CN116850128B

CN116850128B - A microecological regulation composition, preparation method and application

Info

Publication number: CN116850128B
Application number: CN202311058954.3A
Authority: CN
Inventors: 顾镕; 潘家辉; 赵金; 刘新琦; 吕晨媛
Original assignee: Shanghai Zunya Industrial Co ltd
Current assignee: Shanghai Zunya Industrial Co ltd
Priority date: 2023-08-22
Filing date: 2023-08-22
Publication date: 2025-02-07
Anticipated expiration: 2043-08-22
Also published as: CN116850128A

Abstract

本申请涉及化妆品技术领域，具体公开了一种微生态调节组合物、制备方法及应用。一种微生态调节组合物，包含以下重百分比的组分：乳酸杆菌发酵溶胞产物0.1‑5%；乳酸杆菌/豆浆发酵产物滤液0.05‑2%；α‑葡聚糖寡糖0.1‑10%；复配植物提取物0.2‑20%；保湿剂1‑35%；溶剂0.1‑10%；余量为去离子水；其制备方法为：将乳酸杆菌发酵溶胞产物、乳酸杆菌/豆浆发酵产物滤液、α‑葡聚糖寡糖、复配植物提取物、保湿剂、溶剂和水搅拌混合均匀即可得到。本申请的微生态调节组合物，能够有效维持皮肤微生态平衡，对痘痘有突出的改善作用。The present application relates to the field of cosmetic technology, and specifically discloses a microecological regulation composition, a preparation method and an application. A microecological regulation composition, comprising the following weight percentage components: lactobacillus fermentation lysate 0.1-5%; lactobacillus/soy milk fermentation product filtrate 0.05-2%; α-glucan oligosaccharide 0.1-10%; compound plant extract 0.2-20%; moisturizer 1-35%; solvent 0.1-10%; the remainder is deionized water; its preparation method is: lactobacillus fermentation lysate, lactobacillus/soy milk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water are stirred and mixed to obtain. The microecological regulation composition of the present application can effectively maintain the balance of skin microecology and has a prominent improvement effect on acne.

Description

Microecological regulation composition, preparation method and application

Technical Field

The application relates to the technical field of cosmetics, in particular to a microecological regulating composition, a preparation method and application.

Background

In recent years, skin problems such as acne are frequently caused by multiple factors such as environmental and life pressure. Acne is commonly known as acne, and is called "whelk", "pimple" or "comedo", and is a skin disease caused by obstruction of hair follicles and sebaceous glands and inflammation. Generally, the face of a patient with acne is damaged by various skin lesions, and some may be more damaged, while others may be less damaged. Because cell turnover slows down with age, adult skin may take longer to heal than juvenile skin-meaning that signs and redness after acne may last longer, increasing the appearance of premature skin aging.

Microorganisms can be classified into resident flora, transient flora, and fungi, viruses, and mites according to the length of time they are colonized on the skin surface. Sebaceous glands and hair follicles are the main habitat of resident flora, form the first biological barrier of skin, and have the effects of maintaining balance and self-cleaning in the skin microecological environment. Propionibacterium acnes are resident bacteria colonized sebaceous glands, and understanding of the role of propionibacterium acnes in vaccinia pathophysiology has been shifted for nearly two years, not by hyperproliferation of propionibacterium acnes, but by imbalance between different propionibacterium acnes systems, and by deregulation of the skin microbiota, leading to the development of vaccinia. In fact, the skin microbial flora or the skin ecological flora is a major factor in skin immune protection. Any imbalance causes functional immunodeficiency, which often results in an area of skin being occupied by dormant pathogenic flora.

For the treatment of acnes, particularly moderate and severe acnes, the medical recommendation of the external product mainly comprises antibiotics, tretinoin, benzoyl peroxide, acids and traditional Chinese medicines, the conventional antibiotics are most frequently used for treating acnes, but the disadvantage of using antibiotics is obvious, allergic reaction can be possibly caused, meanwhile, bacterial flora generates drug resistance to medicines, for example, the pathogenicity of the acnes can be improved after the bacterial flora is resistant to the medicines, and the balance of normal bacterial flora systems in the body can be broken again after a large amount of use for a long time, so that multiple infections are caused.

Therefore, there is a need to propose a solution to the above technical problems.

