CN116359524B

CN116359524B - Sample detection device

Info

Publication number: CN116359524B
Application number: CN202310634792.7A
Authority: CN
Inventors: 邓灵强
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2023-05-31
Filing date: 2023-05-31
Publication date: 2023-09-15
Anticipated expiration: 2043-05-31
Also published as: CN116359524A

Abstract

The application discloses a sample detection device, which comprises at least two sample liquid reaction units, a sample liquid conveying main pipe, a first power device, a second power device, a focusing liquid receiving part and a sample liquid receiving part. The first power device conveys the sample liquid from one of the at least two sample liquid reaction units into the first sub-pipe section of the sample liquid conveying main pipe, and the second power device pushes the sample liquid in the first sub-pipe section into the sample liquid receiving piece. The inner diameter range of the sample liquid receiving part is 0.16-0.24mm; and/or the sample liquid receiver is 60-100mm in length; and/or the roughness of the inner wall of the sample liquid receiving member is 0.4-1.6Ra. Thereby increase the pipe resistance of sample liquid receiver, avoided focusing fluid refluence to enter in the sample liquid receiver, caused reagent pollution to the sample liquid receiver, improved sample detection device's security.

Description

Sample detection device

Technical Field

The present application relates to the field of sample analysis technologies, and in particular, to a sample detection device.

Background

The current sample detection device generally adopts a flow type laser scattering technology to analyze sample liquid, and the sample liquid passes through a light detection area in a laminar flow mode under the wrapping of a focusing liquid, so that cells in the sample liquid can pass through the light detection area one by one, and sample detection is completed in the light detection area.

However, because the focusing fluid is to wrap the sample fluid, the focusing fluid assembly is usually sleeved on the sample fluid assembly, and when the sample detection device switches to detect the next sample fluid, a period of idle time exists in the sample fluid assembly to wait for the injection of the next sample fluid, at this time, the focusing fluid in the focusing fluid assembly is easy to flow back into the sample fluid assembly, and reagent pollution is caused to the sample fluid assembly and a transmission pipeline connected with the sample fluid assembly, so that the safety of the sample detection device is reduced.

Disclosure of Invention

The present application provides a sample detection device to solve the above technical problems.

The sample detection device comprises at least two sample liquid reaction units, a sample liquid conveying main pipe, a first power device, a second power device, a focusing liquid receiving piece and a sample liquid receiving piece; the sample liquid conveying main pipe is connected with the sample liquid receiving piece and the at least two sample liquid reaction units;

the at least two sample liquid reaction units are used for preparing different sample liquids;

the sample liquid conveying main pipe is used for receiving the sample liquid prepared by the sample liquid reaction unit in a time-sharing mode and conveying the sample liquid to the sample liquid receiving piece;

The first power device and the second power device are respectively connected with the outflow end and the inflow end of the sample liquid conveying main pipe;

the sample liquid receiving part is connected with an access point of the sample liquid conveying main pipe, which is close to the outflow end;

the at least two sample liquid reaction units are sequentially connected with the access point of the sample liquid conveying main pipe along the direction from the inflow end to the outflow end of the sample liquid conveying main pipe;

a sub-pipe section on the sample liquid conveying main pipe between an access point of the sample liquid receiving part on the sample liquid conveying main pipe and an access point of the sample liquid reaction unit nearest to the sample liquid receiving part on the sample liquid conveying main pipe is a first sub-pipe section;

the first power device utilizes negative pressure to convey the sample liquid in the sample liquid reaction unit into the first sub-pipe section;

the second power device utilizes positive pressure to push the sample liquid in the first sub-pipe section to the sample liquid receiving piece;

the focusing liquid receiving part is sleeved outside the sample liquid receiving part and is used for receiving the sample liquid in the first sub-pipe section and injecting the sample liquid into the focusing liquid receiving part; the focusing liquid receiving piece is used for receiving the focusing liquid and the sample liquid and enabling the focusing liquid to wrap the sample liquid to form a focusing sample liquid laminar flow;

And the inner diameter of the sample liquid receiving part is 0.16-0.24mm; and/or the sample liquid receiver is 60-100mm in length; and/or the roughness of the inner wall of the sample liquid receiving member is 0.4-1.6Ra.

Wherein the sample detection device further comprises a first three-way joint;

the first power device, the sample liquid receiving part and the first sub-pipe section on the sample liquid conveying main pipe are respectively connected to the three ends of a first three-way joint, and an access point of the sample liquid receiving part and the sample liquid conveying main pipe is positioned between the outflow end and the access points of the sample liquid reaction unit and the sample liquid conveying main pipe;

the sub-pipe section of the sample liquid conveying main pipe between the outflow end and the access point of the sample liquid receiving piece on the sample liquid conveying main pipe is a second sub-pipe section,

wherein the inner diameter of the second sub-pipe section is larger than the inner diameter of the first sub-pipe section.

Wherein the capacity of the first sub-tube segment is larger than the maximum value of the sample volumes provided by the at least two sample liquid reaction units for one detection.

Wherein the sub-pipe section of the sample liquid conveying main pipe between the access points of the adjacent sample liquid reaction units on the sample liquid conveying main pipe is a third sub-pipe section

Wherein the inner diameter of the third sub-pipe section is larger than or equal to the inner diameter of the first sub-pipe section.

The sample liquid reaction unit is connected with the sample liquid conveying main pipe through a conveying branch pipeline, and the conveying branch pipeline comprises a first conveying branch pipeline, a two-way valve and a second conveying branch pipeline;

the sample liquid reaction unit is communicated with one end of the first conveying branch pipeline,

the other end of the first conveying branch pipeline is communicated with one end of the two-way valve,

the other end of the two-way valve is communicated with one end of the second conveying branch pipeline,

the other end of the second conveying branch pipeline is communicated with the sample liquid conveying main pipe through a first end of a second three-way joint;

wherein the length of the first conveying branch pipeline is smaller than a first set length,

and/or the length of the second conveying branch pipeline is smaller than the second set length.

