CN116516575B - Curcumin-resveratrol protein-based nanofiber membrane and its preparation method and application - Google Patents

Abstract

The invention discloses a curcumin-resveratrol protein-based nanofiber membrane and its preparation method and application, and relates to the field of nanofiber materials. The curcumin-resveratrol protein-based nanofiber membrane proposed by the present invention uses soy protein isolate and zein for mixed spinning, and prepares two proteins with different solubility to obtain a stable protein in the form of a nanofiber membrane. base aggregate. Nanofiber membranes SPI+25%ZCR, 25%Z+25%ZCR, and 30%Z+30%ZCR made by coaxial electrospinning show excellent free radical scavenging capabilities, and SPI+25%ZCR It is more excellent and has more significant antibacterial effect. It also shows a slow and stable release rate when simulating in vitro release, and its performance is even better, ensuring that the soy protein isolate fiber film has broad application prospects in treating wounds.

Description

Curcumin-resveratrol protein-based nanofiber membrane and its preparation method and application

Technical field

The present invention relates to the field of nanofiber materials, and in particular to curcumin-resveratrol protein-based nanofiber membranes and preparation methods and applications thereof.

Background technique

Curcumin (Cur, abbreviated as C in the present invention) is an extremely active polyphenolic compound, which comes from the roots of turmeric and has an extremely low molecular weight. It has many biological activities such as anti-inflammatory, antioxidant, anti-tumor and anti-angiogenesis. And curcumin is very safe for humans and non-toxic even at high doses. However, like many other hydrophobic active substances, curcumin also has some problems that seriously affect its practical application, such as poor water solubility, unstable chemical structure, and low oral bioavailability, resulting in its use in food and Applications in the pharmaceutical industry are limited.

As a natural bioactive carrier, food protein is an ideal material for protein-based nanosystems. Composite nanoparticles prepared from food-grade materials have been widely used to encapsulate and deliver bioactive substances. Soy protein isolate (Spi) is the most widely used plant protein in the food industry. It has high acceptability and has been used to load active substances such as β-carotenoids, vanillin, and resveratrol. The loading rate of each active substance depends largely on the nature of the protein. It can be seen that the structure of soy protein isolate greatly affects the formation of protein-curcumin particles. Soy protein isolate has a significant composition of hydrophobic amino acids, polar groups and charged groups, so it can effectively promote the activity of small organisms through hydrophobic interactions, hydrogen bonding interactions and electrostatic interactions.

Due to the renewable properties of soy protein isolates, they have a wide range of sources, are rich in nutrients, are affordable, and have excellent processing properties, such as film-forming properties, air barrier properties, etc., making the materials prepared from them extremely biocompatible properties and degradability, thus bringing huge development potential to the food packaging and medical fields. However, because soy protein isolate also contains a large number of hydrophilic groups, the protein material is extremely hydrophilic, resulting in defects such as easy degradation, weak water resistance, and low mechanical strength.

The nanodelivery system of zein (Zein, abbreviated as Z in the present invention) is one of the current hot research directions. Zein is one of the by-products of corn processing and accounts for about half of the protein content of corn kernels. The protein is hydrophobic and can be dissolved in 60% to 90% ethanol aqueous solution. The isoelectric point (IEP) of Zein is 6.2. When the pH of the solution is lower than the IEP, Zein is positively charged. In contrast, anionic polysaccharides become negatively charged when the pH of the solution is higher than their own pKa. Therefore, within a specific pH range, they can have opposite charges, forming complexes through electrostatic interactions. However, zein nanoparticles have poor aggregation stability when exposed to certain environmental conditions such as pH near the isoelectric point, high salt levels, and elevated temperatures.

Protein fiber films prepared by electrospinning technology have extremely high specific surface area and porosity, which makes them easier to decompose and degrade under physical and chemical conditions, thus providing great potential for applications in the field of wound dressings.

