CN116670263A - Air bubble removal device for removing air bubbles from medium and medium circulation type cell culture system including same - Google Patents

Abstract

The present invention provides an air bubble removal device for removing air bubbles from a culture medium. An air bubble removing device for removing air bubbles from a culture medium according to an exemplary embodiment of the present invention includes: a casing including an inner space, an inflow port, and an outflow port, the inner space is used to store a predetermined amount of the above-mentioned culture medium, and the inflow port allows the above-mentioned The culture medium flows into the inner space from the outside, and the outflow port discharges the culture medium in the state of removing air bubbles to the outside in the inner space; The state maintains the above-mentioned shell, and the above-mentioned internal space includes: a first space, filled with a predetermined amount of the above-mentioned culture medium; a second space, located on the upper part of the above-mentioned first space, not filled with the above-mentioned culture medium, and the above-mentioned first space and the second space are based on the The water level of the above-mentioned culture medium that flows into the above-mentioned internal space through the above-mentioned inflow port from the outside is divided.

Description

Air bubble removal device for removing air bubbles from culture medium and medium circulation system including same Ring cell culture system

technical field

The present invention relates to an air bubble removing device for removing air bubbles from a medium, and a medium circulating cell culture system including the same.

Background technique

Cell culture refers to supplying nutrients in a container to a tissue piece excised from a multicellular organism to cultivate and proliferate the tissue piece.

Animal cells derived from human or animal tissues can be cultured in a state of being suspended in a medium or attached to a support. Cells mainly derived from blood cells (including hematopoietic stem cells) are suspension cells, and cells derived from tissues such as skin, liver, or lung, embryonic stem cells, or mesenchymal stem cells are adherent cells.

Suspension cells can proliferate in a state in which cells are suspended in a medium alone, but adherent cells can proliferate only in a state in which they are attached to the surface of a support.

Therefore, in order to smoothly culture adherent cells, it is important that the cells to be cultured stably adhere to the surface of the support.

The inoculation step of attaching such adherent cells to the surface of the support is performed by injecting a medium containing the cells to be cultured into the container in which the support is placed. Right now,

When the medium comes into contact with the surface of the support, cultured cells included in the medium adhere to the surface of the support.

However, if air bubbles are included in the medium supplied from the outside during the inoculation step, the air bubbles included in the medium prevent cells from stably attaching to the surface of the support.

Therefore, when the cells are cultured in a state where the cells are not stably attached to the surface of the support, there is a problem that the cells cannot be stably cultured.

At the same time, when the culture medium required for culturing cells is supplied to the cell culture device in a medium circulation type cell culture system in which the culture medium required for culturing cells is circulated from the medium supply device to the cell culture device, the culture medium including air bubbles is supplied to the cell culture device. Cells on the support cannot smoothly supply nutrients included in the medium due to air bubbles.

Thus, there is a problem that cells that cannot be smoothly supplied with nutrients from the culture medium cannot be smoothly cultured.

Therefore, it is necessary to remove air bubbles contained in the culture medium not only in the seeding process of attaching cells to the surface of the support but also in the process of culturing the cells.

Contents of the invention

technical problem

The present invention was conceived in consideration of the above-mentioned problems, and an object of the present invention is to provide a bubble removal device for removing bubbles from a culture medium capable of supplying a culture medium in a state where bubbles are removed to a support body side of a cell culture device, and a device including the same. Medium circulation cell culture system.

solution to the problem

In order to solve the above-mentioned problems, the present invention provides an air bubble removal device for removing air bubbles from a culture medium, which includes: a casing including an inner space, an inflow port, and an outflow port, the inner space is used to store a predetermined amount of the above-mentioned culture medium, and the above-mentioned The inflow port allows the culture medium to flow into the internal space from the outside, and the outflow port discharges the culture medium in a state where air bubbles have been removed in the internal space to the outside; The shell is maintained at a predetermined height, and the internal space includes: a first space filled with a predetermined amount of the culture medium; a second space located above the first space and not filled with the culture medium; the first space and the second space are The two spaces are divided by the water level of the culture medium flowing from the outside into the inner space through the inflow port.

In addition, the above-mentioned inlet can be arranged on the above-mentioned housing so as to be located at a position relatively higher than the above-mentioned outlet, and the above-mentioned outlet can be arranged on the above-mentioned housing so as to be located at a position communicating with the above-mentioned first space, and the above-mentioned inlet can be provided on the above-mentioned housing so as to be located at a position communicating with the above-mentioned second space.