Wherein a sub-pipe section of the sample liquid conveying main pipe between an access point of the second power device on the sample liquid conveying main pipe and an access point of the sample liquid reaction unit closest to the second power device on the sample liquid conveying main pipe is a fourth sub-pipe section,

Wherein the inner diameter of the fourth sub-pipe section is greater than or equal to the inner diameter of the third sub-pipe section.

Wherein the sample detection device further comprises a focusing liquid supply unit and a rectifying flow channel,

the focusing fluid supply unit is connected to the focusing fluid receiving member through the rectifying flow path,

the extension direction of the focusing fluid outlet of the rectifying flow channel is the same as the extension direction of the focusing fluid receiving part,

the extension direction of the focusing fluid inlet of the flow straightening channel is perpendicular to the extension direction of the focusing fluid outlet of the flow straightening channel,

wherein the driving pressure supplied by the focusing fluid supply unit is greater than a set pressure value.

The focusing liquid receiving part also comprises an acceleration flow channel and a detection flow channel, and the focusing liquid flows to the acceleration flow channel and the detection flow channel through the rectification flow channel;

the radial dimension of the flow straightening channel is larger than that of the detection channel, the flow accelerating channel is respectively communicated with the flow straightening channel and the detection channel, the radial dimension of the flow accelerating channel gradually decreases from one end of the flow accelerating channel connected with the flow straightening channel to the other end of the flow accelerating channel connected with the detection channel, and an included angle between the axial extending direction of the pipeline of the flow accelerating channel and the pipeline of the detection channel is smaller than 40 degrees.

Wherein the focusing fluid supply unit includes a first focusing fluid line and a second focusing fluid Jiao Ye line connected in parallel with each other;

the pipe diameter of the first focusing fluid pipeline is larger than that of the second focusing fluid pipeline Jiao Ye;

the first focusing fluid pipeline and the second focusing fluid pipeline can be independently conducted and closed;

the focusing fluid is supplied to the focusing fluid receiving member through the first focusing fluid line and/or the second focusing fluid line in an on state,

wherein the pipe diameter of the second focusing fluid pipeline is smaller than 1mm,

and the ratio of the pipe diameters of the first focusing liquid pipeline and the second focusing liquid pipeline is 2-8.

Wherein, the inner diameter of the tube of the first focusing fluid pipeline near one side of the focusing fluid receiving part is different from the inner diameter of the tube of the first focusing fluid pipeline far away from one side of the focusing fluid receiving part.

The application has the beneficial effects that: unlike the prior art, the sample detection device of the present application comprises at least two sample liquid reaction units, a sample liquid delivery main pipe, a first power device, a second power device, a focusing liquid receiving member, and a sample liquid receiving member. The first power device conveys the sample liquid from one of the at least two sample liquid reaction units into the first sub-pipe section of the sample liquid conveying main pipe, and the second power device pushes the sample liquid in the first sub-pipe section into the sample liquid receiving piece. The inner diameter range of the sample liquid receiving part is 0.16mm-0.24mm; and/or the sample liquid receiver is 60-100mm in length; and/or the roughness of the inner wall of the sample liquid receiving member is 0.4-1.6Ra. Thereby increase the pipe resistance of sample liquid receiver, avoided focusing fluid refluence to enter in the sample liquid receiver, caused reagent pollution to the sample liquid receiver, improved sample detection device's security.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:

FIG. 1 is a schematic view of a first embodiment of a sample testing device according to the present application;

FIG. 2 is a schematic diagram of a sample testing device according to a second embodiment of the present application;

FIG. 3 is a schematic structural view showing a first embodiment of a sample liquid reaction unit according to the present application;

FIG. 4 is a schematic diagram of a third embodiment of a sample testing device according to the present application;

FIG. 5 is a schematic view of a fourth embodiment of a sample testing device according to the present application;

fig. 6 is a schematic structural view of a first embodiment of the focus fluid supply unit of the present application.

Reference numerals: a sample detection device 1; a sample liquid reaction unit 10; a first transfer branch line 11; a two-way valve 12; a second transfer branch line 13; a second three-way joint 14; a sample liquid transporting main pipe 20; a first sub-section 21; a first three-way joint 22; a second sub-section 23; a third sub-segment 24; a fourth sub-segment 25; a first power device 31; a second power plant 32; a focusing fluid receiving member 40; an acceleration flow path 41; a detection flow path 42; a sample liquid receiving member 50; a focusing liquid supply unit 60; a flow rectifying passage 61; a first focusing fluid line 62; a second conduit Jiao Ye; a first direction X.

Detailed Description

The application is described in further detail below with reference to the drawings and examples. It is specifically noted that the following examples are only for illustrating the present application, but do not limit the scope of the present application. Likewise, the following examples are only some, but not all, of the examples of the present application, and all other examples, which a person of ordinary skill in the art would obtain without making any inventive effort, are within the scope of the present application.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.

In the description of the present application, it should be noted that, unless explicitly stated and limited otherwise, the terms "mounted," "disposed," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; the connection can be mechanical connection or electric connection; may be directly connected or may be connected via an intermediate medium. It will be apparent to those skilled in the art that the foregoing is in the specific sense of the present application.

Referring to fig. 1, fig. 1 is a schematic structural diagram of a sample detection device according to a first embodiment of the present application. The sample detection device 1 provided by the embodiment of the application comprises at least two sample liquid reaction units 10, a sample liquid conveying main pipe 20, a first power device 31, a second power device 32, a focusing liquid receiving part 40 and a sample liquid receiving part 50.