In addition, current combinations containing multiple bioactive ingredients have better stability or bioactivity, while single components are poorer. For example, co-encapsulation of Res and tocopherol (α-TOC) in emulsions not only improves α -TOC's chemical stability and bioavailability have also been improved to a certain extent. Resveratrol (Res, abbreviated as R in the present invention), 3-5-4'-trihydroxy-stilbene, is a non-flavonoid polyphenol compound with potential health benefits, including anti-cancer, inflammation, anti-obesity and protective effects on the heart and brain. Therefore, the development of a variety of different bioactive ingredients can yield important social and economic benefits.

The present invention prepares zein-based nanofiber membranes and soy protein isolate-based nanofiber membranes co-embedding curcumin and resveratrol through coaxial electrospinning technology, and analyzes the microscopic properties of the prepared nanofiber membranes. The structure, morphology, mechanical properties, thermal properties and functional properties were characterized and analyzed to explore the effects of different protein bases on the properties of nanofiber membranes and provide feasible solutions for applications in the field of active packaging.

Contents of the invention

The purpose of the present invention is to propose a curcumin-resveratrol protein-based nanofiber membrane and its preparation method and application.

In order to achieve the above objects, the technical solutions of the present invention are as follows:

In the first aspect, the present invention proposes a method for preparing a curcumin-resveratrol protein-based nanofiber membrane, using a mixed solution of soy protein isolate and polyethylene oxide as the shell liquid, and adding curcumin and resveratrol to zein alcohol. The protein-soluble solution was used as the core liquid, and the shell liquid and core liquid were coaxially electrospun to obtain curcumin-resveratrol protein-based nanofiber membranes.

Preferably, the above-mentioned preparation method of curcumin-resveratrol protein-based nanofiber membrane includes the following steps:

S1. Use 85% ethanol aqueous solution as the solvent to prepare 25 wt% soy protein isolate solution and 13 wt% polyoxyethylene solution respectively. Add the 25 wt% soy protein isolate solution and 13 wt% polyoxyethylene solution according to the Mix at a mass ratio of 1:1 and stir until the solution is evenly mixed and used as shell liquid;

S2. Prepare a 25 wt% zein solution, add curcumin and resveratrol to it, the added amounts of curcumin and resveratrol are both 10 mg/ml, and stir until the solution is evenly mixed to serve as a nuclear liquid;

S3. Perform coaxial electrospinning of the shell liquid and core liquid to obtain a curcumin-resveratrol protein-based nanofiber membrane, recorded as 25%Z+25%ZCR.

Preferably, the above-mentioned preparation method of curcumin-resveratrol protein-based nanofiber membrane includes the following steps:

S1. Use 85% ethanol aqueous solution as the solvent to prepare 30 wt% soy protein isolate solution and 13 wt% polyoxyethylene solution respectively. Add the 30 wt% soy protein isolate solution and 13 wt% polyoxyethylene solution according to the Mix at a mass ratio of 1:1 and stir until the solution is evenly mixed and used as shell liquid;

S2. Prepare a 30 wt% zein solution, add curcumin and resveratrol to it, the added amounts of curcumin and resveratrol are both 10 mg/ml, and stir until the solution is evenly mixed to serve as a core liquid;

S3. Perform coaxial electrospinning of the shell liquid and core liquid to obtain a curcumin-resveratrol protein-based nanofiber membrane, recorded as 30%Z+30%ZCR.

Preferably, the above-mentioned preparation method of curcumin-resveratrol protein-based nanofiber membrane includes the following steps:

S1. Use hexafluoroisopropanol as the solvent to prepare a solution with a mass ratio of soy protein isolate:polyethylene oxide of 4:1. Stir ultrasonically to fully dissolve the solution as a shell liquid, which is recorded as SPI;

S2. Prepare a 25 wt% zein solution, add curcumin and resveratrol to it, the added amounts of curcumin and resveratrol are both 10 mg/ml, and stir until the solution is evenly mixed to serve as a nuclear liquid;

S3. Perform coaxial electrospinning of the shell liquid and core liquid to obtain a curcumin-resveratrol protein-based nanofiber membrane, which is recorded as SPI+25%ZCR.

