CN116806838A - Liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and preparation methods and applications thereof - Google Patents

Abstract

The invention relates to a liquid preparation containing 2-fluoro-N- (3-methoxyphenyl) -7H-purine-6-amine, and a preparation method and application thereof, belonging to the technical field of agricultural chemicals. The 2-fluoro-N- (3-methoxyphenyl) -7H-purine-6-amine-containing liquid preparation provided by the invention is prepared by compounding the 2-fluoro-N- (3-methoxyphenyl) -7H-purine-6-amine with different compounds, and simultaneously selecting a specific cosolvent, so that the liquid preparation has good lasting foamability, dilution stability, low-temperature stability and heat storage stability. The liquid preparation containing 2-fluoro-N- (3-methoxyphenyl) -7H-purine-6-amine has the functions of inhibiting decomposition of plant chlorophyll, nucleic acid and protein and protecting green and resisting aging; the fertilizer has various effects of transporting amino acid, auxin, inorganic salt and the like, and can be widely used for various stages from germination to harvest of agricultural, fruit tree and horticultural crops.

Description

Liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and preparation thereof Preparation methods and applications

Technical field

The invention relates to a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine and its preparation method and application, and belongs to the technical field of agricultural chemicals.

Background technique

According to the China Pesticide Industry Association, 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine (Anisiflupurin) is a newly announced fluorine-containing purine cytokinin by ISO. Its chemical The abstract registration number is 1089014-47-0; the international common name is anisiflupurin (P); the Chinese scientific name is 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine; the molecular formula is C ₁₂ H ₁₀ FN ₅ O; relative molecular mass is 259.239; melting point is 195°C; it is a 6-anilinopurine derivative; its chemical structure is as follows:

The original drug of Anisiflupurin is a white crystalline powder, hardly soluble in water, slightly soluble in ethanol, and stable in acids and alkali. Anisiflupurin is a 6-anilinopurine derivative; in agriculture, it can stimulate the growth of tobacco callus and maintain the chlorophyll content in isolated wheat leaves. It can also induce the synthesis of β-anthocyanin in amaranth cotyledons. Inhibits cytokinin oxidation/dehydrogenase induced by abiotic stress. In vitro test results show that anisiflupurin has low toxicity, and its toxicity to mammalian osteosarcoma cell line HOS, breast cancer cell line MCF-7 and mouse cell line fibroblast NIH-3T3 is comparable to that of kinetin. ) is equally toxic and has better anti-aging properties than kinetin on human fibroblasts after 80 passages. Therefore, Anisiflupurin has the advantages of low toxicity and good safety in use.

At present, many plant growth regulators often fail to achieve the desired effect when used alone. The main disadvantages of single-agent application are that the biological activity is relatively simple, the growth regulation effect is relatively low, the concentration is large, and it usually only works within a certain growth period of the crop. period before growth regulation can be exerted. Therefore, it is necessary to correctly and rationally select and mix different plant growth regulators in order to overcome or avoid side effects and achieve the expected application effects, that is, to achieve synergistic, additive or inducing effects and to avoid antagonism. However, there are currently no reports or registrations on preparations prepared by compounding Anisiflupurin with other compounds in China.

Therefore, there is an urgent need to develop a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine with good stability to better improve 2-fluoro-N Biological activity and growth regulation of -(3-methoxyphenyl)-7H-purin-6-amine.

Contents of the invention

The object of the present invention is to provide a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine, which can solve the problem of using 2-fluoro-N-(3- Methoxyphenyl)-7H-purin-6-amine has the problem of single activity and poor plant growth regulation effect.

The second object of the present invention is to provide a preparation method for a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine, which can solve the current problem of 2-fluoro- When used alone as a liquid preparation, N-(3-methoxyphenyl)-7H-purin-6-amine has the problem of single activity and poor plant growth regulation.

The third object of the present invention is to provide a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine as a plant growth regulator, which can solve the current problem of When 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine is used alone as a plant growth regulator, there is a problem of single activity and poor plant growth regulation effect.

In order to achieve the above objectives, the technical solution adopted by the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine of the present invention is:

A liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine, mainly composed of active ingredients, emulsifiers, solvents and co-solvents; the active ingredient consists of 2 -Fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and a compound; the compound is gibberellic acid GA3, gibberellic acid GA4+7, 24-epiyrene Lycosinolide, 28-homobrassinolide, acetate, ethephon or triacontanol; the co-solvent is dimethyl sulfoxide, N,N-dimethylformamide, N-methylpyrrolidone , lactic acid; the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in the liquid preparation is not greater than 10%; the mass fraction of the compound in the liquid preparation Not more than 30%.

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine of the present invention is prepared by adding 2-fluoro-N-(3-methoxyphenyl)-7H -Purine-6-amine is used in combination with different compounds, and specific co-solvents are selected at the same time, so that the liquid preparation can have good long-lasting foaming, dilution stability, low temperature stability and heat storage stability. The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine of the present invention has the effect of transporting amino acids, auxin, inorganic salts, etc. to the growth site, and can be widely used Used in all stages of agriculture, fruit trees and horticulture crops from germination to harvest.

Preferably, the compound is gibberellic acid GA3, gibberellic acid GA4+7, 24-epibrassinolide, 28-homobrassinolide, ampicillin or triacontanol, and the liquid preparation Stabilizers are also included, and the stabilizers are formic acid, acetic acid, lactic acid, 2,6-di-tert-butyl-p-cresol, and tert-butyl hydroquinone. The function of the stabilizer is to ensure that the dilution of the preparation remains uniform and free of precipitates during the dilution process with water; it is convenient for farmers to use and avoids clogging the atomizing nozzles of pesticide equipment.

Preferably, the compound is gibberellic acid GA3, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and gibberellic acid GA3 in the liquid preparation Independently no more than 0.9%;

Or the compound is gibberellic acid GA4+7, and the liquid preparation contains 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and gibberellic acid GA4+7. The mass fraction is independently not more than 1.8%;

Or the compound is 24-epibrassinolide, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.99%, the mass fraction of 24-epibrassinolide is not more than 0.01%;

Or the compound is 28-homobrassinolide, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 4.995% , the mass fraction of 28-homobrassinolide is not greater than 0.005%;

Or the compound is ethylamine, the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not more than 2%, and the ethylamine is The mass fraction of ester is not more than 8%;

Alternatively, the compound is ethephon, the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 0.5%, and ethephon is The mass fraction is not greater than 29.5%;

Or the compound is triacontanol, the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not more than 9.8%, and the three The mass fraction of decanol is not greater than 0.2%. In different liquid preparations, the content of active ingredients is too high, which will cause precipitation, crystallization, stratification and other phenomena when stored at room temperature.

Preferably, the compound is gibberellic acid GA3, and the stabilizer is formic acid and/or lactic acid; the mass fraction of the stabilizer in the liquid preparation is 0.5 to 2%;

Or the compound is gibberellic acid GA4+7, and the stabilizer is formic acid and/or lactic acid; the mass fraction of the stabilizer in the liquid preparation is 10 to 20%;

Or the compound is 24-epibrassinolide, and the stabilizer is formic acid and/or acetic acid; the mass fraction of the stabilizer in the liquid preparation is 3 to 6%;

Or the compound is 28-homobrassinolide, and the stabilizer is formic acid and/or acetic acid; the mass fraction of the stabilizer in the liquid preparation is 0.3 to 0.6%;

Alternatively, the compound is ethylenimine, and the stabilizer is 2,6-di-tert-butyl-p-cresol or tert-butyl hydroquinone; the mass fraction of the stabilizer in the liquid preparation is 1 to 3%;

Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The mass fraction of triacontanol in the liquid preparation is not greater than 0.1%, the stabilizer in the liquid preparation is acetic acid; the mass fraction of the stabilizer in the liquid preparation is 1 to 3%;

Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, the stabilizer in the liquid preparation is formic acid; the mass fraction of the stabilizer in the liquid preparation is 6 to 10% . The type and dosage of stabilizers have an important impact on the physical stability of liquid preparations. For different compounds, corresponding stabilizers and corresponding stabilizer dosages need to be used. If the type and dosage of stabilizers are changed, the liquid preparation will be The performance is not up to standard. When the amount of stabilizer is too much, the pH value of the preparation will be too low, causing decomposition of the active ingredients and other problems; when the amount of stabilizer is too small, precipitation will occur in the water dilution of the preparation, and the dilution stability will be unqualified.

Preferably, the compound is gibberellic acid GA3, and the emulsifier in the liquid preparation is octylphenol polyoxyethylene ether and/or fatty alcohol polyoxyethylene ether; the quality of the emulsifier in the liquid preparation is The score is 1 to 4%;

Or the compound is gibberellic acid GA4+7, and the emulsifier in the liquid preparation is octylphenol polyoxyethylene ether and/or fatty alcohol polyoxyethylene ether; the quality of the emulsifier in the liquid preparation The score is 2 to 4%;

Or the compound is 24-epibrassinolide, and the emulsifier in the liquid preparation is Tween 80; the mass fraction of the emulsifier in the liquid preparation is 3 to 7%;

Or the compound is 28-homobrassinolide, the emulsifier in the liquid preparation is polyethylene glycol 400; the mass fraction of the emulsifier in the liquid preparation is 4 to 8%;

Or the compound is ethyl ethyl, and the emulsifier in the liquid preparation is polyether; the mass fraction of the emulsifier in the liquid preparation is 0.5 to 2%;

Or the compound is ethephon, and the emulsifier in the liquid preparation is polyethylene glycol 400; the mass fraction of the emulsifier in the liquid preparation is 3 to 6%;

Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The emulsifier in the liquid preparation is fatty alcohol polyoxyethylene ether; the mass fraction of the emulsifier in the liquid preparation is 2 to 6%;

Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, the emulsifier in the liquid preparation is Tween 80; the mass fraction of the emulsifier in the liquid preparation is 3~ 7%. The function of the emulsifier is to reduce the interfacial tension of each component in the mixed system and form a stronger film on the surface of the droplets or form a double electrical layer on the surface of the droplets through the charge given by the emulsifier to prevent the droplets from aggregating with each other. , thereby maintaining a uniform state. For different compounds, corresponding emulsifiers and corresponding amounts of emulsifiers need to be used. When the amount of emulsifier is too much, it will cause: the viscosity of the preparation system is too high, which is not conducive to filling and metering in actual production; at the same time, low temperature is easy to There will be turbidity in the preparation; too much dosage and too strong permeability of the preparation will cause phytotoxicity to crops. When the amount of emulsifier is too small, it will cause: the droplets of the active ingredients of the preparation aggregate and flocculate, resulting in the unqualified phenomenon of crystal precipitation and precipitation.

