CN116349629A - A method for indoor intensive cultivation of Macrobrachium rosenbergii species - Google Patents

Abstract

The invention provides an indoor intensive cultivation method for Macrobrachium rosenbergii species, which belongs to the technical field of aquaculture. The method comprises the following steps: disinfecting the greenhouse and the shrimp breeding pond, injecting culture water into the shrimp breeding pond, putting Macrobrachium rosenbergii seedlings in the shrimp breeding pond in the first ten days of October, and feeding Macrobrachium rosenbergii basic feed and nutrition every day Reinforcement, once in the morning and once in the evening, was continuously fed for 60 days, and the microbial preparation was sprinkled into the shrimp pond for 10 consecutive days; the stocking density of Macrobrachium rosenbergii species was 1.0‑1.5kg/m ³ . The breeding method of Macrobrachium rosenbergii species of the present invention sets reasonable stocking density, sprinkles microbial preparations, feeds Macrobrachium rosenbergii basal feed and adds nutritional fortifier beef, thereby improving the body weight and oviposition rate of the Macrobrachium rosenbergii female parents, Accelerating the development of female shrimp ovaries and shortening the development time can also improve the activity of protease in the intestinal tract of Macrobrachium rosenbergii parents and the individual antioxidant capacity.

Description

A method for indoor intensive cultivation of Macrobrachium rosenbergii species

technical field

The invention belongs to the technical field of aquaculture, and in particular relates to an indoor intensive cultivation method for Macrobrachium rosenbergii species.

Background technique

Macrobrachium rosenbergii (Macrobrachium rosenbergii), also known as Malaysian prawn, is the main freshwater shrimp cultured in the world. It has been successfully cultured in Guangdong, Guangxi, Hunan, Hubei, Zhejiang and other places, with remarkable economic benefits. In recent years, affected by unfavorable factors such as germplasm degradation and poor stress resistance, Macrobrachium rosenbergii has problems such as decreased reproductive performance of female parents, retarded ovarian development, low oviposition rate, and poor quality of larvae during the breeding process.

In order to improve the quality of Macrobrachium rosenbergii seedlings, the existing technology generally starts from the perspective of nutritional strengthening of seedlings, and uses different types, different combinations, and different dosages of nutritional enhancers to improve the ovarian development, synchronization, and oviposition rate of female parents. For example, the patent with the application number CN201910091831.7 discloses a method for increasing the oviposition rate of Macrobrachium rosenbergii, which aims to solve the ovary growth retardation of female parents in the cultivation process of Macrobrachium rosenbergii in the prior art through the nutrition enhancement of the species of Macrobrachium rosenbergii. Problems such as low oviposition rate of shrimp and poor quality of larvae. However, the reproductive performance of breeding shrimp is related to many factors. Solving the problems of delayed ovarian development and low oviposition rate of the female parents of M. Slow growth, early sexual maturity, and small individuals.

Contents of the invention

In order to solve the above-mentioned technical problems, the present invention proposes a method for intensive cultivation of Macrobrachium rosenbergii species indoors, by setting a suitable stocking density for Macrobrachium rosenbergii species in the greenhouse cultivation period, on this basis, adding nutritional fortifiers to carry out parental nutrition Strengthen and improve the degree of gonad development, and provide guidance for the cultivation of Macrobrachium rosenbergii species, seed production and improvement of breeding technology.

In order to achieve the above object, the invention provides a method for indoor intensive cultivation of Macrobrachium rosenbergii species, comprising the following steps:

Disinfect the greenhouse and shrimp ponds, inject breeding water into the shrimp ponds, put Macrobrachium rosenbergii seedlings in the shrimp ponds in early October, and feed Macrobrachium rosenbergii basic feed and nutrient-enhanced beef every day, 1 day each morning and evening. Every day, feed 1/2 of the feed in the morning, and then feed the nutritional fortifier in the afternoon, and then feed the remaining 1/2 of the feed. Continuous feeding for 60 days, the stocking density of Macrobrachium rosenbergii species is 1.0-1.5kg/m ³ .

Further, the indoor intensive cultivation method of Macrobrachium rosenbergii species can also include the step of spraying the microbial preparation into the species shrimp pond after putting Macrobrachium rosenbergii species in the species pond for 10 consecutive days.