Wherein at least two sample liquid reaction units 10 are used for preparing different sample liquids, so that the sample detection device 1 can detect a plurality of sample liquids in one detection cycle. The sample liquid delivery main pipe 20 is connected to the sample liquid receiving member 50 and at least two sample liquid reaction units 10, and the sample liquid delivery main pipe 20 is used for receiving the sample liquid prepared by the sample liquid reaction units 10 in a time-sharing manner and for delivering the sample liquid to the sample liquid receiving member 50. That is, at least two sample liquid reaction units 10 may transmit the prepared sample liquid to the sample liquid receiving part 50 through the sample liquid transporting main pipe 20 at a time-sharing period, thereby performing sample detection on the sample liquid.

The first power device 31 and the second power device 32 are respectively connected to the outflow end and the inflow end of the sample fluid conveying main pipe 20; the sample liquid receiving part 50 is connected to an access point of the sample liquid transporting main pipe 20 near the outflow end, and at least two sample liquid reaction units 10 are sequentially connected to the access point of the sample liquid transporting main pipe 20 along the direction from the inflow end to the outflow end of the sample liquid transporting main pipe 20. And the sub-pipe section on the sample liquid transporting main pipe 20 between the access point of the sample liquid receiving element 50 on the sample liquid transporting main pipe 20 and the access point of the sample liquid reaction unit 10 nearest to the sample liquid receiving element 50 on the sample liquid transporting main pipe 20 is the first sub-pipe section 21.

In the present embodiment, the first power device 31 uses negative pressure to convey the sample liquid in the sample liquid reaction unit 10 into the first sub-tube segment 21; while the second motive device 32 uses positive pressure to push the sample fluid within the first sub-section 21 into the sample fluid receiver 50. The first power device 31 and the second power device 32 may be a negative air pressure source and a positive air pressure source of the same power source, so that the device cost of the sample detection device 1 is saved on the premise of realizing the function of the sample detection device 1. In another embodiment, the first power device 31 and the second power device 32 may be different power sources for improving the precision degree of the sample liquid delivery.

The focusing fluid receiving element 40 may be sleeved outside the sample fluid receiving element 50, where the sample fluid receiving element 50 is configured to receive the sample fluid in the first sub-tube segment 21 and inject the sample fluid into the focusing fluid receiving element 40; the focusing fluid receiving element 40 is configured to receive the focusing fluid and the sample fluid, and enable the focusing fluid to wrap the sample fluid to form a focused sample fluid laminar flow, where the focused sample fluid laminar flow includes a sample fluid laminar flow located at an inner layer and a focusing fluid laminar flow wrapped at an outer layer, and the focused fluid laminar flow is a laminar flow located at the outer layer relative to the sample fluid laminar flow of the inner layer.

In this embodiment, the focusing solution may be a sheath solution that does not affect cells in the sample solution, after the sample solution is injected into the focusing solution receiving element 40 by the sample solution receiving element 50, the sample solution is wrapped by the focusing solution in the focusing solution receiving element 40 to form a focused sample solution laminar flow, so that the cells in the sample solution are arranged one by one in the focused sample solution laminar flow, which is convenient for the sample detection device 1 to perform sample detection on the sample solution. The focusing fluid receiving member 40 is sleeved outside the sample fluid receiving member 50, so that the focusing fluid is convenient to wrap the sample fluid, and the detection efficiency of the sample detection device 1 is improved.

Further, the inner diameter of the sample liquid receiving member 50 is 0.16mm to 0.24mm; and/or the sample liquid receiver 50 is 60-100mm in length; and/or the roughness of the inner wall of the sample liquid receiving member 50 is 0.4 to 1.6Ra.

Wherein the smaller the inner diameter range of the sample liquid receiving member 50, the larger the tube resistance of the sample liquid receiving member 50 under the same pushing pressure; the longer the length of the sample liquid receiving member 50, the greater the tube resistance of the sample liquid receiving member 50; the greater the roughness of the inner wall of the sample liquid receiving member 50, the greater the tube resistance of the sample liquid receiving member 50; in the embodiment of the application, the device parameters of the sample liquid receiving part 50 are limited, the tube resistance of the sample liquid receiving part 50 is improved, the backflow of the focusing liquid into the sample liquid receiving part 50 is reduced, the condition of reagent pollution caused to the sample liquid receiving part 50 and the sample liquid conveying main tube 20 connected with the sample liquid receiving part 50 is generated, the safety of the sample detection device 1 is improved, and the detection accuracy of the sample detection device 1 is ensured.

Wherein the inner diameter of the sample liquid receiver 50 may be 0.16mm, 0.18mm, 0.20mm, 0.21mm, 0.23mm, 0.24mm, etc.; the length of the sample liquid receiver 50 may be 60mm, 65mm, 70mm, 78mm, 84mm, 90mm, 95mm, 100mm, etc.; the roughness of the inner wall of the sample liquid receiver 50 may be 0.4Ra, 0.6Ra, 0.8Ra, 0.85Ra, 1.0Ra, 1.3Ra, 1.6Ra, etc.

In this embodiment, in order to increase the resistance of the sample liquid receiving member 50, the inner diameter of the sample liquid receiving member 50 may be set to 0.16 to 0.24mm; alternatively, the length of the sample liquid receiving member 50 is set to 60 to 100mm; alternatively, the roughness of the inner wall of the sample liquid receiving member 50 is set to 0.4 to 1.6Ra. In another embodiment, in order to further increase the resistance of the sample liquid receiving member 50, the inner diameter of the sample liquid receiving member 50 may be set to 0.16 to 0.24mm, and the length of the sample liquid receiving member 50 may be set to 60 to 100mm; alternatively, the inner diameter of the sample liquid receiving member 50 is set to 0.16 to 0.24mm, and the roughness of the inner wall of the sample liquid receiving member 50 is set to 0.4 to 1.6Ra; alternatively, the length of the sample liquid receiving member 50 is set to 60 to 100mm, and the roughness of the inner wall of the sample liquid receiving member 50 is set to 0.4 to 1.6Ra; alternatively, the inner diameter of the sample liquid receiving member 50 is set to 0.16 to 0.24mm, the length of the sample liquid receiving member 50 is set to 60 to 100mm, and the roughness of the inner wall of the sample liquid receiving member 50 is set to 0.4 to 1.6Ra. The device parameters of the sample fluid receiving member 50 may be modified according to the needs of the user, and the present application is not limited thereto.