Disclosure of Invention

In order to regulate the microecological balance of skin and effectively improve acnes, the application provides a microecological regulating composition, a preparation method and application.

In a first aspect, the present application provides a microecological conditioning composition, which adopts the following technical scheme:

A microecological conditioning composition comprising the following components in weight percent:

0.1-5% of lactobacillus fermentation lysate;

0.05-2% of lactobacillus/soybean milk fermentation product filtrate;

0.1-10% of alpha-glucan oligosaccharides;

0.2-20% of compound plant extract;

1-35% of humectant;

0.1-10% of solvent;

the balance of deionized water.

By adopting the technical scheme, the lactobacillus fermentation lysate can inhibit the growth and propagation of candida albicans and propionibacterium acnes, can increase the relative abundance of beneficial bacteria such as lactobacillus, bifidobacterium, staphylococcus and the like, can reduce the relative abundance of harmful bacteria such as rocston bacteria, pseudomonas and the like, further effectively regulate the surface bacterial flora of the skin, inhibit the growth of pathogenic bacteria of the skin, maintain the micro-ecological balance of the skin and prevent invasion of exogenous bacteria, can improve the diversity of beneficial bacteria on the surface of the skin, maintain the weak acidity of skin health, strengthen the skin microbial barrier and chemical barrier, can selectively and competitively inhibit the proliferation of the harmful bacteria through organisms, regulate the diversity of the skin microbial flora, improve the relative abundance, balance the surface microbial flora of the skin, activate the expression of beta-defensin, inhibit the growth of pathogenic bacteria, thereby strengthening the skin microbial barrier, enable the skin to present a healthy state, and the application can improve the interaction of lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan, can play roles in improving the relative balance of the skin and the anti-acne, and can play roles of enhancing the interaction of the anti-acne, thereby improving the interaction of the skin and the anti-acne, thereby improving the relative activity, and the interaction of the skin and the anti-acne.

Preferably, the compound plant extract consists of a flaxseed extract and a Boerdoside tree leaf extract, and the weight ratio of the flaxseed extract to the Boerdoside tree leaf extract is (1.3-1.9): 1.

By adopting the technical scheme, the flaxseed extract has a relieving effect on inflammatory erythema caused by propionibacterium acnes, regulates the natural microbial community and the ecological system of the skin to normalize, promotes the synthesis of antimicrobial peptide AMPs, optimizes the physiological functions and microbial and chemical barrier functions of the skin, and better resists external invasion, the Boedodes leaf extract can specifically activate the expression of the natural antimicrobial peptide HBD-3 of the skin, inhibit the hyper-proliferation of propionibacterium acnes and staphylococcus aureus, inhibit the expression of inflammatory factors IL-8 and MIP 3-alpha, and relieve the acne red swelling, and the flaxseed extract and the Boedodes leaf extract are combined into a compound plant extract according to a specific weight range ratio, so that on one hand, the micro-ecological balance of the skin can be regulated, the acne can be effectively improved, on the other hand, the complex of lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan oligosaccharide can be well supported, and excellent skin repairing effects can be mutually exerted.

Preferably, the weight ratio of the flaxseed extract to the Boerdosia leaf extract is 1.5:1.

By adopting the technical scheme, the flaxseed extract and the Boerdosia leaf extract with the weight ratio have excellent mutual cooperation effect when in use, and can form a stable and excellent micro-ecological regulation system with lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan oligosaccharides, thereby effectively improving acnes.

Preferably, the humectant is one or a combination of more of butanediol, glycerol, propylene glycol, salicylic acid and hyaluronic acid;

the solvent is one or a combination of more of 1, 2-pentanediol, polyethylene glycol, dipropylene glycol, propanol ether and dipropyl alcohol oxide.

By adopting the technical scheme, the humectant and the solvent of the category have good dispersibility and suspension property, and can be stably combined with other component raw materials to form an excellent mixed system, so that the microecological regulating composition with excellent quality can fully play a role.

Preferably, the microecological regulating composition is prepared from 2-5% by weight of auxiliary agent including silica, nano graphene and beeswax, wherein the weight ratio of the silica to the nano graphene to the beeswax is (0.2-0.7): (0.1-0.3): 1.