Further, the greenhouse is fumigated and sterilized with potassium permanganate+formaldehyde, and the dosage is 10g potassium permanganate+20g formaldehyde/m ² ; the shrimp ponds are soaked with 35mg/L strong chloroquine, and soaked for disinfection After 3 days, rinse off with clean water.

Further, the total daily feeding amount of the Macrobrachium rosenbergii basal feed and the nutritional supplement is 0.5%-1% of the total mass of the Macrobrachium rosenbergii species, and the mass ratio of the Macrobrachium rosenbergii basal feed and the nutritional supplement is 4: 1. The quality of the nutritional fortifier is the mass of the dry matter in the nutritional fortifier, that is, the actual weight of the nutritional fortifier = the mass of the dry matter in the required nutritional fortifier ÷ (100% - water content).

Further, the preparation method of the nutrient-enhanced beef includes the following steps: cutting the fresh beef into pieces, steaming it with water, putting it at room temperature, and pulverizing it, and making it for immediate use.

Further, the Macrobrachium rosenbergii basic feed includes fish meal, Antarctic krill meal, fermented soybean meal, soybean protein concentrate, fish oil and phospholipid, and its basic composition (mass percentage) is crude protein ≥ 40%, crude fat ≥ 6%, crude fiber ≤5%, crude ash ≤15%, total phosphorus ≥1.0%, moisture ≤12.0%, lysine ≥2.6%.

Further, the dissolved oxygen in the breeding water is >7.0mg/L, the pH value is 7.2-7.5, and the water temperature is 24-26°C. Air stones can be hung at a distance of 70cm between the two long sides of the shrimp breeding pond, so that the dissolved oxygen in the breeding water can meet the >7.0mg/L requirement.

Further, before the culture water is injected into the shrimp pond, it is disinfected and aerated with 2 mg/L strong chlorine essence for 1 day, and it can be used only if the residual chlorine is less than 0.02 mg/L as detected by the residual chlorine detection reagent.

Further, the male-to-female ratio of the fresh Macrobrachium rosenbergii species was 2:1.

Further, the microbial preparation includes Bacillus subtilis and Bacillus licheniformis.

Further, the dosage of the microbial preparation is 0.3-0.5g/m ³ water body.

Compared with the prior art, the present invention has the following advantages and technical effects:

1. Adopt the method for intensive cultivation of Macrobrachium rosenbergii species indoors of the present invention, by setting a reasonable stocking density, and splashing microorganisms, feeding the Macrobrachium rosenbergii basic feed and adding beef with nutritional fortifiers, thereby improving the individuality of the Macrobrachium rosenbergii female parent. The quality and oviposition rate can accelerate the development of female shrimp ovary, shorten the development time and improve the activity of protease in the intestinal tract of Macrobrachium rosenbergii parents and the antioxidant capacity of individuals.

2. Adopting the stocking density set by the present invention to cultivate seedling shrimp can increase the body weight of the female parents of Macrobrachium rosenbergii by 14.15%, increase the body length by 3.73%, and increase the oviposition rate by 17.33%. Simultaneously, reasonable stocking density helps to keep good water quality, when ammonia nitrogen and nitrite nitrogen reach peak value, adopt the stocking density that the present invention sets, and combine the microbial preparation of splashing, can make ammonia nitrogen content reduce 58.7%, sub- The nitrate nitrogen content is reduced by 39.96%, which can effectively save water resources and costs before the water quality is stable. In addition, under the stocking density of 1.0-1.5kg/ ^m3 in the present invention, beef is used as a nutritional fortifier to strengthen the nutrition of the seed shrimp, and the ratio of male to female is set to 2:1, and the basic feed of Macrobrachium rosenbergii is fed every day and additional beef is added. , adopting this strengthening strategy can increase the body mass of giant Macrobrachium rosenbergii females by 28.4%. The results of ovary tissue sections show that the ovaries of the beef group are in stage IV ovaries (later stage of development), and the ovaries are larger in size, golden in color, and mature. It can be seen that the addition of beef can accelerate the development of female shrimp ovary and shorten the development time, and can also improve the activity of protease in the intestinal tract of Macrobrachium rosenbergii parents and the individual antioxidant capacity.