Preferably, in the above-mentioned preparation method of curcumin-resveratrol protein-based nanofiber membrane, in step S1, the mass ratio of soy protein isolate and polyethylene oxide is 4:1.

Preferably, in the above-mentioned preparation method of curcumin-resveratrol protein-based nanofiber membrane, the parameters of coaxial electrospinning are set to: shell liquid flow rate 5 mL/h, core liquid flow rate 2 mL/h, spinning time 1 h, voltage 25 kv, rotation speed 1500 r/min, receiving distance 13 cm, ambient temperature 25±2℃, humidity 50±5%.

In a second aspect, the present invention also proposes a curcumin-resveratrol protein-based nanofiber membrane, which is prepared by any of the above methods.

In a third aspect, the present invention also proposes the application of curcumin-resveratrol protein-based nanofiber membrane in wound dressings.

Compared with the existing technology, the technical effects of the present invention are:

The curcumin-resveratrol protein-based nanofiber membrane proposed by the present invention uses soy protein isolate and zein for mixed spinning, and prepares two proteins with different solubility to obtain a stable protein in the form of a nanofiber membrane. base aggregate. Nanofiber membranes SPI+25%ZCR, 25%Z+25%ZCR, and 30%Z+30%ZCR made by coaxial electrospinning show excellent free radical scavenging capabilities, and SPI+25%ZCR It is more excellent and has more significant antibacterial effect. It also shows a slow and stable release rate when simulating in vitro release, and its performance is even better, ensuring that the soy protein isolate fiber film has broad application prospects in treating wounds.

Description of drawings

Figure 1 is a scanning electron microscope image of a nanofiber membrane under uniaxial conditions provided by an embodiment of the present invention. In the figure, A is a fiber membrane made of soy protein isolate and polyethylene oxide with a mass ratio of 4:1, recorded as SPI, and B is 25%Z.

Figure 2 is a scanning electron microscope image of the nanofiber membrane under coaxial conditions provided by the embodiment of the present invention. In the figure, C is 25%Z+25%ZCR, D is 30%Z+30%ZCR, and E is SPI+25%ZCR.

Figure 3 is a physical diagram of the nanofiber membrane provided by the embodiment of the present invention. In the figure, F is 25%Z+25%ZCR, G is SPI+25%ZCR loaded with curcumin and resveratrol, and H and I are SPI.

Figure 4 is a Fourier transform infrared spectrum chart of different nanofiber membranes provided by embodiments of the present invention.

Figure 5 is a DSC curve chart of different nanofiber membranes provided by embodiments of the present invention.

Figure 6 shows the oxidation resistance results of the nanofiber membrane provided by the embodiment of the present invention.

Figure 7 shows the antibacterial results of the nanofiber membrane provided by the embodiment of the present invention.

Figure 8 shows the in vitro release results of the nanofiber membrane provided by the embodiment of the present invention.

Detailed ways

In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be described in further detail below with reference to the embodiments and drawings. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

The experimental methods in the following examples are all conventional methods unless otherwise specified.

The test materials used in the following examples were all purchased from conventional biochemical reagent stores unless otherwise specified. Prolamin was purchased from Wako, Japan. Soy protein isolate was purchased from Shanghai Yuanye Biotechnology Co., Ltd. Curcumin was purchased from Beijing Bailingwei Company. Resveratrol was purchased from McLean.

1. Preparation of spinning solution

(1) Use hexafluoroisopropyl alcohol as the solvent, mix soy protein isolate (Spi) and polyethylene oxide (PEO) according to the mass ratio of 1:1, 2:1, 4:1, 8:1, and mix at 45 ℃ for 1 day under ultrasonic conditions to fully dissolve the solution, and perform uniaxial electrospinning on them respectively to obtain the best soy protein isolate nanofiber membrane (the mass ratio of Spi to PEO is 4:1), which is recorded as SPI.

