CN116474652A - Synthetic board waste liquid discharge device and gene synthesizer - Google Patents

Abstract

The present invention relates to the technical field of gene synthesis, in particular to a synthetic plate waste liquid discharge device, comprising a liquid pumping plate, the liquid pumping plate is used to be installed at the bottom of the synthetic plate, the liquid pumping plate includes a drain tank with an open upper end, and the lower ports of the synthetic holes of the synthetic plate are all connected to the drain tank, and the drain tanks are separated to form at least two mutually independent sub-troughs with open upper ends, wherein each sub-groove is connected to a drain channel, and each drain channel is provided with a valve for controlling the opening/closing of the drain channel; the discharge device also includes a negative pressure unit, the negative pressure unit communicates with the drainage channel for applying negative pressure to the drainage channel; in the assembled state, the synthesis holes on the synthesis plate are divided into at least two groups, and one group of synthesis holes corresponds to a sub-slot; during the liquid drainage process, the normal liquid addition operation can still be performed; and the sub-slots can independently pump liquid, without interfering with the normal synthesis of the synthesis hole groups corresponding to other sub-slots.

Description

A synthetic board waste liquid discharge device and a gene synthesizer

technical field

The invention relates to the technical field of gene synthesis, in particular to a synthetic board waste liquid discharge device and a gene synthesizer.

Background technique

Most of the DNA synthesizers currently on the market use the phosphorous acid amide chemical synthesis method for DNA synthesis, which is carried out in four steps: deprotection, activation coupling, capping, and oxidation. Different chemical reagents are required in each step. Among them, the deprotection reagent is mainly trichloroacetic acid solution (TCA), the four monomer reagents are T, G, C, A, the activation reagent is tetrazole (ACT), the capping reagent is CAPA and CAPB, the oxidation reagent is mainly iodine solution (Ox), and the cleaning reagent is mainly acetonitrile solution (ACN).

Among them, the synthesis work is mainly carried out in the synthesis plate of the synthesizer, that is, the reagent is added into the synthesis well of the synthesis plate from the liquid addition module and reacted on the synthesis column in the synthesis well to realize the synthesis.

Generally, after the synthesis is completed, the waste liquid in the synthesis hole needs to be discharged. Therefore, a drain tank is generally arranged at the bottom of the synthesis plate to discharge the waste liquid in the synthesis hole.

The traditional way of discharging waste liquid is to rely on the waste liquid to seep naturally into the drainage tank under gravity, and then to be discharged from the drainage tank. For details, please refer to a mobile pipeline drainage device applied to a multi-channel DNA synthesizer disclosed in the patent document with the publication number CN204198729U. Although this method can realize the discharge of waste liquid, it is obvious that the discharge speed is too slow only by relying on the natural seepage of waste liquid.

Therefore, the currently commonly used liquid discharge method is mainly positive pressure discharge. Specifically, when discharging waste liquid, the synthetic plate is in a closed space, and then pressurized into the closed space to form a positive pressure. At this time, the upper part of the synthetic plate (that is, the upper port of the synthetic hole) is in a positive pressure environment. Compress and seal the air blowing and liquid draining device.

Although the above-mentioned positive pressure method has improved the liquid discharge speed, there are still deficiencies, specifically:

First of all, when adding liquid, the nozzle of the liquid adding module is used to add liquid to the synthesis hole at the upper port position of the synthesis hole, and when the liquid is discharged, because the upper part of the synthesis plate is in a positive pressure environment, and in a positive pressure environment, the nozzle of the nozzle cannot eject the reagent normally, so when the liquid is discharged, the nozzle cannot normally add liquid. In other words, during the liquid discharge process, the liquid addition needs to be stopped.

Secondly, this positive pressure drainage method needs to wait for all the synthesis holes on the synthesis plate to complete the synthesis reaction before draining the liquid, because once the positive pressure is formed, the upper ports of all the synthesis holes will be in a positive pressure environment, and all the synthesis holes will start to drain at this time; but in the actual application process, due to the order of adding liquid first and then, and the synthesis reaction time of different reagents is different, so when some synthesis holes complete the reaction, some synthesis holes have not yet completed the synthesis. Liquid, so that this part of the synthesis hole cannot be synthesized normally.

Contents of the invention

In order to solve at least one technical problem mentioned in the background art, the object of the present invention is to provide a synthetic board waste liquid discharge device.