Description of drawings

The drawings constituting a part of the application are used to provide further understanding of the application, and the schematic embodiments and descriptions of the application are used to explain the application, and do not constitute an improper limitation to the application. In the attached picture:

Fig. 1 is the final body weight measurement result of the Macrobrachium rosenbergii female shrimp cultured in embodiment 1-2 and comparative example 1;

Fig. 2 is the body length measurement result of the Macrobrachium rosenbergii female shrimp cultured in embodiment 1-2 and comparative example 1;

Fig. 3 is embodiment 1, 3, 4 and 5 in the indoor intensive cultivation of Macrobrachium rosenbergii species Ammonia nitrogen concentration determination result in water body;

Fig. 4 is embodiment 1, 3, 4 and 5 in the nitrite nitrogen concentration measurement result in the water body in the Macrobrachium rosenbergii indoor intensive cultivation process;

Fig. 5 is embodiment 1-2 and comparative example 1 Macrobrachium rosenbergii female prawn oviposition measurement result;

Fig. 6 is embodiment 1 and comparative example 2-4 Macrobrachium rosenbergii parent body weight measurement result;

Fig. 7 is the measurement result of the macrobrachium rosenbergii ovary histological characteristics (×200) of embodiment 1 culture;

Fig. 8 is the measurement result of the macrobrachium rosenbergii ovary histological characteristics (×200) of comparative example 2 culture;

Fig. 9 is the measurement result of the macrobrachium rosenbergii ovary histological characteristics (×200) of comparative example 3 cultivation;

Fig. 10 is the measurement result of the macrobrachium rosenbergii ovary histological characteristics (×200) of comparative example 4 culture;

Fig. 11 is the activity test result of protease in the gut of the female parent of Macrobrachium rosenbergii cultured in Example 1 and Comparative Example 2-4;

Fig. 12 is the measurement result of total superoxide dismutase activity in the serum of Macrobrachium rosenbergii cultured in Example 1 and Comparative Examples 2-4;

Fig. 13 is the measurement result of serum catalase activity of Macrobrachium rosenbergii cultured in embodiment 1 and comparative examples 2-4;

Fig. 14 is the measurement results of the total antioxidant capacity of the serum of Macrobrachium rosenbergii cultured in Example 1 and Comparative Examples 2-4.

Detailed ways

Various exemplary embodiments of the present invention will now be described in detail. The detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features and embodiments of the present invention.

It should be understood that the terminology described in the present invention is only used to describe specific embodiments, and is not used to limit the present invention. In addition, regarding the numerical ranges in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded from the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only the preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and/or materials in connection with which the documents are described. In case of conflict with any incorporated document, the contents of this specification control.

It will be apparent to those skilled in the art that various modifications and changes can be made in the specific embodiments of the present invention described herein without departing from the scope or spirit of the present invention. Other embodiments will be apparent to the skilled person from the description of the present invention. The description and examples of the invention are illustrative only.

As used herein, "comprising", "comprising", "having", "comprising" and so on are all open terms, meaning including but not limited to.

The "room temperature" mentioned in the present invention refers to 25±2°C unless otherwise specified.

The invention provides a method for indoor intensive cultivation of Macrobrachium rosenbergii species, comprising the following steps:

Disinfect the greenhouse and shrimp ponds, inject culture water into the shrimp ponds, put Macrobrachium rosenbergii species in the shrimp ponds in early October (the temperature difference between the outer pond and the greenhouse water should not exceed 3°C), and feed Macrobrachium rosenbergii every day Shrimp basic feed and nutrient fortifier, once in the morning and evening, feed 1/2 of the feed amount every morning, feed the nutrient fortifier first in the afternoon, and then feed the remaining 1/2 feed, and feed continuously for 60 days; Macrobrachium rosenbergii species The stocking density of shrimp is 1.0-1.5kg/m ³ .

In the embodiment of the present invention, the method for intensive cultivation of Macrobrachium rosenbergii indoors may also include the step of spraying the microbial preparation into the pond for 10 consecutive days after putting the Macrobrachium rosenbergii seed in the pond.

In principle, the present invention has no special restrictions on the disinfection method of greenhouses and shrimp ponds. In the embodiment of the present invention, the greenhouse is fumigated and sterilized with potassium permanganate+formaldehyde, and the dosage is 10g potassium permanganate+20g formaldehyde/m ² ; The described shrimp pond is soaked in full water with 35mg/L strong chlorine essence, after soaking and disinfecting for 3 days, rinse with clear water.

