CN116369521A - A composition capable of promoting intestinal health - Google Patents

Abstract

The invention provides a composition with an effect of promoting intestinal health, and belongs to the field of biological medicines. The composition is composed of sesamin powder, ginger essential oil and attapulgite powder, has the functions of improving intestinal dysfunction by the sesamin and ginger essential oil, protecting and repairing intestinal mucosa by the attapulgite, and has the functions of synergism and promotion of intestinal health. Pharmacological tests show that the sesamin powder, ginger essential oil and attapulgite powder composition has the functions of regulating intestinal functions, protecting intestinal mucosa, resisting bacteria and inflammation and promoting intestinal health; the combination of sesamin, ginger essential oil and attapulgite is obviously superior to single use or the combination of the sesamin, ginger essential oil and attapulgite, and has synergistic effect. Toxicological tests have shown that the compositions of the invention are safe to use.

Description

A composition capable of promoting intestinal health

technical field

The invention belongs to the field of biomedicine, and in particular relates to a composition capable of promoting intestinal health.

Background technique

Intestinal function directly affects people's digestion and absorption of nutrients in food, and is very important for maintaining the normal functions of other organs of the human body. Intestinal dysfunction caused by diarrhea, gastrointestinal dysfunction, chronic inflammation, immune dysfunction, etc. will bring suffering to patients and damage intestinal health and human health. At present, the drugs used to prevent and treat intestinal disorders such as diarrhea are mostly antidiarrheals, antibacterial and anti-inflammatory drugs, which have problems such as large side effects and easy drug resistance. In the prior art, there is a lack of new oral preparations that are derived from natural sources, have significant effects, and have no obvious adverse reactions to improve intestinal function and promote intestinal health, so as to alleviate the pain of patients.

Sesamin is the main functional component in sesame seeds, which belongs to lignans; sesamin has various physiologically active functions such as anti-oxidation, lowering blood lipids (serum cholesterol, triglycerides), protecting liver and kidney functions, and can be used in medicine and Health functional food field.

Ginger is a traditional health food resource with the same source of medicine and food. It has multiple functions such as invigorating the stomach and warming the stomach, dispelling cold and dampness, relaxing tendons and activating blood circulation, anti-inflammatory and antibacterial. Ginger essential oil is a volatile substance extracted from the rhizome of ginger. It is a brown transparent oily liquid. It has pharmacological effects such as promoting blood circulation and removing blood stasis, antibacterial and anti-inflammatory. It is widely used in food, health food, cosmetics and pharmaceutical fields.

Attapulgite is a clay mineral with a layered chain structure of hydrous magnesium aluminum silicate. It has a unique microstructure and charging properties, and has a variety of excellent properties, such as adsorption and colloidal properties. It is widely used in medicine, Chemical industry, agriculture and environmental protection fields.

In the prior art, there is no report on the combination of sesamin, ginger essential oil and attapulgite for improving intestinal dysfunction and promoting intestinal health.

Contents of the invention

The purpose of the present invention is to provide a composition with the effect of promoting intestinal health, which is a composition composed of sesamin powder, ginger essential oil and attapulgite powder, which not only has the functions of sesamin and ginger essential oil to improve intestinal dysfunction, but also The attapulgite protects and repairs the function of the intestinal mucosa, and the three have a synergistic effect on promoting intestinal health.

The mass ratio of the sesamin, ginger essential oil and attapulgite powder is 0.1-2:0.1-2:10.

The sesamin is extracted from sesame meal, wherein the sesamin content is greater than 50%; the ginger essential oil is extracted from ginger, wherein the gingerol content is greater than 20%; the attapulgite powder is selected to have an attapulgite crystal content greater than 50%, Attapulgite clay ore type with carbonate content less than 10%, grind the attapulgite clay ore to 100-320 mesh. The composition can be processed into oral preparations according to known techniques, such as granules, tablets, capsules, soft capsules and the like.