To achieve the above object, the present invention provides the following technical solutions:

A composite board waste liquid discharge device, comprising a liquid pumping plate, the liquid pumping board is used to be installed on the bottom of the composite board, the liquid pumping board includes a drain tank with an open upper end, and the lower ports of the synthetic holes of the composite board are all communicated with the drain tank, and the drain tank is separated to form at least two independent sub-grooves with open upper ends, wherein each sub-groove is connected to a drain channel, and each drain channel is provided with a valve for controlling the opening/closing of the drain channel; The channel communication is used to apply negative pressure to the drainage channel; in the assembled state, the synthetic holes on the synthetic plate are divided into at least two groups, and a group of synthetic holes corresponds to a sub-groove.

Compared with the prior art, the advantages of adopting this solution are:

In this solution, by setting the drainage tank and the negative pressure unit that applies negative pressure, it is equivalent to using negative pressure to realize the waste liquid discharge of the synthesis hole, that is, the negative pressure is applied by the negative pressure unit, so that the negative pressure is formed in the drainage tank. Positive pressure, so that it will not interfere with the normal liquid addition of the liquid addition module. In other words, in this solution, the normal liquid addition operation can still be performed during the liquid discharge process.

Secondly, in this solution, the method of negative pressure drainage is adopted, and the drainage tank is divided into at least two independent sub-slots, and the drainage channel of each sub-slot is equipped with a valve, so that after the composite plate and the pumping plate are assembled, a group of synthetic holes corresponds to a sub-slot, so that when in use, the sub-slot can be used as a sub-slot for partitioned drainage. Normal synthesis for the synthesis hole groups corresponding to other subslots.

Preferably, the at least two sub-slots are arranged side by side in sequence along the length direction of the drainage tank, wherein two adjacent sub-slots are separated by a partition extending along the width direction of the drainage tank.

Preferably, the discharge device further includes a first seal, which is used to be installed between the abutment surface of the liquid pumping plate and the composite plate, and the first seal surrounds the outer periphery of the drainage groove; and/or the discharge device also includes a second seal, which is used to be installed between the abutment surface of the composite plate and the partition, and it extends along the length direction of the partition.

Preferably, the top of the partition extends upwards from the top surface of the liquid pumping plate to form a protrusion, the bottom wall of the composite plate corresponds to the position of the partition and has a slot for the protrusion to be embedded in, and the second sealing member is arranged in the slot.

Preferably, the sub-groove includes a first end and a second end, and the bottom of the sub-groove is a slope structure gradually inclined downward from the second end to the first end, wherein the drainage channel communicates with the first end of the sub-groove.

Preferably, the drainage channel is provided at the end side of the sub-slot to communicate with the sub-slot, and in two adjacent sub-slots, the drainage channels of the two sub-slots are on opposite sides.

The present invention also provides a gene synthesizer, including a synthesis board, and a liquid addition module for adding liquid to the synthesis hole of the synthesis board. The synthesis board is connected with a waste liquid discharge device, and the waste liquid discharge device adopts the above-mentioned synthesis board waste liquid discharge device.

Preferably, it also includes a closed synthesis chamber, the synthesis plate is arranged horizontally in the synthesis chamber, and the synthesis chamber is provided with an air inlet for the protection gas to pass through and an air outlet for exhaust.

Preferably, it includes a bottom case and a cover plate, the cover plate can be opened and closed on the bottom case, the cover plate and the bottom case enclose the synthesis chamber in the closed state, and the liquid adding module is arranged on the cover plate.

Preferably, a linear module is provided in the synthesis chamber, and the synthesis plate and the liquid pumping plate are installed on the linear module and driven by the linear module to move horizontally and linearly; the linear module is also equipped with a liquid receiving plate, and the liquid receiving plate moves synchronously with the synthetic plate.

Other advantages and effects of the present invention are explained in detail in the specific embodiments.

Description of drawings

Fig. 1 is the schematic structural view of the liquid pumping plate of the present invention;

Fig. 2 is a schematic cross-sectional view of the assembled state of the liquid pumping plate and the composite plate of the present invention;

Fig. 3 is a partial enlarged view in Fig. 2;

Fig. 4 is a schematic diagram of the bottom structure of the synthetic board of the present invention;

Fig. 5 is a structural schematic diagram of the synthetic board installed in the state of the linear module in the present invention;

Fig. 6 is a schematic structural view of embodiment 2 under the state of the cover plate being closed;

Fig. 7 is a schematic structural view of embodiment 2 when the cover plate is opened;

FIG. 8 is a schematic structural view of the synthesis chamber in Example 2.