In the embodiment of the present invention, the greenhouse shrimp pond is a cement pond with a length of 5.5m, a width of 3.0m, and a height of 1.0m. The actual water depth in each shrimp pond during the breeding process is 0.7m, and the effective water body is 11.55m ³ .

In an embodiment of the present invention, the total daily feeding amount of the Macrobrachium rosenbergii basal feed and nutritional supplements is 0.5%-1% of the total mass of Macrobrachium rosenbergii species, and the mass of Macrobrachium rosenbergii basal feed and nutritional supplements The ratio is 4:1, the quality of the nutritional fortifier is the mass of the dry matter in the nutritional fortifier, that is, the actual weight of the nutritional fortifier = the mass of the dry matter in the required nutritional fortifier ÷ (100% - moisture content) .

In the embodiment of the present invention, the nutritional fortifier is beef, and the preparation method specifically includes the following steps: cut 500g of fresh beef into pieces, add 1L of water, cook in boiling water for 20min, put it at room temperature, and chop it with a knife until the particle size is Small pieces of 0.2-0.3cm are used and made now, and the water content is 76.2% after testing, that is, the actual weight of the nutritional fortifier = the mass of dry matter in the required nutritional fortifier ÷ (100% - 76.2%) .

In an embodiment of the present invention, the Macrobrachium rosenbergii basic feed includes fish meal, Antarctic krill meal, fermented soybean meal, soybean protein concentrate, fish oil and phospholipid, and the basic composition (mass percentage) is crude protein ≥ 40%, crude fat ≥ 6% %, crude fiber ≤ 5%, crude ash ≤ 15%, total phosphorus ≥ 1.0%, moisture ≤ 12.0%, lysine ≥ 2.6%.

In the embodiment of the present invention, the dissolved oxygen in the culture water is >7.0mg/L, the pH value is 7.2-7.5, and the water temperature is 24-26°C. Air stones can be hung at a distance of 70cm from the two long sides of the breeding pond to make the culture Dissolved oxygen in water meets the requirement of >7.0mg/L, and a 1.2-meter-wide mesh is hung at a depth of 30cm and 50cm in the shrimp pond.

In the embodiment of the present invention, before the culture water is injected into the shrimp pond, it is disinfected and aerated with 2 mg/L strong chlorine essence for 1 day, and it can be used only when the residual chlorine is less than 0.02 mg/L as detected by the residual chlorine detection reagent.

In the embodiment of the present invention, the ratio of male to female in the species of Macrobrachium rosenbergii was 2:1.

In the embodiment of the present invention, the microbial preparations include Bacillus subtilis and Bacillus licheniformis, specifically the microbial preparation Yumebo purchased from Yubang Biotechnology (Shanghai) Co., Ltd., wherein Bacillus subtilis, Bacillus licheniformis and Saccharomyces cerevisiae The mass ratio is 1:1:1.

In the embodiment of the present invention, the dosage of the microbial preparation is 0.3-0.5 g/m ³ water body.

In the embodiment of the present invention, the individual average body mass of Macrobrachium rosenbergii species is 19.7 g/head.

The technical solution of the present invention will be further described below through examples.

Example 1

The greenhouse is fumigated and disinfected with potassium permanganate + formaldehyde, the dosage is 10g potassium permanganate + 20g formaldehyde/ ^m2 ; the shrimp pond is a cement pond, the size is 5.5m long, 3.0m wide, and 1.0m high, with 35mg/m Soak the whole pool of L strong chlorine essence in water, rinse it with clean water after soaking and disinfecting for 3 days;

Disinfect and aerate the breeding water with 2mg/L strong chlorine essence for 1 day, and the residual chlorine is 0.01mg/L as detected by the residual chlorine detection reagent. Hang a 1.2-meter-wide mesh at a depth of 30cm and 50cm in the shrimp breeding pond, and there are two ponds Air stones were hung at intervals of 70cm on the long side, and water for cultivation was injected into the shrimp pond (adjusting dissolved oxygen to 7.3mg/L, pH value to 7.4, water temperature to 26°C), water depth to 0.7m, and effective water body to 11.55m ³ . Every day (the temperature difference between the outer pond and the greenhouse does not exceed 3°C), the species of Macrobrachium rosenbergii with a ratio of male to female of 2:1 is placed in the breeding pond, and the stocking density is 1.0kg/m ³ , and then the mass ratio of daily feeding is 4: 1 Macrobrachium rosenbergii basal feed (basic composition (mass percentage): crude protein 40%, crude fat 6%, crude fiber 4%, crude ash 13%, total phosphorus 1.5%, moisture 11.6%, lysine 2.9%) and nutrient fortifier (beef), the total daily feeding amount is 1% of the total mass of Macrobrachium rosenbergii species, once in the morning and evening, 1/2 of the feed amount is fed every morning, and the nutrient fortifier is fed first in the afternoon, and then Feed the remaining 1/2 of the feed for 60 consecutive days. On the first day of the shrimp cultivation, at the same time, sprinkle the microbial preparation Yumebo (a mixture of Bacillus subtilis, Bacillus licheniformis and Saccharomyces cerevisiae) in the breeding pond, the dosage is 0.3g /m ³ water body, continuously splashed for 10 days.