By adopting the technical scheme, the silica has excellent protective effect on skin, is mainly reflected in physical protection, can effectively isolate sensitized substances, has obvious antibacterial effect on harmful bacteria, has good effect of preventing and improving vaccinia, has a certain anti-inflammatory effect on bee wax and good product stability, and forms auxiliary agents according to a specific proportion, and can combine lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan oligosaccharides on the silica and the nano graphene by utilizing the micro-conduction effect of the cooperation of the silica and the nano graphene and further can be quickly and deeply conducted to damaged parts of the skin, so that obvious synergistic effect is achieved on micro-ecological regulation of the skin, and the effect of the micro-ecological regulation composition in application is greatly improved.

Preferably, the weight ratio of the silica, the nano graphene and the beeswax is 0.4:0.2:1.

By adopting the technical scheme, the silica, the nano graphene and the beeswax with the weight ratio have relatively stable and excellent matching effect when in use, and can form a relatively uniform matching system with lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan oligosaccharide, thereby obtaining the microecological regulating composition with relatively excellent overall quality.

In a second aspect, the application provides a preparation method of a microecological regulation composition, which adopts the following technical scheme that the preparation method of the microecological regulation composition comprises the following steps:

(1) Preparing raw materials comprising lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate, alpha-glucan oligosaccharides, compound plant extracts, humectant, solvent and water according to a proportion;

(2) Uniformly stirring and mixing the lactobacillus fermentation lysate, the lactobacillus/soybean milk fermentation product filtrate, the alpha-glucan oligosaccharide, the compound plant extract, the humectant, the solvent and the water in the step (1), wherein the stirring temperature is 25-35 ℃, the stirring time is 5-20min, and the stirring speed is 300-500r/min, so as to obtain the microecological regulating composition.

By adopting the technical scheme, the preparation method is simple to operate, is convenient for large-scale production, and is beneficial to forming a stable microecological regulation system before the composition is applied to a product by mixing the raw materials, so that a relatively excellent action effect can be generated.

In a third aspect, the application provides application of a microecological conditioning composition, which adopts the following technical scheme that the application of the microecological conditioning composition comprises a skin care product obtained by applying the microecological conditioning composition.

By adopting the technical scheme, before the external coating product for treating the acnes, which is not mature in the traditional medicine, is used for microbiome modification substitution therapy, the ecological regulation composition disclosed by the application is applied to skin care products, can be used as a supplementary means for treating the acnes in the medical field, and fills up the gap in the field of skin care products.

Preferably, the skin care product comprises the following components in percentage by weight:

poloxamer 407 5-30%;

1-5% of 1, 3-butanediol;

0.5-10% of glycerol;

panthenol 0.1-1%;

0.2-1% of beta-glucan;

1-10% of nicotinamide;

0.2-3% of polyacrylate crosslinked polymer;

0.01-0.5% of sodium hydroxide;

0.1-10% of microecological regulating composition;

0.5 to 0.8 percent of p-hydroxyacetophenone;

0.5-0.8% of 1, 2-hexanediol;

the balance of deionized water.

By adopting the technical scheme, the microecological regulating composition is applied to the skin care product system, can be stably combined with other component raw materials to form a uniform mixed system, is mild in skin performance, and can regulate and balance skin microorganism barriers, inhibit pathogenic bacteria in skin microecology and effectively improve acnes when being applied.

In a fourth aspect, the application provides a preparation method of a skin care product, which adopts the following technical scheme:

A preparation method of a skin care product comprises the following steps:

(1) Preparing raw materials of A phase, B phase, C phase and D phase according to a proportion, wherein the A phase comprises poloxamer 407, 1, 3-butanediol and glycerin, the B phase comprises deionized water, panthenol, beta-glucan, nicotinamide and polyacrylate crosslinked polymer, the C phase comprises sodium hydroxide and a microecological regulating composition, and the D phase comprises p-hydroxyacetophenone and 1, 2-hexanediol:

(2) Mixing the phase A raw materials in the step (1), heating to 75-80 ℃ and stirring uniformly, mixing the phase B raw materials, heating to 75-80 ℃ and stirring uniformly, adding the phase B into the phase A, and stirring uniformly;

(3) And (3) cooling the mixture of the phase A and the phase B in the step (2) to 35-45 ℃, adding the raw materials of the phase C and the phase D, and uniformly stirring and mixing to obtain the skin care product. .

By adopting the technical scheme, the preparation process of the skin care product is simple, the skin care product is suitable for industrial production, and meanwhile, the raw materials of each component in the operation are added in a grading manner, so that the quality control is convenient in the process, and the skin care product with excellent and stable quality is further obtained.