Preferably, the compound is gibberellic acid GA3, and the co-solvent in the liquid preparation is dimethyl sulfoxide; the mass fraction of the co-solvent in the liquid preparation is 15 to 25%;

Or the compound is gibberellic acid GA4+7, and the co-solvent in the liquid preparation is N,N-dimethylformamide; the mass fraction of the co-solvent in the liquid preparation is 30 to 40%;

Or the compound is 24-epibrassinolide, and the co-solvent in the liquid preparation is N-methylpyrrolidone and/or dimethyl sulfoxide; the mass fraction of the co-solvent in the liquid preparation is 10~20%;

Or the compound is 28-homobrassinolide, the co-solvent in the liquid preparation is N-methylpyrrolidone and/or dimethyl sulfoxide; the mass fraction of the co-solvent in the liquid preparation is 20 ~40%;

Or the compound is ethyl acetate, and the co-solvent in the liquid preparation is N-methylpyrrolidone; the mass fraction of the co-solvent in the liquid preparation is 20 to 30%;

Or the compound is ethephon, the co-solvent in the liquid preparation is lactic acid; the mass fraction of the co-solvent in the liquid preparation is 30 to 40%;

Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The mass fraction of triacontanol in the liquid preparation is not greater than 0.1%, the co-solvent in the liquid preparation is dimethyl sulfoxide; the mass fraction of the co-solvent in the liquid preparation is 10 to 20%;

Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, the co-solvent in the liquid preparation is N-methylpyrrolidone; the mass fraction of the co-solvent in the liquid preparation is 30~40%. The role of co-solvent is to dissolve the active ingredients in liquid preparations, and can form complexes, associations or double salts with insoluble substances to increase the solubility of the drug in the solvent. Co-solvents can also increase the stability of preparations, improve physical properties, correct smell and taste, reduce irritation, improve absorption, and increase pharmacological effects. If the amount of co-solvent is too small, the active ingredients of the preparation will not be completely dissolved, and unstable phenomena such as crystal precipitation and precipitation will easily occur.

Preferably, the compound is gibberellic acid GA3, the solvent in the liquid preparation is an alcoholic solvent, and the alcoholic solvent is ethylene glycol and/or propylene glycol;

Or the compound is gibberellic acid GA4+7, the solvent in the liquid preparation is an alcoholic solvent, and the alcoholic solvent is ethylene glycol and/or propylene glycol;

Or the compound is 24-epibrassinolide, and the solvent in the liquid preparation is propylene glycol;

Or the compound is 28-homobrassinolide, and the solvent in the liquid preparation is N,N-dimethylformamide;

Or the compound is ethylcarboxylate, and the solvent in the liquid preparation is N,N-dimethylformamide;

Or the compound is ethephon, and the solvent in the liquid preparation is selected from one or any combination of water, ethylene glycol, and propylene glycol;

Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The mass fraction of triacontanol in the liquid preparation is not greater than 0.1%, and the solvent in the liquid preparation is propylene glycol;

Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, and the solvent in the liquid preparation is N,N-dimethylformamide. Solvents in liquid preparations have the following functions: ① Dilution: Solvents can dilute concentrated substances, making them easier to operate or handle. ②Dissolution: It can dissolve substances that are insoluble in water and turn them into soluble substances. ③Stabilizing effect: Solvents can stabilize certain substances so that they are not easily oxidized or decomposed. ④Change the physical properties of a substance: Solvents can change the physical properties of a substance, such as viscosity, surface tension, etc., making it easier to operate or handle. If the amount of solvent is too small, the solubility of the active ingredients of the preparation will be low and the dissolution will be incomplete, resulting in crystal precipitation.

Preferably, the liquid preparation is A composition, B composition, C composition, D composition, E composition, F composition or G composition;

The A composition mainly consists of active ingredients, stabilizers, emulsifiers, solvents and co-solvents; the active ingredient in the A composition consists of 2-fluoro-N-(3-methoxyphenyl)-7H- Composed of purine-6-amine and gibberellic acid GA3; the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and gibberellic acid GA3 in the A composition Independently, it is no more than 0.9%; the stabilizer in the A composition is formic acid and/or lactic acid; the mass fraction of the stabilizer in the A composition is 0.5 to 2%; the emulsification in the A composition The agent is octylphenol polyoxyethylene ether and/or fatty alcohol polyoxyethylene ether; the mass fraction of the emulsifier in the composition A is 1 to 4%; the solvent in the composition A is an alcohol solvent; The solvent in the A composition is ethylene glycol and/or propylene glycol; the co-solvent in the A composition is dimethyl sulfoxide; the mass fraction of the co-solvent in the A composition is 15 to 25%;

The B composition mainly consists of active ingredients, stabilizers, emulsifiers, solvents and co-solvents; the active ingredient in the B composition consists of 2-fluoro-N-(3-methoxyphenyl)-7H- Composed of purine-6-amine and gibberellic acid GA4+7; 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine and gibberellic acid GA4+ in the B composition The mass fraction of 7 is independently no more than 1.8%; the stabilizer in the B composition is formic acid and/or lactic acid; the mass fraction of the stabilizer in the B composition is 10 to 20%; the B combination The emulsifier in the composition is octylphenol polyoxyethylene ether and/or fatty alcohol polyoxyethylene ether; the mass fraction of the emulsifier in the B composition is 2 to 4%; the solvent in the B composition is Alcohol solvent; the solvent in the B composition is ethylene glycol and/or propylene glycol; the co-solvent in the B composition is N,N-dimethylformamide; the co-solvent in the B composition is The mass fraction is 30~40%;

The C composition mainly consists of active ingredients, stabilizers, emulsifiers, solvents and co-solvents; the active ingredient in the C composition consists of 2-fluoro-N-(3-methoxyphenyl)-7H- Purine-6-amine and 24-epibrassinolide; the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in the C composition is not Greater than 1.99%, the mass fraction of the 24-epibrassinolide is not greater than 0.01%; the stabilizer in the C composition is formic acid and/or acetic acid; the mass fraction of the stabilizer in the C composition is 3 to 6%; the emulsifier in the C composition is Tween 80; the mass fraction of the emulsifier in the C composition is 3 to 7%; the solvent in the C composition is propylene glycol; The co-solvent in the C composition is N-methylpyrrolidone and/or dimethyl sulfoxide; the mass fraction of the co-solvent in the C composition is 10 to 20%;

The D composition mainly consists of active ingredients, stabilizers, emulsifiers, solvents and co-solvents; the active ingredient in the D composition consists of 2-fluoro-N-(3-methoxyphenyl)-7H- Composed of purine-6-amine and 28-homobrassinolide; the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in the D composition is not greater than 4.995 %, the mass fraction of the 28-homobrassinolide is not greater than 0.005%; the stabilizer in the D composition is formic acid and/or acetic acid; the mass fraction of the stabilizer in the D composition is 0.3 to 0.6 %; the emulsifier in the D composition is polyethylene glycol 400; the mass fraction of the emulsifier in the D composition is 4 to 8%; the solvent in the D composition is N,N-di Methyl formamide; the co-solvent in the D composition is N-methylpyrrolidone and/or dimethyl sulfoxide; the mass fraction of the co-solvent in the D composition is 20 to 40%;

The E composition mainly consists of active ingredients, stabilizers, emulsifiers, solvents and co-solvents; the active ingredient in the E composition consists of 2-fluoro-N-(3-methoxyphenyl)-7H- Composed of purine-6-amine and ammonium ester; the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in the E composition is not more than 2%; so The mass fraction of ethyl ester in the E composition is not more than 8%; the stabilizer in the E composition is 2,6-di-tert-butyl-p-cresol and/or tert-butyl hydroquinone; so The mass fraction of the stabilizer in the E composition is 1 to 3%; the emulsifier in the E composition is polyether; the mass fraction of the emulsifier in the E composition is 0.5 to 2%; The solvent in the E composition is N,N-dimethylformamide; the co-solvent in the E composition is N-methylpyrrolidone; the mass fraction of the co-solvent in the E composition is 20 to 30% ; The polyether is an EO/PO block polyether;

The F composition mainly consists of active ingredients, emulsifiers, solvents and co-solvents; the active ingredient in the F composition consists of 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6 -Composed of amine and ethephon; the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in the F composition is not greater than 0.5%; the F composition The mass fraction of ethephon in the F composition is not more than 29.5%; the emulsifier in the F composition is polyethylene glycol 400; the mass fraction of the emulsifier in the F composition is 3 to 6%; the F combination The solvent in the composition is selected from one or any combination of water, ethylene glycol, and propylene glycol; the co-solvent in the F composition is lactic acid; the mass fraction of the co-solvent in the F composition is 30 to 40% ;

The G composition mainly consists of active ingredients, stabilizers, emulsifiers, solvents and co-solvents; the active ingredient in the G composition consists of 2-fluoro-N-(3-methoxyphenyl)-7H- Composed of purine-6-amine and triacontanol;

The mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the G composition is not greater than 1.9%, and the mass fraction of triacontanol in the G composition is The mass fraction is not more than 0.1%, the stabilizer in the G composition is acetic acid; the mass fraction of the stabilizer in the G composition is 1 to 3%; the emulsifier in the G composition is fatty alcohol poly Oxyethylene ether; the mass fraction of the emulsifier in the G composition is 2 to 6%; the solvent in the G composition is propylene glycol; the co-solvent in the G composition is dimethyl sulfoxide; The mass fraction of the co-solvent in the G composition is 10 to 20%;

Or the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the G composition is >1.9% and ≤9.8%, and the three The mass fraction of decanol is >0.1% and ≤0.2%, and the stabilizer in the G composition is formic acid; the mass fraction of the stabilizer in the G composition is 6 to 10%; The emulsifier is Tween 80; the mass fraction of the emulsifier in the G composition is 3 to 7%; the solvent in the G composition is N,N-dimethylformamide; the G composition The co-solvent in the G composition is N-methylpyrrolidone; the mass fraction of the co-solvent in the G composition is 30 to 40%.

The emulsifier in the E composition can be commercially available products, for example, the product model Ethylan NS-500LQ produced by Nanjing Jierun Technology Co., Ltd. This product is a non-ionic hydroxyl polyethylene oxide block copolymer, EO The molar ratio of /PO is 1:1.71, the pH is 6.5-7.5, and it has good emulsifying, wetting and dispersing functions.