Example 2

Same as Example 1, the only difference is that the stocking density is 1.5kg/m ³ .

Example 3

The difference from Example 1 is only to omit the step of splashing microbial preparations in the shrimp pond, specifically:

The greenhouse is fumigated and disinfected with potassium permanganate + formaldehyde, the dosage is 10g potassium permanganate + 20g formaldehyde/ ^m2 ; the shrimp pond is a cement pond, the size is 5.5m long, 3.0m wide, and 1.0m high, with 35mg/m Soak the whole pool of L strong chlorine essence in water, rinse it with clean water after soaking and disinfecting for 3 days;

Disinfect and aerate the breeding water with 2mg/L strong chlorine essence for 1 day, and the residual chlorine is 0.01mg/L as detected by the residual chlorine detection reagent. Hang a 1.2-meter-wide mesh at a depth of 30cm and 50cm in the shrimp breeding pond, and there are two ponds Hang air stones at intervals of 70cm on the long sides, inject culture water into the shrimp pond (adjust dissolved oxygen to 7.3mg/L, pH value to 7.4, water temperature to 26°C), water depth to 0.7m, effective water body to 11.55m ³ , in early October (The water temperature difference between the outer pond and the greenhouse does not exceed 3°C.) Put giant giant prawns with a male-to-female ratio of 2:1 in the breeding pond at a density of 1.0kg/m ³ , and then feed them with a mass ratio of 4:1 every day. Macrobrachium rosenbergii basal feed (basic composition (mass percentage): crude protein 48%, crude fat 6%, crude fiber 4%, crude ash 13%, total phosphorus 1.5%, moisture 11.6%, lysine 2.9%) and Nutritional fortifier (beef), the total daily feeding amount is 1% of the total mass of Macrobrachium rosenbergii species, once in the morning and evening, 1/2 of the feed amount is fed every morning, and the nutritional fortifier is fed first in the afternoon, and then fed The remaining 1/2 feed was fed continuously for 60 days.

Example 4

Same as Example 3, the only difference is that the stocking density is 1.5kg/m ³ .

Example 5

Same as Example 3, the only difference is that the stocking density is 2kg/m ³ .

Example 6

The greenhouse is fumigated and disinfected with potassium permanganate + formaldehyde, the dosage is 10g potassium permanganate + 20g formaldehyde/ ^m2 ; the shrimp pond is a cement pond, the size is 5.5m long, 3.0m wide, and 1.0m high, with 35mg/m Soak the whole pool of L strong chlorine essence in water, rinse it with clean water after soaking and disinfecting for 3 days;

Disinfect and aerate the breeding water with 2mg/L strong chlorine essence for 1 day, and the residual chlorine is 0.01mg/L as detected by the residual chlorine detection reagent. Hang a 1.2-meter-wide mesh at a depth of 30cm and 50cm in the shrimp breeding pond, and there are two ponds Hang air stones at intervals of 70cm on the long side, inject culture water into the shrimp pond (adjust dissolved oxygen 7.3mg/L, pH value 7.2, water temperature 24°C), water depth 0.7m, effective water body 11.55m ³ , in early October ( The water temperature difference between the outer pond and the greenhouse does not exceed 3°C) in the breeding pond, put the giant giant prawns with a male to female ratio of 2:1 at a density of 1.0kg/m ³ , and then feed them with a mass ratio of 4:1 every day. Macrobrachium rosenbergii basic feed (basic composition (mass percentage): crude protein 48%, crude fat 6%, crude fiber 4%, crude ash 13%, total phosphorus 1.5%, moisture 11.6%, lysine 2.9%) and nutrition Fortifier (beef), the total daily feeding amount is 0.5% of the total mass of Macrobrachium rosenbergii species, once in the morning and evening, 1/2 of the feed amount is fed every morning, and the nutritional fortifier is fed first in the afternoon, and then the rest 1/2 of the feed was fed continuously for 60 days, and the microbial preparation Yumebo (mixture of Bacillus subtilis, Bacillus licheniformis and Saccharomyces cerevisiae) was sprinkled in the shrimp pond at a dosage of 0.5g/ ^m3 water body for 10 days.

