CN116474152A - Preparation method and application of Janus membrane based on esterified glucan - Google Patents
Preparation method and application of Janus membrane based on esterified glucan Download PDFInfo
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- CN116474152A CN116474152A CN202310365674.0A CN202310365674A CN116474152A CN 116474152 A CN116474152 A CN 116474152A CN 202310365674 A CN202310365674 A CN 202310365674A CN 116474152 A CN116474152 A CN 116474152A
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- glucan
- esterified
- dextran
- hydrophilic
- hydrophobic
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- 229920001503 Glucan Polymers 0.000 title claims abstract description 42
- 239000012528 membrane Substances 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 36
- 229920002307 Dextran Polymers 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 19
- 230000029663 wound healing Effects 0.000 claims abstract description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 51
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 claims description 51
- 239000000243 solution Substances 0.000 claims description 14
- 229920002558 Curdlan Polymers 0.000 claims description 13
- 239000001879 Curdlan Substances 0.000 claims description 13
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 13
- 229940078035 curdlan Drugs 0.000 claims description 13
- 235000019316 curdlan Nutrition 0.000 claims description 13
- 239000011148 porous material Substances 0.000 claims description 8
- 238000009987 spinning Methods 0.000 claims description 8
- 239000000835 fiber Substances 0.000 claims description 7
- BYEAHWXPCBROCE-UHFFFAOYSA-N 1,1,1,3,3,3-hexafluoropropan-2-ol Chemical compound FC(F)(F)C(O)C(F)(F)F BYEAHWXPCBROCE-UHFFFAOYSA-N 0.000 claims description 6
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 6
- 238000001523 electrospinning Methods 0.000 claims description 5
- 238000010041 electrostatic spinning Methods 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000011068 loading method Methods 0.000 claims description 5
- 230000008439 repair process Effects 0.000 claims description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 229920001543 Laminarin Polymers 0.000 claims description 2
- 239000005717 Laminarin Substances 0.000 claims description 2
- 229920001491 Lentinan Polymers 0.000 claims description 2
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 2
- 244000248825 Peltandra virginica Species 0.000 claims description 2
- 235000001188 Peltandra virginica Nutrition 0.000 claims description 2
- 235000008599 Poria cocos Nutrition 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 claims description 2
- 229940115286 lentinan Drugs 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 16
- 238000005086 pumping Methods 0.000 abstract description 15
- 239000000463 material Substances 0.000 abstract description 13
- 230000008569 process Effects 0.000 abstract description 6
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 229920001282 polysaccharide Polymers 0.000 abstract description 4
- 239000005017 polysaccharide Substances 0.000 abstract description 4
- 238000010276 construction Methods 0.000 abstract description 3
- 230000032050 esterification Effects 0.000 abstract description 3
- 238000005886 esterification reaction Methods 0.000 abstract description 3
- 229920001059 synthetic polymer Polymers 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000010410 layer Substances 0.000 description 45
- 206010052428 Wound Diseases 0.000 description 18
- 208000027418 Wounds and injury Diseases 0.000 description 18
- 239000013060 biological fluid Substances 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 8
- 239000002121 nanofiber Substances 0.000 description 7
- 238000000967 suction filtration Methods 0.000 description 7
- 238000001816 cooling Methods 0.000 description 6
- 238000001035 drying Methods 0.000 description 6
- 230000035699 permeability Effects 0.000 description 6
- 230000001376 precipitating effect Effects 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000700159 Rattus Species 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920002689 polyvinyl acetate Polymers 0.000 description 1
- 239000011118 polyvinyl acetate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a preparation method and application of a Janus membrane based on esterified glucan. The Janus membrane includes a hydrophobic layer esterified dextran and a hydrophilic layer esterified dextran. The invention uses natural polysaccharide as a base material for constructing Janus membrane for the first time, and is different from the current construction using synthetic polymer, the natural glucan has excellent biocompatibility and has the function of accelerating wound healing. The esterified glucan prepared by the invention has no fracture layering phenomenon in the use (water absorption) process, and for most reported unidirectional self-pumping membranes, the membranes are extremely easy to fracture layering in the use process due to different base materials on two sides of the membranes, so that the unidirectional self-pumping capacity of the membranes can be possibly lost. The dextran with different esterification degrees adopted by the invention has very similar structural performance, so that the film has better fitting performance and does not have fracture layering phenomenon in use.
