CN116399933A - Agarose gel electrophoresis device - Google Patents

Abstract

The invention provides an agarose gel electrophoresis device. The agarose gel electrophoresis apparatus includes: the base is provided with a containing groove; the electrophoresis boxes are used for accommodating agarose gel, the electrophoresis boxes are multiple, and the electrophoresis boxes are arranged in the accommodating groove at intervals; the electrode components are multiple, the multiple electrode components are arranged in one-to-one correspondence with the multiple electrophoresis boxes, each electrode component comprises a positive electrode piece and a negative electrode piece, and the positive electrode piece and the negative electrode piece are respectively positioned on two sides of the electrophoresis box. The invention solves the problem that the sample in the prior art is easy to generate cross infection when subjected to agarose gel electrophoresis.

Description

Agarose gel electrophoresis device

Technical Field

The invention relates to the technical field of biological detection equipment, in particular to an agarose gel electrophoresis device.

Background

Agarose gel electrophoresis (agarose gel electrophoresis) is mainly an electrophoresis method using agarose gel as a support, and by molecular sieve action, nucleic acid fragments are separated by differences in molecular weight or molecular shape and electrophoresis movement speed, and is a method commonly used in genetic experimental operations.

Agarose gel recovery refers to: after agarose gel electrophoresis of a gene (DNA) sample, a gel block containing a target gene (DNA) fragment is cut under an ultraviolet lamp, and after dissolution under a specific reagent, the target gene (DNA) is recovered and purified by a certain method.

Agarose gel electrophoresis in the prior art is to put into electrophoresis buffer solution and gel with a plurality of holes in an electrophoresis tank, then to carry out electrophoresis after adding samples to be tested of the same kind or different kinds in the holes, when adding samples, because the samples are easy to diffuse in liquid, cross contamination can be caused to the samples of other holes, subsequent experiment failure (such as subsequent experiments requiring sequencing, PCR and the like) can be directly caused, and immeasurable loss can be caused to precious samples.

From the above, the conventional technique has a problem that cross infection is likely to occur when a sample is subjected to agarose gel electrophoresis.

Disclosure of Invention

The invention mainly aims to provide an agarose gel electrophoresis device which is used for solving the problem that cross infection is easy to occur when samples are subjected to agarose gel electrophoresis in the prior art.

In order to achieve the above object, the present invention provides an agarose gel electrophoresis apparatus comprising: the base is provided with a containing groove; the electrophoresis boxes are used for accommodating agarose gel, the electrophoresis boxes are multiple, and the electrophoresis boxes are arranged in the accommodating groove at intervals; the electrode components are multiple, the multiple electrode components are arranged in one-to-one correspondence with the multiple electrophoresis boxes, each electrode component comprises a positive electrode piece and a negative electrode piece, and the positive electrode piece and the negative electrode piece are respectively positioned on two sides of the electrophoresis box.

Further, the middle part of the bottom of the accommodating groove is provided with a boss, the boss extends along the arrangement direction of the plurality of electrophoresis boxes, and the bottom of the electrophoresis box is provided with an accommodating concave part matched with the boss.

Further, the agarose gel electrophoresis device further comprises a plurality of partition boards, the partition boards are vertically arranged on the boss and are arranged at intervals along the arrangement direction of the electrophoresis boxes, and two adjacent partition boards form a clamping groove for limiting the electrophoresis boxes.

Further, the electrode assembly is rotatably connected with the base and used for avoiding the accommodating groove or extending into the electrophoresis box.

Further, the agarose gel electrophoresis device further comprises two rotating shafts, the two rotating shafts are horizontally arranged on the base and are respectively located at two sides of the electrophoresis box, and the plurality of positive pole pieces and the plurality of negative pole pieces are respectively and rotatably connected with the two rotating shafts.

Further, one end of the positive electrode piece and/or the negative electrode piece is provided with a sleeve, and the sleeve is sleeved on the rotating shaft.

Further, the end of the rotating shaft is provided with a power interface, and the power interface is electrically connected with the plurality of positive pole pieces and/or the plurality of negative pole pieces.

Further, the positive electrode piece and/or the negative electrode piece comprise an electrode carrier plate and electrode wires, the electrode wires are arranged on the electrode carrier plate, the electrode carrier plate is provided with a bending section which is bent towards the accommodating groove, and at least one part of the bending section stretches into the electrophoresis box.