In summary, the application has the following beneficial effects:

1. The application adopts the combination of lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan oligosaccharides, can inhibit the hyperproliferation of propionibacterium acnes, participate in the information communication and skin response reaction between skin and microorganisms, improve the diversity of skin flora and the relative abundance of beneficial bacteria, promote the production of antibacterial peptide, further strengthen skin microbial barrier, be favorable for maintaining skin microecological balance and have outstanding improving effect on vaccinia;

2. The application preferably adopts a compound plant extract composed of flaxseed extract and Boerdosia leaf extract according to a specific weight range ratio, on one hand, the microbial ecological balance of skin can be regulated, and vaccinia can be effectively improved, on the other hand, the compound of lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan oligosaccharides can be well supported, and excellent skin repairing effect can be mutually exerted;

3. The auxiliary agent composed of silica, nano graphene and beeswax according to a specific proportion is added, so that lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan oligosaccharide can be quickly and deeply conducted to the damaged part of skin, obvious synergistic effect is achieved on skin micro-ecological regulation, and the effect of the micro-ecological regulation composition in application is greatly improved.

Detailed Description

The present application will be described in further detail with reference to examples.

The raw materials used in the preparation examples and examples of the present application are all commercially available except for the specific descriptions:

The flaxseed extract is purchased from a product supplier, ashland, trade name LIPIGENINE;

Product suppliers of Boerdosia leaf extract are BASF, trade name A00067 Betapur;

Product suppliers of polyacrylate crosslinked polymers, sibirk, trade name SEPIMAZEN;

The particle size of the nano graphene is 300nm;

Commercial suppliers of beeswax CRODA, trade name SUPER REFINED BEESWAX-PA- (JP).

Examples

Example 1

The microecological regulating composition is prepared by the following raw materials and corresponding weights as shown in table 1:

Note that in the above steps, the stirring temperature is preferably 30 ℃, the stirring time is preferably 15min, the stirring speed is preferably 300r/min, the compound plant extract consists of a linseed extract and a boldo leaf extract according to the weight ratio of 1.5:1, the humectant is butanediol, and the solvent is 1, 2-pentanediol.

Examples 2 to 3

A microecological conditioning composition was different from example 1 in that the raw materials of the respective components and their respective weights are shown in Table 1.

TABLE 1 raw materials for each component and weight percentage thereof in examples 1-3

Example 4

A microecological regulating composition is different from the embodiment 1 in that the compound plant extract consists of flaxseed extract and Boerdosia leaf extract according to the weight ratio of 1.3:1.

Example 5

A microecological regulating composition is different from the embodiment 1 in that the compound plant extract consists of flaxseed extract and Boerdosia leaf extract according to the weight ratio of 1.9:1.

Example 6

A microecological regulating composition is different from the embodiment 1 in that the compound plant extract consists of flaxseed extract and Boerdosia leaf extract according to the weight ratio of 1.6:1.

Example 7

A microecological conditioning composition differs from example 1 in that the equivalent mass of the flaxseed extract is replaced by deionized water.

Example 8

A microecological conditioning composition differs from example 1 in that the equivalent mass of the Boldo leaf extraction is replaced by deionized water.

Example 9

A microecological regulating composition is different from the embodiment 1 in that the compound plant extract consists of flaxseed extract and Boerdosia leaf extract according to the weight ratio of 1.2:1.

Example 10

A microecological regulating composition is different from example 1 in that the compound plant extract consists of flaxseed extract and Boerdosia leaf extract in a weight ratio of 2.0:1.

Example 11

A microecological conditioning composition is different from example 1 in that the humectant is a composition of glycerin and hyaluronic acid in a weight ratio of 1:1.

Example 12

A microecological conditioning composition is different from example 1 in that the solvent is a 1:1 by weight ratio of dipropylene glycol to dipropylene oxide.

Example 13

A microecological regulating composition is different from the embodiment 1 in that the raw material in the step (1) further contains 2-5% by weight of auxiliary agent, wherein the auxiliary agent is composed of silica, nano graphene and beeswax according to the weight ratio of 0.4:0.2:1, and the auxiliary agent is preferably 3% by weight of auxiliary agent when the microecological regulating composition is applied together with the raw materials of other components in the step (1).