Description
Technical Field
The invention relates to the technical field of natural polysaccharide materials, in particular to a preparation method and application of a Janus membrane based on esterified glucan.
Background
Biological fluid management around wounds is a prerequisite for wound healing, including controlling bleeding and removing excess biological fluid (e.g., wound exudates, sweat, and urine). In recent years, there has been great progress in controlling bleeding from needles, sheet-like particles, and shear-thinning hydrogels, and tough adhesives. However, excess biological fluid is a neglected but ubiquitous problem that hinders the wound healing process. Conventional hydrophilic dressings can typically absorb some of the biological fluid, but due to their inherent hydrophilicity, inevitably leave biological fluid at the interface between the wound and the dressing. The remaining biological fluid constantly moisturizes the wound and complicates the healing process. Therefore, there is an urgent need to develop a new wound dressing to effectively remove excessive biological fluids.
The biological interface plays an important role in the interaction between biological fluids and biological materials. The surface wettability of a wound dressing typically affects the wetting behavior of biological fluids surrounding the wound. As with most conventional dressings, hydrophilic materials are readily wetted by biological fluids, thereby overhydrating the wound. In contrast, as a waterproof outer layer of the dressing, the hydrophobic material may prevent accidental contact of external fluids with the wound, but may not facilitate removal of biological fluids. Recently, some Janus interface materials with asymmetric wettability have shown their unique water droplet transfer capability. Janus material refers to a two-dimensional material that has asymmetric properties on each side, with the properties coming from different components and/or structures. Such materials are widely found in nature and play a vital role in organisms. Due to the unidirectional autonomous liquid transport capability, such materials have attracted considerable attention in the fields of oil-water separation, mist collection, humidity management, etc., and they also provide the idea of preparing a wound dressing-i.e. unidirectional transport behavior. However, these Janus materials are typically synthetic polymers such as polyester fabrics, polyurethane (PU)/polyvinyl acetate composite fiber films and single sided fluorinated cotton fabric films with a wettability gradient, lacking the necessary physiological functions. Therefore, their value in chronic wounds is very limited. Thus, control of surface wettability may provide an opportunity to design a wound dressing with effective biological fluid management capabilities.
The curdlan is a natural polysaccharide polymer which exists in fungal cell walls, and researches show that the curdlan can stimulate the immune system of a human body, enhance the resistance of the human body and effectively promote the repair of wounds. In recent years, the application of curdlan in the fields of biomedicine and medicine is attracting more and more attention, and despite the fact that curdlan has better gel forming property, the curdlan lacks diversified functions and has extremely poor processing property, and the electrospun nanofiber has high porosity, extracellular matrix-like structure, flexible component and extensible form, shows excellent performance in tissue repair, and has wide prospect as a nanoscale construction unit of a multifunctional wound dressing. Recently, we have found that dextran electrospun membranes of different degrees of esterification have different hydrophilic and hydrophobic properties, based on which we constructed an esterified dextran-based electrospun membrane, which was found to have unidirectional self-pumping capability, is a completely new Janus system.
Disclosure of Invention
It is an object of a first aspect of the present invention to provide a Janus film.
The second aspect of the present invention is to provide a method for preparing the Janus membrane.
A third aspect of the present invention is directed to the use of the above Janus film.
A fourth aspect of the invention is directed to a product.
The technical scheme adopted by the invention is as follows:
in a first aspect, the invention provides a Janus membrane, which is characterized by comprising a hydrophobic layer and a hydrophilic layer, wherein the hydrophobic layer is made of esterified glucan of the hydrophobic layer, and the hydrophilic layer is made of esterified glucan of the hydrophilic layer.
In some embodiments of the invention, the hydrophobic layer has an average pore size of 50-100 μm, a porosity of 80-90%, a thickness of 20-40 μm, and a fiber diameter of 300-350nm.
In some embodiments of the invention, the hydrophilic layer has an average pore size of 30-50 μm, a porosity of 70-80%, a thickness of 70-100 μm, and a fiber diameter of 180-250nm.
