CN116718782A - Carboxyl receptor microsphere modified by polyethylene glycol derivative and preparation method and application thereof - Google Patents
Carboxyl receptor microsphere modified by polyethylene glycol derivative and preparation method and application thereof Download PDFInfo
- Publication number
- CN116718782A CN116718782A CN202310692362.0A CN202310692362A CN116718782A CN 116718782 A CN116718782 A CN 116718782A CN 202310692362 A CN202310692362 A CN 202310692362A CN 116718782 A CN116718782 A CN 116718782A
- Authority
- CN
- China
- Prior art keywords
- carboxyl
- polyethylene glycol
- glycol derivative
- microsphere
- modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Endocrinology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicinal Preparation (AREA)
- Steroid Compounds (AREA)
Abstract
The application relates to a carboxyl receptor microsphere modified by polyethylene glycol derivatives, a preparation method and application thereof, wherein the carboxyl receptor microsphere modified by the polyethylene glycol derivatives is generated by chemical reaction between carboxyl groups of the carboxyl receptor microsphere and active groups of the polyethylene glycol derivatives; the active group of the polyethylene glycol derivative is amino, sulfhydryl, hydroxyl, carboxyl, aldehyde group, methoxy and/or maleimide. After the surface modification of carboxyl receptor microspheres in photo-excited homogeneous luminescence by the polyethylene glycol derivative, when the application is used for photo-excited homogeneous luminescence estradiol detection test, the correlation performance of a test sample is better compared with an estradiol antibody reagent which is not subjected to the surface modification of the carboxyl receptor microspheres, wherein the correlation performance of the test sample is better due to the fact that the background or false positive is greatly reduced due to the adsorption of non-specific immunoglobulin (IgG) caused by the increase of the sample quantity to be tested in 10 mu L to 50 mu L.
Description
Technical Field
The application relates to the technical field of biological detection, in particular to a carboxyl receptor microsphere modified by polyethylene glycol derivatives, and a preparation method and application thereof.
Background
Estradiol is a natural estrogen with a molecular weight of 272.3 KD. Currently, the main methods for clinically measuring estradiol include chemiluminescence immunoassay, liquid chromatography tandem mass spectrometry and the like. Light-activated chemiluminescence analysis is gradually accepted by clinical laboratories and is applied to a certain extent as a novel chemiluminescence analysis method. The technology is different from electrochemiluminescence, direct chemiluminescence and enzymatic chemiluminescence, and adopts two marks of a luminescent substance and a photosensitive substance to be respectively distributed in the luminescent microsphere and the photosensitive microsphere. The two microspheres are combined by virtue of antigen-antibody, so that the transfer of high-energy active oxygen is realized, and the photoexcitation chemiluminescence process is induced, thereby realizing the 'no-clean' homogeneous immunoassay, and having high precision and high detection speed.
In clinical detection of estradiol in blood, the sample loading amount of a blood sample to be detected is large due to the requirement of high sensitivity, but any blood sample is a mixture with complex components, and various interference substances influencing immune response exist. Because of the characteristic of 'no-clean' of the photo-excitation chemiluminescence technology, the photo-excitation chemiluminescence technology is particularly easy to be influenced by interferents, if a large number of samples are added, the influence caused by the interferents is larger, and the interferents can generate nonspecific adsorption on the microspheres, so that abnormal change of signal values is caused, and false negative or false positive is caused. Therefore, the nonspecific adsorption of the interfering substances caused by high sample loading is a problem to be solved in the clinical detection of the estradiol at present.
Disclosure of Invention
The application provides a carboxyl receptor microsphere modified by polyethylene glycol derivatives, and a preparation method and application thereof, which are used for solving the problem of nonspecific interference in a sample for detecting estradiol in blood by using a photo-activated chemiluminescence technology.
The application provides a carboxyl receptor microsphere modified by polyethylene glycol derivatives, which is generated by chemical reaction of carboxyl groups of the carboxyl receptor microsphere and active groups of the polyethylene glycol derivatives;
the active group of the polyethylene glycol derivative is amino, sulfhydryl, hydroxyl, carboxyl, aldehyde group, methoxy and/or maleimide.
Further, the active groups of the polyethylene glycol derivative are amino and carboxyl.
Further, the molecular weight of the polyethylene glycol derivative is 400-20000, and the mass ratio of the carboxyl acceptor microsphere to the polyethylene glycol derivative is 1 (0.1-10).
