CN116421651A - A kind of external bactericide and acaricide for animals and preparation method thereof - Google Patents

Abstract

The present invention relates to the technical field of bactericides, in particular to an external bactericidal and acaricidal agent for animals, which comprises the following components: Folium Folium Extract, Coptis Rhizoma Extract, Wintergreen Essential Oil, Peppermint Extract, Dandelion Extract, Honeysuckle Extract, quaternary ammonium salt, cationic guar gum, deionized water; in addition, the use of bactericidal and mite-removing agents does not cause any harm to pet fur, has many types of sterilization, has no irritating smell, and is friendly to the human body.

Description

A kind of external bactericide and acaricide for animals and preparation method thereof

technical field

The invention relates to the technical field of fungicides, in particular to an external fungicide and acaricide for animals and a preparation method thereof.

Background technique

With the improvement of living standards, pets such as cats, dogs, and rabbits have become popular among young people. Bacteria such as Salmonella, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, etc. are easily carried on the hair or skin of pets. Due to pets' low immunity, skin damage and other reasons, skin bacteria can easily cause skin infections. Although this infection is not fatal, it can cause sepsis and even death. In addition, since animal skin pathogenic bacteria are also the main source of human fungal ringworm, pet owners may be infected, which makes the pathogenic bacteria spread among the crowd, and large-scale outbreaks are prone to occur.

At present, pet owners often bathe their pets with their own shampoo or shower gel. Although they can have a certain cleaning effect, the bactericidal effect is limited. Or use some fungicides containing irritating odors to mix with water to bathe the pets. Although it can have a good bactericidal effect, it is harmful to the hair or skin of animals, and it is also unfriendly to the human body.

Contents of the invention

In order to solve the technical problems mentioned in the above-mentioned background technology or at least partially solve the above-mentioned technical problems, the object of the present invention is to provide an external bactericidal and acaricidal agent for animals and a preparation method thereof. There is no harm to the fur, there are many types of sterilization, no irritating smell, and it is friendly to the human body.

To achieve the above object, the present invention is achieved through the following schemes;

An external bactericidal and acaricidal agent for animals, comprising the following components: Folium Folium Extract, Coptidis Rhizoma Extract, Wintergreen Essential Oil, Peppermint Extract, Dandelion Extract, Honeysuckle Extract, Quaternary Ammonium Salt, Cationic Guar Gum ,Deionized water.

Folium Folium is bitter in taste and cold in nature, and its extract has broad-spectrum antibacterial effect. Studies have shown that it is effective against Gram-negative bacteria such as Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus A, Gram-positive bacilli, and typhoid bacilli. It has inhibitory effect; in addition, it can also improve the phagocytosis of white blood cells in animals, inhibit the skin reaction caused by xylene, reduce the permeability of capillaries, and play a role in improving the immune function of the body.

Coptidis is bitter and cold in nature. Its extract contains berberine, coptisine, flavonoids and other ingredients. Streptococcus, etc. have obvious inhibitory effect, and at the same time, it also has certain inhibitory effect on Escherichia coli, typhoid bacillus, etc., and can effectively prevent and control the inflammatory response caused by the above-mentioned bacterial infection.

The main active ingredient of wintergreen essential oil is methyl salicylate, which has a fresh mint smell. Mildew, Trichoderma, Candida albicans, etc. have a good inhibitory effect, and have significant acaricidal activity against mites.

Peppermint is pungent and cool in nature, and belongs to the lung and liver meridians. Its extract has the functions of cooling and relieving itching, refreshing the mind, evacuating wind and cold, anti-inflammatory and antibacterial, invigorating the stomach and eliminating food, etc. It can be used for skin itching and indigestion.

Dandelion is bitter and sweet in nature, and the saponins in its extract have a strong inhibitory effect on Staphylococcus aureus, Staphylococcus albus, Hemolytic Streptococcus, Escherichia coli, Diococcus catarrhalis, Bacillus typhi, etc., and can significantly improve peripheral blood circulation. Increase the transformation rate of blood lymphocytes and blasts, thereby enhancing the immune function of the body.

Honeysuckle tastes sweet and slightly bitter, and cold in nature. Its extract has antibacterial effects on typhoid bacillus, paratyphoid bacillus, E. All kinds of bacteria such as inflammatory coccus have inhibitory effect.