Further, the agarose gel electrophoresis device further comprises a controller, and the controller is electrically connected with the plurality of electrode assemblies.

Further, agarose gel electrophoresis apparatus still includes the system subassembly of gluing, and the system subassembly of gluing includes: the base is provided with a glue making groove; the glue support piece is accommodated in the glue making groove and used for preparing agarose gel, and when agarose gel electrophoresis is carried out, the glue support piece is placed in the electrophoresis box.

Further, one side of the base is provided with a glue injection port and a diversion platform, the glue injection port is horn-shaped, and the diversion platform is arranged in a downward inclined manner and is sequentially connected with the glue injection port and the glue making groove respectively.

Further, the rubber support piece comprises a main body part and two handles, wherein the main body part is provided with a forming groove for accommodating agarose gel, the notch height of the forming groove is higher than the height of the flow guide platform of the base, and the two handles are arranged on two sides of the main body part in a staggered mode.

Further, the number of the glue support pieces is one or more, and when the number of the glue support pieces is multiple, the glue support pieces are sequentially arranged along the length direction of the base.

Further, the agarose gel is a single-well agarose gel.

By applying the technical scheme of the invention, the agarose gel electrophoresis device comprises a base, electrophoresis boxes and electrode assemblies, wherein the base is provided with a containing groove, the electrophoresis boxes are used for containing agarose gel, the electrophoresis boxes are arranged in the containing groove at intervals, the electrode assemblies are arranged in a plurality, the electrode assemblies are arranged in one-to-one correspondence with the electrophoresis boxes, the electrode assemblies comprise positive electrode pieces and negative electrode pieces, the positive electrode pieces and the negative electrode pieces are respectively positioned at two sides of the electrophoresis boxes, and thus, a plurality of independent electrophoresis boxes and matched electrode assemblies are adopted to isolate each sample in a relatively independent space for electrophoresis, so that cross infection can not occur among each sample, the success rate of sample test is ensured, and the problem that cross infection is easy to occur when the sample is subjected to agarose gel electrophoresis in the prior art is solved. In addition, through adopting a plurality of independent electrophoresis boxes, every electrophoresis box can hold an agarose gel to select the quantity of agarose gel according to actual demand, can not cause extravagant to other agarose gels, and the electrophoresis buffer that every electrophoresis box used is also independent, because independent electrophoresis box is less relatively, the electrophoresis buffer that uses is also less relatively, has reduced the waste of electrophoresis buffer, also simple and convenient when changing and washing electrophoresis box.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. In the drawings:

FIG. 1 is a schematic view showing the structure of an angle of an agarose gel electrophoresis apparatus in one embodiment of the present invention;

fig. 2 is a schematic view showing the structure of an electrophoresis cassette in one embodiment of the present invention;

FIG. 3 is a schematic view showing the structure of an agarose gel electrophoresis apparatus at another angle in one embodiment of the present invention;

FIG. 4 shows a schematic structural view of a base in one embodiment of the present invention;

FIG. 5 shows a schematic structural view of a glue holder in an embodiment of the invention;

FIG. 6 shows a schematic structural view of a glue making assembly according to an embodiment of the invention;

FIG. 7 shows a sample strip graph obtained using the prior art;

FIG. 8 shows a sample strip obtained by using the agarose gel electrophoresis apparatus of the present invention.

Wherein the above figures include the following reference numerals:

10. a base; 11. a boss; 20. an electrophoresis box; 21. the accommodating concave part; 30. a positive electrode member; 31. a sleeve; 32. an electrode carrier plate; 33. an electrode wire; 40. a negative electrode member; 50. a partition plate; 60. a rotating shaft; 70. a power interface; 80. a base; 81. a glue making groove; 82. a glue injection port; 83. a diversion platform; 90. a glue support; 91. a main body portion; 911. a forming groove; 92. a handle; 100. agarose gel; 101. and (5) a sample adding hole.

Detailed Description

It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other. The invention will be described in detail below with reference to the drawings in connection with embodiments.

It is noted that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless otherwise indicated.

In the present invention, unless otherwise indicated, terms of orientation such as "upper, lower, top, bottom" are used generally with respect to the orientation shown in the drawings or with respect to the component itself in the vertical, upright or gravitational direction; also, for ease of understanding and description, "inner and outer" refers to inner and outer relative to the profile of each component itself, but the above-mentioned orientation terms are not intended to limit the present invention.