Further, the molecular weight of the polyethylene glycol derivative is 2000, and the mass ratio of the carboxyl acceptor microsphere to the polyethylene glycol derivative is 1:1.
The application also provides a preparation method of the carboxyl receptor microsphere modified by the polyethylene glycol derivative, which comprises the following steps:
adding a crosslinking activator into the carboxyl receptor microsphere, uniformly mixing, then carrying out shaking table incubation, centrifuging, discarding supernatant, then carrying out heavy suspension by using a buffer solution, then adding a polyethylene glycol derivative, the crosslinking activator and a surfactant, then carrying out constant temperature reaction, centrifuging after the constant temperature reaction, discarding supernatant, then adding the buffer solution, then carrying out ultrasonic treatment, centrifuging, discarding supernatant, and washing to obtain the carboxyl receptor microsphere.
Further, the technological parameters of the shaking table incubation include: the temperature is 35-40 ℃ and the time is 20-40 min;
the technological parameters of the constant temperature reaction comprise: the temperature is 35-40 ℃, the rotating speed is 800-1200 rpm, and the reaction time is 1.8-2.2 h.
Further, the crosslinking activator is at least one of EDC, NHS, CMC, DTSSP, DMP, SMPT and SMCC with the concentration of 8-12 mg/mL;
the buffer solution is at least one of 2-morpholinoethanesulfonic acid buffer (MES), citrate buffer, phosphate buffer, tris-HCL buffer, hydroxyethylpiperazine ethylsulfuric acid buffer (HEPES), carbonate-bicarbonate buffer and N- [ Tris (hydroxymethyl) methyl ] -3-aminopropanesulfonic acid buffer (TAPS);
the surfactant is at least one of Tween-20, tween-40, tween-60, tween-80, triton X-100, triton X-405, sodium deoxycholate, glycerol stearate, polyvinylpyrrolidone (PVP) and laurylpyrrolidone with concentration of 8-12 wt%.
The application also provides application of the carboxyl acceptor microsphere modified by the polyethylene glycol derivative in detecting the light-excited homogeneous luminescent estradiol.
The application also provides a carboxyl receptor microsphere modified by polyethylene glycol derivatives coated with the estradiol antibody, which is obtained by coating the surface of the estradiol antigen by the carboxyl receptor microsphere modified by the polyethylene glycol derivatives.
Compared with the prior art, the technical scheme provided by the embodiment of the application has the following advantages:
the adsorption of the microsphere and the protein mainly depends on the hydrophobic acting force of a hydrophobic section of the protein and the surface of the microsphere, and the polyethylene glycol has good hydrophilicity. When the carboxyl receptor microsphere modified by the polyethylene glycol derivative is used for photoexcitation homogeneous luminescent estradiol detection test, the high negative background or false positive caused by non-specific immunoglobulin (IgG) adsorption along with the increase of the sample to be tested in 10 mu L to 50 mu L is greatly reduced, and the correlation performance of a test sample is better compared with an estradiol antibody reagent which is not subjected to surface modification of the carboxyl receptor microsphere.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the application and together with the description, serve to explain the principles of the application.
In order to more clearly illustrate the embodiments of the application or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, and it will be obvious to a person skilled in the art that other drawings can be obtained from these drawings without inventive effort.
FIG. 1 is a schematic diagram showing the modification process of the polyethylene glycol derivative modified carboxyl acceptor microsphere according to the embodiment 1 of the present application;
fig. 2 is a schematic diagram of a detection reaction of a carboxyl acceptor microsphere modified by polyethylene glycol derivative provided in example 1 of the present application.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments, based on the embodiments of the application, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the application. Thus, the following detailed description of the embodiments of the application, as presented in the figures, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application.
In the description of the present application, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature.
Unless otherwise specifically indicated, the various raw materials, reagents, instruments, equipment and the like used in the present application are commercially available or may be prepared by existing methods.