In a second aspect of the present invention, there is provided a method for preparing a Janus film according to the first aspect of the present invention, comprising the steps of: respectively dissolving hydrophilic esterified glucan and hydrophobic esterified glucan in a solvent to form a hydrophilic solution and a hydrophobic solution, and sequentially carrying out electrostatic spinning superposition film formation.
In some embodiments of the invention, the conditions of electrospinning are: the voltage loading range is +15kV to +30kV, the injection speed is 0.5 mL/min-2 mL/min, the drum speed is 100 rpm-500 rpm, and the spinning interval is 10 cm-25 cm.
In some embodiments of the invention, the concentration of both the hydrophilic solution and the hydrophobic solution is from 3wt% to 8wt%.
In some embodiments of the invention, the volume ratio of the hydrophilic solution to the hydrophobic solution is 1 to 3:1.
in some embodiments of the invention, the method of preparing the hydrophilic esterified glucan and the hydrophobic esterified glucan comprises: mixing dextran, acetic anhydride and acetic acid, and solid-liquid separating. Esterified glucans of different hydrophilic/hydrophobic character can be obtained by controlling the feeding ratio of glucan, acetic anhydride and acetic acid.
In some embodiments of the invention, the ratio of glucan, acetic anhydride, acetic acid in the hydrophilic esterified glucan is M Dextran /g：V Acetic anhydride /ml：V Acetic acid /ml＝1～2：6～10：8～12。
In some embodiments of the invention, the ratio of glucan, acetic anhydride, acetic acid in the hydrophobic esterified glucan is M Dextran /g：V Acetic anhydride /ml：V Acetic acid /ml＝1～2：2～4：2～6。
In some embodiments of the invention, the glucan comprises at least one of yeast glucan, curdlan, lentinan, laminarin, tuckahoe glucan.
In some embodiments of the invention, the conditions of uniform mixing include: 40-70 ℃ and 5-10 h.
In some embodiments of the invention, the solid-liquid separation method comprises: centrifuging, suction filtering, etc.
In some embodiments of the invention, the centrifuging comprises adding 1 to 5 volumes of pure water to the mixed solution for centrifuging.
In some preferred embodiments of the invention, the centrifugation conditions include 5000-10000rpm for 5-10min.
In some embodiments of the invention, the pore size of the suction filtration is 80-120nm.
In some embodiments of the invention, the solvent comprises at least one of absolute ethanol, dichloromethane, tetrahydrofuran, hexafluoroisopropanol, methanol, N-dimethylformamide, acetone.
In a third aspect of the invention there is provided the use of a Janus film according to the first aspect of the invention or a Janus film prepared by a method according to the second aspect of the invention in the manufacture of a product for promoting wound healing and accelerating skin repair.
In a fourth aspect of the invention, there is provided a product comprising a Janus film according to the first aspect of the invention or a Janus film prepared by a method according to the second aspect of the invention.
In some embodiments of the invention, the product is used to promote wound healing, accelerate skin repair.
The beneficial effects of the invention are as follows:
the invention uses natural polysaccharide as a base material for constructing Janus membrane for the first time, and is different from the current construction using synthetic polymer, the natural glucan has excellent biocompatibility and has the function of accelerating wound healing. The esterified glucan prepared by the invention has no fracture layering phenomenon in the use (water absorption) process, and for most reported unidirectional self-pumping membranes, the membranes are extremely easy to fracture layering in the use process due to different base materials on two sides of the membranes, so that the unidirectional self-pumping capacity of the membranes can be possibly lost. The dextran with different esterification degrees adopted by the invention has very similar structural performance, so that the film has better fitting performance and does not have fracture layering phenomenon in use.
Drawings
FIG. 1 is a schematic illustration of the micro-nano structure morphology of the self-pumping film of the present invention;
FIG. 2 is a schematic view of the mechanical properties of the self-pumping film of the present invention;
FIG. 3 is a schematic illustration of droplet transport (unidirectional self-pumping performance) from a pumping membrane according to the present invention;
FIG. 4 is a schematic illustration of the accelerated wound closure of the self-pumping film of the present invention.
Detailed Description
The conception and the technical effects produced by the present invention will be clearly and completely described in conjunction with the embodiments below to fully understand the objects, features and effects of the present invention. It is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and that other embodiments obtained by those skilled in the art without inventive effort are within the scope of the present invention based on the embodiments of the present invention.