Optionally, referring to fig. 2, fig. 2 is a schematic structural diagram of a second embodiment of the sample detection device of the present application. The sample testing device 1 further includes a first three-way joint 22, and the first power device 31, the sample fluid receiving member 50, and the first sub-pipe section 21 on the sample fluid delivery main pipe 20 are respectively connected to three ends of the first three-way joint 22.

The access point of the sample liquid receiving part 50 and the sample liquid conveying main pipe 20 is located between the outflow end of the sample liquid conveying main pipe 20 and the access points of the sample liquid reaction unit 10 and the sample liquid conveying main pipe 20; the sub-pipe section of the sample fluid delivery main pipe 20 between the outflow end of the sample fluid delivery main pipe 20 and the access point of the sample fluid receiver 50 on the sample fluid delivery main pipe 20 is the second sub-pipe section 23. In this embodiment, the inner diameter of the second sub-pipe section 23 may be larger than the inner diameter of the first sub-pipe section 21. Wherein the inner diameter of the second sub-pipe section 23 may be in a multiple relationship with the inner diameter of the first sub-pipe section 21, e.g. the inner diameter of the second sub-pipe section 23 is 2 times the inner diameter of the first sub-pipe section 21, etc.

In one embodiment, the sample detection device 1 performs sample detection on the first sample liquid conveyed by the sample liquid receiving element 50 and the focusing liquid receiving element 40, and at the same time, the other sample liquid reaction unit 10 may prepare a second sample liquid, and convey the second sample liquid into the first sub-pipe section 21 of the sample liquid conveying main pipe 20 to wait. Wherein the first sample fluid and the second sample fluid are different types of sample fluids. When the sample detection device 1 finishes detecting the first sample liquid, the control of devices such as a liquid path valve, a pump and the like which may exist causes the backflow of the first sample liquid remained in the sample liquid receiving piece 50 at the moment; under the same pushing pressure, the smaller the inner diameter of the pipeline is, the larger the pipe resistance of the pipeline is, so that the pipe resistance of the first sub-pipe section 21 is larger than the pipe resistance of the second sub-pipe section 23, and when the sample liquid in the sample liquid receiving piece 50 connected between the first sub-pipe section 21 and the second sub-pipe section 23 flows backwards, the sample liquid poured out of the sample liquid receiving piece 50 can enter the second sub-pipe section 23 instead of the first sub-pipe section 21 at first due to the difference of the pipe resistances, thereby avoiding the pollution of the first sample liquid flowing backwards to the second sample liquid in the first sub-pipe section 21 and improving the safety of the sample detection device 1. Further, the second sub-pipe section 23 may be connected to a waste liquid tank for collecting the backflow sample liquid or the sample liquid which is not detected.

Alternatively, the capacity of the first sub-tube segment 21 is larger than the maximum value among the sample amounts supplied by one detection of at least two sample liquid reaction units 10.

Among them, the plurality of sample liquid reaction units 10 are used for preparing a plurality of different types of sample liquids for performing different sample detection items, for example, sample liquids for performing RET (reticulocyte) detection, sample liquids for performing PLT-F (platelet count) detection, and the like. The amount of the sample liquid needed in the detection process is different, so that the sample amount injected into the sample liquid conveying main pipe 20 is different, the capacity of the first sub-pipe section 21 is larger than the maximum value of the sample amount provided by one-time detection of all the sample liquid reaction units 10 in the sample detection device 1, thereby ensuring that the first sub-pipe section 21 can accommodate the sample amounts injected by different sample liquid reaction units 10 once, ensuring that the sample liquid reaction units 10 convey sample liquid pipelines smoothly during sample detection, ensuring that all the conveyed sample liquid enters the first sub-pipe section 21, preventing the sample liquid from blocking other pipe sections of the sample liquid conveying main pipe 20, and causing reagent pollution caused by backflow of the sample liquid into other sample liquid reaction units 10 due to pressure, and improving the safety of the sample detection device 1.

Optionally, the subsection of the sample fluid delivery main pipe 20 between the access points of the adjacent sample fluid reaction units 10 on the sample fluid delivery main pipe 20 is the third subsection 24.

The inner diameter of the third sub-pipe section 24 may be greater than or equal to the inner diameter of the first sub-pipe section 21, so that the pipe resistance of the third sub-pipe section 24 is smaller than or equal to the pipe resistance of the first sub-pipe section 21, that is, the flow rate of the sample liquid in the third sub-pipe section 24 is faster than or equal to the flow rate of the sample liquid in the first sub-pipe section 21 under the same propelling pressure, so that the time difference between the injection of the sample liquid into the first sub-pipe section 21 by each sample liquid reaction unit 10 is reduced, and the consistency between different sample liquid reaction units 10 is maintained. In addition, the tube length of the third sub-tube segment 24 may be less than 3cm, further reducing inconsistencies between different sample fluid reaction units 10.

Optionally, referring to fig. 3, fig. 3 is a schematic structural diagram of a first embodiment of the sample liquid reaction unit according to the present application. The sample liquid reaction unit 10 is connected with the sample liquid conveying main pipe 20 through a conveying branch pipe, and the conveying branch pipe comprises a first conveying branch pipe 11, a two-way valve 12 and a second conveying branch pipe 13.