Example 1
A carboxyl receptor microsphere modified by polyethylene glycol derivative, the preparation method comprises:
placing 1mL of 200nm carboxyl acceptor microsphere (concentration is 10mg/mL, biological Cat: 67700005) in a centrifuge, centrifuging at 14000rpm for 15min, discarding supernatant, adding 1mL of 0.05M, pH MES buffer solution for 2 times, adding 10 mu L of 10mg/mL EDC and NHS into the carboxyl acceptor microsphere, mixing, incubating at 37deg.C for 30min, centrifuging, discarding supernatant, re-suspending with 0.05M, pH HEPES buffer solution, adding polyethylene glycol derivative (NH) with molecular weight of 2000Da according to the mass ratio of carboxyl acceptor microsphere to polyethylene glycol derivative of 1:1 4 -(PEG) n -COOH), 10 mu L of 10mg/mL EDC is added and mixed uniformly, 10 mu L of 10wt% Tween-20 is added and mixed uniformly, then the mixture is reacted for 2 hours in a constant temperature mixer at 37 ℃ and 1300rpm, the mixture is placed in a centrifuge and centrifuged at 14000rpm for 15 minutes, the supernatant is discarded, 1mL of 0.05M, pH MES buffer with 5 is added, ultrasonic centrifugation is carried out, and the mixture is cleared with 0.05M, pH MES buffer with 5Washing for 2 times to obtain the carboxyl acceptor microsphere modified by polyethylene glycol derivative.
Example 2
The mass ratio of the carboxyl acceptor microsphere to the polyethylene glycol derivative in example 1 was changed to 1:0.1, and the rest was the same as in example 1.
Example 3
The mass ratio of the carboxyl acceptor microsphere to the polyethylene glycol derivative in example 1 was changed to 1:0.5, and the rest was the same as in example 1.
Example 4
The mass ratio of the carboxyl acceptor microsphere to the polyethylene glycol derivative in example 1 was changed to 1:5, and the rest was the same as in example 1.
Example 5
The mass ratio of the carboxyl acceptor microsphere to the polyethylene glycol derivative in example 1 was changed to 1:10, and the rest was the same as in example 1.
Example 6
The molecular weight of the polyethylene glycol derivative in example 1 was changed to 5000Da, and the remainder was the same as in example 1.
Example 7
The molecular weight of the polyethylene glycol derivative in example 1 was changed to 10000Da, and the remainder was the same as in example 1.
The carboxyl receptor microspheres modified with the polyethylene glycol derivatives of examples 1 to 7 and the unmodified carboxyl receptor microspheres, respectively, were coated with an estradiol antibody comprising:
the mass ratio of the polyethylene glycol derivative modified carboxyl acceptor microsphere to the unmodified carboxyl acceptor microsphere in examples 1 to 7 is 1mg: adding 25 mu g of estradiol antibody respectively, mixing uniformly, adding 5 mu L of 10mg/mL of EDC, adding 10 mu L of 10wt% Tween-20, mixing uniformly, reacting for 2 hours at 37 ℃ and 1000rpm in a constant temperature mixing instrument, performing ultrasonic centrifugation, washing for 2 times with MES buffer solution with the concentration of 0.05 and M, pH being 5, adding 50 mu L of 10% bovine serum albumin and ethanolamine respectively, sealing for 30 minutes at 1000rpm in a constant temperature mixing instrument at 37 ℃, centrifuging the reaction solution in a centrifuge for 15 minutes at 14000rpm after sealing, removing supernatant, adding Tris buffer solution with the concentration of 0.05 and M, pH being 7.4 for resuspension, repeating centrifugation, removing supernatant and resuspension operation for 2 times, and finally adding Tris buffer solution with the concentration of 0.05 and M, pH being 7.4 for preservation, thereby obtaining the carboxyl acceptor microsphere coated with the estradiol antibody.
The application effect of the obtained carboxyl acceptor microsphere coated with the estradiol antibody on a photo-excited homogeneous luminescent estradiol kit is tested by using a LiCA luminescent reader, and the results are shown in tables 1-3.
Table 1: effect of carboxyl acceptor microsphere and polyethylene glycol derivative with different mass ratios
Table 2: effects of polyethylene glycol derivatives of different molecular weights
Table 3: effect of 50 μl of estradiol sample
As can be seen from Table 3, when the amount of the estradiol sample to be added is 50. Mu.L, the abnormal change of the signal value caused by nonspecific adsorption of the interferents is greatly reduced by using the carboxyl receptor microsphere modified by the polyethylene glycol derivative, and the correlation performance of the test sample is better compared with the estradiol antibody receptor microsphere reagent coated by the surface modification of the carboxyl receptor microsphere.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, and various modifications and variations may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (10)
1. A carboxyl acceptor microsphere modified by polyethylene glycol derivative, which is characterized in that: the carboxyl receptor microsphere modified by the polyethylene glycol derivative is generated by chemical reaction of carboxyl of the carboxyl receptor microsphere and an active group of the polyethylene glycol derivative;
the active group of the polyethylene glycol derivative is amino, sulfhydryl, hydroxyl, carboxyl, aldehyde group, methoxy and/or maleimide.