The technical solution adopted in the preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine of the present invention is:

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine as described above includes the following steps: Mix the components of the formula evenly to obtain Liquid formulation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine of the present invention is simple to operate, and the prepared liquid preparation containing 2-fluoro-N-(3-methyl The liquid preparation of oxyphenyl)-7H-purin-6-amine has good long-lasting foaming properties, dilution stability, low temperature stability and heat storage stability.

Preferably, the method of uniform mixing includes the following steps: dissolving the active ingredients in each composition in a co-solvent, then adding other components and mixing.

The technical solution adopted for the application of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as a plant growth regulator of the present invention is:

Use of a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine as described above as a plant growth regulator.

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine of the present invention can be used as a plant growth regulator to effectively promote plant growth.

Use of a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine as described above in promoting plant growth.

Preferably, the liquid preparation is composition A and/or composition B, and the liquid preparation has the effect of promoting the growth of coleoptiles. Further preferably, the liquid preparation is composition A and/or composition B, and the liquid preparation has the effect of promoting the growth of oat coleoptiles.

Preferably, the liquid preparation is composition C and/or composition D. The liquid preparation has the effect of increasing plant height and/or increasing the content of nutrients in plant leaves. The nutrients are selected from chlorophyll, protein. and fats alone or in any combination. Further preferably, the liquid preparation is composition C and/or composition D, and the liquid preparation has the effect of increasing plant height and/or increasing the content of nutrient components in plant leaves, and the nutrient components are selected from the group consisting of chlorophyll, One or any combination of protein and fat. Preferably, the liquid preparation is composition C and/or composition D, and the food is peanuts.

Preferably, the liquid preparation is E composition and/or F composition, and the liquid preparation has the effect of increasing the plant fruit setting rate and/or improving the plant fruit quality. The plant fruit quality is overweight per fruit and/or fruit. Vitamin C content in . Further preferably, the liquid preparation is the E composition and/or the F composition, and the liquid preparation has the effect of increasing the apple fruit setting rate and/or improving the apple fruit quality, and the apple fruit quality is overweight per fruit and/or Vitamin C content in the fruit.

Preferably, the liquid preparation is G composition, and the liquid preparation has the effect of increasing the volume and/or weight of plant fruits. Preferably, the liquid preparation is G composition, and the liquid preparation has the effect of increasing plant fruit diameter. Further preferably, the liquid preparation is G composition, and the liquid preparation has the effect of increasing the longitudinal diameter and/or transverse diameter of mango fruit.

Detailed ways

The technical solution of the present invention will be further described below with reference to specific embodiments.

The pH value of the liquid preparations in Examples 1-8 was tested in accordance with the provisions of standard GB/T 1601;

The durable foaming property is tested in accordance with the provisions of the standard GB/T 28137. The foam volume at 1 minute is recorded during the test. When the foam volume is not greater than 40mL, the durable foaming property test result is qualified, otherwise it is unqualified;

The test method for dilution stability is as follows: Use a pipette to absorb 5 mL of sample (liquid preparation), place it in a 100 mL graduated cylinder, dilute it to 100 mL with standard hard water, shake well, and place the graduated cylinder in a constant temperature water bath of 30±2°C. Let it stand for 1 hour and observe the appearance of the diluent in the graduated cylinder. If there are solid particles precipitating in the diluent, the dilution stability test result is unqualified. If there are no solid particles precipitating in the diluent, the dilution stability test result is qualified; Standard hard water is prepared according to the regulations in standard GB/T 14825;

The low-temperature stability is tested according to the method of "emulsions and homogeneous liquid preparations" in the standard GB/T 19137. The specific method is as follows: put 100mL of liquid preparation into a beaker, and then cold store the beaker at 0±2℃ for 7 days , then take out the beaker and return it to room temperature. Observe whether there is any oil precipitated in the cup, and measure the volume of the precipitated oil. If no oil precipitates or the volume of the precipitated oil does not exceed 0.3mL, perform a low-temperature stability test. The result is qualified. If the volume of precipitated oil exceeds 0.3mL, the low temperature stability test result is unqualified;

The test method for thermal storage stability is as follows: measure 30 mL of sample (liquid preparation) and conduct the test according to the "liquid preparation" method in standard GB/T 19136. The specific method is as follows: Inject the sample into a clean ampoule bottle with a syringe , and seal it with a high-temperature flame, then place the closed ampoule in a constant temperature oven at 54±2°C for 14 days, take it out, return it to room temperature, and measure the mass of the sample. If the mass of the sample does not change, store it again within 24 hours. Determine the mass fraction of the active ingredient in the sample, the pH value and dilution stability of the sample, if the mass fraction of the active ingredient is not less than 95% of that before heat storage, and the pH value and dilution stability of the sample after heat storage The test results are all qualified, then the test results of thermal storage stability are qualified.

The mass fraction of the active ingredients was tested using a liquid chromatography-ultraviolet detector. The liquid chromatography instrument was an Agilent 1260 high performance liquid chromatograph, and the ultraviolet instrument was a detector with an adjustable ultraviolet wavelength; the chromatographic column was 150 mm × 4.6 mm (id ), C18 5μm packing; micro injector 25μL; chromatographic conditions: mobile phase is methanol + water + acetic acid = 40 + 60 + 0.1 (V/V/V), degassed and filtered by ultrasonic; flow rate is 1.0mL/min ;The column temperature is 25±2℃; the detection wavelength is 210nm; the injection volume is 5μL.

Example 1

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this embodiment is prepared from 2-fluoro-N-(3-methoxyphenyl)-7H -Purine-6-amine (Anisiflupurin), gibberellic acid, stabilizer, emulsifier, solvent and co-solvent composition; types of gibberellic acid, stabilizer, emulsifier, solvent and co-solvent and the mass fraction of each component such as As shown in Table 1.

Table 1 Types of gibberellic acid, stabilizers, emulsifiers, solvents and co-solvents in the liquid preparation of Example 1 and the mass fraction of each component

Material type composition Mass fraction (%) Active ingredients Anisiflupurin 0.9 Active ingredients Gibberellic acid GA3 0.9 co-solvent DMSO 20 stabilizer Formic acid 1 emulsifier Octylphenol polyoxyethylene ether 2 Solvent Ethylene glycol 75.2

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in this embodiment specifically includes the following steps: first use a diaphragm pump to pump the co-solvent into the reaction In the kettle, start stirring at a stirring speed of 80 rpm. Add Anisiflupurin and gibberellic acid while stirring. After the addition is completed, stir for another 30 minutes. There will be no visible solute particles in the reaction kettle and complete dissolution will be achieved. Then add stabilizer, emulsifier, continue stirring for 60 min, finally add the solvent, continue stirring for 30 min, and obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

In other embodiments, when preparing the liquid preparation, the stirring speed is controlled to 40-80 rpm; after adding Anisiflupurin and gibberellic acid, the stirring time is controlled to 30-60 min; after adding the stabilizer and emulsifier, the stirring time is controlled is 30-60min; after adding the solvent, control the stirring time to 30-60min.

The liquid preparation of this example was tested for Anisiflupurin and gibberellic acid content, pH value, long-lasting foaming property, dilution stability, low temperature stability and thermal storage stability. The results are shown in Table 2.

Table 2 Performance test results of the liquid formulation of Example 1

In order to examine the impact of different co-solvents on liquid preparations, add 20 g of co-solvents to the mixing device, accurately weigh 0.9 g of Anisiflupurin and 0.9 g of gibberellic acid GA3, and stir and dissolve for 30 minutes at a speed of 80 rpm. , observe the dissolution of Anisiflupurin and gibberellic acid GA3, and the results are shown in Table 3.

Table 3 Dissolution of Anisiflupurin and gibberellic acid GA3 by different co-solvents

It can be seen from Table 3 that when using the same quality of absolute ethanol, cyclohexanone, propylene glycol methyl ether acetate, N,N-dimethylformamide and dimethyl sulfoxide to dissolve the raw materials, only N,N-dimethyl Formamide and dimethyl sulfoxide have the best dissolving effect on Anisiflupurin and gibberellic acid GA3.

In order to examine the impact of stabilizers on liquid preparations, liquid preparations containing different stabilizers were prepared according to the preparation method of liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 1. The liquid preparations, the type of stabilizer and the dilution stability of each liquid preparation prepared are shown in Table 4.

Table 4 Types of stabilizers and dilution stability of each prepared liquid formulation

It can be seen from Table 4 that when formic acid is used as the stabilizer, the dilution stability of the preparation is qualified; when aluminum formate, magnesium formate, and citric acid are used as the stabilizer, when the dilution stability is tested, precipitation appears at the bottom of the liquid preparation, which is unqualified.

Test of dilution stability when the co-solvent in Table 4 is replaced from dimethyl sulfoxide to N,N-dimethylformamide or the emulsifier is replaced from octylphenol polyoxyethylene ether to Tween 80 The results were all unqualified.

Finally, the influence of the dosage of each component on the liquid preparation was examined. The specific method was as follows: liquid containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine according to Example 1 The preparation method of the preparation is to prepare a liquid preparation. The difference is that the mass fraction of each component is changed. The results show that when the mass fraction of Anisiflupurin is ≤0.9%, the mass fraction of gibberellic acid GA3 is ≤0.9%, and the mass fraction of the cosolvent is 15~ 25%, the mass fraction of stabilizer is 0.5-2%, the mass fraction of emulsifier is 1-4%, and the balance is solvent, the liquid preparations prepared are all clear and transparent liquids, and the pH value is 2-5. The test results of long-lasting foaming, dilution stability, low temperature stability and thermal storage stability are all qualified. When the mass fraction of a certain component in the liquid preparation is not within the above numerical range, one or more of the performance indicators of the prepared liquid preparation do not meet the requirements.

Example 2

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this embodiment is prepared from 2-fluoro-N-(3-methoxyphenyl)-7H -Purine-6-amine (Anisiflupurin), gibberellic acid, stabilizer, emulsifier, solvent and co-solvent composition; types of gibberellic acid, stabilizer, emulsifier, solvent and co-solvent and the mass fraction of each component such as As shown in Table 5.