Quaternary ammonium salts are positively charged in water, and the positively charged cations adsorb the negatively charged microbial surfaces to form microclusters, which gather on the cell wall to produce room resistance effect, resulting in the inhibition of microbial growth and death; at the same time, its hydrophobic base and microbial The effect of the hydrophilic group changes the permeability of the membrane, and then ruptures the microbial cell membrane, causing the apoptosis of the bacteria, and achieves the best bactericidal effect, mainly for the bactericidal effect on negatively charged bacteria and viruses.

Cationic guar gum has the functions of foam stabilization and antistatic effect, which can improve the wet combing and dry combing properties of hair, increase the retention of bactericidal ingredients on the hair, make the hair shiny and healthy, and keep it easy to comb, bright and free of static electricity feature.

Preferably, thickener, frankincense essential oil are also included.

Frankincense essential oil has a certain antibacterial effect, and when used in combination with the above-mentioned plant extracts and quaternary ammonium salts, it can improve the bactericidal and antibacterial effect of the fungicide as a whole. In addition, it also has a deodorizing effect, which can remove the peculiar smell on the pet's body surface.

Preferably, the quaternary ammonium salt is at least one of peroxybenzyl alkylmethyl quaternary ammonium salt and methyl sulfated alkyl methyl quaternary ammonium salt.

Peroxide benzyl alkyl methyl quaternary ammonium salt and methyl sulfate alkyl methyl quaternary ammonium salt are three-chain quaternary ammonium salts. Their unique three-chain space structure makes them not only have plane shear force, but also have double The three-dimensional torque that chain quaternary ammonium salts and single chain quaternary ammonium salts do not have. The three-chain quaternary ammonium salt not only increases the three-dimensional torque, but also makes it use functional groups to inactivate the enzymes on the microbial cell membrane while changing the permeability of the microbial cell membrane, thereby completely killing the microorganism.

Preferably, the following components are included in parts by weight: 0.2-1.5 parts of Folium Isatidis extract, 0.2-1.5 parts of Coptidis Rhizoma extract, 0.2-0.5 parts of wintergreen essential oil, 0.2-1.5 parts of peppermint extract, dandelion extract 0.2-1.5 parts, 0.2-1.5 parts of honeysuckle extract, 1-6 parts of quaternary ammonium salt, 0.2-0.8 parts of cationic guar gum, 70-85 parts of deionized water.

Preferably, the following components are also included in parts by weight: 0.5-2 parts of a thickener, and 0.2-0.8 parts of frankincense essential oil.

A preparation method of an external bactericidal and acaricide for animals, comprising the following steps: first heating deionized water to 65-75°C, adding quaternary ammonium salts and cationic guar gum in parts by weight, stirring evenly, and then cooling down to 40°C -45°C, then add the remaining components and stir evenly.

The application has the following beneficial effects:

(1) It has a good sterilization effect on Staphylococcus aureus, Escherichia coli, typhoid bacillus, Mycobacterium tuberculosis, streptococcus, Brucella and other bacteria commonly found on pets, and has no pungent smell.

(2) Mild in nature, non-irritating to animal fur and human skin, good sterilization and disinfection effect, and at the same time, it has a care effect on pet fur. After washing, pet hair will not be knotted, and it is shiny, soft and easy to wear Grooming, ideal for daily care of furry pets.

Detailed ways

The present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited to the range expressed in the examples.

Example 1

An external bactericidal and acaricide agent for animals, comprising the following components in parts by weight: 0.2 parts of Daqing leaf extract, 0.2 parts of Coptis chinensis extract, 0.2 parts of wintergreen essential oil, 1.2 parts of peppermint extract, and dandelion extract 0.8 parts, 1.0 parts of honeysuckle extract, 2 parts of quaternary ammonium salt, 0.4 parts of cationic guar gum, 70 parts of deionized water, 0.5 parts of thickener, 0.2 parts of frankincense essential oil.