2. The polyethylene glycol derivative modified carboxyl acceptor microsphere according to claim 1, wherein: the active groups of the polyethylene glycol derivative are amino and carboxyl.
3. The polyethylene glycol derivative modified carboxyl acceptor microsphere according to claim 1, wherein: the molecular weight of the polyethylene glycol derivative is 400-20000, and the mass ratio of the carboxyl acceptor microsphere to the polyethylene glycol derivative is 1 (0.1-10).
4. A polyethylene glycol derivative modified carboxyl acceptor microsphere according to claim 3 wherein the polyethylene glycol derivative has a molecular weight of 2000 and the mass ratio of carboxyl acceptor microsphere to polyethylene glycol derivative is 1:1.
5. The method for preparing a carboxyl acceptor microsphere modified by polyethylene glycol derivative according to any one of claims 1 to 4, wherein the method comprises:
adding a crosslinking activator into the carboxyl receptor microsphere, uniformly mixing, then carrying out shaking table incubation, centrifuging, discarding supernatant, then carrying out heavy suspension by using a buffer solution, then adding a polyethylene glycol derivative, the crosslinking activator and a surfactant, then carrying out constant temperature reaction, centrifuging after the constant temperature reaction, discarding supernatant, then adding the buffer solution, then carrying out ultrasonic treatment, centrifuging, discarding supernatant, and washing to obtain the carboxyl receptor microsphere.
6. The method according to claim 5, wherein the technological parameters of the shaker incubation include: the temperature is 35-40 ℃ and the time is 20-40 min;
the technological parameters of the constant temperature reaction comprise: the temperature is 35-40 ℃, the rotating speed is 800-1200 rpm, and the reaction time is 1.8-2.2 h.
7. The method of manufacturing according to claim 5, wherein: the crosslinking active agent is at least one of EDC, NHS, CMC, DTSSP, DMP, SMPT and SMCC with the concentration of 8-12 mg/mL;
the buffer solution is at least one of 2-morpholinoethanesulfonic acid buffer (MES), citrate buffer, phosphate buffer, tris-HCL buffer, hydroxyethylpiperazine ethylsulfuric acid buffer (HEPES), carbonate-bicarbonate buffer and N- [ Tris (hydroxymethyl) methyl ] -3-aminopropanesulfonic acid buffer (TAPS);
the surfactant is at least one of Tween-20, tween-40, tween-60, tween-80, triton X-100, triton X-405, sodium deoxycholate, glycerol stearate, polyvinylpyrrolidone (PVP) and laurylpyrrolidone with concentration of 8-12 wt%.
8. The method of manufacturing according to claim 7, wherein: the crosslinking active agent is EDC and NHS;
the buffer solution is hydroxyethyl piperazine ethyl sulfate buffer (HEPES) and 2-morpholinoethanesulfonic acid buffer (MES);
the surfactant is Tween-20.
9. Use of the polyethylene glycol derivative modified carboxyl acceptor microsphere according to any one of claims 1-4 in light-excited homogeneous luminescent estradiol detection.