It will be apparent that the embodiments described above are merely some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.

In order to solve the problem that cross infection is easy to occur when a sample is subjected to agarose gel electrophoresis in the prior art, the invention provides an agarose gel electrophoresis device.

As shown in fig. 1 to 3, the agarose gel electrophoresis apparatus includes a base 10, an electrophoresis cassette 20, and an electrode assembly. The base 10 has a receiving groove. The electrophoresis cassette 20 is used for accommodating agarose gel 100, the electrophoresis cassette 20 is a plurality of electrophoresis cassettes 20, and a plurality of electrophoresis cassettes 20 are arranged in the accommodating groove at intervals. The electrode assemblies are a plurality of, and a plurality of electrode assemblies and a plurality of electrophoresis boxes 20 are arranged in a one-to-one correspondence, and each electrode assembly comprises a positive electrode piece 30 and a negative electrode piece 40, and the positive electrode piece 30 and the negative electrode piece 40 are respectively positioned on two sides of the electrophoresis box 20.

The agarose gel electrophoresis apparatus includes a base 10, an electrophoresis cassette 20, and an electrode assembly. The base 10 is provided with a containing groove, the electrophoresis box 20 is used for containing agarose gel 100, a plurality of electrophoresis boxes 20 are arranged, and a plurality of electrophoresis boxes 20 are arranged in the containing groove at intervals; the electrode components are multiple, a plurality of electrode components and a plurality of electrophoresis boxes 20 are arranged in a one-to-one correspondence, the electrode components comprise a positive electrode component 30 and a negative electrode component 40, the positive electrode component 30 and the negative electrode component 40 are respectively positioned on two sides of the electrophoresis boxes 20, thus, the multiple independent electrophoresis boxes 20 and matched electrode components are adopted, each sample is isolated in a relatively independent space for electrophoresis, cross infection can not occur between each sample, thus, the success rate of sample test is ensured, in addition, each electrophoresis box 20 can accommodate one agarose gel 100, the quantity of agarose gel 100 is selected according to actual demands, waste is not caused to other agarose gels, and the electrophoresis buffer used by each electrophoresis box 20 is also independent.

In this embodiment, since the electrophoresis cassette 20 is independently provided, the agarose gel 100 is a single-well agarose gel such that one agarose gel 100 corresponds to one sample.

As shown in fig. 1 and 3, the middle of the bottom of the accommodating groove is provided with a boss 11, the boss 11 extends along the arrangement direction of the plurality of electrophoresis cassettes 20, and the bottom of the electrophoresis cassette 20 is provided with an accommodating recess 21 which is matched with the boss 11. When electrophoresis is performed, the accommodating concave part 21 at the bottom of the electrophoresis box 20 is clamped and embedded on the boss 11 at the bottom of the accommodating groove, so that the electrophoresis box is relatively fixed with the base 10. It can be understood that, since the middle of the bottom of the accommodating groove is provided with the boss 11, the accommodating groove is in an inverted concave shape, and correspondingly, the electrophoresis box 20 is also in an inverted concave shape, and the positive electrode member 30 and the negative electrode member 40 respectively extend into the grooves on two sides of the electrophoresis box 20.

As shown in FIG. 1, the agarose gel electrophoresis apparatus further includes a partition 50. The plurality of partition boards 50 are arranged on the boss 11 at intervals along the arrangement direction of the plurality of electrophoresis cassettes 20, and two adjacent partition boards 50 form a clamping groove for limiting the electrophoresis cassettes 20. Through the above arrangement, the partition 50 divides the boss 11 into a plurality of slots, so that the plurality of electrophoresis cassettes 20 can correspond to the plurality of slots, and the bottom surface portion of the accommodation recess 21 of the electrophoresis cassette 20 is accommodated in the clamping groove, thereby fixing the electrophoresis cassette 20 with respect to the base 10.

In this embodiment, the electrode assembly is rotatably connected to the base 10 for avoiding the receiving groove or extending into the electrophoresis cassette 20. Specifically, as shown in fig. 1 and 3, the agarose gel electrophoresis apparatus further includes two rotating shafts 60, the two rotating shafts 60 are horizontally disposed on the base 10 and are respectively located at two sides of the electrophoresis box 20, and the plurality of positive electrode members 30 and the plurality of negative electrode members 40 are respectively rotatably connected with the two rotating shafts 60. Fig. 3 shows the positions of the electrode assembly in the operating state and the non-operating state, when the electrode assembly is in the operating state, the positive electrode member 30 and the negative electrode member 40 are rotated into the receiving grooves, respectively, so as to protrude into the electrophoresis cassette 20. The method comprises the steps of carrying out a first treatment on the surface of the When the electrode assembly is in the non-operating state, the positive electrode member 30 and the negative electrode member 40 are both rotated upward to avoid the receiving groove, so that an experimenter can conveniently perform other operations such as placing the electrophoresis cassette 20.