Example 1 Janus film based on esterified dextran
The preparation method comprises the following steps:
(1) 5g of curdlan was dispersed in 30mL of acetic anhydride, and the temperature was raised to 60℃with continuous stirring, followed by addition of 40mL of acetic acid. And keeping the reaction system continuously stirred for 5 hours, after the reaction is finished, cooling to room temperature, adding 100mL of pure water, precipitating to separate out white floccules, collecting the floccules, centrifuging for 5 minutes at 8000rpm to enable the floccules to be gathered together, and drying after suction filtration by using a Buchner funnel with the inner diameter of 100mm to obtain the esterified glucan with the hydrophilic layer.
(2) 5g of curdlan was dispersed in 20mL of acetic anhydride, and the temperature was raised to 60℃with continuous stirring, followed by addition of 10mL of acetic acid. And keeping the reaction system continuously stirred for 5 hours, after the reaction is finished, cooling to room temperature, adding 100mL of pure water, precipitating to separate out white floccules, collecting the floccules, centrifuging for 5 minutes at 8000rpm to enable the floccules to be gathered together, and drying after suction filtration by using a Buchner funnel to obtain the esterified glucan with the hydrophobic layer.
(3) 10mL of hexafluoroisopropanol solution of dextran esterified to hydrophilic layer and 5mL of hexafluoroisopropanol solution of dextran esterified to hydrophobic layer were prepared to a concentration of 5wt% respectively. Manufacturing a double-layer film in a high-voltage electrostatic spinning machine, wherein the spinning conditions are controlled as follows: and (3) carrying out voltage loading of +25kV, injection speed of 1mL/min, drum speed of 300rpm and spinning interval of 15cm (the electrospinning conditions of the hydrophilic layer and the hydrophobic layer are the same), and finally obtaining the Janus membrane based on esterified glucan.
Example 2 Janus film based on esterified dextran
The preparation method comprises the following steps:
(1) 5g of curdlan was dispersed in 40mL of acetic anhydride, and the temperature was raised to 60℃with continuous stirring, followed by addition of 40mL of acetic acid. And keeping the reaction system continuously stirred for 5 hours, after the reaction is finished, cooling to room temperature, adding 100mL of pure water, precipitating to separate out white floccules, collecting the floccules, centrifuging for 5 minutes at 8000rpm to enable the floccules to be gathered together, and drying after suction filtration by using a Buchner funnel to obtain the hydrophilic layer esterified glucan.
(2) 5g of curdlan was dispersed in 20mL of acetic anhydride, and the temperature was raised to 60℃with continuous stirring, followed by addition of 15mL of acetic acid. And keeping the reaction system continuously stirred for 5 hours, after the reaction is finished, cooling to room temperature, adding 100mL of pure water, precipitating to separate out white floccules, collecting the floccules, centrifuging for 5 minutes at 8000rpm to enable the floccules to be gathered together, and drying after suction filtration by using a Buchner funnel to obtain the esterified glucan with the hydrophobic layer.
(3) 10mL of the hydrophilic layer esterified dextran and 5mL of the hydrophobic layer esterified dextran were each dissolved in hexafluoroisopropanol to give a concentration of 6wt%. Manufacturing a double-layer film in a high-voltage electrostatic spinning machine, wherein the spinning conditions are controlled as follows: and (3) carrying out voltage loading of +25kV, injection speed of 1mL/min, drum speed of 300rpm and spinning interval of 15cm (the electrospinning conditions of the hydrophilic layer and the hydrophobic layer are the same), and finally obtaining the Janus membrane based on esterified glucan.
Example 3 Janus film based on esterified dextran
The preparation method comprises the following steps:
(1) 5g of curdlan was dispersed in 50mL of acetic anhydride, and the temperature was raised to 60℃with continuous stirring, followed by addition of 30mL of acetic acid. And keeping the reaction system continuously stirred for 5 hours, after the reaction is finished, cooling to room temperature, adding 100mL of pure water, precipitating to separate out white floccules, collecting the floccules, centrifuging for 5 minutes at 8000rpm to enable the floccules to be gathered together, and drying after suction filtration by using a Buchner funnel to obtain the hydrophilic layer esterified glucan.