Table 5 Types of gibberellic acid, stabilizers, emulsifiers, solvents and co-solvents in the liquid preparation of Example 2 and the mass fraction of each component

Material type composition Mass fraction (%) Active ingredients Anisiflupurin 1.8 Active ingredients Gibberellic acid GA4+7 1.8 co-solvent N,N-dimethylformamide 35 stabilizer lactic acid 15 emulsifier Fatty alcohol polyoxyethylene ether 3 Solvent propylene glycol 43.4

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in this embodiment specifically includes the following steps: first use a diaphragm pump to pump the co-solvent into the reaction In the kettle, start stirring at a stirring speed of 40 rpm. Add Anisiflupurin and gibberellic acid while stirring. After the addition is completed, stir for another 60 minutes. There will be no visible solute particles in the reaction kettle and complete dissolution will be achieved. Then add stabilizer, emulsifier, continue stirring for 30 minutes, finally add the solvent, continue stirring for 60 minutes, and obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

In other embodiments, when preparing the liquid preparation, the stirring speed is controlled to 40-80 rpm; after adding Anisiflupurin and gibberellic acid, the stirring time is controlled to 30-60 min; after adding the stabilizer and emulsifier, the stirring time is controlled is 30-60min; after adding the solvent, control the stirring time to 30-60min.

The liquid preparation of this example was tested for Anisiflupurin and gibberellic acid content, pH value, long-lasting foaming property, dilution stability, low temperature stability and thermal storage stability. The results are shown in Table 6.

Table 6 Performance test results of the liquid formulation of Example 2

project index Test results Result judgment Mass fraction of Anisiflupurin, % 1.8±0.27 1.87 qualified Mass fraction of gibberellic acid GA4+7, % 1.8±0.27 1.82 qualified pH value 2-5 3.91 qualified Durable foaming (after 1 minute), mL≤ 40 20 qualified dilution stability qualified qualified qualified Low temperature stability qualified qualified qualified Thermal storage stability qualified qualified qualified

In order to examine the impact of different co-solvents on liquid preparations, add 35 g of co-solvents to the mixing device, accurately weigh 1.8 g of Anisiflupurin and 1.8 g of gibberellic acid GA4+7, and stir at a speed of 40 rpm. Dissolve for 60 minutes and observe the dissolution of Anisiflupurin and gibberellic acid GA4+7. The results are shown in Table 7.

Table 7 Dissolution of Anisiflupurin and gibberellic acid GA4+7 by different co-solvents

It can be seen from Table 7 that when the same mass of trimethyl phosphate, monoethanolamine, methyl isobutyl ketone, N-methylpyrrolidone and N,N-dimethylformamide is used to dissolve the raw materials, only N-methylpyrrolidone and N,N-dimethylformamide has the best dissolving effect on Anisiflupurin and gibberellic acid GA4+7.

In order to examine the impact of stabilizers on liquid preparations, liquid preparations containing different stabilizers were prepared according to the preparation method of liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 2. The liquid preparations, the types of stabilizers and the dilution stability of each liquid preparation prepared are shown in Table 8.

Table 8 Types of stabilizers and dilution stability of each liquid formulation produced

It can be seen from Table 8 that when lactic acid is used as the stabilizer, the dilution stability of the preparation is qualified; when aluminum sulfate, iron sulfate, and malic acid are used as the stabilizer, when the dilution stability is tested, precipitation appears at the bottom of the liquid preparation, which is unqualified.

When the co-solvent in Table 8 is replaced from N,N-dimethylformamide to N-methylpyrrolidone or the emulsifier is replaced from fatty alcohol polyoxyethylene ether to Tween 80, the test results of dilution stability All are unqualified.

Finally, the influence of the dosage of each component on the liquid preparation was examined. The specific method was as follows: liquid containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine according to Example 2 The preparation method of the preparation is to prepare a liquid preparation. The difference is that the mass fraction of each component is changed. The results show that when the mass fraction of Anisiflupurin is ≤1.8%, the mass fraction of gibberellic acid GA4+7 is ≤1.8%, the mass fraction of the cosolvent is When the mass fraction of stabilizer is 30 to 40%, the mass fraction of stabilizer is 10 to 20%, the mass fraction of emulsifier is 2 to 4%, and the balance is solvent, the liquid preparations prepared are all clear and transparent liquids with a pH value of 2 to 5. The test results of long-lasting foaming, dilution stability, low temperature stability and heat storage stability are all qualified. When the mass fraction of a certain component in the liquid preparation is not within the above numerical range, one or more of the performance indicators of the prepared liquid preparation do not meet the requirements.

Example 3

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this embodiment is prepared from 2-fluoro-N-(3-methoxyphenyl)-7H - Purine-6-amine (Anisiflupurin), brassinolide, stabilizer, emulsifier, solvent and co-solvent composition; types and components of brassinolide, stabilizer, emulsifier, solvent and co-solvent The quality scores are shown in Table 9.

Table 9 Types of brassinolides, stabilizers, emulsifiers, solvents and co-solvents in the liquid preparation of Example 3 and the mass fraction of each component

Material type composition Mass fraction (%) Active ingredients Anisiflupurin 1.99 Active ingredients 24-Epibrassinolide 0.01 co-solvent N-methylpyrrolidone 15 stabilizer glacial acetic acid 5 emulsifier Tween 80 5 Solvent propylene glycol 73

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in this embodiment specifically includes the following steps: first use a diaphragm pump to pump the co-solvent into the reaction In the kettle, start stirring at a stirring speed of 80 rpm. Add Anisiflupurin and brassinolide while stirring. After the addition is completed, stir for another 30 minutes. There will be no visible solute particles in the reaction kettle and complete dissolution will be achieved; then add in order to stabilize agent and emulsifier, continue stirring for 60 minutes, finally add solvent, continue stirring for 30 minutes, and obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

In other embodiments, when preparing liquid preparations, the stirring speed is controlled to 40-80 rpm; after adding Anisiflupurin and brassinolide, the stirring time is controlled to 30-60 min; after adding stabilizers and emulsifiers, controlling The stirring time is 30-60min; after adding the solvent, control the stirring time to 30-60min.

The liquid preparation of this example was tested for the content of Anisiflupurin and brassinolide, pH value, long-lasting foaming property, dilution stability, low temperature stability and heat storage stability. The results are shown in Table 10.

Table 10 Performance test results of the liquid formulation of Example 3

project index Test results Result judgment Mass fraction of Anisiflupurin, % 1.99±0.2985 2.01 qualified 24-Mass fraction of epibrassinolide, % 0.01±0.0015 0.0101 qualified pH value 2-5 4.37 qualified Durable foaming (after 1 minute), mL≤ 40 16 qualified dilution stability qualified qualified qualified Low temperature stability qualified qualified qualified Thermal storage stability qualified qualified qualified

In order to examine the impact of different co-solvents on liquid preparations, add 15 g of co-solvents to the mixing device, accurately weigh 1.99 g of Anisiflupurin and 0.01 g of 24-epibrassinolide at a speed of 80 rpm. , stir and dissolve for 30 minutes, and observe the dissolution of Anisiflupurin and 24-epibrassinolide. The results are shown in Table 11.

Table 11 Dissolution of Anisiflupurin and 24-epibrassinolide by different cosolvents

It can be seen from Table 11 that when the same quality of triethanolamine, n-butyl ether, ethyl acetate, ethylene glycol and N-methylpyrrolidone are used to dissolve the raw materials, only ethylene glycol and N-methylpyrrolidone have significant effects on Anisiflupurin and 24-epibrassinoline. Lactone has the best dissolving effect.

In order to examine the impact of stabilizers on liquid preparations, liquid preparations containing different stabilizers were prepared according to the preparation method of liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 3. The liquid preparations, the types of stabilizers and the dilution stability of each liquid preparation prepared are shown in Table 12.

Table 12 Types of stabilizers and dilution stability of each liquid formulation produced

It can be seen from Table 12 that when glacial acetic acid is used as the stabilizer, the dilution stability of the preparation is qualified; when ammonia water, 0.1 mol/L NaOH aqueous solution, and tartaric acid are used as the stabilizer, when the dilution stability is tested, precipitation appears at the bottom of the liquid preparation , unqualified.

When the co-solvent in Table 12 is replaced from N-methylpyrrolidone to ethylene glycol or the emulsifier is replaced from Tween 80 to Span 60, the dilution stability test results are unqualified.

Finally, the influence of the dosage of each component on the liquid preparation was examined. The specific method is as follows: liquid containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine according to Example 3 The preparation method of the preparation is to prepare a liquid preparation. The difference is that the mass fraction of each component is changed. The results show that when the mass fraction of Anisiflupurin is ≤1.99%, the mass fraction of 24-homobrassinolide is ≤0.01%, and the mass fraction of the cosolvent is When the mass fraction of the stabilizer is 3 to 6%, the mass fraction of the emulsifier is 3 to 7%, and the balance is solvent, the liquid preparations prepared are all clear and transparent liquids with a pH value of 2 to 20%. 5. The test results of long-lasting foaming, dilution stability, low temperature stability and heat storage stability are all qualified. When the mass fraction of a certain component in the liquid preparation is not within the above numerical range, one or more of the performance indicators of the prepared liquid preparation do not meet the requirements.

Example 4

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this embodiment is prepared from 2-fluoro-N-(3-methoxyphenyl)-7H - Purine-6-amine (Anisiflupurin), brassinolide, stabilizer, emulsifier, solvent and co-solvent composition; types and components of brassinolide, stabilizer, emulsifier, solvent and co-solvent The quality scores are shown in Table 13.

Table 13 Types of brassinolides, stabilizers, emulsifiers, solvents and co-solvents in the liquid preparation of Example 4 and the mass fraction of each component

Material type composition Mass fraction(%) Active ingredients Anisiflupurin 4.995 Active ingredients 28-Homobrassinolide 0.005 co-solvent DMSO 30 stabilizer Formic acid 0.5 emulsifier Polyethylene glycol 400 6 Solvent N,N-dimethylformamide 58.5

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in this embodiment specifically includes the following steps: first use a diaphragm pump to pump the co-solvent into the reaction In the kettle, start stirring at a stirring speed of 40 rpm. Add Anisiflupurin and brassinolide while stirring. After the addition is completed, stir for another 60 minutes. There will be no visible solute particles in the reaction kettle and complete dissolution will be achieved. Then add in order to stabilize the reaction. agent and emulsifier, continue stirring for 30 minutes, finally add solvent, continue stirring for 60 minutes, and obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

In other embodiments, when preparing liquid preparations, the stirring speed is controlled to 40-80 rpm; after adding Anisiflupurin and brassinolide, the stirring time is controlled to 30-60 min; after adding stabilizers and emulsifiers, controlling The stirring time is 30-60min; after adding the solvent, control the stirring time to 30-60min.

The liquid preparation of this example was tested for the content of Anisiflupurin and brassinolide, pH value, long-lasting foaming property, dilution stability, low temperature stability and heat storage stability. The results are shown in Table 14.