Example 14

A microecological conditioning composition is different from example 13 in that the auxiliary agent consists of silica, nano graphene and beeswax in a weight ratio of 0.2:0.1:1.

Example 15

A microecological conditioning composition is different from example 13 in that the auxiliary agent consists of silica, nano graphene and beeswax in a weight ratio of 0.7:0.3:1.

Example 16

A microecological conditioning composition is different from example 13 in that the auxiliary agent consists of silica, nano graphene and beeswax in a weight ratio of 0.45:0.2:1.

Example 17

A microecological conditioning composition differs from example 13 in that the mass of silica is replaced by deionized water.

Example 18

A microecological conditioning composition differs from example 13 in that the nano graphene is replaced by deionized water in equal mass.

Example 19

A microecological conditioning composition differs from example 13 in that the mass of beeswax is replaced by deionized water.

Example 20

A microecological conditioning composition differs from example 13 in that the mass of silica, nanographene, etc. is replaced by deionized water.

Example 21

A microecological conditioning composition differs from example 13 in that the mass of silica, beeswax, etc. is replaced with deionized water.

Example 22

A microecological conditioning composition is different from example 13 in that the mass of nano graphene, beeswax, etc. is replaced with deionized water.

Example 23

The skin care product comprises the following raw materials in parts by weight as shown in table 1, and is prepared by the following steps:

(2) Mixing the phase A raw materials in the step (1), heating to 75-80 ℃, uniformly stirring, and stirring at 300r/min for 10min, mixing the phase B raw materials, heating to 75-80 ℃, uniformly stirring, stirring at 300r/min for 10min, adding the phase B into the phase A, uniformly stirring, and stirring at 300r/min for 5min;

(3) And (3) cooling the mixture of the phase A and the phase B in the step (2) to 35-45 ℃, adding the raw materials of the phase C and the phase D, stirring and mixing uniformly, and stirring for 15min at 300r/min to obtain the skin care product.

Note that in the above operation, both the a phase and the B phase are heated to 80 ℃, the mixture of the a phase and the B phase is preferably cooled to 40 ℃, and the microecological adjustment composition is obtained in preparation example 1.

Examples 24 to 25

A skin care product was different from example 23 in that the raw materials of the respective components and their respective weights are shown in Table 2.

TABLE 2 raw materials for each component and weight percentage in examples 23 to 25

Example 26

A skin care product was different from example 23 in that a microecological conditioning composition was obtained for example 11 and a portion of deionized water was removed to ensure the same percentage content of Lactobacillus fermentation lysate, lactobacillus/soymilk fermentation product filtrate and α -glucan oligosaccharides as in example 21.

Comparative example

Comparative example 1

A microecological conditioning composition differs from example 1 in that the equivalent mass of the Lactobacillus fermentation lysate is replaced by deionized water.

Comparative example 2

A microecological conditioning composition differs from example 1 in that the lactobacillus/soymilk fermentation product filtrate is replaced by deionized water in equal mass.

Comparative example 3

A microecological conditioning composition differs from example 1 in that the equivalent mass of alpha-glucan oligosaccharides is replaced by deionized water.

Comparative example 4

A microecological conditioning composition is different from example 1 in that the mass of Lactobacillus fermentation lysate, lactobacillus/soymilk fermentation product filtrate, and alpha-glucan oligosaccharides are replaced with deionized water.

Comparative example 5

A microecological conditioning composition is different from example 1 in that the quality of the compounded plant extract is replaced by deionized water.

Performance test samples the micro-ecological regulation compositions obtained in examples 1 to 22 were used as test samples 1 to 22, the skin care products obtained in examples 22 to 26 were used as test samples 22 to 26, and the micro-ecological regulation compositions obtained in comparative examples 1 to 5 were used as control samples 1 to 5.

The test method comprises the following steps:

The test person selects ① patients with acnes of 18-50 years old, 62 patients with acnes of 18-50 years old, the tested part extends outwards within 1cm from the skin where the acnes are located, the test requirement can be strictly complied with, and the volunteer can sign an informed consent. ② Exclusion criteria are pregnant or lactating women, those at risk of allergy or high sensitivity to the wash and care products, and those with immunodeficiency or other dermatological conditions at the test site. ③ Rejecting standard, namely actively applying for withdrawal, poor compliance, pregnancy, adverse reaction and losing visit of other special conditions in the experimental process, requiring withdrawal of the experimenter.