(2) Using 85% ethanol aqueous solution as the solvent, prepare the following solutions: a. 25 wt% zein (Zein) solution and 13 wt% PEO solution; b. 30 wt% zein (Zein) solution and 13 wt% PEO solution; c. Add curcumin and resveratrol ethanol solution (10 mg/ml) to 25 wt% Zein solution and 13 wt% PEO solution; d. Add curcumin and resveratrol Alcoholic ethanolic solution (10 mg/ml) of 30 wt% Zein and 13 wt% PEO. Mix according to the mass ratio of Zein:PEO 1:1, stir at room temperature for 2 h for uniaxial electrospinning, and the obtained nanofiber membranes are recorded as 25%Z, 30%Z, 25%ZCR and 30%ZCR respectively.

(3) Using 85% ethanol aqueous solution as the solvent, prepare a 25 wt% Zein solution and a 13 wt% PEO solution. Mix according to the mass ratio of 1:1 and stir at room temperature for 30 min to form a shell liquid. Prepare a 25 wt% Zein solution, add curcumin and resveratrol (10 mg/ml) to it, mix and stir for 5 minutes at room temperature as the core liquid for coaxial electrospinning, and the resulting nanofiber membrane is recorded as 25%Z+ 25%ZCR.

(4) Using 85% ethanol aqueous solution as the solvent, prepare a 30 wt% Zein solution and a 13 wt% PEO solution. Mix according to the mass ratio of 1:1 and stir at room temperature for 30 min to form a shell liquid. Prepare a 30 wt% Zein solution, add curcumin and resveratrol (10 mg/ml), mix and stir at room temperature for 5 minutes as the core liquid for coaxial electrospinning, and the resulting nanofiber membrane is recorded as 30%Z+ 30%ZCR.

(5) Use hexafluoroisopropanol as the solvent to prepare a solution with a Spi:PEO mass ratio of 4:1, and stir it thoroughly for 1 day at 45°C under ultrasonic conditions to serve as a shell liquid. Prepare a 25 wt% Zein solution, add curcumin and resveratrol (10 mg/ml) to it, mix and stir for 5 minutes at room temperature as the core liquid for coaxial electrospinning, and the resulting nanofiber membrane is recorded as SPI+25% ZCR.

2. Spinning parameters

The parameters of uniaxial electrospinning were set as flow rate 1.5 mL/h, spinning time 2 h, voltage 25 kv, rotation speed 1500 r/min, receiving distance 10 cm, ambient temperature 25±2°C, and humidity 50±5%.

The parameters of coaxial electrospinning are set as shell liquid flow rate 5 mL/h, core liquid flow rate 2 mL/h, spinning time 1 h, voltage 25 kv, rotation speed 1500 r/min, receiving distance 13 cm, and ambient temperature 25 ±2℃, humidity 50±5%.

3. Scanning electron microscope

The fiber membrane is sprayed with gold to enhance conductivity and obtain a clearer micromorphology. After gold spraying, the micromorphology of the nanofiber membrane was observed by field emission environmental scanning electron microscopy.

The morphology images of SPI and Zein nanofiber membranes prepared by uniaxial electrospinning are shown in Figure 1, A and B, respectively. A is SPI, B is 25%Z. The nanofibers obtained by electrospinning with two proteins, SPI and Zein, were continuous, bead-free and inhomogeneous cylindrical shapes.

The morphology images of 25%Z+25%ZCR, 30% Z+30%ZCR and SPI+25%ZCR nanofiber membranes prepared by coaxial electrospinning are shown in Figure 2 C, D and E respectively. The nanofibers obtained by electrospinning with different proteins as carriers are all in the shape of continuous, bead-free ribbons. Among them, 25% Z+25% ZCR and 30% Z+30% ZCR nanofibers prepared by coaxial electrospinning The distribution of fiber membranes is relatively uniform, and the SPI+25%ZCR nanofiber membrane is the most uniformly distributed, showing a continuous, slender and uninterrupted ribbon shape, and the surface is smoother and the fiber diameter distribution is more uniform. The 25%Z+25%ZCR nanofibers are slightly entangled, and the fiber diameter distribution is uneven, which may be caused by the low Zein concentration.