Pharmacological tests show that the sesamin powder, ginger essential oil, and attapulgite powder composition of the present invention have the effects of regulating intestinal function, protecting intestinal mucosa, antibacterial and anti-inflammatory, and promoting intestinal health; the combination of sesamin + ginger essential oil + attapulgite Combination is obviously better than single use or combination of both, and has synergistic effect. Toxicological tests show that the composition of the invention is safe to use.

Detailed ways

The present invention will be further described below in conjunction with embodiment.

Example 1

A composition capable of promoting intestinal health is composed of sesamin powder extracted from sesame meal, ginger essential oil extracted from ginger, and attapulgite powder, and the ratio of the three is 1:1:10.

(1) Preparation of Sesamin Extract

The preparation method of sesamin extract: grind the sesame meal into powder, add n-hexane twice the volume of the sesame meal powder, stir for 3 hours to degrease, and then use the same method for the second degreasing, after filtering, air-dry to obtain degreasing Sesame meal. According to the ratio of adding 8.5ml of 93% ethanol aqueous solution per gram of defatted sesame meal, add 93% ethanol aqueous solution to the defatted sesame meal, stir in a water bath at 55°C, extract for 2.5h, filter, and separate the extract and defatted sesame meal , and then use the same method to repeat the extraction of defatted sesame meal once. Combine the two extracts, filter, evaporate part of the ethanol from the filtrate on a rotary evaporator, concentrate under reduced pressure, and vacuum dry at 60°C to obtain a sesamin extract, wherein the content of sesamin is 698.07mg/g. The properties of this product are: light brown powder, odorless, tasteless, hygroscopic, soluble in alcohol, insoluble in water.

Wherein the determination method of sesamin content is as follows: adopt HPLC method. The sesamin standard solution is between 20-100 μg/ml, and the corresponding peak area has a significant linear relationship with the sesamin mass concentration, and a standard curve is made. Dissolve the sesamin extract with chromatographically pure absolute ethanol, and filter. Column temperature: room temperature; mobile phase: methanol-water (8:2, v/v); flow rate: 0.8ml/min; injection volume: 20μl; detection wavelength: 290nm; analysis time: 15min. According to the retention time of the standard sample, the content of sesamin was calculated by the external standard method.

(2) Preparation of Ginger Essential Oil

The preparation method of ginger essential oil is as follows: ginger is peeled, washed and sliced, dried and pulverized to obtain ginger powder. Add 70% ethanol solution to the ginger powder for reflux extraction twice, add 70% ethanol solution with 10 times the volume of ginger powder in the first extraction, and extract for 90 minutes; add 70% ethanol solution with 8 times the volume of ginger powder in the second extraction , extracted for 60 min, combined ethanol extracts, filtered, and the filtrate was concentrated under reduced pressure and evaporated to exhaust ethanol to obtain ginger essential oil. The properties of this product are: light yellow oily substance with pungent taste, soluble in alcohol, insoluble in water. The content of gingerol in ginger essential oil is 246.25mg/g.

Wherein, the determination method of gingerol content is as follows: adopt HPLC method; Dissolve ginger essential oil with chromatographically pure absolute ethanol, filter. Column temperature: room temperature; mobile phase: acetonitrile-methanol-water (40:5:55); flow rate: 0.8ml/min; injection volume: 20μl; detection wavelength: 280nm. According to the retention time of the standard sample, the content of gingerol was calculated by the external standard method.

(3) Activation of attapulgite

Select the attapulgite clay ore type with attapulgite crystal content greater than 50% and carbonate content less than 10%. Take the attapulgite powder ground to 200 mesh, put it into a beaker filled with 20% hydrochloric acid by mass, seal it with a plastic film, and stir it magnetically in a water bath at 60°C for 10 hours. Then, the supernatant was removed by centrifugation at 10,000 rpm, and the precipitate was washed with deionized water for several times until the pH of the supernatant was neutral and the color was transparent. Add the precipitate after washing with water to 10% (mass percent concentration) sodium chloride aqueous solution, and ultrasonically form a suspension system; then, stir magnetically in a water bath at 60°C for 10 hours; centrifuge at 10,000rpm, take the upper layer of light gray solid, and blow it with electric heating and constant temperature Dry to constant weight at 70°C in a drying oven to obtain activated attapulgite.