(1) The Propionibacterium acnes inhibition test comprises that 62 people are divided into two groups, each group of men and women is divided into 31 groups, each group of people takes 0.1mL tissue fluid of a tested part, 1mL physiological saline is added for dilution by 10 times, after mixing and inoculating to 5 agar culture mediums for culturing for 7d, colony numbers are counted, an average value is recorded as an initial value of each person, the initial values of each group of two people are added and averaged, each corresponding sample is recorded as A1, then 31 groups of people are respectively smeared with test samples 1-26 and control samples 1-5, each time is respectively used for eight points in the morning and evening, the smearing amount is 2mg/cm ², after continuous smearing for 15 days, colony number initial values corresponding to each person are measured by the same method, each group of two people are averaged after the initial values are added and each corresponding sample is recorded as A2, and the Propionibacterium acnes inhibition ratio (%) = (A1-A2)/A1 is recorded in table 3.

(2) The hBD-2 test comprises that hBD-2 is mainly expressed in epithelial tissues and is synthesized and secreted by keratinocytes, the hBD-2 is a cationic small molecular antibacterial peptide which protects skin from being affected by external pathogenic bacteria, the micro-ecology of the skin is closely related to the hBD-2 expression level, 31 groups of people, each group of people takes 0.1mL of tissue fluid of a tested part, 1mL of physiological saline is added for dilution by 10 times, the hBD-2 expression level is detected by an Elisa method and a fluorescence quantitative PCR detection method, the hBD-2 expression level of each group of two people is added and is averaged, each corresponding sample is recorded as B1, then 31 groups of people are respectively smeared with test samples 1-26 and control samples 1-5, the smearing amount is 2mg/cm ² at eight points in the morning and eight points in the evening, the corresponding hBD-2 expression level of each group of people is measured by the same method after continuous smearing for 17 days, each group of average value is taken and each sample is recorded as B2, and the change rate (%) of hBD-2 expression level is recorded in Table 3.

TABLE 3 test results for test samples 1-26 and control samples 1-5

It can be seen from the combination of example 1 and comparative examples 1 to 4 and the combination of Table 3 that the lactobacillus fermentation lysate, the lactobacillus/soybean milk fermentation product filtrate and the α -glucan oligosaccharides can play an excellent role in the combination and synergism, not only can inhibit and reduce propionibacterium acnes, effectively improve acne, but also can regulate skin micro-ecology, so that the skin micro-ecology is more excellent, and the expression level of hBD-2 is more outstanding. And any one of lactobacillus fermentation lysate, lactobacillus/soybean milk fermentation product filtrate and alpha-glucan oligosaccharides is singly used, compared with the situation that three raw materials are not used, the improvement can be brought about in the inhibition rate of propionibacterium acnes and the change rate of hBD-2 expression quantity, but the improvement effect is limited and far less than the excellent effect brought about by the compound use of the three raw materials.

As can be seen from the combination of examples 1, 4-6 and table 3, the obtained micro-ecological adjustment composition can maintain excellent and stable application effects by adopting the plant extracts composed of the flaxseed extract and the boldo leaf extract in a specific weight range ratio, wherein the effect is most excellent when the weight ratio of the flaxseed extract to the boldo leaf extract is 1.5:1. In combination with examples 7-8 and comparative example 5 and with Table 3, it can be seen that the combination of the flaxseed extract and the Boerdosia leaf extract can achieve synergistic effect. As can be seen from the combination of examples 9 to 10 and Table 3, when the ratio of the flaxseed extract to the Boerdosia leaf extract is lower than or higher than (1.3-1.9): 1, the application effect of the obtained microecological adjustment composition is obviously lost, which indicates that the ratio of the flaxseed extract to the Boerdosia leaf extract has higher importance. In combination with comparative examples 4 and 5 and with Table 3, it can be seen that the use of the compound plant extract alone without using the Lactobacillus fermentation lysate, the Lactobacillus/soymilk fermentation product filtrate and the α -glucan oligosaccharides gave far less effects than the use of the compound plant extract of example 1, indicating that the use of the compound plant extract has excellent effects of the cooperation between the Lactobacillus fermentation lysate, the Lactobacillus/soymilk fermentation product filtrate and the α -glucan oligosaccharides.