Coptis chinensis extract is prepared by the following method: Weigh 100 g of Coptis rhizome root powder and add it to 500 g of water, soak for 1 hour at 75° C. When the coarse powder mixture is extracted for the first time, the extraction time is 5 hours, and then the remaining liquid is added to the remaining liquid after the first extraction, and the second extraction is performed after 2 times the amount of water, and the extraction time is 5 hours , and finally add water of 2 times the amount of the remaining liquid to the remaining liquid after the second extraction, and then carry out the third extraction, the extraction time is 2 hours, and three extractions are obtained; the three extractions are filtered to obtain the first-grade Filtrate; when the primary filtrate is concentrated to 80°C, the relative density is 1.2; gradually add ethanol with a concentration of 95% to the primary concentrate to make the alcohol content 40%, at a temperature of 80°C Under the conditions of heat preservation for 30 min, then stand still for 8 hours to obtain the alcoholized solution; discard the upper layer of oil from the alcoholized solution, and filter the lower layer of liquid medicine to obtain the secondary filtrate; after the secondary filtrate is recovered with ethanol, it is concentrated to 80 ° C. The secondary concentrate with a density of 1.3 is the extract of Coptidis rhizome.

The dandelion extract is prepared by the following method: weigh 100g of dandelion, add it to 800g of water, soak it for 1 hour, decoct it for 2 hours, and filter it to obtain the primary filtrate; Grade filtrate; add 300g water to the dregs and then re-decoct for the third time, decoct for 30 min, filter to get the third-grade filtrate, combine the second and third-grade filtrates, first concentrate at low temperature and then mix with the primary filtrate, and finally concentrate to quality The extract with a concentration of 0.5g/mL is ready for use, that is, every 1mL of extract is equivalent to 0.5g of dandelion.

The honeysuckle extract is prepared by the following method: Weigh 100g of dandelion and add it to 800g of water, soak it for 1 hour, decoct it for 2 hours, and filter to obtain the primary filtrate; Grade filtrate; add 300g water to the dregs and then re-decoct for the third time, decoct for 30 min, filter to get the third-grade filtrate, combine the second and third-grade filtrates, first concentrate at low temperature and then mix with the primary filtrate, and finally concentrate to quality The extract with a concentration of 0.8g/mL is ready for use, that is, every 1mL of the extract is equivalent to 0.8g of honeysuckle.

The thickener is composed of carbomer, glycerin and butanediol mixed in a mass ratio of 1:8:6.

The quaternary ammonium salt is benzylalkylmethyl peroxide quaternary ammonium salt.

A preparation method of an external-use bactericidal and acaricide for animals, comprising the following steps: weighing each component by parts by weight, first heating deionized water to 65°C, adding quaternary ammonium salt and cationic guar gum, stirring After uniformity, cool down to 45°C, then add the remaining components, stir evenly, and filter.

Example 2

An external bactericidal and acaricide agent for animals, comprising the following components in parts by weight: 1.5 parts of Daqing leaf extract, 0.5 parts of Coptis chinensis extract, 0.5 parts of wintergreen essential oil, 1 part of peppermint extract, and 1.2 parts of dandelion , 0.2 part of honeysuckle extract, 1 part of quaternary ammonium salt, 0. part of cationic guar gum, 7 parts of deionized water, part of thickener, 0.4 part of frankincense essential oil.

The preparation methods of Coptidis Rhizoma Extract, Dandelion Extract and Honeysuckle Extract are all the same as in Example 1.

The thickener is composed of carbomer, glycerin and butanediol mixed in a mass ratio of 1:8:6.

The quaternary ammonium salt is benzylalkylmethyl peroxide quaternary ammonium salt.

A preparation method of an external-use bactericidal and acaricide for animals, comprising the following steps: weighing each component by parts by weight, first heating deionized water to 70°C, adding quaternary ammonium salt and cationic guar gum, stirring After uniformity, cool down to 45°C, then add the remaining components, stir evenly, and filter.

Example 3

An external bactericidal and acaricide agent for animals, comprising the following components in parts by weight: 0.7 parts of Daqing leaf extract, 0.6 parts of Coptis chinensis extract, 0.4 parts of wintergreen essential oil, 0.23 parts of peppermint extract, and dandelion extract 1.5 parts, 0.5 parts of honeysuckle extract, 6 parts of quaternary ammonium salt, 0.6 parts of cationic guar gum, 80 parts of deionized water, 1.5 parts of thickener, 0.6 parts of frankincense essential oil.