In addition, the second space may be an air bubble separation space where the culture medium flowing into the inner space through the inflow port falls into the first space and separates air bubbles.

In addition, the outflow port may be disposed on the casing so as to be located on the bottom surface of the internal space.

In addition, the air bubble removal device for removing air bubbles from the culture medium may further include an air outlet, and the air outlet is provided in the housing so as to communicate with the second space.

In this case, the above-mentioned air bubble removal device for removing air bubbles from the culture medium may further include a pressure adjustment unit, and the pressure adjustment unit is combined with the above-mentioned exhaust port so as to be able to adjust the internal pressure of the above-mentioned internal space to adjust the pressure stored in the medium. The amount of the above-mentioned culture medium in the above-mentioned inner space.

In addition, the housing may include: a box-shaped housing body having the inner space; and a cover member detachably coupled to the housing body so as to cover an open upper portion of the inner space.

In addition, the air bubble removal device for removing air bubbles from a culture medium may further include a sensing unit including a sensor for detecting a state of a predetermined amount of the culture medium stored in the inner space.

In addition, the sensor may include at least one of a pH sensor for detecting the pH of the culture medium and a temperature sensor for measuring the temperature of the culture medium.

In addition, the above-mentioned sensor may be configured such that a part of the length of one end part is immersed in the culture medium filled in the above-mentioned first space.

In addition, the sensing unit can be detachably combined with one side of the housing.

Also, the aforementioned medium may be an inoculation medium including cells to be cultured or a culture medium including nutrient components.

In another aspect, the present invention provides a medium circulation type cell culture system, which includes: a cell culture device provided with at least one support to which cells to be cultured are attached; a medium supply device internally storing a prescribed amount of A culture medium for the above-mentioned cells; a cell supply source storing a predetermined amount of a medium for inoculation including cells to be attached to the above-mentioned support; base or the air bubbles in the above-mentioned inoculation medium flowing from the above-mentioned cell supply source, and then supply to one side of the above-mentioned cell culture device; and a pump for circulating the above-mentioned culture medium, and the above-mentioned air bubble removal device is used Air bubble removal device for removing air bubbles from culture medium.

The effect of the invention

According to the present invention, the medium for inoculation or the medium for culture can be supplied to the side of the support included in the cell culture device in a state where air bubbles are removed. Thus, when the air bubble removal device for removing air bubbles from a medium of the present invention is used, cells can be stably attached to the surface of the support, and the cells to be cultured can be smoothly supplied with nutrients from the medium, and stable cell culture.

Description of drawings

FIG. 1 is a schematic diagram showing an air bubble removal device for removing air bubbles from a medium according to an embodiment of the present invention.

FIG. 2 is a diagram showing a state in which main structures in FIG. 1 are separated.

FIG. 3 is a cutaway view of the housing of FIG. 1 .

Fig. 4 is a diagram showing a state in which the inner space in Fig. 3 is filled with a culture medium.

Fig. 5 is a schematic diagram showing a medium circulating cell culture system to which an air bubble removing device for removing air bubbles from a medium according to an embodiment of the present invention is applied.

Detailed ways

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art of the present invention can easily implement them. The invention can be implemented in many different ways and is not limited to the embodiments described here. In the drawings, in order to clearly describe the present invention, parts irrelevant to the description are omitted, and the same reference numerals are attached to the same or similar structural elements throughout the specification.

As shown in FIG. 1 , an air bubble removal device 100 (hereinafter referred to as "bubble removal device") for removing air bubbles from a culture medium according to an embodiment of the present invention may include a housing 110 and a support part 120, and the medium flowing into the inside Can be discharged to the outside with air bubbles removed.

Wherein, the above-mentioned medium may be a medium for inoculation containing cells to be cultured, or a medium for culturing containing nutrients required for culturing cells.

That is, the above-mentioned inoculation medium may be a medium that includes the cells to be cultured, and the above-mentioned culture medium may be a medium that includes nutrients required for culturing the cells but does not include the cells to be cultured.

As an example, as shown in FIG. 5 , an air bubble removal device 100 according to an embodiment of the present invention can be applied to a medium circulation type cell culture system 1000 for circulating a medium.

In this case, the inoculation medium containing the cells to be cultured can be supplied from the cell supply source 200 to the cell culture apparatus 400 side, and the cells included in the inoculation medium can be attached to the support provided in the above-mentioned cell culture apparatus 400. body (not shown) surface.