Detailed ways

The technical solutions of the embodiments of the present invention will be explained and described below in conjunction with the accompanying drawings of the embodiments of the present invention, but the following embodiments are only preferred embodiments of the present invention, not all of them. Based on the examples in the implementation manners, other examples obtained by those skilled in the art without making creative efforts all belong to the protection scope of the present invention.

In the following description, terms such as "inner", "outer", "upper", "lower", "left", "right", etc. appearing to indicate orientation or positional relationship are only for the convenience of describing the embodiment and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as limiting the present invention.

Example 1

Please refer to FIGS. 1-5 , this embodiment provides a synthesis plate waste liquid discharge device, which is mainly used in a gene synthesizer to discharge waste liquid in the synthesis hole 21 of the synthesis plate 2 .

For the convenience of understanding, this embodiment briefly describes the synthesis plate 2. Generally speaking, as shown in FIG. 2, the synthesis plate 2 is provided with a number of vertically penetrating synthesis holes 21, and the synthesis holes 21 are provided with synthesis columns (not shown in the figure). There are a lot of descriptions and applications of this composite board 2 in the prior art, so too many details will not be repeated here.

As shown in Fig. 1 , the composite plate waste liquid discharge device provided in this embodiment includes a liquid suction plate 1, which is used to be installed on the bottom of the composite plate 2;

As shown in Figure 1, the drainage groove 11 is separated to form at least two sub-grooves 11a that are independent of each other and open at the upper end, and the specific number of the sub-grooves 11a can be specifically designed according to the number of composite holes 21 on the composite plate 2, for example, in this embodiment, eight sub-grooves 11a are used.

It is worth noting that the sub-grooves 11a here are independent from each other, and it can also be understood that after the composite plate 2 is assembled with the liquid pumping plate 1, the sub-grooves 11a are closed to each other, and each sub-groove 11a is used as a separate closed space.

The specific composition and distribution of the sub-slots 11a in this embodiment are, as shown in Figure 1, taking a rectangular liquid drainage tank 11 as an example, the sub-slots 11a are arranged side by side along the length direction of the liquid drainage tank 11, wherein the adjacent two sub-slots 11a are separated by a partition 12 extending along the width direction of the liquid drainage tank 11, and the partition 12 makes the adjacent two sub-slots 11a become independent units.

In addition, it is worth noting that the above-mentioned length direction of the drainage groove 11 and the width direction of the drainage groove 11 are only a relative concept, and it is not absolutely required that the extending direction of the long side of the drainage groove 11 is the length direction, and the extending direction of the short side of the drainage groove 11 is the width direction. The direction in which the short sides of the groove 11 extend is the longitudinal direction.

Wherein each sub-tank 11a is connected with a drain channel 13, that is, one sub-trough 11a corresponds to a drain channel 13, and the drain channel 13 is mainly used to lead out the waste liquid in the corresponding sub-trough 11a, wherein the drain channel 13 can be a pipeline, such as a hose pipeline.

As shown in FIG. 5 , each discharge channel 13 is provided with a valve 14 that controls the opening/closing of the discharge channel 13 , that is, one discharge channel 13 corresponds to a valve 14 , wherein the valve 14 can be a solenoid valve, preferably a normally closed solenoid valve.

The discharge device also includes a negative pressure unit (not shown in the figure), which communicates with the drain channel 13 for applying negative pressure to the drain channel 13, thereby forming a negative pressure in the sub-tank 11a; in this embodiment, the negative pressure unit can be a negative pressure container with a negative pressure environment inside, such as a negative pressure tank; in other optional embodiments, the negative pressure unit can also be a vacuum pump capable of sucking air, etc., which are not specifically limited here.

In the assembled state, as shown in Figure 2 and Figure 5, the composite plate 2 is mounted on the top surface of the liquid pumping plate 1, and the synthetic holes 21 on the composite plate 2 are divided into at least two groups, and one group of synthetic holes 21 corresponds to one sub-groove 11a; taking 8 sub-grooves 11a as an example, the synthetic holes 21 on the composite plate 2 are divided into 8 groups according to the distribution direction of the 8 sub-grooves 11a, which is equivalent to the synthetic hole 21 forming 8 synthetic hole groups in total, and each synthetic hole 21 group The lower port of the middle synthetic hole 21 communicates with the corresponding sub-groove 11a.