Example 2

This example uses the sesamin extract, ginger essential oil and activated attapulgite prepared in Example 1 to carry out animal experiments to investigate how the sesamin extract, ginger essential oil and activated attapulgite regulate intestinal function and protect intestinal mucosa , antibacterial and anti-inflammatory, and promote intestinal health. The sesamin used in this example is added in the form of a sesamin extract, and the mass of the sesamin extract used is calculated according to the sesamin content in the sesamin extract being 698.07 mg/g.

Preparation of Escherichia coli bacterial suspension: inoculate Escherichia coli in LB medium, shake culture at 37°C for 12 hours, centrifuge the Escherichia coli culture solution to obtain the bacterial cell precipitate, wash the bacterial cell precipitate with sterilized normal saline twice, and then use it after centrifugation Bacterial saline to make 2×10 ¹⁰ CFU/mL E. coli suspension and store at 4°C for later use.

To establish an animal diarrhea model infected with Escherichia coli: C ₅₇ BL/6J mice were fasted for 12 hours before the experiment and had free access to water. Each mouse was intraperitoneally injected with Escherichia coli solution 0.5mL, and when the mice had obvious symptoms of diarrhea, watery stool, listlessness, sluggishness and other symptoms, it indicated that the Escherichia coli infection animal diarrhea model was successfully established; Rats were intraperitoneally injected with an equal volume of sterile saline.

Experimental design: 108 6-week-old SPF grade C ₅₇ BL/6J mice (half male and half male). All mice were acclimatized for 3 days in an SFP grade animal room with constant temperature and humidity, controlled 12h light/dark, and the ambient temperature was 25%, during which the mice had free access to food and water. After the adaptation, the mice were randomly divided into 9 groups (12 in each group, half male and half male), respectively normal control group, model control group, sesamin (ZMS, 60mg·kg ^-1 ·d ^-1 ) group, ginger essential oil (GEO, 60mg·kg ^-1 ·d ^-1 ) group, activated attapulgite (PGS, 60mg·kg ^-1 ·d ^-1 ) group, ZMS+GEO (30+30mg·kg ^-1 ·d ^-1 ) group, ZMS+PGS (30+30mg·kg ^-1 ·d ^-1 ) group, GEO+PGS (30+30mg·kg ^-1 ·d ^-1 ) group, ZMS+GEO+PGS (20 +20+20 mg·kg ^-1 ·d ^-1 ) group. Continuous administration for 6 days.

The detection indicators include: the feed is weighed and replaced once a day, and the average food intake of mice is calculated. Mouse body weights were recorded daily. During the experiment, the mental state, gastrointestinal reaction, movement and reaction ability of the mice were observed. Diarrhea index: Put the mice into a mouse cage covered with filter paper, observe the diarrhea of the mice; calculate the diarrhea rate.

The results of the animal experiments are shown in Tables 1-3 below.

Table 1 Effects on mouse body weight

Note: In Table 1, if a certain group is given multiple medicines, the numbers in the corresponding dose column are added together, and the positions of the numbers correspond to the medicines in the administration column. For example, in the ZMS+GEO group, dose one "30+30" in the column means the administration of sesamin 30mg.kg.d ^-1 and ginger essential oil 30mg.kg.d ^-1 , the same below; compared with the normal control group, " ^* " means P<0.05, " ^{* *} "means P<0.01; compared with the model control group, "^#" means P<0.05, "^##" means P<0.01.

Table 2 Effects on the food intake of mice

Note: In Table 2, compared with the normal control group, " ^* " means P<0.05, " ^** " means P<0.01; compared with the model control group, "^#" means P<0.05, "^##" means P<0.01.