In addition, the culture medium including the nutrient components necessary for culturing the cells can be sequentially circulated through the cell culture device 400 , the air bubble removal device 100 and the medium supply device 300 by driving the pump 500 .

That is, the air bubble removal device 100 can remove air bubbles from the culture medium flowing in from the medium supply device 300 or the inoculation medium flow in from the cell supply source 200, and then supply the air bubble removal device 400 to the side of the cell culture device 400. culture medium or inoculation medium.

Thereby, the cells included in the above-mentioned inoculation medium can stably attach to the surface of the support (not shown) provided in the above-mentioned cell culture device 400, and the cells attached to the above-mentioned support can pass through the above-mentioned culture medium. Nutrients to supply bring up smoothly.

For this reason, the above-mentioned casing 110 may include an internal space 113 of a specified volume, so that a specified amount of culture medium supplied from the outside can be stored, and the above-mentioned casing 110 may include: an inflow port 114 for allowing the above-mentioned medium to flow from the outside to the above-mentioned inside. The space 113 flows in; the outflow port 115 discharges the culture medium stored in the above-mentioned internal space 113 to the outside.

In this case, the inflow port 114 can be connected to the medium supply device 300 or the cell supply source 200 through a connection tube 810 , and the outflow port 115 can be connected to the medium supply device 300 through a connection tube 820 .

Thus, a predetermined amount of the culture medium flowing in from the medium supply device 300 through the inlet 114 and the connecting pipe 810 or the inoculation medium flowing in from the cell supply source 200 can be stored in the internal space 113 in a predetermined amount. The culture medium in the internal space 113 can be discharged to the outside through the outflow port 115 .

As an example, the medium discharged through the outflow port 115 can move along the connection tube 820 to the cell culture device 400 .

As a specific example, the above-mentioned casing 110 may include: a box-shaped casing body 111 having the above-mentioned internal space 113; The open upper portion of the above-mentioned internal space 113 .

In this case, the inlet 114 may be provided on the housing body 111 to communicate with the internal space 113 , and the outlet 115 may be provided on the housing body 111 to communicate with the internal space 113 .

Moreover, the above-mentioned cover part 112 can be detachably combined with the above-mentioned shell body 111 through at least one fastening part 118 , 119 .

As an example, the above-mentioned fastening parts 118, 119 may include at least one of the first fastening part 118 and the second fastening part 119, the above-mentioned first fastening part 118 may be a known bolt part, and the above-mentioned second fastening part 119 may be a known hinged clip.

As a non-limiting example, the trade name for the aforementioned hinged clips may be Fastening Members.

Thus, the cover member 112 can be combined with the housing body 111 through at least one of the first fastening member 118 and the second fastening member 119 to seal the open upper portion of the internal space 113 .

On the other hand, in the air bubble removing device 100 according to an embodiment of the present invention, the culture medium flowing into the inner space 113 may be discharged to the outside through the outlet 115 after air bubbles are removed.

For this reason, as shown in Figure 4, above-mentioned internal space 113 can be divided into the first space 113a that fills above-mentioned culture medium and the second space 113b that does not fill above-mentioned culture medium, and above-mentioned first space 113a and second space 113b can pass to The water level of the culture medium flowing into the internal space 113 is divided, and the second space 113b may be formed to be located on the upper side of the first space 113a.

In addition, the inlet port 114 may be provided in the casing 110 so as to be located relatively higher than the outlet port 115 .

That is, the outlet 115 may be provided in the housing 110 so as to communicate with the first space 113a formed on the lower side of the interior space 113, and the inlet 114 may be provided in the housing 110 so as to communicate with the first space 113a formed on the lower side of the inner space 113. The above-mentioned second space 113b on the upper side of the internal space 113 communicates with each other.

As an example, the outlet 115 may be disposed on the casing 110 so as to be located on the bottom surface of the inner space 113 and communicate with the first space 113a.

Thus, the culture medium that flows into the internal space 113 from the outside through the inflow port 114 can move to the first space 113a through the second space 113b, and the culture medium stored in the first space 113a can pass through the outflow port 115. Exhaust to the outside.

In this case, the second space 113b may function as a bubble separation space for separating bubbles from the culture medium flowing into the internal space 113 through the inflow port 114 .

That is, the culture medium that flows into the internal space 113 from the outside through the inflow port 114 can fall along the second space 113b and separate air bubbles, and the culture medium with bubbles separated in the second space 113b can flow into the first space 113a. move.