As shown in fig. 1, one end of the positive electrode member 30 is provided with a sleeve 31, and the sleeve 31 is sleeved on the rotating shaft 60, so that the positive electrode member 30 can rotate around the rotating shaft 60.

As shown in fig. 1, the end of the rotating shaft 60 has a power interface 70, and the power interface 70 is electrically connected to the plurality of positive electrode members 30. The agarose gel electrophoresis apparatus further includes a power supply assembly electrically connected to the power interface 70 to supply power to the electrode assembly.

As shown in fig. 1, the positive electrode member 30 includes an electrode carrier plate 32 and electrode wires 33. The electrode wire 33 is disposed on the electrode carrier plate 32. The electrode carrier 32 has a bending section bent toward the accommodating groove, and at least a part of the bending section extends into the electrophoresis cassette 20. Specifically, the sleeve 31 is disposed at one end of the electrode carrier 32, and the electrode wire 33 is disposed at one end of the electrode carrier 32 away from the sleeve 31, i.e. one end close to the accommodating groove, so as to ensure that the electrode carrier 32 can be fully contacted with the electrophoresis buffer in the electrophoresis cassette 20.

It is understood that the relevant arrangement form of the negative electrode member 40 is identical to that of the positive electrode member 30 described above, and the negative electrode member 40 and the positive electrode member 30 are symmetrically arranged with respect to the electrophoresis cassette 20 or the boss 11, which will not be described herein.

In this embodiment, the agarose gel electrophoresis apparatus further includes a controller electrically connected to each of the plurality of electrode assemblies. Specifically, the controller comprises a plurality of on-off switches, and the on-off switches are arranged in one-to-one correspondence with the electrode assemblies, so that the on-off of the electrode assemblies is controlled respectively. Through the arrangement, the on/off of the electrode assemblies with the corresponding quantity can be controlled according to the quantity of the samples, and other electrode assemblies are in an inactive state, so that electric energy waste is avoided.

As shown in fig. 4 to 6, the agarose gel electrophoresis apparatus further comprises a gel-making assembly. The glue assembly includes a base 80 and a glue carrier 90. The base 80 has a glue groove 81. The gel holder 90 is accommodated in the gel preparation tank 81 for preparing the agarose gel 100. In performing agarose gel electrophoresis, a tray 90 is placed within the electrophoresis cassette 20, as shown in FIG. 3.

As shown in fig. 4 and 6, one side of the base 80 is provided with a glue injection port 82 and a diversion platform 83, wherein the glue injection port 82 is horn-shaped, so that the pouring of agarose gel liquid is facilitated, the diversion platform 83 is arranged obliquely downwards and is sequentially connected with the glue injection port 82 and the glue making groove 81 respectively, that is, agarose gel liquid sequentially flows into the glue making groove 81 through the glue injection port 82 and the diversion platform 83.

As shown in fig. 5, the glue holder 90 includes a main body portion 91 and a handle 92. The main body 91 has a forming groove 911 for accommodating the agarose gel 100, and the notch of the forming groove 911 is higher than the height of the diversion platform 83 of the base 80, so that when the liquid level of the agarose gel liquid in the forming groove 911 is level with the height of the diversion platform 83, the preset height of the agarose gel 100 is reached, and no additional cutting adjustment is needed for the agarose gel 100. The number of handles 92 is two, and the two handles 92 are alternately arranged on both sides of the main body 91. In this embodiment, the length of the molding groove 911 is adapted to the width of the glue producing groove 81, and after the glue support 90 is placed in the glue producing groove 81, agarose gel liquid flows into the molding groove 911 of the glue support 90 directly when flowing into the glue producing groove 81.

In this embodiment, the number of the glue holders 90 is one or more, and when the number of the glue holders 90 is plural, the glue holders 90 are sequentially arranged along the length direction of the base 80.