(2) 5g of curdlan was dispersed in 30mL of acetic anhydride, and the temperature was raised to 60℃with continuous stirring, followed by addition of 10mL of acetic acid. And keeping the reaction system continuously stirred for 5 hours, after the reaction is finished, cooling to room temperature, adding 100mL of pure water, precipitating to separate out white floccules, collecting the floccules, centrifuging for 5 minutes at 8000rpm to enable the floccules to be gathered together, and drying after suction filtration by using a Buchner funnel to obtain the esterified glucan with the hydrophobic layer.
(3) 10mL of the hydrophilic layer esterified dextran and 5mL of the hydrophobic layer esterified dextran were each dissolved in hexafluoroisopropanol to give a concentration of 5wt%. Manufacturing a double-layer film in a high-voltage electrostatic spinning machine, wherein the spinning conditions are controlled as follows: and (3) carrying out voltage loading of +25kV, injection speed of 1mL/min, drum speed of 300rpm and spinning interval of 15cm (the electrospinning conditions of the hydrophilic layer and the hydrophobic layer are the same), and finally obtaining the Janus membrane based on esterified glucan.
Effect example 1
The surface morphology of the two-layer electrospun film obtained in the foregoing example 1 was examined.
Esterified dextran Janus was cut into squares and metal blasted to observe its surface morphology and pore size using a scanning electron microscope.
Experimental results: FIG. 1 shows a bilayer Janus membrane having a thickness of 109 μm, a hydrophilic layer fiber diameter of 204nm, an average pore size of 38 μm, a porosity of 74%, and a thickness of 75 μm; the hydrophobic layer had a fiber diameter of 343nm, an average pore diameter of 78 μm, a porosity of 84% and a thickness of 34. Mu.m.
Effect example 2
The air permeability of the two-layer electrospun film obtained in the foregoing example 1 was measured.
The wound dressing should be breathable and moisture permeable to maintain gas exchange at the wound site. The air permeability of the nanofiber membrane was measured using an automatic air permeability meter. The internal and external pressure difference of the nanofiber membrane is controlled to be 100Pa, and the test area is 20cm 2 . The test was performed randomly at 10 positions of the nanofiber membrane.
Experimental results: the air permeability of the double-layer electrospun film was 7.25+1.25mm/s。
Effect example 3
The water vapor permeability test was performed on the two-layer electrospun film obtained in the foregoing example 1.
The sample was covered with a cylindrical cup containing distilled water and placed in a constant temperature and humidity box. The ambient parameters temperature, relative humidity and airflow were 38 ℃, 2% and 0.5m/s, respectively. Each sample was tested 3 times and the average was taken as the water vapor transmission rate of the nanofiber membrane.
Experimental results: the air permeability of the double-layer electrospun film is 2167+205g/m 2 /day。
Effect example 4
The mechanical properties of the two-layer electrospun film obtained in the foregoing example 1 were tested.
And testing the mechanical properties of the nanofiber membrane by using an electronic tensile tester. All films were cut into 5 pieces 50X 10mm 2 And accurately measure the thickness of each sample. Each sample was then tested 5 times at a crosshead speed of 10mm/min and the average of tensile strength and elongation at break was recorded.
Experimental results: the tensile strength is 240-253kPa, and the elongation at break is about 12%.
Effect example 5
The two-layer electrospun film obtained in the foregoing example 1 was subjected to a droplet diffusivity test to evaluate its self-pumping performance.
The diffusion rate of the droplets was tested using 50 μl deionized water with blue ink to study the water transport process in the nanofiber membrane. The membrane was placed horizontally on the top and bottom sides during the measurement. The diffusion diameter of the droplet in the monolayer fibrous film and self-pumping dressing was recorded every 10 seconds.
Experimental results: fig. 3 shows that the self-pumping membrane can transfer droplets from the hydrophobic layer to the hydrophilic layer by hydrophilic/hydrophobic properties, preventing liquid from exuding.
Effect example 6
The ability to accelerate skin repair from the pump membrane was assessed by full-thickness skin defect experiments.
Rats were divided into 4 groups of 3, two holes of 8mm in size and diameter were made in the backs of the rats, and then a single-layer film (hydrophilic layer and hydrophobic layer) and a double-layer film (self-pumping layer) of example 1 were placed therein, respectively, and a blank control group was set, and wound defect recovery was observed.
Experimental results: the results of fig. 4 show that the application of self-pumping membranes accelerates wound repair.