It can be seen that this embodiment is equivalent to using negative pressure to discharge the waste liquid from the synthesis hole 21, that is, the negative pressure is applied by the negative pressure unit, and under the communication of the drainage channel 13, a negative pressure is formed in the drainage tank 11. At this time, the lower port of the synthesis hole 21 is equivalent to being in a negative pressure state, so that the waste liquid in the synthesis hole 21 can be sucked into the drainage tank 11 to achieve liquid drainage. The area is also in a normal air pressure state, and no positive pressure will be formed, so that it will not interfere with the normal liquid addition of the liquid addition module 3. In other words, in this embodiment, normal liquid addition operations can still be performed during the liquid discharge process.

Secondly, in this solution, the method of negative pressure drainage is adopted, and the drainage tank 11 is divided into at least two independent sub-slots 11a, and the drainage channel 13 of each sub-slot 11a is provided with a valve member 14, so that after the composite plate 2 and the liquid pumping plate 1 are assembled, a group of synthetic holes 21 corresponds to a sub-slot 11a, so that when in use, the sub-slot 11a can be used as a unit for partitioned drainage. The valve element 14 corresponding to 1a, in this way, a negative pressure environment is formed inside the sub-groove 11a, and liquid is pumped separately, so that it will not interfere with the normal synthesis of the synthesis hole groups corresponding to other sub-grooves 11a.

Since a negative pressure is formed in the sub-tank 11a during liquid discharge, the sealing between the butt joint surfaces of the liquid pumping plate 1 and the synthetic plate 2 is particularly important. Therefore, in this embodiment, as shown in FIG.

Specifically, the first sealing member 15 in this embodiment is an annular sealing ring. In order to install and position the first sealing member 15, as shown in FIG. 1 , the upper surface of the liquid pumping plate 1 in this embodiment is provided with a socket 151 surrounding the drain tank 11. During installation, the first sealing member 15 is inserted into the socket 151, and the composite plate 2 is locked on the upper part of the liquid pumping plate 1 by bolts. At this time, the first sealing member 15 is pressed between the composite plate 2 and the liquid pumping plate 1 to form a seal and prevent air leakage.

In addition, in order to ensure the tightness between the sub-slots 11a and prevent the crosstalk between the sub-slots 11a, in this embodiment, it is also necessary to perform sealing treatment between the partition plate 12 and the composite board 2, specifically:

As shown in FIG. 3 , the discharge device further includes a second seal 16 , which is used to be installed between the adjoining surfaces of the synthetic board 2 and the partition 12 , and extends along the length of the partition 12 .

In this embodiment, the second sealing member 16 is preferably a linear sealing strip. When installed, it is arranged on the top wall of the partition 12 along the length direction of the partition 12, and is pressed against the top wall of the partition 12 by the composite board 2, so as to realize the sealing between the partition 12 and the composite board 2, and ensure the sealing of each sub-slot 11a.

In order to position the second sealing member 16 when the second sealing member 16 is installed, in this embodiment, as shown in FIG. 1 , the top of the partition plate 12 extends upwards from the top surface of the liquid pumping plate 1 to form a convex portion 121. As shown in FIG. 2, the second seal 16 is pressed against the top wall of the slot 22 for sealing.

In order to make the waste liquid in the sub-groove 11a more smoothly guide the drainage channel 13, in this embodiment, as shown in Figure 1, the sub-groove 11a includes a first end 11a1 and a second end 11a2, that is, one end of the sub-groove 11a is used as the first end 11a1, and the opposite end is used as the second end 11a2; The first end 11a1 of the tank 11a is connected, so that the waste liquid in the sub-tank 11a can flow from the second end 11a2 to the first end 11a1 along the inclined tank bottom under its own weight and negative pressure suction, so that it can better flow into the discharge channel 13 of the first end 11a1, and finally be discharged into the negative pressure tank through the discharge channel 13.

Since the first end 11a1 of the sub-groove 11a needs to be led out to install the drain channel 13, and the installation of the drain channel 13 needs to have enough space, but the space between the ends of the same side between two adjacent sub-grooves 11a is limited.