The following describes the use procedure of the agarose gel electrophoresis apparatus in this embodiment:

firstly, the glue support pieces 90 are placed in the glue making groove 81, and one glue support piece 90 or a plurality of glue support pieces 90 can be placed independently according to experimental requirements. The dissolved agarose gel liquid is slowly poured into the glue injection port 82, and the agarose gel liquid is uniformly distributed in each glue support 90 through the diversion platform 83 by utilizing the liquid communicating vessel principle, and the filled agarose gel liquid is not higher than the diversion platform 83. After comb teeth are inserted on one side of the gel making tank 81, the gel making assembly is placed in a horizontal and cool place to wait for the agarose gel liquid to cool and solidify. After removal of the comb teeth, the cooled and solidified agarose gel 100 has a single-well loading well 101 at one end, as shown in FIG. 3.

Positive and negative power lines of the power supply assembly are respectively connected to the power supply interface 70 on the base 10, the electrode assembly is lifted up to be in a non-working state, and then the electrophoresis cassette 20 is clamped into the baffle clamping groove on the boss 11. The prepared agarose gel 100 is taken out from the gel preparation tank 81 together with the gel support 90 and placed in the electrophoresis box 20, and the gel support 90 is positioned at the middle bulge platform of the electrophoresis box 20. An appropriate amount of running buffer is added to running box 20 such that running buffer is completely clear of agarose gel 100. The sample to be electrophoresed is slowly added to the well 101. The electrode carrier plate 32 corresponding to the position of the electrophoresis cassette 20 is rotated downward, so that the electrode wire 33 thereon is completely immersed in the electrophoresis buffer solution, and the electrode assembly is put into an operating state. And the controller is used for controlling the corresponding on-off switch to be turned on, and after the current, the voltage and the duration of the power supply assembly are set, the power supply assembly is turned on until the electrophoresis is finished. Taking out agarose gel 100 after electrophoresis, cutting gel, cutting required strips, and recovering gel to obtain the required product.

TABLE 1 comparison Table of results of prior art and sample raw letter detection of this example

Type(s)	Parameters (parameters)	Mouse tissue 1	Mouse tissue 2	Mouse tissue 3
					Prior Art	Others	0.051446945	0.018649518	0.095176849
This embodiment	Others	0.000643087	0.000643087	0.000643087
					Type(s)	Parameters (parameters)	Pork tissue 1	Pork tissue 2	Pork tissue 3
Prior Art	Others	0.178778135	0.035369775	0.493890675
					This embodiment	Others	0.000643087	0.000643087	0.000643087

Fig. 7 to 8 show sample strip charts in the prior art and the present example, respectively, corresponding to three mouse tissue samples and three pork tissue samples in table 1. As can be seen from fig. 7 to 8, the agarose gel electrophoresis apparatus of the present example was used for electrophoresis which was not significantly different from the prior art, that is, the test results were accurate. In Table 1, the other values represent the degrees, and the larger the values, the more serious the contamination, and it can be seen from Table 1 that the agarose gel electrophoresis apparatus of the present example has a good contamination prevention effect.

From the above description, it can be seen that the above embodiments of the present invention achieve the following technical effects: the agarose gel electrophoresis apparatus includes a base 10, an electrophoresis cassette 20, and an electrode assembly. The base 10 is provided with a containing groove, the electrophoresis box 20 is used for containing agarose gel 100, a plurality of electrophoresis boxes 20 are arranged, and a plurality of electrophoresis boxes 20 are arranged in the containing groove at intervals; the electrode components are multiple, a plurality of electrode components and a plurality of electrophoresis boxes 20 are arranged in a one-to-one correspondence, the electrode components comprise a positive electrode component 30 and a negative electrode component 40, the positive electrode component 30 and the negative electrode component 40 are respectively positioned on two sides of the electrophoresis boxes 20, thus, the multiple independent electrophoresis boxes 20 and matched electrode components are adopted, each sample is isolated in a relatively independent space for electrophoresis, cross infection can not occur between each sample, thus, the success rate of sample test is ensured, in addition, each electrophoresis box 20 can accommodate one agarose gel 100, the quantity of agarose gel 100 is selected according to actual demands, waste is not caused to other agarose gels, and the electrophoresis buffer used by each electrophoresis box 20 is also independent.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.

It should be noted that the terms "first," "second," and the like in the description and claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that embodiments of the present application described herein may be implemented in sequences other than those illustrated or described herein.