Table 14 Performance test results of the liquid formulation of Example 4

project index Test results Result judgment Mass fraction of Anisiflupurin, % 4.995±0.4995 4.990 qualified 28-Mass fraction of high brassinolide, % 0.005±0.00075 0.0049 qualified pH value 2-5 3.91 qualified Durable foaming (after 1 minute), mL≤ 40 13 qualified dilution stability qualified qualified qualified Low temperature stability qualified qualified qualified Thermal storage stability qualified qualified qualified

In order to examine the impact of different co-solvents on liquid preparations, add 30 g of co-solvents to the mixing device, accurately weigh 4.995 g of Anisiflupurin and 0.005 g of 28-homobrastisolide, and stir at a speed of 40 rpm. Dissolve for 60 minutes and observe the dissolution of Anisiflupurin and 28-homobrassinolide. The results are shown in Table 15.

Table 15 Dissolution of Anisiflupurin and 28-Homobrassinolide by different co-solvents

It can be seen from Table 15 that when the same quality of absolute ethanol, n-butanol, ethyl acetate, propylene glycol and dimethyl sulfoxide are used to dissolve the raw materials, only propylene glycol and dimethyl sulfoxide have an effective effect on Anisiflupurin and 28-homobrassinolide. Dissolving effect is the best.

In order to examine the impact of stabilizers on liquid preparations, liquid preparations containing different stabilizers were prepared according to the preparation method of liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 4. The liquid preparations, the type of stabilizer and the dilution stability of each liquid preparation prepared are shown in Table 16.

Table 16 Types of stabilizers and dilution stability of each liquid formulation produced

It can be seen from Table 16 that when formic acid is used as the stabilizer, the dilution stability of the preparation is qualified; when aluminum formate, magnesium formate, and propionic acid are used as the stabilizer, when the dilution stability is tested, precipitation appears at the bottom of the liquid preparation, which is unqualified.

When the co-solvent in Table 16 is replaced from dimethyl sulfoxide to propylene glycol or the emulsifier is replaced from polyethylene glycol 400 to Span 80, the dilution stability test results are unqualified.

Finally, the impact of the dosage of each component on the liquid preparation was examined. The specific method was as follows: Liquid containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine according to Example 4 The preparation method of the preparation is to prepare a liquid preparation. The difference is that the mass fraction of each component is changed. The results show that when the mass fraction of Anisiflupurin is ≤4.995%, the mass fraction of 28-homobrassinolide is ≤0.005%, and the mass fraction of the cosolvent is 20-40%, the mass fraction of stabilizer is 0.3-0.6%, the mass fraction of emulsifier is 4-8%, and the balance is solvent, the liquid preparations prepared are all clear and transparent liquids, and the pH value is 2- 5. The test results of long-lasting foaming, dilution stability, low temperature stability and heat storage stability are all qualified. When the mass fraction of a certain component in the liquid preparation is not within the above numerical range, one or more of the performance indicators of the prepared liquid preparation do not meet the requirements.

Example 5

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this embodiment is prepared from 2-fluoro-N-(3-methoxyphenyl)-7H -Purine-6-amine (Anisiflupurin), ethanol, stabilizer, emulsifier, solvent and co-solvent; the types of stabilizer, emulsifier, solvent and co-solvent and the mass fraction of each component are shown in Table 17 . The manufacturer of the EO/PO block polyether in Table 17 is Nanjing Jierun Technology Co., Ltd., and the product model is Ethylan NS-500LQ. The molar ratio of EO/PO in the EO/PO block polyether is 1:1.71.

Table 17 Types of stabilizers, emulsifiers, solvents and co-solvents in the liquid preparation of Example 5 and the mass fraction of each component

Material type composition Mass fraction (%) Active ingredients Anisiflupurin 2 Active ingredients ethanol 8 co-solvent N-methylpyrrolidone 15 stabilizer BHT (2,6-di-tert-butyl-p-cresol) 2 emulsifier EO/PO block polyether 1 Solvent N,N-dimethylformamide 72

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in this embodiment specifically includes the following steps: first use a diaphragm pump to pump the co-solvent into the reaction In the kettle, start stirring at a stirring speed of 80 rpm. While stirring, add Anisiflupurin and ampicillin. After the addition is completed, stir for another 30 minutes. There will be no visible solute particles in the reaction kettle and complete dissolution will be achieved. Then add stabilizer, emulsifier, continue stirring for 60 minutes, finally add the solvent, continue stirring for 30 minutes, and obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

In other embodiments, when preparing liquid preparations, the stirring speed is controlled to 40-80 rpm; after adding Anisiflupurin and ethanol, the stirring time is controlled to 30-60 min; after adding stabilizers and emulsifiers, the stirring time is controlled is 30-60min; after adding the solvent, control the stirring time to 30-60min.

The liquid preparation of this example was tested for Anisiflupurin and ethanol content, pH value, long-lasting foaming property, dilution stability, low temperature stability and thermal storage stability. The results are shown in Table 18.

Table 18 Performance test results of the liquid formulation of Example 5

project index Test results Result judgment Mass fraction of Anisiflupurin, % 2.0±0.3 2.1 qualified Mass fraction of ethylamine, % 8±0.8 7.9 qualified pH value 5.0-8.0 7.52 qualified Durable foaming (after 1 minute), mL≤ 40 18 qualified dilution stability qualified qualified qualified Low temperature stability qualified qualified qualified Thermal storage stability qualified qualified qualified

In order to examine the impact of different co-solvents on liquid preparations, add 15 g of co-solvents to the mixing device, accurately weigh 2.00 g of Anisiflupurin and 8.00 g of ethyl glycosides, and stir and dissolve for 30 minutes at a speed of 80 rpm. Observe the dissolution of Anisiflupurin and ethanol. The results are shown in Table 19.

Table 19 The dissolution of Anisiflupurin and ethanol by different cosolvents

It can be seen from Table 19 that when the same quality of trimethyl phosphate, monoethanolamine, methyl isobutyl ketone, ethylene glycol and N-methylpyrrolidone are used to dissolve the raw materials, only ethylene glycol and N-methylpyrrolidone have significant effects on Anisiflupurin and amines. Ester dissolves best.

In order to examine the impact of stabilizers on liquid preparations, liquid preparations containing different stabilizers were prepared according to the preparation method of liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 5. The liquid preparations, the type of stabilizer and the dilution stability of each liquid preparation prepared are shown in Table 20. BHT in Table 20 represents 2,6-di-tert-butyl-p-cresol, TBHQ represents tert-butylhydroquinone, the manufacturer of EO/PO block polyether is Nanjing Jierun Technology Co., Ltd., and the product model is Ethylan NS-500LQ.

Table 20 Types of stabilizers and dilution stability of each liquid formulation produced

It can be seen from Table 20 that when BHT or TBHQ is used as the stabilizer, the dilution stability of the preparation is qualified; when iron sulfate, ammonia water, and tea polyphenols are used as the stabilizer, when the dilution stability is tested, precipitation appears at the bottom of the liquid preparation. qualified.

When the co-solvent in Table 20 is replaced from N-methylpyrrolidone to ethylene glycol or the emulsifier is replaced from EO/PO block polyether to Tween 80, the test results for dilution stability are all unqualified.

Finally, the impact of the dosage of each component on the liquid preparation was examined. The specific method was as follows: Liquid containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine according to Example 5 The preparation method of the preparation is to prepare a liquid preparation. The difference is that the mass fraction of each component is changed. The results show that when the mass fraction of Anisiflupurin is ≤ 2%, the mass fraction of ethanol is ≤ 8%, and the mass fraction of the cosolvent is 20 to 30 %, the mass fraction of stabilizer is 1 to 3%, the mass fraction of emulsifier is 0.5 to 2%, and the balance is solvent, the liquid preparations prepared are all clear and transparent liquids with a pH value of 2 to 5 and are long-lasting. The test results of foaming property, dilution stability, low temperature stability and thermal storage stability are all qualified. When the mass fraction of a certain component in the liquid preparation is not within the above numerical range, one or more of the performance indicators of the prepared liquid preparation do not meet the requirements.

Example 6

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this embodiment is composed of the following mass fraction components: 2-fluoro-N-(3 -Methoxyphenyl)-7H-purin-6-amine (Anisiflupurin), ethephon, emulsifier, solvent and co-solvent; the types of emulsifier, solvent and co-solvent and the mass fraction of each component are shown in Table 21 shown.

Table 21 Types of emulsifiers, solvents and co-solvents in the liquid preparation of Example 6 and the mass fraction of each component

Material type composition Mass fraction (%) Active ingredients Anisiflupurin 0.5 Active ingredients ethephon 29.5 co-solvent lactic acid 35 emulsifier Polyethylene glycol 400 5 Solvent Deionized water 30

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in this embodiment specifically includes the following steps: first use a diaphragm pump to pump the co-solvent into the reaction In the kettle, start stirring at a stirring speed of 80 rpm. Add Anisiflupurin and Ethephon while stirring. After the addition is completed, stir for another 30 minutes. There will be no visible solute particles in the reaction kettle and complete dissolution is achieved. Add emulsifier in turn and continue. Stir for 60 minutes, finally add the solvent, and continue stirring for 30 minutes to obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

In other embodiments, when preparing the liquid preparation, the stirring speed is controlled to 40-80 rpm; after adding Anisiflupurin and ethephon, the stirring time is controlled to 30-60min; after the emulsifier is added, the stirring time is controlled to 30-60min ; After adding the solvent, control the stirring time to 30-60 minutes.

The liquid preparation of this example was tested for Anisiflupurin, ethephon content, pH value, persistent foaming property, dilution stability, low temperature stability and thermal storage stability. The results are shown in Table 22.

Table 22 Performance test results of the liquid formulation of Example 6

project index Test results Result judgment Mass fraction of Anisiflupurin, % 0.5±0.075 0.506 qualified Mass fraction of ethephon, % 29.5±1.475 29.8 qualified pH value 2.0-5.0 2.38 qualified Persistent foaming (after 1 minute), mL≤ 40 10 qualified dilution stability qualified qualified qualified Low temperature stability qualified qualified qualified Thermal storage stability qualified qualified qualified

In order to examine the influence of different cosolvents on liquid preparations, add 35g of cosolvents to the mixing device, accurately weigh 0.5g of Anisiflupurin and 29.5g of ethephon, stir and dissolve for 30 minutes at a speed of 80 rpm, and observe The results of the dissolution of Anisiflupurin and ethephon are shown in Table 23.