Comparative example 1

Same as Example 1, the only difference is that the stocking density is 2.0kg/m ³ .

Comparative example 2

The same as Example 1, the only difference is that the nutrient fortifier is snail meat, and the specific preparation method is: select shelled snail meat, thaw it, rinse it with tap water, weigh 500g of the thawed snail meat, put it in boiling water and cook for 20min, Take out and drain, cut into particles with a particle size of 0.3-0.7cm, freshly made every day, and the measured water content is 62.2%. The weighed amount is calculated as dry matter, and the actual weight of the nutritional fortifier = the mass of dry matter in the required nutritional fortifier ÷ (100%-62.2%).

Comparative example 3

The same as Example 1, the only difference is that the nutritional fortifier is eggs, and the specific preparation method is: take 3 eggs, take the egg liquid after peeling, stir evenly, and add tap water according to the mass ratio of egg liquid and water as 2:1 , stir evenly, skim off the foam, put it in a steamer, and steam for 5 minutes, take the steamed egg custard, cut it into cubes with a diameter of 0.5cm for later use, make it fresh every day, and the measured water content is 75.6%. The amount of eggs weighed is converted into dry matter, and the actual weight of the nutritional fortifier = the mass of dry matter in the required nutritional fortifier ÷ (100%-75.6%).

Comparative example 4

The same as in Example 1, the only difference is that no nutrient fortifier beef is added, and the daily feeding amount of the Macrobrachium rosenbergii basal feed is 1% of the total mass of Macrobrachium rosenbergii species.

Performance Testing

1. Final body weight and body length

After breeding for 60 days, randomly select 15 giant giant rosenbergii females in the breeding pond for testing:

See Fig. 1 for the final body weight measurement results of the Macrobrachium rosenbergii female shrimp cultured by the methods of embodiment 1-2 and comparative example 1, and see Fig. 2 for the body length measurement results. As can be seen from Fig. 1 and Fig. 2, when the stocking density is 1.0 At -1.5kg/m ³ , the body weight and body length of female minced shrimp were significantly higher than those in the high-density group (2.0kg/m ³ ), indicating that the stocking density of the present invention is reasonable and can increase the body weight of the female parents of Macrobrachium rosenbergii.

2. Effects on the water quality of giant giant prawn ponds

Examples 1, 3, 4 and 5 are shown in Fig. 3 and Fig. 4 for the change results of ammonia nitrogen concentration and nitrite nitrogen concentration in the water body during the cultivation process of Macrobrachium rosenbergii species. In the initial stage of shrimp breeding, the concentration of ammonia nitrogen in the initial water body is 0.2mg/L, and the concentration of nitrite nitrogen is 0.01mg/L.

It can be seen from Figure 3 that after the shrimps were put into the ponds, the concentration of ammonia nitrogen in the water body increased rapidly, reached the peak on the 11th day, and then gradually decreased. After 17 days, the concentration of ammonia nitrogen in the water body tended to be stable at 0.3mg/ L or so. From the analysis of different breeding density groups, the concentration of ammonia nitrogen increases with the increase of breeding density, Example 5 (2.0kg/m ³ )>Example 4 (1.5kg/m ³ )>Example 3 (1.0kg/m ³ ).

It can be seen from Figure 4 that the change of the concentration of nitrite nitrogen in the water body is the same as that of ammonia nitrogen, and it rises to the peak on the 17th day, and the concentration of nitrite nitrogen in Example 5 (2.0kg/m ³ ) is as high as 5.68mg /L, greater than embodiment 4 and embodiment 3. The concentration of nitrite nitrogen in the water body of each density group dropped to the lowest on the 27th day and stabilized at about 0.3mg/L.

