy, formylamino, C1-C3 alkylamino, C3-C8 cycloalkyl, 3-C12 membered heterocyclyl, C6-C10 aryl, or 5-10 membered heteroaryl; wherein said alkyl, formylamino, alkylamino, cycloalkyl, heterocyclyl, aryl and heteroaryl groups are optionally substituted with halogen, C1-C3 alkyl, C1-C3 alkoxy, hydroxy, amino, (C1-C3 alkyl) ₂ Amino, C1-C3 haloalkoxy, C3-C8 cycloalkyl, C3-C8 halocycloalkyl, 3-12 membered heterocyclyl, 3-12 membered haloheterocyclyl, C6-C10 aryl, or 5-10 membered heteroaryl substitution;

L ¹ Selected from: -CO-and-CR ^a R ^b -; and when L ¹ In the case of-CO-, L ² is-NR ^c -, or-O-, L ³ is-CR ^a R ^b -; when L ¹ is-CR ^a R ^b -when, L ² is-NR ^c -, -O-or-CR ^a R ^b -，L ³ Is absent, -CO-or-CR ^a R ^b -；

Each R is ^a Independently selected from: absence, H, halogen, -OH, oxo, mercapto, cyano, -NH ₂ Optionally substituted C1-C6 alkyl, optionally substituted C1-C6 alkoxy, optionally substituted 3-8 membered cycloalkyl or optionally substituted 4-8 membered heterocyclyl, or R ^a And R is R ^b Is linked to C to form an optionally substituted 3-8 membered carbocyclic ring or 4-8 membered heterocyclic ring; wherein the heterocycle comprises 1-3 heteroatoms selected from N, O, S, P; wherein the substituted substituent is R;

each R is ^b Independently selected from: absence, H, halogen, -OH, oxo, mercapto, cyano, -NH ₂ Optionally substituted C1-C6 alkyl, optionally substituted C1-C6 alkoxy, optionally substituted 3-8 membered cycloalkyl or optionally substituted 4-8 membered heterocyclyl, or R ^a And R is R ^b Is linked to C to form an optionally substituted 3-8 membered carbocyclic ring or 4-8 membered heterocyclic ring; wherein the heterocycle comprises 1-3 heteroatoms selected from N, O, S, P; wherein the substituted substituent is R;

each R is independently selected from: halogen, -OH, oxo (= O), -NH ₂ C3-C8 cycloalkyl, 3-8 membered heterocyclyl, C1-C4 alkyl or C1-C4 haloalkyl-;

R ^c selected from: h or C1-C3 alkyl;

n is 2, 3 or 4.

2. The compound of claim 1, or a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuterate, or combination thereof,

a is selected from the sequences:

the N atoms in the sequence are connected with carbonyl groups, and the carbonyl groups in the sequence are connected with the N atoms;

R ³ selected from: H. C1-C4 alkyl or C1-C4 haloalkyl.

3. The compound of claim 1 or 2, or a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuterate or combination thereof,

R ¹ the ring is selected from any one of the following groups:

4. the compound of any one of claim 1 to 3, or a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuterate or combination thereof,

each R is ^a Selected from: H. halogen or methyl.

5. The compound of any one of claim 1 to 4, or a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuterate or combination thereof,

Each R is ^b Selected from: H. halogen or methyl.

6. The compound of any one of claims 1-5, or a pharmaceutically acceptable salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph, deuterate, or combination thereof, wherein the compound is selected from the group consisting of:

7. a compound of formula II, or a salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph or deuterate thereof,

wherein,,

R ⁴ is that

R ⁷ And R is ⁸ Each independently selected from: H. C1-C6 alkyl, C2-C4 alkenyl, C1-C6 alkyl-CO-, C3-C8 cycloalkyl-CO-, C1-C6 alkyl-SO ₂ -, C1-C6 alkyl-SO-; 5-10 membered aryl-CO-, 5-10 membered aryl-SO ₂ -, 5-12 membered heteroaryl-CO-, 5-12 membered heteroaryl-SO ₂ -, C1-C6 alkyl-O-CO-; 5-10 membered aryl-CH ₂ -O-CO-or 5-12 membered heteroaryl-CH ₂ -O-CO-, said alkyl, alkenyl, cycloalkyl, aryl and heteroaryl groups optionally being substituted with one or more substituents selected from the group consisting of: halogen, C1-C3 alkyl, C3-C8 cycloalkyl, C1-C3 alkoxy or phenyl, wherein said phenyl is optionally substituted with one or more substituents selected from the group consisting of: halogen, methyl or methoxy; or R is ⁷ And R is R ⁸ And together with the N atoms to which they are attached form a 4-8 membered heterocyclic ring optionally substituted with a substituent of group X; wherein the heterocyclic ring comprises 0-2 heteroatoms selected from N, O, S or P in addition to the attached N atom;