Table 23 Dissolution of Anisiflupurin and Ethephon by different co-solvents

It can be seen from Table 23 that when the same quality of absolute ethanol, n-butanol, ethyl acetate, propionic acid and lactic acid are used to dissolve the raw materials, only propionic acid and lactic acid have the best dissolving effect on Anisiflupurin and ethephon.

In order to examine the influence of solvents on liquid preparations, liquids containing different solvents were prepared according to the preparation method of liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 6. The formulation, type of solvent, and dilution stability of each liquid formulation produced are shown in Table 24.

Table 24 Types of solvents and dilution stability of each prepared liquid formulation

Material type composition Mass fraction (%) Mass fraction (%) Mass fraction (%) Active ingredients Anisiflupurin 0.5 0.5 0.5 Active ingredients ethephon 29.5 29.5 29.5 co-solvent lactic acid 35 35 35 emulsifier Polyethylene glycol 400 5 5 5 Solvent Ethylene glycol 30 - - Solvent propylene glycol - 30 - Solvent Deionized water - - 30 dilution stability - qualified qualified qualified

It can be seen from Table 24 that when ethylene glycol, propylene glycol, and deionized water are used as solvents, the dilution stability of the preparations is qualified; however, the cost of deionized water is lower.

When the cosolvent in Table 24 is replaced from lactic acid to propionic acid or the emulsifier is replaced from polyethylene glycol 400 to Tween 80, the test results for dilution stability are all unqualified.

Finally, the influence of the dosage of each component on the liquid preparation was examined. The specific method is as follows: liquid containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine according to Example 6 The preparation method of the preparation is to prepare a liquid preparation. The difference is that the mass fraction of each component is changed. The results show that when the mass fraction of Anisiflupurin is ≤0.5%, the mass fraction of ethephon is ≤29.5%, and the mass fraction of the cosolvent is 30 to 40%. , when the mass fraction of emulsifier is 3 to 6%, and the balance is solvent, the liquid preparations prepared are all clear and transparent liquids, with a pH value of 2 to 5, with long-lasting foaming properties, dilution stability, low temperature stability and The test results of thermal storage stability are all qualified. When the mass fraction of a certain component in the liquid preparation is not within the above numerical range, one or more of the performance indicators of the prepared liquid preparation do not meet the requirements.

Example 7

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this embodiment is prepared from 2-fluoro-N-(3-methoxyphenyl)-7H - Purine-6-amine (Anisiflupurin), triacontanol, stabilizer, emulsifier, solvent and co-solvent; the types of stabilizer, emulsifier, solvent and co-solvent and the mass fraction of each component are shown in Table 25 Show.

Table 25 Types of stabilizers, emulsifiers, solvents and co-solvents in the liquid preparation of Example 7 and the mass fraction of each component

Material type composition Mass fraction (%) Active ingredients Anisiflupurin 1.9 Active ingredients Triacontanol 0.1 co-solvent DMSO 15 stabilizer glacial acetic acid 2 emulsifier Fatty alcohol polyoxyethylene ether 4 Solvent propylene glycol 77

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in this embodiment specifically includes the following steps: first use a diaphragm pump to pump the co-solvent into the reaction In the kettle, start stirring at a stirring speed of 80 rpm. Add Anisiflupurin and triacontanol while stirring. After the addition is completed, stir for another 30 minutes. There will be no visible solute particles in the reaction kettle and complete dissolution will be achieved. Then add the stabilizer in turn. , emulsifier, continue stirring for 60 minutes, finally add the solvent, continue stirring for 30 minutes, and obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

In other embodiments, when preparing liquid preparations, control the stirring speed to 40-80 rpm; after adding Anisiflupurin and triacontanol, control the stirring time to 30-60 min; after adding stabilizers and emulsifiers, control stirring The time is 30-60min; after adding the solvent, control the stirring time to 30-60min.

The liquid preparation of this example was tested for Anisiflupurin and triacontanol content, pH value, long-lasting foaming property, dilution stability, low temperature stability and thermal storage stability. The results are shown in Table 26.

Table 26 Performance test results of the liquid formulation of Example 7

project index Test results Result judgment Mass fraction of Anisiflupurin, % 1.9±0.285 1.931 qualified Mass fraction of triacontanol, % 0.1±0.015 0.105 qualified pH value 2.0-5.0 3.68 qualified Durable foaming (after 1 minute), mL≤ 40 twenty four qualified dilution stability qualified qualified qualified Low temperature stability qualified qualified qualified Thermal storage stability qualified qualified qualified

In order to examine the impact of different co-solvents on liquid preparations, add 15 g of co-solvents to the mixing device, accurately weigh 1.9 g of Anisiflupurin and 0.1 g of triacontanol, and stir and dissolve for 30 minutes at a speed of 80 rpm. , observe the dissolution of Anisiflupurin and triacontanol, and the results are shown in Table 27.

Table 27 Dissolution of Anisiflupurin and triacontanol by different cosolvents

It can be seen from Table 27 that when the same quality of trimethyl phosphate, monoethanolamine, methyl isobutyl ketone, ethylene glycol and dimethyl sulfoxide are used to dissolve the raw materials, only ethylene glycol and dimethyl sulfoxide have significant effects on Anisiflupurin, 30 Alkanols have the best dissolving effect.

In order to examine the impact of stabilizers on liquid preparations, liquid preparations containing different stabilizers were prepared according to the preparation method of liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 7. The liquid preparations, the type of stabilizer and the dilution stability of each liquid preparation prepared are shown in Table 28.

Table 28 Types of stabilizers and dilution stability of each liquid formulation produced

It can be seen from Table 28 that when glacial acetic acid is used as the stabilizer, the dilution stability of the preparation is qualified; when iron sulfate, ammonia water, and lactic acid are used as the stabilizer, when the dilution stability is tested, precipitation appears at the bottom of the liquid preparation, which is unqualified.

When the cosolvent in Table 28 is replaced from dimethyl sulfoxide to ethylene glycol or the emulsifier is replaced from fatty alcohol polyoxyethylene ether to octylphenol polyoxyethylene ether, the test results of dilution stability are all Unqualified.

Finally, the influence of the dosage of each component on the liquid preparation was examined. The specific method is as follows: liquid containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine according to Example 7 The preparation method of the preparation is to prepare a liquid preparation. The difference is that the mass fraction of each component is changed. The results show that when the mass fraction of Anisiflupurin is ≤1.9%, the mass fraction of triacontanol is ≤0.1%, and the mass fraction of the cosolvent is 10~ 20%, the mass fraction of stabilizer is 1 to 3%, the mass fraction of emulsifier is 2 to 6%, and the balance is solvent, the liquid preparations prepared are all clear and transparent liquids, and the pH value is 2 to 5. The test results of long-lasting foaming, dilution stability, low temperature stability and thermal storage stability are all qualified. When the mass fraction of a certain component in the liquid preparation is not within the above numerical range, one or more of the performance indicators of the prepared liquid preparation do not meet the requirements.

Example 8

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this embodiment is composed of the following mass fraction components: 2-fluoro-N-(3 -Methoxyphenyl)-7H-purin-6-amine (Anisiflupurin), triacontanol, stabilizer, emulsifier, solvent and co-solvent composition; types of stabilizers, emulsifiers, solvents and co-solvents and their respective The mass fractions of the components are shown in Table 29.

Table 29 Types of stabilizers, emulsifiers, solvents and co-solvents in the liquid preparation of Example 8 and the mass fraction of each component

Material type composition Mass fraction (%) Active ingredients Anisiflupurin 9.8 Active ingredients Triacontanol 0.2 co-solvent N-methylpyrrolidone 35 stabilizer Formic acid 8 emulsifier Tween 80 5 Solvent N,N-dimethylformamide 42

The preparation method of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in this embodiment specifically includes the following steps: first use a diaphragm pump to pump the co-solvent into the reaction In the kettle, start stirring at a stirring speed of 40 rpm. Add Anisiflupurin and triacontanol while stirring. After the addition is complete, stir for another 60 minutes. There will be no visible solute particles in the reaction kettle and complete dissolution will be achieved. Then add the stabilizer in turn. , emulsifier, continue stirring for 30 minutes, finally add the solvent, continue stirring for 60 minutes, and obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine.

In other embodiments, when preparing liquid preparations, control the stirring speed to 40-80 rpm; after adding Anisiflupurin and triacontanol, control the stirring time to 30-60 min; after adding stabilizers and emulsifiers, control stirring The time is 30-60min; after adding the solvent, control the stirring time to 30-60min.

The liquid preparation of this example was tested for Anisiflupurin and triacontanol content, pH value, long-lasting foaming property, dilution stability, low temperature stability and heat storage stability. The results are shown in Table 30.

Table 30 Performance test results of the liquid formulation of Example 8

In order to examine the impact of different co-solvents on liquid preparations, add 35 g of co-solvents to the mixing device, accurately weigh 9.8 g of Anisiflupurin and 0.2 g of triacontanol, and stir and dissolve for 60 minutes at a speed of 40 rpm. , observe the dissolution of Anisiflupurin and triacontanol, and the results are shown in Table 31.

Table 31 Dissolution of Anisiflupurin and Triacontanol by different cosolvents

It can be seen from Table 31 that when using the same mass of n-butanol, triethanolamine, propylene carbonate, propylene glycol and N-methylpyrrolidone to dissolve the raw materials, only propylene glycol and N-methylpyrrolidone have the best dissolving effect on Anisiflupurin and triacontanol. good.

In order to examine the impact of stabilizers on liquid preparations, liquid preparations containing different stabilizers were prepared according to the preparation method of liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 8. The liquid preparations, the type of stabilizer and the dilution stability of each liquid preparation prepared are shown in Table 32.

Table 32 Types of stabilizers and dilution stability of each liquid formulation produced

It can be seen from Table 32 that when formic acid is used as the stabilizer, the dilution stability of the preparation is qualified; when aluminum formate, magnesium formate, and malic acid are used as the stabilizer, when the dilution stability is tested, precipitation appears at the bottom of the liquid preparation, which is unqualified.

When the co-solvent in Table 32 is replaced from N-methylpyrrolidone to propylene glycol or the emulsifier is replaced from Tween 80 to Span 80, the dilution stability test results are unqualified.