Example 1 (spray fungus) is based on Example 3, where the microbial preparation Yumebo (a mixture of Bacillus subtilis, Bacillus licheniformis and Saccharomyces cerevisiae) was sprayed, and it was found that the content of ammonia nitrogen and nitrite nitrogen in water can be significantly reduced.

The results in Figure 3 and Figure 4 show that when the stocking density is greater than 1.5kg/m ³ , the content of ammonia nitrogen and nitrite nitrogen in the water body is high, which is not conducive to the growth of shrimp species. In order to maintain a good water environment, a larger replacement is required. water volume. After accounting, compared with Examples 3-5, Example 3 and Example 4 had less water exchange of 0.83m ³ per pond per day than Example 5 in the breeding process, calculated according to the need for 27 days for water quality stability, and the water fee was based on the average price of 3 yuan Calculated per ton, each shrimp pond can save 22.41m ³ of water resources and save about 67 yuan in cost.

3. Determination of Oviposition Rate of Macrobrachium rosenbergii Female Shrimp

After breeding for 60 days, randomly select 15 giant giant rosenbergii females in the breeding pond for testing:

Figure 5 shows the results of determination of the oviposition rate of the female Macrobrachium rosenbergii in Example 1-2 and Comparative Example 1. It can be seen from Figure 5 that when the stocking density is 1.0kg/m ³ and 1.5kg/m ³ , the oviposition rates are 49.03% and 54.23%, respectively, significantly higher than 2.0kg/m ³ (comparative example 1). The results showed that when the stocking density was greater than 1.5kg/m ³ , continuing to increase the stocking amount could not increase the oviposition rate of female shrimp, and the oviposition rate was the highest when the stocking density was 1.0-1.5kg/m ³ .

4. Nutritional fortification effect test

After cultivating for 60 days, randomly select 15 Macrobrachium rosenbergii in the breeding pond for testing:

Embodiment 1 uses beef as the nutritional fortifier, comparative examples 2 and 3 respectively use snail meat and eggs as the nutritional fortifier, and comparative example 4 does not add the nutritional fortifier, and only feeds the basal feed as a control group (CK group). The weight of the parents of Macrobrachium rosenbergii in each group was measured, the results are shown in Figure 6, and the specific data are shown in Table 1. It can be seen from Figure 6 that adding different nutrients can increase the body weight of female parents, but has no significant effect on male parents. Among them, the final body weight of the female Macrobrachium rosenbergii in the group added with beef (Example 1) was 45.03 g, which was significantly higher than that in the CK group (Comparative Example 4).

Table 1 Body weight measurement results of Macrobrachium rosenbergii parents in each group/g

♂ ♀ Beef group (embodiment 1) 53.65 45.03 Snail meat (comparative example 2) 54.30 36.06 Egg group (comparative example 3) 53.29 42.15 CK (comparative example 4) 52.08 35.07

5. Ovarian development test

The macrobrachium rosenbergii ovary histological characteristics (×200) measurement results using the method of embodiment 1 and comparative examples 2-4 are shown in Fig. 7-10, wherein Fig. 7 is embodiment 1, Fig. 8 is comparative example 2, Fig. 9 is Comparative example 3, Fig. 10 is comparative example 4. It is known that the ovary development of Macrobrachium rosenbergii can be divided into 5 developmental stages, namely: oogonia, previtellosynthetic oocytes, endogenous vitellogenic oocytes, exogenous vitellogenic oocytes and mature oocytes. It can be seen from Figure 7-10 that the ovary development stage of Macrobrachium rosenbergii in Comparative Example 4 (CK group), Comparative Example 3 (egg group), and Comparative Example 2 (snail meat group) is stage III ovary (middle stage of development), and the center of the ovary is still Mainly oogonia, follicular cells have basically surrounded a single oocyte to form a follicular cavity. However, the ovary of Example 1 (beef group) is in stage IV ovary (later stage of development). From this stage, the number of follicular cells surrounding a single follicular cavity increases rapidly. The ovary is larger in volume and golden in color when observed with the naked eye. Yellow, high maturity. This shows that the present invention can improve the ovary development of Macrobrachium rosenbergii by adding beef as a nutritional fortifier.