R ⁹ and R is ¹⁰ Each independently selected from: a C1-C6 alkyl, 5-10 membered aryl or 5-12 membered heteroaryl, said alkyl, aryl, heteroaryl optionally substituted with a substituent of group X;

R ⁵ is that

R ¹¹ Selected from: H. C1-C6 alkyl, C2-C4 alkenyl, C3-C6 cycloalkyl, benzyl orThe alkyl, alkenyl, cycloalkyl and benzyl groups may be optionally substituted with one or more substituents selected from the group consisting of: halogen, methyl or methoxy;

R ^11a 、R ^11b and R is ^11c Each independently selected from: C1-C4 alkyl or phenyl;

R ¹² and R is ¹³ Each independently selected from: H. C1-C6 alkyl, C2-C4 alkenyl, C1-C6 alkyl-CO-, C3-C8 cycloalkyl-CO-, C1-C6 alkyl-SO ₂ -, C1-C6 alkyl-SO-; 5-10 membered aryl-CO-, 5-10 membered aryl-SO ₂ -, 5-12 membered heteroaryl-CO-, 5-12 membered heteroaryl-SO ₂ -, C1-C6 alkyl-O-CO-; 5-10 membered aryl-CH ₂ -O-CO-, 5-12 membered heteroaryl-CH ₂ -O-CO-or C2-C4 alkenyl-O-CO-, said alkyl, alkenyl, cycloalkyl, aryl and heteroaryl groups optionally being substituted with one or more substituents selected from the group consisting of: halogen, C1-C3 alkyl, C3-C8 cycloalkyl, C1-C3 alkoxy or phenyl, said phenyl optionally being substituted with one or more substituents selected from the group consisting of: halogen, methyl and methoxy; or R is ¹² And R is R ¹³ And together with the N atoms to which they are attached form a 4-8 membered heterocyclic ring optionally substituted with a substituent of group X; wherein the heterocyclic ring comprises 0-2 heteroatoms selected from N, O, S or P in addition to the attached N atom;

the substituents of group X are selected from: oxo, halogen, C1-C6 alkyl or C1-C6 alkoxy;

R ⁶ selected from: H. C1-C6 alkyl, C2-C4 alkenyl, C1-C6 alkyl-SO ₂ -, 5-to 10-membered aryl-SO ₂ -, C1-C6 alkyl-O-CO-; C2-C4 alkenyl-O-CO-, 5-10 membered aryl-CH ₂ -O-CO-or 5-12 membered heteroaryl-CH ₂ -O-CO-, said alkyl, alkenyl, cycloalkyl, aryl and heteroaryl groups optionally being substituted with one or more substituents selected from the group consisting of: halogen, C1-C3 alkyl, C3-C8 cycloalkyl, C1-C3 alkoxy or phenyl, said alkoxy and phenyl optionally being substituted with one or more substituents selected from the group consisting of: halogen, methyl, methoxy, nitro or trimethylsilyl.

8. The compound, or a salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph or deuterate thereof according to claim 7 wherein R ⁴ Selected from: amino or amino groups

9. The compound according to any one of claims 7 to 8, or a salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph or deuterate thereof, wherein R ⁵ Selected from:

10. the compound according to any one of claims 7 to 9, or a salt, enantiomer, diastereomer, tautomer, cis-trans isomer, solvate, polymorph or deuterate thereof, wherein R ⁶ Selected from: boc, allyl, benzyl or p-methoxybenzyl.

11. A pharmaceutical composition comprising a compound according to any one of claims 1-6 and a pharmaceutically acceptable carrier.

12. Use of a compound according to any one of claims 1 to 6 or a pharmaceutical composition according to claim 11, wherein the use is selected from any one of the following (a) - (c):

(a) Preparation of a protease (M) useful for prophylaxis and/or treatment and a primary protease (M) ^pro ) Or 3C-like protease (3 CL) ^pro ) Medicaments for related diseases associated with viral infections;

(b) Preparation of the protease (M) for in vitro non-therapeutic and Main protease(s) ^pro ) Or 3C-like protease (3 CL) ^pro ) An inhibitor related to the activity of (a); or (b)

(c) Preparation of proliferation inhibitors for in vitro non-therapeutic coronaviruses.