As shown in Figure 3, the 25%Z+25%ZCR nanofiber membrane is slightly adherent to the tinfoil, and is difficult to peel off from the tinfoil. The nanofiber membrane appears light and thin like silk. The SPI nanofiber film is not easy to peel off from the tinfoil, and appears as a white, thin strip after peeling off. The SPI+25%ZCR nanofiber membrane is easy to peel off from the received tin foil, and the resulting nanofiber membrane looks like paper. Compared with SPI+25%ZCR blend, the nanofiber membrane produced by SPI has poor mechanical properties and is not suitable for use as an active packaging film. At the same time, in order to achieve better spinning effect, the prepared nanofiber membrane is made of SPI+25% ZCR and becomes a more stable food packaging material.

4. Measurement of Fourier transform infrared spectrum

Mix the nanofiber membrane and KBr at a ratio of 1:100, grind the sample and KBr into powder in a mortar, press the film for 1 minute, and then put it into the instrument for measurement. The scanning range is 4000~400 cm ^-1 , the resolution is 4 cm ^-1 , and Fourier transform infrared spectrum measurement is performed under 32 scanning conditions. During the measurement, potassium bromide tablets were used as a blank control, and the obtained infrared spectrum was analyzed using Origin software.

By measuring the Fourier transform infrared spectrum of the nanofiber membrane, it can be determined whether the coaxial nanofiber membrane with Zein and PEO as the core and soy protein isolate as the shell has been successfully prepared, and whether curcumin and resveratrol have been successfully loaded on the nanofibers. in the membrane. As shown in Figure 4, the characteristic peaks of the 25%Z nanofiber membrane are at 3415 cm ^-1 , 1639 cm ^-1 and 1544 cm ^-1 . When at 3415 cm ^-1 , the stretching vibration of OH can be observed, while at 1639 cm ^-1 , the stretching of C=O can be observed, and when at 1544 cm ^-1 , the stretching of CN can be observed Stretch and NH bending vibration. The characteristic peaks of SPI are at 3409 cm ^-1 and 1650 cm ^-1 . When it is at 3409 cm ^-1 , the stretching vibration of OH can be observed, and when it is at 1650 cm ^-1 , the stretching of C=O can be observed. When curcumin and resveratrol were loaded, the OH peaks of 25% ZCR and SPI+25% ZCR moved to 3388 and 3335 cm ^-1 . The characteristic peaks of curcumin at 1601 cm ^-1 and 1205 cm ^-1 and the characteristic peaks of resveratrol (Res) at 1383 cm ^-1 and 1152 cm ^-1 are reduced or even disappeared in the nanofiber membrane formed by electrospinning. This may be It has something to do with it being wrapped. The peak of 25%Z at 3415 cm ^-1 shifted in the nanofiber membrane after loading polyphenols, which indicated that polyphenols were present in the fiber membrane.

5. Determination of thermal stability

Differential scanning calorimetry (DSC) was used to measure nanofiber films prepared by uniaxial and coaxial electrospinning. Take a sample of 4 ~ 5 mg in an aluminum pan, cover it and put it into DSC. Use an empty aluminum pan as a control. Test conditions: nitrogen atmosphere, flow rate 50 mL/min, and then rising from 20°C to 250°C at a heating rate of 10°C/min. After the test is completed, use the analysis software that comes with the TA instrument to analyze the thermal stability of the sample.

As shown in Figure 5, there is an obvious endothermic peak on the DSC curve of each nanofiber membrane, which is the thermal denaturation temperature (Td) of the nanofiber membrane. The Td values of uniaxial electrospun nanofiber membranes SPI and 30%Z are 113.07℃ and 117.08℃ respectively. The Td values of uniaxial nanofiber membranes 30%ZCR loaded with Cur+Res increase to 118.51℃ and 25%Z. The Td values of +25%ZCR and 30%Z+30%ZCR are close. When the shell solution was changed from Zein to SPI, the Td value dropped significantly, by 11.59%. The lower the Td, the less heat is required to destroy the membrane structure. It is speculated that the decrease in Td value may be due to the fact that the energy of the interaction between SPI and Zein is lower than the energy of Zein coaxial electrospinning, which reduces the ordered structure of zein, resulting in a change in the structure of the nanofiber membrane.