The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (14)

1. An agarose gel electrophoresis apparatus, comprising:

a base (10), wherein the base (10) is provided with a containing groove;

the electrophoresis box (20) is used for accommodating agarose gel (100), the electrophoresis boxes (20) are a plurality of, and the electrophoresis boxes (20) are arranged in the accommodating groove at intervals;

the electrophoresis box comprises a plurality of electrophoresis boxes (20), wherein the electrophoresis boxes are arranged in a one-to-one correspondence mode, the electrophoresis boxes are arranged in a plurality of electrode assemblies, each electrode assembly comprises a positive electrode piece (30) and a negative electrode piece (40), and the positive electrode pieces (30) and the negative electrode pieces (40) are respectively arranged on two sides of the electrophoresis boxes (20).

2. Agarose gel electrophoresis apparatus according to claim 1, wherein the middle part of the bottom of the accommodation groove is provided with a boss (11), the boss (11) extends along the arrangement direction of a plurality of electrophoresis cassettes (20), and the bottom of the electrophoresis cassette (20) is provided with an accommodation concave part (21) which is matched with the boss (11).

3. Agarose gel electrophoresis apparatus according to claim 2, further comprising a plurality of partitions (50), wherein a plurality of partitions (50) are vertically arranged on the boss (11) and are arranged at intervals along the arrangement direction of the plurality of electrophoresis cassettes (20), and two adjacent partitions (50) form a clamping groove for limiting the electrophoresis cassettes (20).

4. Agarose gel electrophoresis apparatus according to claim 1, wherein the electrode assembly is rotatably connected to the base (10) for avoiding the receiving recess or extending into the electrophoresis cassette (20).

5. Agarose gel electrophoresis apparatus according to claim 4 further comprising two shafts (60), two shafts (60) being horizontally disposed on the base (10) and being located on both sides of the electrophoresis cassette (20), a plurality of the positive electrode members (30) and a plurality of the negative electrode members (40) being rotatably connected with the two shafts (60), respectively.

6. Agarose gel electrophoresis apparatus according to claim 5 wherein the positive electrode member (30) and/or the negative electrode member (40) has a sleeve (31) at one end, the sleeve (31) being sleeved on the rotating shaft (60).

7. Agarose gel electrophoresis apparatus according to claim 5, wherein the end of the spindle (60) has a power interface (70), the power interface (70) being electrically connected to a plurality of the positive members (30) and/or a plurality of the negative members (40).

8. Agarose gel electrophoresis apparatus according to claim 1, wherein the positive element (30) and/or the negative element (40) comprises an electrode carrier plate (32) and a wire electrode (33), the wire electrode (33) being arranged on the electrode carrier plate (32), the electrode carrier plate (32) having a bending section bending towards the receiving groove, at least a portion of the bending section extending into the electrophoresis cassette (20).

9. The agarose gel electrophoresis apparatus according to any one of claims 1 to 8, further comprising a controller electrically connected to each of the plurality of electrode assemblies.

10. The agarose gel electrophoresis apparatus according to any one of claims 1 to 8, further comprising a gel-making assembly comprising:

a base (80), wherein the base (80) is provided with a glue making groove (81);

the gel support piece (90), the support piece (90) holds in making gluey groove (81), be used for preparing agarose gel (100), when carrying out agarose gel electrophoresis, support piece (90) is placed in electrophoresis box (20).

11. Agarose gel electrophoresis apparatus according to claim 10, wherein a glue injection port (82) and a diversion platform (83) are provided on one side of the base (80), the glue injection port (82) is horn-shaped, and the diversion platform (83) is arranged obliquely downwards and is sequentially connected with the glue injection port (82) and the glue making groove (81) respectively.

12. Agarose gel electrophoresis apparatus according to claim 10, wherein the gum support (90) comprises a main body (91) and handles (92), the main body (91) has a forming groove (911) for accommodating the agarose gel (100), the notch of the forming groove (911) is higher than the guide platform (83) of the base (80), the handles (92) are two, and the two handles (92) are staggered on both sides of the main body (91).

13. Agarose gel electrophoresis apparatus according to claim 10, wherein the number of the gel holders (90) is one or more, and when the number of the gel holders (90) is plural, the plurality of the gel holders (90) are sequentially arranged along the length direction of the base (80).

14. Agarose gel electrophoresis apparatus according to claim 10 wherein the agarose gel (100) is a single-well agarose gel.

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