The present invention has been described in detail in the above embodiments, but the present invention is not limited to the above examples, and various changes can be made within the knowledge of those skilled in the art without departing from the spirit of the present invention. Furthermore, embodiments of the invention and features of the embodiments may be combined with each other without conflict.
Claims (10)
1. The Janus membrane is characterized by comprising a hydrophobic layer and a hydrophilic layer, wherein the hydrophobic layer is made of esterified glucan, and the hydrophilic layer is made of esterified glucan.
2. The Janus membrane of claim 1, wherein the hydrophobic layer has an average pore size of 50-100 μm, a porosity of 80-90%, a thickness of 20-40 μm and a fiber diameter of 300-350nm; preferably, the hydrophilic layer has an average pore size of 30-50 μm, a porosity of 70-80%, a thickness of 70-100 μm and a fiber diameter of 180-250nm.
3. The method for preparing the Janus film according to any one of claims 1 to 2, comprising the steps of: respectively dissolving hydrophilic esterified glucan and hydrophobic esterified glucan in a solvent to form a hydrophilic solution and a hydrophobic solution, and sequentially carrying out electrostatic spinning superposition film formation.
4. A method according to claim 3, wherein the conditions of electrospinning are: the voltage loading range is +15kV to +30kV, the injection speed is 0.5 mL/min-2 mL/min, the drum speed is 100 rpm-500 rpm, and the spinning interval is 10 cm-25 cm.
5. The method of claim 3, wherein the hydrophilic solution and the hydrophobic solution are each at a concentration of 3wt% to 8wt%; preferably, the volume ratio of the hydrophilic solution to the hydrophobic solution is 1-3: 1.
6. a method according to claim 3, wherein the preparation of hydrophilic esterified dextran and hydrophobic esterified dextran comprises: uniformly mixing glucan, acetic anhydride and acetic acid, and then carrying out solid-liquid separation;
preferably, the ratio of glucan, acetic anhydride and acetic acid in the hydrophilic esterified glucan is M Dextran /g：V Acetic anhydride /ml：
V Acetic acid /ml＝1～2：6～10：8～12；
Preferably, the dextran, acetic anhydride and acetic acid ratio in the hydrophobic esterified dextran is M Dextran /g：V Acetic anhydride /ml：V Acetic acid /ml＝1～2：2～4：2～6。
7. The method of claim 6, wherein the glucan comprises at least one of yeast glucan, curdlan, lentinan, laminarin, tuckahoe glucan.
8. A method according to claim 3, wherein the solvent comprises at least one of absolute ethanol, dichloromethane, tetrahydrofuran, hexafluoroisopropanol, N-dimethylformamide, acetone.
9. Use of a Janus film according to any one of claims 1 to 2 or a Janus film prepared by a method according to any one of claims 3 to 8 for the preparation of a product for promoting wound healing and accelerating skin repair.
10. A product comprising a Janus film according to any one of claims 1 to 2 or a Janus film prepared by the method of any one of claims 3 to 8.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6753454B1 (en) * | 1999-10-08 | 2004-06-22 | The University Of Akron | Electrospun fibers and an apparatus therefor |
| CN109778430A (en) * | 2019-01-28 | 2019-05-21 | 吉林农业大学 | A preparation method for preparing Janus structure nanofibers by uniaxial electrospinning |
| CN109908392A (en) * | 2019-03-27 | 2019-06-21 | 广州创赛生物医用材料有限公司 | Novel asymmetric wettability electrostatic spinning duplicature of one kind and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6753454B1 (en) * | 1999-10-08 | 2004-06-22 | The University Of Akron | Electrospun fibers and an apparatus therefor |
| CN109778430A (en) * | 2019-01-28 | 2019-05-21 | 吉林农业大学 | A preparation method for preparing Janus structure nanofibers by uniaxial electrospinning |
| CN109908392A (en) * | 2019-03-27 | 2019-06-21 | 广州创赛生物医用材料有限公司 | Novel asymmetric wettability electrostatic spinning duplicature of one kind and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 吴朝希: "β-葡聚糖的酯化、自组织行为及其作为皮肤敷料的研究", 《中国博士学位论文全文数据库 医药卫生科技辑》, 15 February 2016 (2016-02-15), pages 64 - 67 * |
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