6. Determination of antioxidant properties

Two free radical scavenging methods, DPPH and ABTS, were used to examine the antioxidant properties of different coaxial nanofiber membranes. Weigh 10 mg of the nanofiber membrane and shake it in 1 mL of 85% ethanol aqueous solution at room temperature for 1 h.

Take the sample supernatant and mix it with 0.1 mM DPPH in a ratio of 1:30, and react in the dark for 15 minutes. Record the absorbance at 517 nm using a spectrophotometer.

Mix ABTS (water-soluble 7 mM) and potassium persulfate (water-soluble 2.45 mM) at a ratio of 1:1 and place at room temperature in the dark for 12 h. Mix the sample supernatant and ABTS diluent at a ratio of 1:20 and react in the dark for 15 minutes. Record the absorbance at 734 nm using a spectrophotometer.

DPPH and ABTS solutions without adding nanofiber membrane were used as blank controls respectively. Three parallel groups were made for each sample, and the free radical scavenging ability was calculated using the following formula:

Among them, Ac is the absorbance value of the reference substance, and As is the absorbance value of the sample.

The calculation results are shown in Figure 6. The results show that the nanofiber membrane containing only SPI has a scavenging rate of DPPH free radicals of 19.99%, and a relatively low scavenging rate of ABTS free radicals, showing weak antioxidant properties. The 30% Z nanofiber membrane has strong antioxidant capacity and can effectively scavenge DPPH free radicals with a scavenging rate of up to 16.01%, while the ABTS free radical scavenging rate has reached 60.63%. This is due to Zein's own antioxidant properties. 30%ZCR shows excellent free radical scavenging ability. At the same time, the nanofiber membranes SPI+25%ZCR, 25Z+25%ZCR, and 30%Z+30%ZCR made by coaxial electrospinning also show relatively good performance. Excellent free radical scavenging ability, and SPI+25% ZCR is even better, ensuring that the soy protein isolate fiber film has broad application prospects in treating wounds.

7. Determination of antibacterial properties

The agar plate diffusion method was used to evaluate the antibacterial performance of the nanofiber membrane against Staphylococcus aureus (purchased from China General Microbial Culture Collection and Management Center, preservation number CGMCC1.1861). Take 100 μL of bacterial liquid and spread it evenly on the Luria broth (LB) agar medium of the plate to culture Staphylococcus aureus. Use a hole punch to make the nanofiber membrane into a disc with a diameter of 6 mm, and irradiate it under a UV lamp for 30 minutes to achieve disinfection before use. The sterilized nanofiber membrane was adhered to the agar medium coating of the plate and incubated in a 37°C incubator for 24 h. The antibacterial performance of the nanofiber membrane was evaluated by the size of the inhibition zone.

The results are shown in Figure 7. When the added amounts of resveratrol and curcumin were 0.0%, no inhibition zone appeared near the nanofiber membrane, indicating that SPI and 25% Z did not have antibacterial properties. When 2.0% Cur and 2.0% Res were added to it, an obvious inhibition zone appeared near the SPI+25% ZCR nanofiber membrane, indicating that the nanofiber membrane loaded with Cur and Res has an inhibitory effect on Staphylococcus aureus, and In the present invention, the antibacterial effect of the nanofiber membrane SPI+25%ZCR is more obvious.

8. Determination of in vitro release

In this experiment, 10% ethanol aqueous solution was used as a food simulation liquid to study the in vitro release of the designated nanofiber membrane in the food simulation liquid in this environment. Weigh 10 mg of nanofiber membrane, immerse it in 10 mL of food simulation solution, and store at 25°C. Centrifuge (2000 rpm 2 min) at certain time intervals (0.5 h, 1 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h). After centrifugation, take out 1 mL of the supernatant and use visible spectrophotometry. Measure the absorbance at 330nm with a meter respectively, and make up the simulated solution. Calculate the concentration of Cur and Res released from the fiber membrane in the food simulation solution by drawing a standard curve. Each fiber membrane was tested three times, and the results were averaged.