Thus, the culture medium flowing into the inner space 113 can move to the first space 113a after removing air bubbles in the second space 113b, and the culture medium filled in the first space 113a can be removed through the outflow port 115. It is discharged to the outside in the state of air bubbles.

Therefore, when the outflow port 115 is connected to the inflow port of the cell culture device 400 , the culture medium supplied to the cell culture device 400 may be in a state in which air bubbles are completely removed.

Thus, when the air bubble removal device 100 according to an embodiment of the present invention is used, the culture medium in a state where air bubbles have been removed can be supplied to the cell culture device 400 side, and thus the cells included in the inoculation medium can stably adhere to the support. surface.

Furthermore, when the culture medium in which air bubbles have been removed is supplied to the cell culture apparatus 400 side, the cells attached to the surface of the support can be smoothly supplied with nutrients from the medium while receiving minimal pressure.

At this time, the air bubble removal device 100 according to an embodiment of the present invention can adjust the internal pressure of the internal space 113 to adjust the amount of culture medium stored in the internal space 113 .

To this end, the air bubble removal device 100 may include an exhaust port 116 disposed on the housing 110 so as to communicate with the internal space 113 , and the exhaust port 116 may be connected to a pressure regulating unit 130 .

As an example, the exhaust port 116 may be provided in the cover member 112, and the exhaust port 116 may communicate with the second space 113b.

In this case, the pressure adjusting unit 130 may include an exhaust filter 131, which may also function as an exhaust unit for exhausting air bubbles separated from the medium and filled in the second space 113b to the outside.

Thus, the air bubble removal device 100 according to an embodiment of the present invention can adjust the water level of the culture medium flowing into the inner space 113 through the opening and closing of the pressure regulating unit 130, and the inner space 113 can be divided into the above-mentioned inner space by the water level of the culture medium. The first space 113a and the second space 113b.

In addition, the water level of the culture medium flowing into the internal space 113 can be changed or maintained by opening and closing the pressure regulating unit 130 .

Accordingly, the water level of the culture medium flowing into the internal space 113 can be adjusted by the pressure regulator 130 so as to be maintained at a position relatively lower than that of the inflow port 114 .

Thus, the culture medium flowing into the internal space 113 through the inlet 114 can always move to the first space 113a through the second space 113b, and the culture medium moving to the first space 113a in the inlet 114 can be the above-mentioned The culture medium in the state where air bubbles are separated in the second space 113b.

Therefore, as described above, the culture medium discharged to the outside through the outlet 115 can always be in a state where air bubbles are removed.

The support portion 120 can support the housing 110 in a state separated by a predetermined height from the bottom surface.

As an example, the supporting part 120 may include a bottom plate 122 and a side plate 124 extending from the bottom plate 122 to a predetermined height, and one side of the housing 110 may be combined with the side plate 124 .

In this case, the outflow port 115 may be arranged so that the housing 110 is positioned on the lower side of the housing 110 in a state where the housing 110 is coupled to the support portion 120 .

That is, the housing 110 may be combined with the supporting portion 120 so that the inlet port 114 may be positioned relatively higher than the outlet port 115. The state separated from the bottom plate 122 by a predetermined height is maintained.

Thus, when a predetermined amount of culture medium flows into the internal space 113 in the state where the casing 110 and the support portion 120 are coupled, the outflow port 115 can communicate with the first space 113a, and the inflow port can communicate with the first space 113a. 114 may communicate with the above-mentioned second space 113b.

However, the shape of the support portion 120 is not limited thereto, and may be appropriately changed according to design conditions as long as it is a shape that can separate the housing 110 from the bottom surface by a predetermined height.

On the other hand, the air bubble removal device 100 according to an embodiment of the present invention may further include a sensing unit 140 including sensors 143 and 144 for detecting the state of the culture medium stored in the inner space 113 .

That is, the sensors 143 and 144 may include at least one of a pH sensor 143 for detecting the pH of the culture medium and a temperature sensor 144 for measuring the temperature of the culture medium.

In this case, as shown in FIG. 4 , the sensors 143 , 144 may be provided in the casing 110 so that at least a part thereof contacts the culture medium stored in the inner space 113 .

That is, the above-mentioned sensors 143 and 144 may be arranged such that part of the length including one end is immersed in the culture medium filled in the above-mentioned first space 113a.

Thus, the air bubble removal device 100 according to an embodiment of the present invention can monitor the state of the culture medium in the previous state supplied to the cell culture device 400 through the sensors 143 and 144 , and can supply the culture medium through the outlet 115 to the cell culture device 400 The supply medium is maintained at its optimum condition.