Therefore, in this embodiment, as shown in FIG. 1, in two adjacent sub-grooves 11a, the drainage channels 13 of the two sub-grooves 11a are on the opposite side. From another perspective, it can also be understood that in the two adjacent sub-grooves 11a, the first ends 11a1 of the two sub-grooves 11a are on the opposite side, so that the drainage channels 13 can be misplaced and installed to ensure that the drainage channels 13 can be installed normally.

Example 2

As shown in FIG. 6 , this embodiment further provides a gene synthesizer on the basis of Embodiment 1, which includes a synthesis plate 2, a liquid addition module 3 for adding liquid to the synthesis hole 21 of the synthesis plate 2, and the waste liquid discharge device of the synthesis plate 2 described in Embodiment 1.

The liquid addition module 3 mainly includes several vertically arranged nozzles through which the reagents are sprayed into the corresponding synthesis holes 21. There are specific applications and instructions for this liquid addition module 3 in existing gene synthesizers, so it will not be repeated here.

Since some reagents have certain toxicity during the gene synthesis process, and gene synthesis is generally required to be carried out in a dry environment, so in order to prevent the reagents from volatilizing to the external environment and provide a dry environment for gene synthesis, in this embodiment:

The gene synthesizer also includes a closed synthetic chamber 4, wherein the specific composition of the synthetic chamber 4 is to form a synthetic chamber 4 surrounded by a bottom case 41 and a cover plate 42. Specifically, the bottom case 41 mainly includes a rectangular bottom plate and four rectangular side plates.

7 and 8, the synthesis plate 2 is horizontally arranged in the synthesis chamber 4, and the liquid addition module 3 is arranged on the cover plate 42. In addition, the synthesis chamber 4 is provided with an air inlet 411 for the inlet of the protective gas and an air outlet 412 for exhaust. During specific use, the air inlet 411 is connected to the gas supply device for providing the protective gas, wherein the gas supply device can be a nitrogen tank for providing nitrogen as the protective gas. The air outlet 412 is connected to a post-processing device having an air purification function.

In this way, the protective gas is introduced into the synthesis chamber 4 from the gas supply device through the air inlet 411, and the protective gas brings the saturated steam formed by the volatilization of the reagents in the synthesis chamber 4 into the post-processing device through the gas outlet 412 in the synthesis chamber 4. At this time, it is equivalent to relying on the protection gas to replace the saturated steam in the synthesis chamber 4, thereby ensuring the drying of the synthesis chamber 4 and providing a dry environment for the gene synthesis of the synthesis plate 2, and the replaced saturated steam is passed into the post-processing device for purification. Avoid polluting the environment after its discharge.

In order to enable the shielding gas to better replace the saturated steam in the synthesis chamber 4, the gas inlet 411 and the gas outlet 412 are preferably arranged on opposite side panels.

For the convenience of subsequent maintenance or cleaning in the synthesis chamber 4, in this embodiment, the cover plate 42 is preferably an openable cover on the bottom case 41, so that when the synthesis chamber 4 needs to be maintained or cleaned, the cover plate 42 can be opened to expose the inner space of the synthesis chamber 4.

As shown in FIG. 8 , one of the side plates of the cover plate 42 is preferably hinged on the bottom shell 41, so that the cover plate 42 can be opened or closed in an overturned manner. In addition, in order to make the cover plate 42 able to be firmly covered on the bottom shell 41; several toggle clips 413 are arranged on the bottom shell 41. After the cover plate 42 is closed, the cover plate 42 is compressed on the bottom shell 41 by means of the toggle clips 413, as shown in FIG. 6 .

In order to ensure the sealing performance of the cover plate 42 after being closed, a sealing ring is provided between the abutting surface of the cover plate 42 and the bottom case 41 .

In some high-throughput synthesis plates 2 , since the number of synthesis holes 21 on the synthesis plate 2 is large, and the nozzles of the liquid addition module 3 are less than the number of synthesis holes 21 , the nozzles cannot correspond to the synthesis holes 21 one by one.

Therefore, in this embodiment, in order to realize the liquid addition of the high-throughput synthetic plate 2, as shown in FIG. 7 , the synthetic chamber 4 is provided with a linear module 5, and the synthetic plate 2 and the liquid pumping plate 1 are installed on the linear module 5 and driven by the linear module 5 to move horizontally and linearly; wherein the linear module 5 is preferably a servo screw linear module.