The release results of the nanofiber membrane in food simulation liquid are shown in Figure 8. In the initial stage, burst release of various nanofiber membranes occurred in the food simulation liquid. Among them, 30% ZCR released the most in the initial stage, releasing 79.36% in the food sustained-release solution in the first 2 hours, and then showed a long-term sustained slow release. At 4 h, the release percentage of 25%Z+25%ZCR and 30%Z+30%ZCR in the food simulation solution was about 60%, and then also showed a sustained slow release for a long time. The release of curcumin from SPI+25% ZCR in the food environment gradually increased with time, showing the highest release rate of 87.11% at 12 hours.

Based on the above experimental results, we draw the following conclusions:

(1) Zein-based nanofiber membranes prepared by uniaxial and biaxial electrospinning technology have different core-shell structures. After SEM technology inspection, it was found that the surface of the nanofiber membrane prepared by SPI-Zein protein-based aggregates Smooth, evenly distributed in diameter, and has good mechanical properties.

(2) The successful loading of polyphenols in the nanofiber membrane was confirmed by FTIR. The peak of 25%Z at 3415 cm ^-1 shifted in the nanofiber membrane after loading polyphenols, which indicated that polyphenols were present in the fiber membrane.

(3) By analyzing the thermal properties of different nanofiber membranes, it was found that when the shell solution was changed from Zein to SPI, the Td value dropped significantly, by 11.59%, and less heat was required when the membrane structure was destroyed.

(4) Polyphenol-loaded nanofiber membranes have antioxidant and Staphylococcus aureus inhibitory effects. Nanofiber membranes containing only SPI and nanofiber membranes containing only Zein have certain antioxidant properties, but their antioxidant properties are weak. The nanofiber membranes SPI+25%ZCR, 25%Z+25%ZCR, and 30%Z+30%ZCR made by coaxial electrospinning show relatively excellent free radical scavenging capabilities, and SPI+25% ZCR is more excellent and has more significant antibacterial effect. It also shows a slow and stable release rate when simulating in vitro release, and its performance is even better.

Certain exemplary embodiments of the present invention have been described above only by way of illustration. It goes without saying that those skilled in the art can implement various embodiments in various ways without departing from the spirit and scope of the present invention. The described embodiments are modified. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of the claims of the present invention.

Claims (4)

1. Preparation method of curcumin-resveratrol protein-based nanofiber membrane, characterized in that a mixed solution of soy protein isolate and polyethylene oxide is used as the shell liquid, and zein with curcumin and resveratrol is added The solution is used as the core liquid, and the shell liquid and the core liquid are coaxially electrospun to obtain a curcumin-resveratrol protein-based nanofiber membrane; the method includes the following steps: S1. Use hexafluoroisopropanol as the solvent to prepare a solution with a mass ratio of soy protein isolate:polyethylene oxide of 4:1. Stir ultrasonically to fully dissolve the solution as a shell liquid, which is recorded as SPI; S2. Prepare a 25 wt% zein solution, add curcumin and resveratrol to it, the added amounts of curcumin and resveratrol are both 10 mg/ml, and stir until the solution is evenly mixed to serve as a nuclear liquid; S3. Perform coaxial electrospinning of the shell liquid and core liquid to obtain a curcumin-resveratrol protein-based nanofiber membrane, which is recorded as SPI+25%ZCR. 2. The preparation method of curcumin-resveratrol protein-based nanofiber membrane according to claim 1, characterized in that the parameters of coaxial electrospinning are set to: shell liquid flow rate 5 mL/h, core liquid flow rate 2 mL/h, spinning time 1 h, voltage 25kv, rotation speed 1500 r/min, receiving distance 13 cm, ambient temperature 25±2℃, humidity 50±5%. 3. Curcumin-resveratrol protein-based nanofiber membrane, characterized in that it is made by the method described in any one of claims 1 to 2. 4. Application of the curcumin-resveratrol protein-based nanofiber membrane according to claim 3 in wound dressings.