Finally, the influence of the dosage of each component on the liquid preparation was examined. The specific method is as follows: liquid containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine according to Example 8 The preparation method of the preparation is to prepare a liquid preparation. The difference is that the mass fraction of each component is changed. The results show that when the mass fraction of Anisiflupurin is > 1.9% and ≤ 9.8%, the mass fraction of triacontanol is > 0.1% and ≤ 0.2%. When the mass fraction of co-solvent is 30-40%, the mass fraction of stabilizer is 6-10%, the mass fraction of emulsifier is 3-7%, and the balance is solvent, the liquid preparations prepared are all clear and transparent liquids. The pH value is 2 to 5, and the test results of long-lasting foaming, dilution stability, low-temperature stability and thermal storage stability are all qualified. When the mass fraction of a certain component in the liquid preparation is not within the above numerical range, one or more of the performance indicators of the prepared liquid preparation do not meet the requirements.

Comparative example 1

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 1. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of gibberellic acid GA3 is 0.

Comparative example 2

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 2. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of gibberellic acid GA4+7 is 0.

Comparative example 3

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 3. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of 24-epibrassinolide is 0.

Comparative example 4

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 4. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of 28-homobrassinolide is 0.

Comparative example 5

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 5. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of ethylamine is 0.

Comparative example 6

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl) in Example 6. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of ethephon is 0.

Comparative example 7

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 5. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine is 0.

Comparative example 8

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl) in Example 6. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine is 0.

Comparative example 9

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Example 7. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of triacontanol is 0.

Comparative example 10

The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is different from the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl) in Example 8. The only difference between the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in this comparative example is that The mass fraction of triacontanol is 0.

Experimental example 1

In order to evaluate the effect of the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine of Examples 1 and 2 on the growth of oat coleoptiles, commercially available 4% Gibberellic acid soluble agent (produced by Zhengzhou Zhengshi Chemical Products Co., Ltd.) was used as a control agent, and water was used as a blank control. Then different preparations were diluted with water to obtain test reagents. Then test the effect of each test reagent on the growth of oat coleoptiles. The test method is as follows: Cultivate oats under dark conditions. When the oat seedlings grow to about 5cm, cut the coleoptiles from the oat seedlings, and then cut off the top of the coleoptiles ( The cut length is 6mm) to obtain the bud sheath with the top removed, and then use a quantitative scissor to uniformly cut 1 cm length from the uppermost end of the bud sheath with the top removed to obtain the bud sheath segments. Then, the cut sections of the coleoptiles were soaked in phosphate-citric acid buffer (pH=5.0) containing 2% (mass fraction) sucrose for 3 hours to wash away the endogenous auxin. Then take the washed and dried petri dishes, put 10 cut sections of bud sheaths that have been washed away of endogenous auxin into each petri dish, and then add the same test reagent (the volume is 20mL) to each petri dish, so that The cut sections of bud sheaths in the petri dish are soaked in the test reagents. At the same time, five parallel experiments are set up for each test reagent (that is, the number of petri dishes added to each test reagent is 5), and then the test reagents and Petri dishes with cut sections of coleoptiles are placed in a shaker and cultured in the dark for 24 hours or 48 hours. After the culture is completed, the growth amount of each cut section of coleoptiles in each dish is tested, and then the corresponding response of each test reagent is calculated. The average value of the growth amount of all coleoptile segments in the five petri dishes is used as the test result of the growth amount of the coleoptile segments corresponding to the corresponding test reagent. Clear water will be used as the test reagent for the corresponding buds. The test result of the growth amount of the coleoptile section is defined as M, and then the coleoptile growth promotion rate corresponding to each test reagent is calculated. The calculation formula is: coleoptile growth promotion rate (%) = (test result of the growth amount of the coleoptile section - M)/M×100. The number of each test reagent and the active ingredients in each test reagent (2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine, gibberellic acid GA3 and gibberellic acid GA4+7) The concentrations of each test reagent and the corresponding coleoptile growth promotion rates at different culture times are shown in Table 33.

Table 33: The number of each test reagent, the concentration of the active ingredient in each test reagent, and the corresponding coleoptile growth promotion rate of each test reagent at different culture times

It can be seen from Table 33 that the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine of Examples 1-2 all have the effect of promoting the growth of coleoptiles, and are better than The control agent (conventional plant conditioning agent) gibberellic acid promotes the growth of coleoptiles.

Experimental example 2

In order to evaluate the effect of the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Examples 3 and 4 on crop growth, commercially available 0.01% 24 -Epibrassinolide soluble agent (produced by Zhengzhou Zhengshi Chemical Products Co., Ltd.) and commercially available 0.01% 28-homobrassinolide soluble agent (produced by Zhengzhou Zhengshi Chemical Products Co., Ltd.) were used as control agents 1 and control agent 2, while using water as a blank control, and then diluting the different preparations with water to obtain the test reagent. Then test the effect of each test reagent on the growth of peanuts. The test method is as follows: select peanut fields with consistent growth (the peanuts in the peanut fields are all in the seedling stage), randomly divide the areas, each partition covers three ridges of peanuts, and set the settings between different partitions. A row of peanuts with a width of one ridge is used as a protective row. The area of each partition is 30m ² . Identification boards are made in different partitions, and then different test reagents are evenly sprayed on the peanut plants in different partitions with an electric sprayer in the form of spray. The number of partitions corresponding to the test reagent is 1. The quality of the liquid preparation sprayed on each peanut plant in each partition is the same, and the spraying method is also the same. Finally, the height, chlorophyll content, protein content and fat content of the peanut plants were tested 7 days after spraying. Among them, the testing methods for peanut plant height, chlorophyll content, protein content and fat content are as follows:

(1) Plant height: 10 peanut plants at different locations in each zone were randomly selected as sampling points and marked. In order to ensure the accuracy and representativeness of the data, the height of the main stem of peanuts was measured uniformly, and a steel tape measure was used to measure the height from the root to The distance between the growing points of the stem tips. The measured distance is the main stem height of the peanut plant. The average of the main stem heights of 10 randomly selected peanut plants in each zone is used as the main stem height of the peanut plants in the zone. test results.

(2) Chlorophyll content: 5 peanut plants at different locations in each zone were randomly selected as sampling points and marked. The chlorophyll content, protein content and fat content of 2 leaves in all leaves except new leaves in the upper layer of peanut plants at each sampling point were randomly measured. The average value of the chlorophyll content of the two leaves corresponding to each sampling point was used as the representative value of the chlorophyll level of the peanut plant at that sampling point. The testing instrument for the chlorophyll content was the Konica Minolta SPAD502 handheld chlorophyll meter; the average value of the two leaves corresponding to each sampling point was The average value of the protein content of the peanut plants at the sampling point was used as the representative value of the protein level of the peanut plant at the sampling point. The protein content was measured using a fully automatic Kjeldahl nitrogen analyzer; the average value of the fat content of the two leaves corresponding to each sampling point was used as the sampling point. Representative values of fat levels in peanut plants, and fat content was measured using a fat meter. Finally, calculate the average of the representative values of chlorophyll level, the average of protein level and the average of the representative value of fat level of the peanut plants at 5 different positions in each partition, and use the calculated average values as the average values of the peanut plants in the partition. The test results of chlorophyll content, protein content and fat content.

The number of each test reagent, the active ingredients in each test reagent (2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine, 24-epibrassinolide and 28-high brassinolide Lactone) concentration and the test results of peanut plant height, chlorophyll content, protein content and fat content after using each test reagent are shown in Table 34.

Table 34: The number of each test reagent, the concentration of active ingredients in each test reagent, and the test results of peanut plant height, chlorophyll content, protein content and fat content after using each test reagent

It can be seen from Table 34 that the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Examples 3-4 all have the effect of promoting the growth of peanut plants, and are consistent with Compared with the blank control group (clear water), the liquid preparations of Examples 3-4 can significantly increase the chlorophyll content, protein content and fat content in the leaves of peanut plants, can significantly promote the growth of peanuts, increase the amount of chlorophyll accumulation, and thus have It is conducive to the accumulation of photosynthetic products, promotes full fruit, increases yield and improves quality.

Experimental example 3

In order to evaluate the effect of the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Examples 5 and 6 on crop growth, 30% of the commercially available amine Fresh ester·ethephon aqueous agent (produced by Zhengzhou Zhengshi Chemical Products Co., Ltd.) was used as the control agent, and water was used as the blank control. Then different preparations were diluted with water to obtain test reagents. Then according to the method in the standard GB/T17980.145-2004 "Pesticide Field Efficacy Test Guidelines (2)", Fuji apples with the same growth rate were used as the experimental subjects. Spray the test reagents evenly on the stems and leaves of the apple plants during the fruit setting and fruit expansion stages. The area of the apple plants sprayed with each test reagent is 1 mu, and the test results of each spray on each apple plant are The quality of the reagents is the same. After each test reagent is sprayed, the presence of phytotoxicity will be investigated on the 3rd day. At the same time, the fruit setting rate, single fruit weight, quality and other data will be investigated, and the vitamin C content in the fresh fruit and the hardness of the apple fruit will be tested. During the test Randomly select 5 apple plants from all the apple plants sprayed with each test reagent as test samples, and use the average of the test results of the 5 plants as the fruit setting rate, single fruit weight, vitamin C content in fresh fruits and apples after using the corresponding test reagent. Fruit firmness test results.

The number of each test reagent, the concentration of the active ingredients in each test reagent (2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine, ampicillin and ethephon) and the use of each test reagent The test results of apple fruit setting rate, single fruit weight, vitamin C content in fresh fruit and apple fruit hardness after testing the reagent are shown in Table 35.

Table 35: The number of each test reagent, the concentration of the active ingredient in each test reagent, and the corresponding coleoptile growth promotion rate of each test reagent at different culture times

It can be seen from Table 35 that after the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine of Example 5-6 is sprayed on apple trees, the fruit setting rate of apples The weight of the single fruit has also increased, and the vitamin C content in the fruit has also increased.