The influence of table 3 on mouse diarrhea rate

The results showed that, compared with the normal control group, the body weight and food intake of the mice in the model control group were significantly reduced, and the diarrhea rate was significantly increased. Compared with the model control group, the intervention of sesamin (ZMS), ginger essential oil (GEO), and attapulgite (PGS) can significantly restore the weight and food intake of mice with diarrhea, reduce the rate of diarrhea, and promote the recovery of mice. It shows that sesamin (ZMS), ginger essential oil (GEO), and attapulgite (PGS) have the functions of regulating intestinal function, antibacterial and anti-inflammation, protecting intestinal mucosa, and promoting intestinal health; the combination of sesamin, ginger essential oil and attapulgite It is obviously better than single use or combination of both, and has synergistic effect. The above results show that the combination of sesamin, ginger essential oil and attapulgite has a synergistic effect of enhancing drug efficacy.

The results of the acute toxicity test of the present invention show that the maximum administration dose (MTD) of sesamin (ZMS)+ginger essential oil (GEO)+attapulgite (PGS) in mice is 22.9g/kg, which is equivalent to 70 times the effective dose. ; Animals did not have any adverse reactions. It shows that the product of the present invention is safe and has almost no toxic and side effects.

Example 3

In this example, the sesamin extract, ginger essential oil and activated attapulgite prepared in Example 1 were used to prepare various dosage forms of drugs for promoting intestinal health. The sesamin used in this example is added in the form of a sesamin extract, and the mass of the sesamin extract used is calculated according to the sesamin content in the sesamin extract being 698.07 mg/g.

Tablets containing sesamin extract, ginger essential oil and activated attapulgite, each containing 60mg sesamin, 120mg ginger essential oil and 600mg attapulgite. Mix the sesamin, ginger essential oil and attapulgite evenly, and prepare tablets according to a conventional method.

Powder containing sesamin extract, ginger essential oil and activated attapulgite, each bag contains 120mg sesamin, 60mg ginger essential oil and 600mg attapulgite. Mix the sesamin, ginger essential oil and attapulgite evenly, and prepare the powder according to the conventional method.

Capsules containing sesamin extract, ginger essential oil and activated attapulgite, each containing 60mg sesamin, 30mg ginger essential oil and 600mg attapulgite. Mix the sesamin, ginger essential oil and attapulgite evenly, and prepare capsules according to a conventional method.

Daily oral dose, in terms of effective part composition, the total mass of sesamin, ginger essential oil and attapulgite is about 700mg, which is used to regulate and improve intestinal function and promote intestinal health (such as chronic intestinal inflammation, functional dyspepsia, Intestinal dysfunction caused by irritable bowel syndrome, etc.), the method of taking the powder is 2 times a day, 1 bag each time, washed with warm water; the method of taking the tablet is 2 times a day, 1-2 times a day Tablets; Capsules are taken twice a day, 1-2 capsules each time. 1 month is a course of treatment.

Claims (6)

1. The composition with the effect of promoting intestinal health is characterized by comprising sesamin, ginger essential oil and attapulgite, wherein the mass ratio of sesamin to ginger essential oil to attapulgite is 0.1-2:0.1-2: 10-20.

2. The composition of claim 1, wherein the sesamin is extracted from sesame seed meal, wherein the sesamin content is greater than 50%.

3. Composition according to claim 1 or 2, wherein the ginger essential oil is extracted from ginger with a gingerol content of more than 20%.

4. A composition according to claim 3, wherein the attapulgite has a rod crystal content of greater than 50% and a carbonate content of less than 10%.

5. The composition of claim 4, wherein the attapulgite is ground to a powder of 100-320 mesh.

6. The composition of claim 5, wherein sesamin, ginger essential oil and attapulgite are mixed uniformly, and mixed with excipient to make powder, granule, tablet or capsule.