Wherein, a mounting seat 51 is fixedly installed on the sliding seat of the linear module 5, the liquid pumping plate 1 is installed on the mounting seat 51, and the synthetic plate 2 is mounted on the liquid pumping plate 1, so that the linear module 5 drives the mounting seat 51 to move linearly, and then drives the synthetic plate 2 and the liquid pumping plate 1 to move linearly; wherein the valve member 14 on the liquid discharge channel 13 is mounted on the mounting seat 51.

However, the negative pressure unit in the waste liquid discharge device of the synthesis plate 2 is arranged outside the synthesis chamber 4, and the drain channel 13 leads out of the synthesis chamber 4 to connect with the negative pressure unit.

When adding liquid to the high-throughput synthetic plate 2, the synthetic hole 21 corresponding to the nozzle can be adjusted by moving the synthetic plate 2 to add liquid. For example, first move the synthetic plate 2 so that the synthetic holes 21 in odd columns on the synthetic plate 2 are aligned with the nozzles.

After the nozzle has finished adding liquid, sometimes it is necessary to feed cleaning reagent into the nozzle to clean the nozzle. Therefore, in order to accept the waste liquid ejected by the nozzle when cleaning the nozzle, in this embodiment, as shown in FIG.

It will be apparent to those skilled in the art that the invention is not limited to the details of the above-described exemplary embodiments, but that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, no matter from which point of view, all the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, so all changes within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention.

Claims (10)

1. The liquid draining device for the composite board comprises a liquid draining board, wherein the liquid draining board is arranged at the bottom of the composite board and comprises liquid draining grooves with open upper ends, and lower ports of synthetic holes of the composite board are communicated with the liquid draining grooves; the discharging device further comprises a negative pressure unit which is communicated with the liquid discharging channel and used for applying negative pressure to the liquid discharging channel; in the assembled state, the synthetic holes on the synthetic plate are divided into at least two groups, and one combined hole corresponds to one sub-groove.

2. The composite board waste discharge apparatus as claimed in claim 1, wherein the at least two sub-tanks are arranged side by side in sequence along the length direction of the drain tank, and wherein adjacent sub-tanks are partitioned by a partition extending along the width direction of the drain tank.

3. The synthetic panel waste liquid discharge apparatus of claim 2 further comprising a first seal for mounting between the interface of the suction panel and the synthetic panel, the first seal surrounding the periphery of the drain tank; and/or the discharge means further comprises a second seal for mounting between the mating surfaces of the synthetic panel and the baffle and extending along the length of the baffle.

4. A waste liquid discharge apparatus as claimed in claim 3, wherein the top of the partition plate extends upwardly to form a protrusion on the top surface of the liquid suction plate, the bottom wall of the composite plate has a recess for the protrusion to be inserted into corresponding to the partition plate, and the second sealing member is disposed in the recess.

5. The composite board waste discharge device of claim 2, wherein the subslot includes a first end and a second end, the bottom of the subslot having a slope structure gradually sloping downward from the second end toward the first end, and wherein the drain channel communicates with the first end of the subslot.

6. The composite board waste discharge device of claim 2, wherein the drain channel is provided at an end side of the sub-tank to communicate with the sub-tank, and in adjacent two sub-tanks, the drain channels of the two sub-tanks are at opposite sides.

7. A gene synthesizer comprising a synthetic panel and a liquid feeding module for feeding liquid to a synthetic aperture of the synthetic panel, the synthetic panel being connected with a waste liquid discharge device, characterized in that the waste liquid discharge device employs a synthetic panel waste liquid discharge device according to any one of claims 1-6.

8. The gene synthesizer of claim 7 further comprising a closed synthesis chamber, the synthesis plate being horizontally disposed in the synthesis chamber, the synthesis chamber being provided with an air inlet for the passage of a shielding gas and an air outlet for the exhaust of air.

9. The gene synthesizer of claim 8, comprising a bottom shell and a cover plate, wherein the cover plate is openable and covered on the bottom shell, the cover plate and the bottom shell are surrounded to form the synthesis chamber in a covering state, and the liquid adding module is arranged on the cover plate.

10. The gene synthesizer according to claim 8 or 9, wherein a linear module is arranged in the synthesizing chamber, and the synthesizing plate and the liquid extracting plate are both arranged on the linear module and driven by the linear module to move horizontally and linearly; the liquid receiving disc is further installed on the linear module, and the liquid receiving disc and the synthetic plate move synchronously.