The preparation methods of Coptidis Rhizoma Extract, Dandelion Extract and Honeysuckle Extract are all the same as in Example 1.

The thickener is composed of carbomer, glycerin and butanediol mixed in a mass ratio of 1:8:6.

The quaternary ammonium salt is methyl sulfated alkyl methyl quaternary ammonium salt.

A preparation method of an external-use bactericidal and acaricide for animals, comprising the following steps: weighing each component by parts by weight, first heating deionized water to 75°C, adding quaternary ammonium salt and cationic guar gum, and stirring evenly After cooling down to 40°C, add the remaining components and stir evenly, then filter.

Example 4

An external bactericidal and acaricide agent for animals, comprising the following components in parts by weight: 1.1 parts of Daqing leaf extract, 1.3 parts of Coptis chinensis extract, 0.3 parts of wintergreen essential oil, 0.7 parts of peppermint extract, and dandelion extract 0.2 parts, 1.5 parts of honeysuckle extract, 4 parts of quaternary ammonium salt, 0.8 parts of cationic guar gum, 85 parts of deionized water, 2 parts of thickener, 0.7 parts of frankincense essential oil.

The preparation methods of Coptidis Rhizoma Extract, Dandelion Extract and Honeysuckle Extract are all the same as in Example 1.

The thickener is composed of carbomer, glycerin and butanediol mixed in a mass ratio of 1:8:6.

The quaternary ammonium salt is methyl sulfated alkyl methyl quaternary ammonium salt.

A preparation method of an external-use bactericidal and acaricide for animals, comprising the following steps: weighing each component by parts by weight, first heating deionized water to 75°C, adding quaternary ammonium salt and cationic guar gum, stirring After uniformity, cool down to 40°C, then add the remaining components, stir evenly, and filter.

Example 5

An external bactericidal and acaricide agent for animals, comprising the following components in parts by weight: 0.5 parts of Daqing leaf extract, 0.5 parts of Coptis chinensis extract, 0.3 parts of wintergreen essential oil, 0.2 parts of peppermint extract, and dandelion extract 0.6 parts, 0.8 parts of honeysuckle extract, 5 parts of quaternary ammonium salt, 0.5 parts of cationic guar gum, 80 parts of deionized water, 0.8 parts of thickener, and 0.5 parts of frankincense essential oil.

The preparation methods of Coptidis Rhizoma Extract, Dandelion Extract and Honeysuckle Extract are all the same as in Example 1.

The thickener is composed of carbomer, glycerin and butanediol mixed in a mass ratio of 1:8:6.

The quaternary ammonium salt is composed of peroxidized benzyl alkyl methyl quaternary ammonium salt and sulfuric acid methylated alkyl methyl quaternary ammonium salt in a weight ratio of 1:1.

A preparation method of an external-use bactericidal and acaricide for animals, comprising the following steps: weighing each component by parts by weight, first heating deionized water to 70°C, adding quaternary ammonium salt and cationic guar gum, stirring After uniformity, cool down to 40°C, then add the remaining components, stir evenly, and filter.

Example 6

An external bactericidal and acaricide agent for animals, comprising the following components in parts by weight: 1.2 parts of Daqing leaf extract, 0.8 parts of Coptis chinensis extract, 0.4 parts of wintergreen essential oil, 1.5 parts of peppermint extract, and dandelion extract 1 part, 1.2 parts of honeysuckle extract, 3 parts of quaternary ammonium salt, 0.7 part of cationic guar gum, 80 parts of deionized water, 1.2 parts of thickener, 0.8 part of frankincense essential oil.

The preparation methods of Coptidis Rhizoma Extract, Dandelion Extract and Honeysuckle Extract are all the same as in Example 1.

The thickener is composed of carbomer, glycerin and butanediol mixed in a mass ratio of 1:8:6.

The quaternary ammonium salt is composed of peroxidized benzyl alkyl methyl quaternary ammonium salt and sulfuric acid methylated alkyl methyl quaternary ammonium salt in a weight ratio of 1:1.

A preparation method of an external-use bactericidal and acaricide for animals, comprising the following steps: weighing each component by parts by weight, first heating deionized water to 70°C, adding quaternary ammonium salt and cationic guar gum, stirring After uniformity, cool down to 40°C, then add the remaining components, stir evenly, and filter.