Wherein the sample liquid reaction unit 10 is communicated with one end of a first conveying branch pipeline 11; the other end of the first conveying branch pipeline 11 is communicated with one end of a two-way valve 12; the other end of the two-way valve 12 is communicated with one end of a second conveying branch pipeline 13; the other end of the second transfer branch pipe 13 communicates with the sample liquid transfer main pipe 20 through the first end of the second three-way joint 14.

In the present embodiment, the two-way valve 12 may control the first transfer branch line 11 and the second transfer branch line 13 to communicate, so that the sample liquid reaction unit 10 injects the prepared sample liquid into the first sub-pipe section 21. At least two sample liquid reaction units 10 are provided with corresponding conveying branch pipes. Wherein the length of the first transfer branch line 11 is smaller than the first set length and/or the length of the second transfer branch line 13 is smaller than the second set length. The first and second set lengths may be 5cm, while the lengths of the first and second transfer branch lines 11 and 13 are preferably 3cm.

The sample liquid reaction unit 10 conveys the sample liquid into the first sub-pipe section 21 through the first conveying branch pipe 11 and the second conveying branch pipe 13, the sample liquid can be partially hung on the first conveying branch pipe 11 and the second conveying branch pipe 13 when passing through the first conveying branch pipe 11 and the second conveying branch pipe 13, if the lengths of the first conveying branch pipe 11 and the second conveying branch pipe 13 are too large, the amount of the sample liquid hanging is increased, the waste of the sample liquid is caused, the sample amount injected into the first sub-pipe section 21 by the sample liquid reaction unit 10 is influenced, and then the sample detection result of the sample detection device 1 on the sample liquid is influenced. In this embodiment, the length of the first conveying branch pipe 11 is smaller than the first set length, and/or the length of the second conveying branch pipe 13 is smaller than the second set length, so that the waste of the sample liquid is reduced, and the detection accuracy of the sample detection device 1 is improved.

Further, when the cleaning liquid is pushed to the sample liquid reaction unit 10 to clean the sample liquid reaction unit 10, if the lengths of the first conveying branch pipe 11 and the second conveying branch pipe 13 are large, that is, the resistances of the first conveying branch pipe 11 and the second conveying branch pipe 13 are large, the pushing pressure of the second power device 32 for pushing the cleaning liquid to the sample liquid reaction unit 10 will be affected, so that the cleaning efficiency of the sample liquid reaction unit 10 will be affected due to the too small pushing pressure.

In addition, since the second branch conveying pipe 13 is connected to the main conveying pipe 20 through the second three-way joint 14, that is, the sample liquid in the main conveying pipe 20 can directly reach the second branch conveying pipe 13 through the second three-way joint 14 (it can be understood that the sample liquid in the main conveying pipe 20 is not the sample liquid prepared by the sample liquid reaction unit 10 corresponding to the second branch conveying pipe 13, and is the same type of sample liquid, for convenience of understanding, the sample liquid in the main conveying pipe 20 is hereinafter referred to as the first sample liquid, the sample liquid prepared by the sample liquid reaction unit 10 corresponding to the second branch conveying pipe 13 is the second sample liquid), if the length of the second branch conveying pipe 13 is too large, the first sample liquid is likely to be accumulated in the second branch conveying pipe 13, so that when the second sample liquid is injected into the main conveying pipe 20 through the second branch conveying pipe 13, the second sample liquid is polluted, and the sample detection accuracy of the second sample liquid is reduced.

In summary, the embodiment of the present application proposes that the length of the first conveying branch pipe 11 is smaller than the first set length; and/or the length of the second conveying branch line 13 is smaller than the second set length; thereby reducing the consumption of the sample liquid by the conveying branch pipe, improving the safety and detection accuracy of the sample detection device 1, and improving the cleaning efficiency of the sample detection device 1 to the sample liquid reaction unit 10 and the conveying branch pipe.

Optionally, with continued reference to fig. 2, the sub-section of the sample fluid delivery main 20 between the point of attachment of the second power device 32 to the sample fluid delivery main 20 and the point of attachment of the sample fluid reaction unit 10 closest to the second power device 32 to the sample fluid delivery main 20 is the fourth sub-section 25.

Wherein the inner diameter of the fourth sub-pipe segment 25 is greater than or equal to the inner diameter of the third sub-pipe segment 24.

In this embodiment, since the third sub-pipe section 24 is a sub-pipe section for conveying the sample liquid, and the fourth sub-pipe section 25 is a sub-pipe section without conveying the sample liquid, the inner diameter of the fourth sub-pipe section 25 is set larger than the inner diameter of the third sub-pipe section 24, that is, the pipe resistance of the fourth sub-pipe section 25 is smaller than the pipe resistance of the third sub-pipe section 24, in the sub-pipe section without conveying the sample liquid, the loss of the pushing pressure provided by the second power device 32 is reduced, and further the pushing pressure provided by the second power device 32 for the sample liquid is ensured, and after the pressure drop along the way of each pipeline is consumed, the pushing pressure at the sample liquid receiving member 50 still can reach the requirement of the pushing pressure of the sample liquid when the sample detecting device 1 detects the sample, so that the detecting efficiency of the sample detecting device 1 is improved.

In an embodiment, if the inner diameter of the fourth sub-pipe segment 25 is equal to the inner diameter of the third sub-pipe segment 24, the length of the fourth sub-pipe segment 25 may be equal to the third preset length; in another embodiment, if the inner diameter of the fourth sub-pipe segment 25 is equal to the inner diameter of the third sub-pipe segment 24, the length of the fourth sub-pipe segment 25 may be greater than the third predetermined length, and the third predetermined length may be 50cm. When the inner diameter of the fourth sub-pipe section 25 is larger than the inner diameter of the third sub-pipe section 24, that is, the resistance of the fourth sub-pipe section 25 is smaller than the resistance of the third sub-pipe section 24, the length of the fourth sub-pipe section 25 can be properly extended, so that the layout of the second power device 32 is facilitated; when the inner diameter of the fourth sub-pipe section 25 is equal to the inner diameter of the third sub-pipe section 24, the larger the pipe length is, the larger the pipe resistance is, and the pipe length of the fourth sub-pipe section 25 should not be excessively large in order to ensure that the pipe resistance of the fourth sub-pipe section 25 is smaller than that of the third sub-pipe section 24.