Experimental example 4

In order to evaluate the effect of the liquid preparations containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in Examples 7 and 8 on crop growth, commercially available 0.1% 30 Alkanol soluble agent (produced by Zhejiang Dapeng Pharmaceutical Co., Ltd.) was used as the control agent, and water was used as the blank control. Then different preparations were diluted with water to obtain test reagents. Then the mango variety Tainong No. 1 was used as the test object. During the test, a total of 5 mango trees were applied with the same test reagent, and only one test reagent was applied to each tree. Before application, each tree was divided into east and south. , west, north, and center, mark 3 tidbits in each direction (the diameter of the young fruits on the tidbits are all 0.8-1cm, and the total amount of young fruits on the tidbits remains the same), and then immerse the marked tidbits for testing In the reagent, the test reagent is used to soak the young fruits. The drug is used twice during the test period. The interval between the two treatments is 7 days. Each tidbit is soaked in the test reagent for the same time during each use. Survey on the day before the first use of the drug. The longitudinal and transverse diameters of the fruits were measured, and then the longitudinal and transverse diameters of the fruits were measured on the 7th day after the first application of the medication. The second application of the medication was performed at the same time. The longitudinal and transverse diameters of the fruits were measured on the 7th day after the second application of the medication. Finally, the vertical and horizontal diameters of the fruits were measured on the 7th day after the first medication. The growth of the longitudinal and transverse diameter of the fruit on the 7th day compared to the vertical and horizontal diameter of the fruit on the day before the first application of the medication, and the vertical and horizontal diameter of the fruit on the 7th day after the second application of the medication compared with the vertical and horizontal diameter of the fruit on the day before the first application of the medication. diameter growth. Finally, calculate the average growth of the longitudinal and transverse diameters of all marked fruits on the 5 mango trees treated with each test agent on the 7th day after the first application and the average growth of the fruit on the 7th day after the second application. The average increase in longitudinal and transverse diameters.

The number of each test reagent, the concentration of the active ingredients (2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and triacontanol) in each test reagent, and the use of each test reagent The growth amount of the longitudinal and transverse diameter of the fruit on the 7th day after the first administration of the drug and the growth amount of the longitudinal and transverse diameter of the fruit on the 7th day after the second administration are shown in Table 36.

Table 36: The number of each test reagent, the concentration of the active ingredient in each test reagent, and the increase in the vertical and horizontal diameter of the young mango fruit by each test reagent

As can be seen from Table 36, compared with clear water, the use of the liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine of Example 7-8 has better effects on young mango fruits. After soaking, it can promote the growth of the longitudinal and transverse diameters of the fruit, and as the concentration of active ingredients increases, the promotion effect is significantly improved. In addition, when the active ingredient concentration is the same, compared with the control agent, the second administration of Example 7-8 containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine On the 7th day after the liquid preparation, the growth of the longitudinal diameter and transverse diameter of the mango fruit was significantly greater, indicating that Examples 7-8 contained 2-fluoro-N-(3-methoxyphenyl)-7H-purine. The liquid preparation of -6-amine has a better promoting effect on the growth of young mango fruits.

Claims (10)

1. A liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine, which is characterized in that it mainly consists of active ingredients, emulsifiers, solvents and co-solvents; The active ingredient consists of 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine and a compound; the compound is gibberellic acid GA3, gibberellic acid GA4+ 7. 24-Epibrassinolide, 28-homobrassinolide, ethanolide, ethephon or triacontanol; the co-solvent is dimethyl sulfoxide, N,N-dimethylformamide , N-methylpyrrolidone, lactic acid; the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine in the liquid preparation is not greater than 10%; in the liquid preparation The mass fraction of the compound shall not be greater than 30%. 2. The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as claimed in claim 1, characterized in that the compound is gibberellic acid GA3, the mass fractions of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and gibberellic acid GA3 in the liquid preparation are independently no more than 0.9%; Or the compound is gibberellic acid GA4+7, and the liquid preparation contains 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine and gibberellic acid GA4+7. The mass fraction is independently not more than 1.8%; Or the compound is 24-epibrassinolide, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.99%, the mass fraction of 24-epibrassinolide is not more than 0.01%; Or the compound is 28-homobrassinolide, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 4.995% , the mass fraction of 28-homobrassinolide is not greater than 0.005%; Or the compound is ethylamine, the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not more than 2%, and the ethylamine is The mass fraction of ester is not more than 8%; Alternatively, the compound is ethephon, the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 0.5%, and ethephon is The mass fraction is not greater than 29.5%; Or the compound is triacontanol, the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not more than 9.8%, and the three The mass fraction of decanol is not greater than 0.2%. 3. The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as claimed in claim 1 or 2, characterized in that the compound is erythroid Mycolic acid GA3, gibberellic acid GA4+7, 24-epibrassinolide, 28-homobrassinolide, triacetate or triacontanol, the liquid preparation also includes a stabilizer, and the stabilizer is Formic acid, acetic acid, lactic acid, 2,6-di-tert-butyl-p-cresol, tert-butylhydroquinone. 4. The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as claimed in claim 3, characterized in that the compound is gibberellic acid GA3, the stabilizer is formic acid and/or lactic acid; Or the compound is gibberellic acid GA4+7, and the stabilizer is formic acid and/or lactic acid; Or the compound is 24-epibrassinolide, and the stabilizer is formic acid and/or acetic acid; Or the compound is 28-homobrassinolide, and the stabilizer is formic acid and/or acetic acid; Or the compound is ethyl ester, and the stabilizer is 2,6-di-tert-butyl-p-cresol or tert-butyl hydroquinone; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The mass fraction of triacontanol in the liquid preparation is not greater than 0.1%, and the stabilizer in the liquid preparation is acetic acid; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, and the stabilizer in the liquid preparation is formic acid. 5. The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as claimed in claim 4, characterized in that the compound is gibberellic acid GA3, the mass fraction of the stabilizer in the liquid preparation is 0.5 to 2%; Or the compound is gibberellic acid GA4+7, and the mass fraction of the stabilizer in the liquid preparation is 10 to 20%; Or the compound is 24-epibrassinolide, and the mass fraction of the stabilizer in the liquid preparation is 3 to 6%; Or the compound is 28-homobrassinolide, and the mass fraction of the stabilizer in the liquid preparation is 0.3 to 0.6%; Or the compound is ethyl ethyl, and the mass fraction of the stabilizer in the liquid preparation is 1 to 3%; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The mass fraction of triacontanol in the liquid preparation is not greater than 0.1%, and the mass fraction of the stabilizer in the liquid preparation is 1 to 3%; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, and the mass fraction of the stabilizer in the liquid preparation is 6 to 10%. 6. The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as claimed in claim 1 or 2, characterized in that the compound is erythroid Mycolic acid GA3, the emulsifier in the liquid preparation is octylphenol polyoxyethylene ether and/or fatty alcohol polyoxyethylene ether; the mass fraction of the emulsifier in the liquid preparation is 1 to 4%; Or the compound is gibberellic acid GA4+7, and the emulsifier in the liquid preparation is octylphenol polyoxyethylene ether and/or fatty alcohol polyoxyethylene ether; the quality of the emulsifier in the liquid preparation The score is 2 to 4%; Or the compound is 24-epibrassinolide, and the emulsifier in the liquid preparation is Tween 80; the mass fraction of the emulsifier in the liquid preparation is 3 to 7%; Or the compound is 28-homobrassinolide, the emulsifier in the liquid preparation is polyethylene glycol 400; the mass fraction of the emulsifier in the liquid preparation is 4 to 8%; Or the compound is ethyl ethyl, and the emulsifier in the liquid preparation is polyether; the mass fraction of the emulsifier in the liquid preparation is 0.5 to 2%; Or the compound is ethephon, and the emulsifier in the liquid preparation is polyethylene glycol 400; the mass fraction of the emulsifier in the liquid preparation is 3 to 6%; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The emulsifier in the liquid preparation is fatty alcohol polyoxyethylene ether; the mass fraction of the emulsifier in the liquid preparation is 2 to 6%; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, the emulsifier in the liquid preparation is Tween 80; the mass fraction of the emulsifier in the liquid preparation is 3~ 7%. 7. The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as claimed in claim 1 or 2, characterized in that the compound is erythroid Mycolic acid GA3, the co-solvent in the liquid preparation is dimethyl sulfoxide; the mass fraction of the co-solvent in the liquid preparation is 15 to 25%; Or the compound is gibberellic acid GA4+7, and the co-solvent in the liquid preparation is N,N-dimethylformamide; the mass fraction of the co-solvent in the liquid preparation is 30 to 40%; Or the compound is 24-epibrassinolide, and the co-solvent in the liquid preparation is N-methylpyrrolidone and/or dimethyl sulfoxide; the mass fraction of the co-solvent in the liquid preparation is 10~20%; Or the compound is 28-homobrassinolide, the co-solvent in the liquid preparation is N-methylpyrrolidone and/or dimethyl sulfoxide; the mass fraction of the co-solvent in the liquid preparation is 20 ~40%; Or the compound is ethyl acetate, and the co-solvent in the liquid preparation is N-methylpyrrolidone; the mass fraction of the co-solvent in the liquid preparation is 20 to 30%; Or the compound is ethephon, the co-solvent in the liquid preparation is lactic acid; the mass fraction of the co-solvent in the liquid preparation is 30 to 40%; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The mass fraction of triacontanol in the liquid preparation is not greater than 0.1%, the co-solvent in the liquid preparation is dimethyl sulfoxide; the mass fraction of the co-solvent in the liquid preparation is 10 to 20%; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, the co-solvent in the liquid preparation is N-methylpyrrolidone; the mass fraction of the co-solvent in the liquid preparation is 30~40%. 8. The liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as claimed in claim 1 or 2, characterized in that the compound is erythroid Mycolic acid GA3, the solvent in the liquid preparation is an alcoholic solvent, and the alcoholic solvent is ethylene glycol and/or propylene glycol; Or the compound is gibberellic acid GA4+7, the solvent in the liquid preparation is an alcoholic solvent, and the alcoholic solvent is ethylene glycol and/or propylene glycol; Or the compound is 24-epibrassinolide, and the solvent in the liquid preparation is propylene glycol; Or the compound is 28-homobrassinolide, and the solvent in the liquid preparation is N,N-dimethylformamide; Or the compound is ethylcarboxylate, and the solvent in the liquid preparation is N,N-dimethylformamide; Or the compound is ethephon, and the solvent in the liquid preparation is selected from one or any combination of water, ethylene glycol, and propylene glycol; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is not greater than 1.9%, so The mass fraction of triacontanol in the liquid preparation is not greater than 0.1%, and the solvent in the liquid preparation is propylene glycol; Or the compound is triacontanol, and the mass fraction of 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine in the liquid preparation is >1.9% and ≤9.8 %, the mass fraction of triacontanol in the liquid preparation is >0.1% and ≤0.2%, and the solvent in the liquid preparation is N,N-dimethylformamide. 9. A method for preparing a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purine-6-amine as claimed in any one of claims 1 to 8, characterized by The method includes the following steps: uniformly mixing the components of the formula to obtain a liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine. 10. A liquid preparation containing 2-fluoro-N-(3-methoxyphenyl)-7H-purin-6-amine as described in any one of claims 1-8 as a plant growth regulator application.