At this time, the sensing part 140 can be detachably combined with one side of the casing 110 .

As an example, as shown in FIG. 2 and FIG. 3 , the above-mentioned sensing part 140 may include a plug 141 that is detachably combined with the above-mentioned housing 110, and the above-mentioned plug 141 may include at least A combination hole 142 .

Wherein, the plug 141 is detachably coupled to the fastening hole 117 formed through the cover member 112 , and the sensors 143 , 144 are detachably coupled to the coupling hole 142 .

Meanwhile, the above-mentioned sensors 143, 144 may be connected with a controller (not shown) through cables.

Thus, the user can monitor and manage the state of the culture medium based on the information measured by the sensors 143 and 144 .

However, the type of the sensor is not limited thereto, and various known sensors can be used as long as they can measure information on the state of the medium required for culturing cells.

On the other hand, as described above, the air bubble removal device 100 according to an embodiment of the present invention can be applied to the medium circulation type cell culture system 1000 .

As an example, as shown in FIG. 5 , a medium circulation cell culture system 1000 according to an embodiment of the present invention may include a cell culture device 400 , a medium supply device 300 , a cell supply source 200 , an air bubble removal device 100 and a pump 500 .

Wherein, at least one support (not shown) to which cells to be cultured are attached may be provided inside the cell culture apparatus 400, and the medium supply apparatus 300 may store a predetermined amount of culture medium required for culturing cells. The above-mentioned cell supply source 200 may store a predetermined amount of inoculation medium required for culturing cells.

In addition, the cell supply source 200 can move the culture medium for inoculation stored inside to the side of the air bubble removal device 100 by injecting gas having a predetermined carbon dioxide concentration, and the culture medium can be stored in the medium supply device 300 Maintain the prescribed pH concentration required for culturing cells.

In this case, the cell culture device 400 , the medium supply device 300 , the cell supply source 200 , the air bubble removal device 100 , and the pump 500 may be connected via a plurality of connecting pipes 810 , 820 , 830 , 840 , and 850 .

In addition, the inoculation medium stored in the cell supply source 200 or the culture medium stored in the medium supply device 300 can be selectively supplied to the air bubble removal device 100 by opening and closing at least one valve 600 , 700 .

As an example, the culture medium stored in the medium supply device 300 can be sequentially circulated through the medium supply device 300, the air bubble removal device 100 and Cell culture device 400 .

In this case, the culture medium may be supplied from the medium supply device 300 to the air bubble removal device 100, and the culture medium flowing into the air bubble removal device 100 may be debubbled by the air bubble removal device 100, It is supplied to the above-mentioned cell culture apparatus 400 side. Simultaneously, the culture medium moved to the cell culture apparatus 400 can be recovered to the medium supply apparatus 300 and resupplied to the air bubble removal apparatus 100 in the state of returning to a predetermined pH concentration required for culturing cells.

Differently, the inoculation medium stored in the cell supply source 200 can be supplied to the air bubble removal device 100 by opening and closing the at least one valve 600 , 700 .

In this case, the medium for inoculation may be supplied to the side of the cell culture apparatus 400 after being moved to the air bubble removal apparatus 100 by the injection pressure supplied from the cell supply source 200 .

Accordingly, the inoculation medium can be supplied to the side of the cell culture apparatus 400 in a state where air bubbles have been removed by the air bubble removal apparatus 100 .

Thereby, the cells included in the above-mentioned inoculation medium can smoothly adhere to the support body side accommodated in the above-mentioned cell culture apparatus 400, and the inoculation medium moved to the above-mentioned cell culture apparatus 400 does not flow to the above-mentioned cell culture apparatus 400. The medium supply device 300 moves sideways and can stay in the cell culture device 400 .

Therefore, the cells included in the inoculation medium supplied from the air bubble removal device 100 to the cell culture device 400 can smoothly adhere to the surface of the support in the cell culture device 400 .

In the drawings and description, it is shown and explained that the above-mentioned air bubble removal device 100 is applicable to a circulation system that circulates a medium, but the present invention is not limited thereto, and may be applied to a static cell culture system that does not circulate a medium. As an example, the above-described air bubble removal device 100 can be applied to a cell seeding step or a first injection of a medium in a static cell culture system.

Meanwhile, cells cultured in the above-described cell culture device 300 may be utilized as cells for producing a large amount of artificial nanovesicles. That is, cells cultured in the above-described cell culture device 300 can be transformed into artificial nanovesicles by cell disruption.