Optionally, referring to fig. 4, fig. 4 is a schematic structural diagram of a third embodiment of the sample detection device of the present application. The sample detection device 1 provided by the embodiment of the present application further includes a focusing fluid supply unit 60 and a rectifying flow channel 61.

The focusing fluid supply unit 60 is connected to the focusing fluid receiving member 40 through the rectifying flow channel 61, and the extending direction of the focusing fluid outlet of the rectifying flow channel 61 is the same as the extending direction of the focusing fluid receiving member 40, and the extending direction of the focusing fluid outlet of the rectifying flow channel 61 and the extending direction of the focusing fluid receiving member 40 are both the first direction X. In this embodiment, after the focusing fluid enters the focusing fluid receiving element 40 through the rectifying flow channel 61, the focusing fluid flows in the focusing fluid receiving element 40 along the extending direction of the focusing fluid receiving element 40, that is, the first direction X, and wraps the sample fluid until the sample fluid receiving element 50 is reached, but if the extending direction of the focusing fluid outlet of the rectifying flow channel 61 is not the first direction X, but forms an included angle with the first direction X, that is, when the focusing fluid enters the focusing fluid receiving element 40, the flowing direction forms an included angle with the first direction X, at this time, the focusing fluid needs to be redirected, so that the focusing fluid flows along the first direction X, and when the focusing fluid is diverted, the fluid path of the focusing fluid is easily disturbed, thereby affecting the wrapping effect of the focusing fluid on the sample fluid, and further affecting the detection efficiency of the sample detection device 1 on the sample fluid.

Therefore, in this embodiment, the extension direction of the focusing fluid outlet of the rectifying channel 61 is the same as the extension direction of the focusing fluid receiving member 40, so that the focusing fluid is ensured to enter the focusing fluid receiving member 40, and the stability of wrapping the sample fluid is ensured, and the detection efficiency of the sample detection device 1 is improved.

The extending direction of the focusing fluid inlet of the flow straightening channel 61 may be perpendicular to the extending direction of the outlet of the flow straightening channel 61, that is, the extending direction of the focusing fluid inlet of the flow straightening channel 61 is perpendicular to the first direction X. Thereby facilitating the sample detection device 1 to set the focusing fluid supply unit 60 at the whole position, the focusing fluid supply unit 60 does not need to be set in the first direction X, simplifying the device setting structure in the sample detection device 1, and reducing the structural cost of the sample detection device 1.

Wherein the driving pressure of the focus fluid supply unit 60 is greater than the set pressure value. In this embodiment, the set pressure value may be 70KPa, and the driving pressure of the focusing fluid supply unit 60 is greater than 70KPa, for example, the driving pressure of the focusing fluid supply unit 60 is 90KPa, 160KPa, etc.; thereby, when the focusing liquid is driven to flow in the focusing liquid receiving part 40 and reaches the outlet of the sample liquid receiving part 50, the driving pressure of the focusing liquid is larger than the pushing pressure of the sample liquid, the sample liquid is extruded, so that sample liquid cells in the formed focusing sample liquid laminar flow are arranged one by one, the sample detection device 1 is convenient for carrying out sample detection on the sample liquid, and the detection accuracy of the sample detection device 1 is improved.

Optionally, referring to fig. 5, fig. 5 is a schematic structural diagram of a fourth embodiment of the sample detection device of the present application. The focusing fluid receiving member 40 is further provided with an acceleration flow path 41 and a detection flow path 42, and the focusing fluid supply unit 60 injects the focusing fluid through the rectification flow path 61, and the focusing fluid flows through the rectification flow path 61 to the acceleration flow path 41 and the detection flow path 42.

The acceleration flow path 41 accelerates the focused liquid while flowing therethrough, and the detection flow path 42 detects the sample by forming a focused sample liquid laminar flow with the focused liquid. Wherein the radial dimension D1 of the rectifying flow channel 61 is larger than the radial dimension D3 of the detecting flow channel 42, the accelerating flow channel 41 is respectively communicated with the rectifying flow channel 61 and the detecting flow channel 42, and the radial dimension of the accelerating flow channel 41 is gradually reduced from one end of the rectifying flow channel 61 to one end of the detecting flow channel 42, as shown in fig. 5, the radial dimension D2 of the accelerating flow channel 41 at one end connected with the rectifying flow channel 61 is gradually reduced to the radial dimension D3 of the other end of the accelerating flow channel 41 connected with the detecting flow channel 42, so as to be connected with the detecting flow channel 42. The focusing liquid flows out of the rectifying flow channel 61 with the radial dimension D1 and reaches the accelerating flow channel 41 with the variable radial dimension, the radial dimension of the accelerating flow channel 41 is reduced from the radial dimension D2 to the radial dimension D3, and in a pipeline with the reduced radial dimension of the accelerating flow channel 41, the focusing liquid gradually increases in flow velocity under the same driving pressure due to the gradual decrease of the radial dimension of the pipeline, so that the focusing liquid is accelerated on the accelerating flow channel 41 until the focusing liquid reaches the detecting flow channel 42.

Wherein, the pipeline connection department that corresponds with the detection runner 42 of acceleration runner 41 can have the chamfer structure, and when focusing fluid flowed to the detection runner 42 from acceleration runner 41, utilize the chamfer structure to lead the focusing fluid to detect runner 42, slowly turn to the focusing fluid, improve the stability that the focusing fluid flowed.