It can be seen from the combination of example 1, examples 13 to 22 and table 3 that the addition of the auxiliary agent composed of silica, nano graphene and beeswax according to a specific ratio can further improve the application effect of the microecological adjustment composition, and can significantly improve the propionibacterium acnes inhibition rate and hBD-2 expression change rate obtained by the test, wherein the improvement effect is better when the weight ratio of silica, nano graphene and beeswax is 0.4:0.2:1. Any one or any two of silica, nano graphene and beeswax are added for use, so that the lifting effect is limited, and the technical effect of 1+1>2 can be brought only when three raw materials are compounded by superposing the lifting effect.

The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.

Claims

1. A microecological regulation composition, characterized in that the microecological regulation composition comprises the following components in percentage by weight:

Lactobacillus fermentation lysate 0.1-5%;

Lactobacillus/soy milk fermentation product filtrate 0.05-2%;

α-Glucan oligosaccharide 0.1-10%;

Compound plant extract 0.2-20%;

Humectant 1-35%;

Solvent 0.1-10%;

The balance is deionized water;

The compound plant extract consists of flax seed extract and Bordeaux leaf extract, and the weight ratio of the flax seed extract to the Bordeaux leaf extract is (1.3-1.9):1.

2. The microecological regulation composition according to claim 1, characterized in that the weight ratio of the flaxseed extract to the Bordeaux leaf extract is 1.5:1.

3. The microecological regulation composition according to claim 1, characterized in that: the moisturizing agent is one or a combination of butylene glycol, glycerin, propylene glycol, salicylic acid and hyaluronic acid;

The solvent is one of 1,2-pentanediol and dipropylene glycol or a combination of the two.

4. The microecological regulation composition according to claim 1, characterized in that: 2-5% by weight of an auxiliary agent is also added to the components of the microecological regulation composition, and the auxiliary agent includes silica, nano-graphene and beeswax, and the weight ratio of silica, nano-graphene and beeswax is (0.2-0.7): (0.1-0.3): 1.

5. The microecological regulation composition according to claim 4, characterized in that the weight ratio of the silica, nano-graphene and beeswax is 0.4:0.2:1.

6. The method for preparing the microecological regulation composition according to claim 1, characterized in that it comprises the following steps:

(1) preparing raw materials including lactobacillus fermentation lysate, lactobacillus/soymilk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water according to the ratio;

(2) The lactobacillus fermentation lysate, lactobacillus/soy milk fermentation product filtrate, α-glucan oligosaccharide, compound plant extract, moisturizer, solvent and water in step (1) are stirred and mixed uniformly at a stirring temperature of 25-35° C., a stirring time of 5-20 min, and a stirring speed of 300-500 r/min to obtain a microecological regulation composition.

7. The use of the microecological regulation composition according to any one of claims 1 to 5, characterized in that it comprises a skin care product obtained by using the microecological regulation composition.

8. The use of the microecological regulation composition according to claim 7, characterized in that the skin care product comprises the following components in percentage by weight:

Poloxamer 407 5-30%;

1,3-Butanediol 1-5%;

Glycerol 0.5-10%;

Panthenol 0.1-1%;

β-Glucan 0.2-1%;

Niacinamide 1-10%;

Polyacrylate cross-linked polymer 0.2-3%;

Sodium hydroxide 0.01-0.5%;

Microecological regulation composition 0.1-10%;

p-Hydroxyacetophenone 0.5-0.8%;

1,2-Hexanediol 0.5-0.8%;

The balance was deionized water.

9. The method for preparing the skin care product according to claim 8, characterized in that it comprises the following steps:

(1) Prepare the raw materials of phase A, phase B, phase C and phase D according to the proportion, wherein phase A includes poloxamer 407, 1,3-butylene glycol and glycerol; phase B includes deionized water, panthenol, β-glucan, niacinamide and polyacrylate cross-linked polymer; phase C includes sodium hydroxide and microecological regulation composition; phase D includes p-hydroxyacetophenone and 1,2-hexanediol:

(2) Mix the raw materials of phase A in step (1), heat to 75-80°C, and stir evenly; mix the raw materials of phase B, heat to 75-80°C, and stir evenly; then add phase B to phase A and stir evenly;

(3) Cooling the mixture of phase A and phase B in step (2) to 35-45° C., adding the raw materials of phase C and phase D, stirring and mixing evenly, and obtaining a skin care product.