Above, an embodiment of the present invention has been described, but the idea of the present invention is not limited to the embodiment proposed in this description, and those of ordinary skill in the technical field of the present invention who understand the idea of the present invention can use additional methods within the scope of the same idea. It is easy to propose other embodiments, such as changing, deleting, and adding structural elements, but this also falls within the scope of the idea of the present invention.

Claims (13)

1. An air bubble removal device for removing air bubbles from a culture medium, comprising: The casing includes an inner space, an inflow port, and an outflow port. The inner space is used to store a predetermined amount of the culture medium, the inflow port allows the culture medium to flow into the inner space from the outside, and the outflow port opens to the outside in the inner space. Discharging the culture medium in a state where air bubbles have been removed; and a support part is combined with the above-mentioned casing so that the above-mentioned casing can be maintained in a state separated from the bottom by a predetermined height, The above interior spaces include: The first space is filled with a prescribed amount of the above-mentioned culture medium; The second space is located on the upper part of the above-mentioned first space and is not filled with the above-mentioned culture medium, The first space and the second space are classified according to the water level of the culture medium that flows into the internal space from the outside through the inflow port. 2. The air bubble removal device for removing air bubbles from a culture medium according to claim 1, wherein The above-mentioned inflow port is provided in the above-mentioned housing so as to be located relatively higher than the above-mentioned outflow port, The outflow port is provided in the housing so as to be located at a position communicating with the first space, The inlet is provided in the casing so as to be located at a position communicating with the second space. 3. The air bubble removal device for removing air bubbles from a culture medium according to claim 1, wherein the second space is a space where the culture medium flowing into the inner space through the inflow port flows into the first space. Fall, and separate air bubbles in the bubble separation space. 4. The air bubble removal device for removing air bubbles from a culture medium according to claim 1, wherein the outflow port is provided in the housing so as to be located on the bottom surface of the internal space. 5. The air bubble removal device for removing air bubbles from the culture medium according to claim 1, characterized in that, the above-mentioned air bubble removal device for removing air bubbles from the culture medium also includes an exhaust port, and the above-mentioned exhaust port is arranged on the above-mentioned The shell is in order to communicate with the above-mentioned second space. 6. The air bubble removal device for removing air bubbles from a culture medium according to claim 5, wherein the air bubble removal device for removing air bubbles from a culture medium further comprises a pressure adjustment unit, and the pressure adjustment unit is connected with the above-mentioned exhaust air. The air ports are combined so that the internal pressure of the internal space can be adjusted to adjust the amount of the medium stored in the internal space. 7. The air bubble removal device for removing air bubbles from a culture medium according to claim 1, wherein said housing comprises: A box-shaped shell body having the above-mentioned internal space; The cover member is detachably coupled to the housing body so as to cover an open upper portion of the internal space. 8. The air bubble removal device for removing air bubbles from a culture medium according to claim 1, characterized in that, the above-mentioned air bubble removal device for removing air bubbles from a culture medium further comprises a sensing part, and the above-mentioned sensing part includes a A sensor that detects a state of a predetermined amount of the medium stored in the internal space. 9. The air bubble removal device for removing air bubbles from a culture medium according to claim 8, wherein the sensor includes a pH sensor for detecting the pH of the culture medium and a temperature sensor for measuring the temperature of the culture medium. at least one of the sensors. 10 . The air bubble removal device for removing air bubbles from a medium according to claim 8 , wherein the sensor is configured such that a part of its length including one end is immersed in the medium filled in the first space. 11 . 11. The air bubble removal device for removing air bubbles from a culture medium according to claim 8, wherein the sensing unit is detachably coupled to one side of the housing. 12. The air bubble removing device for removing air bubbles from a culture medium according to claim 1, wherein the culture medium is an inoculation medium including cells to be cultured or a culture medium including nutrient components. 13. A culture medium circulating cell culture system, characterized in that, comprising: A cell culture device provided with at least one support to which cells to be cultured are attached; a culture medium supply device internally storing a predetermined amount of a culture medium for culturing the above-mentioned cells; a cell supply source storing a prescribed amount of a culture medium for inoculation including cells to be attached to the above-mentioned support; A bubble removing device for supplying to one side of the cell culture device after removing bubbles in the culture medium flowing in from the medium supply device or the inoculation medium flowing in from the cell supply source; and a pump for circulating the above culture medium, The air bubble removal device is the air bubble removal device for removing air bubbles from a medium according to any one of claims 1 to 12.