The outlet of the sample liquid receiving element 50 is also located at the junction of the accelerating flow channel 41 and the detecting flow channel 42 of the focusing liquid receiving element 40, where the distance between the outlet of the sample liquid receiving element 50 and the inlet of the detecting flow channel 42 is smaller than the set distance, in this embodiment, the set distance may be 0.03mm-3mm, that is, the sample liquid is output at the junction of the accelerating flow channel 41 and the detecting flow channel 42, at this time, the accelerated focusing liquid presses the sample liquid output by the sample liquid receiving element 50, so that the cells of the sample liquid pass through the detecting flow channel 42 one by one in the focused sample liquid laminar flow, where the radial dimension D1 of the rectifying flow channel 61 is larger than the radial dimension D3 of the detecting flow channel 42, so as to further ensure that the sample liquid cells in the focused sample liquid laminar flow passing through the detecting flow channel 42 are arranged one by one. And the roughness of the inner wall of the pipeline corresponding to the detection flow channel 42 may be smaller than the set roughness threshold, in this embodiment, the set roughness threshold may be smaller than 0.8Ra, and more preferably 0.4Ra, so that the pressure drop along the pipeline of the formed focused sample liquid laminar flow in the detection flow channel 42 is reduced, the focused sample liquid laminar flow can pass through the detection flow channel 42 without being blocked, and the detection efficiency of the sample detection device 1 is improved.

Further, the radial dimension of the acceleration flow channel 41 is smaller than 40 ° from the radial dimension D2 toward the extending direction of the pipeline with the radial dimension D3, and the axial extending direction of the pipeline with the detection flow channel 42 (i.e. the first direction X), so that the focusing liquid is slowly and stably accelerated in the acceleration flow channel 41; if the included angle is too large, that is, the situation that the focusing fluid may have a sudden change of flow velocity in the acceleration flow channel 41, the fluid path is easy to be disturbed, so that the focusing fluid and the sample fluid form a focused sample fluid laminar flow, and further the sample detection is affected. In this embodiment, the radial dimension of the acceleration channel 41 is set from the radial dimension D2 to the extending direction of the pipeline with the radial dimension D3, and the included angle between the radial dimension and the axial extending direction of the pipeline with the detection channel 42 is smaller than 40 °, so that the stability of the focused liquid channel is improved, and the detection efficiency of the sample detection device 1 is improved.

Alternatively, referring to fig. 6, fig. 6 is a schematic structural view of a first embodiment of the focusing fluid supply unit of the present application. The focus fluid supply unit 60 provided by the embodiment of the present application includes a first focus fluid pipe 62 and a second focus fluid pipe Jiao Ye pipe 63 connected in parallel to each other.

The pipe diameter of the first focusing fluid pipe 62 is larger than the pipe diameter of the second focusing fluid pipe Jiao Ye pipe 63, that is, the pipe pressure drop along the second focusing fluid pipe 63 is larger than the pipe pressure drop along the first focusing fluid pipe 62 under the same driving pressure, and the focusing fluid supply unit 60 can control the focusing fluid to be input into the focusing fluid receiving member 40 through the first focusing fluid pipe 62 or the second focusing fluid pipe 63, so as to supply the focusing fluid to the focusing fluid receiving member 40 under different driving pressures.

The first focusing fluid piping 62 and the second focusing fluid piping 63 can be turned on and off independently of each other. In this embodiment, the two-way valves capable of controlling the opening and closing of the pipeline may be respectively disposed on the first focusing fluid pipeline 62 and the second focusing fluid pipeline 63, the two-way valve disposed on the first focusing fluid pipeline 62 may control the opening and closing of the first focusing fluid pipeline 62, the two-way valve disposed on the second focusing fluid pipeline Jiao Ye may control the opening and closing of the second focusing fluid pipeline 63, and the two-way valves are independent of each other and do not interfere with each other, so that the first focusing fluid pipeline 62 and the second focusing fluid pipeline 63 may be opened and closed independently of each other.

Further, the focusing fluid may be supplied to the focusing fluid receiver 40 via the first focusing fluid line 62 and/or the second focusing fluid line Jiao Ye 63, which are in an on state. Since the pipe diameter of the first focusing fluid pipe 62 is larger than the pipe diameter of the second focusing fluid pipe Jiao Ye pipe 63, that is, when the focusing fluid supply unit 60 drives the focusing fluid at the same driving pressure, the driving pressure when the focusing fluid flows through the first focusing fluid pipe 62 in the conducting state and enters the focusing fluid receiving member 40 is larger than the driving pressure when the focusing fluid flows through the second focusing fluid pipe Jiao Ye 63 in the conducting state and enters the focusing fluid receiving member 40.

In practical application, when different sample liquids are detected, focusing liquids with different driving pressures are required to be wrapped, so that a better detection effect can be realized. When the focusing fluid having a small driving pressure is required, the focusing fluid supply unit 60 may supply the focusing fluid to the focusing fluid receiver 40 only through the second focusing fluid Jiao Ye pipe 63; when the focus liquid having a large driving pressure is required, the focus liquid supply unit 60 may supply the focus liquid to the focus liquid receiver 40 only through the first focus liquid pipe 62; when a focus liquid of a larger driving pressure is required, the focus liquid supply unit 60 may supply the focus liquid to the focus liquid receiver 40 through both the first focus liquid pipe 62 and the second focus liquid pipe 63. Thereby realizing that the focusing fluid supply unit 60 drives the focusing fluid to the focusing fluid receiving member 40 with a plurality of driving pressures, meeting the requirements of the sample detection device 1 for the focusing fluid when detecting different sample fluids, and improving the practicability of the sample detection device 1.

Wherein, the pipe diameter of the second focusing fluid pipeline 63 can be smaller than 1mm; and the ratio of the pipe diameter of the first focusing liquid pipe 62 to the pipe diameter of the second focusing liquid pipe Jiao Ye pipe 63 is 2 to 8. In this embodiment, the pipe diameter of the second focusing fluid pipe 63 may be smaller than 1mm, and the ratio of the pipe diameter of the first focusing fluid pipe 62 to the pipe diameter of the second focusing fluid pipe 63 is 6, that is, the pipe diameter of the first focusing fluid pipe 62 is smaller than 2mm. For example, if the pipe diameter of the second focusing fluid pipe line 63 is 0.5mm, the pipe diameter of the first focusing fluid pipe line 62 is 2mm; in other embodiments, the ratio of the pipe diameter of the first focusing fluid pipe 62 to the pipe diameter of the second focusing fluid pipe 63 may be 2, 3, 3.5, 4, 7, 7.4, 8, etc., so as to obtain different pipe diameters of the first focusing fluid pipe 62 and the second focusing fluid pipe 63, and further obtain different driving pressures of the focusing fluid supply unit 60, thereby meeting the detection requirements of different sample fluids of the sample detection device 1 and further improving the practicality of the sample detection device 1.

Alternatively, the inner diameter of the tube on the side of the first focusing fluid conduit 62 near the focusing fluid receiving member 40 is different from the inner diameter of the tube on the side of the first focusing fluid conduit 62 far from the focusing fluid receiving member 40.

Specifically, the inner diameter of the tube on the side of the first focusing fluid conduit 62 close to the focusing fluid receiving member 40 may be smaller than the inner diameter of the tube on the side of the first focusing fluid conduit 62 away from the focusing fluid receiving member 40. Thus, the focusing fluid is accelerated in advance when flowing through the first focusing fluid pipe 62, and the detection efficiency of the sample detection device 1 is improved.

In summary, the sample detection device 1 provided in the embodiment of the present application includes at least two sample fluid reaction units 10, a sample fluid delivery main pipe 20, a first power device 31, a second power device 32, a focusing fluid receiving member 40, a sample fluid receiving member 50, and a focusing fluid supply unit 60. Various parameters of the sample liquid receiving element 50 are limited, so that the tube resistance of the sample liquid receiving element 50 is improved, the condition that the focusing liquid flows back into the sample liquid receiving element 50 is avoided, and the safety of the sample detection device 1 is improved. Parameters of each sub-pipe section on the main sample liquid conveying pipe 20 are limited, so that the stability of the sample liquid in the conveying process is improved, and the detection efficiency of the sample detection device 1 is improved. In addition, parameters of the focusing fluid receiving member 40 for receiving the focusing fluid and the sample fluid are defined, so that the focusing fluid is accelerated in the focusing fluid receiving member 40 and wraps the sample fluid, a focused sample fluid laminar flow is formed to pass through the detection flow channel 42 of the focusing fluid receiving member 40, cells of the sample fluid are arranged one by one for sample detection, and detection accuracy of the sample detection device 1 is improved. Further, the focusing fluid supply unit 60 further includes a first focusing fluid pipe 62 and a second focusing fluid pipe Jiao Ye pipe 63 having different pipe diameters, so that the focusing fluid supply unit 60 can supply the focusing fluid to the focusing fluid receiving member 40 at different driving pressures, thereby improving the practicality of the sample detection apparatus 1.

The foregoing description is only of embodiments of the present application, and is not intended to limit the scope of the application, and all equivalent structures or equivalent processes using the descriptions and the drawings of the present application or directly or indirectly applied to other related technical fields are included in the scope of the present application.

Claims

1. A sample detection device is characterized in that,

and the inner diameter of the sample liquid receiving part is 0.16-0.24mm; and, the sample liquid receiving member has a length of 60-100mm; and, the roughness of the inner wall of the sample liquid receiving member is 0.4-1.6Ra;

The sample detection device comprises a rectifying flow channel, the focusing liquid receiving piece further comprises an accelerating flow channel and a detection flow channel, and the focusing liquid flows to the accelerating flow channel and the detection flow channel through the rectifying flow channel;

the radial dimension of the flow straightening channel is larger than that of the detection channel, the flow accelerating channel is respectively communicated with the flow straightening channel and the detection channel, and the radial dimension of the flow accelerating channel is gradually reduced from one end of the flow accelerating channel connected with the flow straightening channel to the other end of the flow accelerating channel connected with the detection channel.

2. The sample testing device of claim 1, further comprising a first three-way joint;

3. The sample testing device of claim 2, wherein,

the first sub-section has a capacity greater than a maximum of the sample volumes provided by the at least two sample fluid reaction units for one test.

4. The sample testing device of claim 2, wherein,

the sub-pipe section of the sample liquid conveying main pipe between the access points of the adjacent sample liquid reaction units on the sample liquid conveying main pipe is a third sub-pipe section

5. The sample testing device of claim 2, wherein,

6. The sample testing device of claim 4, wherein,

the sub-pipe section of the sample liquid conveying main pipe between the access point of the second power device on the sample liquid conveying main pipe and the access point of the sample liquid reaction unit closest to the second power device on the sample liquid conveying main pipe is a fourth sub-pipe section,

7. The sample testing device of claim 1, wherein,

the sample detection device further includes a focusing fluid supply unit,

8. The sample testing device of claim 7, wherein,

and an included angle between the extending direction of the pipeline of the accelerating flow channel and the axial extending direction of the pipeline of the detecting flow channel is smaller than 40 degrees.

9. The sample testing device of claim 7, wherein,

the focusing fluid supply unit includes a first focusing fluid line and a second focusing fluid line Jiao Ye connected in parallel to each other;

10. The sample testing device of claim 9, wherein an inner diameter of a tube on a side of said first focusing fluid conduit adjacent said focusing fluid receiving member is different from an inner diameter of a tube on a side of said first focusing fluid conduit remote from said focusing fluid receiving member.