6. Effects on intestinal digestive enzymes and antioxidant enzymes of Macrobrachium rosenbergii

The test results of protease activity in the intestines of the female parents of Macrobrachium rosenbergii cultured using the methods of Example 1 and Comparative Examples 2-4 are shown in FIG. 11 . As can be seen from Fig. 11, after embodiment 1 uses beef as the nutrient fortifier, the activity of protease in the intestinal tract of Macrobrachium rosenbergii female parents is significantly higher than that of other groups, which is 1.87 times higher than that of the matched group (comparative example 4), illustrating the present invention Using beef as a nutrient fortifier can improve the ability of Macrobrachium rosenbergii to digest and utilize protein.

The results of the determination of the activity of antioxidant enzymes in the serum of Macrobrachium rosenbergii cultured using the methods of Example 1 and Comparative Examples 2-4 are shown in Figures 12-14, wherein Figure 12 is the result of the determination of serum total superoxide dismutase activity, and Figure 13 is the serum Catalase activity measurement results, Figure 14 is the serum total antioxidant enzyme activity measurement results. As can be seen from Fig. 12-Fig. 14, embodiment 1 uses beef as nutrient fortifier Macrobrachium rosenbergii serum T-SOD (total superoxide dismutase), CAT (catalase), T-AOC (total antibody Oxidation capacity) and other antioxidant-related enzyme activities were significantly higher than those of the control group (comparative example 4). The above results show that the present invention uses beef as a nutritional fortifier, which can significantly improve the antioxidant capacity of Macrobrachium rosenbergii parents.

The above are only preferred specific implementation methods of the present application, but the scope of protection of the present application is not limited thereto. Anyone skilled in the art can easily think of changes or substitutions within the technical scope disclosed in the present application. All should be covered within the scope of protection of this application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims (9)

1. a kind of macrobrachium rosenbergii kind of shrimp indoor strengthening cultivation method is characterized in that, comprises the following steps: Disinfect the greenhouse and shrimp ponds, inject breeding water into the shrimp ponds, put Macrobrachium rosenbergii seedlings in the shrimp ponds in early October, and feed Macrobrachium rosenbergii basic feed and nutrient-enhanced beef every day, 1 day each morning and evening. times, feeding continuously for 60 days; the stocking density of Macrobrachium rosenbergii species is 1.0-1.5kg/m ³ . 2. a kind of Macrobrachium rosenbergii kind of shrimp indoor intensive cultivation method according to claim 1, is characterized in that, also comprises the step of splashing microbial preparation in the kind of shrimp pond after putting in Macrobrachium rosenbergii kind of shrimp in the kind of shrimp pond , the microbial preparation was continuously splashed for 10 days. 3. a kind of Macrobrachium rosenbergii indoor intensive cultivation method according to claim 1, is characterized in that, the daily total feeding amount of described Macrobrachium rosenbergii basal feed and nutrient fortifier is the total mass of Macrobrachium rosenbergii species 0.5%-1% of macrobrachium rosenbergii basal feed and nutritional fortifier in a mass ratio of 4:1. 4. a kind of Macrobrachium rosenbergii kind of shrimp indoor intensive cultivation method according to claim 3, is characterized in that, described Macrobrachium rosenbergii basic feed comprises fish meal, Antarctic krill meal, soybean meal, soybean protein concentrate, fish oil and phospholipid, The basic composition is crude protein ≥ 40%, crude fat ≥ 6%, crude fiber ≤ 5%, crude ash ≤ 15%, total phosphorus ≥ 1.0%, moisture ≤ 12.0%, lysine ≥ 2.6%. 5 . The method for intensive cultivation of Macrobrachium rosenbergii species indoors according to claim 1 , characterized in that the dissolved oxygen in the culture water is >7.0 mg/L, the pH value is 7.2-7.5, and the water temperature is 24-26° C. 6 . 6. a kind of Macrobrachium rosenbergii seedling indoor intensive cultivation method according to claim 1, it is characterized in that, before described cultivation water injects seedling pond, with 1mg/L strong chlorine essence disinfection aeration 1 day, residual chlorine <0.02mg/L. 7 . The indoor intensive cultivation method of Macrobrachium rosenbergii species according to claim 1 , wherein the ratio of male to female in the Macrobrachium rosenbergii species is 2:1. 8 . The method for intensive cultivation of Macrobrachium rosenbergii species indoors according to claim 2 , wherein the microbial preparation comprises Bacillus subtilis and Bacillus licheniformis. 9 . The method for intensive cultivation of Macrobrachium rosenbergii species indoors according to claim 8 , wherein the amount of the microbial preparation is 0.3-0.5 g/m ³ water body.

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