Bacteriostatic effect test

Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella, Candida albicans, and Aspergillus niger were used as test plates for testing strains, and each strain was repeated 3 times. In the test plate, use a sterilized knife to cut a 1cm*1cm square on the test plate, use tweezers to pick out the middle medium to form a square, and add 20 μL of the concentration of 10 μg/mL to the square to prepare it according to Example 1-6 of the present invention Bacteria were cultured in a 37°C incubator for 24 hours. The blank control group used deionized water, and then the size of the inhibition zone (mm ² ) was tested. The results of the inhibition zone test are shown in Table 1.

Table 1

bacteria Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 blank group Staphylococcus aureus 27 30 48 44 38 44 5 Escherichia coli 25 27 44 41 36 41 5 Pseudomonas aeruginosa 26 28 46 43 37 42 5 salmonella twenty four 26 43 41 35 42 5 Candida albicans 25 27 45 40 38 42 5 Aspergillus niger 20 twenty two 36 32 30 33 5

As can be seen from Table 1, the bactericides prepared in Examples 1-6 have significant inhibitory effects on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella, Candida albicans, Aspergillus niger, and Example 3 obtained The bactericidal and bacteriostatic effect of the bactericide is the best, and the bactericidal and bacteriostatic effect of the bactericides prepared in Example 4 and Example 6 is next, and the difference is not large.

Take 10-15ml of the fungicide prepared in Example 3 and dissolve it in 15L of warm water (40-45°C), and use it to bathe the pet dog, rub the pet for 3-5min along the direction of hair growth, and then rinse it with warm water , and then dry the hair with a dry towel; then conduct a test and find that the common Escherichia coli, Staphylococcus aureus, Salmonella, Mycobacterium tuberculosis, Salmonella typhi, Streptococcus, Brucella, etc., have a killing rate of 97% At the same time, it was found that there was no harm to the pet dog's fur, even if the pet dog licked the bath water, no obvious adverse reactions were seen; after sterilization, it can remove the pet dog's fur odor and soothe the fur.

The above descriptions are only preferred implementations of the present invention, and the scope of protection of the present invention is not limited to the above examples, and all technical solutions that fall under the idea of the present invention belong to the scope of protection of the present invention. It should be pointed out that for those skilled in the art, some improvements and modifications without departing from the principles of the present invention should also be regarded as the protection scope of the present invention.

Claims (6)

1. An external sterilizing and mite-killing agent for animals is characterized by comprising the following components: folium Isatidis extract, coptidis rhizoma extract, herba Ilicis Pubescentis essential oil, herba Menthae extract, herba Taraxaci extract, flos Lonicerae extract, quaternary ammonium salt, cationic guar gum, and deionized water.

2. The external bactericidal mite-killing agent for animals according to claim 1, further comprising a thickener, olibanum essential oil.

3. The external bactericidal mite-killing agent for animals according to claim 1, wherein said quaternary ammonium salt is at least one of benzyl alkyl methyl quaternary ammonium peroxide, methyl alkyl methyl quaternary ammonium sulfate.

4. The external sterilizing and mite-killing agent for animals according to any one of claims 1 to 4, which is characterized by comprising the following components in parts by weight: 0.2-1.5 parts of dyers woad leaf extract, 0.2-1.5 parts of coptis root extract, 0.2-0.5 part of wintergreen essential oil, 0.2-1.5 parts of peppermint extract, 0.2-1.5 parts of dandelion extract, 0.2-1.5 parts of honeysuckle extract, 1-6 parts of quaternary ammonium salt, 0.2-0.8 part of cationic guar gum and 70-85 parts of deionized water.

5. The external sterilizing and mite-killing agent for animals according to claim 4, further comprising the following components in parts by weight: 0.5-2 parts of thickener and 0.2-0.8 part of olibanum essential oil.

6. A method of preparing a topical bactericidal mite-killing agent for animals according to any of claims 1-5, comprising the steps of: heating deionized water to 65-75 ℃, adding quaternary ammonium salt and cationic guar gum according to parts by weight, uniformly stirring, cooling to 40-45 ℃, adding the rest components, and uniformly stirring.