10. A carboxyl receptor microsphere modified by polyethylene glycol derivatives coated with estradiol antibodies, characterized in that: the carboxyl acceptor microsphere modified by the polyethylene glycol derivative according to any one of claims 1 to 4, wherein the estradiol antigen is coated on the surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310692362.0A CN116718782A (en) | 2023-06-12 | 2023-06-12 | Carboxyl receptor microsphere modified by polyethylene glycol derivative and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310692362.0A CN116718782A (en) | 2023-06-12 | 2023-06-12 | Carboxyl receptor microsphere modified by polyethylene glycol derivative and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116718782A true CN116718782A (en) | 2023-09-08 |
Family
ID=87872907
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310692362.0A Pending CN116718782A (en) | 2023-06-12 | 2023-06-12 | Carboxyl receptor microsphere modified by polyethylene glycol derivative and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116718782A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117269513A (en) * | 2023-09-22 | 2023-12-22 | 厦门宝太和瑞生物技术有限公司 | Progesterone detection kit, preparation and application methods |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441514A (en) * | 2019-06-18 | 2019-11-12 | 北京利德曼生化股份有限公司 | A kind of preparation method, product and its application of latex microsphere and antibody complex |
| CN111077304A (en) * | 2019-12-31 | 2020-04-28 | 深圳市瀚德标检生物工程有限公司 | Coupling method of carboxyl latex microspheres and antibody |
| CN112175225A (en) * | 2020-09-25 | 2021-01-05 | 上海宝敏生物科技有限公司 | Polyethylene glycol modified solid phase surface and preparation method and application thereof |
| CN113125696A (en) * | 2019-12-31 | 2021-07-16 | 科美诊断技术股份有限公司 | Estradiol homogeneous phase chemiluminescence detection kit and application thereof |
| WO2022104534A1 (en) * | 2020-11-17 | 2022-05-27 | 深圳上泰生物工程有限公司 | Kit for chemiluminescence immunoassay, and preparation method therefor and application thereof |
-
2023
- 2023-06-12 CN CN202310692362.0A patent/CN116718782A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110441514A (en) * | 2019-06-18 | 2019-11-12 | 北京利德曼生化股份有限公司 | A kind of preparation method, product and its application of latex microsphere and antibody complex |
| CN111077304A (en) * | 2019-12-31 | 2020-04-28 | 深圳市瀚德标检生物工程有限公司 | Coupling method of carboxyl latex microspheres and antibody |
| CN113125696A (en) * | 2019-12-31 | 2021-07-16 | 科美诊断技术股份有限公司 | Estradiol homogeneous phase chemiluminescence detection kit and application thereof |
| CN112175225A (en) * | 2020-09-25 | 2021-01-05 | 上海宝敏生物科技有限公司 | Polyethylene glycol modified solid phase surface and preparation method and application thereof |
| WO2022104534A1 (en) * | 2020-11-17 | 2022-05-27 | 深圳上泰生物工程有限公司 | Kit for chemiluminescence immunoassay, and preparation method therefor and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117269513A (en) * | 2023-09-22 | 2023-12-22 | 厦门宝太和瑞生物技术有限公司 | Progesterone detection kit, preparation and application methods |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102426231B (en) | Immunochromatography reagent strip and sealing agent composition used for same | |
| CN103097895B (en) | Reagent composition for nucleic acid chromatography or immunochromatography, method for measurement by nucleic acid chromatography or immunochromatography, and kit for measurement by nucleic acid chromatography or immunochromatography | |
| US5710006A (en) | Reagents for specific binding assays | |
| FI102921B (en) | Method for Preparation of Biologically Active Reagents in Socks from iimide-Containing Polymers, Analytical Element and Methods for Using Them | |
| US4060597A (en) | Serological reagent and preparation thereof | |
| US4320194A (en) | Process for the production of carrier particles from yeast cells and for producing diagnostic agents therefrom and test kits containing such agents | |
| CN108508001B (en) | Chemiluminescence detection kit | |
| CN110907639A (en) | Serum amyloid protein A detection kit and preparation method thereof | |
| CN101310186A (en) | Method for determining antigen and kit therefor | |
| CN110824160A (en) | Anti-cyclic citrullinated peptide antibody detection kit and preparation method thereof | |
| CN116718782A (en) | Carboxyl receptor microsphere modified by polyethylene glycol derivative and preparation method and application thereof | |
| CN112763703B (en) | Immunomagnetic bead and preparation method and application thereof | |
| AU619019B2 (en) | An article for performing immunological assays utilizing organic dyes and methods for producing and utilizing same | |
| CN113125741A (en) | Procalcitonin detection reagent, kit, system and detection method | |
| JPH0613582B2 (en) | Polyfunctional hydrophilic spherical fine particles, method for producing the same and method for using the same | |
| CN101446586A (en) | Immunological assay reagents and assay method | |
| CN1264016C (en) | Insoluble carrier particle nephelometric immunoassay reagent | |
| JPH03502248A (en) | Aqueous washing solution for assay, diagnostic test kit and method for measuring herpes simplex virus | |
| CN116430039A (en) | Acceptor reagent for detecting novel coronavirus and application thereof | |
| WO1999060401A1 (en) | Immunoassay reagents and immunoassay method | |
| JPS6271861A (en) | Immunological-reaction component measurement method and reagent for executing said method | |
| CN107247144B (en) | Method for pretreating hepatitis C antigen and detection kit | |
| CN110118874A (en) | A kind of collaurum re-suspension liquid, the preparation method and application of colloid gold reagent, colloid gold reagent | |
| CN109188001A (en) | A kind of detection reagent of 25-hydroxy-vitamin D and preparation method thereof and kit | |
| JP3228791B2 (en) | Measuring method of antigen or antibody in sample |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |